---
_id: '14848'
abstract:
- lang: eng
  text: Regulating protein states is considered the core function of chaperones. However,
    despite their importance to all major cellular processes, the conformational changes
    that chaperones impart on polypeptide chains are difficult to study directly due
    to their heterogeneous, dynamic, and multi-step nature. Here, we review recent
    advances towards this aim using single-molecule manipulation methods, which are
    rapidly revealing new mechanisms of conformational control and helping to define
    a different perspective on the chaperone function.
alternative_title:
- New Developments in NMR
article_processing_charge: No
author:
- first_name: F.
  full_name: Wruck, F.
  last_name: Wruck
- first_name: Mario
  full_name: Avellaneda Sarrió, Mario
  id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E
  last_name: Avellaneda Sarrió
  orcid: 0000-0001-6406-524X
- first_name: M. M.
  full_name: Naqvi, M. M.
  last_name: Naqvi
- first_name: E. J.
  full_name: Koers, E. J.
  last_name: Koers
- first_name: K.
  full_name: Till, K.
  last_name: Till
- first_name: L.
  full_name: Gross, L.
  last_name: Gross
- first_name: F.
  full_name: Moayed, F.
  last_name: Moayed
- first_name: A.
  full_name: Roland, A.
  last_name: Roland
- first_name: L. W. H. J.
  full_name: Heling, L. W. H. J.
  last_name: Heling
- first_name: A.
  full_name: Mashaghi, A.
  last_name: Mashaghi
- first_name: S. J.
  full_name: Tans, S. J.
  last_name: Tans
citation:
  ama: 'Wruck F, Avellaneda Sarrió M, Naqvi MM, et al. Probing Single Chaperone Substrates.
    In: Hiller S, Liu M, He L, eds. <i>Biophysics of Molecular Chaperones</i>. Vol
    29. Royal Society of Chemistry; 2023:278-318. doi:<a href="https://doi.org/10.1039/bk9781839165986-00278">10.1039/bk9781839165986-00278</a>'
  apa: Wruck, F., Avellaneda Sarrió, M., Naqvi, M. M., Koers, E. J., Till, K., Gross,
    L., … Tans, S. J. (2023). Probing Single Chaperone Substrates. In S. Hiller, M.
    Liu, &#38; L. He (Eds.), <i>Biophysics of Molecular Chaperones</i> (Vol. 29, pp.
    278–318). Royal Society of Chemistry. <a href="https://doi.org/10.1039/bk9781839165986-00278">https://doi.org/10.1039/bk9781839165986-00278</a>
  chicago: Wruck, F., Mario Avellaneda Sarrió, M. M. Naqvi, E. J. Koers, K. Till,
    L. Gross, F. Moayed, et al. “Probing Single Chaperone Substrates.” In <i>Biophysics
    of Molecular Chaperones</i>, edited by Sebastian Hiller, Maili Liu, and Lichun
    He, 29:278–318. Royal Society of Chemistry, 2023. <a href="https://doi.org/10.1039/bk9781839165986-00278">https://doi.org/10.1039/bk9781839165986-00278</a>.
  ieee: F. Wruck <i>et al.</i>, “Probing Single Chaperone Substrates,” in <i>Biophysics
    of Molecular Chaperones</i>, vol. 29, S. Hiller, M. Liu, and L. He, Eds. Royal
    Society of Chemistry, 2023, pp. 278–318.
  ista: 'Wruck F, Avellaneda Sarrió M, Naqvi MM, Koers EJ, Till K, Gross L, Moayed
    F, Roland A, Heling LWHJ, Mashaghi A, Tans SJ. 2023.Probing Single Chaperone Substrates.
    In: Biophysics of Molecular Chaperones. New Developments in NMR, vol. 29, 278–318.'
  mla: Wruck, F., et al. “Probing Single Chaperone Substrates.” <i>Biophysics of Molecular
    Chaperones</i>, edited by Sebastian Hiller et al., vol. 29, Royal Society of Chemistry,
    2023, pp. 278–318, doi:<a href="https://doi.org/10.1039/bk9781839165986-00278">10.1039/bk9781839165986-00278</a>.
  short: F. Wruck, M. Avellaneda Sarrió, M.M. Naqvi, E.J. Koers, K. Till, L. Gross,
    F. Moayed, A. Roland, L.W.H.J. Heling, A. Mashaghi, S.J. Tans, in:, S. Hiller,
    M. Liu, L. He (Eds.), Biophysics of Molecular Chaperones, Royal Society of Chemistry,
    2023, pp. 278–318.
date_created: 2024-01-22T08:07:02Z
date_published: 2023-11-01T00:00:00Z
date_updated: 2024-01-23T12:01:53Z
day: '01'
department:
- _id: MiSi
doi: 10.1039/bk9781839165986-00278
editor:
- first_name: Sebastian
  full_name: Hiller, Sebastian
  last_name: Hiller
- first_name: Maili
  full_name: Liu, Maili
  last_name: Liu
- first_name: Lichun
  full_name: He, Lichun
  last_name: He
intvolume: '        29'
language:
- iso: eng
month: '11'
oa_version: None
page: 278-318
publication: Biophysics of Molecular Chaperones
publication_identifier:
  eisbn:
  - '9781839165993'
  isbn:
  - '9781839162824'
publication_status: published
publisher: Royal Society of Chemistry
quality_controlled: '1'
status: public
title: Probing Single Chaperone Substrates
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2023'
...
---
_id: '12085'
abstract:
- lang: eng
  text: Molecular catch bonds are ubiquitous in biology and essential for processes
    like leucocyte extravasion1 and cellular mechanosensing2. Unlike normal (slip)
    bonds, catch bonds strengthen under tension. The current paradigm is that this
    feature provides ‘strength on demand3’, thus enabling cells to increase rigidity
    under stress1,4,5,6. However, catch bonds are often weaker than slip bonds because
    they have cryptic binding sites that are usually buried7,8. Here we show that
    catch bonds render reconstituted cytoskeletal actin networks stronger than slip
    bonds, even though the individual bonds are weaker. Simulations show that slip
    bonds remain trapped in stress-free areas, whereas weak binding allows catch bonds
    to mitigate crack initiation by moving to high-tension areas. This ‘dissociation
    on demand’ explains how cells combine mechanical strength with the adaptability
    required for shape change, and is relevant to diseases where catch bonding is
    compromised7,9, including focal segmental glomerulosclerosis10 caused by the α-actinin-4
    mutant studied here. We surmise that catch bonds are the key to create life-like
    materials.
acknowledgement: 'We thank M. van Hecke and C. Alkemade for critical reading of the
  manuscript. We thank P. R. ten Wolde, K. Storm, W. Ellenbroek, C. Broedersz, D.
  Brueckner and M. Berger for fruitful discussions. We thank W. Brieher and V. Tang
  from the University of Illinois for the kind gift of purified α-actinin-4 (WT and
  the K255E point mutant) and their plasmids; M. Kuit-Vinkenoog and J. den Haan for
  actin and further purification of α-actinin-4; A. Goutou and I. Isturiz-Petitjean
  for co-sedimentation measurements and V. Sunderlíková for the design, mutagenesis,
  cloning and purifying of the α-actinin-4 constructs used in the single-molecule
  experiments. We gratefully acknowledge financial support from the following sources:
  research program of the Netherlands Organization for Scientific Research (NWO) (S.J.T.,
  A.R. and M.J.A.); ERC Starting Grant (335672-MINICELL) (G.H.K. and Y.M.). ‘BaSyC—Building
  a Synthetic Cell’ Gravitation grant (024.003.019) of the Netherlands Ministry of
  Education, Culture and Science (OCW) and the Netherlands Organisation for Scientific
  Research (G.H.K. and L.B.); and support from the National Institutes of Health (1R01GM126256)
  (T.K. and W.J.).'
article_processing_charge: No
article_type: original
author:
- first_name: Yuval
  full_name: Mulla, Yuval
  last_name: Mulla
- first_name: Mario
  full_name: Avellaneda Sarrió, Mario
  id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E
  last_name: Avellaneda Sarrió
  orcid: 0000-0001-6406-524X
- first_name: Antoine
  full_name: Roland, Antoine
  last_name: Roland
- first_name: Lucia
  full_name: Baldauf, Lucia
  last_name: Baldauf
- first_name: Wonyeong
  full_name: Jung, Wonyeong
  last_name: Jung
- first_name: Taeyoon
  full_name: Kim, Taeyoon
  last_name: Kim
- first_name: Sander J.
  full_name: Tans, Sander J.
  last_name: Tans
- first_name: Gijsje H.
  full_name: Koenderink, Gijsje H.
  last_name: Koenderink
citation:
  ama: Mulla Y, Avellaneda Sarrió M, Roland A, et al. Weak catch bonds make strong
    networks. <i>Nature Materials</i>. 2022;21(9):1019-1023. doi:<a href="https://doi.org/10.1038/s41563-022-01288-0">10.1038/s41563-022-01288-0</a>
  apa: Mulla, Y., Avellaneda Sarrió, M., Roland, A., Baldauf, L., Jung, W., Kim, T.,
    … Koenderink, G. H. (2022). Weak catch bonds make strong networks. <i>Nature Materials</i>.
    Springer Nature. <a href="https://doi.org/10.1038/s41563-022-01288-0">https://doi.org/10.1038/s41563-022-01288-0</a>
  chicago: Mulla, Yuval, Mario Avellaneda Sarrió, Antoine Roland, Lucia Baldauf, Wonyeong
    Jung, Taeyoon Kim, Sander J. Tans, and Gijsje H. Koenderink. “Weak Catch Bonds
    Make Strong Networks.” <i>Nature Materials</i>. Springer Nature, 2022. <a href="https://doi.org/10.1038/s41563-022-01288-0">https://doi.org/10.1038/s41563-022-01288-0</a>.
  ieee: Y. Mulla <i>et al.</i>, “Weak catch bonds make strong networks,” <i>Nature
    Materials</i>, vol. 21, no. 9. Springer Nature, pp. 1019–1023, 2022.
  ista: Mulla Y, Avellaneda Sarrió M, Roland A, Baldauf L, Jung W, Kim T, Tans SJ,
    Koenderink GH. 2022. Weak catch bonds make strong networks. Nature Materials.
    21(9), 1019–1023.
  mla: Mulla, Yuval, et al. “Weak Catch Bonds Make Strong Networks.” <i>Nature Materials</i>,
    vol. 21, no. 9, Springer Nature, 2022, pp. 1019–23, doi:<a href="https://doi.org/10.1038/s41563-022-01288-0">10.1038/s41563-022-01288-0</a>.
  short: Y. Mulla, M. Avellaneda Sarrió, A. Roland, L. Baldauf, W. Jung, T. Kim, S.J.
    Tans, G.H. Koenderink, Nature Materials 21 (2022) 1019–1023.
date_created: 2022-09-11T22:01:57Z
date_published: 2022-09-01T00:00:00Z
date_updated: 2023-08-03T14:08:47Z
day: '01'
department:
- _id: MiSi
doi: 10.1038/s41563-022-01288-0
external_id:
  isi:
  - '000844592000002'
  pmid:
  - '36008604'
intvolume: '        21'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2020.07.27.219618
month: '09'
oa: 1
oa_version: Preprint
page: 1019-1023
pmid: 1
publication: Nature Materials
publication_identifier:
  eissn:
  - 1476-4660
  issn:
  - 1476-1122
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Weak catch bonds make strong networks
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 21
year: '2022'
...