---
_id: '12163'
abstract:
- lang: eng
  text: Small GTPases play essential roles in the organization of eukaryotic cells.
    In recent years, it has become clear that their intracellular functions result
    from intricate biochemical networks of the GTPase and their regulators that dynamically
    bind to a membrane surface. Due to the inherent complexities of their interactions,
    however, revealing the underlying mechanisms of action is often difficult to achieve
    from in vivo studies. This review summarizes in vitro reconstitution approaches
    developed to obtain a better mechanistic understanding of how small GTPase activities
    are regulated in space and time.
acknowledgement: The authors acknowledge support from IST Austria and helpful comments
  from the anonymous reviewers that helped to improve this manuscript. We apologize
  to the authors of primary literature and outstanding research not cited here due
  to space restraints.
article_processing_charge: Yes (via OA deal)
article_type: review
author:
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
- first_name: Albert
  full_name: Auer, Albert
  id: 3018E8C2-F248-11E8-B48F-1D18A9856A87
  last_name: Auer
  orcid: 0000-0002-3580-2906
- first_name: Gabriel
  full_name: Brognara, Gabriel
  id: D96FFDA0-A884-11E9-9968-DC26E6697425
  last_name: Brognara
- first_name: Hanifatul R
  full_name: Budiman, Hanifatul R
  id: 55380f95-15b2-11ec-abd3-aff8e230696b
  last_name: Budiman
- first_name: Lukasz M
  full_name: Kowalski, Lukasz M
  id: e3a512e2-4bbe-11eb-a68a-e3857a7844c2
  last_name: Kowalski
- first_name: Ivana
  full_name: Matijevic, Ivana
  id: 83c17ce3-15b2-11ec-abd3-f486545870bd
  last_name: Matijevic
citation:
  ama: Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. In vitro
    reconstitution of small GTPase regulation. <i>FEBS Letters</i>. 2023;597(6):762-777.
    doi:<a href="https://doi.org/10.1002/1873-3468.14540">10.1002/1873-3468.14540</a>
  apa: Loose, M., Auer, A., Brognara, G., Budiman, H. R., Kowalski, L. M., &#38; Matijevic,
    I. (2023). In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>.
    Wiley. <a href="https://doi.org/10.1002/1873-3468.14540">https://doi.org/10.1002/1873-3468.14540</a>
  chicago: Loose, Martin, Albert Auer, Gabriel Brognara, Hanifatul R Budiman, Lukasz
    M Kowalski, and Ivana Matijevic. “In Vitro Reconstitution of Small GTPase Regulation.”
    <i>FEBS Letters</i>. Wiley, 2023. <a href="https://doi.org/10.1002/1873-3468.14540">https://doi.org/10.1002/1873-3468.14540</a>.
  ieee: M. Loose, A. Auer, G. Brognara, H. R. Budiman, L. M. Kowalski, and I. Matijevic,
    “In vitro reconstitution of small GTPase regulation,” <i>FEBS Letters</i>, vol.
    597, no. 6. Wiley, pp. 762–777, 2023.
  ista: Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. 2023. In
    vitro reconstitution of small GTPase regulation. FEBS Letters. 597(6), 762–777.
  mla: Loose, Martin, et al. “In Vitro Reconstitution of Small GTPase Regulation.”
    <i>FEBS Letters</i>, vol. 597, no. 6, Wiley, 2023, pp. 762–77, doi:<a href="https://doi.org/10.1002/1873-3468.14540">10.1002/1873-3468.14540</a>.
  short: M. Loose, A. Auer, G. Brognara, H.R. Budiman, L.M. Kowalski, I. Matijevic,
    FEBS Letters 597 (2023) 762–777.
date_created: 2023-01-12T12:09:58Z
date_published: 2023-03-01T00:00:00Z
date_updated: 2023-08-16T08:32:29Z
day: '01'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1002/1873-3468.14540
external_id:
  isi:
  - '000891573000001'
  pmid:
  - '36448231'
file:
- access_level: open_access
  checksum: 7492244d3f9c5faa1347ef03f6e5bc84
  content_type: application/pdf
  creator: dernst
  date_created: 2023-08-16T08:31:04Z
  date_updated: 2023-08-16T08:31:04Z
  file_id: '14063'
  file_name: 2023_FEBSLetters_Loose.pdf
  file_size: 3148143
  relation: main_file
  success: 1
file_date_updated: 2023-08-16T08:31:04Z
has_accepted_license: '1'
intvolume: '       597'
isi: 1
issue: '6'
keyword:
- Cell Biology
- Genetics
- Molecular Biology
- Biochemistry
- Structural Biology
- Biophysics
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 762-777
pmid: 1
publication: FEBS Letters
publication_identifier:
  eissn:
  - 1873-3468
  issn:
  - 0014-5793
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro reconstitution of small GTPase regulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 597
year: '2023'
...
---
_id: '15036'
abstract:
- lang: eng
  text: The assembly of a septin filament requires that homologous monomers must distinguish
    between one another in establishing appropriate interfaces with their neighbors.
    To understand this phenomenon at the molecular level, we present the first four
    crystal structures of heterodimeric septin complexes. We describe in detail the
    two distinct types of G-interface present within the octameric particles, which
    must polymerize to form filaments. These are formed between SEPT2 and SEPT6 and
    between SEPT7 and SEPT3, and their description permits an understanding of the
    structural basis for the selectivity necessary for correct filament assembly.
    By replacing SEPT6 by SEPT8 or SEPT11, it is possible to rationalize Kinoshita's
    postulate, which predicts the exchangeability of septins from within a subgroup.
    Switches I and II, which in classical small GTPases provide a mechanism for nucleotide-dependent
    conformational change, have been repurposed in septins to play a fundamental role
    in molecular recognition. Specifically, it is switch I which holds the key to
    discriminating between the two different G-interfaces. Moreover, residues which
    are characteristic for a given subgroup play subtle, but pivotal, roles in guaranteeing
    that the correct interfaces are formed.
article_processing_charge: No
article_type: original
author:
- first_name: Higor Vinícius Dias
  full_name: Rosa, Higor Vinícius Dias
  last_name: Rosa
- first_name: Diego Antonio
  full_name: Leonardo, Diego Antonio
  last_name: Leonardo
- first_name: Gabriel
  full_name: Brognara, Gabriel
  id: D96FFDA0-A884-11E9-9968-DC26E6697425
  last_name: Brognara
- first_name: José
  full_name: Brandão-Neto, José
  last_name: Brandão-Neto
- first_name: Humberto
  full_name: D'Muniz Pereira, Humberto
  last_name: D'Muniz Pereira
- first_name: Ana Paula Ulian
  full_name: Araújo, Ana Paula Ulian
  last_name: Araújo
- first_name: Richard Charles
  full_name: Garratt, Richard Charles
  last_name: Garratt
citation:
  ama: 'Rosa HVD, Leonardo DA, Brognara G, et al. Molecular recognition at septin
    interfaces: The switches hold the key. <i>Journal of Molecular Biology</i>. 2020;432(21):5784-5801.
    doi:<a href="https://doi.org/10.1016/j.jmb.2020.09.001">10.1016/j.jmb.2020.09.001</a>'
  apa: 'Rosa, H. V. D., Leonardo, D. A., Brognara, G., Brandão-Neto, J., D’Muniz Pereira,
    H., Araújo, A. P. U., &#38; Garratt, R. C. (2020). Molecular recognition at septin
    interfaces: The switches hold the key. <i>Journal of Molecular Biology</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.jmb.2020.09.001">https://doi.org/10.1016/j.jmb.2020.09.001</a>'
  chicago: 'Rosa, Higor Vinícius Dias, Diego Antonio Leonardo, Gabriel Brognara, José
    Brandão-Neto, Humberto D’Muniz Pereira, Ana Paula Ulian Araújo, and Richard Charles
    Garratt. “Molecular Recognition at Septin Interfaces: The Switches Hold the Key.”
    <i>Journal of Molecular Biology</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.jmb.2020.09.001">https://doi.org/10.1016/j.jmb.2020.09.001</a>.'
  ieee: 'H. V. D. Rosa <i>et al.</i>, “Molecular recognition at septin interfaces:
    The switches hold the key,” <i>Journal of Molecular Biology</i>, vol. 432, no.
    21. Elsevier, pp. 5784–5801, 2020.'
  ista: 'Rosa HVD, Leonardo DA, Brognara G, Brandão-Neto J, D’Muniz Pereira H, Araújo
    APU, Garratt RC. 2020. Molecular recognition at septin interfaces: The switches
    hold the key. Journal of Molecular Biology. 432(21), 5784–5801.'
  mla: 'Rosa, Higor Vinícius Dias, et al. “Molecular Recognition at Septin Interfaces:
    The Switches Hold the Key.” <i>Journal of Molecular Biology</i>, vol. 432, no.
    21, Elsevier, 2020, pp. 5784–801, doi:<a href="https://doi.org/10.1016/j.jmb.2020.09.001">10.1016/j.jmb.2020.09.001</a>.'
  short: H.V.D. Rosa, D.A. Leonardo, G. Brognara, J. Brandão-Neto, H. D’Muniz Pereira,
    A.P.U. Araújo, R.C. Garratt, Journal of Molecular Biology 432 (2020) 5784–5801.
date_created: 2024-02-28T08:50:34Z
date_published: 2020-10-02T00:00:00Z
date_updated: 2024-02-28T12:37:54Z
day: '02'
department:
- _id: MaLo
doi: 10.1016/j.jmb.2020.09.001
external_id:
  pmid:
  - '32910969'
intvolume: '       432'
issue: '21'
keyword:
- Molecular Biology
- Structural Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.jmb.2020.09.001
month: '10'
oa: 1
oa_version: Published Version
page: 5784-5801
pmid: 1
publication: Journal of Molecular Biology
publication_identifier:
  issn:
  - 0022-2836
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: 'Molecular recognition at septin interfaces: The switches hold the key'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 432
year: '2020'
...