---
_id: '10116'
abstract:
- lang: eng
  text: The ubiquitous Ca2+ sensor calmodulin (CaM) binds and regulates many proteins,
    including ion channels, CaM kinases, and calcineurin, according to Ca2+-CaM levels.
    What regulates neuronal CaM levels, is, however, unclear. CaM-binding transcription
    activators (CAMTAs) are ancient proteins expressed broadly in nervous systems
    and whose loss confers pleiotropic behavioral defects in flies, mice, and humans.
    Using Caenorhabditis elegans and Drosophila, we show that CAMTAs control neuronal
    CaM levels. The behavioral and neuronal Ca2+ signaling defects in mutants lacking
    camt-1, the sole C. elegans CAMTA, can be rescued by supplementing neuronal CaM.
    CAMT-1 binds multiple sites in the CaM promoter and deleting these sites phenocopies
    camt-1. Our data suggest CAMTAs mediate a conserved and general mechanism that
    controls neuronal CaM levels, thereby regulating Ca2+ signaling, physiology, and
    behavior.
acknowledgement: The authors thank the MRC-LMB Flow Cytometry facility and Imaging
  Service for support, the Cancer Research UK Cambridge Institute Genomics Core for
  Next Generation Sequencing, Julie Ahringer and Alex Appert for advice and technical
  help for ChIP-seq experiments, Paula Freire-Pritchett, Tim Stevens, and Gurpreet
  Ghattaoraya for RNA-seq and ChIP-seq analyses, Nikos Chronis for the TN-XL plasmid,
  Hong-Sheng Li and Daisuke Yamamoto for generously sending the tes2 and cro mutants,
  Daria Siekhaus for hosting the fly work, Michaela Misova for technical assistance.
  The authors are very grateful to Salihah Ece Sönmez for teaching us how to dissect,
  mount and stain Drosophila retinae. This work was supported by an Advanced ERC grant
  (269058 ACMO) and a Wellcome Investigator Award (209504/Z/17/Z) to MdB, and an IST
  Plus Fellowship to TV-B (Marie Sklodowska-Curie Agreement no 754411).
article_number: e68238
article_processing_charge: No
article_type: original
author:
- first_name: Thanh
  full_name: Vuong-Brender, Thanh
  id: D389312E-10C4-11EA-ABF4-A4B43DDC885E
  last_name: Vuong-Brender
- first_name: Sean
  full_name: Flynn, Sean
  last_name: Flynn
- first_name: Yvonne
  full_name: Vallis, Yvonne
  id: 05A2795C-31B5-11EA-83A7-7DA23DDC885E
  last_name: Vallis
- first_name: Mario
  full_name: De Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: De Bono
  orcid: 0000-0001-8347-0443
citation:
  ama: Vuong-Brender T, Flynn S, Vallis Y, de Bono M. Neuronal calmodulin levels are
    controlled by CAMTA transcription factors. <i>eLife</i>. 2021;10. doi:<a href="https://doi.org/10.7554/eLife.68238">10.7554/eLife.68238</a>
  apa: Vuong-Brender, T., Flynn, S., Vallis, Y., &#38; de Bono, M. (2021). Neuronal
    calmodulin levels are controlled by CAMTA transcription factors. <i>ELife</i>.
    eLife Sciences Publications. <a href="https://doi.org/10.7554/eLife.68238">https://doi.org/10.7554/eLife.68238</a>
  chicago: Vuong-Brender, Thanh, Sean Flynn, Yvonne Vallis, and Mario de Bono. “Neuronal
    Calmodulin Levels Are Controlled by CAMTA Transcription Factors.” <i>ELife</i>.
    eLife Sciences Publications, 2021. <a href="https://doi.org/10.7554/eLife.68238">https://doi.org/10.7554/eLife.68238</a>.
  ieee: T. Vuong-Brender, S. Flynn, Y. Vallis, and M. de Bono, “Neuronal calmodulin
    levels are controlled by CAMTA transcription factors,” <i>eLife</i>, vol. 10.
    eLife Sciences Publications, 2021.
  ista: Vuong-Brender T, Flynn S, Vallis Y, de Bono M. 2021. Neuronal calmodulin levels
    are controlled by CAMTA transcription factors. eLife. 10, e68238.
  mla: Vuong-Brender, Thanh, et al. “Neuronal Calmodulin Levels Are Controlled by
    CAMTA Transcription Factors.” <i>ELife</i>, vol. 10, e68238, eLife Sciences Publications,
    2021, doi:<a href="https://doi.org/10.7554/eLife.68238">10.7554/eLife.68238</a>.
  short: T. Vuong-Brender, S. Flynn, Y. Vallis, M. de Bono, ELife 10 (2021).
date_created: 2021-10-10T22:01:22Z
date_published: 2021-09-17T00:00:00Z
date_updated: 2023-08-14T07:23:39Z
day: '17'
ddc:
- '610'
department:
- _id: MaDe
doi: 10.7554/eLife.68238
ec_funded: 1
external_id:
  isi:
  - '000695716100001'
  pmid:
  - '34499028'
file:
- access_level: open_access
  checksum: b465e172d2b1f57aa26a2571a085d052
  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-10-11T14:15:07Z
  date_updated: 2021-10-11T14:15:07Z
  file_id: '10122'
  file_name: 2021_eLife_VuongBrender.pdf
  file_size: 1774624
  relation: main_file
  success: 1
file_date_updated: 2021-10-11T14:15:07Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
publication: eLife
publication_identifier:
  eissn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Neuronal calmodulin levels are controlled by CAMTA transcription factors
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...