---
_id: '11373'
abstract:
- lang: eng
  text: The actin-homologue FtsA is essential for E. coli cell division, as it links
    FtsZ filaments in the Z-ring to transmembrane proteins. FtsA is thought to initiate
    cell constriction by switching from an inactive polymeric to an active monomeric
    conformation, which recruits downstream proteins and stabilizes the Z-ring. However,
    direct biochemical evidence for this mechanism is missing. Here, we use reconstitution
    experiments and quantitative fluorescence microscopy to study divisome activation
    in vitro. By comparing wild-type FtsA with FtsA R286W, we find that this hyperactive
    mutant outperforms FtsA WT in replicating FtsZ treadmilling dynamics, FtsZ filament
    stabilization and recruitment of FtsN. We could attribute these differences to
    a faster exchange and denser packing of FtsA R286W below FtsZ filaments. Using
    FRET microscopy, we also find that FtsN binding promotes FtsA self-interaction.
    We propose that in the active divisome FtsA and FtsN exist as a dynamic copolymer
    that follows treadmilling filaments of FtsZ.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We acknowledge members of the Loose laboratory at IST Austria for
  helpful discussions—in particular L. Lindorfer for his assistance with cloning and
  purifications. We thank J. Löwe and T. Nierhaus (MRC-LMB Cambridge, UK) for sharing
  unpublished work and helpful discussions, as well as D. Vavylonis and D. Rutkowski
  (Lehigh University, Bethlehem, PA, USA) and S. Martin (University of Lausanne, Switzerland)
  for sharing their code for FRAP analysis. We are also thankful for the support by
  the Scientific Service Units (SSU) of IST Austria through resources provided by
  the Imaging and Optics Facility (IOF) and the Lab Support Facility (LSF). This work
  was supported by the European Research Council through grant ERC 2015-StG-679239
  and by the Austrian Science Fund (FWF) StandAlone P34607 to M.L. and HFSP LT 000824/2016-L4
  to N.B. For the purpose of open access, we have applied a CC BY public copyright
  licence to any Author Accepted Manuscript version arising from this submission.
article_number: '2635'
article_processing_charge: No
article_type: original
author:
- first_name: Philipp
  full_name: Radler, Philipp
  id: 40136C2A-F248-11E8-B48F-1D18A9856A87
  last_name: Radler
  orcid: '0000-0001-9198-2182 '
- first_name: Natalia S.
  full_name: Baranova, Natalia S.
  id: 38661662-F248-11E8-B48F-1D18A9856A87
  last_name: Baranova
  orcid: 0000-0002-3086-9124
- first_name: Paulo R
  full_name: Dos Santos Caldas, Paulo R
  id: 38FCDB4C-F248-11E8-B48F-1D18A9856A87
  last_name: Dos Santos Caldas
  orcid: 0000-0001-6730-4461
- first_name: Christoph M
  full_name: Sommer, Christoph M
  id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
  last_name: Sommer
  orcid: 0000-0003-1216-9105
- first_name: Maria D
  full_name: Lopez Pelegrin, Maria D
  id: 319AA9CE-F248-11E8-B48F-1D18A9856A87
  last_name: Lopez Pelegrin
- first_name: David
  full_name: Michalik, David
  id: B9577E20-AA38-11E9-AC9A-0930E6697425
  last_name: Michalik
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: Radler P, Baranova NS, Dos Santos Caldas PR, et al. In vitro reconstitution
    of Escherichia coli divisome activation. <i>Nature Communications</i>. 2022;13.
    doi:<a href="https://doi.org/10.1038/s41467-022-30301-y">10.1038/s41467-022-30301-y</a>
  apa: Radler, P., Baranova, N. S., Dos Santos Caldas, P. R., Sommer, C. M., Lopez
    Pelegrin, M. D., Michalik, D., &#38; Loose, M. (2022). In vitro reconstitution
    of Escherichia coli divisome activation. <i>Nature Communications</i>. Springer
    Nature. <a href="https://doi.org/10.1038/s41467-022-30301-y">https://doi.org/10.1038/s41467-022-30301-y</a>
  chicago: Radler, Philipp, Natalia S. Baranova, Paulo R Dos Santos Caldas, Christoph
    M Sommer, Maria D Lopez Pelegrin, David Michalik, and Martin Loose. “In Vitro
    Reconstitution of Escherichia Coli Divisome Activation.” <i>Nature Communications</i>.
    Springer Nature, 2022. <a href="https://doi.org/10.1038/s41467-022-30301-y">https://doi.org/10.1038/s41467-022-30301-y</a>.
  ieee: P. Radler <i>et al.</i>, “In vitro reconstitution of Escherichia coli divisome
    activation,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.
  ista: Radler P, Baranova NS, Dos Santos Caldas PR, Sommer CM, Lopez Pelegrin MD,
    Michalik D, Loose M. 2022. In vitro reconstitution of Escherichia coli divisome
    activation. Nature Communications. 13, 2635.
  mla: Radler, Philipp, et al. “In Vitro Reconstitution of Escherichia Coli Divisome
    Activation.” <i>Nature Communications</i>, vol. 13, 2635, Springer Nature, 2022,
    doi:<a href="https://doi.org/10.1038/s41467-022-30301-y">10.1038/s41467-022-30301-y</a>.
  short: P. Radler, N.S. Baranova, P.R. Dos Santos Caldas, C.M. Sommer, M.D. Lopez
    Pelegrin, D. Michalik, M. Loose, Nature Communications 13 (2022).
date_created: 2022-05-13T09:06:28Z
date_published: 2022-05-12T00:00:00Z
date_updated: 2024-02-21T12:35:18Z
day: '12'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1038/s41467-022-30301-y
ec_funded: 1
external_id:
  isi:
  - '000795171100037'
file:
- access_level: open_access
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  creator: dernst
  date_created: 2022-05-13T09:10:51Z
  date_updated: 2022-05-13T09:10:51Z
  file_id: '11374'
  file_name: 2022_NatureCommunications_Radler.pdf
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file_date_updated: 2022-05-13T09:10:51Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
keyword:
- General Physics and Astronomy
- General Biochemistry
- Genetics and Molecular Biology
- General Chemistry
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 2595697A-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '679239'
  name: Self-Organization of the Bacterial Cell
- _id: fc38323b-9c52-11eb-aca3-ff8afb4a011d
  grant_number: P34607
  name: "Understanding bacterial cell division by in vitro\r\nreconstitution"
publication: Nature Communications
publication_identifier:
  issn:
  - 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.1038/s41467-022-34485-1
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    status: public
  - id: '10934'
    relation: research_data
    status: public
scopus_import: '1'
status: public
title: In vitro reconstitution of Escherichia coli divisome activation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...