---
_id: '13212'
abstract:
- lang: eng
text: Auxin is the major plant hormone regulating growth and development (Friml,
2022). Forward genetic approaches in the model plant Arabidopsis thaliana have
identified major components of auxin signalling and established the canonical
mechanism mediating transcriptional and thus developmental reprogramming. In this
textbook view, TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFBs)
are auxin receptors, which act as F-box subunits determining the substrate specificity
of the Skp1-Cullin1-F box protein (SCF) type E3 ubiquitin ligase complex. Auxin
acts as a “molecular glue” increasing the affinity between TIR1/AFBs and the Aux/IAA
repressors. Subsequently, Aux/IAAs are ubiquitinated and degraded, thus releasing
auxin transcription factors from their repression making them free to mediate
transcription of auxin response genes (Yu et al., 2022). Nonetheless, accumulating
evidence suggests existence of rapid, non-transcriptional responses downstream
of TIR1/AFBs such as auxin-induced cytosolic calcium (Ca2+) transients, plasma
membrane depolarization and apoplast alkalinisation, all converging on the process
of root growth inhibition and root gravitropism (Li et al., 2022). Particularly,
these rapid responses are mostly contributed by predominantly cytosolic AFB1,
while the long-term growth responses are mediated by mainly nuclear TIR1 and AFB2-AFB5
(Li et al., 2021; Prigge et al., 2020; Serre et al., 2021). How AFB1 conducts
auxin-triggered rapid responses and how it is different from TIR1 and AFB2-AFB5
remains elusive. Here, we compare the roles of TIR1 and AFB1 in transcriptional
and rapid responses by modulating their subcellular localization in Arabidopsis
and by testing their ability to mediate transcriptional responses when part of
the minimal auxin circuit reconstituted in yeast.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: We thank all the authors for sharing the published materials. This
research was supported by the Lab Support Facility and the Imaging and Optics Facility
of ISTA. We thank Lukáš Fiedler (ISTA) for critical reading of the manuscript. This
project was funded by the European Research Council Advanced Grant (ETAP-742985).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen H, Li L, Zou M, Qi L, Friml J. Distinct functions of TIR1 and AFB1 receptors
in auxin signalling. Molecular Plant. 2023;16(7):1117-1119. doi:10.1016/j.molp.2023.06.007
apa: Chen, H., Li, L., Zou, M., Qi, L., & Friml, J. (2023). Distinct functions
of TIR1 and AFB1 receptors in auxin signalling. Molecular Plant. Elsevier
. https://doi.org/10.1016/j.molp.2023.06.007
chicago: Chen, Huihuang, Lanxin Li, Minxia Zou, Linlin Qi, and Jiří Friml. “Distinct
Functions of TIR1 and AFB1 Receptors in Auxin Signalling.” Molecular Plant.
Elsevier , 2023. https://doi.org/10.1016/j.molp.2023.06.007.
ieee: H. Chen, L. Li, M. Zou, L. Qi, and J. Friml, “Distinct functions of TIR1 and
AFB1 receptors in auxin signalling.,” Molecular Plant, vol. 16, no. 7.
Elsevier , pp. 1117–1119, 2023.
ista: Chen H, Li L, Zou M, Qi L, Friml J. 2023. Distinct functions of TIR1 and AFB1
receptors in auxin signalling. Molecular Plant. 16(7), 1117–1119.
mla: Chen, Huihuang, et al. “Distinct Functions of TIR1 and AFB1 Receptors in Auxin
Signalling.” Molecular Plant, vol. 16, no. 7, Elsevier , 2023, pp. 1117–19,
doi:10.1016/j.molp.2023.06.007.
short: H. Chen, L. Li, M. Zou, L. Qi, J. Friml, Molecular Plant 16 (2023) 1117–1119.
date_created: 2023-07-12T07:32:46Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2024-01-29T10:38:57Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2023.06.007
ec_funded: 1
external_id:
isi:
- '001044410900001'
pmid:
- '37393433'
file:
- access_level: open_access
checksum: 6012b7e4a2f680ee6c1f84001e2b945f
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T10:37:05Z
date_updated: 2024-01-29T10:37:05Z
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file_name: 2023_MolecularPlant_Chen.pdf
file_size: 1000871
relation: main_file
success: 1
file_date_updated: 2024-01-29T10:37:05Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1117-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Molecular Plant
publication_identifier:
eissn:
- 1674-2052
issn:
- 1752-9867
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct functions of TIR1 and AFB1 receptors in auxin signalling.
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2023'
...
---
_id: '11723'
abstract:
- lang: eng
text: Plant cell growth responds rapidly to various stimuli, adapting architecture
to environmental changes. Two major endogenous signals regulating growth are the
phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs).
Both trigger very rapid cellular responses and also exert long-term effects [Du
et al., Annu. Rev. Plant Biol. 71, 379–402 (2020); Blackburn et al., Plant Physiol.
182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways
converge to regulate growth, remains unknown. Here, using vertical confocal microscopy
combined with a microfluidic chip, we addressed the mechanism of RALF action on
growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana
root growth inhibition and apoplast alkalinization during the initial phase of
the response, and revealed that RALF1 reversibly inhibits primary root growth
through apoplast alkalinization faster than within 1 min. This rapid apoplast
alkalinization was the result of RALF1-induced net H+ influx and was mediated
by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between
RALF1 and the auxin signaling pathways during root growth regulation. The results
showed that RALF-FER signaling triggered auxin signaling with a delay of approximately
1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced
growth inhibition. This biphasic RALF1 action on growth allows plants to respond
rapidly to environmental stimuli and also reprogram growth and development in
the long term.
acknowledgement: We thank Sarah M. Assmann, Kris Vissenberg, and Nadine Paris for
kindly sharing seeds; Matyáš Fendrych for initiating this project and providing
constant support; Lukas Fiedler for revising the manuscript; and Huibin Han and
Arseny Savin for contributing to genotyping. This work was supported by the Austrian
Science Fund (FWF) I 3630-B25 (to J.F.) and the Doctoral Fellowship Progrmme of
the Austrian Academy of Sciences (to L.L.) We also acknowledge Taif University Researchers
Supporting Project TURSP-HC2021/02 and funding “Plants as a tool for sustainable
global development (no. CZ.02.1.01/0.0/0.0/16_019/0000827).”
article_number: e2121058119
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Saqer S.
full_name: Alotaibi, Saqer S.
last_name: Alotaibi
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Chen H, Alotaibi SS, et al. RALF1 peptide triggers biphasic root growth
inhibition upstream of auxin biosynthesis. Proceedings of the National Academy
of Sciences. 2022;119(31). doi:10.1073/pnas.2121058119
apa: Li, L., Chen, H., Alotaibi, S. S., Pěnčík, A., Adamowski, M., Novák, O., &
Friml, J. (2022). RALF1 peptide triggers biphasic root growth inhibition upstream
of auxin biosynthesis. Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2121058119
chicago: Li, Lanxin, Huihuang Chen, Saqer S. Alotaibi, Aleš Pěnčík, Maciek Adamowski,
Ondřej Novák, and Jiří Friml. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2121058119.
ieee: L. Li et al., “RALF1 peptide triggers biphasic root growth inhibition
upstream of auxin biosynthesis,” Proceedings of the National Academy of Sciences,
vol. 119, no. 31. Proceedings of the National Academy of Sciences, 2022.
ista: Li L, Chen H, Alotaibi SS, Pěnčík A, Adamowski M, Novák O, Friml J. 2022.
RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
Proceedings of the National Academy of Sciences. 119(31), e2121058119.
mla: Li, Lanxin, et al. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences,
vol. 119, no. 31, e2121058119, Proceedings of the National Academy of Sciences,
2022, doi:10.1073/pnas.2121058119.
short: L. Li, H. Chen, S.S. Alotaibi, A. Pěnčík, M. Adamowski, O. Novák, J. Friml,
Proceedings of the National Academy of Sciences 119 (2022).
date_created: 2022-08-04T20:06:49Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2024-10-29T10:12:30Z
day: '25'
ddc:
- '580'
department:
- _id: GradSch
- _id: JiFr
doi: 10.1073/pnas.2121058119
external_id:
isi:
- '000881496900002'
pmid:
- '35878023'
file:
- access_level: open_access
checksum: ae6f19b0d9efba6687f9e4dc1bab1d6e
content_type: application/pdf
creator: dernst
date_created: 2022-08-08T07:42:09Z
date_updated: 2022-08-08T07:42:09Z
file_id: '11747'
file_name: 2022_PNAS_Li.pdf
file_size: 2506262
relation: main_file
success: 1
file_date_updated: 2022-08-08T07:42:09Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '31'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '12144'
abstract:
- lang: eng
text: The phytohormone auxin is the major coordinative signal in plant development1,
mediating transcriptional reprogramming by a well-established canonical signalling
pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin
receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin,
they associate with Aux/IAA transcriptional repressors and target them for degradation
via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an
additional function of TIR1/AFB receptors across land plants. Auxin, together
with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC
motif of the TIR1 C-terminal region, all of which abolish the AC activity, each
render TIR1 ineffective in mediating gravitropism and sustained auxin-induced
root growth inhibition, and also affect auxin-induced transcriptional regulation.
These results highlight the importance of TIR1/AFB AC activity in canonical auxin
signalling. They also identify a unique phytohormone receptor cassette combining
F-box and AC motifs, and the role of cAMP as a second messenger in plants.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: This research was supported by the Lab Support Facility (LSF) and
the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions
and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was
funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N.
acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported
by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in
Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future
Leaders Fellowship grant number MR/T020652/1.
article_processing_charge: No
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Mateusz
full_name: Kwiatkowski, Mateusz
last_name: Kwiatkowski
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Charo I.
full_name: del Genio, Charo I.
last_name: del Genio
- first_name: Martin F.
full_name: Kubeš, Martin F.
last_name: Kubeš
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Krzysztof
full_name: Jaworski, Krzysztof
last_name: Jaworski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Kwiatkowski M, Chen H, et al. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 2022;611(7934):133-138. doi:10.1038/s41586-022-05369-7
apa: Qi, L., Kwiatkowski, M., Chen, H., Hörmayer, L., Sinclair, S. A., Zou, M.,
… Friml, J. (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in
plants. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05369-7
chicago: Qi, Linlin, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hörmayer, Scott A
Sinclair, Minxia Zou, Charo I. del Genio, et al. “Adenylate Cyclase Activity of
TIR1/AFB Auxin Receptors in Plants.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05369-7.
ieee: L. Qi et al., “Adenylate cyclase activity of TIR1/AFB auxin receptors
in plants,” Nature, vol. 611, no. 7934. Springer Nature, pp. 133–138, 2022.
ista: Qi L, Kwiatkowski M, Chen H, Hörmayer L, Sinclair SA, Zou M, del Genio CI,
Kubeš MF, Napier R, Jaworski K, Friml J. 2022. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 611(7934), 133–138.
mla: Qi, Linlin, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors
in Plants.” Nature, vol. 611, no. 7934, Springer Nature, 2022, pp. 133–38,
doi:10.1038/s41586-022-05369-7.
short: L. Qi, M. Kwiatkowski, H. Chen, L. Hörmayer, S.A. Sinclair, M. Zou, C.I.
del Genio, M.F. Kubeš, R. Napier, K. Jaworski, J. Friml, Nature 611 (2022) 133–138.
date_created: 2023-01-12T12:06:05Z
date_published: 2022-11-03T00:00:00Z
date_updated: 2023-10-03T11:04:53Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05369-7
ec_funded: 1
external_id:
isi:
- '000875061600013'
pmid:
- '36289340'
intvolume: ' 611'
isi: 1
issue: '7934'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://wrap.warwick.ac.uk/168325/1/WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf
month: '11'
oa: 1
oa_version: Submitted Version
page: 133-138
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 611
year: '2022'
...