---
_id: '13136'
abstract:
- lang: eng
  text: Despite its fundamental importance for development, the question of how organs
    achieve their correct size and shape is poorly understood. This complex process
    requires coordination between the generation of cell mass and the morphogenetic
    mechanisms that sculpt tissues. These processes are regulated by morphogen signalling
    pathways and mechanical forces. Yet, in many systems, it is unclear how biochemical
    and mechanical signalling are quantitatively interpreted to determine the behaviours
    of individual cells and how they contribute to growth and morphogenesis at the
    tissue scale. In this review, we discuss the development of the vertebrate neural
    tube and somites as an example of the state of knowledge, as well as the challenges
    in understanding the mechanisms of tissue size control in vertebrate organogenesis.
    We highlight how the recent advances in stem cell differentiation and organoid
    approaches can be harnessed to provide new insights into this question.
acknowledgement: 'We thank J. Briscoe for comments on the manuscript. Work in the
  AK lab is supported by ISTA, the European Research Council under Horizon Europe:
  grant 101044579, and Austrian Science Fund (FWF): F78 (Stem Cell Modulation). SR
  is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship
  SC19-011.'
article_number: '100459'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Stefanie
  full_name: Rus, Stefanie
  id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
  last_name: Rus
  orcid: 0000-0001-8703-1093
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: Minchington T, Rus S, Kicheva A. Control of tissue dimensions in the developing
    neural tube and somites. <i>Current Opinion in Systems Biology</i>. 2023;35. doi:<a
    href="https://doi.org/10.1016/j.coisb.2023.100459">10.1016/j.coisb.2023.100459</a>
  apa: Minchington, T., Rus, S., &#38; Kicheva, A. (2023). Control of tissue dimensions
    in the developing neural tube and somites. <i>Current Opinion in Systems Biology</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.coisb.2023.100459">https://doi.org/10.1016/j.coisb.2023.100459</a>
  chicago: Minchington, Thomas, Stefanie Rus, and Anna Kicheva. “Control of Tissue
    Dimensions in the Developing Neural Tube and Somites.” <i>Current Opinion in Systems
    Biology</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.coisb.2023.100459">https://doi.org/10.1016/j.coisb.2023.100459</a>.
  ieee: T. Minchington, S. Rus, and A. Kicheva, “Control of tissue dimensions in the
    developing neural tube and somites,” <i>Current Opinion in Systems Biology</i>,
    vol. 35. Elsevier, 2023.
  ista: Minchington T, Rus S, Kicheva A. 2023. Control of tissue dimensions in the
    developing neural tube and somites. Current Opinion in Systems Biology. 35, 100459.
  mla: Minchington, Thomas, et al. “Control of Tissue Dimensions in the Developing
    Neural Tube and Somites.” <i>Current Opinion in Systems Biology</i>, vol. 35,
    100459, Elsevier, 2023, doi:<a href="https://doi.org/10.1016/j.coisb.2023.100459">10.1016/j.coisb.2023.100459</a>.
  short: T. Minchington, S. Rus, A. Kicheva, Current Opinion in Systems Biology 35
    (2023).
date_created: 2023-06-18T22:00:46Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2024-01-29T11:07:47Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1016/j.coisb.2023.100459
file:
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  creator: dernst
  date_created: 2024-01-29T11:06:45Z
  date_updated: 2024-01-29T11:06:45Z
  file_id: '14896'
  file_name: 2023_CurrOpSystBioloy_Minchington.pdf
  file_size: 598842
  relation: main_file
  success: 1
file_date_updated: 2024-01-29T11:06:45Z
has_accepted_license: '1'
intvolume: '        35'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
  grant_number: '101044579'
  name: Mechanisms of tissue size regulation in spinal cord development
- _id: 059DF620-7A3F-11EA-A408-12923DDC885E
  grant_number: F07802
  name: Morphogen control of growth and pattern in the spinal cord
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
  grant_number: SC19-011
  name: The regulatory logic of pattern formation in the vertebrate dorsal neural
    tube
publication: Current Opinion in Systems Biology
publication_identifier:
  eissn:
  - 2452-3100
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Control of tissue dimensions in the developing neural tube and somites
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 35
year: '2023'
...
---
_id: '12245'
abstract:
- lang: eng
  text: MicroRNAs (miRs) have an important role in tuning dynamic gene expression.
    However, the mechanism by which they are quantitatively controlled is unknown.
    We show that the amount of mature miR-9, a key regulator of neuronal development,
    increases during zebrafish neurogenesis in a sharp stepwise manner. We characterize
    the spatiotemporal profile of seven distinct microRNA primary transcripts (pri-mir)-9s
    that produce the same mature miR-9 and show that they are sequentially expressed
    during hindbrain neurogenesis. Expression of late-onset pri-mir-9-1 is added on
    to, rather than replacing, the expression of early onset pri-mir-9-4 and -9-5
    in single cells. CRISPR/Cas9 mutation of the late-onset pri-mir-9-1 prevents the
    developmental increase of mature miR-9, reduces late neuronal differentiation
    and fails to downregulate Her6 at late stages. Mathematical modelling shows that
    an adaptive network containing Her6 is insensitive to linear increases in miR-9
    but responds to stepwise increases of miR-9. We suggest that a sharp stepwise
    increase of mature miR-9 is created by sequential and additive temporal activation
    of distinct loci. This may be a strategy to overcome adaptation and facilitate
    a transition of Her6 to a new dynamic regime or steady state.
acknowledgement: "We are grateful to Dr Tom Pettini for the advice on smiFISH technique
  and Dr Laure Bally-Cuif for sharing plasmids. The authors also thank the Biological
  Services Facility, Bioimaging and Systems Microscopy Facilities of the University
  of Manchester for technical support.\r\nThis work was supported by a Wellcome Trust
  Senior Research Fellowship (090868/Z/09/Z) and a Wellcome Trust Investigator Award
  (224394/Z/21/Z) to N.P. and a Medical Research Council Career Development Award
  to C.S.M. (MR/V032534/1). J.B. was supported by a Wellcome Trust Four-Year PhD Studentship
  in Basic Science (219992/Z/19/Z). Open Access funding provided by The University
  of Manchester. Deposited in PMC for immediate release."
article_number: dev200474
article_processing_charge: No
article_type: original
author:
- first_name: Ximena
  full_name: Soto, Ximena
  last_name: Soto
- first_name: Joshua
  full_name: Burton, Joshua
  last_name: Burton
- first_name: Cerys S.
  full_name: Manning, Cerys S.
  last_name: Manning
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Robert
  full_name: Lea, Robert
  last_name: Lea
- first_name: Jessica
  full_name: Lee, Jessica
  last_name: Lee
- first_name: Jochen
  full_name: Kursawe, Jochen
  last_name: Kursawe
- first_name: Magnus
  full_name: Rattray, Magnus
  last_name: Rattray
- first_name: Nancy
  full_name: Papalopulu, Nancy
  last_name: Papalopulu
citation:
  ama: Soto X, Burton J, Manning CS, et al. Sequential and additive expression of
    miR-9 precursors control timing of neurogenesis. <i>Development</i>. 2022;149(19).
    doi:<a href="https://doi.org/10.1242/dev.200474">10.1242/dev.200474</a>
  apa: Soto, X., Burton, J., Manning, C. S., Minchington, T., Lea, R., Lee, J., …
    Papalopulu, N. (2022). Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis. <i>Development</i>. The Company of Biologists.
    <a href="https://doi.org/10.1242/dev.200474">https://doi.org/10.1242/dev.200474</a>
  chicago: Soto, Ximena, Joshua Burton, Cerys S. Manning, Thomas Minchington, Robert
    Lea, Jessica Lee, Jochen Kursawe, Magnus Rattray, and Nancy Papalopulu. “Sequential
    and Additive Expression of MiR-9 Precursors Control Timing of Neurogenesis.” <i>Development</i>.
    The Company of Biologists, 2022. <a href="https://doi.org/10.1242/dev.200474">https://doi.org/10.1242/dev.200474</a>.
  ieee: X. Soto <i>et al.</i>, “Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis,” <i>Development</i>, vol. 149, no. 19. The Company
    of Biologists, 2022.
  ista: Soto X, Burton J, Manning CS, Minchington T, Lea R, Lee J, Kursawe J, Rattray
    M, Papalopulu N. 2022. Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis. Development. 149(19), dev200474.
  mla: Soto, Ximena, et al. “Sequential and Additive Expression of MiR-9 Precursors
    Control Timing of Neurogenesis.” <i>Development</i>, vol. 149, no. 19, dev200474,
    The Company of Biologists, 2022, doi:<a href="https://doi.org/10.1242/dev.200474">10.1242/dev.200474</a>.
  short: X. Soto, J. Burton, C.S. Manning, T. Minchington, R. Lea, J. Lee, J. Kursawe,
    M. Rattray, N. Papalopulu, Development 149 (2022).
date_created: 2023-01-16T09:53:17Z
date_published: 2022-10-01T00:00:00Z
date_updated: 2023-08-04T09:41:08Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.200474
external_id:
  isi:
  - '000918161000003'
  pmid:
  - '36189829'
file:
- access_level: open_access
  checksum: d7c29b74e9e4032308228cc704a30e88
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-30T08:35:44Z
  date_updated: 2023-01-30T08:35:44Z
  file_id: '12438'
  file_name: 2022_Development_Soto.pdf
  file_size: 9348839
  relation: main_file
  success: 1
file_date_updated: 2023-01-30T08:35:44Z
has_accepted_license: '1'
intvolume: '       149'
isi: 1
issue: '19'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
  eissn:
  - 1477-9129
  issn:
  - 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: ' https://github.com/burtonjosh/StepwiseMir9'
scopus_import: '1'
status: public
title: Sequential and additive expression of miR-9 precursors control timing of neurogenesis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 149
year: '2022'
...