---
_id: '9603'
abstract:
- lang: eng
  text: Mosaic analysis with double markers (MADM) offers one approach to visualize
    and concomitantly manipulate genetically defined cells in mice with single-cell
    resolution. MADM applications include the analysis of lineage, single-cell morphology
    and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous
    gene functions in vivo in health and disease. Yet, MADM can only be applied to
    <25% of all mouse genes on select chromosomes to date. To overcome this limitation,
    we generate transgenic mice with knocked-in MADM cassettes near the centromeres
    of all 19 autosomes and validate their use across organs. With this resource,
    >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic
    analysis. Beyond a proof of principle, we apply our MADM library to systematically
    trace sister chromatid segregation in distinct mitotic cell lineages. We find
    striking chromosome-specific biases in segregation patterns, reflecting a putative
    mechanism for the asymmetric segregation of genetic determinants in somatic stem
    cell division.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: We thank the Bioimaging, Life Science, and Pre-Clinical Facilities
  at IST Austria; M.P. Postiglione, C. Simbriger, K. Valoskova, C. Schwayer, T. Hussain,
  M. Pieber, and V. Wimmer for initial experiments, technical support, and/or assistance;
  R. Shigemoto for sharing iv (Dnah11 mutant) mice; and M. Sixt and all members of
  the Hippenmeyer lab for discussion. This work was supported by National Institutes
  of Health grants ( R01-NS050580 to L.L. and F32MH096361 to L.A.S.). L.L. is an investigator
  of HHMI. N.A. received support from FWF Firnberg-Programm ( T 1031 ). A.H.H. is
  a recipient of a DOC Fellowship (24812) of the Austrian Academy of Sciences . This
  work also received support from IST Austria institutional funds , FWF SFB F78 to
  S.H., the People Programme (Marie Curie Actions) of the European Union’s Seventh
  Framework Programme ( FP7/2007-2013 ) under REA grant agreement no 618444 to S.H.,
  and the European Research Council (ERC) under the European Union’s Horizon 2020
  Research and Innovation Programme (grant agreement no. 725780 LinPro ) to S.H.
article_number: '109274'
article_processing_charge: No
article_type: original
author:
- first_name: Ximena
  full_name: Contreras, Ximena
  id: 475990FE-F248-11E8-B48F-1D18A9856A87
  last_name: Contreras
- first_name: Nicole
  full_name: Amberg, Nicole
  id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
  last_name: Amberg
  orcid: 0000-0002-3183-8207
- first_name: Amarbayasgalan
  full_name: Davaatseren, Amarbayasgalan
  id: 70ADC922-B424-11E9-99E3-BA18E6697425
  last_name: Davaatseren
- first_name: Andi H
  full_name: Hansen, Andi H
  id: 38853E16-F248-11E8-B48F-1D18A9856A87
  last_name: Hansen
- first_name: Johanna
  full_name: Sonntag, Johanna
  id: 32FE7D7C-F248-11E8-B48F-1D18A9856A87
  last_name: Sonntag
- first_name: Lill
  full_name: Andersen, Lill
  last_name: Andersen
- first_name: Tina
  full_name: Bernthaler, Tina
  last_name: Bernthaler
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Anna-Magdalena
  full_name: Heger, Anna-Magdalena
  id: 4B76FFD2-F248-11E8-B48F-1D18A9856A87
  last_name: Heger
- first_name: Randy L.
  full_name: Johnson, Randy L.
  last_name: Johnson
- first_name: Lindsay A.
  full_name: Schwarz, Lindsay A.
  last_name: Schwarz
- first_name: Liqun
  full_name: Luo, Liqun
  last_name: Luo
- first_name: Thomas
  full_name: Rülicke, Thomas
  last_name: Rülicke
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Contreras X, Amberg N, Davaatseren A, et al. A genome-wide library of MADM
    mice for single-cell genetic mosaic analysis. <i>Cell Reports</i>. 2021;35(12).
    doi:<a href="https://doi.org/10.1016/j.celrep.2021.109274">10.1016/j.celrep.2021.109274</a>
  apa: Contreras, X., Amberg, N., Davaatseren, A., Hansen, A. H., Sonntag, J., Andersen,
    L., … Hippenmeyer, S. (2021). A genome-wide library of MADM mice for single-cell
    genetic mosaic analysis. <i>Cell Reports</i>. Cell Press. <a href="https://doi.org/10.1016/j.celrep.2021.109274">https://doi.org/10.1016/j.celrep.2021.109274</a>
  chicago: Contreras, Ximena, Nicole Amberg, Amarbayasgalan Davaatseren, Andi H Hansen,
    Johanna Sonntag, Lill Andersen, Tina Bernthaler, et al. “A Genome-Wide Library
    of MADM Mice for Single-Cell Genetic Mosaic Analysis.” <i>Cell Reports</i>. Cell
    Press, 2021. <a href="https://doi.org/10.1016/j.celrep.2021.109274">https://doi.org/10.1016/j.celrep.2021.109274</a>.
  ieee: X. Contreras <i>et al.</i>, “A genome-wide library of MADM mice for single-cell
    genetic mosaic analysis,” <i>Cell Reports</i>, vol. 35, no. 12. Cell Press, 2021.
  ista: Contreras X, Amberg N, Davaatseren A, Hansen AH, Sonntag J, Andersen L, Bernthaler
    T, Streicher C, Heger A-M, Johnson RL, Schwarz LA, Luo L, Rülicke T, Hippenmeyer
    S. 2021. A genome-wide library of MADM mice for single-cell genetic mosaic analysis.
    Cell Reports. 35(12), 109274.
  mla: Contreras, Ximena, et al. “A Genome-Wide Library of MADM Mice for Single-Cell
    Genetic Mosaic Analysis.” <i>Cell Reports</i>, vol. 35, no. 12, 109274, Cell Press,
    2021, doi:<a href="https://doi.org/10.1016/j.celrep.2021.109274">10.1016/j.celrep.2021.109274</a>.
  short: X. Contreras, N. Amberg, A. Davaatseren, A.H. Hansen, J. Sonntag, L. Andersen,
    T. Bernthaler, C. Streicher, A.-M. Heger, R.L. Johnson, L.A. Schwarz, L. Luo,
    T. Rülicke, S. Hippenmeyer, Cell Reports 35 (2021).
date_created: 2021-06-27T22:01:48Z
date_published: 2021-06-22T00:00:00Z
date_updated: 2023-08-10T13:55:00Z
day: '22'
ddc:
- '570'
department:
- _id: SiHi
- _id: LoSw
- _id: PreCl
doi: 10.1016/j.celrep.2021.109274
ec_funded: 1
external_id:
  isi:
  - '000664463600016'
file:
- access_level: open_access
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  creator: asandaue
  date_created: 2021-06-28T14:06:24Z
  date_updated: 2021-06-28T14:06:24Z
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file_date_updated: 2021-06-28T14:06:24Z
has_accepted_license: '1'
intvolume: '        35'
isi: 1
issue: '12'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 2625A13E-B435-11E9-9278-68D0E5697425
  grant_number: '24812'
  name: Molecular Mechanisms of Radial Neuronal Migration
- _id: 25D61E48-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '618444'
  name: Molecular Mechanisms of Cerebral Cortex Development
- _id: 260018B0-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '725780'
  name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: Cell Reports
publication_identifier:
  eissn:
  - '22111247'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/boost-for-mouse-genetic-analysis/
scopus_import: '1'
status: public
title: A genome-wide library of MADM mice for single-cell genetic mosaic analysis
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 35
year: '2021'
...