[{"title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","oa_version":"Preprint","article_processing_charge":"No","day":"16","doi":"10.1101/2024.02.15.580289","author":[{"full_name":"Jaeger, Eliza C.B.","last_name":"Jaeger","first_name":"Eliza C.B."},{"last_name":"Vijatovic","full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58","first_name":"David"},{"first_name":"Astrid","full_name":"Deryckere, Astrid","last_name":"Deryckere"},{"first_name":"Nikol","full_name":"Zorin, Nikol","last_name":"Zorin"},{"first_name":"Akemi L.","full_name":"Nguyen, Akemi L.","last_name":"Nguyen"},{"full_name":"Ivanian, Georgiy","id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","last_name":"Ivanian","first_name":"Georgiy"},{"first_name":"Jamie","last_name":"Woych","full_name":"Woych, Jamie"},{"last_name":"Arnold","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f","full_name":"Arnold, Rebecca C","first_name":"Rebecca C"},{"first_name":"Alonso","last_name":"Ortega Gurrola","full_name":"Ortega Gurrola, Alonso"},{"first_name":"Arik","full_name":"Shvartsman, Arik","last_name":"Shvartsman"},{"id":"a9492887-8972-11ed-ae7b-bfae10998254","full_name":"Barbieri, Francesca","last_name":"Barbieri","first_name":"Francesca"},{"first_name":"Florina-Alexandra","last_name":"Toma","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5","full_name":"Toma, Florina-Alexandra"},{"first_name":"Gary J.","last_name":"Gorbsky","full_name":"Gorbsky, Gary J."},{"full_name":"Horb, Marko E.","last_name":"Horb","first_name":"Marko E."},{"last_name":"Cline","full_name":"Cline, Hollis T.","first_name":"Hollis T."},{"full_name":"Shay, Timothy F.","last_name":"Shay","first_name":"Timothy F."},{"last_name":"Kelley","full_name":"Kelley, Darcy B.","first_name":"Darcy B."},{"first_name":"Ayako","last_name":"Yamaguchi","full_name":"Yamaguchi, Ayako"},{"full_name":"Shein-Idelson, Mark","last_name":"Shein-Idelson","first_name":"Mark"},{"last_name":"Tosches","full_name":"Tosches, Maria Antonietta","first_name":"Maria Antonietta"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}],"date_created":"2024-02-20T09:20:32Z","type":"preprint","_id":"15016","date_updated":"2024-02-20T09:34:25Z","abstract":[{"lang":"eng","text":"The development, evolution, and function of the vertebrate central nervous system (CNS) can be best studied using diverse model organisms. Amphibians, with their unique phylogenetic position at the transition between aquatic and terrestrial lifestyles, are valuable for understanding the origin and evolution of the tetrapod brain and spinal cord. Their metamorphic developmental transitions and unique regenerative abilities also facilitate the discovery of mechanisms for neural circuit remodeling and replacement. The genetic toolkit for amphibians, however, remains limited, with only a few species having sequenced genomes and a small number of transgenic lines available. In mammals, recombinant adeno-associated viral vectors (AAVs) have become a powerful alternative to genome modification for visualizing and perturbing the nervous system. AAVs are DNA viruses that enable neuronal transduction in both developing and adult animals with low toxicity and spatial, temporal, and cell-type specificity. However, AAVs have never been shown to transduce amphibian cells efficiently. To bridge this gap, we established a simple, scalable, and robust strategy to screen AAV serotypes in three distantly-related amphibian species: the frogs Xenopus laevis and Pelophylax bedriagae, and the salamander Pleurodeles waltl, in both developing larval tadpoles and post-metamorphic animals. For each species, we successfully identified at least two AAV serotypes capable of infecting the CNS; however, no pan-amphibian serotype was identified, indicating rapid evolution of AAV tropism. In addition, we developed an AAV-based strategy that targets isochronic cohorts of developing neurons – a critical tool for parsing neural circuit assembly. Finally, to enable visualization and manipulation of neural circuits, we identified AAV variants for retrograde tracing of neuronal projections in adult animals. Our findings expand the toolkit for amphibians to include AAVs, establish a generalizable workflow for AAV screening in non-canonical research organisms, generate testable hypotheses for the evolution of AAV tropism, and lay the foundation for modern cross-species comparisons of vertebrate CNS development, function, and evolution. "}],"publication_status":"submitted","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.15.580289"}],"month":"02","year":"2024","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"publication":"bioRxiv","language":[{"iso":"eng"}],"status":"public","project":[{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Entwicklung und Funktion der V1 Interneuronen vom Schwimmen zum Laufen während der Metamorphose von Xenopus"},{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We would like to extend our thanks to members of the Sweeney, Tosches, Shein-Idelson,\r\nYamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion and support.\r\nWe additionally thank the Beckman Institute Clover Center and Viviana Gradinaru (Caltech),\r\nKimberly Ritola (UNC NeuroTools), Flavia Gama Gomez Leite (ISTA Viral Core), and Hüseyin\r\nCihan Önal (Shigemoto Group, ISTA) for their consultation and assistance regarding AAVs, as\r\nwell as Andras Simon and Alberto Joven for feedback and discussions on AAVs in Pleurodeles.\r\nTo do these experiments, we have also benefited from the tremendous support of our animal care and imaging facilities at our respective institutions, as well as the amphibian stock centers\r\n(National Xenopus Resource Center, European Xenopus Resource Center, Xenopus Express)\r\nand our funding sources: U.S. National Science Foundation (NSF) Grant Number IOS 2110086\r\n(D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); United States-Israel Binational Science Foundation\r\n(BSF) Grant Number 2020702 (M.S.-I.); NSF Award Number 1645105 (G.J.G., M.E.H.); FTI\r\nStrategy Lower Austria Dissertation Grant Number FTI21-D-046 (D.V.); Horizon Europe ERC\r\nStarting Grant Number 101041551 (L.B.S.); NIH grant number R35GM146973 (M.A.T.); Rita Allen\r\nFoundation award number GA_032522_FE (M.A.T.); European Molecular Biology Organization\r\nLong-Term Fellowship ALTF 874-2021 (A.D.); National Science Foundation Graduate Research\r\nFellowship DGE 2036197 (E.C.J.B.); NIH grant number P40OD010997 (M.E.H).","date_published":"2024-02-16T00:00:00Z","citation":{"ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. Adeno-associated viral tools to trace neural development and connectivity across amphibians. bioRxiv, <a href=\"https://doi.org/10.1101/2024.02.15.580289\">10.1101/2024.02.15.580289</a>.","chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.02.15.580289\">https://doi.org/10.1101/2024.02.15.580289</a>.","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (n.d.). Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.02.15.580289\">https://doi.org/10.1101/2024.02.15.580289</a>","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.02.15.580289\">10.1101/2024.02.15.580289</a>.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.02.15.580289\">10.1101/2024.02.15.580289</a>","ieee":"E. C. B. Jaeger <i>et al.</i>, “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” <i>bioRxiv</i>. .","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, BioRxiv (n.d.)."}},{"isi":1,"year":"2023","external_id":{"isi":["000984606200001"],"pmid":["37180760"]},"project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"}],"publication":"Frontiers in Neural Circuits","status":"public","pmid":1,"date_published":"2023-04-26T00:00:00Z","acknowledgement":"This work was supported by the ERC Starting grant, ERC-2021-STG #101041551.","doi":"10.3389/fncir.2023.1146449","article_processing_charge":"Yes","publisher":"Frontiers","date_updated":"2024-01-31T10:15:53Z","_id":"13097","type":"journal_article","ddc":["570"],"quality_controlled":"1","month":"04","department":[{"_id":"LoSw"}],"article_number":"1146449","file":[{"file_id":"14729","creator":"dernst","date_updated":"2024-01-03T13:33:21Z","file_size":6667157,"date_created":"2024-01-03T13:33:21Z","checksum":"7efd06de284a28e91e97127611a9c3fd","relation":"main_file","content_type":"application/pdf","access_level":"open_access","file_name":"2023_FrontiersNeuralCircuits_Wilson.pdf","success":1}],"oa":1,"language":[{"iso":"eng"}],"citation":{"ieee":"A. C. Wilson and L. B. Sweeney, “Spinal cords: Symphonies of interneurons across species,” <i>Frontiers in Neural Circuits</i>, vol. 17. Frontiers, 2023.","short":"A.C. Wilson, L.B. Sweeney, Frontiers in Neural Circuits 17 (2023).","ama":"Wilson AC, Sweeney LB. Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. 2023;17. doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>","apa":"Wilson, A. C., &#38; Sweeney, L. B. (2023). Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. Frontiers. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>","mla":"Wilson, Alexia C., and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>, vol. 17, 1146449, Frontiers, 2023, doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>.","ista":"Wilson AC, Sweeney LB. 2023. Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. 17, 1146449.","chicago":"Wilson, Alexia C, and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>. Frontiers, 2023. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Alexia C","id":"5230e794-15b2-11ec-abd3-e2d5335ebd1d","full_name":"Wilson, Alexia C","last_name":"Wilson"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}],"day":"26","scopus_import":"1","title":"Spinal cords: Symphonies of interneurons across species","oa_version":"Published Version","volume":17,"article_type":"original","date_created":"2023-05-28T22:01:04Z","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"Vertebrate movement is orchestrated by spinal inter- and motor neurons that, together with sensory and cognitive input, produce dynamic motor behaviors. These behaviors vary from the simple undulatory swimming of fish and larval aquatic species to the highly coordinated running, reaching and grasping of mice, humans and other mammals. This variation raises the fundamental question of how spinal circuits have changed in register with motor behavior. In simple, undulatory fish, exemplified by the lamprey, two broad classes of interneurons shape motor neuron output: ipsilateral-projecting excitatory neurons, and commissural-projecting inhibitory neurons. An additional class of ipsilateral inhibitory neurons is required to generate escape swim behavior in larval zebrafish and tadpoles. In limbed vertebrates, a more complex spinal neuron composition is observed. In this review, we provide evidence that movement elaboration correlates with an increase and specialization of these three basic interneuron types into molecularly, anatomically, and functionally distinct subpopulations. We summarize recent work linking neuron types to movement-pattern generation across fish, amphibians, reptiles, birds and mammals.","lang":"eng"}],"intvolume":"        17","publication_status":"published","publication_identifier":{"issn":["1662-5110"]},"file_date_updated":"2024-01-03T13:33:21Z"},{"author":[{"first_name":"Alina","full_name":"Salamatina, Alina","last_name":"Salamatina"},{"last_name":"Yang","full_name":"Yang, Jerry H","first_name":"Jerry H"},{"last_name":"Brenner-Morton","full_name":"Brenner-Morton, Susan","first_name":"Susan"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B ","first_name":"Jay B "},{"first_name":"Linjing","full_name":"Fang, Linjing","last_name":"Fang"},{"last_name":"Kintner","full_name":"Kintner, Christopher R","first_name":"Christopher R"},{"last_name":"Jessell","full_name":"Jessell, Thomas M","first_name":"Thomas M"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney"}],"day":"01","scopus_import":"1","oa_version":"Published Version","title":"Differential loss of spinal interneurons in a mouse model of ALS","volume":450,"article_type":"original","date_created":"2020-12-03T11:47:31Z","has_accepted_license":"1","abstract":[{"text":"Amyotrophic lateral sclerosis (ALS) leads to a loss of specific motor neuron populations in the spinal cord and cortex. Emerging evidence suggests that interneurons may also be affected, but a detailed characterization of interneuron loss and its potential impacts on motor neuron loss and disease progression is lacking. To examine this issue, the fate of V1 inhibitory neurons during ALS was assessed in the ventral spinal cord using the SODG93A mouse model. The V1 population makes up ∼30% of all ventral inhibitory neurons, ∼50% of direct inhibitory synaptic contacts onto motor neuron cell bodies, and is thought to play a key role in modulating motor output, in part through recurrent and reciprocal inhibitory circuits. We find that approximately half of V1 inhibitory neurons are lost in SODG93A mice at late disease stages, but that this loss is delayed relative to the loss of motor neurons and V2a excitatory neurons. We further identify V1 subpopulations based on transcription factor expression that are differentially susceptible to degeneration in SODG93A mice. At an early disease stage, we show that V1 synaptic contacts with motor neuron cell bodies increase, suggesting an upregulation of inhibition before V1 neurons are lost in substantial numbers. These data support a model in which progressive changes in V1 synaptic contacts early in disease, and in select V1 subpopulations at later stages, represent a compensatory upregulation and then deleterious breakdown of specific interneuron circuits within the spinal cord.","lang":"eng"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"intvolume":"       450","publication_status":"published","publication_identifier":{"issn":["0306-4522"]},"file_date_updated":"2020-12-03T11:45:26Z","month":"12","department":[{"_id":"LoSw"}],"file":[{"date_updated":"2020-12-03T11:45:26Z","creator":"dernst","date_created":"2020-12-03T11:45:26Z","file_size":4071247,"file_id":"8915","access_level":"open_access","content_type":"application/pdf","success":1,"file_name":"2020_Neuroscience_Salamatina.pdf","checksum":"da7413c819e079720669c82451b49294","relation":"main_file"}],"oa":1,"language":[{"iso":"eng"}],"citation":{"ama":"Salamatina A, Yang JH, Brenner-Morton S, et al. Differential loss of spinal interneurons in a mouse model of ALS. <i>Neuroscience</i>. 2020;450:81-95. doi:<a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">10.1016/j.neuroscience.2020.08.011</a>","ieee":"A. Salamatina <i>et al.</i>, “Differential loss of spinal interneurons in a mouse model of ALS,” <i>Neuroscience</i>, vol. 450. Elsevier, pp. 81–95, 2020.","short":"A. Salamatina, J.H. Yang, S. Brenner-Morton, J.B. Bikoff, L. Fang, C.R. Kintner, T.M. Jessell, L.B. Sweeney, Neuroscience 450 (2020) 81–95.","chicago":"Salamatina, Alina, Jerry H Yang, Susan Brenner-Morton, Jay B  Bikoff, Linjing Fang, Christopher R Kintner, Thomas M Jessell, and Lora B. Sweeney. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” <i>Neuroscience</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">https://doi.org/10.1016/j.neuroscience.2020.08.011</a>.","ista":"Salamatina A, Yang JH, Brenner-Morton S, Bikoff JB, Fang L, Kintner CR, Jessell TM, Sweeney LB. 2020. Differential loss of spinal interneurons in a mouse model of ALS. Neuroscience. 450, 81–95.","apa":"Salamatina, A., Yang, J. H., Brenner-Morton, S., Bikoff, J. B., Fang, L., Kintner, C. R., … Sweeney, L. B. (2020). Differential loss of spinal interneurons in a mouse model of ALS. <i>Neuroscience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">https://doi.org/10.1016/j.neuroscience.2020.08.011</a>","mla":"Salamatina, Alina, et al. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” <i>Neuroscience</i>, vol. 450, Elsevier, 2020, pp. 81–95, doi:<a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">10.1016/j.neuroscience.2020.08.011</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.neuroscience.2020.08.011","article_processing_charge":"Yes (via OA deal)","publisher":"Elsevier","date_updated":"2024-01-31T10:15:34Z","_id":"8914","type":"journal_article","page":"81-95","ddc":["570"],"quality_controlled":"1","isi":1,"year":"2020","external_id":{"isi":["000595588700008"],"pmid":["32858144"]},"status":"public","publication":"Neuroscience","pmid":1,"date_published":"2020-12-01T00:00:00Z","acknowledgement":"This work was made possible by the generous support of Project ALS. Imaging and related analyses were facilitated by The Waitt Advanced Biophotonics Center Core at the Salk Institute, supported by grants from NIH-NCI CCSG (P30 014195) and NINDS Neuroscience Center (NS072031). The authors would like to additionally thank Drs. Jane Dodd, Robert Brownstone, and Laskaro Zagoraiou for helpful comments on the manuscript. This manuscript is dedicated to Tom Jessell, an inspirational scientist, friend and mentor."},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2018-01-04T00:00:00Z","citation":{"mla":"Sweeney, Lora B., et al. “Origin and Segmental Diversity of Spinal Inhibitory Interneurons.” <i>Neuron</i>, vol. 97, no. 2, Elsevier, 2018, p. 341–355.e3, doi:<a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">10.1016/j.neuron.2017.12.029</a>.","apa":"Sweeney, L. B., Bikoff, J. B., Gabitto, M. I., Brenner-Morton, S., Baek, M., Yang, J. H., … Jessell, T. M. (2018). Origin and segmental diversity of spinal inhibitory interneurons. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">https://doi.org/10.1016/j.neuron.2017.12.029</a>","chicago":"Sweeney, Lora B., Jay B. Bikoff, Mariano I. Gabitto, Susan Brenner-Morton, Myungin Baek, Jerry H. Yang, Esteban G. Tabak, Jeremy S. Dasen, Christopher R. Kintner, and Thomas M. Jessell. “Origin and Segmental Diversity of Spinal Inhibitory Interneurons.” <i>Neuron</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">https://doi.org/10.1016/j.neuron.2017.12.029</a>.","ista":"Sweeney LB, Bikoff JB, Gabitto MI, Brenner-Morton S, Baek M, Yang JH, Tabak EG, Dasen JS, Kintner CR, Jessell TM. 2018. Origin and segmental diversity of spinal inhibitory interneurons. Neuron. 97(2), 341–355.e3.","short":"L.B. Sweeney, J.B. Bikoff, M.I. Gabitto, S. Brenner-Morton, M. Baek, J.H. Yang, E.G. Tabak, J.S. Dasen, C.R. Kintner, T.M. Jessell, Neuron 97 (2018) 341–355.e3.","ieee":"L. B. Sweeney <i>et al.</i>, “Origin and segmental diversity of spinal inhibitory interneurons,” <i>Neuron</i>, vol. 97, no. 2. Elsevier, p. 341–355.e3, 2018.","ama":"Sweeney LB, Bikoff JB, Gabitto MI, et al. Origin and segmental diversity of spinal inhibitory interneurons. <i>Neuron</i>. 2018;97(2):341-355.e3. doi:<a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">10.1016/j.neuron.2017.12.029</a>"},"issue":"2","publication":"Neuron","status":"public","extern":"1","language":[{"iso":"eng"}],"month":"01","year":"2018","publication_identifier":{"issn":["0896-6273"]},"publication_status":"published","quality_controlled":"1","intvolume":"        97","abstract":[{"text":"Motor output varies along the rostro-caudal axis of the tetrapod spinal cord. At limb levels, ∼60 motor pools control the alternation of flexor and extensor muscles about each joint, whereas at thoracic levels as few as 10 motor pools supply muscle groups that support posture, inspiration, and expiration. Whether such differences in motor neuron identity and muscle number are associated with segmental distinctions in interneuron diversity has not been resolved. We show that select combinations of nineteen transcription factors that specify lumbar V1 inhibitory interneurons generate subpopulations enriched at limb and thoracic levels. Specification of limb and thoracic V1 interneurons involves the Hox gene Hoxc9 independently of motor neurons. Thus, early Hox patterning of the spinal cord determines the identity of V1 interneurons and motor neurons. These studies reveal a developmental program of V1 interneuron diversity, providing insight into the organization of inhibitory interneurons associated with differential motor output.","lang":"eng"}],"page":"341-355.e3","date_created":"2020-04-30T10:35:13Z","article_type":"original","type":"journal_article","volume":97,"_id":"7698","date_updated":"2024-01-31T10:13:54Z","title":"Origin and segmental diversity of spinal inhibitory interneurons","oa_version":"None","publisher":"Elsevier","article_processing_charge":"No","day":"04","author":[{"full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"},{"first_name":"Jay B.","last_name":"Bikoff","full_name":"Bikoff, Jay B."},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"first_name":"Susan","last_name":"Brenner-Morton","full_name":"Brenner-Morton, Susan"},{"full_name":"Baek, Myungin","last_name":"Baek","first_name":"Myungin"},{"first_name":"Jerry H.","full_name":"Yang, Jerry H.","last_name":"Yang"},{"last_name":"Tabak","full_name":"Tabak, Esteban G.","first_name":"Esteban G."},{"first_name":"Jeremy S.","last_name":"Dasen","full_name":"Dasen, Jeremy S."},{"full_name":"Kintner, Christopher R.","last_name":"Kintner","first_name":"Christopher R."},{"full_name":"Jessell, Thomas M.","last_name":"Jessell","first_name":"Thomas M."}],"doi":"10.1016/j.neuron.2017.12.029"},{"year":"2014","month":"10","citation":{"short":"L.B. Sweeney, D.B. Kelley, Current Opinion in Neurobiology 28 (2014) 34–41.","ieee":"L. B. Sweeney and D. B. Kelley, “Harnessing vocal patterns for social communication,” <i>Current Opinion in Neurobiology</i>, vol. 28, no. 10. Elsevier, pp. 34–41, 2014.","ama":"Sweeney LB, Kelley DB. Harnessing vocal patterns for social communication. <i>Current Opinion in Neurobiology</i>. 2014;28(10):34-41. doi:<a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">10.1016/j.conb.2014.06.006</a>","mla":"Sweeney, Lora B., and Darcy B. Kelley. “Harnessing Vocal Patterns for Social Communication.” <i>Current Opinion in Neurobiology</i>, vol. 28, no. 10, Elsevier, 2014, pp. 34–41, doi:<a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">10.1016/j.conb.2014.06.006</a>.","apa":"Sweeney, L. B., &#38; Kelley, D. B. (2014). Harnessing vocal patterns for social communication. <i>Current Opinion in Neurobiology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">https://doi.org/10.1016/j.conb.2014.06.006</a>","chicago":"Sweeney, Lora B., and Darcy B Kelley. “Harnessing Vocal Patterns for Social Communication.” <i>Current Opinion in Neurobiology</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">https://doi.org/10.1016/j.conb.2014.06.006</a>.","ista":"Sweeney LB, Kelley DB. 2014. Harnessing vocal patterns for social communication. Current Opinion in Neurobiology. 28(10), 34–41."},"issue":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2014-10-01T00:00:00Z","publication":"Current Opinion in Neurobiology","language":[{"iso":"eng"}],"status":"public","extern":"1","_id":"7699","volume":28,"date_updated":"2024-01-31T10:14:08Z","date_created":"2020-04-30T10:35:39Z","type":"journal_article","article_type":"original","day":"01","article_processing_charge":"No","author":[{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"first_name":"Darcy B","full_name":"Kelley, Darcy B","last_name":"Kelley"}],"doi":"10.1016/j.conb.2014.06.006","title":"Harnessing vocal patterns for social communication","oa_version":"None","publisher":"Elsevier","quality_controlled":"1","publication_identifier":{"issn":["0959-4388"]},"publication_status":"published","page":"34-41","intvolume":"        28"},{"year":"2013","month":"05","publication":"Neuron","status":"public","extern":"1","language":[{"iso":"eng"}],"citation":{"ista":"Joo WJ, Sweeney LB, Liang L, Luo L. 2013. Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. Neuron. 78(4), 673–686.","chicago":"Joo, William J., Lora B. Sweeney, Liang Liang, and Liqun Luo. “Linking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting.” <i>Neuron</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">https://doi.org/10.1016/j.neuron.2013.03.022</a>.","mla":"Joo, William J., et al. “Linking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting.” <i>Neuron</i>, vol. 78, no. 4, Elsevier, 2013, pp. 673–86, doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">10.1016/j.neuron.2013.03.022</a>.","apa":"Joo, W. J., Sweeney, L. B., Liang, L., &#38; Luo, L. (2013). Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">https://doi.org/10.1016/j.neuron.2013.03.022</a>","ama":"Joo WJ, Sweeney LB, Liang L, Luo L. Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. <i>Neuron</i>. 2013;78(4):673-686. doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">10.1016/j.neuron.2013.03.022</a>","short":"W.J. Joo, L.B. Sweeney, L. Liang, L. Luo, Neuron 78 (2013) 673–686.","ieee":"W. J. Joo, L. B. Sweeney, L. Liang, and L. Luo, “Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting,” <i>Neuron</i>, vol. 78, no. 4. Elsevier, pp. 673–686, 2013."},"issue":"4","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2013-05-22T00:00:00Z","article_processing_charge":"No","day":"22","author":[{"first_name":"William J.","last_name":"Joo","full_name":"Joo, William J."},{"last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"},{"last_name":"Liang","full_name":"Liang, Liang","first_name":"Liang"},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"}],"doi":"10.1016/j.neuron.2013.03.022","title":"Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting","oa_version":"None","publisher":"Elsevier","volume":78,"_id":"7785","date_updated":"2024-01-31T10:15:25Z","date_created":"2020-04-30T13:19:59Z","type":"journal_article","article_type":"original","page":"673-686","abstract":[{"text":"Neural circuit assembly requires selection of specific cell fates, axonal trajectories, and synaptic targets. By analyzing the function of a secreted semaphorin, Sema-2b, in Drosophila olfactory receptor neuron (ORN) development, we identified multiple molecular and cellular mechanisms that link these events. Notch signaling limits Sema-2b expression to ventromedial ORN classes, within which Sema-2b cell-autonomously sensitizes ORN axons to external semaphorins. Central-brain-derived Sema-2a and Sema-2b attract Sema-2b-expressing axons to the ventromedial trajectory. In addition, Sema-2b/PlexB-mediated axon-axon interactions consolidate this trajectory choice and promote ventromedial axon-bundle formation. Selecting the correct developmental trajectory is ultimately essential for proper target choice. These findings demonstrate that Sema-2b couples ORN axon guidance to postsynaptic target neuron dendrite patterning well before the final target selection phase, and exemplify how a single guidance molecule can drive consecutive stages of neural circuit assembly with the help of sophisticated spatial and temporal regulation.","lang":"eng"}],"intvolume":"        78","publication_status":"published","quality_controlled":"1","publication_identifier":{"issn":["0896-6273"]}},{"article_type":"original","type":"journal_article","date_created":"2020-04-30T10:36:12Z","date_updated":"2024-01-31T10:13:39Z","volume":72,"_id":"7701","oa_version":"None","publisher":"Elsevier","title":"Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting","doi":"10.1016/j.neuron.2011.09.026","author":[{"last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"},{"first_name":"Ya-Hui","full_name":"Chou, Ya-Hui","last_name":"Chou"},{"last_name":"Wu","full_name":"Wu, Zhuhao","first_name":"Zhuhao"},{"first_name":"William","last_name":"Joo","full_name":"Joo, William"},{"last_name":"Komiyama","full_name":"Komiyama, Takaki","first_name":"Takaki"},{"last_name":"Potter","full_name":"Potter, Christopher J.","first_name":"Christopher J."},{"first_name":"Alex L.","full_name":"Kolodkin, Alex L.","last_name":"Kolodkin"},{"last_name":"Garcia","full_name":"Garcia, K. Christopher","first_name":"K. Christopher"},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"}],"article_processing_charge":"No","day":"08","publication_status":"published","quality_controlled":"1","publication_identifier":{"issn":["0896-6273"]},"abstract":[{"text":"During assembly of the Drosophila olfactory circuit, projection neuron (PN) dendrites prepattern the developing antennal lobe before the arrival of axons from their presynaptic partners, the adult olfactory receptor neurons (ORNs). We previously found that levels of transmembrane Semaphorin-1a, which acts as a receptor, instruct PN dendrite targeting along the dorsolateral-ventromedial axis. Here we show that two secreted semaphorins, Sema-2a and Sema-2b, provide spatial cues for PN dendrite targeting. Sema-2a and Sema-2b proteins are distributed in gradients opposing the Sema-1a protein gradient, and Sema-1a binds to Sema-2a-expressing cells. In Sema-2a and Sema-2b double mutants, PN dendrites that normally target dorsolaterally in the antennal lobe mistarget ventromedially, phenocopying cell-autonomous Sema-1a removal from these PNs. Cell ablation, cell-specific knockdown, and rescue experiments indicate that secreted semaphorins from degenerating larval ORN axons direct dendrite targeting. Thus, a degenerating brain structure instructs the wiring of a developing circuit through the repulsive action of secreted semaphorins.","lang":"eng"}],"intvolume":"        72","page":"734-747","month":"12","year":"2011","date_published":"2011-12-08T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"5","citation":{"apa":"Sweeney, L. B., Chou, Y.-H., Wu, Z., Joo, W., Komiyama, T., Potter, C. J., … Luo, L. (2011). Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">https://doi.org/10.1016/j.neuron.2011.09.026</a>","mla":"Sweeney, Lora B., et al. “Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting.” <i>Neuron</i>, vol. 72, no. 5, Elsevier, 2011, pp. 734–47, doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">10.1016/j.neuron.2011.09.026</a>.","chicago":"Sweeney, Lora B., Ya-Hui Chou, Zhuhao Wu, William Joo, Takaki Komiyama, Christopher J. Potter, Alex L. Kolodkin, K. Christopher Garcia, and Liqun Luo. “Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting.” <i>Neuron</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">https://doi.org/10.1016/j.neuron.2011.09.026</a>.","ista":"Sweeney LB, Chou Y-H, Wu Z, Joo W, Komiyama T, Potter CJ, Kolodkin AL, Garcia KC, Luo L. 2011. Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. Neuron. 72(5), 734–747.","ieee":"L. B. Sweeney <i>et al.</i>, “Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting,” <i>Neuron</i>, vol. 72, no. 5. Elsevier, pp. 734–747, 2011.","short":"L.B. Sweeney, Y.-H. Chou, Z. Wu, W. Joo, T. Komiyama, C.J. Potter, A.L. Kolodkin, K.C. Garcia, L. Luo, Neuron 72 (2011) 734–747.","ama":"Sweeney LB, Chou Y-H, Wu Z, et al. Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. <i>Neuron</i>. 2011;72(5):734-747. doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">10.1016/j.neuron.2011.09.026</a>"},"status":"public","extern":"1","publication":"Neuron","language":[{"iso":"eng"}]},{"oa_version":"None","publisher":"Elsevier","title":"A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS","author":[{"last_name":"Wu","full_name":"Wu, Zhuhao","first_name":"Zhuhao"},{"last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"},{"last_name":"Ayoob","full_name":"Ayoob, Joseph C.","first_name":"Joseph C."},{"first_name":"Kayam","last_name":"Chak","full_name":"Chak, Kayam"},{"first_name":"Benjamin J.","last_name":"Andreone","full_name":"Andreone, Benjamin J."},{"full_name":"Ohyama, Tomoko","last_name":"Ohyama","first_name":"Tomoko"},{"last_name":"Kerr","full_name":"Kerr, Rex","first_name":"Rex"},{"first_name":"Liqun","full_name":"Luo, Liqun","last_name":"Luo"},{"last_name":"Zlatic","full_name":"Zlatic, Marta","first_name":"Marta"},{"first_name":"Alex L.","last_name":"Kolodkin","full_name":"Kolodkin, Alex L."}],"doi":"10.1016/j.neuron.2011.02.050","day":"28","article_processing_charge":"No","type":"journal_article","article_type":"original","date_created":"2020-04-30T10:36:30Z","date_updated":"2024-01-31T10:14:29Z","_id":"7702","volume":70,"intvolume":"        70","abstract":[{"lang":"eng","text":"Longitudinal axon fascicles within the Drosophila embryonic CNS provide connections between body segments and are required for coordinated neural signaling along the anterior-posterior axis. We show here that establishment of select CNS longitudinal tracts and formation of precise mechanosensory afferent innervation to the same CNS region are coordinately regulated by the secreted semaphorins Sema-2a and Sema-2b. Both Sema-2a and Sema-2b utilize the same neuronal receptor, plexin B (PlexB), but serve distinct guidance functions. Localized Sema-2b attraction promotes the initial assembly of a subset of CNS longitudinal projections and subsequent targeting of chordotonal sensory afferent axons to these same longitudinal connectives, whereas broader Sema-2a repulsion serves to prevent aberrant innervation. In the absence of Sema-2b or PlexB, chordotonal afferent connectivity within the CNS is severely disrupted, resulting in specific larval behavioral deficits. These results reveal that distinct semaphorin-mediated guidance functions converge at PlexB and are critical for functional neural circuit assembly."}],"page":"281-298","publication_identifier":{"issn":["0896-6273"]},"publication_status":"published","quality_controlled":"1","month":"04","year":"2011","status":"public","publication":"Neuron","extern":"1","language":[{"iso":"eng"}],"date_published":"2011-04-28T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","citation":{"chicago":"Wu, Zhuhao, Lora B. Sweeney, Joseph C. Ayoob, Kayam Chak, Benjamin J. Andreone, Tomoko Ohyama, Rex Kerr, Liqun Luo, Marta Zlatic, and Alex L. Kolodkin. “A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS.” <i>Neuron</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">https://doi.org/10.1016/j.neuron.2011.02.050</a>.","ista":"Wu Z, Sweeney LB, Ayoob JC, Chak K, Andreone BJ, Ohyama T, Kerr R, Luo L, Zlatic M, Kolodkin AL. 2011. A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. Neuron. 70(2), 281–298.","apa":"Wu, Z., Sweeney, L. B., Ayoob, J. C., Chak, K., Andreone, B. J., Ohyama, T., … Kolodkin, A. L. (2011). A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">https://doi.org/10.1016/j.neuron.2011.02.050</a>","mla":"Wu, Zhuhao, et al. “A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS.” <i>Neuron</i>, vol. 70, no. 2, Elsevier, 2011, pp. 281–98, doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">10.1016/j.neuron.2011.02.050</a>.","ama":"Wu Z, Sweeney LB, Ayoob JC, et al. A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. <i>Neuron</i>. 2011;70(2):281-298. doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">10.1016/j.neuron.2011.02.050</a>","ieee":"Z. Wu <i>et al.</i>, “A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS,” <i>Neuron</i>, vol. 70, no. 2. Elsevier, pp. 281–298, 2011.","short":"Z. Wu, L.B. Sweeney, J.C. Ayoob, K. Chak, B.J. Andreone, T. Ohyama, R. Kerr, L. Luo, M. Zlatic, A.L. Kolodkin, Neuron 70 (2011) 281–298."}},{"title":"‘Fore brain: A hint of the ancestral cortex","publisher":"Elsevier","oa_version":"None","day":"03","article_processing_charge":"No","doi":"10.1016/j.cell.2010.08.024","author":[{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger"},{"last_name":"Luo","full_name":"Luo, Liqun","first_name":"Liqun"}],"date_created":"2020-04-30T10:36:52Z","article_type":"original","type":"journal_article","_id":"7703","volume":142,"date_updated":"2024-01-31T10:14:59Z","abstract":[{"text":"By combining gene expression profiling with image registration, Tomer et al. (2010) find that the mushroom body of the segmented worm Platynereis dumerilii shares many features with the mammalian cerebral cortex. The authors propose that the mushroom body and cortex evolved from the same structure in the common ancestor of vertebrates and invertebrates.","lang":"eng"}],"intvolume":"       142","page":"679-681","publication_status":"published","quality_controlled":"1","publication_identifier":{"issn":["0092-8674"]},"month":"09","year":"2010","status":"public","language":[{"iso":"eng"}],"extern":"1","publication":"Cell","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2010-09-03T00:00:00Z","citation":{"ieee":"L. B. Sweeney and L. Luo, “‘Fore brain: A hint of the ancestral cortex,” <i>Cell</i>, vol. 142, no. 5. Elsevier, pp. 679–681, 2010.","short":"L.B. Sweeney, L. Luo, Cell 142 (2010) 679–681.","ama":"Sweeney LB, Luo L. ‘Fore brain: A hint of the ancestral cortex. <i>Cell</i>. 2010;142(5):679-681. doi:<a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">10.1016/j.cell.2010.08.024</a>","apa":"Sweeney, L. B., &#38; Luo, L. (2010). ‘Fore brain: A hint of the ancestral cortex. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">https://doi.org/10.1016/j.cell.2010.08.024</a>","mla":"Sweeney, Lora B., and Liqun Luo. “‘Fore Brain: A Hint of the Ancestral Cortex.” <i>Cell</i>, vol. 142, no. 5, Elsevier, 2010, pp. 679–81, doi:<a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">10.1016/j.cell.2010.08.024</a>.","ista":"Sweeney LB, Luo L. 2010. ‘Fore brain: A hint of the ancestral cortex. Cell. 142(5), 679–681.","chicago":"Sweeney, Lora B., and Liqun Luo. “‘Fore Brain: A Hint of the Ancestral Cortex.” <i>Cell</i>. Elsevier, 2010. <a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">https://doi.org/10.1016/j.cell.2010.08.024</a>."},"issue":"5"},{"month":"01","year":"2007","date_published":"2007-01-26T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","citation":{"short":"T. Komiyama, L.B. Sweeney, O. Schuldiner, K.C. Garcia, L. Luo, Cell 128 (2007) 399–410.","ieee":"T. Komiyama, L. B. Sweeney, O. Schuldiner, K. C. Garcia, and L. Luo, “Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons,” <i>Cell</i>, vol. 128, no. 2. Elsevier, pp. 399–410, 2007.","ama":"Komiyama T, Sweeney LB, Schuldiner O, Garcia KC, Luo L. Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. <i>Cell</i>. 2007;128(2):399-410. doi:<a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">10.1016/j.cell.2006.12.028</a>","mla":"Komiyama, Takaki, et al. “Graded Expression of Semaphorin-1a Cell-Autonomously Directs Dendritic Targeting of Olfactory Projection Neurons.” <i>Cell</i>, vol. 128, no. 2, Elsevier, 2007, pp. 399–410, doi:<a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">10.1016/j.cell.2006.12.028</a>.","apa":"Komiyama, T., Sweeney, L. B., Schuldiner, O., Garcia, K. C., &#38; Luo, L. (2007). Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">https://doi.org/10.1016/j.cell.2006.12.028</a>","chicago":"Komiyama, Takaki, Lora B. Sweeney, Oren Schuldiner, K. Christopher Garcia, and Liqun Luo. “Graded Expression of Semaphorin-1a Cell-Autonomously Directs Dendritic Targeting of Olfactory Projection Neurons.” <i>Cell</i>. Elsevier, 2007. <a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">https://doi.org/10.1016/j.cell.2006.12.028</a>.","ista":"Komiyama T, Sweeney LB, Schuldiner O, Garcia KC, Luo L. 2007. Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. Cell. 128(2), 399–410."},"language":[{"iso":"eng"}],"status":"public","publication":"Cell","extern":"1","type":"journal_article","article_type":"original","date_created":"2020-04-30T10:37:08Z","date_updated":"2024-01-31T10:14:48Z","_id":"7704","volume":128,"title":"Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons","publisher":"Elsevier","oa_version":"None","author":[{"last_name":"Komiyama","full_name":"Komiyama, Takaki","first_name":"Takaki"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"first_name":"Oren","last_name":"Schuldiner","full_name":"Schuldiner, Oren"},{"first_name":"K. Christopher","full_name":"Garcia, K. Christopher","last_name":"Garcia"},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"}],"doi":"10.1016/j.cell.2006.12.028","day":"26","article_processing_charge":"No","quality_controlled":"1","publication_status":"published","publication_identifier":{"issn":["0092-8674"]},"abstract":[{"lang":"eng","text":"Gradients of axon guidance molecules instruct the formation of continuous neural maps, such as the retinotopic map in the vertebrate visual system. Here we show that molecular gradients can also instruct the formation of a discrete neural map. In the fly olfactory system, axons of 50 classes of olfactory receptor neurons (ORNs) and dendrites of 50 classes of projection neurons (PNs) form one-to-one connections at discrete units called glomeruli. We provide expression, loss- and gain-of-function data to demonstrate that the levels of transmembrane Semaphorin-1a (Sema-1a), acting cell-autonomously as a receptor or part of a receptor complex, direct the dendritic targeting of PNs along the dorsolateral to ventromedial axis of the antennal lobe. Sema-1a also regulates PN axon targeting in higher olfactory centers. Thus, graded expression of Sema-1a contributes to connection specificity from ORNs to PNs and then to higher brain centers, ensuring proper representation of olfactory information in the brain."}],"intvolume":"       128","page":"399-410"},{"month":"01","year":"2007","date_published":"2007-01-18T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","citation":{"ista":"Sweeney LB, Couto A, Chou Y-H, Berdnik D, Dickson BJ, Luo L, Komiyama T. 2007. Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. Neuron. 53(2), 185–200.","chicago":"Sweeney, Lora B., Africa Couto, Ya-Hui Chou, Daniela Berdnik, Barry J. Dickson, Liqun Luo, and Takaki Komiyama. “Temporal Target Restriction of Olfactory Receptor Neurons by Semaphorin-1a/PlexinA-Mediated Axon-Axon Interactions.” <i>Neuron</i>. Elsevier, 2007. <a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">https://doi.org/10.1016/j.neuron.2006.12.022</a>.","apa":"Sweeney, L. B., Couto, A., Chou, Y.-H., Berdnik, D., Dickson, B. J., Luo, L., &#38; Komiyama, T. (2007). Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">https://doi.org/10.1016/j.neuron.2006.12.022</a>","mla":"Sweeney, Lora B., et al. “Temporal Target Restriction of Olfactory Receptor Neurons by Semaphorin-1a/PlexinA-Mediated Axon-Axon Interactions.” <i>Neuron</i>, vol. 53, no. 2, Elsevier, 2007, pp. 185–200, doi:<a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">10.1016/j.neuron.2006.12.022</a>.","ama":"Sweeney LB, Couto A, Chou Y-H, et al. Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. <i>Neuron</i>. 2007;53(2):185-200. doi:<a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">10.1016/j.neuron.2006.12.022</a>","ieee":"L. B. Sweeney <i>et al.</i>, “Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions,” <i>Neuron</i>, vol. 53, no. 2. Elsevier, pp. 185–200, 2007.","short":"L.B. Sweeney, A. Couto, Y.-H. Chou, D. Berdnik, B.J. Dickson, L. Luo, T. Komiyama, Neuron 53 (2007) 185–200."},"extern":"1","status":"public","publication":"Neuron","language":[{"iso":"eng"}],"article_type":"original","type":"journal_article","date_created":"2020-04-30T10:37:24Z","date_updated":"2024-01-31T10:14:39Z","volume":53,"_id":"7705","oa_version":"None","publisher":"Elsevier","title":"Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions","author":[{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney"},{"last_name":"Couto","full_name":"Couto, Africa","first_name":"Africa"},{"first_name":"Ya-Hui","full_name":"Chou, Ya-Hui","last_name":"Chou"},{"full_name":"Berdnik, Daniela","last_name":"Berdnik","first_name":"Daniela"},{"first_name":"Barry J.","full_name":"Dickson, Barry J.","last_name":"Dickson"},{"full_name":"Luo, Liqun","last_name":"Luo","first_name":"Liqun"},{"first_name":"Takaki","last_name":"Komiyama","full_name":"Komiyama, Takaki"}],"doi":"10.1016/j.neuron.2006.12.022","day":"18","article_processing_charge":"No","publication_status":"published","quality_controlled":"1","publication_identifier":{"issn":["0896-6273"]},"intvolume":"        53","abstract":[{"text":"Axon-axon interactions have been implicated in neural circuit assembly, but the underlying mechanisms are poorly understood. Here, we show that in the Drosophila antennal lobe, early-arriving axons of olfactory receptor neurons (ORNs) from the antenna are required for the proper targeting of late-arriving ORN axons from the maxillary palp (MP). Semaphorin-1a is required for targeting of all MP but only half of the antennal ORN classes examined. Sema-1a acts nonautonomously to control ORN axon-axon interactions, in contrast to its cell-autonomous function in olfactory projection neurons. Phenotypic and genetic interaction analyses implicate PlexinA as the Sema-1a receptor in ORN targeting. Sema-1a on antennal ORN axons is required for correct targeting of MP axons within the antennal lobe, while interactions amongst MP axons facilitate their entry into the antennal lobe. We propose that Sema-1a/PlexinA-mediated repulsion provides a mechanism by which early-arriving ORN axons constrain the target choices of late-arriving axons.","lang":"eng"}],"page":"185-200"},{"publication_identifier":{"issn":["0036-8075","1095-9203"]},"publication_status":"published","quality_controlled":"1","page":"2011-2015","abstract":[{"lang":"eng","text":"The Sir2 deacetylase modulates organismal life-span in various species. However, the molecular mechanisms by which Sir2 increases longevity are largely unknown. We show that in mammalian cells, the Sir2 homolog SIRT1 appears to control the cellular response to stress by regulating the FOXO family of Forkhead transcription factors, a family of proteins that function as sensors of the insulin signaling pathway and as regulators of organismal longevity. SIRT1 and the FOXO transcription factor FOXO3 formed a complex in cells in response to oxidative stress, and SIRT1 deacetylated FOXO3 in vitro and within cells. SIRT1 had a dual effect on FOXO3 function: SIRT1 increased FOXO3's ability to induce cell cycle arrest and resistance to oxidative stress but inhibited FOXO3's ability to induce cell death. Thus, one way in which members of the Sir2 family of proteins may increase organismal longevity is by tipping FOXO-dependent responses away from apoptosis and toward stress resistance."}],"intvolume":"       303","date_updated":"2024-01-31T10:14:17Z","volume":303,"_id":"7706","type":"journal_article","article_type":"original","date_created":"2020-04-30T10:37:41Z","doi":"10.1126/science.1094637","author":[{"first_name":"Anne","last_name":"Brunet","full_name":"Brunet, Anne"},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"first_name":"J Fitzhugh ","full_name":"Sturgill, J Fitzhugh ","last_name":"Sturgill"},{"full_name":"Chua, Katrin","last_name":"Chua","first_name":"Katrin"},{"first_name":"Paul","last_name":"Greer","full_name":"Greer, Paul"},{"last_name":"Lin","full_name":"Lin, Yingxi","first_name":"Yingxi"},{"full_name":"Tran, Hien","last_name":"Tran","first_name":"Hien"},{"first_name":"Sarah","last_name":"Ross","full_name":"Ross, Sarah"},{"full_name":"Mostoslavsky, Raul","last_name":"Mostoslavsky","first_name":"Raul"},{"first_name":"Haim","last_name":"Cohen","full_name":"Cohen, Haim"},{"last_name":"Hu","full_name":"Hu, Linda","first_name":"Linda"},{"first_name":"Hwei-Ling","last_name":"Chen","full_name":"Chen, Hwei-Ling"},{"full_name":"Jedrychowski, Mark","last_name":"Jedrychowski","first_name":"Mark"},{"first_name":"Steven","last_name":"Gygi","full_name":"Gygi, Steven"},{"last_name":"Sinclair","full_name":"Sinclair, David","first_name":"David"},{"full_name":"Alt, Frederick","last_name":"Alt","first_name":"Frederick"},{"full_name":"Greenberg, Michael","last_name":"Greenberg","first_name":"Michael"}],"article_processing_charge":"No","day":"26","oa_version":"None","publisher":"American Association for the Advancement of Science","title":"Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase","issue":"5666","citation":{"short":"A. Brunet, L.B. Sweeney, J.F. Sturgill, K. Chua, P. Greer, Y. Lin, H. Tran, S. Ross, R. Mostoslavsky, H. Cohen, L. Hu, H.-L. Chen, M. Jedrychowski, S. Gygi, D. Sinclair, F. Alt, M. Greenberg, Science 303 (2004) 2011–2015.","ieee":"A. Brunet <i>et al.</i>, “Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase,” <i>Science</i>, vol. 303, no. 5666. American Association for the Advancement of Science, pp. 2011–2015, 2004.","ama":"Brunet A, Sweeney LB, Sturgill JF, et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. <i>Science</i>. 2004;303(5666):2011-2015. doi:<a href=\"https://doi.org/10.1126/science.1094637\">10.1126/science.1094637</a>","mla":"Brunet, Anne, et al. “Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase.” <i>Science</i>, vol. 303, no. 5666, American Association for the Advancement of Science, 2004, pp. 2011–15, doi:<a href=\"https://doi.org/10.1126/science.1094637\">10.1126/science.1094637</a>.","apa":"Brunet, A., Sweeney, L. B., Sturgill, J. F., Chua, K., Greer, P., Lin, Y., … Greenberg, M. (2004). Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1094637\">https://doi.org/10.1126/science.1094637</a>","chicago":"Brunet, Anne, Lora B. Sweeney, J Fitzhugh  Sturgill, Katrin Chua, Paul Greer, Yingxi Lin, Hien Tran, et al. “Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase.” <i>Science</i>. American Association for the Advancement of Science, 2004. <a href=\"https://doi.org/10.1126/science.1094637\">https://doi.org/10.1126/science.1094637</a>.","ista":"Brunet A, Sweeney LB, Sturgill JF, Chua K, Greer P, Lin Y, Tran H, Ross S, Mostoslavsky R, Cohen H, Hu L, Chen H-L, Jedrychowski M, Gygi S, Sinclair D, Alt F, Greenberg M. 2004. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 303(5666), 2011–2015."},"date_published":"2004-03-26T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Science","extern":"1","status":"public","language":[{"iso":"eng"}],"year":"2004","month":"03"}]