[{"publication":"Cell Reports","acknowledgement":"We are grateful to members of the C.-P.H. and H.J. labs for discussions, R. Hauschild and the different Scientific Service Units at IST Austria for technical help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce, and L. Solnica-Krezel for sending plasmids. This work was supported by grants from the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H., and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon Areces fellowship.","publist_id":"6275","project":[{"name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17","call_identifier":"FWF"},{"call_identifier":"FWF","grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation"},{"name":"Microbial Ion Channels for Synthetic Neurobiology","_id":"25548C20-B435-11E9-9278-68D0E5697425","grant_number":"303564","call_identifier":"FP7"}],"publisher":"Cell Press","has_accepted_license":"1","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"title":"Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation","status":"public","file_date_updated":"2018-12-12T10:11:04Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","day":"19","oa":1,"citation":{"short":"K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht, D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016) 866–877.","ieee":"K. Sako <i>et al.</i>, “Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation,” <i>Cell Reports</i>, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.","chicago":"Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">https://doi.org/10.1016/j.celrep.2016.06.036</a>.","apa":"Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht, V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">https://doi.org/10.1016/j.celrep.2016.06.036</a>","mla":"Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:<a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">10.1016/j.celrep.2016.06.036</a>.","ama":"Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. 2016;16(3):866-877. doi:<a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">10.1016/j.celrep.2016.06.036</a>","ista":"Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 16(3), 866–877."},"quality_controlled":"1","file":[{"file_name":"IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf","content_type":"application/pdf","relation":"main_file","creator":"system","file_id":"4857","file_size":3921947,"access_level":"open_access","date_created":"2018-12-12T10:11:04Z","date_updated":"2018-12-12T10:11:04Z"}],"page":"866 - 877","volume":16,"oa_version":"Published Version","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"month":"07","language":[{"iso":"eng"}],"doi":"10.1016/j.celrep.2016.06.036","year":"2016","ddc":["570","576"],"acknowledged_ssus":[{"_id":"SSU"}],"type":"journal_article","date_updated":"2024-03-25T23:30:13Z","publication_status":"published","intvolume":"        16","pubrep_id":"754","related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"},{"id":"50","status":"public","relation":"dissertation_contains"}]},"abstract":[{"text":"During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.","lang":"eng"}],"author":[{"id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","full_name":"Sako, Keisuke","orcid":"0000-0002-6453-8075","last_name":"Sako","first_name":"Keisuke"},{"full_name":"Pradhan, Saurabh","first_name":"Saurabh","last_name":"Pradhan"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","first_name":"Vanessa","orcid":"0000-0003-2676-3367","last_name":"Barone"},{"id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","full_name":"Inglés Prieto, Álvaro","first_name":"Álvaro","last_name":"Inglés Prieto","orcid":"0000-0002-5409-8571"},{"full_name":"Mueller, Patrick","first_name":"Patrick","last_name":"Mueller"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","first_name":"Verena"},{"orcid":"0000-0001-5199-9940","last_name":"Capek","first_name":"Daniel","full_name":"Capek, Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Galande","first_name":"Sanjeev","full_name":"Galande, Sanjeev"},{"id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","first_name":"Harald L","last_name":"Janovjak","orcid":"0000-0002-8023-9315","full_name":"Janovjak, Harald L"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"ec_funded":1,"issue":"3","date_created":"2018-12-11T11:50:08Z","_id":"1100","date_published":"2016-07-19T00:00:00Z","scopus_import":1,"license":"https://creativecommons.org/licenses/by/4.0/"},{"date_published":"2016-01-15T00:00:00Z","scopus_import":1,"oa_version":"None","volume":116,"_id":"1239","date_created":"2018-12-11T11:50:53Z","issue":"2","author":[{"full_name":"Callan Jones, Andrew","last_name":"Callan Jones","first_name":"Andrew"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena"},{"full_name":"Wieser, Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"}],"abstract":[{"lang":"eng","text":"Nonadherent polarized cells have been observed to have a pearlike, elongated shape. Using a minimal model that describes the cell cortex as a thin layer of contractile active gel, we show that the anisotropy of active stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes can be determined from the flow pattern only; they prove to be independent of the mechanism at the origin of the cortical flow, and are only weakly sensitive to the cytoplasmic rheology. In the case of actin flows resulting from a contractile instability, we propose a phase diagram of three-dimensional cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate shapes, all of which have been observed in experiment."}],"quality_controlled":"1","citation":{"short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 116 (2016).","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">https://doi.org/10.1103/PhysRevLett.116.028102</a>.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Cortical flow-driven shapes of nonadherent cells,” <i>Physical Review Letters</i>, vol. 116, no. 2. American Physical Society, 2016.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Cortical flow-driven shapes of nonadherent cells. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">https://doi.org/10.1103/PhysRevLett.116.028102</a>","mla":"Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.” <i>Physical Review Letters</i>, vol. 116, no. 2, 028102, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">10.1103/PhysRevLett.116.028102</a>.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical flow-driven shapes of nonadherent cells. <i>Physical Review Letters</i>. 2016;116(2). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">10.1103/PhysRevLett.116.028102</a>","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2), 028102."},"article_number":"028102","day":"15","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_status":"published","intvolume":"       116","department":[{"_id":"CaHe"}],"title":"Cortical flow-driven shapes of nonadherent cells","status":"public","type":"journal_article","date_updated":"2021-01-12T06:49:19Z","publisher":"American Physical Society","project":[{"name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"T 560-B17"}],"acknowledgement":"V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant No. T560-B17).","doi":"10.1103/PhysRevLett.116.028102","publist_id":"6095","year":"2016","language":[{"iso":"eng"}],"publication":"Physical Review Letters","month":"01"},{"publisher":"American Physical Society","type":"journal_article","date_updated":"2021-01-12T06:49:33Z","publication_status":"published","department":[{"_id":"CaHe"}],"intvolume":"       117","title":"Callan-Jones et al. Reply","status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","month":"09","publication":"Physical Review Letters","language":[{"iso":"eng"}],"doi":"10.1103/PhysRevLett.117.139802","publist_id":"6041","year":"2016","date_created":"2018-12-11T11:51:05Z","volume":117,"oa_version":"None","_id":"1275","date_published":"2016-09-22T00:00:00Z","scopus_import":1,"article_number":"139802","day":"22","citation":{"ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.","mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. <i>Physical Review Letters</i>. 2016;117(13). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Callan-Jones et al. Reply. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” <i>Physical Review Letters</i>, vol. 117, no. 13. American Physical Society, 2016.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016)."},"quality_controlled":"1","author":[{"full_name":"Callan Jones, Andrew","first_name":"Andrew","last_name":"Callan Jones"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633"},{"first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"}],"issue":"13"},{"tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"volume":160,"page":"673 - 685","oa_version":"Published Version","file":[{"file_name":"IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf","content_type":"application/pdf","relation":"main_file","creator":"system","file_id":"5003","file_size":4362653,"checksum":"228d3edf40627d897b3875088a0ac51f","access_level":"open_access","date_created":"2018-12-12T10:13:21Z","date_updated":"2020-07-14T12:45:01Z"}],"quality_controlled":"1","citation":{"chicago":"Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">https://doi.org/10.1016/j.cell.2015.01.008</a>.","ieee":"V. Ruprecht <i>et al.</i>, “Cortical contractility triggers a stochastic switch to fast amoeboid cell motility,” <i>Cell</i>, vol. 160, no. 4. Cell Press, pp. 673–685, 2015.","short":"V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V. Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160 (2015) 673–685.","ista":"Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V, Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.","apa":"Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K., … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">https://doi.org/10.1016/j.cell.2015.01.008</a>","mla":"Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” <i>Cell</i>, vol. 160, no. 4, Cell Press, 2015, pp. 673–85, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">10.1016/j.cell.2015.01.008</a>.","ama":"Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. <i>Cell</i>. 2015;160(4):673-685. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">10.1016/j.cell.2015.01.008</a>"},"oa":1,"day":"12","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2020-07-14T12:45:01Z","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"title":"Cortical contractility triggers a stochastic switch to fast amoeboid cell motility","status":"public","has_accepted_license":"1","publisher":"Cell Press","project":[{"_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17","call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation"},{"name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","call_identifier":"FWF","grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425"}],"publist_id":"5634","acknowledgement":"We would like to thank R. Hausschild and E. Papusheva for technical assistance and the service facilities at the IST Austria for continuous support. The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel and E. Paluch for exchanging unpublished data. ","publication":"Cell","scopus_import":1,"date_published":"2015-02-12T00:00:00Z","_id":"1537","date_created":"2018-12-11T11:52:35Z","issue":"4","author":[{"full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217","last_name":"Wieser","first_name":"Stefan","full_name":"Wieser, Stefan"},{"full_name":"Callan Jones, Andrew","first_name":"Andrew","last_name":"Callan Jones"},{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael","first_name":"Michael","last_name":"Smutny","orcid":"0000-0002-5920-9090"},{"last_name":"Morita","first_name":"Hitoshi","full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87"},{"id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","first_name":"Keisuke","orcid":"0000-0002-6453-8075","last_name":"Sako","full_name":"Sako, Keisuke"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","orcid":"0000-0003-2676-3367","last_name":"Barone","full_name":"Barone, Vanessa"},{"full_name":"Ritsch Marte, Monika","first_name":"Monika","last_name":"Ritsch Marte"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.","lang":"eng"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"961"}]},"pubrep_id":"484","intvolume":"       160","publication_status":"published","date_updated":"2023-09-07T12:05:08Z","type":"journal_article","acknowledged_ssus":[{"_id":"SSU"}],"ddc":["570"],"year":"2015","doi":"10.1016/j.cell.2015.01.008","language":[{"iso":"eng"}],"month":"02"},{"publisher":"Cell Press","date_updated":"2021-01-12T06:51:33Z","type":"journal_article","title":"Actin flows mediate a universal coupling between cell speed and cell persistence","status":"public","intvolume":"       161","department":[{"_id":"MiSi"},{"_id":"CaHe"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Cell","month":"04","language":[{"iso":"eng"}],"publist_id":"5618","year":"2015","doi":"10.1016/j.cell.2015.01.056","project":[{"name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","grant_number":"T 560-B17","call_identifier":"FWF","_id":"2529486C-B435-11E9-9278-68D0E5697425"},{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)"},{"name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425","grant_number":"RGP0058/2011"}],"date_created":"2018-12-11T11:52:41Z","_id":"1553","oa_version":"None","volume":161,"page":"374 - 386","scopus_import":1,"date_published":"2015-04-09T00:00:00Z","day":"09","citation":{"short":"P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M. Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger, M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez, Cell 161 (2015) 374–386.","ieee":"P. Maiuri <i>et al.</i>, “Actin flows mediate a universal coupling between cell speed and cell persistence,” <i>Cell</i>, vol. 161, no. 2. Cell Press, pp. 374–386, 2015.","chicago":"Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>.","mla":"Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>, vol. 161, no. 2, Cell Press, 2015, pp. 374–86, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>.","ama":"Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. 2015;161(2):374-386. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>","apa":"Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N., … Voituriez, R. (2015). Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>","ista":"Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M, De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 161(2), 374–386."},"abstract":[{"text":"Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns.","lang":"eng"}],"quality_controlled":"1","author":[{"full_name":"Maiuri, Paolo","last_name":"Maiuri","first_name":"Paolo"},{"first_name":"Jean","last_name":"Rupprecht","full_name":"Rupprecht, Jean"},{"orcid":"0000-0002-2670-2217","last_name":"Wieser","first_name":"Stefan","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena"},{"first_name":"Olivier","last_name":"Bénichou","full_name":"Bénichou, Olivier"},{"last_name":"Carpi","first_name":"Nicolas","full_name":"Carpi, Nicolas"},{"full_name":"Coppey, Mathieu","first_name":"Mathieu","last_name":"Coppey"},{"first_name":"Simon","last_name":"De Beco","full_name":"De Beco, Simon"},{"first_name":"Nir","last_name":"Gov","full_name":"Gov, Nir"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Lage Crespo, Carolina","first_name":"Carolina","last_name":"Lage Crespo"},{"full_name":"Lautenschlaeger, Franziska","last_name":"Lautenschlaeger","first_name":"Franziska"},{"full_name":"Le Berre, Maël","last_name":"Le Berre","first_name":"Maël"},{"first_name":"Ana","last_name":"Lennon Duménil","full_name":"Lennon Duménil, Ana"},{"full_name":"Raab, Matthew","first_name":"Matthew","last_name":"Raab"},{"full_name":"Thiam, Hawa","last_name":"Thiam","first_name":"Hawa"},{"last_name":"Piel","first_name":"Matthieu","full_name":"Piel, Matthieu"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"}],"ec_funded":1,"issue":"2"},{"title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","has_accepted_license":"1","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2020-07-14T12:45:21Z","publisher":"IOP Publishing","publist_id":"5169","acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","publication":"Nanotechnology","article_processing_charge":"No","file":[{"access_level":"open_access","checksum":"df4e03d225a19179e7790f6d87a12332","date_updated":"2020-07-14T12:45:21Z","date_created":"2020-05-15T09:21:19Z","creator":"dernst","file_id":"7856","content_type":"application/pdf","relation":"main_file","file_name":"2014_Nanotechnology_Lamprecht.pdf","file_size":3804152}],"article_type":"original","volume":25,"oa_version":"Submitted Version","day":"28","article_number":"125704","citation":{"chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>.","ieee":"C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>, vol. 25, no. 12. IOP Publishing, 2014.","short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.","apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. 2014;25(12). doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>."},"oa":1,"intvolume":"        25","publication_status":"published","date_updated":"2021-01-12T06:54:07Z","type":"journal_article","year":"2014","doi":"10.1088/0957-4484/25/12/125704","ddc":["570"],"month":"03","language":[{"iso":"eng"}],"scopus_import":1,"date_published":"2014-03-28T00:00:00Z","date_created":"2018-12-11T11:54:45Z","_id":"1925","abstract":[{"lang":"eng","text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity."}],"author":[{"full_name":"Lamprecht, Constanze","first_name":"Constanze","last_name":"Lamprecht"},{"full_name":"Plochberger, Birgit","first_name":"Birgit","last_name":"Plochberger"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217"},{"first_name":"Christian","last_name":"Rankl","full_name":"Rankl, Christian"},{"full_name":"Heister, Elena","last_name":"Heister","first_name":"Elena"},{"full_name":"Unterauer, Barbara","last_name":"Unterauer","first_name":"Barbara"},{"last_name":"Brameshuber","first_name":"Mario","full_name":"Brameshuber, Mario"},{"full_name":"Danzberger, Jürgen","last_name":"Danzberger","first_name":"Jürgen"},{"first_name":"Petar","last_name":"Lukanov","full_name":"Lukanov, Petar"},{"full_name":"Flahaut, Emmanuel","last_name":"Flahaut","first_name":"Emmanuel"},{"full_name":"Schütz, Gerhard","first_name":"Gerhard","last_name":"Schütz"},{"full_name":"Hinterdorfer, Peter","first_name":"Peter","last_name":"Hinterdorfer"},{"last_name":"Ebner","first_name":"Andreas","full_name":"Ebner, Andreas"}],"issue":"12"},{"publisher":"Springer","title":"UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo","department":[{"_id":"CaHe"}],"status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication":"Tissue Morphogenesis","article_processing_charge":"No","external_id":{"pmid":["25245697"]},"page":"219-235","volume":1189,"oa_version":"None","series_title":"Methods in Molecular Biology","day":"22","citation":{"short":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:, C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.","ieee":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg, “UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo,” in <i>Tissue Morphogenesis</i>, vol. 1189, C. Nelson, Ed. New York, NY: Springer, 2014, pp. 219–235.","chicago":"Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York, NY: Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>.","mla":"Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>.","ama":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Nelson C, ed. <i>Tissue Morphogenesis</i>. Vol 1189. Methods in Molecular Biology. New York, NY: Springer; 2014:219-235. doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>","apa":"Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., &#38; Heisenberg, C.-P. J. (2014). UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), <i>Tissue Morphogenesis</i> (Vol. 1189, pp. 219–235). New York, NY: Springer. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>","ista":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235."},"quality_controlled":"1","publication_identifier":{"issn":["1064-3745"],"isbn":["9781493911639","9781493911646"],"eissn":["1940-6029"]},"date_updated":"2023-09-05T14:12:00Z","type":"book_chapter","intvolume":"      1189","publication_status":"published","place":"New York, NY","month":"08","language":[{"iso":"eng"}],"year":"2014","doi":"10.1007/978-1-4939-1164-6_15","date_created":"2019-03-26T08:55:59Z","editor":[{"full_name":"Nelson, Celeste","last_name":"Nelson","first_name":"Celeste"}],"_id":"6178","date_published":"2014-08-22T00:00:00Z","pmid":1,"author":[{"full_name":"Smutny, Michael","orcid":"0000-0002-5920-9090","last_name":"Smutny","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Behrndt","full_name":"Behrndt, Martin"},{"last_name":"Campinho","orcid":"0000-0002-8526-5416","first_name":"Pedro","full_name":"Campinho, Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"abstract":[{"text":"Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.","lang":"eng"}]},{"date_published":"2011-06-08T00:00:00Z","_id":"3285","oa_version":"None","volume":100,"page":"2839 - 2845","date_created":"2018-12-11T12:02:27Z","issue":"11","author":[{"full_name":"Ruprecht, Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217"},{"last_name":"Marguet","first_name":"Didier","full_name":"Marguet, Didier"},{"last_name":"Schuetz","first_name":"Gerhard","full_name":"Schuetz, Gerhard"}],"abstract":[{"lang":"eng","text":"Resolving the dynamical interplay of proteins and lipids in the live-cell plasma membrane represents a central goal in current cell biology. Superresolution concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic length scale. Similar concepts for detecting dynamical transitions (superresolution chronoscopy) are still lacking. Here, we show that recently introduced spot-variation fluorescence correlation spectroscopy allows for sensing transient confinement times of membrane constituents at dramatically improved resolution. Using standard diffraction-limited optics, spot-variation fluorescence correlation spectroscopy captures signatures of single retardation events far below the transit time of the tracer through the focal spot. We provide an analytical description of special cases of transient binding of a tracer to pointlike traps, or association of a tracer with nanodomains. The influence of trap mobility and the underlying binding kinetics are quantified. Experimental approaches are suggested that allow for gaining quantitative mechanistic insights into the interaction processes of membrane constituents."}],"citation":{"ista":"Ruprecht V, Wieser S, Marguet D, Schuetz G. 2011. Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes. Biophysical Journal. 100(11), 2839–2845.","apa":"Ruprecht, V., Wieser, S., Marguet, D., &#38; Schuetz, G. (2011). Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes. <i>Biophysical Journal</i>. Biophysical Society. <a href=\"https://doi.org/10.1016/j.bpj.2011.04.035\">https://doi.org/10.1016/j.bpj.2011.04.035</a>","ama":"Ruprecht V, Wieser S, Marguet D, Schuetz G. Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes. <i>Biophysical Journal</i>. 2011;100(11):2839-2845. doi:<a href=\"https://doi.org/10.1016/j.bpj.2011.04.035\">10.1016/j.bpj.2011.04.035</a>","mla":"Ruprecht, Verena, et al. “Spot Variation Fluorescence Correlation Spectroscopy Allows for Superresolution Chronoscopy of Confinement Times in Membranes.” <i>Biophysical Journal</i>, vol. 100, no. 11, Biophysical Society, 2011, pp. 2839–45, doi:<a href=\"https://doi.org/10.1016/j.bpj.2011.04.035\">10.1016/j.bpj.2011.04.035</a>.","chicago":"Ruprecht, Verena, Stefan Wieser, Didier Marguet, and Gerhard Schuetz. “Spot Variation Fluorescence Correlation Spectroscopy Allows for Superresolution Chronoscopy of Confinement Times in Membranes.” <i>Biophysical Journal</i>. Biophysical Society, 2011. <a href=\"https://doi.org/10.1016/j.bpj.2011.04.035\">https://doi.org/10.1016/j.bpj.2011.04.035</a>.","ieee":"V. Ruprecht, S. Wieser, D. Marguet, and G. Schuetz, “Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes,” <i>Biophysical Journal</i>, vol. 100, no. 11. Biophysical Society, pp. 2839–2845, 2011.","short":"V. Ruprecht, S. Wieser, D. Marguet, G. Schuetz, Biophysical Journal 100 (2011) 2839–2845."},"day":"08","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","intvolume":"       100","status":"public","title":"Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes","publication_status":"published","date_updated":"2021-01-12T07:42:23Z","type":"journal_article","publisher":"Biophysical Society","publist_id":"3360","extern":"1","year":"2011","acknowledgement":"Y 250-B03/Austrian Science Fund FWF/Austria","doi":"10.1016/j.bpj.2011.04.035","language":[{"iso":"eng"}],"publication":"Biophysical Journal","month":"06"},{"volume":1808,"page":"2581 - 2590","_id":"3286","date_created":"2018-12-11T12:02:28Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"date_published":"2011-10-01T00:00:00Z","citation":{"apa":"Weghuber, J., Aichinger, M., Brameshuber, M., Wieser, S., Ruprecht, V., Plochberger, B., … Schuetz, G. (2011). Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells. <i>Biochimica et Biophysica Acta (BBA) - Biomembranes</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bbamem.2011.06.007\">https://doi.org/10.1016/j.bbamem.2011.06.007</a>","ama":"Weghuber J, Aichinger M, Brameshuber M, et al. Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells. <i>Biochimica et Biophysica Acta (BBA) - Biomembranes</i>. 2011;1808(10):2581-2590. doi:<a href=\"https://doi.org/10.1016/j.bbamem.2011.06.007\">10.1016/j.bbamem.2011.06.007</a>","mla":"Weghuber, Julian, et al. “Cationic Amphipathic Peptides Accumulate Sialylated Proteins and Lipids in the Plasma Membrane of Eukaryotic Host Cells.” <i>Biochimica et Biophysica Acta (BBA) - Biomembranes</i>, vol. 1808, no. 10, Elsevier, 2011, pp. 2581–90, doi:<a href=\"https://doi.org/10.1016/j.bbamem.2011.06.007\">10.1016/j.bbamem.2011.06.007</a>.","ista":"Weghuber J, Aichinger M, Brameshuber M, Wieser S, Ruprecht V, Plochberger B, Madl J, Horner A, Reipert S, Lohner K, Henics T, Schuetz G. 2011. Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1808(10), 2581–2590.","short":"J. Weghuber, M. Aichinger, M. Brameshuber, S. Wieser, V. Ruprecht, B. Plochberger, J. Madl, A. Horner, S. Reipert, K. Lohner, T. Henics, G. Schuetz, Biochimica et Biophysica Acta (BBA) - Biomembranes 1808 (2011) 2581–2590.","chicago":"Weghuber, Julian, Michael Aichinger, Mario Brameshuber, Stefan Wieser, Verena Ruprecht, Birgit Plochberger, Josef Madl, et al. “Cationic Amphipathic Peptides Accumulate Sialylated Proteins and Lipids in the Plasma Membrane of Eukaryotic Host Cells.” <i>Biochimica et Biophysica Acta (BBA) - Biomembranes</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.bbamem.2011.06.007\">https://doi.org/10.1016/j.bbamem.2011.06.007</a>.","ieee":"J. Weghuber <i>et al.</i>, “Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells,” <i>Biochimica et Biophysica Acta (BBA) - Biomembranes</i>, vol. 1808, no. 10. Elsevier, pp. 2581–2590, 2011."},"day":"01","issue":"10","author":[{"last_name":"Weghuber","first_name":"Julian","full_name":"Weghuber, Julian"},{"first_name":"Michael","last_name":"Aichinger","full_name":"Aichinger, Michael C."},{"full_name":"Brameshuber, Mario","first_name":"Mario","last_name":"Brameshuber"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","orcid":"0000-0002-2670-2217","last_name":"Wieser","full_name":"Stefan Wieser"},{"last_name":"Ruprecht","orcid":"0000-0003-4088-8633","first_name":"Verena","full_name":"Verena Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Plochberger, Birgit","last_name":"Plochberger","first_name":"Birgit"},{"first_name":"Josef","last_name":"Madl","full_name":"Madl, Josef"},{"full_name":"Horner, Andreas","first_name":"Andreas","last_name":"Horner"},{"last_name":"Reipert","first_name":"Siegfried","full_name":"Reipert, Siegfried"},{"first_name":"Karl","last_name":"Lohner","full_name":"Lohner, Karl"},{"last_name":"Henics","first_name":"Tamas","full_name":"Henics, Tamas"},{"full_name":"Schuetz, Gerhard J","last_name":"Schuetz","first_name":"Gerhard"}],"quality_controlled":0,"abstract":[{"text":"Cationic antimicrobial peptides (CAMPs) selectively target bacterial membranes by electrostatic interactions with negatively charged lipids. It turned out that for inhibition of microbial growth a high CAMP membrane concentration is required, which can be realized by the incorporation of hydrophobic groups within the peptide. Increasing hydrophobicity, however, reduces the CAMP selectivity for bacterial over eukaryotic host membranes, thereby causing the risk of detrimental side-effects. In this study we addressed how cationic amphipathic peptides—in particular a CAMP with Lysine–Leucine–Lysine repeats (termed KLK)—affect the localization and dynamics of molecules in eukaryotic membranes. We found KLK to selectively inhibit the endocytosis of a subgroup of membrane proteins and lipids by electrostatically interacting with negatively charged sialic acid moieties. Ultrastructural characterization revealed the formation of membrane invaginations representing fission or fusion intermediates, in which the sialylated proteins and lipids were immobilized. Experiments on structurally different cationic amphipathic peptides (KLK, 6-MO-LF11-322 and NK14-2) indicated a cooperation of electrostatic and hydrophobic forces that selectively arrest sialylated membrane constituents.","lang":"eng"}],"type":"journal_article","date_updated":"2021-01-12T07:42:24Z","publisher":"Elsevier","publication_status":"published","title":"Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells","intvolume":"      1808","status":"public","publication":"Biochimica et Biophysica Acta (BBA) - Biomembranes","month":"10","doi":"10.1016/j.bbamem.2011.06.007","acknowledgement":"This work was funded by the GEN-AU project of the Austrian Research Promotion Agency, the Austrian Science Fund (FWF; project Y250-B03) and Intercell AG.\nWe thank the following colleagues for providing plasmids and cells: Daniel Legler (University of Konstanz, Switzerland), Jennifer Lippincott-Schwartz (NIH, Bethesda, USA), Hannes Stockinger (Medical University Vienna, Austria), Katharina Strub (University of Geneva, Switzerland), Lawrence Rajendran (ETH Zurich, Switzerland), Eileen M. Lafer (UTHSC San Antonio, Texas, USA), Mark McNiven (Mayo Clinic, Minnesota, USA), John Silvius (McGill University, Montreal, Canada), Christoph Romanin (JKU Linz, Austria), Herbert Stangl (Medical University Vienna, Austria) and Anton van der Merwe (Oxford University, Oxford, UK). We thank Harald Kotisch (MFPL, Vienna) for excellent technical assistance in the processing of samples for electron microscopy and Sergio Grinstein (Hospital for Sick Children Research Institute, Toronto) for fruitful discussions. ","extern":1,"year":"2011","publist_id":"3359"},{"language":[{"iso":"eng"}],"publication":"Current Protein & Peptide Science","month":"12","doi":"10.2174/138920311798841753","publist_id":"3358","year":"2011","type":"journal_article","date_updated":"2021-01-12T07:42:24Z","publisher":"Bentham Science Publishers","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"What can we learn from single molecule trajectories?","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"intvolume":"        12","status":"public","citation":{"ieee":"V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from single molecule trajectories?,” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.","chicago":"Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers, 2011. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>.","short":"V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein &#38; Peptide Science 12 (2011) 714–724.","ista":"Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single molecule trajectories? Current Protein &#38; Peptide Science. 12(8), 714–724.","apa":"Ruprecht, V., Axmann, M., Wieser, S., &#38; Schuetz, G. (2011). What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>","ama":"Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. 2011;12(8):714-724. doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>","mla":"Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8, Bentham Science Publishers, 2011, pp. 714–24, doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>."},"day":"01","issue":"8","author":[{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena"},{"full_name":"Axmann, Markus","last_name":"Axmann","first_name":"Markus"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","first_name":"Stefan"},{"full_name":"Schuetz, Gerhard","last_name":"Schuetz","first_name":"Gerhard"}],"abstract":[{"lang":"eng","text":"Diffusing membrane constituents are constantly exposed to a variety of forces that influence their stochastic path. Single molecule experiments allow for resolving trajectories at extremely high spatial and temporal accuracy, thereby offering insights into en route interactions of the tracer. In this review we discuss approaches to derive information about the underlying processes, based on single molecule tracking experiments. In particular, we focus on a new versatile way to analyze single molecule diffusion in the absence of a full analytical treatment. The method is based on comprehensive comparison of an experimental data set against the hypothetical outcome of multiple experiments performed on the computer. Since Monte Carlo simulations can be easily and rapidly performed even on state-of-the-art PCs, our method provides a simple way for testing various - even complicated - diffusion models. We describe the new method in detail, and show the applicability on two specific examples: firstly, kinetic rate constants can be derived for the transient interaction of mobile membrane proteins; secondly, residence time and corral size can be extracted for confined diffusion."}],"quality_controlled":"1","oa_version":"None","volume":12,"page":"714 - 724","_id":"3287","date_created":"2018-12-11T12:02:28Z","date_published":"2011-12-01T00:00:00Z","scopus_import":1}]