---
_id: '10703'
abstract:
- lang: eng
  text: 'When crawling through the body, leukocytes often traverse tissues that are
    densely packed with extracellular matrix and other cells, and this raises the
    question: How do leukocytes overcome compressive mechanical loads? Here, we show
    that the actin cortex of leukocytes is mechanoresponsive and that this responsiveness
    requires neither force sensing via the nucleus nor adhesive interactions with
    a substrate. Upon global compression of the cell body as well as local indentation
    of the plasma membrane, Wiskott-Aldrich syndrome protein (WASp) assembles into
    dot-like structures, providing activation platforms for Arp2/3 nucleated actin
    patches. These patches locally push against the external load, which can be obstructing
    collagen fibers or other cells, and thereby create space to facilitate forward
    locomotion. We show in vitro and in vivo that this WASp function is rate limiting
    for ameboid leukocyte migration in dense but not in loose environments and is
    required for trafficking through diverse tissues such as skin and lymph nodes.'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
acknowledgement: We thank N. Darwish-Miranda, F. Leite, F.P. Assen, and A. Eichner
  for advice and help with experiments. We thank J. Renkawitz, E. Kiermaier, A. Juanes
  Garcia, and M. Avellaneda for critical reading of the manuscript. We thank M. Driscoll
  for advice on fluorescent labeling of collagen gels. This research was supported
  by the Scientific Service Units (SSUs) of IST Austria through resources provided
  by Molecular Biology Services/Lab Support Facility (LSF)/Bioimaging Facility/Electron
  Microscopy Facility. This work was funded by grants from the European Research Council
  ( CoG 724373 ) and the Austrian Science Foundation (FWF) to M.S. F.G. received funding
  from the European Union’s Horizon 2020 research and innovation program under the
  Marie Skłodowska-Curie grant agreement no. 747687.
article_processing_charge: No
article_type: original
author:
- first_name: Florian
  full_name: Gaertner, Florian
  last_name: Gaertner
- first_name: Patricia
  full_name: Reis-Rodrigues, Patricia
  last_name: Reis-Rodrigues
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Miroslav
  full_name: Hons, Miroslav
  id: 4167FE56-F248-11E8-B48F-1D18A9856A87
  last_name: Hons
  orcid: 0000-0002-6625-3348
- first_name: Juan
  full_name: Aguilera, Juan
  last_name: Aguilera
- first_name: Michael
  full_name: Riedl, Michael
  id: 3BE60946-F248-11E8-B48F-1D18A9856A87
  last_name: Riedl
  orcid: 0000-0003-4844-6311
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Saren
  full_name: Tasciyan, Saren
  id: 4323B49C-F248-11E8-B48F-1D18A9856A87
  last_name: Tasciyan
  orcid: 0000-0003-1671-393X
- first_name: Aglaja
  full_name: Kopf, Aglaja
  id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
  last_name: Kopf
  orcid: 0000-0002-2187-6656
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Gaertner F, Reis-Rodrigues P, de Vries I, et al. WASp triggers mechanosensitive
    actin patches to facilitate immune cell migration in dense tissues. <i>Developmental
    Cell</i>. 2022;57(1):47-62.e9. doi:<a href="https://doi.org/10.1016/j.devcel.2021.11.024">10.1016/j.devcel.2021.11.024</a>
  apa: Gaertner, F., Reis-Rodrigues, P., de Vries, I., Hons, M., Aguilera, J., Riedl,
    M., … Sixt, M. K. (2022). WASp triggers mechanosensitive actin patches to facilitate
    immune cell migration in dense tissues. <i>Developmental Cell</i>. Cell Press ;
    Elsevier. <a href="https://doi.org/10.1016/j.devcel.2021.11.024">https://doi.org/10.1016/j.devcel.2021.11.024</a>
  chicago: Gaertner, Florian, Patricia Reis-Rodrigues, Ingrid de Vries, Miroslav Hons,
    Juan Aguilera, Michael Riedl, Alexander F Leithner, et al. “WASp Triggers Mechanosensitive
    Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental
    Cell</i>. Cell Press ; Elsevier, 2022. <a href="https://doi.org/10.1016/j.devcel.2021.11.024">https://doi.org/10.1016/j.devcel.2021.11.024</a>.
  ieee: F. Gaertner <i>et al.</i>, “WASp triggers mechanosensitive actin patches to
    facilitate immune cell migration in dense tissues,” <i>Developmental Cell</i>,
    vol. 57, no. 1. Cell Press ; Elsevier, p. 47–62.e9, 2022.
  ista: Gaertner F, Reis-Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M, Leithner
    AF, Tasciyan S, Kopf A, Merrin J, Zheden V, Kaufmann W, Hauschild R, Sixt MK.
    2022. WASp triggers mechanosensitive actin patches to facilitate immune cell migration
    in dense tissues. Developmental Cell. 57(1), 47–62.e9.
  mla: Gaertner, Florian, et al. “WASp Triggers Mechanosensitive Actin Patches to
    Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>,
    vol. 57, no. 1, Cell Press ; Elsevier, 2022, p. 47–62.e9, doi:<a href="https://doi.org/10.1016/j.devcel.2021.11.024">10.1016/j.devcel.2021.11.024</a>.
  short: F. Gaertner, P. Reis-Rodrigues, I. de Vries, M. Hons, J. Aguilera, M. Riedl,
    A.F. Leithner, S. Tasciyan, A. Kopf, J. Merrin, V. Zheden, W. Kaufmann, R. Hauschild,
    M.K. Sixt, Developmental Cell 57 (2022) 47–62.e9.
date_created: 2022-01-30T23:01:33Z
date_published: 2022-01-10T00:00:00Z
date_updated: 2024-03-25T23:30:12Z
day: '10'
ddc:
- '570'
department:
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
- _id: BjHo
doi: 10.1016/j.devcel.2021.11.024
ec_funded: 1
external_id:
  isi:
  - '000768933800005'
  pmid:
  - '34919802'
intvolume: '        57'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
  url: https://www.sciencedirect.com/science/article/pii/S1534580721009497
month: '01'
oa: 1
oa_version: Published Version
page: 47-62.e9
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: Developmental Cell
publication_identifier:
  eissn:
  - 1878-1551
  issn:
  - 1534-5807
publication_status: published
publisher: Cell Press ; Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '12726'
    relation: dissertation_contains
    status: public
  - id: '14530'
    relation: dissertation_contains
    status: public
  - id: '12401'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: WASp triggers mechanosensitive actin patches to facilitate immune cell migration
  in dense tissues
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 57
year: '2022'
...
---
_id: '7885'
abstract:
- lang: eng
  text: Eukaryotic cells migrate by coupling the intracellular force of the actin
    cytoskeleton to the environment. While force coupling is usually mediated by transmembrane
    adhesion receptors, especially those of the integrin family, amoeboid cells such
    as leukocytes can migrate extremely fast despite very low adhesive forces1. Here
    we show that leukocytes cannot only migrate under low adhesion but can also transmit
    forces in the complete absence of transmembrane force coupling. When confined
    within three-dimensional environments, they use the topographical features of
    the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton
    follows the texture of the substrate, creating retrograde shear forces that are
    sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent
    migration are not mutually exclusive, but rather are variants of the same principle
    of coupling retrograde actin flow to the environment and thus can potentially
    operate interchangeably and simultaneously. As adhesion-free migration is independent
    of the chemical composition of the environment, it renders cells completely autonomous
    in their locomotive behaviour.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: M-Shop
acknowledgement: We thank A. Leithner and J. Renkawitz for discussion and critical
  reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic
  setups; the Bioimaging Facility of IST Austria for excellent support, as well as
  the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan,
  L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme.
  This work was supported by the European Research Council (ERC StG 281556 and CoG
  724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF
  to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476).
  F.G. received funding from the European Union’s Horizon 2020 research and innovation
  programme under the Marie Skłodowska-Curie grant agreement no. 747687.
article_processing_charge: No
article_type: original
author:
- first_name: Anne
  full_name: Reversat, Anne
  id: 35B76592-F248-11E8-B48F-1D18A9856A87
  last_name: Reversat
  orcid: 0000-0003-0666-8928
- first_name: Florian R
  full_name: Gärtner, Florian R
  id: 397A88EE-F248-11E8-B48F-1D18A9856A87
  last_name: Gärtner
  orcid: 0000-0001-6120-3723
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Julian A
  full_name: Stopp, Julian A
  id: 489E3F00-F248-11E8-B48F-1D18A9856A87
  last_name: Stopp
- first_name: Saren
  full_name: Tasciyan, Saren
  id: 4323B49C-F248-11E8-B48F-1D18A9856A87
  last_name: Tasciyan
  orcid: 0000-0003-1671-393X
- first_name: Juan L
  full_name: Aguilera Servin, Juan L
  id: 2A67C376-F248-11E8-B48F-1D18A9856A87
  last_name: Aguilera Servin
  orcid: 0000-0002-2862-8372
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Miroslav
  full_name: Hons, Miroslav
  id: 4167FE56-F248-11E8-B48F-1D18A9856A87
  last_name: Hons
  orcid: 0000-0002-6625-3348
- first_name: Matthieu
  full_name: Piel, Matthieu
  last_name: Piel
- first_name: Andrew
  full_name: Callan-Jones, Andrew
  last_name: Callan-Jones
- first_name: Raphael
  full_name: Voituriez, Raphael
  last_name: Voituriez
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental
    topography. <i>Nature</i>. 2020;582:582–585. doi:<a href="https://doi.org/10.1038/s41586-020-2283-z">10.1038/s41586-020-2283-z</a>
  apa: Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera
    Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography.
    <i>Nature</i>. Springer Nature. <a href="https://doi.org/10.1038/s41586-020-2283-z">https://doi.org/10.1038/s41586-020-2283-z</a>
  chicago: Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan,
    Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental
    Topography.” <i>Nature</i>. Springer Nature, 2020. <a href="https://doi.org/10.1038/s41586-020-2283-z">https://doi.org/10.1038/s41586-020-2283-z</a>.
  ieee: A. Reversat <i>et al.</i>, “Cellular locomotion using environmental topography,”
    <i>Nature</i>, vol. 582. Springer Nature, pp. 582–585, 2020.
  ista: Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL,
    de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK.
    2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.
  mla: Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.”
    <i>Nature</i>, vol. 582, Springer Nature, 2020, pp. 582–585, doi:<a href="https://doi.org/10.1038/s41586-020-2283-z">10.1038/s41586-020-2283-z</a>.
  short: A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera
    Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez,
    M.K. Sixt, Nature 582 (2020) 582–585.
date_created: 2020-05-24T22:01:01Z
date_published: 2020-06-25T00:00:00Z
date_updated: 2024-03-25T23:30:12Z
day: '25'
department:
- _id: NanoFab
- _id: Bio
- _id: MiSi
doi: 10.1038/s41586-020-2283-z
ec_funded: 1
external_id:
  isi:
  - '000532688300008'
intvolume: '       582'
isi: 1
language:
- iso: eng
month: '06'
oa_version: None
page: 582–585
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
- _id: 26018E70-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29911
  name: Mechanical adaptation of lamellipodial actin
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Nature
publication_identifier:
  eissn:
  - '14764687'
  issn:
  - '00280836'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/
  record:
  - id: '14697'
    relation: dissertation_contains
    status: public
  - id: '12401'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Cellular locomotion using environmental topography
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 582
year: '2020'
...
---
_id: '6328'
abstract:
- lang: eng
  text: During metazoan development, immune surveillance and cancer dissemination,
    cells migrate in complex three-dimensional microenvironments1,2,3. These spaces
    are crowded by cells and extracellular matrix, generating mazes with differently
    sized gaps that are typically smaller than the diameter of the migrating cell4,5.
    Most mesenchymal and epithelial cells and some—but not all—cancer cells actively
    generate their migratory path using pericellular tissue proteolysis6. By contrast,
    amoeboid cells such as leukocytes use non-destructive strategies of locomotion7,
    raising the question how these extremely fast cells navigate through dense tissues.
    Here we reveal that leukocytes sample their immediate vicinity for large pore
    sizes, and are thereby able to choose the path of least resistance. This allows
    them to circumnavigate local obstacles while effectively following global directional
    cues such as chemotactic gradients. Pore-size discrimination is facilitated by
    frontward positioning of the nucleus, which enables the cells to use their bulkiest
    compartment as a mechanical gauge. Once the nucleus and the closely associated
    microtubule organizing centre pass the largest pore, cytoplasmic protrusions still
    lingering in smaller pores are retracted. These retractions are coordinated by
    dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence
    and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning
    in front of the microtubule organizing centre is a typical feature of amoeboid
    migration, our findings link the fundamental organization of cellular polarity
    to the strategy of locomotion.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: letter_note
author:
- first_name: Jörg
  full_name: Renkawitz, Jörg
  id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
  last_name: Renkawitz
  orcid: 0000-0003-2856-3369
- first_name: Aglaja
  full_name: Kopf, Aglaja
  id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
  last_name: Kopf
  orcid: 0000-0002-2187-6656
- first_name: Julian A
  full_name: Stopp, Julian A
  id: 489E3F00-F248-11E8-B48F-1D18A9856A87
  last_name: Stopp
- first_name: Ingrid
  full_name: de Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: de Vries
- first_name: Meghan K.
  full_name: Driscoll, Meghan K.
  last_name: Driscoll
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Erik S.
  full_name: Welf, Erik S.
  last_name: Welf
- first_name: Gaudenz
  full_name: Danuser, Gaudenz
  last_name: Danuser
- first_name: Reto
  full_name: Fiolka, Reto
  last_name: Fiolka
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Renkawitz J, Kopf A, Stopp JA, et al. Nuclear positioning facilitates amoeboid
    migration along the path of least resistance. <i>Nature</i>. 2019;568:546-550.
    doi:<a href="https://doi.org/10.1038/s41586-019-1087-5">10.1038/s41586-019-1087-5</a>
  apa: Renkawitz, J., Kopf, A., Stopp, J. A., de Vries, I., Driscoll, M. K., Merrin,
    J., … Sixt, M. K. (2019). Nuclear positioning facilitates amoeboid migration along
    the path of least resistance. <i>Nature</i>. Springer Nature. <a href="https://doi.org/10.1038/s41586-019-1087-5">https://doi.org/10.1038/s41586-019-1087-5</a>
  chicago: Renkawitz, Jörg, Aglaja Kopf, Julian A Stopp, Ingrid de Vries, Meghan K.
    Driscoll, Jack Merrin, Robert Hauschild, et al. “Nuclear Positioning Facilitates
    Amoeboid Migration along the Path of Least Resistance.” <i>Nature</i>. Springer
    Nature, 2019. <a href="https://doi.org/10.1038/s41586-019-1087-5">https://doi.org/10.1038/s41586-019-1087-5</a>.
  ieee: J. Renkawitz <i>et al.</i>, “Nuclear positioning facilitates amoeboid migration
    along the path of least resistance,” <i>Nature</i>, vol. 568. Springer Nature,
    pp. 546–550, 2019.
  ista: Renkawitz J, Kopf A, Stopp JA, de Vries I, Driscoll MK, Merrin J, Hauschild
    R, Welf ES, Danuser G, Fiolka R, Sixt MK. 2019. Nuclear positioning facilitates
    amoeboid migration along the path of least resistance. Nature. 568, 546–550.
  mla: Renkawitz, Jörg, et al. “Nuclear Positioning Facilitates Amoeboid Migration
    along the Path of Least Resistance.” <i>Nature</i>, vol. 568, Springer Nature,
    2019, pp. 546–50, doi:<a href="https://doi.org/10.1038/s41586-019-1087-5">10.1038/s41586-019-1087-5</a>.
  short: J. Renkawitz, A. Kopf, J.A. Stopp, I. de Vries, M.K. Driscoll, J. Merrin,
    R. Hauschild, E.S. Welf, G. Danuser, R. Fiolka, M.K. Sixt, Nature 568 (2019) 546–550.
date_created: 2019-04-17T06:52:28Z
date_published: 2019-04-25T00:00:00Z
date_updated: 2024-03-25T23:30:22Z
day: '25'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/s41586-019-1087-5
ec_funded: 1
external_id:
  isi:
  - '000465594200050'
  pmid:
  - '30944468'
intvolume: '       568'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217284/
month: '04'
oa: 1
oa_version: Submitted Version
page: 546-550
pmid: 1
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
- _id: 265FAEBA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W01250-B20
  name: Nano-Analytics of Cellular Systems
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 25A48D24-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 1396-2014
  name: Molecular and system level view of immune cell migration
publication: Nature
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/leukocytes-use-their-nucleus-as-a-ruler-to-choose-path-of-least-resistance/
  record:
  - id: '14697'
    relation: dissertation_contains
    status: public
  - id: '6891'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Nuclear positioning facilitates amoeboid migration along the path of least
  resistance
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 568
year: '2019'
...
---
_id: '6497'
abstract:
- lang: eng
  text: T cells are actively scanning pMHC-presenting cells in lymphoid organs and
    nonlymphoid tissues (NLTs) with divergent topologies and confinement. How the
    T cell actomyosin cytoskeleton facilitates this task in distinct environments
    is incompletely understood. Here, we show that lack of Myosin IXb (Myo9b), a negative
    regulator of the small GTPase Rho, led to increased Rho-GTP levels and cell surface
    stiffness in primary T cells. Nonetheless, intravital imaging revealed robust
    motility of Myo9b−/− CD8+ T cells in lymphoid tissue and similar expansion and
    differentiation during immune responses. In contrast, accumulation of Myo9b−/−
    CD8+ T cells in NLTs was strongly impaired. Specifically, Myo9b was required for
    T cell crossing of basement membranes, such as those which are present between
    dermis and epidermis. As consequence, Myo9b−/− CD8+ T cells showed impaired control
    of skin infections. In sum, we show that Myo9b is critical for the CD8+ T cell
    adaptation from lymphoid to NLT surveillance and the establishment of protective
    tissue–resident T cell populations.
article_processing_charge: No
author:
- first_name: Federica
  full_name: Moalli, Federica
  last_name: Moalli
- first_name: Xenia
  full_name: Ficht, Xenia
  last_name: Ficht
- first_name: Philipp
  full_name: Germann, Philipp
  last_name: Germann
- first_name: Mykhailo
  full_name: Vladymyrov, Mykhailo
  last_name: Vladymyrov
- first_name: Bettina
  full_name: Stolp, Bettina
  last_name: Stolp
- first_name: Ingrid
  full_name: de Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: de Vries
- first_name: Ruth
  full_name: Lyck, Ruth
  last_name: Lyck
- first_name: Jasmin
  full_name: Balmer, Jasmin
  last_name: Balmer
- first_name: Amleto
  full_name: Fiocchi, Amleto
  last_name: Fiocchi
- first_name: Mario
  full_name: Kreutzfeldt, Mario
  last_name: Kreutzfeldt
- first_name: Doron
  full_name: Merkler, Doron
  last_name: Merkler
- first_name: Matteo
  full_name: Iannacone, Matteo
  last_name: Iannacone
- first_name: Akitaka
  full_name: Ariga, Akitaka
  last_name: Ariga
- first_name: Michael H.
  full_name: Stoffel, Michael H.
  last_name: Stoffel
- first_name: James
  full_name: Sharpe, James
  last_name: Sharpe
- first_name: Martin
  full_name: Bähler, Martin
  last_name: Bähler
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Alba
  full_name: Diz-Muñoz, Alba
  last_name: Diz-Muñoz
- first_name: Jens V.
  full_name: Stein, Jens V.
  last_name: Stein
citation:
  ama: Moalli F, Ficht X, Germann P, et al. The Rho regulator Myosin IXb enables nonlymphoid
    tissue seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>.
    2018;2015(7):1869–1890. doi:<a href="https://doi.org/10.1084/jem.20170896">10.1084/jem.20170896</a>
  apa: Moalli, F., Ficht, X., Germann, P., Vladymyrov, M., Stolp, B., de Vries, I.,
    … Stein, J. V. (2018). The Rho regulator Myosin IXb enables nonlymphoid tissue
    seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>.
    Rockefeller University Press. <a href="https://doi.org/10.1084/jem.20170896">https://doi.org/10.1084/jem.20170896</a>
  chicago: Moalli, Federica, Xenia Ficht, Philipp Germann, Mykhailo Vladymyrov, Bettina
    Stolp, Ingrid de Vries, Ruth Lyck, et al. “The Rho Regulator Myosin IXb Enables
    Nonlymphoid Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental
    Medicine</i>. Rockefeller University Press, 2018. <a href="https://doi.org/10.1084/jem.20170896">https://doi.org/10.1084/jem.20170896</a>.
  ieee: F. Moalli <i>et al.</i>, “The Rho regulator Myosin IXb enables nonlymphoid
    tissue seeding of protective CD8+T cells,” <i>The Journal of Experimental Medicine</i>,
    vol. 2015, no. 7. Rockefeller University Press, pp. 1869–1890, 2018.
  ista: Moalli F, Ficht X, Germann P, Vladymyrov M, Stolp B, de Vries I, Lyck R, Balmer
    J, Fiocchi A, Kreutzfeldt M, Merkler D, Iannacone M, Ariga A, Stoffel MH, Sharpe
    J, Bähler M, Sixt MK, Diz-Muñoz A, Stein JV. 2018. The Rho regulator Myosin IXb
    enables nonlymphoid tissue seeding of protective CD8+T cells. The Journal of Experimental
    Medicine. 2015(7), 1869–1890.
  mla: Moalli, Federica, et al. “The Rho Regulator Myosin IXb Enables Nonlymphoid
    Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental Medicine</i>,
    vol. 2015, no. 7, Rockefeller University Press, 2018, pp. 1869–1890, doi:<a href="https://doi.org/10.1084/jem.20170896">10.1084/jem.20170896</a>.
  short: F. Moalli, X. Ficht, P. Germann, M. Vladymyrov, B. Stolp, I. de Vries, R.
    Lyck, J. Balmer, A. Fiocchi, M. Kreutzfeldt, D. Merkler, M. Iannacone, A. Ariga,
    M.H. Stoffel, J. Sharpe, M. Bähler, M.K. Sixt, A. Diz-Muñoz, J.V. Stein, The Journal
    of Experimental Medicine 2015 (2018) 1869–1890.
date_created: 2019-05-28T12:36:47Z
date_published: 2018-06-06T00:00:00Z
date_updated: 2023-09-19T14:52:08Z
day: '06'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1084/jem.20170896
external_id:
  isi:
  - '000440822900011'
file:
- access_level: open_access
  checksum: 86ae5331f9bfced9a6358a790a04bef4
  content_type: application/pdf
  creator: kschuh
  date_created: 2019-05-28T12:40:05Z
  date_updated: 2020-07-14T12:47:32Z
  file_id: '6498'
  file_name: 2018_rupress_Moalli.pdf
  file_size: 3841660
  relation: main_file
file_date_updated: 2020-07-14T12:47:32Z
has_accepted_license: '1'
intvolume: '      2015'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '06'
oa: 1
oa_version: Published Version
page: 1869–1890
publication: The Journal of Experimental Medicine
publication_identifier:
  eissn:
  - 1540-9538
  issn:
  - 0022-1007
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective
  CD8+T cells
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
    BY-NC-SA 4.0)
  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 2015
year: '2018'
...
---
_id: '437'
abstract:
- lang: eng
  text: Dendritic cells (DCs) are sentinels of the adaptive immune system that reside
    in peripheral organs of mammals. Upon pathogen encounter, they undergo maturation
    and up-regulate the chemokine receptor CCR7 that guides them along gradients of
    its chemokine ligands CCL19 and 21 to the next draining lymph node. There, DCs
    present peripherally acquired antigen to naïve T cells, thereby triggering adaptive
    immunity.
acknowledged_ssus:
- _id: SSU
acknowledgement: "This work was supported by grants of the European Research Council
  (ERC CoG 724373) and the Austrian Science Fund (FWF) to M.S. We thank the scientific
  support units at IST Austria for excellent technical support.\r\nWe thank the  scientific
  \ support units at IST Austria for excellent technical support.   "
article_processing_charge: Yes (via OA deal)
author:
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Jörg
  full_name: Renkawitz, Jörg
  id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
  last_name: Renkawitz
  orcid: 0000-0003-2856-3369
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Hans
  full_name: Haecker, Hans
  last_name: Haecker
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Leithner AF, Renkawitz J, de Vries I, Hauschild R, Haecker H, Sixt MK. Fast
    and efficient genetic engineering of hematopoietic precursor cells for the study
    of dendritic cell migration. <i>European Journal of Immunology</i>. 2018;48(6):1074-1077.
    doi:<a href="https://doi.org/10.1002/eji.201747358">10.1002/eji.201747358</a>
  apa: Leithner, A. F., Renkawitz, J., de Vries, I., Hauschild, R., Haecker, H., &#38;
    Sixt, M. K. (2018). Fast and efficient genetic engineering of hematopoietic precursor
    cells for the study of dendritic cell migration. <i>European Journal of Immunology</i>.
    Wiley-Blackwell. <a href="https://doi.org/10.1002/eji.201747358">https://doi.org/10.1002/eji.201747358</a>
  chicago: Leithner, Alexander F, Jörg Renkawitz, Ingrid de Vries, Robert Hauschild,
    Hans Haecker, and Michael K Sixt. “Fast and Efficient Genetic Engineering of Hematopoietic
    Precursor Cells for the Study of Dendritic Cell Migration.” <i>European Journal
    of Immunology</i>. Wiley-Blackwell, 2018. <a href="https://doi.org/10.1002/eji.201747358">https://doi.org/10.1002/eji.201747358</a>.
  ieee: A. F. Leithner, J. Renkawitz, I. de Vries, R. Hauschild, H. Haecker, and M.
    K. Sixt, “Fast and efficient genetic engineering of hematopoietic precursor cells
    for the study of dendritic cell migration,” <i>European Journal of Immunology</i>,
    vol. 48, no. 6. Wiley-Blackwell, pp. 1074–1077, 2018.
  ista: Leithner AF, Renkawitz J, de Vries I, Hauschild R, Haecker H, Sixt MK. 2018.
    Fast and efficient genetic engineering of hematopoietic precursor cells for the
    study of dendritic cell migration. European Journal of Immunology. 48(6), 1074–1077.
  mla: Leithner, Alexander F., et al. “Fast and Efficient Genetic Engineering of Hematopoietic
    Precursor Cells for the Study of Dendritic Cell Migration.” <i>European Journal
    of Immunology</i>, vol. 48, no. 6, Wiley-Blackwell, 2018, pp. 1074–77, doi:<a
    href="https://doi.org/10.1002/eji.201747358">10.1002/eji.201747358</a>.
  short: A.F. Leithner, J. Renkawitz, I. de Vries, R. Hauschild, H. Haecker, M.K.
    Sixt, European Journal of Immunology 48 (2018) 1074–1077.
date_created: 2018-12-11T11:46:28Z
date_published: 2018-02-13T00:00:00Z
date_updated: 2023-09-11T14:01:18Z
day: '13'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
doi: 10.1002/eji.201747358
ec_funded: 1
external_id:
  isi:
  - '000434963700016'
file:
- access_level: open_access
  checksum: 9d5b74cd016505aeb9a4c2d33bbedaeb
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:56Z
  date_updated: 2020-07-14T12:46:27Z
  file_id: '5044'
  file_name: IST-2018-1067-v1+2_Leithner_et_al-2018-European_Journal_of_Immunology.pdf
  file_size: 590106
  relation: main_file
file_date_updated: 2020-07-14T12:46:27Z
has_accepted_license: '1'
intvolume: '        48'
isi: 1
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: 1074 - 1077
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: European Journal of Immunology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '7386'
pubrep_id: '1067'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fast and efficient genetic engineering of hematopoietic precursor cells for
  the study of dendritic cell migration
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 48
year: '2018'
...
---
_id: '672'
abstract:
- lang: eng
  text: Trafficking cells frequently transmigrate through epithelial and endothelial
    monolayers. How monolayers cooperate with the penetrating cells to support their
    transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic
    capillaries as a model system for transendothelial migration. We find that the
    chemokine CCL21, which is the decisive guidance cue for intravasation, mainly
    localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial
    cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes
    extracellularly enriched at the sites of endothelial cell-cell junctions. When
    we reconstitute the transmigration process in vitro, we find that secretion of
    CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and
    selective calcium chelation in lymphatic endothelium attenuates transmigration.
    Altogether, our data demonstrate a chemokine-mediated feedback between DCs and
    lymphatic endothelium, which facilitates transendothelial migration.
article_processing_charge: Yes
author:
- first_name: Kari
  full_name: Vaahtomeri, Kari
  id: 368EE576-F248-11E8-B48F-1D18A9856A87
  last_name: Vaahtomeri
  orcid: 0000-0001-7829-3518
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
- first_name: Matthias
  full_name: Mehling, Matthias
  id: 3C23B994-F248-11E8-B48F-1D18A9856A87
  last_name: Mehling
  orcid: 0000-0001-8599-1226
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Vaahtomeri K, Brown M, Hauschild R, et al. Locally triggered release of the
    chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia.
    <i>Cell Reports</i>. 2017;19(5):902-909. doi:<a href="https://doi.org/10.1016/j.celrep.2017.04.027">10.1016/j.celrep.2017.04.027</a>
  apa: Vaahtomeri, K., Brown, M., Hauschild, R., de Vries, I., Leithner, A. F., Mehling,
    M., … Sixt, M. K. (2017). Locally triggered release of the chemokine CCL21 promotes
    dendritic cell transmigration across lymphatic endothelia. <i>Cell Reports</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.celrep.2017.04.027">https://doi.org/10.1016/j.celrep.2017.04.027</a>
  chicago: Vaahtomeri, Kari, Markus Brown, Robert Hauschild, Ingrid de Vries, Alexander
    F Leithner, Matthias Mehling, Walter Kaufmann, and Michael K Sixt. “Locally Triggered
    Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic
    Endothelia.” <i>Cell Reports</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.celrep.2017.04.027">https://doi.org/10.1016/j.celrep.2017.04.027</a>.
  ieee: K. Vaahtomeri <i>et al.</i>, “Locally triggered release of the chemokine CCL21
    promotes dendritic cell transmigration across lymphatic endothelia,” <i>Cell Reports</i>,
    vol. 19, no. 5. Cell Press, pp. 902–909, 2017.
  ista: Vaahtomeri K, Brown M, Hauschild R, de Vries I, Leithner AF, Mehling M, Kaufmann
    W, Sixt MK. 2017. Locally triggered release of the chemokine CCL21 promotes dendritic
    cell transmigration across lymphatic endothelia. Cell Reports. 19(5), 902–909.
  mla: Vaahtomeri, Kari, et al. “Locally Triggered Release of the Chemokine CCL21
    Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” <i>Cell Reports</i>,
    vol. 19, no. 5, Cell Press, 2017, pp. 902–09, doi:<a href="https://doi.org/10.1016/j.celrep.2017.04.027">10.1016/j.celrep.2017.04.027</a>.
  short: K. Vaahtomeri, M. Brown, R. Hauschild, I. de Vries, A.F. Leithner, M. Mehling,
    W. Kaufmann, M.K. Sixt, Cell Reports 19 (2017) 902–909.
date_created: 2018-12-11T11:47:50Z
date_published: 2017-05-02T00:00:00Z
date_updated: 2023-02-23T12:50:09Z
day: '02'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
- _id: EM-Fac
doi: 10.1016/j.celrep.2017.04.027
ec_funded: 1
file:
- access_level: open_access
  checksum: 8fdddaab1f1d76a6ec9ca94dcb6b07a2
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:14:54Z
  date_updated: 2020-07-14T12:47:38Z
  file_id: '5109'
  file_name: IST-2017-900-v1+1_1-s2.0-S2211124717305211-main.pdf
  file_size: 2248814
  relation: main_file
file_date_updated: 2020-07-14T12:47:38Z
has_accepted_license: '1'
intvolume: '        19'
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 902 - 909
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and transduction of leukocytes (FWF)
publication: Cell Reports
publication_identifier:
  issn:
  - '22111247'
publication_status: published
publisher: Cell Press
publist_id: '7052'
pubrep_id: '900'
quality_controlled: '1'
scopus_import: 1
status: public
title: Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration
  across lymphatic endothelia
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2017'
...
---
_id: '674'
abstract:
- lang: eng
  text: Navigation of cells along gradients of guidance cues is a determining step
    in many developmental and immunological processes. Gradients can either be soluble
    or immobilized to tissues as demonstrated for the haptotactic migration of dendritic
    cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate
    how gradient characteristics govern cellular response patterns, we here introduce
    an in vitro system allowing to track migratory responses of DCs to precisely controlled
    immobilized gradients of CCL21. We find that haptotactic sensing depends on the
    absolute CCL21 concentration and local steepness of the gradient, consistent with
    a scenario where DC directionality is governed by the signal-to-noise ratio of
    CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC
    guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore,
    we find that CCR7 signal termination by the G-protein-coupled receptor kinase
    6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient
    sensing in vitro and confirm those observations in vivo. These findings suggest
    that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal
    guidance in vivo.
author:
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Veronika
  full_name: Bierbaum, Veronika
  id: 3FD04378-F248-11E8-B48F-1D18A9856A87
  last_name: Bierbaum
- first_name: Kari
  full_name: Vaahtomeri, Kari
  id: 368EE576-F248-11E8-B48F-1D18A9856A87
  last_name: Vaahtomeri
  orcid: 0000-0001-7829-3518
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
- first_name: Anne
  full_name: Reversat, Anne
  id: 35B76592-F248-11E8-B48F-1D18A9856A87
  last_name: Reversat
  orcid: 0000-0003-0666-8928
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Teresa
  full_name: Tarrant, Teresa
  last_name: Tarrant
- first_name: Tobias
  full_name: Bollenbach, Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic
    chemokine gradients in a manner governed by signal to noise ratio and dependent
    on GRK6. <i>Current Biology</i>. 2017;27(9):1314-1325. doi:<a href="https://doi.org/10.1016/j.cub.2017.04.004">10.1016/j.cub.2017.04.004</a>
  apa: Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries,
    I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients
    in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current
    Biology</i>. Cell Press. <a href="https://doi.org/10.1016/j.cub.2017.04.004">https://doi.org/10.1016/j.cub.2017.04.004</a>
  chicago: Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus
    Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret
    Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio
    and Dependent on GRK6.” <i>Current Biology</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cub.2017.04.004">https://doi.org/10.1016/j.cub.2017.04.004</a>.
  ieee: J. Schwarz <i>et al.</i>, “Dendritic cells interpret haptotactic chemokine
    gradients in a manner governed by signal to noise ratio and dependent on GRK6,”
    <i>Current Biology</i>, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017.
  ista: Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner
    AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells
    interpret haptotactic chemokine gradients in a manner governed by signal to noise
    ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.
  mla: Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients
    in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current
    Biology</i>, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:<a href="https://doi.org/10.1016/j.cub.2017.04.004">10.1016/j.cub.2017.04.004</a>.
  short: J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries,
    A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt,
    Current Biology 27 (2017) 1314–1325.
date_created: 2018-12-11T11:47:51Z
date_published: 2017-05-09T00:00:00Z
date_updated: 2023-02-23T12:50:44Z
day: '09'
department:
- _id: MiSi
- _id: Bio
- _id: NanoFab
doi: 10.1016/j.cub.2017.04.004
ec_funded: 1
intvolume: '        27'
issue: '9'
language:
- iso: eng
month: '05'
oa_version: None
page: 1314 - 1325
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and transduction of leukocytes (FWF)
publication: Current Biology
publication_identifier:
  issn:
  - '09609822'
publication_status: published
publisher: Cell Press
publist_id: '7050'
quality_controlled: '1'
scopus_import: 1
status: public
title: Dendritic cells interpret haptotactic chemokine gradients in a manner governed
  by signal to noise ratio and dependent on GRK6
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2017'
...
---
_id: '727'
abstract:
- lang: eng
  text: 'Actin filaments polymerizing against membranes power endocytosis, vesicular
    traffic, and cell motility. In vitro reconstitution studies suggest that the structure
    and the dynamics of actin networks respond to mechanical forces. We demonstrate
    that lamellipodial actin of migrating cells responds to mechanical load when membrane
    tension is modulated. In a steady state, migrating cell filaments assume the canonical
    dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension
    triggers a dense network with a broadened range of angles, whereas decreased tension
    causes a shift to a sparse configuration dominated by filaments growing perpendicularly
    to the plasma membrane. We show that these responses emerge from the geometry
    of branched actin: when load per filament decreases, elongation speed increases
    and perpendicular filaments gradually outcompete others because they polymerize
    the shortest distance to the membrane, where they are protected from capping.
    This network-intrinsic geometrical adaptation mechanism tunes protrusive force
    in response to mechanical load.'
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: Jan
  full_name: Mueller, Jan
  last_name: Mueller
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Arnon
  full_name: Lieber, Arnon
  last_name: Lieber
- first_name: Christoph
  full_name: Winkler, Christoph
  last_name: Winkler
- first_name: Karsten
  full_name: Kruse, Karsten
  last_name: Kruse
- first_name: John
  full_name: Small, John
  last_name: Small
- first_name: Christian
  full_name: Schmeiser, Christian
  last_name: Schmeiser
- first_name: Kinneret
  full_name: Keren, Kinneret
  last_name: Keren
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin
    networks. <i>Cell</i>. 2017;171(1):188-200. doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>
  apa: Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C.,
    … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. <i>Cell</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>
  chicago: Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber,
    Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin
    Networks.” <i>Cell</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>.
  ieee: J. Mueller <i>et al.</i>, “Load adaptation of lamellipodial actin networks,”
    <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.
  ista: Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K,
    Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of
    lamellipodial actin networks. Cell. 171(1), 188–200.
  mla: Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>,
    vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>.
  short: J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K.
    Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017)
    188–200.
date_created: 2018-12-11T11:48:10Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2023-09-28T11:33:49Z
day: '21'
department:
- _id: MiSi
- _id: Bio
doi: 10.1016/j.cell.2017.07.051
ec_funded: 1
external_id:
  isi:
  - '000411331800020'
intvolume: '       171'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
page: 188 - 200
project:
- _id: 25AD6156-B435-11E9-9278-68D0E5697425
  grant_number: LS13-029
  name: Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
publication: Cell
publication_identifier:
  issn:
  - '00928674'
publication_status: published
publisher: Cell Press
publist_id: '6951'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Load adaptation of lamellipodial actin networks
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 171
year: '2017'
...
---
_id: '1599'
abstract:
- lang: eng
  text: "The addition of polysialic acid to N- and/or O-linked glycans, referred to
    as polysialylation, is a rare posttranslational modification that is mainly known
    to control the developmental plasticity of the nervous system. Here we show that
    CCR7, the central chemokine receptor controlling immune cell trafficking to secondary
    lymphatic organs, carries polysialic acid. This modification is essential for
    the recognition of the CCR7 ligand CCL21. As a consequence, dendritic cell trafficking
    is abrogated in polysialyltransferase-deficient mice, manifesting as disturbed
    lymph node homeostasis and unresponsiveness to inflammatory stimuli. Structure-function
    analysis of chemokine-receptor interactions reveals that CCL21 adopts an autoinhibited
    conformation, which is released upon interaction with polysialic acid. Thus, we
    describe a glycosylation-mediated immune cell trafficking disorder and its mechanistic
    basis.\r\n"
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We thank S. Schüchner and E. Ogris for kindly providing the antibody
  to GFP, M. Helmbrecht and A. Huber for providing Nrp2−/− mice, the IST Scientific
  Support Facilities for excellent services, and J. Renkawitz and K. Vaahtomeri for
  critically reading the manuscript. '
article_processing_charge: No
article_type: original
author:
- first_name: Eva
  full_name: Kiermaier, Eva
  id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
  last_name: Kiermaier
  orcid: 0000-0001-6165-5738
- first_name: Christine
  full_name: Moussion, Christine
  id: 3356F664-F248-11E8-B48F-1D18A9856A87
  last_name: Moussion
- first_name: Christopher
  full_name: Veldkamp, Christopher
  last_name: Veldkamp
- first_name: Rita
  full_name: Gerardy  Schahn, Rita
  last_name: Gerardy  Schahn
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Larry
  full_name: Williams, Larry
  last_name: Williams
- first_name: Gary
  full_name: Chaffee, Gary
  last_name: Chaffee
- first_name: Andrew
  full_name: Phillips, Andrew
  last_name: Phillips
- first_name: Friedrich
  full_name: Freiberger, Friedrich
  last_name: Freiberger
- first_name: Richard
  full_name: Imre, Richard
  last_name: Imre
- first_name: Deni
  full_name: Taleski, Deni
  last_name: Taleski
- first_name: Richard
  full_name: Payne, Richard
  last_name: Payne
- first_name: Asolina
  full_name: Braun, Asolina
  last_name: Braun
- first_name: Reinhold
  full_name: Förster, Reinhold
  last_name: Förster
- first_name: Karl
  full_name: Mechtler, Karl
  last_name: Mechtler
- first_name: Martina
  full_name: Mühlenhoff, Martina
  last_name: Mühlenhoff
- first_name: Brian
  full_name: Volkman, Brian
  last_name: Volkman
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Kiermaier E, Moussion C, Veldkamp C, et al. Polysialylation controls dendritic
    cell trafficking by regulating chemokine recognition. <i>Science</i>. 2016;351(6269):186-190.
    doi:<a href="https://doi.org/10.1126/science.aad0512">10.1126/science.aad0512</a>
  apa: Kiermaier, E., Moussion, C., Veldkamp, C., Gerardy  Schahn, R., de Vries, I.,
    Williams, L., … Sixt, M. K. (2016). Polysialylation controls dendritic cell trafficking
    by regulating chemokine recognition. <i>Science</i>. American Association for
    the Advancement of Science. <a href="https://doi.org/10.1126/science.aad0512">https://doi.org/10.1126/science.aad0512</a>
  chicago: Kiermaier, Eva, Christine Moussion, Christopher Veldkamp, Rita Gerardy 
    Schahn, Ingrid de Vries, Larry Williams, Gary Chaffee, et al. “Polysialylation
    Controls Dendritic Cell Trafficking by Regulating Chemokine Recognition.” <i>Science</i>.
    American Association for the Advancement of Science, 2016. <a href="https://doi.org/10.1126/science.aad0512">https://doi.org/10.1126/science.aad0512</a>.
  ieee: E. Kiermaier <i>et al.</i>, “Polysialylation controls dendritic cell trafficking
    by regulating chemokine recognition,” <i>Science</i>, vol. 351, no. 6269. American
    Association for the Advancement of Science, pp. 186–190, 2016.
  ista: Kiermaier E, Moussion C, Veldkamp C, Gerardy  Schahn R, de Vries I, Williams
    L, Chaffee G, Phillips A, Freiberger F, Imre R, Taleski D, Payne R, Braun A, Förster
    R, Mechtler K, Mühlenhoff M, Volkman B, Sixt MK. 2016. Polysialylation controls
    dendritic cell trafficking by regulating chemokine recognition. Science. 351(6269),
    186–190.
  mla: Kiermaier, Eva, et al. “Polysialylation Controls Dendritic Cell Trafficking
    by Regulating Chemokine Recognition.” <i>Science</i>, vol. 351, no. 6269, American
    Association for the Advancement of Science, 2016, pp. 186–90, doi:<a href="https://doi.org/10.1126/science.aad0512">10.1126/science.aad0512</a>.
  short: E. Kiermaier, C. Moussion, C. Veldkamp, R. Gerardy  Schahn, I. de Vries,
    L. Williams, G. Chaffee, A. Phillips, F. Freiberger, R. Imre, D. Taleski, R. Payne,
    A. Braun, R. Förster, K. Mechtler, M. Mühlenhoff, B. Volkman, M.K. Sixt, Science
    351 (2016) 186–190.
date_created: 2018-12-11T11:52:57Z
date_published: 2016-01-08T00:00:00Z
date_updated: 2021-01-12T06:51:52Z
day: '08'
department:
- _id: MiSi
doi: 10.1126/science.aad0512
ec_funded: 1
external_id:
  pmid:
  - '26657283'
intvolume: '       351'
issue: '6269'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583642/
month: '01'
oa: 1
oa_version: Submitted Version
page: 186 - 190
pmid: 1
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
- _id: 25A76F58-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '289720'
  name: Stromal Cell-immune Cell Interactions in Health and Disease
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and transduction of leukocytes (FWF)
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5570'
quality_controlled: '1'
scopus_import: 1
status: public
title: Polysialylation controls dendritic cell trafficking by regulating chemokine
  recognition
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 351
year: '2016'
...
---
_id: '1321'
abstract:
- lang: eng
  text: Most migrating cells extrude their front by the force of actin polymerization.
    Polymerization requires an initial nucleation step, which is mediated by factors
    establishing either parallel filaments in the case of filopodia or branched filaments
    that form the branched lamellipodial network. Branches are considered essential
    for regular cell motility and are initiated by the Arp2/3 complex, which in turn
    is activated by nucleation-promoting factors of the WASP and WAVE families. Here
    we employed rapid amoeboid crawling leukocytes and found that deletion of the
    WAVE complex eliminated actin branching and thus lamellipodia formation. The cells
    were left with parallel filaments at the leading edge, which translated, depending
    on the differentiation status of the cell, into a unipolar pointed cell shape
    or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased
    speed and enormous directional persistence, while they were unable to turn towards
    chemotactic gradients. Cells with multiple filopodia retained chemotactic activity
    but their migration was progressively impaired with increasing geometrical complexity
    of the extracellular environment. These findings establish that diversified leading
    edge protrusions serve as explorative structures while they slow down actual locomotion.
acknowledged_ssus:
- _id: SSU
acknowledgement: "This work was supported by the German Research Foundation (DFG)
  Priority Program SP 1464 to T.E.B.S. and M.S., and European Research Council (ERC
  GA 281556) and Human Frontiers Program grants to M.S.\r\nService Units of IST Austria
  for excellent technical support."
article_processing_charge: No
article_type: original
author:
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Alexander
  full_name: Eichner, Alexander
  id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
  last_name: Eichner
- first_name: Jan
  full_name: Müller, Jan
  id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
  last_name: Müller
- first_name: Anne
  full_name: Reversat, Anne
  id: 35B76592-F248-11E8-B48F-1D18A9856A87
  last_name: Reversat
  orcid: 0000-0003-0666-8928
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: David
  full_name: De Gorter, David
  last_name: De Gorter
- first_name: Florian
  full_name: Schur, Florian
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
- first_name: Jonathan
  full_name: Bayerl, Jonathan
  last_name: Bayerl
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Stefan
  full_name: Wieser, Stefan
  id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
  last_name: Wieser
  orcid: 0000-0002-2670-2217
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Frank
  full_name: Lai, Frank
  last_name: Lai
- first_name: Markus
  full_name: Moser, Markus
  last_name: Moser
- first_name: Dontscho
  full_name: Kerjaschki, Dontscho
  last_name: Kerjaschki
- first_name: Klemens
  full_name: Rottner, Klemens
  last_name: Rottner
- first_name: Victor
  full_name: Small, Victor
  last_name: Small
- first_name: Theresia
  full_name: Stradal, Theresia
  last_name: Stradal
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Leithner AF, Eichner A, Müller J, et al. Diversified actin protrusions promote
    environmental exploration but are dispensable for locomotion of leukocytes. <i>Nature
    Cell Biology</i>. 2016;18:1253-1259. doi:<a href="https://doi.org/10.1038/ncb3426">10.1038/ncb3426</a>
  apa: Leithner, A. F., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz,
    J., … Sixt, M. K. (2016). Diversified actin protrusions promote environmental
    exploration but are dispensable for locomotion of leukocytes. <i>Nature Cell Biology</i>.
    Nature Publishing Group. <a href="https://doi.org/10.1038/ncb3426">https://doi.org/10.1038/ncb3426</a>
  chicago: Leithner, Alexander F, Alexander Eichner, Jan Müller, Anne Reversat, Markus
    Brown, Jan Schwarz, Jack Merrin, et al. “Diversified Actin Protrusions Promote
    Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” <i>Nature
    Cell Biology</i>. Nature Publishing Group, 2016. <a href="https://doi.org/10.1038/ncb3426">https://doi.org/10.1038/ncb3426</a>.
  ieee: A. F. Leithner <i>et al.</i>, “Diversified actin protrusions promote environmental
    exploration but are dispensable for locomotion of leukocytes,” <i>Nature Cell
    Biology</i>, vol. 18. Nature Publishing Group, pp. 1253–1259, 2016.
  ista: Leithner AF, Eichner A, Müller J, Reversat A, Brown M, Schwarz J, Merrin J,
    De Gorter D, Schur FK, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai F, Moser
    M, Kerjaschki D, Rottner K, Small V, Stradal T, Sixt MK. 2016. Diversified actin
    protrusions promote environmental exploration but are dispensable for locomotion
    of leukocytes. Nature Cell Biology. 18, 1253–1259.
  mla: Leithner, Alexander F., et al. “Diversified Actin Protrusions Promote Environmental
    Exploration but Are Dispensable for Locomotion of Leukocytes.” <i>Nature Cell
    Biology</i>, vol. 18, Nature Publishing Group, 2016, pp. 1253–59, doi:<a href="https://doi.org/10.1038/ncb3426">10.1038/ncb3426</a>.
  short: A.F. Leithner, A. Eichner, J. Müller, A. Reversat, M. Brown, J. Schwarz,
    J. Merrin, D. De Gorter, F.K. Schur, J. Bayerl, I. de Vries, S. Wieser, R. Hauschild,
    F. Lai, M. Moser, D. Kerjaschki, K. Rottner, V. Small, T. Stradal, M.K. Sixt,
    Nature Cell Biology 18 (2016) 1253–1259.
date_created: 2018-12-11T11:51:21Z
date_published: 2016-10-24T00:00:00Z
date_updated: 2024-03-25T23:30:09Z
day: '24'
ddc:
- '570'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/ncb3426
ec_funded: 1
file:
- access_level: open_access
  checksum: e1411cb7c99a2d9089c178a6abef25e7
  content_type: application/pdf
  creator: dernst
  date_created: 2020-05-14T16:33:46Z
  date_updated: 2020-07-14T12:44:43Z
  file_id: '7844'
  file_name: 2018_NatureCell_Leithner.pdf
  file_size: 4433280
  relation: main_file
file_date_updated: 2020-07-14T12:44:43Z
has_accepted_license: '1'
intvolume: '        18'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 1253 - 1259
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5949'
quality_controlled: '1'
related_material:
  record:
  - id: '323'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Diversified actin protrusions promote environmental exploration but are dispensable
  for locomotion of leukocytes
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
    BY-NC-SA 4.0)
  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2016'
...
---
_id: '1561'
abstract:
- lang: eng
  text: Replication-deficient recombinant adenoviruses are potent vectors for the
    efficient transient expression of exogenous genes in resting immune cells. However,
    most leukocytes are refractory to efficient adenoviral transduction as they lack
    expression of the coxsackie/adenovirus receptor (CAR). To circumvent this obstacle,
    we generated the R26/CAG-CARΔ1StopF (where R26 is ROSA26 and CAG is CMV early
    enhancer/chicken β actin promoter) knock-in mouse line. This strain allows monitoring
    of in situ Cre recombinase activity through expression of CARΔ1. Simultaneously,
    CARΔ1 expression permits selective and highly efficient adenoviral transduction
    of immune cell populations, such as mast cells or T cells, directly ex vivo in
    bulk cultures without prior cell purification or activation. Furthermore, we show
    that CARΔ1 expression dramatically improves adenoviral infection of in vitro differentiated
    conventional and plasmacytoid dendritic cells (DCs), basophils, mast cells, as
    well as Hoxb8-immortalized hematopoietic progenitor cells. This novel dual function
    mouse strain will hence be a valuable tool to rapidly dissect the function of
    specific genes in leukocyte physiology.
author:
- first_name: Klaus
  full_name: Heger, Klaus
  last_name: Heger
- first_name: Maike
  full_name: Kober, Maike
  last_name: Kober
- first_name: David
  full_name: Rieß, David
  last_name: Rieß
- first_name: Christoph
  full_name: Drees, Christoph
  last_name: Drees
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Arianna
  full_name: Bertossi, Arianna
  last_name: Bertossi
- first_name: Axel
  full_name: Roers, Axel
  last_name: Roers
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Marc
  full_name: Schmidt Supprian, Marc
  last_name: Schmidt Supprian
citation:
  ama: Heger K, Kober M, Rieß D, et al. A novel Cre recombinase reporter mouse strain
    facilitates selective and efficient infection of primary immune cells with adenoviral
    vectors. <i>European Journal of Immunology</i>. 2015;45(6):1614-1620. doi:<a href="https://doi.org/10.1002/eji.201545457">10.1002/eji.201545457</a>
  apa: Heger, K., Kober, M., Rieß, D., Drees, C., de Vries, I., Bertossi, A., … Schmidt
    Supprian, M. (2015). A novel Cre recombinase reporter mouse strain facilitates
    selective and efficient infection of primary immune cells with adenoviral vectors.
    <i>European Journal of Immunology</i>. Wiley. <a href="https://doi.org/10.1002/eji.201545457">https://doi.org/10.1002/eji.201545457</a>
  chicago: Heger, Klaus, Maike Kober, David Rieß, Christoph Drees, Ingrid de Vries,
    Arianna Bertossi, Axel Roers, Michael K Sixt, and Marc Schmidt Supprian. “A Novel
    Cre Recombinase Reporter Mouse Strain Facilitates Selective and Efficient Infection
    of Primary Immune Cells with Adenoviral Vectors.” <i>European Journal of Immunology</i>.
    Wiley, 2015. <a href="https://doi.org/10.1002/eji.201545457">https://doi.org/10.1002/eji.201545457</a>.
  ieee: K. Heger <i>et al.</i>, “A novel Cre recombinase reporter mouse strain facilitates
    selective and efficient infection of primary immune cells with adenoviral vectors,”
    <i>European Journal of Immunology</i>, vol. 45, no. 6. Wiley, pp. 1614–1620, 2015.
  ista: Heger K, Kober M, Rieß D, Drees C, de Vries I, Bertossi A, Roers A, Sixt MK,
    Schmidt Supprian M. 2015. A novel Cre recombinase reporter mouse strain facilitates
    selective and efficient infection of primary immune cells with adenoviral vectors.
    European Journal of Immunology. 45(6), 1614–1620.
  mla: Heger, Klaus, et al. “A Novel Cre Recombinase Reporter Mouse Strain Facilitates
    Selective and Efficient Infection of Primary Immune Cells with Adenoviral Vectors.”
    <i>European Journal of Immunology</i>, vol. 45, no. 6, Wiley, 2015, pp. 1614–20,
    doi:<a href="https://doi.org/10.1002/eji.201545457">10.1002/eji.201545457</a>.
  short: K. Heger, M. Kober, D. Rieß, C. Drees, I. de Vries, A. Bertossi, A. Roers,
    M.K. Sixt, M. Schmidt Supprian, European Journal of Immunology 45 (2015) 1614–1620.
date_created: 2018-12-11T11:52:44Z
date_published: 2015-06-01T00:00:00Z
date_updated: 2021-01-12T06:51:36Z
day: '01'
department:
- _id: MiSi
doi: 10.1002/eji.201545457
intvolume: '        45'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 1614 - 1620
publication: European Journal of Immunology
publication_status: published
publisher: Wiley
publist_id: '5610'
quality_controlled: '1'
scopus_import: 1
status: public
title: A novel Cre recombinase reporter mouse strain facilitates selective and efficient
  infection of primary immune cells with adenoviral vectors
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2015'
...
---
_id: '2839'
abstract:
- lang: eng
  text: Directional guidance of cells via gradients of chemokines is considered crucial
    for embryonic development, cancer dissemination, and immune responses. Nevertheless,
    the concept still lacks direct experimental confirmation in vivo. Here, we identify
    endogenous gradients of the chemokine CCL21 within mouse skin and show that they
    guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots
    of CCL21 within lymphatic endothelial cells and steeply decaying gradients within
    the perilymphatic interstitium. These gradients match the migratory patterns of
    the dendritic cells, which directionally approach vessels from a distance of up
    to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and
    its experimental delocalization or swamping the endogenous gradients abolishes
    directed migration. These findings functionally establish the concept of haptotaxis,
    directed migration along immobilized gradients, in tissues.
acknowledgement: We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst,
  and E. Kiermaier for critical reading of the manuscript. This work was supported
  by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research
  Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011
  to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss
  National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).
article_processing_charge: No
article_type: original
author:
- first_name: Michele
  full_name: Weber, Michele
  id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
  last_name: Weber
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Christine
  full_name: Moussion, Christine
  id: 3356F664-F248-11E8-B48F-1D18A9856A87
  last_name: Moussion
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Daniel
  full_name: Legler, Daniel
  last_name: Legler
- first_name: Sanjiv
  full_name: Luther, Sanjiv
  last_name: Luther
- first_name: Mark Tobias
  full_name: Bollenbach, Mark Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance
    by haptotactic chemokine gradients. <i>Science</i>. 2013;339(6117):328-332. doi:<a
    href="https://doi.org/10.1126/science.1228456">10.1126/science.1228456</a>
  apa: Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler,
    D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic
    chemokine gradients. <i>Science</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/science.1228456">https://doi.org/10.1126/science.1228456</a>
  chicago: Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid
    de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K
    Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.”
    <i>Science</i>. American Association for the Advancement of Science, 2013. <a
    href="https://doi.org/10.1126/science.1228456">https://doi.org/10.1126/science.1228456</a>.
  ieee: M. Weber <i>et al.</i>, “Interstitial dendritic cell guidance by haptotactic
    chemokine gradients,” <i>Science</i>, vol. 339, no. 6117. American Association
    for the Advancement of Science, pp. 328–332, 2013.
  ista: Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther
    S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic
    chemokine gradients. Science. 339(6117), 328–332.
  mla: Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic
    Chemokine Gradients.” <i>Science</i>, vol. 339, no. 6117, American Association
    for the Advancement of Science, 2013, pp. 328–32, doi:<a href="https://doi.org/10.1126/science.1228456">10.1126/science.1228456</a>.
  short: M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler,
    S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.
date_created: 2018-12-11T11:59:52Z
date_published: 2013-01-18T00:00:00Z
date_updated: 2022-06-10T10:21:40Z
day: '18'
department:
- _id: MiSi
- _id: Bio
doi: 10.1126/science.1228456
ec_funded: 1
intvolume: '       339'
issue: '6117'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf
month: '01'
oa: 1
oa_version: Published Version
page: 328 - 332
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
- _id: 25ABD200-B435-11E9-9278-68D0E5697425
  grant_number: RGP0058/2011
  name: 'Cell migration in complex environments: from in vivo experiments to theoretical
    models'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3959'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Interstitial dendritic cell guidance by haptotactic chemokine gradients
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 339
year: '2013'
...