---
_id: '14846'
abstract:
- lang: eng
  text: Contraction and flow of the actin cell cortex have emerged as a common principle
    by which cells reorganize their cytoplasm and take shape. However, how these cortical
    flows interact with adjacent cytoplasmic components, changing their form and localization,
    and how this affects cytoplasmic organization and cell shape remains unclear.
    Here we show that in ascidian oocytes, the cooperative activities of cortical
    actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive
    oocyte cytoplasmic reorganization and shape changes following fertilization. We
    show that vegetal-directed cortical actomyosin flows, established upon oocyte
    fertilization, lead to both the accumulation of cortical actin at the vegetal
    pole of the zygote and compression and local buckling of the adjacent elastic
    solid-like myoplasm layer due to friction forces generated at their interface.
    Once cortical flows have ceased, the multiple myoplasm buckles resolve into one
    larger buckle, which again drives the formation of the contraction pole—a protuberance
    of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings
    reveal a mechanism where cortical actomyosin network flows determine cytoplasmic
    reorganization and cell shape by deforming adjacent cytoplasmic components through
    friction forces.
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: NanoFab
acknowledgement: We would like to thank A. McDougall, E. Hannezo and the Heisenberg
  lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP
  and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific
  Service Units of the Institute of Science and Technology Austria through resources
  provided by the Electron Microscopy Facility, Imaging and Optics Facility and the
  Nanofabrication Facility. This work was supported by a Joint Project Grant from
  the FWF (I 3601-B27).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Silvia
  full_name: Caballero Mancebo, Silvia
  id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
  last_name: Caballero Mancebo
  orcid: 0000-0002-5223-3346
- first_name: Rushikesh
  full_name: Shinde, Rushikesh
  last_name: Shinde
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Matilda
  full_name: Peruzzo, Matilda
  id: 3F920B30-F248-11E8-B48F-1D18A9856A87
  last_name: Peruzzo
  orcid: 0000-0002-3415-4628
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Irene
  full_name: Steccari, Irene
  id: 2705C766-9FE2-11EA-B224-C6773DDC885E
  last_name: Steccari
- first_name: David
  full_name: Labrousse Arias, David
  id: CD573DF4-9ED3-11E9-9D77-3223E6697425
  last_name: Labrousse Arias
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Andrew
  full_name: Callan-Jones, Andrew
  last_name: Callan-Jones
- first_name: Raphaël
  full_name: Voituriez, Raphaël
  last_name: Voituriez
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine
    cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.
    <i>Nature Physics</i>. 2024. doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>
  apa: Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G.,
    Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature
    Physics</i>. Springer Nature. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>
  chicago: Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda
    Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction
    Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes
    upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>.
  ieee: S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature
    Physics</i>. Springer Nature, 2024.
  ista: Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari
    I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg
    C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes
    of ascidian oocytes upon fertilization. Nature Physics.
  mla: Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization
    and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>,
    Springer Nature, 2024, doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>.
  short: S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I.
    Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez,
    C.-P.J. Heisenberg, Nature Physics (2024).
date_created: 2024-01-21T23:00:57Z
date_published: 2024-01-09T00:00:00Z
date_updated: 2024-03-05T09:33:38Z
day: '09'
department:
- _id: CaHe
- _id: JoFi
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
doi: 10.1038/s41567-023-02302-1
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1038/s41567-023-02302-1
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03601
  name: Control of embryonic cleavage pattern
publication: Nature Physics
publication_identifier:
  eissn:
  - 1745-2481
  issn:
  - 1745-2473
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/
scopus_import: '1'
status: public
title: Friction forces determine cytoplasmic reorganization and shape changes of ascidian
  oocytes upon fertilization
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '727'
abstract:
- lang: eng
  text: 'Actin filaments polymerizing against membranes power endocytosis, vesicular
    traffic, and cell motility. In vitro reconstitution studies suggest that the structure
    and the dynamics of actin networks respond to mechanical forces. We demonstrate
    that lamellipodial actin of migrating cells responds to mechanical load when membrane
    tension is modulated. In a steady state, migrating cell filaments assume the canonical
    dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension
    triggers a dense network with a broadened range of angles, whereas decreased tension
    causes a shift to a sparse configuration dominated by filaments growing perpendicularly
    to the plasma membrane. We show that these responses emerge from the geometry
    of branched actin: when load per filament decreases, elongation speed increases
    and perpendicular filaments gradually outcompete others because they polymerize
    the shortest distance to the membrane, where they are protected from capping.
    This network-intrinsic geometrical adaptation mechanism tunes protrusive force
    in response to mechanical load.'
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: Jan
  full_name: Mueller, Jan
  last_name: Mueller
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Arnon
  full_name: Lieber, Arnon
  last_name: Lieber
- first_name: Christoph
  full_name: Winkler, Christoph
  last_name: Winkler
- first_name: Karsten
  full_name: Kruse, Karsten
  last_name: Kruse
- first_name: John
  full_name: Small, John
  last_name: Small
- first_name: Christian
  full_name: Schmeiser, Christian
  last_name: Schmeiser
- first_name: Kinneret
  full_name: Keren, Kinneret
  last_name: Keren
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin
    networks. <i>Cell</i>. 2017;171(1):188-200. doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>
  apa: Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C.,
    … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. <i>Cell</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>
  chicago: Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber,
    Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin
    Networks.” <i>Cell</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>.
  ieee: J. Mueller <i>et al.</i>, “Load adaptation of lamellipodial actin networks,”
    <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.
  ista: Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K,
    Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of
    lamellipodial actin networks. Cell. 171(1), 188–200.
  mla: Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>,
    vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>.
  short: J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K.
    Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017)
    188–200.
date_created: 2018-12-11T11:48:10Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2023-09-28T11:33:49Z
day: '21'
department:
- _id: MiSi
- _id: Bio
doi: 10.1016/j.cell.2017.07.051
ec_funded: 1
external_id:
  isi:
  - '000411331800020'
intvolume: '       171'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
page: 188 - 200
project:
- _id: 25AD6156-B435-11E9-9278-68D0E5697425
  grant_number: LS13-029
  name: Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
    (EU)
publication: Cell
publication_identifier:
  issn:
  - '00928674'
publication_status: published
publisher: Cell Press
publist_id: '6951'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Load adaptation of lamellipodial actin networks
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 171
year: '2017'
...