---
_id: '147'
abstract:
- lang: eng
  text: The trafficking of subcellular cargos in eukaryotic cells crucially depends
    on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine
    nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis,
    vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they
    are important for plant development, mainly through controlling the polar subcellular
    localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here,
    using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin
    4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically
    with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular
    trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally,
    Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show
    altered sensitivity to ES4. ES4 interferes with the activation-based membrane
    association of the ARF1 GTPases, but not of their mutant variants that are activated
    independently of ARF-GEF activity. Biochemical approaches and docking simulations
    confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These
    observations collectively identify ES4 as a chemical tool enabling the study of
    ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.
acknowledgement: We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing
  antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert,
  Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa
  Doyle for critical reading of the manuscript and helpful comments and suggestions;
  and Stephanie Smith and Martine De Cock for help in editing and language corrections.
  We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging
  large RI project (LM2015062 funded by MEYS CR) for their support with obtaining
  scientific data presented in this article. Plant Sciences Core Facility of CEITEC
  Masaryk University is gratefully acknowledged for obtaining part of the scientific
  data presented in this article. We acknowledge support from the Fondation pour la
  Recherche Médicale and from the Institut National du Cancer (J.C.). The research
  leading to these results was funded by the European Research Council under the European
  Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300
  and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of
  Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC
  2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.);
  a joint research project within the framework of cooperation between the Research
  Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and
  E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och
  Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna
  (P.G.), Tryggers CTS410 (P.G.).
article_processing_charge: No
article_type: original
author:
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Tomasz
  full_name: Nodzyński, Tomasz
  last_name: Nodzyński
- first_name: Qing
  full_name: Lu, Qing
  last_name: Lu
- first_name: Glenn R
  full_name: Hicks, Glenn R
  last_name: Hicks
- first_name: Wim
  full_name: Nerinckx, Wim
  last_name: Nerinckx
- first_name: Kiril
  full_name: Mishev, Kiril
  last_name: Mishev
- first_name: Francois
  full_name: Peurois, Francois
  last_name: Peurois
- first_name: Jacqueline
  full_name: Cherfils, Jacqueline
  last_name: Cherfils
- first_name: Rycke Riet Maria
  full_name: De, Rycke Riet Maria
  last_name: De
- first_name: Peter
  full_name: Grones, Peter
  id: 399876EC-F248-11E8-B48F-1D18A9856A87
  last_name: Grones
- first_name: Stéphanie
  full_name: Robert, Stéphanie
  last_name: Robert
- first_name: Eugenia
  full_name: Russinova, Eugenia
  last_name: Russinova
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Kania U, Nodzyński T, Lu Q, et al. The inhibitor Endosidin 4 targets SEC7 domain-type
    ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes.
    <i>The Plant Cell</i>. 2018;30(10):2553-2572. doi:<a href="https://doi.org/10.1105/tpc.18.00127">10.1105/tpc.18.00127</a>
  apa: Kania, U., Nodzyński, T., Lu, Q., Hicks, G. R., Nerinckx, W., Mishev, K., …
    Friml, J. (2018). The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase
    exchange factors and interferes with sub cellular trafficking in eukaryotes. <i>The
    Plant Cell</i>. Oxford University Press. <a href="https://doi.org/10.1105/tpc.18.00127">https://doi.org/10.1105/tpc.18.00127</a>
  chicago: Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx,
    Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7
    Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking
    in Eukaryotes.” <i>The Plant Cell</i>. Oxford University Press, 2018. <a href="https://doi.org/10.1105/tpc.18.00127">https://doi.org/10.1105/tpc.18.00127</a>.
  ieee: U. Kania <i>et al.</i>, “The inhibitor Endosidin 4 targets SEC7 domain-type
    ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes,”
    <i>The Plant Cell</i>, vol. 30, no. 10. Oxford University Press, pp. 2553–2572,
    2018.
  ista: Kania U, Nodzyński T, Lu Q, Hicks GR, Nerinckx W, Mishev K, Peurois F, Cherfils
    J, De RRM, Grones P, Robert S, Russinova E, Friml J. 2018. The inhibitor Endosidin
    4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub
    cellular trafficking in eukaryotes. The Plant Cell. 30(10), 2553–2572.
  mla: Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type
    ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.”
    <i>The Plant Cell</i>, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72,
    doi:<a href="https://doi.org/10.1105/tpc.18.00127">10.1105/tpc.18.00127</a>.
  short: U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois,
    J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant
    Cell 30 (2018) 2553–2572.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2025-05-07T11:12:30Z
day: '12'
department:
- _id: JiFr
doi: 10.1105/tpc.18.00127
ec_funded: 1
external_id:
  isi:
  - '000450000500023'
  pmid:
  - '30018156'
intvolume: '        30'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1105/tpc.18.00127
month: '11'
oa: 1
oa_version: Published Version
page: 2553 - 2572
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: The Plant Cell
publication_identifier:
  issn:
  - 1040-4651
publication_status: published
publisher: Oxford University Press
publist_id: '7776'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors
  and interferes with sub cellular trafficking in eukaryotes
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '412'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
    cargoes and lipids are internalized from the plasma membrane into vesicles coated
    with clathrin and adaptor proteins. CME is essential for many developmental and
    physiological processes in plants, but its underlying mechanism is not well characterised
    compared to that in yeast and animal systems. Here, we searched for new factors
    involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
    of proteins that interact with clathrin light chain, a principal component of
    the clathrin coat. Among the confirmed interactors, we found two putative homologues
    of the clathrin-coat uncoating factor auxilin previously described in non-plant
    systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
    an arrest of seedling growth and development. This was concomitant with inhibited
    endocytosis due to blocking of clathrin recruitment after the initial step of
    adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
    loss-of-function lines did not present endocytosis-related developmental or cellular
    phenotypes under normal growth conditions. This work contributes to the on-going
    characterization of the endocytotic machinery in plants and provides a robust
    tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
  at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
  construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
  Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
  Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
  help with correcting the manuscript. This work was supported by the European Research
  Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
  Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
  NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Geert
  full_name: De Jaeger, Geert
  last_name: De Jaeger
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
    study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
    <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>
  apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml,
    J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
    factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>
  chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
    Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
    Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American
    Society of Plant Biologists, 2018. <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>.
  ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
    “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant
    Biologists, pp. 700–716, 2018.
  ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
    functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis. The Plant Cell. 30(3), 700–716.
  mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
    Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no.
    3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>.
  short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
    The Plant Cell 30 (2018) 700–716.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2025-05-07T11:12:27Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
  isi:
  - '000429441400018'
  pmid:
  - '29511054'
file:
- access_level: open_access
  checksum: 4e165e653b67d3f0684697f21aace5a1
  content_type: application/pdf
  creator: dernst
  date_created: 2022-05-23T09:12:38Z
  date_updated: 2022-05-23T09:12:38Z
  file_id: '11406'
  file_name: 2018_PlantCell_Adamowski.pdf
  file_size: 4407538
  relation: main_file
  success: 1
file_date_updated: 2022-05-23T09:12:38Z
has_accepted_license: '1'
intvolume: '        30'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
related_material:
  record:
  - id: '6269'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
  in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '2188'
abstract:
- lang: eng
  text: Although plant and animal cells use a similar core mechanism to deliver proteins
    to the plasma membrane, their different lifestyle, body organization and specific
    cell structures resulted in the acquisition of regulatory mechanisms that vary
    in the two kingdoms. In particular, cell polarity regulators do not seem to be
    conserved, because genes encoding key components are absent in plant genomes.
    In plants, the broad knowledge on polarity derives from the study of auxin transporters,
    the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals,
    much information is provided from the study of polarity in epithelial cells that
    exhibit basolateral and luminal apical polarities, separated by tight junctions.
    In this review, we summarize the similarities and differences of the polarization
    mechanisms between plants and animals and survey the main genetic approaches that
    have been used to characterize new genes involved in polarity establishment in
    plants, including the frequently used forward and reverse genetics screens as
    well as a novel chemical genetics approach that is expected to overcome the limitation
    of classical genetics methods.
acknowledgement: "This work was supported by a grant from the Research Foundation-Flanders
  (Odysseus).\r\n\r\n"
article_number: '140017'
author:
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Matyas
  full_name: Fendrych, Matyas
  last_name: Fendrych
- first_name: Jiřĺ
  full_name: Friml, Jiřĺ
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences
    to animal epithelial cells. <i>Open Biology</i>. 2014;4(APRIL). doi:<a href="https://doi.org/10.1098/rsob.140017">10.1098/rsob.140017</a>
  apa: Kania, U., Fendrych, M., &#38; Friml, J. (2014). Polar delivery in plants;
    commonalities and differences to animal epithelial cells. <i>Open Biology</i>.
    Royal Society. <a href="https://doi.org/10.1098/rsob.140017">https://doi.org/10.1098/rsob.140017</a>
  chicago: Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants;
    Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>.
    Royal Society, 2014. <a href="https://doi.org/10.1098/rsob.140017">https://doi.org/10.1098/rsob.140017</a>.
  ieee: U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities
    and differences to animal epithelial cells,” <i>Open Biology</i>, vol. 4, no.
    APRIL. Royal Society, 2014.
  ista: Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities
    and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.
  mla: Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences
    to Animal Epithelial Cells.” <i>Open Biology</i>, vol. 4, no. APRIL, 140017, Royal
    Society, 2014, doi:<a href="https://doi.org/10.1098/rsob.140017">10.1098/rsob.140017</a>.
  short: U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014).
date_created: 2018-12-11T11:56:13Z
date_published: 2014-04-16T00:00:00Z
date_updated: 2021-01-12T06:55:52Z
day: '16'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1098/rsob.140017
file:
- access_level: open_access
  checksum: 2020627feff36cf0799167c84149fa75
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:40Z
  date_updated: 2020-07-14T12:45:31Z
  file_id: '5025'
  file_name: IST-2016-441-v1+1_140017.full.pdf
  file_size: 682570
  relation: main_file
file_date_updated: 2020-07-14T12:45:31Z
has_accepted_license: '1'
intvolume: '         4'
issue: APRIL
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
publication: Open Biology
publication_status: published
publisher: Royal Society
publist_id: '4786'
pubrep_id: '441'
quality_controlled: '1'
scopus_import: 1
status: public
title: Polar delivery in plants; commonalities and differences to animal epithelial
  cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2014'
...
---
_id: '2240'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma
    membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is
    essential for physiology and development, but the identification and organization
    of the machinery operating this process remains largely obscure. Here, we identified
    an eight-core-component protein complex, the TPLATE complex, essential for plant
    growth via its role as major adaptor module for clathrin-mediated endocytosis.
    This complex consists of evolutionarily unique proteins that associate closely
    with core endocytic elements. The TPLATE complex is recruited as dynamic foci
    at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin,
    and dynamin-related proteins. Reduced function of different complex components
    severely impaired internalization of assorted endocytic cargoes, demonstrating
    its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE
    complex is an early endocytic module representing a unique evolutionary plant
    adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
author:
- first_name: Astrid
  full_name: Gadeyne, Astrid
  last_name: Gadeyne
- first_name: Clara
  full_name: Sánchez Rodríguez, Clara
  last_name: Sánchez Rodríguez
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
- first_name: Simone
  full_name: Di Rubbo, Simone
  last_name: Di Rubbo
- first_name: Henrik
  full_name: Zauber, Henrik
  last_name: Zauber
- first_name: Kevin
  full_name: Vanneste, Kevin
  last_name: Vanneste
- first_name: Jelle
  full_name: Van Leene, Jelle
  last_name: Van Leene
- first_name: Nancy
  full_name: De Winne, Nancy
  last_name: De Winne
- first_name: Dominique
  full_name: Eeckhout, Dominique
  last_name: Eeckhout
- first_name: Geert
  full_name: Persiau, Geert
  last_name: Persiau
- first_name: Eveline
  full_name: Van De Slijke, Eveline
  last_name: Van De Slijke
- first_name: Bernard
  full_name: Cannoot, Bernard
  last_name: Cannoot
- first_name: Leen
  full_name: Vercruysse, Leen
  last_name: Vercruysse
- first_name: Jonathan
  full_name: Mayers, Jonathan
  last_name: Mayers
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Matthias
  full_name: Ehrlich, Matthias
  last_name: Ehrlich
- first_name: Alois
  full_name: Schweighofer, Alois
  last_name: Schweighofer
- first_name: Tijs
  full_name: Ketelaar, Tijs
  last_name: Ketelaar
- first_name: Steven
  full_name: Maere, Steven
  last_name: Maere
- first_name: Sebastian
  full_name: Bednarek, Sebastian
  last_name: Bednarek
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Kris
  full_name: Gevaert, Kris
  last_name: Gevaert
- first_name: Erwin
  full_name: Witters, Erwin
  last_name: Witters
- first_name: Eugenia
  full_name: Russinova, Eugenia
  last_name: Russinova
- first_name: Staffan
  full_name: Persson, Staffan
  last_name: Persson
- first_name: Geert
  full_name: De Jaeger, Geert
  last_name: De Jaeger
- first_name: Daniël
  full_name: Van Damme, Daniël
  last_name: Van Damme
citation:
  ama: Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex
    drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704.
    doi:<a href="https://doi.org/10.1016/j.cell.2014.01.039">10.1016/j.cell.2014.01.039</a>
  apa: Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H.,
    Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated
    endocytosis in plants. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2014.01.039">https://doi.org/10.1016/j.cell.2014.01.039</a>
  chicago: Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo,
    Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex
    Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014.
    <a href="https://doi.org/10.1016/j.cell.2014.01.039">https://doi.org/10.1016/j.cell.2014.01.039</a>.
  ieee: A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated
    endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704,
    2014.
  ista: Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste
    K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B,
    Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar
    T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S,
    De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated
    endocytosis in plants. Cell. 156(4), 691–704.
  mla: Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated
    Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704,
    doi:<a href="https://doi.org/10.1016/j.cell.2014.01.039">10.1016/j.cell.2014.01.039</a>.
  short: A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K.
    Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke,
    B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer,
    T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova,
    S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.
date_created: 2018-12-11T11:56:31Z
date_published: 2014-02-13T00:00:00Z
date_updated: 2021-01-12T06:56:13Z
day: '13'
department:
- _id: JiFr
doi: 10.1016/j.cell.2014.01.039
intvolume: '       156'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 691 - 704
publication: Cell
publication_identifier:
  issn:
  - '00928674'
publication_status: published
publisher: Cell Press
publist_id: '4721'
quality_controlled: '1'
scopus_import: 1
status: public
title: The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 156
year: '2014'
...
---
_id: '2919'
abstract:
- lang: eng
  text: The distribution of the phytohormone auxin regulates many aspects of plant
    development including growth response to gravity. Gravitropic root curvature involves
    coordinated and asymmetric cell elongation between the lower and upper side of
    the root, mediated by differential cellular auxin levels. The asymmetry in the
    auxin distribution is established and maintained by a spatio-temporal regulation
    of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights
    into the complex regulation of PIN abundance and activity during root gravitropism.
    We show that PIN2 turnover is differentially regulated on the upper and lower
    side of gravistimulated roots by distinct but partially overlapping auxin feedback
    mechanisms. In addition to regulating transcription and clathrin-mediated internalization,
    auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar
    targeting and degradation. This effect of elevated auxin levels requires the activity
    of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also
    suboptimal auxin levels mediate PIN degradation utilizing the same signalling
    pathway. These feedback mechanisms are functionally important during gravitropic
    response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating
    asymmetric growth.
author:
- first_name: Pawel
  full_name: Baster, Pawel
  id: 3028BD74-F248-11E8-B48F-1D18A9856A87
  last_name: Baster
- first_name: Stéphanie
  full_name: Robert, Stéphanie
  last_name: Robert
- first_name: Jürgen
  full_name: Kleine Vehn, Jürgen
  last_name: Kleine Vehn
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Wim
  full_name: Grunewald, Wim
  last_name: Grunewald
- first_name: Bert
  full_name: De Rybel, Bert
  last_name: De Rybel
- first_name: Tom
  full_name: Beeckman, Tom
  last_name: Beeckman
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates
    PIN vacuolar trafficking and auxin fluxes during root gravitropism. <i>EMBO Journal</i>.
    2013;32(2):260-274. doi:<a href="https://doi.org/10.1038/emboj.2012.310">10.1038/emboj.2012.310</a>
  apa: Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald,
    W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking
    and auxin fluxes during root gravitropism. <i>EMBO Journal</i>. Wiley-Blackwell.
    <a href="https://doi.org/10.1038/emboj.2012.310">https://doi.org/10.1038/emboj.2012.310</a>
  chicago: Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste,
    Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1
    AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during
    Root Gravitropism.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href="https://doi.org/10.1038/emboj.2012.310">https://doi.org/10.1038/emboj.2012.310</a>.
  ieee: P. Baster <i>et al.</i>, “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
    trafficking and auxin fluxes during root gravitropism,” <i>EMBO Journal</i>, vol.
    32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.
  ista: Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel
    B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
    trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274.
  mla: Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar
    Trafficking and Auxin Fluxes during Root Gravitropism.” <i>EMBO Journal</i>, vol.
    32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:<a href="https://doi.org/10.1038/emboj.2012.310">10.1038/emboj.2012.310</a>.
  short: P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald,
    B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.
date_created: 2018-12-11T12:00:20Z
date_published: 2013-01-23T00:00:00Z
date_updated: 2021-01-12T07:00:41Z
day: '23'
department:
- _id: JiFr
doi: 10.1038/emboj.2012.310
external_id:
  pmid:
  - '23211744'
intvolume: '        32'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/
month: '01'
oa: 1
oa_version: Submitted Version
page: 260 - 274
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3818'
quality_controlled: '1'
scopus_import: 1
status: public
title: SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin
  fluxes during root gravitropism
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2013'
...
---
_id: '3110'
abstract:
- lang: eng
  text: 'The directional transport of the phytohormone auxin depends on the phosphorylation
    status and polar localization of PIN-FORMED (PIN) auxin efflux proteins. While
    PINIOD (PID) kinase is directly involved in the phosphorylation of PIN proteins,
    the phosphatase holoenzyme complexes that dephosphorylate PIN proteins remain
    elusive. Here, we demonstrate that mutations simultaneously disrupting the function
    of Arabidopsis thaliana FyPP1 (for Phytochrome-associated serine/threonine protein
    phosphatase1) and FyPP3, two homologous genes encoding the catalytic subunits
    of protein phosphatase6 (PP6), cause elevated accumulation of phosphorylated PIN
    proteins, correlating with a basal-to-apical shift in subcellular PIN localization.
    The changes in PIN polarity result in increased root basipetal auxin transport
    and severe defects, including shorter roots, fewer lateral roots, defective columella
    cells, root meristem collapse, abnormal cotyledons (small, cup-shaped, or fused
    cotyledons), and altered leaf venation. Our molecular, biochemical, and genetic
    data support the notion that FyPP1/3, SAL (for SAPS DOMAIN-LIKE), and PP2AA proteins
    (RCN1 [for ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1] or PP2AA1, PP2AA2, and PP2AA3)
    physically interact to form a novel PP6-type heterotrimeric holoenzyme complex.
    We also show that FyPP1/3, SAL, and PP2AA interact with a subset of PIN proteins
    and that for SAL the strength of the interaction depends on the PIN phosphorylation
    status. Thus, an Arabidopsis PP6-type phosphatase holoenzyme acts antagonistically
    with PID to direct auxin transport polarity and plant development by directly
    regulating PIN phosphorylation. '
author:
- first_name: Mingqiu
  full_name: Dai, Mingqiu
  last_name: Dai
- first_name: Chen
  full_name: Zhang, Chen
  last_name: Zhang
- first_name: Urszula
  full_name: Urszula Kania
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Fang
  full_name: Chen, Fang
  last_name: Chen
- first_name: Qin
  full_name: Xue, Qin
  last_name: Xue
- first_name: Tyra
  full_name: McCray, Tyra
  last_name: Mccray
- first_name: Gang
  full_name: Li, Gang
  last_name: Li
- first_name: Genji
  full_name: Qin, Genji
  last_name: Qin
- first_name: Michelle
  full_name: Wakeley, Michelle
  last_name: Wakeley
- first_name: William
  full_name: Terzaghi, William
  last_name: Terzaghi
- first_name: Jianmin
  full_name: Wan, Jianmin
  last_name: Wan
- first_name: Yunde
  full_name: Zhao, Yunde
  last_name: Zhao
- first_name: Jian
  full_name: Xu, Jian
  last_name: Xu
- first_name: Jirí
  full_name: Jirí Friml
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Xing
  full_name: Deng, Xing W
  last_name: Deng
- first_name: Haiyang
  full_name: Wang, Haiyang
  last_name: Wang
citation:
  ama: Dai M, Zhang C, Kania U, et al. A PP6 type phosphatase holoenzyme directly
    regulates PIN phosphorylation and auxin efflux in Arabidopsis. <i>Plant Cell</i>.
    2012;24(6):2497-2514. doi:<a href="https://doi.org/10.1105/tpc.112.098905">10.1105/tpc.112.098905</a>
  apa: Dai, M., Zhang, C., Kania, U., Chen, F., Xue, Q., Mccray, T., … Wang, H. (2012).
    A PP6 type phosphatase holoenzyme directly regulates PIN phosphorylation and auxin
    efflux in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.112.098905">https://doi.org/10.1105/tpc.112.098905</a>
  chicago: Dai, Mingqiu, Chen Zhang, Urszula Kania, Fang Chen, Qin Xue, Tyra Mccray,
    Gang Li, et al. “A PP6 Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation
    and Auxin Efflux in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant
    Biologists, 2012. <a href="https://doi.org/10.1105/tpc.112.098905">https://doi.org/10.1105/tpc.112.098905</a>.
  ieee: M. Dai <i>et al.</i>, “A PP6 type phosphatase holoenzyme directly regulates
    PIN phosphorylation and auxin efflux in Arabidopsis,” <i>Plant Cell</i>, vol.
    24, no. 6. American Society of Plant Biologists, pp. 2497–2514, 2012.
  ista: Dai M, Zhang C, Kania U, Chen F, Xue Q, Mccray T, Li G, Qin G, Wakeley M,
    Terzaghi W, Wan J, Zhao Y, Xu J, Friml J, Deng X, Wang H. 2012. A PP6 type phosphatase
    holoenzyme directly regulates PIN phosphorylation and auxin efflux in Arabidopsis.
    Plant Cell. 24(6), 2497–2514.
  mla: Dai, Mingqiu, et al. “A PP6 Type Phosphatase Holoenzyme Directly Regulates
    PIN Phosphorylation and Auxin Efflux in Arabidopsis.” <i>Plant Cell</i>, vol.
    24, no. 6, American Society of Plant Biologists, 2012, pp. 2497–514, doi:<a href="https://doi.org/10.1105/tpc.112.098905">10.1105/tpc.112.098905</a>.
  short: M. Dai, C. Zhang, U. Kania, F. Chen, Q. Xue, T. Mccray, G. Li, G. Qin, M.
    Wakeley, W. Terzaghi, J. Wan, Y. Zhao, J. Xu, J. Friml, X. Deng, H. Wang, Plant
    Cell 24 (2012) 2497–2514.
date_created: 2018-12-11T12:01:27Z
date_published: 2012-06-01T00:00:00Z
date_updated: 2021-01-12T07:41:08Z
day: '01'
doi: 10.1105/tpc.112.098905
extern: 1
intvolume: '        24'
issue: '6'
month: '06'
page: 2497 - 2514
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3589'
quality_controlled: 0
status: public
title: A PP6 type phosphatase holoenzyme directly regulates PIN phosphorylation and
  auxin efflux in Arabidopsis
type: journal_article
volume: 24
year: '2012'
...