[{"oa":1,"publication_identifier":{"issn":["0028646X"],"eissn":["14698137"]},"status":"public","ec_funded":1,"month":"01","year":"2021","date_updated":"2023-08-04T11:01:21Z","abstract":[{"text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.","lang":"eng"}],"issue":"1","_id":"8582","acknowledged_ssus":[{"_id":"Bio"}],"ddc":["580"],"file_date_updated":"2021-02-04T09:44:17Z","scopus_import":"1","citation":{"short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>","apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369.","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>."},"intvolume":"       229","publication_status":"published","doi":"10.1111/nph.16887","date_published":"2021-01-01T00:00:00Z","oa_version":"Published Version","title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","publisher":"Wiley","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"day":"01","acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","file":[{"file_size":4061962,"success":1,"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_id":"9084","creator":"dernst","checksum":"b45621607b4cab97eeb1605ab58e896e","date_created":"2021-02-04T09:44:17Z","file_name":"2021_NewPhytologist_Li.pdf","date_updated":"2021-02-04T09:44:17Z"}],"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"isi":1,"author":[{"full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"full_name":"von Wangenheim, Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247"},{"full_name":"Zhang, Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","last_name":"Zhang","first_name":"Xixi","orcid":"0000-0001-7048-4627"},{"last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285","first_name":"Shutang","full_name":"Tan, Shutang"},{"id":"39CD9926-F248-11E8-B48F-1D18A9856A87","last_name":"Darwish-Miranda","first_name":"Nasser","orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser"},{"full_name":"Naramoto, Satoshi","last_name":"Naramoto","first_name":"Satoshi"},{"orcid":"0000-0001-7263-0560","first_name":"Krzysztof T","last_name":"Wabnik","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","full_name":"Wabnik, Krzysztof T"},{"full_name":"de Rycke, Riet","first_name":"Riet","last_name":"de Rycke"},{"full_name":"Kaufmann, Walter","first_name":"Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann"},{"full_name":"Gütl, Daniel J","first_name":"Daniel J","id":"381929CE-F248-11E8-B48F-1D18A9856A87","last_name":"Gütl"},{"full_name":"Tejos, Ricardo","first_name":"Ricardo","last_name":"Tejos"},{"full_name":"Grones, Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","first_name":"Peter"},{"last_name":"Ke","first_name":"Meiyu","full_name":"Ke, Meiyu"},{"full_name":"Chen, Xu","first_name":"Xu","last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Dettmer, Jan","last_name":"Dettmer","first_name":"Jan"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"}],"article_processing_charge":"Yes (via OA deal)","article_type":"original","external_id":{"isi":["000570187900001"]},"volume":229,"quality_controlled":"1","page":"351-369","date_created":"2020-09-28T08:59:28Z","publication":"New Phytologist","has_accepted_license":"1","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"type":"journal_article"},{"publication_status":"published","doi":"10.1038/s41477-018-0212-z","date_published":"2018-07-30T00:00:00Z","oa_version":"Submitted Version","ddc":["580"],"scopus_import":"1","file_date_updated":"2020-07-14T12:44:56Z","citation":{"short":"C.L. Shi, D. von Wangenheim, U. Herrmann, M. Wildhagen, I. Kulik, A. Kopf, T. Ishida, V. Olsson, M.K. Anker, M. Albert, M.A. Butenko, G. Felix, S. Sawa, M. Claassen, J. Friml, R.B. Aalen, Nature Plants 4 (2018) 596–604.","ieee":"C. L. Shi <i>et al.</i>, “The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling,” <i>Nature Plants</i>, vol. 4, no. 8. Nature Publishing Group, pp. 596–604, 2018.","ama":"Shi CL, von Wangenheim D, Herrmann U, et al. The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. <i>Nature Plants</i>. 2018;4(8):596-604. doi:<a href=\"https://doi.org/10.1038/s41477-018-0212-z\">10.1038/s41477-018-0212-z</a>","ista":"Shi CL, von Wangenheim D, Herrmann U, Wildhagen M, Kulik I, Kopf A, Ishida T, Olsson V, Anker MK, Albert M, Butenko MA, Felix G, Sawa S, Claassen M, Friml J, Aalen RB. 2018. The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. 4(8), 596–604.","apa":"Shi, C. L., von Wangenheim, D., Herrmann, U., Wildhagen, M., Kulik, I., Kopf, A., … Aalen, R. B. (2018). The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. <i>Nature Plants</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41477-018-0212-z\">https://doi.org/10.1038/s41477-018-0212-z</a>","mla":"Shi, Chun Lin, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is Regulated by Peptide Signalling.” <i>Nature Plants</i>, vol. 4, no. 8, Nature Publishing Group, 2018, pp. 596–604, doi:<a href=\"https://doi.org/10.1038/s41477-018-0212-z\">10.1038/s41477-018-0212-z</a>.","chicago":"Shi, Chun Lin, Daniel von Wangenheim, Ullrich Herrmann, Mari Wildhagen, Ivan Kulik, Andreas Kopf, Takashi Ishida, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is Regulated by Peptide Signalling.” <i>Nature Plants</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41477-018-0212-z\">https://doi.org/10.1038/s41477-018-0212-z</a>."},"intvolume":"         4","year":"2018","date_updated":"2023-09-19T10:08:45Z","abstract":[{"lang":"eng","text":"The root cap protects the stem cell niche of angiosperm roots from damage. In Arabidopsis, lateral root cap (LRC) cells covering the meristematic zone are regularly lost through programmed cell death, while the outermost layer of the root cap covering the tip is repeatedly sloughed. Efficient coordination with stem cells producing new layers is needed to maintain a constant size of the cap. We present a signalling pair, the peptide IDA-LIKE1 (IDL1) and its receptor HAESA-LIKE2 (HSL2), mediating such communication. Live imaging over several days characterized this process from initial fractures in LRC cell files to full separation of a layer. Enhanced expression of IDL1 in the separating root cap layers resulted in increased frequency of sloughing, balanced with generation of new layers in a HSL2-dependent manner. Transcriptome analyses linked IDL1-HSL2 signalling to the transcription factors BEARSKIN1/2 and genes associated with programmed cell death. Mutations in either IDL1 or HSL2 slowed down cell division, maturation and separation. Thus, IDL1-HSL2 signalling potentiates dynamic regulation of the homeostatic balance between stem cell division and sloughing activity."}],"issue":"8","_id":"146","oa":1,"status":"public","month":"07","page":"596 - 604","date_created":"2018-12-11T11:44:52Z","publication":"Nature Plants","has_accepted_license":"1","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"type":"journal_article","publist_id":"7777","article_processing_charge":"No","article_type":"original","related_material":{"link":[{"url":"https://ist.ac.at/en/news/new-process-in-root-development-discovered/","description":"News on IST Homepage","relation":"press_release"}]},"external_id":{"pmid":["30061750"],"isi":["000443861300016"]},"quality_controlled":"1","volume":4,"day":"30","pmid":1,"file":[{"date_created":"2019-11-18T16:24:07Z","file_name":"2018_NaturePlants_Shi.pdf","date_updated":"2020-07-14T12:44:56Z","access_level":"open_access","creator":"dernst","file_id":"7043","checksum":"da33101c76ee1b2dc5ab28fd2ccba9d0","file_size":226829,"content_type":"application/pdf","relation":"main_file"}],"author":[{"full_name":"Shi, Chun Lin","last_name":"Shi","first_name":"Chun Lin"},{"last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","first_name":"Daniel","full_name":"Von Wangenheim, Daniel"},{"full_name":"Herrmann, Ullrich","last_name":"Herrmann","first_name":"Ullrich"},{"full_name":"Wildhagen, Mari","first_name":"Mari","last_name":"Wildhagen"},{"first_name":"Ivan","last_name":"Kulik","id":"F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB","full_name":"Kulik, Ivan"},{"full_name":"Kopf, Andreas","last_name":"Kopf","first_name":"Andreas"},{"full_name":"Ishida, Takashi","first_name":"Takashi","last_name":"Ishida"},{"first_name":"Vilde","last_name":"Olsson","full_name":"Olsson, Vilde"},{"full_name":"Anker, Mari Kristine","last_name":"Anker","first_name":"Mari Kristine"},{"full_name":"Albert, Markus","last_name":"Albert","first_name":"Markus"},{"first_name":"Melinka A","last_name":"Butenko","full_name":"Butenko, Melinka A"},{"full_name":"Felix, Georg","last_name":"Felix","first_name":"Georg"},{"full_name":"Sawa, Shinichiro","first_name":"Shinichiro","last_name":"Sawa"},{"first_name":"Manfred","last_name":"Claassen","full_name":"Claassen, Manfred"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí"},{"last_name":"Aalen","first_name":"Reidunn B","full_name":"Aalen, Reidunn B"}],"isi":1,"title":"The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling","publisher":"Nature Publishing Group","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1"},{"has_accepted_license":"1","publication":"Journal of visualized experiments JoVE","date_created":"2018-12-11T11:50:01Z","type":"journal_article","department":[{"_id":"JiFr"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"article_processing_charge":"No","pubrep_id":"808","publist_id":"6302","volume":2017,"external_id":{"isi":["000397847200041"]},"related_material":{"record":[{"status":"public","relation":"popular_science","id":"5565"}]},"day":"18","author":[{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"isi":1,"project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"file":[{"file_size":57678,"relation":"main_file","content_type":"application/pdf","date_created":"2018-12-12T10:16:31Z","file_name":"IST-2017-808-v1+1_2017_VWangenheim_list.pdf","date_updated":"2018-12-12T10:16:31Z","access_level":"open_access","creator":"system","file_id":"5219"},{"content_type":"application/pdf","relation":"main_file","file_size":1317820,"date_updated":"2018-12-12T10:16:32Z","file_name":"IST-2017-808-v1+2_2017_VWangenheim_article.pdf","date_created":"2018-12-12T10:16:32Z","creator":"system","file_id":"5220","access_level":"open_access"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Journal of Visualized Experiments","title":"Light sheet fluorescence microscopy of plant roots growing on the surface of a gel","doi":"10.3791/55044","publication_status":"published","oa_version":"Published Version","date_published":"2017-01-18T00:00:00Z","scopus_import":"1","file_date_updated":"2018-12-12T10:16:32Z","ddc":["580"],"citation":{"ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy of plant roots growing on the surface of a gel,” <i>Journal of visualized experiments JoVE</i>, vol. 2017, no. 119. Journal of Visualized Experiments, 2017.","ama":"von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of visualized experiments JoVE</i>. 2017;2017(119). doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>","short":"D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments JoVE 2017 (2017).","mla":"von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>, vol. 2017, no. 119, e55044, Journal of Visualized Experiments, 2017, doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>.","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of visualized experiments JoVE. 2017(119), e55044.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments, 2017. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>."},"intvolume":"      2017","abstract":[{"text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. ","lang":"eng"}],"issue":"119","date_updated":"2025-05-07T11:12:33Z","year":"2017","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"article_number":"e55044","_id":"1078","status":"public","oa":1,"month":"01","ec_funded":1},{"issue":"17","abstract":[{"text":"Plants are sessile organisms rooted in one place. The soil resources that plants require are often distributed in a highly heterogeneous pattern. To aid foraging, plants have evolved roots whose growth and development are highly responsive to soil signals. As a result, 3D root architecture is shaped by myriad environmental signals to ensure resource capture is optimised and unfavourable environments are avoided. The first signals sensed by newly germinating seeds — gravity and light — direct root growth into the soil to aid seedling establishment. Heterogeneous soil resources, such as water, nitrogen and phosphate, also act as signals that shape 3D root growth to optimise uptake. Root architecture is also modified through biotic interactions that include soil fungi and neighbouring plants. This developmental plasticity results in a ‘custom-made’ 3D root system that is best adapted to forage for resources in each soil environment that a plant colonises.","lang":"eng"}],"date_updated":"2021-01-12T08:12:29Z","year":"2017","_id":"722","status":"public","oa":1,"publication_identifier":{"issn":["09609822"]},"month":"09","ec_funded":1,"doi":"10.1016/j.cub.2017.06.043","publication_status":"published","oa_version":"Submitted Version","date_published":"2017-09-11T00:00:00Z","scopus_import":1,"file_date_updated":"2020-07-14T12:47:54Z","ddc":["581"],"citation":{"ista":"Morris E, Griffiths M, Golebiowska A, Mairhofer S, Burr Hersey J, Goh T, von Wangenheim D, Atkinson B, Sturrock C, Lynch J, Vissenberg K, Ritz K, Wells D, Mooney S, Bennett M. 2017. Shaping 3D root system architecture. Current Biology. 27(17), R919–R930.","apa":"Morris, E., Griffiths, M., Golebiowska, A., Mairhofer, S., Burr Hersey, J., Goh, T., … Bennett, M. (2017). Shaping 3D root system architecture. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">https://doi.org/10.1016/j.cub.2017.06.043</a>","mla":"Morris, Emily, et al. “Shaping 3D Root System Architecture.” <i>Current Biology</i>, vol. 27, no. 17, Cell Press, 2017, pp. R919–30, doi:<a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">10.1016/j.cub.2017.06.043</a>.","chicago":"Morris, Emily, Marcus Griffiths, Agata Golebiowska, Stefan Mairhofer, Jasmine Burr Hersey, Tatsuaki Goh, Daniel von Wangenheim, et al. “Shaping 3D Root System Architecture.” <i>Current Biology</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">https://doi.org/10.1016/j.cub.2017.06.043</a>.","ieee":"E. Morris <i>et al.</i>, “Shaping 3D root system architecture,” <i>Current Biology</i>, vol. 27, no. 17. Cell Press, pp. R919–R930, 2017.","ama":"Morris E, Griffiths M, Golebiowska A, et al. Shaping 3D root system architecture. <i>Current Biology</i>. 2017;27(17):R919-R930. doi:<a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">10.1016/j.cub.2017.06.043</a>","short":"E. Morris, M. Griffiths, A. Golebiowska, S. Mairhofer, J. Burr Hersey, T. Goh, D. von Wangenheim, B. Atkinson, C. Sturrock, J. Lynch, K. Vissenberg, K. Ritz, D. Wells, S. Mooney, M. Bennett, Current Biology 27 (2017) R919–R930."},"intvolume":"        27","pmid":1,"day":"11","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"author":[{"last_name":"Morris","first_name":"Emily","full_name":"Morris, Emily"},{"last_name":"Griffiths","first_name":"Marcus","full_name":"Griffiths, Marcus"},{"full_name":"Golebiowska, Agata","last_name":"Golebiowska","first_name":"Agata"},{"first_name":"Stefan","last_name":"Mairhofer","full_name":"Mairhofer, Stefan"},{"full_name":"Burr Hersey, Jasmine","first_name":"Jasmine","last_name":"Burr Hersey"},{"full_name":"Goh, Tatsuaki","last_name":"Goh","first_name":"Tatsuaki"},{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel"},{"full_name":"Atkinson, Brian","first_name":"Brian","last_name":"Atkinson"},{"full_name":"Sturrock, Craig","first_name":"Craig","last_name":"Sturrock"},{"full_name":"Lynch, Jonathan","last_name":"Lynch","first_name":"Jonathan"},{"full_name":"Vissenberg, Kris","first_name":"Kris","last_name":"Vissenberg"},{"full_name":"Ritz, Karl","last_name":"Ritz","first_name":"Karl"},{"last_name":"Wells","first_name":"Darren","full_name":"Wells, Darren"},{"last_name":"Mooney","first_name":"Sacha","full_name":"Mooney, Sacha"},{"full_name":"Bennett, Malcolm","first_name":"Malcolm","last_name":"Bennett"}],"project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"}],"file":[{"date_updated":"2020-07-14T12:47:54Z","file_name":"2017_CurrentBiology_Morris.pdf","date_created":"2019-04-17T07:46:40Z","file_id":"6332","checksum":"e45588b21097b408da6276a3e5eedb2e","creator":"dernst","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_size":1576593}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Cell Press","title":"Shaping 3D root system architecture","has_accepted_license":"1","publication":"Current Biology","date_created":"2018-12-11T11:48:08Z","page":"R919 - R930","type":"journal_article","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"pubrep_id":"982","publist_id":"6956","volume":27,"quality_controlled":"1","external_id":{"pmid":["28898665"]}},{"day":"06","extern":1,"year":"2017","date_updated":"2021-01-12T08:01:23Z","acknowledgement":"Biotechnology and Biological Sciences Research Council:\tBBSRC BB/M001806/1 and BB/H020314/1\t","issue":"5","abstract":[{"lang":"eng","text":"The Casparian strip is an important barrier regulating water and nutrient uptake into root tissues. New research reveals two peptide signals and their co-receptors play critical roles patterning and maintaining barrier integrity. "}],"file":[{"file_size":2840413,"content_type":"application/pdf","relation":"main_file","date_created":"2018-12-12T10:18:11Z","date_updated":"2020-07-14T12:46:38Z","file_name":"IST-2018-983-v1+1_Plant_biology_Building_barriers__in_roots.pdf","access_level":"open_access","creator":"system","file_id":"5330","checksum":"81fd4475c5a2a2c6f4313beeab215ed9"}],"_id":"525","author":[{"first_name":"Daniel","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","full_name":"Daniel von Wangenheim"},{"full_name":"Goh, Tatsuaki","first_name":"Tatsuaki","last_name":"Goh"},{"last_name":"Dietrich","first_name":"Daniela","full_name":"Dietrich, Daniela"},{"first_name":"Malcolm","last_name":"Bennett","full_name":"Bennett, Malcolm J"}],"title":"Plant biology: Building barriers… in roots","oa":1,"publisher":"Cell Press","status":"public","month":"03","date_created":"2018-12-11T11:46:58Z","page":"R172 - R174","publication":"Current Biology","publication_status":"published","doi":"10.1016/j.cub.2017.01.060","date_published":"2017-03-06T00:00:00Z","type":"journal_article","file_date_updated":"2020-07-14T12:46:38Z","publist_id":"7294","main_file_link":[{"open_access":"1","url":"https://repository.ist.ac.at/id/eprint/983"}],"pubrep_id":"983","volume":27,"citation":{"ista":"von Wangenheim D, Goh T, Dietrich D, Bennett M. 2017. Plant biology: Building barriers… in roots. Current Biology. 27(5), R172–R174.","apa":"von Wangenheim, D., Goh, T., Dietrich, D., &#38; Bennett, M. (2017). Plant biology: Building barriers… in roots. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2017.01.060\">https://doi.org/10.1016/j.cub.2017.01.060</a>","mla":"von Wangenheim, Daniel, et al. “Plant Biology: Building Barriers… in Roots.” <i>Current Biology</i>, vol. 27, no. 5, Cell Press, 2017, pp. R172–74, doi:<a href=\"https://doi.org/10.1016/j.cub.2017.01.060\">10.1016/j.cub.2017.01.060</a>.","chicago":"Wangenheim, Daniel von, Tatsuaki Goh, Daniela Dietrich, and Malcolm Bennett. “Plant Biology: Building Barriers… in Roots.” <i>Current Biology</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cub.2017.01.060\">https://doi.org/10.1016/j.cub.2017.01.060</a>.","ama":"von Wangenheim D, Goh T, Dietrich D, Bennett M. Plant biology: Building barriers… in roots. <i>Current Biology</i>. 2017;27(5):R172-R174. doi:<a href=\"https://doi.org/10.1016/j.cub.2017.01.060\">10.1016/j.cub.2017.01.060</a>","ieee":"D. von Wangenheim, T. Goh, D. Dietrich, and M. Bennett, “Plant biology: Building barriers… in roots,” <i>Current Biology</i>, vol. 27, no. 5. Cell Press, pp. R172–R174, 2017.","short":"D. von Wangenheim, T. Goh, D. Dietrich, M. Bennett, Current Biology 27 (2017) R172–R174."},"intvolume":"        27","quality_controlled":0},{"oa":1,"title":"Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institute of Science and Technology Austria","status":"public","ec_funded":1,"month":"04","year":"2017","day":"10","acknowledgement":"fund: FP7-ERC 0101109","abstract":[{"lang":"eng","text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. \r\nThe Video is licensed under a CC BY NC ND license. "}],"date_updated":"2025-05-07T11:12:33Z","_id":"5565","file":[{"file_size":101497758,"relation":"main_file","content_type":"video/mp4","access_level":"open_access","creator":"system","file_id":"5599","checksum":"b7552fc23540a85dc5a22fd4484eae71","date_created":"2018-12-12T13:02:33Z","file_name":"IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4","date_updated":"2020-07-14T12:47:03Z"}],"author":[{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","orcid":"0000-0002-6862-1247","first_name":"Daniel","full_name":"Von Wangenheim, Daniel"},{"full_name":"Hauschild, Robert","first_name":"Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí"}],"project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"ddc":["580"],"article_processing_charge":"No","publist_id":"6302","file_date_updated":"2020-07-14T12:47:03Z","related_material":{"record":[{"id":"1078","status":"public","relation":"research_paper"}]},"citation":{"mla":"von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>.","short":"D. von Wangenheim, R. Hauschild, J. Friml, (2017).","ama":"von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017."},"datarep_id":"66","date_created":"2018-12-12T12:31:34Z","doi":"10.15479/AT:ISTA:66","has_accepted_license":"1","date_published":"2017-04-10T00:00:00Z","department":[{"_id":"JiFr"},{"_id":"Bio"}],"oa_version":"Published Version","type":"research_data"},{"year":"2017","abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"date_updated":"2025-05-07T11:12:33Z","_id":"946","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"article_number":"e26792","oa":1,"status":"public","ec_funded":1,"month":"06","doi":"10.7554/eLife.26792","publication_status":"published","date_published":"2017-06-19T00:00:00Z","oa_version":"Published Version","ddc":["570"],"file_date_updated":"2020-07-14T12:48:15Z","scopus_import":"1","intvolume":"         6","citation":{"ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>."},"day":"19","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","file":[{"access_level":"open_access","file_id":"5315","checksum":"9af3398cb0d81f99d79016a616df22e9","creator":"system","date_created":"2018-12-12T10:17:57Z","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","date_updated":"2020-07-14T12:48:15Z","file_size":19581847,"relation":"main_file","content_type":"application/pdf"}],"author":[{"orcid":"0000-0002-6862-1247","first_name":"Daniel","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","full_name":"Von Wangenheim, Daniel"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"first_name":"Matyas","orcid":"0000-0002-9767-8699","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas"},{"orcid":"0000-0003-2676-3367","first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí"}],"isi":1,"project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"},{"_id":"2572ED28-B435-11E9-9278-68D0E5697425","grant_number":"M02128","name":"Molecular basis of root growth inhibition by auxin","call_identifier":"FWF"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16","call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development"},{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"eLife Sciences Publications","publication":"eLife","date_created":"2018-12-11T11:49:21Z","has_accepted_license":"1","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"type":"journal_article","pubrep_id":"847","article_processing_charge":"Yes","publist_id":"6471","related_material":{"record":[{"status":"public","relation":"popular_science","id":"5566"}]},"quality_controlled":"1","volume":6,"external_id":{"isi":["000404728300001"]}},{"_id":"526","author":[{"full_name":"Daniel von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Fangerau","first_name":"Jens","full_name":"Fangerau, Jens"},{"first_name":"Alexander","last_name":"Schmitz","full_name":"Schmitz, Alexander"},{"first_name":"Richard","last_name":"Smith","full_name":"Smith, Richard S"},{"first_name":"Heike","last_name":"Leitte","full_name":"Leitte, Heike"},{"full_name":"Stelzer, Ernst H","last_name":"Stelzer","first_name":"Ernst"},{"first_name":"Alexis","last_name":"Maizel","full_name":"Maizel, Alexis"}],"extern":1,"year":"2016","day":"22","acknowledgement":"We thank M.J. Bennett, L. Laplaze, and S. Lemke for their helpful comments.\nThis work was supported by the Land Baden-Württemberg, the Chica und Heinz Schaller Stiftung, the CellNetworks cluster of excellence, and the Boehringer Ingelheim Fond (to J.F. and A.M.) and the Cluster of Excellence “Macromolecular Complexes” at the Goethe University Frankfurt am Main (CEF-MC II; DFG Project EXC 115; to D.v.W., A.S., and E.H.K.S.).\n","issue":"4","abstract":[{"text":"Plants form new organs with patterned tissue organization throughout their lifespan. It is unknown whether this robust post-embryonic organ formation results from stereotypic dynamic processes, in which the arrangement of cells follows rigid rules. Here, we combine modeling with empirical observations of whole-organ development to identify the principles governing lateral root formation in Arabidopsis. Lateral roots derive from a small pool of founder cells in which some take a dominant role as seen by lineage tracing. The first division of the founders is asymmetric, tightly regulated, and determines the formation of a layered structure. Whereas the pattern of subsequent cell divisions is not stereotypic between different samples, it is characterized by a regular switch in division plane orientation. This switch is also necessary for the appearance of patterned layers as a result of the apical growth of the primordium. Our data suggest that lateral root morphogenesis is based on a limited set of rules. They determine cell growth and division orientation. The organ-level coupling of the cell behavior ensures the emergence of the lateral root's characteristic features. We propose that self-organizing, non-deterministic modes of development account for the robustness of plant organ morphogenesis.","lang":"eng"}],"date_updated":"2021-01-12T08:01:24Z","month":"02","title":"Rules and self-organizing properties of post-embryonic plant organ cell division patterns","publisher":"Cell Press","status":"public","date_published":"2016-02-22T00:00:00Z","type":"journal_article","publication":"Current Biology","page":"439 - 449","date_created":"2018-12-11T11:46:58Z","doi":"10.1016/j.cub.2015.12.047","publication_status":"published","quality_controlled":0,"volume":26,"intvolume":"        26","citation":{"ista":"von Wangenheim D, Fangerau J, Schmitz A, Smith R, Leitte H, Stelzer E, Maizel A. 2016. Rules and self-organizing properties of post-embryonic plant organ cell division patterns. Current Biology. 26(4), 439–449.","mla":"von Wangenheim, Daniel, et al. “Rules and Self-Organizing Properties of Post-Embryonic Plant Organ Cell Division Patterns.” <i>Current Biology</i>, vol. 26, no. 4, Cell Press, 2016, pp. 439–49, doi:<a href=\"https://doi.org/10.1016/j.cub.2015.12.047\">10.1016/j.cub.2015.12.047</a>.","apa":"von Wangenheim, D., Fangerau, J., Schmitz, A., Smith, R., Leitte, H., Stelzer, E., &#38; Maizel, A. (2016). Rules and self-organizing properties of post-embryonic plant organ cell division patterns. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2015.12.047\">https://doi.org/10.1016/j.cub.2015.12.047</a>","chicago":"Wangenheim, Daniel von, Jens Fangerau, Alexander Schmitz, Richard Smith, Heike Leitte, Ernst Stelzer, and Alexis Maizel. “Rules and Self-Organizing Properties of Post-Embryonic Plant Organ Cell Division Patterns.” <i>Current Biology</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.cub.2015.12.047\">https://doi.org/10.1016/j.cub.2015.12.047</a>.","short":"D. von Wangenheim, J. Fangerau, A. Schmitz, R. Smith, H. Leitte, E. Stelzer, A. Maizel, Current Biology 26 (2016) 439–449.","ama":"von Wangenheim D, Fangerau J, Schmitz A, et al. Rules and self-organizing properties of post-embryonic plant organ cell division patterns. <i>Current Biology</i>. 2016;26(4):439-449. doi:<a href=\"https://doi.org/10.1016/j.cub.2015.12.047\">10.1016/j.cub.2015.12.047</a>","ieee":"D. von Wangenheim <i>et al.</i>, “Rules and self-organizing properties of post-embryonic plant organ cell division patterns,” <i>Current Biology</i>, vol. 26, no. 4. Cell Press, pp. 439–449, 2016."},"publist_id":"7293"},{"citation":{"ieee":"D. von Wangenheim <i>et al.</i>, “Endosomal interactions during root hair growth,” <i>Frontiers in Plant Science</i>, vol. 6, no. JAN2016. Frontiers Research Foundation, 2016.","ama":"von Wangenheim D, Rosero A, Komis G, et al. Endosomal interactions during root hair growth. <i>Frontiers in Plant Science</i>. 2016;6(JAN2016). doi:<a href=\"https://doi.org/10.3389/fpls.2015.01262\">10.3389/fpls.2015.01262</a>","short":"D. von Wangenheim, A. Rosero, G. Komis, O. Šamajová, M. Ovečka, B. Voigt, J. Šamaj, Frontiers in Plant Science 6 (2016).","ista":"von Wangenheim D, Rosero A, Komis G, Šamajová O, Ovečka M, Voigt B, Šamaj J. 2016. Endosomal interactions during root hair growth. Frontiers in Plant Science. 6(JAN2016), 1262.","mla":"von Wangenheim, Daniel, et al. “Endosomal Interactions during Root Hair Growth.” <i>Frontiers in Plant Science</i>, vol. 6, no. JAN2016, 1262, Frontiers Research Foundation, 2016, doi:<a href=\"https://doi.org/10.3389/fpls.2015.01262\">10.3389/fpls.2015.01262</a>.","apa":"von Wangenheim, D., Rosero, A., Komis, G., Šamajová, O., Ovečka, M., Voigt, B., &#38; Šamaj, J. (2016). Endosomal interactions during root hair growth. <i>Frontiers in Plant Science</i>. Frontiers Research Foundation. <a href=\"https://doi.org/10.3389/fpls.2015.01262\">https://doi.org/10.3389/fpls.2015.01262</a>","chicago":"Wangenheim, Daniel von, Amparo Rosero, George Komis, Olga Šamajová, Miroslav Ovečka, Boris Voigt, and Jozef Šamaj. “Endosomal Interactions during Root Hair Growth.” <i>Frontiers in Plant Science</i>. Frontiers Research Foundation, 2016. <a href=\"https://doi.org/10.3389/fpls.2015.01262\">https://doi.org/10.3389/fpls.2015.01262</a>."},"intvolume":"         6","ddc":["581"],"scopus_import":1,"file_date_updated":"2020-07-14T12:44:41Z","date_published":"2016-01-29T00:00:00Z","oa_version":"Published Version","doi":"10.3389/fpls.2015.01262","publication_status":"published","month":"01","oa":1,"status":"public","_id":"1238","article_number":"1262","year":"2016","issue":"JAN2016","abstract":[{"lang":"eng","text":"The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore, we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Our results show differential motile behavior of early and late endosomes and interactions of late endosomes that may be specified to particular root hair domains. Detailed data analysis revealed a particular transient interaction between late endosomes—termed herein as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth."}],"date_updated":"2021-01-12T06:49:18Z","quality_controlled":"1","volume":6,"pubrep_id":"710","publist_id":"6094","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"type":"journal_article","publication":"Frontiers in Plant Science","date_created":"2018-12-11T11:50:53Z","has_accepted_license":"1","title":"Endosomal interactions during root hair growth","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"Frontiers Research Foundation","file":[{"file_size":1640550,"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_id":"4760","checksum":"3127eab844d53564bf47e2b6b42f1ca0","creator":"system","date_created":"2018-12-12T10:09:36Z","date_updated":"2020-07-14T12:44:41Z","file_name":"IST-2016-710-v1+1_fpls-06-01262.pdf"}],"author":[{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel"},{"last_name":"Rosero","first_name":"Amparo","full_name":"Rosero, Amparo"},{"last_name":"Komis","first_name":"George","full_name":"Komis, George"},{"full_name":"Šamajová, Olga","first_name":"Olga","last_name":"Šamajová"},{"full_name":"Ovečka, Miroslav","last_name":"Ovečka","first_name":"Miroslav"},{"full_name":"Voigt, Boris","first_name":"Boris","last_name":"Voigt"},{"full_name":"Šamaj, Jozef","last_name":"Šamaj","first_name":"Jozef"}],"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"day":"29","acknowledgement":"This work was supported by National Program for Sustainability I (grant no. LO1204) provided by the Czech Ministry of Education and by Institutional Fund of Palacký University Olomouc (GK and OŠ).\r\nWe thank Sabine Fischer for help with the statistics."},{"month":"05","ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","publisher":"Cell Press","title":"An auxin transport mechanism restricts positive orthogravitropism in lateral roots","author":[{"full_name":"Rosquete, Michel","first_name":"Michel","last_name":"Rosquete"},{"full_name":"Von Wangenheim, Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim","orcid":"0000-0002-6862-1247","first_name":"Daniel"},{"full_name":"Marhavy, Peter","last_name":"Marhavy","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","first_name":"Peter"},{"full_name":"Barbez, Elke","last_name":"Barbez","first_name":"Elke"},{"full_name":"Stelzer, Ernst","first_name":"Ernst","last_name":"Stelzer"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva"},{"first_name":"Alexis","last_name":"Maizel","full_name":"Maizel, Alexis"},{"full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"}],"project":[{"call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis","grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"}],"_id":"2844","abstract":[{"text":"As soon as a seed germinates, plant growth relates to gravity to ensure that the root penetrates the soil and the shoot expands aerially. Whereas mechanisms of positive and negative orthogravitropism of primary roots and shoots are relatively well understood [1-3], lateral organs often show more complex growth behavior [4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show a defined gravitropic set-point angle (GSA) that allows radial expansion of the root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture, it so far remains completely unknown how lateral organs partially suppress positive orthogravitropism. Here we show that the phytohormone auxin steers GSA formation and limits positive orthogravitropism in LR. Low and high auxin levels/signaling lead to radial or axial root systems, respectively. At a cellular level, it is the auxin transport-dependent regulation of asymmetric growth in the elongation zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7 and transient PIN3 expression limit auxin redistribution in young LR columella cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin fluxes in the tip of LRs, induces transient, differential growth responses in the elongation zone and, consequently, controls root architecture.","lang":"eng"}],"issue":"9","date_updated":"2021-01-12T07:00:10Z","year":"2013","day":"06","volume":23,"citation":{"short":"M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková, A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.","ieee":"M. Rosquete <i>et al.</i>, “An auxin transport mechanism restricts positive orthogravitropism in lateral roots,” <i>Current Biology</i>, vol. 23, no. 9. Cell Press, pp. 817–822, 2013.","ama":"Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. 2013;23(9):817-822. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>","chicago":"Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>.","ista":"Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 23(9), 817–822.","mla":"Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>, vol. 23, no. 9, Cell Press, 2013, pp. 817–22, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>.","apa":"Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková, E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>"},"quality_controlled":"1","intvolume":"        23","publist_id":"3950","scopus_import":1,"oa_version":"None","type":"journal_article","date_published":"2013-05-06T00:00:00Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.cub.2013.03.064","publication_status":"published","publication":"Current Biology","date_created":"2018-12-11T11:59:53Z","page":"817 - 822"}]