---
_id: '676'
abstract:
- lang: eng
text: The segregation of different cell types into distinct tissues is a fundamental
process in metazoan development. Differences in cell adhesion and cortex tension
are commonly thought to drive cell sorting by regulating tissue surface tension
(TST). However, the role that differential TST plays in cell segregation within
the developing embryo is as yet unclear. Here, we have analyzed the role of differential
TST for germ layer progenitor cell segregation during zebrafish gastrulation.
Contrary to previous observations that differential TST drives germ layer progenitor
cell segregation in vitro, we show that germ layers display indistinguishable
TST within the gastrulating embryo, arguing against differential TST driving germ
layer progenitor cell segregation in vivo. We further show that the osmolarity
of the interstitial fluid (IF) is an important factor that influences germ layer
TST in vivo, and that lower osmolarity of the IF compared with standard cell culture
medium can explain why germ layers display differential TST in culture but not
in vivo. Finally, we show that directed migration of mesendoderm progenitors is
required for germ layer progenitor cell segregation and germ layer formation.
article_processing_charge: No
article_type: original
author:
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Jim
full_name: Veldhuis, Jim
last_name: Veldhuis
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Daniel
full_name: Capek, Daniel
id: 31C42484-F248-11E8-B48F-1D18A9856A87
last_name: Capek
orcid: 0000-0001-5199-9940
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Wayne
full_name: Brodland, Wayne
last_name: Brodland
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates
the action of differential tissue surface tension in progenitor cell segregation
during gastrulation. Development. 2017;144(10):1798-1806. doi:10.1242/dev.144964
apa: Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W.,
& Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the
action of differential tissue surface tension in progenitor cell segregation during
gastrulation. Development. Company of Biologists. https://doi.org/10.1242/dev.144964
chicago: Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître,
Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity
Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell
Segregation during Gastrulation.” Development. Company of Biologists, 2017.
https://doi.org/10.1242/dev.144964.
ieee: G. Krens et al., “Interstitial fluid osmolarity modulates the action
of differential tissue surface tension in progenitor cell segregation during gastrulation,”
Development, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.
ista: Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg
C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential
tissue surface tension in progenitor cell segregation during gastrulation. Development.
144(10), 1798–1806.
mla: Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action
of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.”
Development, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806,
doi:10.1242/dev.144964.
short: G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J.
Heisenberg, Development 144 (2017) 1798–1806.
date_created: 2018-12-11T11:47:52Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2024-03-25T23:30:13Z
day: '15'
ddc:
- '570'
department:
- _id: Bio
- _id: CaHe
doi: 10.1242/dev.144964
external_id:
pmid:
- '28512197'
file:
- access_level: open_access
checksum: bc25125fb664706cdf180e061429f91d
content_type: application/pdf
creator: dernst
date_created: 2019-09-24T06:56:22Z
date_updated: 2020-07-14T12:47:39Z
file_id: '6905'
file_name: 2017_Development_Krens.pdf
file_size: 8194516
relation: main_file
file_date_updated: 2020-07-14T12:47:39Z
has_accepted_license: '1'
intvolume: ' 144'
issue: '10'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1798 - 1806
pmid: 1
publication: Development
publication_identifier:
issn:
- '09501991'
publication_status: published
publisher: Company of Biologists
publist_id: '7047'
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related_material:
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relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Interstitial fluid osmolarity modulates the action of differential tissue surface
tension in progenitor cell segregation during gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 144
year: '2017'
...
---
_id: '1923'
abstract:
- lang: eng
text: We derive the equations for a thin, axisymmetric elastic shell subjected to
an internal active stress giving rise to active tension and moments within the
shell. We discuss the stability of a cylindrical elastic shell and its response
to a localized change in internal active stress. This description is relevant
to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving
elastically at a short timescale and subjected to active internal forces arising
from myosin molecular motor activity. We show that the recent observations of
cell deformation following detachment of adherent cells (Maître J-L et al 2012
Science 338 253-6) are well accounted for by this mechanical description. The
actin cortex elastic and bending moduli can be obtained from a quantitative analysis
of cell shapes observed in these experiments. Our approach thus provides a non-invasive,
imaging-based method for the extraction of cellular physical parameters.
article_number: '065005'
author:
- first_name: Hélène
full_name: Berthoumieux, Hélène
last_name: Berthoumieux
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
citation:
ama: Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux
G. Active elastic thin shell theory for cellular deformations. New Journal
of Physics. 2014;16. doi:10.1088/1367-2630/16/6/065005
apa: Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher,
F., & Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations.
New Journal of Physics. IOP Publishing Ltd. https://doi.org/10.1088/1367-2630/16/6/065005
chicago: Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa
Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory
for Cellular Deformations.” New Journal of Physics. IOP Publishing Ltd.,
2014. https://doi.org/10.1088/1367-2630/16/6/065005.
ieee: H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher,
and G. Salbreux, “Active elastic thin shell theory for cellular deformations,”
New Journal of Physics, vol. 16. IOP Publishing Ltd., 2014.
ista: Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux
G. 2014. Active elastic thin shell theory for cellular deformations. New Journal
of Physics. 16, 065005.
mla: Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular
Deformations.” New Journal of Physics, vol. 16, 065005, IOP Publishing
Ltd., 2014, doi:10.1088/1367-2630/16/6/065005.
short: H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher,
G. Salbreux, New Journal of Physics 16 (2014).
date_created: 2018-12-11T11:54:44Z
date_published: 2014-06-01T00:00:00Z
date_updated: 2021-01-12T06:54:06Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1088/1367-2630/16/6/065005
file:
- access_level: open_access
checksum: 8dbe81ec656bf1264d8889bda9b2b985
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:16Z
date_updated: 2020-07-14T12:45:21Z
file_id: '5202'
file_name: IST-2016-429-v1+1_document.pdf
file_size: 941387
relation: main_file
file_date_updated: 2020-07-14T12:45:21Z
has_accepted_license: '1'
intvolume: ' 16'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: New Journal of Physics
publication_status: published
publisher: IOP Publishing Ltd.
publist_id: '5171'
pubrep_id: '429'
quality_controlled: '1'
scopus_import: 1
status: public
title: Active elastic thin shell theory for cellular deformations
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2014'
...
---
_id: '2469'
abstract:
- lang: eng
text: Cadherins are transmembrane proteins that mediate cell–cell adhesion in animals.
By regulating contact formation and stability, cadherins play a crucial role in
tissue morphogenesis and homeostasis. Here, we review the three major unctions
of cadherins in cell–cell contact formation and stability. Two of those functions
lead to a decrease in interfacial ension at the forming cell–cell contact, thereby
promoting contact expansion — first, by providing adhesion tension that lowers
interfacial tension at the cell–cell contact, and second, by signaling to the
actomyosin cytoskeleton in order to reduce cortex tension and thus interfacial
tension at the contact. The third function of cadherins in cell–cell contact formation
is to stabilize the contact by resisting mechanical forces that pull on the contact.
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Maître J-L, Heisenberg C-PJ. Three functions of cadherins in cell adhesion.
Current Biology. 2013;23(14):R626-R633. doi:10.1016/j.cub.2013.06.019
apa: Maître, J.-L., & Heisenberg, C.-P. J. (2013). Three functions of cadherins
in cell adhesion. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.06.019
chicago: Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “Three Functions of Cadherins
in Cell Adhesion.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.06.019.
ieee: J.-L. Maître and C.-P. J. Heisenberg, “Three functions of cadherins in cell
adhesion,” Current Biology, vol. 23, no. 14. Cell Press, pp. R626–R633,
2013.
ista: Maître J-L, Heisenberg C-PJ. 2013. Three functions of cadherins in cell adhesion.
Current Biology. 23(14), R626–R633.
mla: Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “Three Functions of Cadherins
in Cell Adhesion.” Current Biology, vol. 23, no. 14, Cell Press, 2013,
pp. R626–33, doi:10.1016/j.cub.2013.06.019.
short: J.-L. Maître, C.-P.J. Heisenberg, Current Biology 23 (2013) R626–R633.
date_created: 2018-12-11T11:57:51Z
date_published: 2013-07-22T00:00:00Z
date_updated: 2021-01-12T06:57:40Z
day: '22'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1016/j.cub.2013.06.019
external_id:
pmid:
- '23885883'
file:
- access_level: open_access
checksum: 6a424b2f007b41d4955a9135793b2162
content_type: application/pdf
creator: dernst
date_created: 2019-01-24T15:40:22Z
date_updated: 2020-07-14T12:45:41Z
file_id: '5881'
file_name: 2013_CurrentBiology_Maitre.pdf
file_size: 247320
relation: main_file
file_date_updated: 2020-07-14T12:45:41Z
has_accepted_license: '1'
intvolume: ' 23'
issue: '14'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: R626 - R633
pmid: 1
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '4433'
quality_controlled: '1'
scopus_import: 1
status: public
title: Three functions of cadherins in cell adhesion
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2013'
...
---
_id: '2884'
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Hélène
full_name: Berthoumieux, Hélène
last_name: Berthoumieux
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish
gastrulation. Medecine Sciences. 2013;29(2):147-150. doi:10.1051/medsci/2013292011
apa: Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch,
E., & Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation.
Medecine Sciences. Éditions Médicales et Scientifiques. https://doi.org/10.1051/medsci/2013292011
chicago: Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux,
Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics
of Zebrafish Gastrulation.” Medecine Sciences. Éditions Médicales et Scientifiques,
2013. https://doi.org/10.1051/medsci/2013292011.
ieee: J.-L. Maître et al., “Cell adhesion mechanics of zebrafish gastrulation,”
Medecine Sciences, vol. 29, no. 2. Éditions Médicales et Scientifiques,
pp. 147–150, 2013.
ista: Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg
C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences.
29(2), 147–150.
mla: Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.”
Medecine Sciences, vol. 29, no. 2, Éditions Médicales et Scientifiques,
2013, pp. 147–50, doi:10.1051/medsci/2013292011.
short: J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch,
C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150.
date_created: 2018-12-11T12:00:08Z
date_published: 2013-02-01T00:00:00Z
date_updated: 2021-01-12T07:00:28Z
day: '01'
department:
- _id: CaHe
doi: 10.1051/medsci/2013292011
intvolume: ' 29'
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 147 - 150
project:
- _id: 252064B8-B435-11E9-9278-68D0E5697425
grant_number: HE_3231/6-1
name: Analysis of the Formation and Function of Different Cell Protusion Types During
Cell Migration in Vivo
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 812-B12
name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
publication: Medecine Sciences
publication_status: published
publisher: Éditions Médicales et Scientifiques
publist_id: '3877'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cell adhesion mechanics of zebrafish gastrulation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2013'
...
---
_id: '2951'
abstract:
- lang: eng
text: Differential cell adhesion and cortex tension are thought to drive cell sorting
by controlling cell-cell contact formation. Here, we show that cell adhesion and
cortex tension have different mechanical functions in controlling progenitor cell-cell
contact formation and sorting during zebrafish gastrulation. Cortex tension controls
cell-cell contact expansion by modulating interfacial tension at the contact.
By contrast, adhesion has little direct function in contact expansion, but instead
is needed to mechanically couple the cortices of adhering cells at their contacts,
allowing cortex tension to control contact expansion. The coupling function of
adhesion is mediated by E-cadherin and limited by the mechanical anchoring of
E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold
for cell cortex tension to drive cell sorting during gastrulation.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Hélène
full_name: Berthoumieux, Hélène
last_name: Berthoumieux
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting
by mechanically coupling the cortices of adhering cells. Science. 2012;338(6104):253-256.
doi:10.1126/science.1225399
apa: Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch,
E., & Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically
coupling the cortices of adhering cells. Science. American Association
for the Advancement of Science. https://doi.org/10.1126/science.1225399
chicago: Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux,
Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions
in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” Science.
American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1225399.
ieee: J.-L. Maître et al., “Adhesion functions in cell sorting by mechanically
coupling the cortices of adhering cells,” Science, vol. 338, no. 6104.
American Association for the Advancement of Science, pp. 253–256, 2012.
ista: Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg
C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices
of adhering cells. Science. 338(6104), 253–256.
mla: Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically
Coupling the Cortices of Adhering Cells.” Science, vol. 338, no. 6104,
American Association for the Advancement of Science, 2012, pp. 253–56, doi:10.1126/science.1225399.
short: J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch,
C.-P.J. Heisenberg, Science 338 (2012) 253–256.
date_created: 2018-12-11T12:00:31Z
date_published: 2012-10-12T00:00:00Z
date_updated: 2021-01-12T07:40:00Z
day: '12'
department:
- _id: CaHe
doi: 10.1126/science.1225399
intvolume: ' 338'
issue: '6104'
language:
- iso: eng
month: '10'
oa_version: None
page: 253 - 256
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3777'
quality_controlled: '1'
scopus_import: 1
status: public
title: Adhesion functions in cell sorting by mechanically coupling the cortices of
adhering cells
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 338
year: '2012'
...
---
_id: '3273'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
citation:
ama: Maître J-L. Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors.
2011.
apa: Maître, J.-L. (2011). Mechanics of adhesion and de‐adhesion in zebrafish
germ layer progenitors. Institute of Science and Technology Austria.
chicago: Maître, Jean-Léon. “Mechanics of Adhesion and De‐adhesion in Zebrafish
Germ Layer Progenitors.” Institute of Science and Technology Austria, 2011.
ieee: J.-L. Maître, “Mechanics of adhesion and de‐adhesion in zebrafish germ layer
progenitors,” Institute of Science and Technology Austria, 2011.
ista: Maître J-L. 2011. Mechanics of adhesion and de‐adhesion in zebrafish germ
layer progenitors. Institute of Science and Technology Austria.
mla: Maître, Jean-Léon. Mechanics of Adhesion and De‐adhesion in Zebrafish Germ
Layer Progenitors. Institute of Science and Technology Austria, 2011.
short: J.-L. Maître, Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer
Progenitors, Institute of Science and Technology Austria, 2011.
date_created: 2018-12-11T12:02:23Z
date_published: 2011-12-12T00:00:00Z
date_updated: 2023-09-07T11:30:16Z
day: '12'
degree_awarded: PhD
department:
- _id: CaHe
language:
- iso: eng
month: '12'
oa_version: None
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '3373'
status: public
supervisor:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
title: Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2011'
...
---
_id: '3379'
abstract:
- lang: eng
text: The process of gastrulation is highly conserved across vertebrates on both
the genetic and morphological levels, despite great variety in embryonic shape
and speed of development. This mechanism spatially separates the germ layers and
establishes the organizational foundation for future development. Mesodermal identity
is specified in a superficial layer of cells, the epiblast, where cells maintain
an epithelioid morphology. These cells involute to join the deeper hypoblast layer
where they adopt a migratory, mesenchymal morphology. Expression of a cascade
of related transcription factors orchestrates the parallel genetic transition
from primitive to mature mesoderm. Although the early and late stages of this
process are increasingly well understood, the transition between them has remained
largely mysterious. We present here the first high resolution in vivo observations
of the blebby transitional morphology of involuting mesodermal cells in a vertebrate
embryo. We further demonstrate that the zebrafish spadetail mutation creates a
reversible block in the maturation program, stalling cells in the transition state.
This mutation creates an ideal system for dissecting the specific properties of
cells undergoing the morphological transition of maturing mesoderm, as we demonstrate
with a direct measurement of cell–cell adhesion.
article_type: original
author:
- first_name: Richard
full_name: Row, Richard
last_name: Row
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Benjamin
full_name: Martin, Benjamin
last_name: Martin
- first_name: Petra
full_name: Stockinger, Petra
id: 261CB030-E90D-11E9-B182-F697D44B663C
last_name: Stockinger
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: David
full_name: Kimelman, David
last_name: Kimelman
citation:
ama: Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. Completion
of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail.
Developmental Biology. 2011;354(1):102-110. doi:10.1016/j.ydbio.2011.03.025
apa: Row, R., Maître, J.-L., Martin, B., Stockinger, P., Heisenberg, C.-P. J., &
Kimelman, D. (2011). Completion of the epithelial to mesenchymal transition in
zebrafish mesoderm requires Spadetail. Developmental Biology. Elsevier.
https://doi.org/10.1016/j.ydbio.2011.03.025
chicago: Row, Richard, Jean-Léon Maître, Benjamin Martin, Petra Stockinger, Carl-Philipp
J Heisenberg, and David Kimelman. “Completion of the Epithelial to Mesenchymal
Transition in Zebrafish Mesoderm Requires Spadetail.” Developmental Biology.
Elsevier, 2011. https://doi.org/10.1016/j.ydbio.2011.03.025.
ieee: R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P. J. Heisenberg, and D.
Kimelman, “Completion of the epithelial to mesenchymal transition in zebrafish
mesoderm requires Spadetail,” Developmental Biology, vol. 354, no. 1. Elsevier,
pp. 102–110, 2011.
ista: Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. 2011.
Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires
Spadetail. Developmental Biology. 354(1), 102–110.
mla: Row, Richard, et al. “Completion of the Epithelial to Mesenchymal Transition
in Zebrafish Mesoderm Requires Spadetail.” Developmental Biology, vol.
354, no. 1, Elsevier, 2011, pp. 102–10, doi:10.1016/j.ydbio.2011.03.025.
short: R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P.J. Heisenberg, D. Kimelman,
Developmental Biology 354 (2011) 102–110.
date_created: 2018-12-11T12:03:00Z
date_published: 2011-06-01T00:00:00Z
date_updated: 2021-01-12T07:43:04Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/j.ydbio.2011.03.025
external_id:
pmid:
- '1463614'
intvolume: ' 354'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090540/
month: '06'
oa: 1
oa_version: Submitted Version
page: 102 - 110
pmid: 1
publication: Developmental Biology
publication_status: published
publisher: Elsevier
publist_id: '3228'
quality_controlled: '1'
scopus_import: 1
status: public
title: Completion of the epithelial to mesenchymal transition in zebrafish mesoderm
requires Spadetail
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 354
year: '2011'
...
---
_id: '3396'
abstract:
- lang: eng
text: Facial branchiomotor neurons (FBMNs) in zebrafish and mouse embryonic hindbrain
undergo a characteristic tangential migration from rhombomere (r) 4, where they
are born, to r6/7. Cohesion among neuroepithelial cells (NCs) has been suggested
to function in FBMN migration by inhibiting FBMNs positioned in the basal neuroepithelium
such that they move apically between NCs towards the midline of the neuroepithelium
instead of tangentially along the basal side of the neuroepithelium towards r6/7.
However, direct experimental evaluation of this hypothesis is still lacking. Here,
we have used a combination of biophysical cell adhesion measurements and high-resolution
time-lapse microscopy to determine the role of NC cohesion in FBMN migration.
We show that reducing NC cohesion by interfering with Cadherin 2 (Cdh2) activity
results in FBMNs positioned at the basal side of the neuroepithelium moving apically
towards the neural tube midline instead of tangentially towards r6/7. In embryos
with strongly reduced NC cohesion, ectopic apical FBMN movement frequently results
in fusion of the bilateral FBMN clusters over the apical midline of the neural
tube. By contrast, reducing cohesion among FBMNs by interfering with Contactin
2 (Cntn2) expression in these cells has little effect on apical FBMN movement,
but reduces the fusion of the bilateral FBMN clusters in embryos with strongly
diminished NC cohesion. These data provide direct experimental evidence that NC
cohesion functions in tangential FBMN migration by restricting their apical movement.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
article_type: original
author:
- first_name: Petra
full_name: Stockinger, Petra
id: 261CB030-E90D-11E9-B182-F697D44B663C
last_name: Stockinger
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
citation:
ama: Stockinger P, Heisenberg C-PJ, Maître J-L. Defective neuroepithelial cell cohesion
affects tangential branchiomotor neuron migration in the zebrafish neural tube.
Development. 2011;138(21):4673-4683. doi:10.1242/dev.071233
apa: Stockinger, P., Heisenberg, C.-P. J., & Maître, J.-L. (2011). Defective
neuroepithelial cell cohesion affects tangential branchiomotor neuron migration
in the zebrafish neural tube. Development. Company of Biologists. https://doi.org/10.1242/dev.071233
chicago: Stockinger, Petra, Carl-Philipp J Heisenberg, and Jean-Léon Maître. “Defective
Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration
in the Zebrafish Neural Tube.” Development. Company of Biologists, 2011.
https://doi.org/10.1242/dev.071233.
ieee: P. Stockinger, C.-P. J. Heisenberg, and J.-L. Maître, “Defective neuroepithelial
cell cohesion affects tangential branchiomotor neuron migration in the zebrafish
neural tube,” Development, vol. 138, no. 21. Company of Biologists, pp.
4673–4683, 2011.
ista: Stockinger P, Heisenberg C-PJ, Maître J-L. 2011. Defective neuroepithelial
cell cohesion affects tangential branchiomotor neuron migration in the zebrafish
neural tube. Development. 138(21), 4673–4683.
mla: Stockinger, Petra, et al. “Defective Neuroepithelial Cell Cohesion Affects
Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” Development,
vol. 138, no. 21, Company of Biologists, 2011, pp. 4673–83, doi:10.1242/dev.071233.
short: P. Stockinger, C.-P.J. Heisenberg, J.-L. Maître, Development 138 (2011) 4673–4683.
date_created: 2018-12-11T12:03:06Z
date_published: 2011-09-28T00:00:00Z
date_updated: 2021-01-12T07:43:11Z
day: '28'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1242/dev.071233
file:
- access_level: open_access
checksum: ca12b79e01ef36c1ef1aea31cf7e7139
content_type: application/pdf
creator: dernst
date_created: 2019-10-07T14:19:42Z
date_updated: 2020-07-14T12:46:12Z
file_id: '6930'
file_name: 2011_Development_Stockinger.pdf
file_size: 4672439
relation: main_file
file_date_updated: 2020-07-14T12:46:12Z
has_accepted_license: '1'
intvolume: ' 138'
issue: '21'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 4673 - 4683
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '3210'
quality_controlled: '1'
scopus_import: 1
status: public
title: Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron
migration in the zebrafish neural tube
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 138
year: '2011'
...
---
_id: '3397'
abstract:
- lang: eng
text: Recent advances in microscopy techniques and biophysical measurements have
provided novel insight into the molecular, cellular and biophysical basis of cell
adhesion. However, comparably little is known about a core element of cell–cell
adhesion—the energy of adhesion at the cell–cell contact. In this review, we discuss
approaches to understand the nature and regulation of adhesion energy, and propose
strategies to determine adhesion energy between cells in vitro and in vivo.
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Maître J-L, Heisenberg C-PJ. The role of adhesion energy in controlling cell-cell
contacts. Current Opinion in Cell Biology. 2011;23(5):508-514. doi:10.1016/j.ceb.2011.07.004
apa: Maître, J.-L., & Heisenberg, C.-P. J. (2011). The role of adhesion energy
in controlling cell-cell contacts. Current Opinion in Cell Biology. Elsevier.
https://doi.org/10.1016/j.ceb.2011.07.004
chicago: Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “The Role of Adhesion
Energy in Controlling Cell-Cell Contacts.” Current Opinion in Cell Biology.
Elsevier, 2011. https://doi.org/10.1016/j.ceb.2011.07.004.
ieee: J.-L. Maître and C.-P. J. Heisenberg, “The role of adhesion energy in controlling
cell-cell contacts,” Current Opinion in Cell Biology, vol. 23, no. 5. Elsevier,
pp. 508–514, 2011.
ista: Maître J-L, Heisenberg C-PJ. 2011. The role of adhesion energy in controlling
cell-cell contacts. Current Opinion in Cell Biology. 23(5), 508–514.
mla: Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “The Role of Adhesion Energy
in Controlling Cell-Cell Contacts.” Current Opinion in Cell Biology, vol.
23, no. 5, Elsevier, 2011, pp. 508–14, doi:10.1016/j.ceb.2011.07.004.
short: J.-L. Maître, C.-P.J. Heisenberg, Current Opinion in Cell Biology 23 (2011)
508–514.
date_created: 2018-12-11T12:03:06Z
date_published: 2011-10-01T00:00:00Z
date_updated: 2021-01-12T07:43:12Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/j.ceb.2011.07.004
intvolume: ' 23'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188705/
month: '10'
oa: 1
oa_version: Submitted Version
page: 508 - 514
publication: Current Opinion in Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '3211'
quality_controlled: '1'
scopus_import: 1
status: public
title: The role of adhesion energy in controlling cell-cell contacts
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2011'
...