[{"tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"file":[{"file_size":2366976,"relation":"main_file","content_type":"application/pdf","file_name":"2022_ProceedingsRoyalSocB_Kelemen.pdf","success":1,"creator":"dernst","file_id":"10779","date_created":"2022-02-21T08:17:38Z","date_updated":"2022-02-21T08:17:38Z","access_level":"open_access","checksum":"27042a3706ae52a919fed1ac114bf7bb"}],"article_type":"original","volume":289,"oa_version":"Published Version","page":"20211985","quality_controlled":"1","isi":1,"publication_identifier":{"eissn":["14712954"]},"day":"09","citation":{"short":"R.K. Kelemen, M.N. Elkrewi, A.K. Lindholm, B. Vicoso, Proceedings of the Royal Society B: Biological Sciences 289 (2022) 20211985.","chicago":"Kelemen, Réka K, Marwan N Elkrewi, Anna K. Lindholm, and Beatriz Vicoso. “Novel Patterns of Expression and Recruitment of New Genes on the T-Haplotype, a Mouse Selfish Chromosome.” <i>Proceedings of the Royal Society B: Biological Sciences</i>. The Royal Society, 2022. <a href=\"https://doi.org/10.1098/rspb.2021.1985\">https://doi.org/10.1098/rspb.2021.1985</a>.","ieee":"R. K. Kelemen, M. N. Elkrewi, A. K. Lindholm, and B. Vicoso, “Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome,” <i>Proceedings of the Royal Society B: Biological Sciences</i>, vol. 289, no. 1968. The Royal Society, p. 20211985, 2022.","apa":"Kelemen, R. K., Elkrewi, M. N., Lindholm, A. K., &#38; Vicoso, B. (2022). Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome. <i>Proceedings of the Royal Society B: Biological Sciences</i>. The Royal Society. <a href=\"https://doi.org/10.1098/rspb.2021.1985\">https://doi.org/10.1098/rspb.2021.1985</a>","ama":"Kelemen RK, Elkrewi MN, Lindholm AK, Vicoso B. Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome. <i>Proceedings of the Royal Society B: Biological Sciences</i>. 2022;289(1968):20211985. doi:<a href=\"https://doi.org/10.1098/rspb.2021.1985\">10.1098/rspb.2021.1985</a>","mla":"Kelemen, Réka K., et al. “Novel Patterns of Expression and Recruitment of New Genes on the T-Haplotype, a Mouse Selfish Chromosome.” <i>Proceedings of the Royal Society B: Biological Sciences</i>, vol. 289, no. 1968, The Royal Society, 2022, p. 20211985, doi:<a href=\"https://doi.org/10.1098/rspb.2021.1985\">10.1098/rspb.2021.1985</a>.","ista":"Kelemen RK, Elkrewi MN, Lindholm AK, Vicoso B. 2022. Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome. Proceedings of the Royal Society B: Biological Sciences. 289(1968), 20211985."},"oa":1,"title":"Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome","department":[{"_id":"BeVi"}],"has_accepted_license":"1","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file_date_updated":"2022-02-21T08:17:38Z","publisher":"The Royal Society","external_id":{"pmid":["35135349"],"isi":["000752812800012"]},"acknowledgement":"This project has received funding from the European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 715257) and from the Swiss National Science Foundation (grant no. 310030_189145).\r\nWe thank Jari Garbely of the Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland, for conducting the PCR verification. Barbara\r\nKonig, Gabi Stichel and A.K.L. collected mouse tissue samples, from the field study led by R.K.K. ","project":[{"name":"Prevalence and Influence of Sexual Antagonism on Genome Evolution","_id":"250BDE62-B435-11E9-9278-68D0E5697425","grant_number":"715257","call_identifier":"H2020"}],"publication":"Proceedings of the Royal Society B: Biological Sciences","article_processing_charge":"No","scopus_import":"1","date_published":"2022-02-09T00:00:00Z","pmid":1,"license":"https://creativecommons.org/licenses/by/4.0/","date_created":"2022-02-20T23:01:31Z","_id":"10767","author":[{"full_name":"Kelemen, Réka K","last_name":"Kelemen","first_name":"Réka K","id":"48D3F8DE-F248-11E8-B48F-1D18A9856A87"},{"id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","first_name":"Marwan N","orcid":"0000-0002-5328-7231","last_name":"Elkrewi","full_name":"Elkrewi, Marwan N"},{"full_name":"Lindholm, Anna K.","first_name":"Anna K.","last_name":"Lindholm"},{"first_name":"Beatriz","orcid":"0000-0002-4579-8306","last_name":"Vicoso","full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87"}],"ec_funded":1,"abstract":[{"lang":"eng","text":"The t-haplotype of mice is a classical model for autosomal transmission distortion. A largely non-recombining variant of the proximal region of chromosome 17, it is transmitted to more than 90% of the progeny of heterozygous males through the disabling of sperm carrying a standard chromosome. While extensive genetic and functional work has shed light on individual genes involved in drive, much less is known about the evolution and function of the rest of its hundreds of genes. Here, we characterize the sequence and expression of dozens of t-specific transcripts and of their chromosome 17 homologues. Many genes showed reduced expression of the t-allele, but an equal number of genes showed increased expression of their t-copy, consistent with increased activity or a newly evolved function. Genes on the t-haplotype had a significantly higher non-synonymous substitution rate than their homologues on the standard chromosome, with several genes harbouring dN/dS ratios above 1. Finally, the t-haplotype has acquired at least two genes from other chromosomes, which show high and tissue-specific expression. These results provide a first overview of the gene content of this selfish element, and support a more dynamic evolutionary scenario than expected of a large genomic region with almost no recombination."}],"issue":"1968","intvolume":"       289","publication_status":"published","date_updated":"2023-08-02T14:26:07Z","type":"journal_article","year":"2022","doi":"10.1098/rspb.2021.1985","ddc":["570"],"month":"02","language":[{"iso":"eng"}]},{"author":[{"id":"48D3F8DE-F248-11E8-B48F-1D18A9856A87","first_name":"Réka K","orcid":"0000-0002-8489-9281","last_name":"Kelemen","full_name":"Kelemen, Réka K"},{"first_name":"H","last_name":"Rajakaruna","full_name":"Rajakaruna, H"},{"full_name":"Cockburn, IA","first_name":"IA","last_name":"Cockburn"},{"last_name":"Ganusov","first_name":"VV","full_name":"Ganusov, VV"}],"abstract":[{"lang":"eng","text":"Malaria, a disease caused by parasites of the Plasmodium genus, begins when Plasmodium-infected mosquitoes inject malaria sporozoites while searching for blood. Sporozoites migrate from the skin via blood to the liver, infect hepatocytes, and form liver stages which in mice 48 h later escape into blood and cause clinical malaria. Vaccine-induced activated or memory CD8 T cells are capable of locating and eliminating all liver stages in 48 h, thus preventing the blood-stage disease. However, the rules of how CD8 T cells are able to locate all liver stages within a relatively short time period remains poorly understood. We recently reported formation of clusters consisting of variable numbers of activated CD8 T cells around Plasmodium yoelii (Py)-infected hepatocytes. Using a combination of experimental data and mathematical models we now provide additional insights into mechanisms of formation of these clusters. First, we show that a model in which cluster formation is driven exclusively by T-cell-extrinsic factors, such as variability in “attractiveness” of different liver stages, cannot explain distribution of cluster sizes in different experimental conditions. In contrast, the model in which cluster formation is driven by the positive feedback loop (i.e., larger clusters attract more CD8 T cells) can accurately explain the available data. Second, while both Py-specific CD8 T cells and T cells of irrelevant specificity (non-specific CD8 T cells) are attracted to the clusters, we found no evidence that non-specific CD8 T cells play a role in cluster formation. Third and finally, mathematical modeling suggested that formation of clusters occurs rapidly, within few hours after adoptive transfer of CD8 T cells, thus illustrating high efficiency of CD8 T cells in locating their targets in complex peripheral organs, such as the liver. Taken together, our analysis provides novel insights into and attempts to discriminate between alternative mechanisms driving the formation of clusters of antigen-specific CD8 T cells in the liver."}],"pmid":1,"scopus_import":"1","date_published":"2019-09-20T00:00:00Z","_id":"6983","date_created":"2019-11-04T15:50:06Z","ddc":["570"],"year":"2019","doi":"10.3389/fimmu.2019.02153","language":[{"iso":"eng"}],"month":"09","intvolume":"        10","publication_status":"published","date_updated":"2023-08-30T07:18:23Z","type":"journal_article","publication_identifier":{"issn":["1664-3224"]},"quality_controlled":"1","isi":1,"citation":{"ista":"Kelemen RK, Rajakaruna H, Cockburn I, Ganusov V. 2019. Clustering of activated CD8 T cells around Malaria-infected hepatocytes is rapid and is driven by antigen-specific cells. Frontiers in Immunology. 10, 2153.","ama":"Kelemen RK, Rajakaruna H, Cockburn I, Ganusov V. Clustering of activated CD8 T cells around Malaria-infected hepatocytes is rapid and is driven by antigen-specific cells. <i>Frontiers in Immunology</i>. 2019;10. doi:<a href=\"https://doi.org/10.3389/fimmu.2019.02153\">10.3389/fimmu.2019.02153</a>","mla":"Kelemen, Réka K., et al. “Clustering of Activated CD8 T Cells around Malaria-Infected Hepatocytes Is Rapid and Is Driven by Antigen-Specific Cells.” <i>Frontiers in Immunology</i>, vol. 10, 2153, Frontiers, 2019, doi:<a href=\"https://doi.org/10.3389/fimmu.2019.02153\">10.3389/fimmu.2019.02153</a>.","apa":"Kelemen, R. K., Rajakaruna, H., Cockburn, I., &#38; Ganusov, V. (2019). Clustering of activated CD8 T cells around Malaria-infected hepatocytes is rapid and is driven by antigen-specific cells. <i>Frontiers in Immunology</i>. Frontiers. <a href=\"https://doi.org/10.3389/fimmu.2019.02153\">https://doi.org/10.3389/fimmu.2019.02153</a>","ieee":"R. K. Kelemen, H. Rajakaruna, I. Cockburn, and V. Ganusov, “Clustering of activated CD8 T cells around Malaria-infected hepatocytes is rapid and is driven by antigen-specific cells,” <i>Frontiers in Immunology</i>, vol. 10. Frontiers, 2019.","chicago":"Kelemen, Réka K, H Rajakaruna, IA Cockburn, and VV Ganusov. “Clustering of Activated CD8 T Cells around Malaria-Infected Hepatocytes Is Rapid and Is Driven by Antigen-Specific Cells.” <i>Frontiers in Immunology</i>. Frontiers, 2019. <a href=\"https://doi.org/10.3389/fimmu.2019.02153\">https://doi.org/10.3389/fimmu.2019.02153</a>.","short":"R.K. Kelemen, H. Rajakaruna, I. Cockburn, V. Ganusov, Frontiers in Immunology 10 (2019)."},"oa":1,"day":"20","article_number":"2153","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"article_type":"original","oa_version":"Published Version","volume":10,"file":[{"file_size":2083061,"creator":"dernst","file_id":"6984","content_type":"application/pdf","relation":"main_file","file_name":"2019_FrontiersImmonology_Kelemen.pdf","date_updated":"2020-07-14T12:47:46Z","date_created":"2019-11-04T15:54:00Z","access_level":"open_access","checksum":"68d1708f7aa412544159b498ef17a6b9"}],"external_id":{"isi":["000487187000001"],"pmid":["31616407"]},"article_processing_charge":"No","publication":"Frontiers in Immunology","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file_date_updated":"2020-07-14T12:47:46Z","department":[{"_id":"BeVi"}],"has_accepted_license":"1","title":"Clustering of activated CD8 T cells around Malaria-infected hepatocytes is rapid and is driven by antigen-specific cells","status":"public","publisher":"Frontiers"},{"oa_version":"Published Version","volume":208,"page":"365 - 375","article_type":"original","file":[{"access_level":"open_access","checksum":"2123845e7031a0cf043905be160f9e69","date_created":"2018-12-12T10:15:14Z","date_updated":"2020-07-14T12:46:50Z","content_type":"application/pdf","relation":"main_file","file_name":"IST-2018-1058-v1+1_365.full__1_.pdf","creator":"system","file_id":"5132","file_size":1311661}],"tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"oa":1,"citation":{"short":"R.K. Kelemen, B. Vicoso, Genetics 208 (2018) 365–375.","ieee":"R. K. Kelemen and B. Vicoso, “Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver,” <i>Genetics</i>, vol. 208, no. 1. Genetics Society of America, pp. 365–375, 2018.","chicago":"Kelemen, Réka K, and Beatriz Vicoso. “Complex History and Differentiation Patterns of the T-Haplotype, a Mouse Meiotic Driver.” <i>Genetics</i>. Genetics Society of America, 2018. <a href=\"https://doi.org/10.1534/genetics.117.300513\">https://doi.org/10.1534/genetics.117.300513</a>.","mla":"Kelemen, Réka K., and Beatriz Vicoso. “Complex History and Differentiation Patterns of the T-Haplotype, a Mouse Meiotic Driver.” <i>Genetics</i>, vol. 208, no. 1, Genetics Society of America, 2018, pp. 365–75, doi:<a href=\"https://doi.org/10.1534/genetics.117.300513\">10.1534/genetics.117.300513</a>.","ama":"Kelemen RK, Vicoso B. Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver. <i>Genetics</i>. 2018;208(1):365-375. doi:<a href=\"https://doi.org/10.1534/genetics.117.300513\">10.1534/genetics.117.300513</a>","apa":"Kelemen, R. K., &#38; Vicoso, B. (2018). Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver. <i>Genetics</i>. Genetics Society of America. <a href=\"https://doi.org/10.1534/genetics.117.300513\">https://doi.org/10.1534/genetics.117.300513</a>","ista":"Kelemen RK, Vicoso B. 2018. Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver. Genetics. 208(1), 365–375."},"day":"01","isi":1,"quality_controlled":"1","publisher":"Genetics Society of America","file_date_updated":"2020-07-14T12:46:50Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","has_accepted_license":"1","title":"Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver","department":[{"_id":"BeVi"}],"status":"public","article_processing_charge":"No","publication":"Genetics","project":[{"grant_number":"715257","call_identifier":"H2020","_id":"250BDE62-B435-11E9-9278-68D0E5697425","name":"Prevalence and Influence of Sexual Antagonism on Genome Evolution"}],"external_id":{"isi":["000419356300024"]},"publist_id":"7274","_id":"542","date_created":"2018-12-11T11:47:04Z","date_published":"2018-01-01T00:00:00Z","scopus_import":"1","related_material":{"record":[{"relation":"popular_science","status":"public","id":"5571"},{"status":"public","relation":"popular_science","id":"5572"}]},"pubrep_id":"1058","issue":"1","ec_funded":1,"abstract":[{"lang":"eng","text":"The t-haplotype, a mouse meiotic driver found on chromosome 17, has been a model for autosomal segregation distortion for close to a century, but several questions remain regarding its biology and evolutionary history. A recently published set of population genomics resources for wild mice includes several individuals heterozygous for the t-haplotype, which we use to characterize this selfish element at the genomic and transcriptomic level. Our results show that large sections of the t-haplotype have been replaced by standard homologous sequences, possibly due to occasional events of recombination, and that this complicates the inference of its history. As expected for a long genomic segment of very low recombination, the t-haplotype carries an excess of fixed nonsynonymous mutations compared to the standard chromosome. This excess is stronger for regions that have not undergone recent recombination, suggesting that occasional gene flow between the t and the standard chromosome may provide a mechanism to regenerate coding sequences that have accumulated deleterious mutations. Finally, we find that t-complex genes with altered expression largely overlap with deleted or amplified regions, and that carrying a t-haplotype alters the testis expression of genes outside of the t-complex, providing new leads into the pathways involved in the biology of this segregation distorter."}],"author":[{"first_name":"Réka K","last_name":"Kelemen","orcid":"0000-0002-8489-9281","full_name":"Kelemen, Réka K","id":"48D3F8DE-F248-11E8-B48F-1D18A9856A87"},{"id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz","first_name":"Beatriz","orcid":"0000-0002-4579-8306","last_name":"Vicoso"}],"type":"journal_article","date_updated":"2024-02-21T13:48:27Z","publication_status":"published","intvolume":"       208","language":[{"iso":"eng"}],"month":"01","ddc":["576"],"doi":"10.1534/genetics.117.300513","year":"2018"}]