---
_id: '280'
abstract:
- lang: eng
  text: Flowers have a species-specific functional life span that determines the time
    window in which pollination, fertilization and seed set can occur. The stigma
    tissue plays a key role in flower receptivity by intercepting pollen and initiating
    pollen tube growth toward the ovary. In this article, we show that a developmentally
    controlled cell death programme terminates the functional life span of stigma
    cells in Arabidopsis. We identified the leaf senescence regulator ORESARA1 (also
    known as ANAC092) and the previously uncharacterized KIRA1 (also known as ANAC074)
    as partially redundant transcription factors that modulate stigma longevity by
    controlling the expression of programmed cell death-associated genes. KIRA1 expression
    is sufficient to induce cell death and terminate floral receptivity, whereas lack
    of both KIRA1 and ORESARA1 substantially increases stigma life span. Surprisingly,
    the extension of stigma longevity is accompanied by only a moderate extension
    of flower receptivity, suggesting that additional processes participate in the
    control of the flower's receptive life span.
acknowledgement: We gratefully acknowledge funding from the Chinese Scholarship Council
  (CSC; project number 201206910025 to Z.G.), the Fonds Wetenschappelijk Onderzoek
  (FWO; project number G005112N to A.D.; fellowship number 12I7417N to Z.L.), the
  Belgian Federal Science Policy Office (BELSPO; to Y.S.), the Agency for Innovation
  by Science and Technology of Belgium (IWT; fellowship number 121110 to M.V.D.),
  the Hercules foundation (grant AUGE-09-029 to K.D.), and the ERC StG PROCELLDEATH
  (project number 639234 to M.K.N.).
article_processing_charge: No
author:
- first_name: Zhen
  full_name: Gao, Zhen
  last_name: Gao
- first_name: Anna
  full_name: Daneva, Anna
  last_name: Daneva
- first_name: Yuliya
  full_name: Salanenka, Yuliya
  id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
  last_name: Salanenka
- first_name: Matthias
  full_name: Van Durme, Matthias
  last_name: Van Durme
- first_name: Marlies
  full_name: Huysmans, Marlies
  last_name: Huysmans
- first_name: Zongcheng
  full_name: Lin, Zongcheng
  last_name: Lin
- first_name: Freya
  full_name: De Winter, Freya
  last_name: De Winter
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
- first_name: Mansour
  full_name: Karimi, Mansour
  last_name: Karimi
- first_name: Jan
  full_name: Van De Velde, Jan
  last_name: Van De Velde
- first_name: Klaas
  full_name: Vandepoele, Klaas
  last_name: Vandepoele
- first_name: Davy
  full_name: Van De Walle, Davy
  last_name: Van De Walle
- first_name: Koen
  full_name: Dewettinck, Koen
  last_name: Dewettinck
- first_name: Bart
  full_name: Lambrecht, Bart
  last_name: Lambrecht
- first_name: Moritz
  full_name: Nowack, Moritz
  last_name: Nowack
citation:
  ama: Gao Z, Daneva A, Salanenka Y, et al. KIRA1 and ORESARA1 terminate flower receptivity
    by promoting cell death in the stigma of Arabidopsis. <i>Nature Plants</i>. 2018;4(6):365-375.
    doi:<a href="https://doi.org/10.1038/s41477-018-0160-7">10.1038/s41477-018-0160-7</a>
  apa: Gao, Z., Daneva, A., Salanenka, Y., Van Durme, M., Huysmans, M., Lin, Z., …
    Nowack, M. (2018). KIRA1 and ORESARA1 terminate flower receptivity by promoting
    cell death in the stigma of Arabidopsis. <i>Nature Plants</i>. Nature Publishing
    Group. <a href="https://doi.org/10.1038/s41477-018-0160-7">https://doi.org/10.1038/s41477-018-0160-7</a>
  chicago: Gao, Zhen, Anna Daneva, Yuliya Salanenka, Matthias Van Durme, Marlies Huysmans,
    Zongcheng Lin, Freya De Winter, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity
    by Promoting Cell Death in the Stigma of Arabidopsis.” <i>Nature Plants</i>. Nature
    Publishing Group, 2018. <a href="https://doi.org/10.1038/s41477-018-0160-7">https://doi.org/10.1038/s41477-018-0160-7</a>.
  ieee: Z. Gao <i>et al.</i>, “KIRA1 and ORESARA1 terminate flower receptivity by
    promoting cell death in the stigma of Arabidopsis,” <i>Nature Plants</i>, vol.
    4, no. 6. Nature Publishing Group, pp. 365–375, 2018.
  ista: Gao Z, Daneva A, Salanenka Y, Van Durme M, Huysmans M, Lin Z, De Winter F,
    Vanneste S, Karimi M, Van De Velde J, Vandepoele K, Van De Walle D, Dewettinck
    K, Lambrecht B, Nowack M. 2018. KIRA1 and ORESARA1 terminate flower receptivity
    by promoting cell death in the stigma of Arabidopsis. Nature Plants. 4(6), 365–375.
  mla: Gao, Zhen, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity by Promoting
    Cell Death in the Stigma of Arabidopsis.” <i>Nature Plants</i>, vol. 4, no. 6,
    Nature Publishing Group, 2018, pp. 365–75, doi:<a href="https://doi.org/10.1038/s41477-018-0160-7">10.1038/s41477-018-0160-7</a>.
  short: Z. Gao, A. Daneva, Y. Salanenka, M. Van Durme, M. Huysmans, Z. Lin, F. De
    Winter, S. Vanneste, M. Karimi, J. Van De Velde, K. Vandepoele, D. Van De Walle,
    K. Dewettinck, B. Lambrecht, M. Nowack, Nature Plants 4 (2018) 365–375.
date_created: 2018-12-11T11:45:35Z
date_published: 2018-05-28T00:00:00Z
date_updated: 2023-09-13T08:24:17Z
day: '28'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0160-7
external_id:
  isi:
  - '000435571000017'
intvolume: '         4'
isi: 1
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 365 - 375
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7619'
quality_controlled: '1'
scopus_import: '1'
status: public
title: KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in
  the stigma of Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '428'
abstract:
- lang: eng
  text: The plant hormone gibberellic acid (GA) is a crucial regulator of growth and
    development. The main paradigm of GA signaling puts forward transcriptional regulation
    via the degradation of DELLA transcriptional repressors. GA has also been shown
    to regulate tropic responses by modulation of the plasma membrane incidence of
    PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular
    and molecular mechanisms by which GA redirects protein trafficking and thus regulates
    cell surface functionality. Photoconvertible reporters revealed that GA balances
    the protein traffic between the vacuole degradation route and recycling back to
    the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple
    cargos, including PIN proteins, whereas high GA levels promote their recycling
    to the plasma membrane. This GA effect requires components of the retromer complex,
    such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated
    protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates
    the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton
    is essential for the GA effect on trafficking. This GA cellular action occurs
    through DELLA proteins that regulate the MT and retromer presumably via their
    interaction partners Prefoldins (PFDs). Our study identified a branching of the
    GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating
    transcription, also target by a nontranscriptional mechanism the retromer complex
    acting at the intersection of the degradation and recycling trafficking routes.
    By this mechanism, GA can redirect receptors and transporters to the cell surface,
    thus coregulating multiple processes, including PIN-dependent auxin fluxes during
    tropic responses.
acknowledgement: "We gratefully acknowledge M. Blázquez (Instituto de Biología Molecular
  y Celular de Plantas), M. Fendrych, C. Cuesta Moliner (Institute of Science and
  Technology Austria), M. Vanstraelen, M. Nowack (Center for Plant Systems Biology,
  Ghent), C. Luschnig (Universitat fur Bodenkultur Wien, Vienna), S. Simon (Central
  European Institute of Technology, Brno), C. Sommerville (Carnegie Institution for
  Science), and Y. Gu (Penn State University) for making available the materials used
  in this study;\r\n...funding from the European Research Council (ERC) under the
  European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement
  282300.\r\nCC BY NC ND"
article_processing_charge: No
author:
- first_name: Yuliya
  full_name: Salanenka, Yuliya
  id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
  last_name: Salanenka
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Christian
  full_name: Löfke, Christian
  last_name: Löfke
- first_name: Kaori
  full_name: Tabata, Kaori
  id: 7DAAEDA4-02D0-11E9-B11A-A5A4D7DFFFD0
  last_name: Tabata
- first_name: Satoshi
  full_name: Naramoto, Satoshi
  last_name: Naramoto
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Salanenka Y, Verstraeten I, Löfke C, et al. Gibberellin DELLA signaling targets
    the retromer complex to redirect protein trafficking to the plasma membrane. <i>PNAS</i>.
    2018;115(14):3716-3721. doi:<a href="https://doi.org/10.1073/pnas.1721760115">10.1073/pnas.1721760115</a>
  apa: Salanenka, Y., Verstraeten, I., Löfke, C., Tabata, K., Naramoto, S., Glanc,
    M., &#38; Friml, J. (2018). Gibberellin DELLA signaling targets the retromer complex
    to redirect protein trafficking to the plasma membrane. <i>PNAS</i>. National
    Academy of Sciences. <a href="https://doi.org/10.1073/pnas.1721760115">https://doi.org/10.1073/pnas.1721760115</a>
  chicago: Salanenka, Yuliya, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi
    Naramoto, Matous Glanc, and Jiří Friml. “Gibberellin DELLA Signaling Targets the
    Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” <i>PNAS</i>.
    National Academy of Sciences, 2018. <a href="https://doi.org/10.1073/pnas.1721760115">https://doi.org/10.1073/pnas.1721760115</a>.
  ieee: Y. Salanenka <i>et al.</i>, “Gibberellin DELLA signaling targets the retromer
    complex to redirect protein trafficking to the plasma membrane,” <i>PNAS</i>,
    vol. 115, no. 14. National Academy of Sciences, pp. 3716–3721, 2018.
  ista: Salanenka Y, Verstraeten I, Löfke C, Tabata K, Naramoto S, Glanc M, Friml
    J. 2018. Gibberellin DELLA signaling targets the retromer complex to redirect
    protein trafficking to the plasma membrane. PNAS. 115(14), 3716–3721.
  mla: Salanenka, Yuliya, et al. “Gibberellin DELLA Signaling Targets the Retromer
    Complex to Redirect Protein Trafficking to the Plasma Membrane.” <i>PNAS</i>,
    vol. 115, no. 14, National Academy of Sciences, 2018, pp. 3716–21, doi:<a href="https://doi.org/10.1073/pnas.1721760115">10.1073/pnas.1721760115</a>.
  short: Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc,
    J. Friml, PNAS 115 (2018) 3716–3721.
date_created: 2018-12-11T11:46:25Z
date_published: 2018-04-03T00:00:00Z
date_updated: 2025-05-07T11:12:27Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1721760115
ec_funded: 1
external_id:
  isi:
  - '000429012500073'
file:
- access_level: open_access
  checksum: 1fcf7223fb8f99559cfa80bd6f24ce44
  content_type: application/pdf
  creator: dernst
  date_created: 2018-12-17T12:30:14Z
  date_updated: 2020-07-14T12:46:26Z
  file_id: '5700'
  file_name: 2018_PNAS_Salanenka.pdf
  file_size: 1924101
  relation: main_file
file_date_updated: 2020-07-14T12:46:26Z
has_accepted_license: '1'
intvolume: '       115'
isi: 1
issue: '14'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: ' 3716 - 3721'
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7395'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gibberellin DELLA signaling targets the retromer complex to redirect protein
  trafficking to the plasma membrane
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...