---
_id: '10270'
abstract:
- lang: eng
text: Plants develop new organs to adjust their bodies to dynamic changes in the
environment. How independent organs achieve anisotropic shapes and polarities
is poorly understood. To address this question, we constructed a mechano-biochemical
model for Arabidopsis root meristem growth that integrates biologically plausible
principles. Computer model simulations demonstrate how differential growth of
neighboring tissues results in the initial symmetry-breaking leading to anisotropic
root growth. Furthermore, the root growth feeds back on a polar transport network
of the growth regulator auxin. Model, predictions are in close agreement with
in vivo patterns of anisotropic growth, auxin distribution, and cell polarity,
as well as several root phenotypes caused by chemical, mechanical, or genetic
perturbations. Our study demonstrates that the combination of tissue mechanics
and polar auxin transport organizes anisotropic root growth and cell polarities
during organ outgrowth. Therefore, a mobile auxin signal transported through immobile
cells drives polarity and growth mechanics to coordinate complex organ development.
acknowledgement: 'e are grateful Richard Smith, Anne-Lise Routier, Crisanto Gutierrez
and Juergen Kleine-Vehn for providing critical comments on the manuscript. Funding:
This work was supported by the Programa de Atraccion de Talento 2017 (Comunidad
de Madrid, 2017-T1/BIO-5654 to KW), Severo Ochoa (SO) Programme for Centres of Excellence
in R&D from the Agencia Estatal de Investigacion of Spain (grant SEV-2016–0672 (2017–2021)
to KW via the CBGP). In the frame of SEV-2016–0672 funding MM is supported with
a postdoctoral contract. KW was supported by Programa Estatal de Generacion del
Conocimiento y Fortalecimiento Cientıfico y Tecnologico del Sistema de I + D + I
2019 (PGC2018-093387-A-I00) from MICIU (to KW). MG is recipient of an IST Interdisciplinary
Project (IC1022IPC03).'
article_number: '72132'
article_processing_charge: Yes
article_type: original
author:
- first_name: Marco
full_name: Marconi, Marco
last_name: Marconi
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Krzysztof
full_name: Wabnik, Krzysztof
last_name: Wabnik
citation:
ama: Marconi M, Gallemi M, Benková E, Wabnik K. A coupled mechano-biochemical model
for cell polarity guided anisotropic root growth. eLife. 2021;10. doi:10.7554/elife.72132
apa: Marconi, M., Gallemi, M., Benková, E., & Wabnik, K. (2021). A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth. ELife. eLife Sciences
Publications. https://doi.org/10.7554/elife.72132
chicago: Marconi, Marco, Marçal Gallemi, Eva Benková, and Krzysztof Wabnik. “A Coupled
Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” ELife.
eLife Sciences Publications, 2021. https://doi.org/10.7554/elife.72132.
ieee: M. Marconi, M. Gallemi, E. Benková, and K. Wabnik, “A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth,” eLife, vol. 10.
eLife Sciences Publications, 2021.
ista: Marconi M, Gallemi M, Benková E, Wabnik K. 2021. A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth. eLife. 10, 72132.
mla: Marconi, Marco, et al. “A Coupled Mechano-Biochemical Model for Cell Polarity
Guided Anisotropic Root Growth.” ELife, vol. 10, 72132, eLife Sciences
Publications, 2021, doi:10.7554/elife.72132.
short: M. Marconi, M. Gallemi, E. Benková, K. Wabnik, ELife 10 (2021).
date_created: 2021-11-11T10:05:18Z
date_published: 2021-11-01T00:00:00Z
date_updated: 2023-08-14T11:49:23Z
day: '01'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.7554/elife.72132
external_id:
isi:
- '000734671200001'
pmid:
- '34723798'
file:
- access_level: open_access
checksum: fad13c509b53bb7a2bef9c946a7ca60a
content_type: application/pdf
creator: dernst
date_created: 2022-05-13T09:00:29Z
date_updated: 2022-05-13T09:00:29Z
file_id: '11372'
file_name: 2021_eLife_Marconi.pdf
file_size: 14137503
relation: main_file
success: 1
file_date_updated: 2022-05-13T09:00:29Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: A coupled mechano-biochemical model for cell polarity guided anisotropic root
growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...
---
_id: '9986'
abstract:
- lang: eng
text: Size control is a fundamental question in biology, showing incremental complexity
in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a
vital growth regulator with central importance for differential growth control.
Our results indicate that auxin-reliant growth programs affect the molecular complexity
of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent
induction and repression of growth coincide with reduced and enhanced molecular
complexity of xyloglucans, respectively. In agreement with a proposed function
in growth control, genetic interference with xyloglucan side decorations distinctly
modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent
growth programs have a spatially defined effect on xyloglucan’s molecular structure,
which in turn affects cell wall mechanics and specifies differential, gravitropic
hypocotyl growth.
acknowledgement: "We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and
Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for
access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical
reading.\r\n"
article_number: '9222'
article_processing_charge: Yes
article_type: original
author:
- first_name: Silvia Melina
full_name: Velasquez, Silvia Melina
last_name: Velasquez
- first_name: Xiaoyuan
full_name: Guo, Xiaoyuan
last_name: Guo
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Bibek
full_name: Aryal, Bibek
last_name: Aryal
- first_name: Peter
full_name: Venhuizen, Peter
last_name: Venhuizen
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
- first_name: Kai Alexander
full_name: Dünser, Kai Alexander
last_name: Dünser
- first_name: Martin
full_name: Darino, Martin
last_name: Darino
- first_name: Aleš
full_name: Pӗnčík, Aleš
last_name: Pӗnčík
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Maria
full_name: Kalyna, Maria
last_name: Kalyna
- first_name: Gregory
full_name: Mouille, Gregory
last_name: Mouille
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Rishikesh P.
full_name: Bhalerao, Rishikesh P.
last_name: Bhalerao
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine-Vehn
citation:
ama: Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent
differential tissue expansion in plants. International Journal of Molecular
Sciences. 2021;22(17). doi:10.3390/ijms22179222
apa: Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E.,
… Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential
tissue expansion in plants. International Journal of Molecular Sciences.
MDPI. https://doi.org/10.3390/ijms22179222
chicago: Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter
Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines
Auxin-Dependent Differential Tissue Expansion in Plants.” International Journal
of Molecular Sciences. MDPI, 2021. https://doi.org/10.3390/ijms22179222.
ieee: S. M. Velasquez et al., “Xyloglucan remodeling defines auxin-dependent
differential tissue expansion in plants,” International Journal of Molecular
Sciences, vol. 22, no. 17. MDPI, 2021.
ista: Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA,
Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec
J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential
tissue expansion in plants. International Journal of Molecular Sciences. 22(17),
9222.
mla: Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent
Differential Tissue Expansion in Plants.” International Journal of Molecular
Sciences, vol. 22, no. 17, 9222, MDPI, 2021, doi:10.3390/ijms22179222.
short: S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A.
Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P.
Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences
22 (2021).
date_created: 2021-09-05T22:01:24Z
date_published: 2021-08-26T00:00:00Z
date_updated: 2023-10-31T19:29:38Z
day: '26'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.3390/ijms22179222
external_id:
isi:
- '000694347100001'
pmid:
- '34502129'
file:
- access_level: open_access
checksum: 6b7055cf89f1b7ed8594c3fdf56f000b
content_type: application/pdf
creator: cchlebak
date_created: 2021-09-06T12:50:19Z
date_updated: 2021-09-07T09:04:53Z
file_id: '9988'
file_name: 2021_IntJMolecularSciences_Velasquez.pdf
file_size: 2162247
relation: main_file
file_date_updated: 2021-09-07T09:04:53Z
has_accepted_license: '1'
intvolume: ' 22'
isi: 1
issue: '17'
keyword:
- auxin
- growth
- cell wall
- xyloglucans
- hypocotyls
- gravitropism
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
issn:
- 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Xyloglucan remodeling defines auxin-dependent differential tissue expansion
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...
---
_id: '7805'
abstract:
- lang: eng
text: Plants as non-mobile organisms constantly integrate varying environmental
signals to flexibly adapt their growth and development. Local fluctuations in
water and nutrient availability, sudden changes in temperature or other abiotic
and biotic stresses can trigger changes in the growth of plant organs. Multiple
mutually interconnected hormonal signaling cascades act as essential endogenous
translators of these exogenous signals in the adaptive responses of plants. Although
the molecular backbones of hormone transduction pathways have been identified,
the mechanisms underlying their interactions are largely unknown. Here, using
genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk
component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots
is strictly dependent on both of these hormonal pathways. We show that SYAC1 is
a regulator of secretory pathway, whose enhanced activity interferes with deposition
of cell wall components and can fine-tune organ growth and sensitivity to soil
pathogens.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical
reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing
published material, Lesia Rodriguez Solovey for technical assistance. This work
was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B.,
the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme
(Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the
Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship
No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through
resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB
benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).
article_number: '2170'
article_processing_charge: No
article_type: original
author:
- first_name: Andrej
full_name: Hurny, Andrej
id: 4DC4AF46-F248-11E8-B48F-1D18A9856A87
last_name: Hurny
orcid: 0000-0003-3638-1426
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Jerome
full_name: Duclercq, Jerome
last_name: Duclercq
- first_name: Ladislav
full_name: Dokládal, Ladislav
last_name: Dokládal
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Hana
full_name: Semeradova, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semeradova
- first_name: Thomas
full_name: Rauter, Thomas
id: A0385D1A-9376-11EA-A47D-9862C5E3AB22
last_name: Rauter
- first_name: Irene
full_name: Stenzel, Irene
last_name: Stenzel
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Freia
full_name: Benade, Freia
last_name: Benade
- first_name: Rishikesh
full_name: Bhalearo, Rishikesh
last_name: Bhalearo
- first_name: Eva
full_name: Sýkorová, Eva
last_name: Sýkorová
- first_name: András
full_name: Gorzsás, András
last_name: Gorzsás
- first_name: Julien
full_name: Sechet, Julien
last_name: Sechet
- first_name: Gregory
full_name: Mouille, Gregory
last_name: Mouille
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jutta
full_name: Ludwig-Müller, Jutta
last_name: Ludwig-Müller
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1
positively regulates growth and attenuates soil pathogen resistance. Nature
Communications. 2020;11. doi:10.1038/s41467-020-15895-5
apa: Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L.,
… Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-020-15895-5
chicago: Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome
Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin
and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.”
Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-15895-5.
ieee: A. Hurny et al., “Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance,” Nature Communications,
vol. 11. Springer Nature, 2020.
ista: Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos
López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo
R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller
J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.
mla: Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates
Growth and Attenuates Soil Pathogen Resistance.” Nature Communications,
vol. 11, 2170, Springer Nature, 2020, doi:10.1038/s41467-020-15895-5.
short: A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C.
Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau,
F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann,
G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).
date_created: 2020-05-10T22:00:48Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-08-21T06:21:56Z
day: '01'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.1038/s41467-020-15895-5
ec_funded: 1
external_id:
isi:
- '000531425900012'
pmid:
- '32358503'
file:
- access_level: open_access
checksum: 2cba327c9e9416d75cb96be54b0fb441
content_type: application/pdf
creator: dernst
date_created: 2020-10-06T07:47:53Z
date_updated: 2020-10-06T07:47:53Z
file_id: '8614'
file_name: 2020_NatureComm_Hurny.pdf
file_size: 4743576
relation: main_file
success: 1
file_date_updated: 2020-10-06T07:47:53Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates
soil pathogen resistance
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '7350'
abstract:
- lang: eng
text: The ability to sense environmental temperature and to coordinate growth and
development accordingly, is critical to the reproductive success of plants. Flowering
time is regulated at the level of gene expression by a complex network of factors
that integrate environmental and developmental cues. One of the main players,
involved in modulating flowering time in response to changes in ambient temperature
is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative
splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative.
While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ
has the opposite effect on flowering. Here we show that the cyclin-dependent kinase
G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative
splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature
range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced
while FLM-δ is increased in a temperature dependent manner and these changes are
associated with an early flowering phenotype in the cdkg2 mutant lines. In addition,
we found that transcript variants retaining the full FLM intron 1 are sequestered
in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1.
Our results provide evidence that temperature and CDKs regulate the alternative
splicing of FLM, contributing to flowering time definition.
article_number: '1680'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: John H.
full_name: Doonan, John H.
last_name: Doonan
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
citation:
ama: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative
splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. Frontiers
in Plant Science. 2020;10. doi:10.3389/fpls.2019.01680
apa: Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., & Cavallari, N. (2020).
Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent
kinase G2. Frontiers in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2019.01680
chicago: Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola
Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated
by Cyclin-Dependent Kinase G2.” Frontiers in Plant Science. Frontiers Media,
2020. https://doi.org/10.3389/fpls.2019.01680.
ieee: C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive
alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
G2,” Frontiers in Plant Science, vol. 10. Frontiers Media, 2020.
ista: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive
alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
G2. Frontiers in Plant Science. 10, 1680.
mla: Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING
LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” Frontiers in Plant Science,
vol. 10, 1680, Frontiers Media, 2020, doi:10.3389/fpls.2019.01680.
short: C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in
Plant Science 10 (2020).
date_created: 2020-01-22T15:23:57Z
date_published: 2020-01-22T00:00:00Z
date_updated: 2023-08-17T14:21:45Z
day: '22'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2019.01680
external_id:
isi:
- '000511376000001'
file:
- access_level: open_access
checksum: d1f92e60a713fbd15097ce895e5c7ccb
content_type: application/pdf
creator: dernst
date_created: 2020-01-27T09:07:02Z
date_updated: 2020-07-14T12:47:56Z
file_id: '7366'
file_name: 2020_FrontiersPlantScience_Nibau.pdf
file_size: 1951438
relation: main_file
file_date_updated: 2020-07-14T12:47:56Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
issn:
- 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by
cyclin-dependent kinase G2
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2020'
...
---
_id: '6897'
abstract:
- lang: eng
text: The apical hook is a transiently formed structure that plays a protective
role when the germinating seedling penetrates through the soil towards the surface.
Crucial for proper bending is the local auxin maxima, which defines the concave
(inner) side of the hook curvature. As no sign of asymmetric auxin distribution
has been reported in embryonic hypocotyls prior to hook formation, the question
of how auxin asymmetry is established in the early phases of seedling germination
remains largely unanswered. Here, we analyzed the auxin distribution and expression
of PIN auxin efflux carriers from early phases of germination, and show that bending
of the root in response to gravity is the crucial initial cue that governs the
hypocotyl bending required for apical hook formation. Importantly, polar auxin
transport machinery is established gradually after germination starts as a result
of tight root-hypocotyl interaction and a proper balance between abscisic acid
and gibberellins.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Jiri Friml and Phillip Brewer for inspiring discussion
and for help in preparing the manuscript. This research was supported by the Scientific
Service Units (SSU) of IST-Austria through resources provided by the Bioimaging
Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by
grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development
Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738)."
article_number: dev175919
article_processing_charge: No
article_type: original
author:
- first_name: Qiang
full_name: Zhu, Qiang
id: 40A4B9E6-F248-11E8-B48F-1D18A9856A87
last_name: Zhu
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Petra
full_name: Žádníková, Petra
last_name: Žádníková
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity
response module guides differential growth determining both root bending and apical
hook formation in Arabidopsis. Development. 2019;146(17). doi:10.1242/dev.175919
apa: Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., & Benková,
E. (2019). Root gravity response module guides differential growth determining
both root bending and apical hook formation in Arabidopsis. Development.
The Company of Biologists. https://doi.org/10.1242/dev.175919
chicago: Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad,
and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining
Both Root Bending and Apical Hook Formation in Arabidopsis.” Development.
The Company of Biologists, 2019. https://doi.org/10.1242/dev.175919.
ieee: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková,
“Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis,” Development, vol. 146,
no. 17. The Company of Biologists, 2019.
ista: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root
gravity response module guides differential growth determining both root bending
and apical hook formation in Arabidopsis. Development. 146(17), dev175919.
mla: Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth
Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” Development,
vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:10.1242/dev.175919.
short: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development
146 (2019).
date_created: 2019-09-22T22:00:36Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2025-05-07T11:10:55Z
day: '12'
department:
- _id: EvBe
doi: 10.1242/dev.175919
ec_funded: 1
external_id:
isi:
- '000486297400011'
pmid:
- '31391194'
intvolume: ' 146'
isi: 1
issue: '17'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.175919
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Development
publication_identifier:
eissn:
- '14779129'
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 146
year: '2019'
...
---
_id: '1258'
abstract:
- lang: eng
text: When plants grow in close proximity basic resources such as light can become
limiting. Under such conditions plants respond to anticipate and/or adapt to the
light shortage, a process known as the shade avoidance syndrome (SAS). Following
genetic screening using a shade-responsive luciferase reporter line (PHYB:LUC),
we identified DRACULA2 (DRA2), which encodes an Arabidopsis homolog of mammalian
nucleoporin 98, a component of the nuclear pore complex (NPC). DRA2, together
with other nucleoporins, participates positively in the control of the hypocotyl
elongation response to plant proximity, a role that can be considered dependent
on the nucleocytoplasmic transport of macromolecules (i.e. is transport dependent).
In addition, our results reveal a specific role for DRA2 in controlling shade-induced
gene expression. We suggest that this novel regulatory role of DRA2 is transport
independent and that it might rely on its dynamic localization within and outside
of the NPC. These results provide mechanistic insights in to how SAS responses
are rapidly established by light conditions. They also indicate that nucleoporins
have an active role in plant signaling.
acknowledgement: M.G. received an FPI fellowship from the Spanish Ministerio de Economía
y Competitividad (MINECO). A.G. and A.F.-A. received FPU fellowships from the Spanish
Ministerio de Educación. S.P. received an FI fellowship from the Agència de Gestió
D'ajuts Universitaris i de Recerca (AGAUR - Generalitat de Catalunya). C.T. received
a Marie Curie IEF postdoctoral contract funded by the European Commission. I.R.-V.
received initially an FPI fellowship from the Spanish MINECO and later a Beatriu
de Pinós contract from AGAUR. Our research is supported by grants from the Spanish
MINECO-FEDER [BIO2008-00169, BIO2011-23489 and BIO2014-59895-P] and Generalitat
de Catalunya [2011-SGR447 and Xarba] to J.F.M.-G., and Generalitat Valenciana [PROMETEO/2009/112,
PROMETEOII/2014/006] to M.R.P. and J.L.M. We acknowledge the support of the Spanish
MINECO for the ‘Centro de Excelencia Severo Ochoa 2016-2019’ [award SEV-2015-0533].
We thank the CRAG greenhouse service for plant care; Chus Burillo for technical
help; Sergi Portolés and Carles Rentero for assistance with mutagenesis; Mark Estelle
(UCSD, USA) for providing sar1-4, sar3-1 and sar3-3 seeds; Juanjo López-Moya (CRAG,
Barcelona; 35S:HcPro plasmid) and Dolors Ludevid (CRAG; C307 plasmid) for providing
DNA plasmids; and Manuel Rodríguez-Concepción (CRAG) and Miguel Blázquez (IBMCP,
Valencia, Spain) for comments on the manuscript.
author:
- first_name: Marcal
full_name: Gallemi Rovira, Marcal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi Rovira
- first_name: Anahit
full_name: Galstyan, Anahit
last_name: Galstyan
- first_name: Sandi
full_name: Paulišić, Sandi
last_name: Paulišić
- first_name: Christiane
full_name: Then, Christiane
last_name: Then
- first_name: Almudena
full_name: Ferrández Ayela, Almudena
last_name: Ferrández Ayela
- first_name: Laura
full_name: Lorenzo Orts, Laura
last_name: Lorenzo Orts
- first_name: Irma
full_name: Roig Villanova, Irma
last_name: Roig Villanova
- first_name: Xuewen
full_name: Wang, Xuewen
last_name: Wang
- first_name: José
full_name: Micol, José
last_name: Micol
- first_name: Maria
full_name: Ponce, Maria
last_name: Ponce
- first_name: Paul
full_name: Devlin, Paul
last_name: Devlin
- first_name: Jaime
full_name: Martínez García, Jaime
last_name: Martínez García
citation:
ama: Gallemi M, Galstyan A, Paulišić S, et al. DRACULA2 is a dynamic nucleoporin
with a role in regulating the shade avoidance syndrome in Arabidopsis. Development.
2016;143(9):1623-1631. doi:10.1242/dev.130211
apa: Gallemi, M., Galstyan, A., Paulišić, S., Then, C., Ferrández Ayela, A., Lorenzo
Orts, L., … Martínez García, J. (2016). DRACULA2 is a dynamic nucleoporin with
a role in regulating the shade avoidance syndrome in Arabidopsis. Development.
Company of Biologists. https://doi.org/10.1242/dev.130211
chicago: Gallemi, Marçal, Anahit Galstyan, Sandi Paulišić, Christiane Then, Almudena
Ferrández Ayela, Laura Lorenzo Orts, Irma Roig Villanova, et al. “DRACULA2 Is
a Dynamic Nucleoporin with a Role in Regulating the Shade Avoidance Syndrome in
Arabidopsis.” Development. Company of Biologists, 2016. https://doi.org/10.1242/dev.130211.
ieee: M. Gallemi et al., “DRACULA2 is a dynamic nucleoporin with a role in
regulating the shade avoidance syndrome in Arabidopsis,” Development, vol.
143, no. 9. Company of Biologists, pp. 1623–1631, 2016.
ista: Gallemi M, Galstyan A, Paulišić S, Then C, Ferrández Ayela A, Lorenzo Orts
L, Roig Villanova I, Wang X, Micol J, Ponce M, Devlin P, Martínez García J. 2016.
DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance
syndrome in Arabidopsis. Development. 143(9), 1623–1631.
mla: Gallemi, Marçal, et al. “DRACULA2 Is a Dynamic Nucleoporin with a Role in Regulating
the Shade Avoidance Syndrome in Arabidopsis.” Development, vol. 143, no.
9, Company of Biologists, 2016, pp. 1623–31, doi:10.1242/dev.130211.
short: M. Gallemi, A. Galstyan, S. Paulišić, C. Then, A. Ferrández Ayela, L. Lorenzo
Orts, I. Roig Villanova, X. Wang, J. Micol, M. Ponce, P. Devlin, J. Martínez García,
Development 143 (2016) 1623–1631.
date_created: 2018-12-11T11:50:59Z
date_published: 2016-05-03T00:00:00Z
date_updated: 2021-01-12T06:49:27Z
day: '03'
department:
- _id: EvBe
doi: 10.1242/dev.130211
intvolume: ' 143'
issue: '9'
language:
- iso: eng
month: '05'
oa_version: None
page: 1623 - 1631
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '6068'
quality_controlled: '1'
scopus_import: 1
status: public
title: DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance
syndrome in Arabidopsis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 143
year: '2016'
...
---
_id: '1265'
abstract:
- lang: eng
text: Extracellular matrices (ECMs) are central to the advent of multicellular life,
and their mechanical propertiesare modulated by and impinge on intracellular signaling
pathways that regulate vital cellular functions. High spatial-resolution mapping
of mechanical properties in live cells is, however, extremely challenging. Thus,
our understanding of how signaling pathways process physiological signals to generate
appropriate mechanical responses is limited. We introduce fluorescence emission-Brillouin
scattering imaging (FBi), a method for the parallel and all-optical measurements
of mechanical properties and fluorescence at the submicrometer scale in living
organisms. Using FBi, we showed thatchanges in cellular hydrostatic pressure and
cytoplasm viscoelasticity modulate the mechanical signatures of plant ECMs. We
further established that the measured "stiffness" of plant ECMs is symmetrically
patternedin hypocotyl cells undergoing directional growth. Finally, application
of this method to Arabidopsis thaliana with photoreceptor mutants revealed that
red and far-red light signals are essential modulators of ECM viscoelasticity.
By mapping the viscoelastic signatures of a complex ECM, we provide proof of principlefor
the organism-wide applicability of FBi for measuring the mechanical outputs of
intracellular signaling pathways. As such, our work has implications for investigations
of mechanosignaling pathways and developmental biology.
article_number: rs5
author:
- first_name: Kareem
full_name: Elsayad, Kareem
last_name: Elsayad
- first_name: Stephanie
full_name: Werner, Stephanie
last_name: Werner
- first_name: Marcal
full_name: Gallemi Rovira, Marcal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi Rovira
- first_name: Jixiang
full_name: Kong, Jixiang
last_name: Kong
- first_name: Edmundo
full_name: Guajardo, Edmundo
last_name: Guajardo
- first_name: Lijuan
full_name: Zhang, Lijuan
last_name: Zhang
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Thomas
full_name: Greb, Thomas
last_name: Greb
- first_name: Youssef
full_name: Belkhadir, Youssef
last_name: Belkhadir
citation:
ama: Elsayad K, Werner S, Gallemi M, et al. Mapping the subcellular mechanical properties
of live cells in tissues with fluorescence emission-Brillouin imaging. Science
Signaling. 2016;9(435). doi:10.1126/scisignal.aaf6326
apa: Elsayad, K., Werner, S., Gallemi, M., Kong, J., Guajardo, E., Zhang, L., …
Belkhadir, Y. (2016). Mapping the subcellular mechanical properties of live cells
in tissues with fluorescence emission-Brillouin imaging. Science Signaling.
American Association for the Advancement of Science. https://doi.org/10.1126/scisignal.aaf6326
chicago: Elsayad, Kareem, Stephanie Werner, Marçal Gallemi, Jixiang Kong, Edmundo
Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, and Youssef Belkhadir. “Mapping
the Subcellular Mechanical Properties of Live Cells in Tissues with Fluorescence
Emission-Brillouin Imaging.” Science Signaling. American Association for
the Advancement of Science, 2016. https://doi.org/10.1126/scisignal.aaf6326.
ieee: K. Elsayad et al., “Mapping the subcellular mechanical properties of
live cells in tissues with fluorescence emission-Brillouin imaging,” Science
Signaling, vol. 9, no. 435. American Association for the Advancement of Science,
2016.
ista: Elsayad K, Werner S, Gallemi M, Kong J, Guajardo E, Zhang L, Jaillais Y, Greb
T, Belkhadir Y. 2016. Mapping the subcellular mechanical properties of live cells
in tissues with fluorescence emission-Brillouin imaging. Science Signaling. 9(435),
rs5.
mla: Elsayad, Kareem, et al. “Mapping the Subcellular Mechanical Properties of Live
Cells in Tissues with Fluorescence Emission-Brillouin Imaging.” Science Signaling,
vol. 9, no. 435, rs5, American Association for the Advancement of Science, 2016,
doi:10.1126/scisignal.aaf6326.
short: K. Elsayad, S. Werner, M. Gallemi, J. Kong, E. Guajardo, L. Zhang, Y. Jaillais,
T. Greb, Y. Belkhadir, Science Signaling 9 (2016).
date_created: 2018-12-11T11:51:02Z
date_published: 2016-07-05T00:00:00Z
date_updated: 2021-01-12T06:49:29Z
day: '05'
department:
- _id: EvBe
doi: 10.1126/scisignal.aaf6326
intvolume: ' 9'
issue: '435'
language:
- iso: eng
month: '07'
oa_version: None
publication: Science Signaling
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '6057'
quality_controlled: '1'
scopus_import: 1
status: public
title: Mapping the subcellular mechanical properties of live cells in tissues with
fluorescence emission-Brillouin imaging
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2016'
...