[{"acknowledgement":"We apologize to all the authors whose scientific work could not be cited and discussed because of space restrictions. We thank Dr. Inge Verstraeten (ISTAustria) and Dr. Juan Carlos Montesinos-Lopez (ETH Zürich) for helpful suggestions. This work was supported by the DOC Fellowship Programme of the Austrian Academy of Sciences (25008) to C.A.","intvolume":"        13","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/cshperspect.a039941"}],"title":"Auxin-regulated lateral root organogenesis","publication":"Cold Spring Harbor Perspectives in Biology","article_processing_charge":"No","language":[{"iso":"eng"}],"year":"2021","date_updated":"2023-09-27T06:44:06Z","citation":{"ista":"Cavallari N, Artner C, Benková E. 2021. Auxin-regulated lateral root organogenesis. Cold Spring Harbor Perspectives in Biology. 13(7), a039941.","chicago":"Cavallari, Nicola, Christina Artner, and Eva Benková. “Auxin-Regulated Lateral Root Organogenesis.” <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press, 2021. <a href=\"https://doi.org/10.1101/cshperspect.a039941\">https://doi.org/10.1101/cshperspect.a039941</a>.","apa":"Cavallari, N., Artner, C., &#38; Benková, E. (2021). Auxin-regulated lateral root organogenesis. <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/cshperspect.a039941\">https://doi.org/10.1101/cshperspect.a039941</a>","short":"N. Cavallari, C. Artner, E. Benková, Cold Spring Harbor Perspectives in Biology 13 (2021).","ieee":"N. Cavallari, C. Artner, and E. Benková, “Auxin-regulated lateral root organogenesis,” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 13, no. 7. Cold Spring Harbor Laboratory Press, 2021.","mla":"Cavallari, Nicola, et al. “Auxin-Regulated Lateral Root Organogenesis.” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 13, no. 7, a039941, Cold Spring Harbor Laboratory Press, 2021, doi:<a href=\"https://doi.org/10.1101/cshperspect.a039941\">10.1101/cshperspect.a039941</a>.","ama":"Cavallari N, Artner C, Benková E. Auxin-regulated lateral root organogenesis. <i>Cold Spring Harbor Perspectives in Biology</i>. 2021;13(7). doi:<a href=\"https://doi.org/10.1101/cshperspect.a039941\">10.1101/cshperspect.a039941</a>"},"pmid":1,"type":"journal_article","article_number":"a039941","month":"07","date_published":"2021-07-01T00:00:00Z","scopus_import":"1","article_type":"original","doi":"10.1101/cshperspect.a039941","oa_version":"Published Version","isi":1,"day":"01","department":[{"_id":"EvBe"}],"quality_controlled":"1","oa":1,"issue":"7","publisher":"Cold Spring Harbor Laboratory Press","status":"public","date_created":"2021-03-01T10:08:32Z","abstract":[{"text":"Plant fitness is largely dependent on the root, the underground organ, which, besides its anchoring function, supplies the plant body with water and all nutrients necessary for growth and development. To exploit the soil effectively, roots must constantly integrate environmental signals and react through adjustment of growth and development. Important components of the root management strategy involve a rapid modulation of the root growth kinetics and growth direction, as well as an increase of the root system radius through formation of lateral roots (LRs). At the molecular level, such a fascinating growth and developmental flexibility of root organ requires regulatory networks that guarantee stability of the developmental program but also allows integration of various environmental inputs. The plant hormone auxin is one of the principal endogenous regulators of root system architecture by controlling primary root growth and formation of LR. In this review, we discuss recent progress in understanding molecular networks where auxin is one of the main players shaping the root system and acting as mediator between endogenous cues and environmental factors.","lang":"eng"}],"_id":"9212","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","external_id":{"isi":["000692069100001"],"pmid":["33558367"]},"author":[{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"id":"45DF286A-F248-11E8-B48F-1D18A9856A87","last_name":"Artner","first_name":"Christina","full_name":"Artner, Christina"},{"full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"project":[{"_id":"2685A872-B435-11E9-9278-68D0E5697425","name":"Hormonal regulation of plant adaptive responses to environmental signals"}],"volume":13,"publication_identifier":{"issn":["1943-0264"]}},{"article_type":"original","doi":"10.3389/fpls.2019.01680","file_date_updated":"2020-07-14T12:47:56Z","oa_version":"Published Version","article_number":"1680","has_accepted_license":"1","citation":{"mla":"Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>, vol. 10, 1680, Frontiers Media, 2020, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>.","ieee":"C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2,” <i>Frontiers in Plant Science</i>, vol. 10. Frontiers Media, 2020.","ama":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. 2020;10. doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>","chicago":"Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2020. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>.","ista":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. Frontiers in Plant Science. 10, 1680.","short":"C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in Plant Science 10 (2020).","apa":"Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., &#38; Cavallari, N. (2020). Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>"},"date_updated":"2023-08-17T14:21:45Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"scopus_import":"1","month":"01","date_published":"2020-01-22T00:00:00Z","ddc":["580"],"article_processing_charge":"No","publication":"Frontiers in Plant Science","language":[{"iso":"eng"}],"year":"2020","intvolume":"        10","title":"Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2","external_id":{"isi":["000511376000001"]},"author":[{"last_name":"Nibau","first_name":"Candida","full_name":"Nibau, Candida"},{"full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","last_name":"Gallemi","orcid":"0000-0003-4675-6893","first_name":"Marçal"},{"full_name":"Dadarou, Despoina","first_name":"Despoina","last_name":"Dadarou"},{"first_name":"John H.","last_name":"Doonan","full_name":"Doonan, John H."},{"full_name":"Cavallari, Nicola","first_name":"Nicola","last_name":"Cavallari","id":"457160E6-F248-11E8-B48F-1D18A9856A87"}],"volume":10,"publication_identifier":{"issn":["1664-462X"]},"_id":"7350","abstract":[{"lang":"eng","text":"The ability to sense environmental temperature and to coordinate growth and development accordingly, is critical to the reproductive success of plants. Flowering time is regulated at the level of gene expression by a complex network of factors that integrate environmental and developmental cues. One of the main players, involved in modulating flowering time in response to changes in ambient temperature is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative. While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ has the opposite effect on flowering. Here we show that the cyclin-dependent kinase G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced while FLM-δ is increased in a temperature dependent manner and these changes are associated with an early flowering phenotype in the cdkg2 mutant lines. In addition, we found that transcript variants retaining the full FLM intron 1 are sequestered in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1. Our results provide evidence that temperature and CDKs regulate the alternative splicing of FLM, contributing to flowering time definition."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","publisher":"Frontiers Media","file":[{"date_updated":"2020-07-14T12:47:56Z","creator":"dernst","content_type":"application/pdf","file_size":1951438,"file_id":"7366","relation":"main_file","access_level":"open_access","file_name":"2020_FrontiersPlantScience_Nibau.pdf","date_created":"2020-01-27T09:07:02Z","checksum":"d1f92e60a713fbd15097ce895e5c7ccb"}],"status":"public","date_created":"2020-01-22T15:23:57Z","department":[{"_id":"EvBe"}],"isi":1,"day":"22","oa":1,"quality_controlled":"1"},{"article_number":"2170","type":"journal_article","pmid":1,"has_accepted_license":"1","date_updated":"2023-08-21T06:21:56Z","citation":{"apa":"Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L., … Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>","short":"A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C. Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau, F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann, G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).","ista":"Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.","chicago":"Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>.","ama":"Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>","ieee":"A. Hurny <i>et al.</i>, “Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>, vol. 11, 2170, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>."},"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"month":"05","date_published":"2020-05-01T00:00:00Z","ec_funded":1,"doi":"10.1038/s41467-020-15895-5","article_type":"original","file_date_updated":"2020-10-06T07:47:53Z","oa_version":"Published Version","acknowledgement":"We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing published material, Lesia Rodriguez Solovey for technical assistance. This work was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B., the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","title":"Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance","intvolume":"        11","article_processing_charge":"No","publication":"Nature Communications","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2020","language":[{"iso":"eng"}],"_id":"7805","abstract":[{"lang":"eng","text":"Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens."}],"publication_status":"published","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"pmid":["32358503"],"isi":["000531425900012"]},"author":[{"full_name":"Hurny, Andrej","last_name":"Hurny","orcid":"0000-0003-3638-1426","first_name":"Andrej","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Cuesta, Candela","first_name":"Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös","full_name":"Ötvös, Krisztina"},{"full_name":"Duclercq, Jerome","last_name":"Duclercq","first_name":"Jerome"},{"full_name":"Dokládal, Ladislav","last_name":"Dokládal","first_name":"Ladislav"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","last_name":"Montesinos López","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C"},{"id":"460C6802-F248-11E8-B48F-1D18A9856A87","first_name":"Marçal","orcid":"0000-0003-4675-6893","last_name":"Gallemi","full_name":"Gallemi, Marçal"},{"full_name":"Semeradova, Hana","last_name":"Semeradova","first_name":"Hana","id":"42FE702E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rauter, Thomas","id":"A0385D1A-9376-11EA-A47D-9862C5E3AB22","first_name":"Thomas","last_name":"Rauter"},{"full_name":"Stenzel, Irene","first_name":"Irene","last_name":"Stenzel"},{"full_name":"Persiau, Geert","last_name":"Persiau","first_name":"Geert"},{"first_name":"Freia","last_name":"Benade","full_name":"Benade, Freia"},{"last_name":"Bhalearo","first_name":"Rishikesh","full_name":"Bhalearo, Rishikesh"},{"last_name":"Sýkorová","first_name":"Eva","full_name":"Sýkorová, Eva"},{"last_name":"Gorzsás","first_name":"András","full_name":"Gorzsás, András"},{"first_name":"Julien","last_name":"Sechet","full_name":"Sechet, Julien"},{"full_name":"Mouille, Gregory","first_name":"Gregory","last_name":"Mouille"},{"last_name":"Heilmann","first_name":"Ingo","full_name":"Heilmann, Ingo"},{"full_name":"De Jaeger, Geert","first_name":"Geert","last_name":"De Jaeger"},{"first_name":"Jutta","last_name":"Ludwig-Müller","full_name":"Ludwig-Müller, Jutta"},{"first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"}],"publication_identifier":{"eissn":["20411723"]},"volume":11,"project":[{"grant_number":"I 1774-B16","call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development"},{"call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"}],"department":[{"_id":"EvBe"}],"day":"01","isi":1,"oa":1,"quality_controlled":"1","file":[{"date_updated":"2020-10-06T07:47:53Z","creator":"dernst","content_type":"application/pdf","file_size":4743576,"file_id":"8614","relation":"main_file","access_level":"open_access","file_name":"2020_NatureComm_Hurny.pdf","date_created":"2020-10-06T07:47:53Z","checksum":"2cba327c9e9416d75cb96be54b0fb441","success":1}],"publisher":"Springer Nature","date_created":"2020-05-10T22:00:48Z","status":"public"},{"oa_version":"Published Version","file_date_updated":"2020-09-10T08:05:19Z","doi":"10.1038/s41467-020-17949-0","article_type":"original","ec_funded":1,"date_published":"2020-08-27T00:00:00Z","month":"08","ddc":["580"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"scopus_import":"1","citation":{"ama":"Kubiasova K, Montesinos López JC, Šamajová O, et al. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-17949-0\">10.1038/s41467-020-17949-0</a>","ieee":"K. Kubiasova <i>et al.</i>, “Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Kubiasova, Karolina, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” <i>Nature Communications</i>, vol. 11, 4285, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-17949-0\">10.1038/s41467-020-17949-0</a>.","apa":"Kubiasova, K., Montesinos López, J. C., Šamajová, O., Nisler, J., Mik, V., Semerádová, H., … Spíchal, L. (2020). Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17949-0\">https://doi.org/10.1038/s41467-020-17949-0</a>","short":"K. Kubiasova, J.C. Montesinos López, O. Šamajová, J. Nisler, V. Mik, H. Semerádová, L. Plíhalová, O. Novák, P. Marhavý, N. Cavallari, D. Zalabák, K. Berka, K. Doležal, P. Galuszka, J. Šamaj, M. Strnad, E. Benková, O. Plíhal, L. Spíchal, Nature Communications 11 (2020).","ista":"Kubiasova K, Montesinos López JC, Šamajová O, Nisler J, Mik V, Semerádová H, Plíhalová L, Novák O, Marhavý P, Cavallari N, Zalabák D, Berka K, Doležal K, Galuszka P, Šamaj J, Strnad M, Benková E, Plíhal O, Spíchal L. 2020. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 11, 4285.","chicago":"Kubiasova, Karolina, Juan C Montesinos López, Olga Šamajová, Jaroslav Nisler, Václav Mik, Hana Semerádová, Lucie Plíhalová, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17949-0\">https://doi.org/10.1038/s41467-020-17949-0</a>."},"has_accepted_license":"1","date_updated":"2023-08-22T09:09:06Z","type":"journal_article","pmid":1,"article_number":"4285","language":[{"iso":"eng"}],"year":"2020","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication":"Nature Communications","article_processing_charge":"No","intvolume":"        11","title":"Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum","acknowledgement":"This paper is dedicated to deceased P. Galuszka for his support and contribution to the project. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF) and by Centre of the Region Haná (CRH), Palacký University. We thank Lucia Hlusková, Zuzana Pěkná and Martin Hönig for technical assistance, and Fernando Aniento, Rashed Abualia and Andrej Hurný for sharing material. The work was supported from ERDF project “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827), from Czech Science Foundation via projects 16-04184S (O.P., K.K. and K.D.), 18-23972Y (D.Z., K.K.), 17-21122S (K.B.), Erasmus+ (K.K.), Endowment Fund of Palacký University (K.K.) and EMBO Long-Term Fellowship, ALTF number 710-2016 (J.C.M.); People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. [291734] (N.C.); DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology, Austria (H.S.).","project":[{"grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"},{"grant_number":"24746","_id":"261821BC-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of the cytokinin regulated endomembrane trafficking to coordinate plant organogenesis."},{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","_id":"253E54C8-B435-11E9-9278-68D0E5697425","grant_number":"ALTF710-2016"}],"volume":11,"publication_identifier":{"eissn":["20411723"]},"external_id":{"pmid":["32855390"],"isi":["000567931000002"]},"author":[{"first_name":"Karolina","last_name":"Kubiasova","orcid":"0000-0001-5630-9419","id":"946011F4-3E71-11EA-860B-C7A73DDC885E","full_name":"Kubiasova, Karolina"},{"full_name":"Montesinos López, Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","last_name":"Montesinos López","orcid":"0000-0001-9179-6099"},{"first_name":"Olga","last_name":"Šamajová","full_name":"Šamajová, Olga"},{"full_name":"Nisler, Jaroslav","last_name":"Nisler","first_name":"Jaroslav"},{"last_name":"Mik","first_name":"Václav","full_name":"Mik, Václav"},{"first_name":"Hana","last_name":"Semeradova","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","full_name":"Semeradova, Hana"},{"full_name":"Plíhalová, Lucie","first_name":"Lucie","last_name":"Plíhalová"},{"full_name":"Novák, Ondřej","last_name":"Novák","first_name":"Ondřej"},{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","last_name":"Marhavý","first_name":"Peter","full_name":"Marhavý, Peter"},{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"last_name":"Zalabák","first_name":"David","full_name":"Zalabák, David"},{"first_name":"Karel","last_name":"Berka","full_name":"Berka, Karel"},{"full_name":"Doležal, Karel","last_name":"Doležal","first_name":"Karel"},{"last_name":"Galuszka","first_name":"Petr","full_name":"Galuszka, Petr"},{"full_name":"Šamaj, Jozef","last_name":"Šamaj","first_name":"Jozef"},{"first_name":"Miroslav","last_name":"Strnad","full_name":"Strnad, Miroslav"},{"full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Plíhal","first_name":"Ondřej","full_name":"Plíhal, Ondřej"},{"full_name":"Spíchal, Lukáš","last_name":"Spíchal","first_name":"Lukáš"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","abstract":[{"lang":"eng","text":"Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER."}],"_id":"8336","status":"public","date_created":"2020-09-06T22:01:12Z","publisher":"Springer Nature","file":[{"date_created":"2020-09-10T08:05:19Z","checksum":"7494b7665b3d2bf2d8edb13e4f12b92d","success":1,"access_level":"open_access","file_name":"2020_NatureComm_Kubiasova.pdf","file_id":"8357","relation":"main_file","date_updated":"2020-09-10T08:05:19Z","creator":"dernst","content_type":"application/pdf","file_size":3455704}],"quality_controlled":"1","oa":1,"isi":1,"day":"27","department":[{"_id":"EvBe"}]},{"year":"2020","language":[{"iso":"eng"}],"publication":"Frontiers in Plant Science","article_processing_charge":"No","intvolume":"        11","title":"A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis","acknowledgement":"CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1). NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna.","file_date_updated":"2020-12-09T09:14:19Z","oa_version":"Published Version","doi":"10.3389/fpls.2020.586870","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"scopus_import":"1","month":"11","date_published":"2020-11-10T00:00:00Z","ddc":["580"],"article_number":"586870","date_updated":"2023-08-24T10:50:00Z","citation":{"ama":"Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. <i>Frontiers in Plant Science</i>. 2020;11. doi:<a href=\"https://doi.org/10.3389/fpls.2020.586870\">10.3389/fpls.2020.586870</a>","ieee":"C. Nibau <i>et al.</i>, “A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,” <i>Frontiers in Plant Science</i>, vol. 11. Frontiers, 2020.","mla":"Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.” <i>Frontiers in Plant Science</i>, vol. 11, 586870, Frontiers, 2020, doi:<a href=\"https://doi.org/10.3389/fpls.2020.586870\">10.3389/fpls.2020.586870</a>.","apa":"Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N., Cavallari, N., &#38; Doonan, J. H. (2020). A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. <i>Frontiers in Plant Science</i>. Frontiers. <a href=\"https://doi.org/10.3389/fpls.2020.586870\">https://doi.org/10.3389/fpls.2020.586870</a>","short":"C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N. Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).","ista":"Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers in Plant Science. 11, 586870.","chicago":"Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.” <i>Frontiers in Plant Science</i>. Frontiers, 2020. <a href=\"https://doi.org/10.3389/fpls.2020.586870\">https://doi.org/10.3389/fpls.2020.586870</a>."},"has_accepted_license":"1","type":"journal_article","status":"public","date_created":"2020-12-06T23:01:14Z","file":[{"file_name":"2020_Frontiers_Nibau.pdf","access_level":"open_access","checksum":"1c0ee6ce9950aa665d6a5cc64aa6b752","date_created":"2020-12-09T09:14:19Z","success":1,"creator":"dernst","date_updated":"2020-12-09T09:14:19Z","file_size":1833244,"content_type":"application/pdf","relation":"main_file","file_id":"8929"}],"publisher":"Frontiers","oa":1,"quality_controlled":"1","department":[{"_id":"EvBe"}],"isi":1,"day":"10","volume":11,"publication_identifier":{"eissn":["1664-462X"]},"author":[{"full_name":"Nibau, Candida","last_name":"Nibau","first_name":"Candida"},{"full_name":"Dadarou, Despoina","last_name":"Dadarou","first_name":"Despoina"},{"full_name":"Kargios, Nestoras","last_name":"Kargios","first_name":"Nestoras"},{"full_name":"Mallioura, Areti","first_name":"Areti","last_name":"Mallioura"},{"first_name":"Narcis","last_name":"Fernandez-Fuentes","full_name":"Fernandez-Fuentes, Narcis"},{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"first_name":"John H.","last_name":"Doonan","full_name":"Doonan, John H."}],"external_id":{"isi":["000591637000001"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","_id":"8924","abstract":[{"text":"Maintaining fertility in a fluctuating environment is key to the reproductive success of flowering plants. Meiosis and pollen formation are particularly sensitive to changes in growing conditions, especially temperature. We have previously identified cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent meiosis and this may involve the regulation of alternative splicing (AS), including of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain which may be involved in co-factor interaction. In leaves, both isoforms have distinct temperature-dependent functions on target mRNA processing, but their role in pollen development is unknown. In the present study, we characterize the role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the long (L) form is necessary and sufficient to rescue the fertility defects of the cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of the ATP binding pocket of the kinase indicates that kinase activity is necessary for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore, we show that there is partial functional overlap between CDKG1 and its paralog CDKG2 that could in part be explained by overlapping gene expression.","lang":"eng"}]},{"publist_id":"7616","doi":"10.1038/s41598-018-27080-2","file_date_updated":"2020-07-14T12:45:49Z","oa_version":"Published Version","article_number":"8754","type":"journal_article","date_updated":"2023-09-13T08:59:27Z","citation":{"mla":"Ceinos, Rosa Maria, et al. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>, vol. 8, no. 1, 8754, Nature Publishing Group, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>.","ieee":"R. M. Ceinos <i>et al.</i>, “Mutations in blind cavefish target the light regulated circadian clock gene period 2,” <i>Scientific Reports</i>, vol. 8, no. 1. Nature Publishing Group, 2018.","ama":"Ceinos RM, Frigato E, Pagano C, et al. Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>","chicago":"Ceinos, Rosa Maria, Elena Frigato, Cristina Pagano, Nadine Frohlich, Pietro Negrini, Nicola Cavallari, Daniela Vallone, Silvia Fuselli, Cristiano Bertolucci, and Nicholas S Foulkes. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>.","ista":"Ceinos RM, Frigato E, Pagano C, Frohlich N, Negrini P, Cavallari N, Vallone D, Fuselli S, Bertolucci C, Foulkes NS. 2018. Mutations in blind cavefish target the light regulated circadian clock gene period 2. Scientific Reports. 8(1), 8754.","short":"R.M. Ceinos, E. Frigato, C. Pagano, N. Frohlich, P. Negrini, N. Cavallari, D. Vallone, S. Fuselli, C. Bertolucci, N.S. Foulkes, Scientific Reports 8 (2018).","apa":"Ceinos, R. M., Frigato, E., Pagano, C., Frohlich, N., Negrini, P., Cavallari, N., … Foulkes, N. S. (2018). Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>"},"has_accepted_license":"1","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"date_published":"2018-06-08T00:00:00Z","month":"06","article_processing_charge":"No","publication":"Scientific Reports","year":"2018","language":[{"iso":"eng"}],"title":"Mutations in blind cavefish target the light regulated circadian clock gene period 2","intvolume":"         8","author":[{"full_name":"Ceinos, Rosa Maria","first_name":"Rosa Maria","last_name":"Ceinos"},{"last_name":"Frigato","first_name":"Elena","full_name":"Frigato, Elena"},{"full_name":"Pagano, Cristina","last_name":"Pagano","first_name":"Cristina"},{"first_name":"Nadine","last_name":"Frohlich","full_name":"Frohlich, Nadine"},{"full_name":"Negrini, Pietro","last_name":"Negrini","first_name":"Pietro"},{"first_name":"Nicola","last_name":"Cavallari","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"last_name":"Vallone","first_name":"Daniela","full_name":"Vallone, Daniela"},{"last_name":"Fuselli","first_name":"Silvia","full_name":"Fuselli, Silvia"},{"first_name":"Cristiano","last_name":"Bertolucci","full_name":"Bertolucci, Cristiano"},{"first_name":"Nicholas S","last_name":"Foulkes","full_name":"Foulkes, Nicholas S"}],"external_id":{"isi":["000434640800008"]},"volume":8,"_id":"283","abstract":[{"lang":"eng","text":"Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors."}],"publication_status":"published","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"access_level":"open_access","file_name":"2018_ScientificReports_Ceinos.pdf","date_created":"2018-12-17T13:04:46Z","checksum":"9c3942d772f84f3df032ffde0ed9a8ea","creator":"dernst","date_updated":"2020-07-14T12:45:49Z","content_type":"application/pdf","file_size":1855324,"file_id":"5707","relation":"main_file"}],"publisher":"Nature Publishing Group","date_created":"2018-12-11T11:45:36Z","status":"public","department":[{"_id":"EvBe"}],"day":"08","isi":1,"oa":1,"issue":"1","quality_controlled":"1"},{"publication":"The Plant Journal","article_processing_charge":"No","year":"2018","language":[{"iso":"eng"}],"acknowledgement":"CN, DD and JHD were funded by the BBSRC (grant number BB/M009459/1). NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna. AB and AF were supported by the Austrian Science Fund (FWF) [DK W1207; SFB RNAreg F43-P10]","intvolume":"        94","title":"The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A","publist_id":"7426","doi":"10.1111/tpj.13914","file_date_updated":"2020-07-14T12:46:22Z","oa_version":"Published Version","has_accepted_license":"1","citation":{"ama":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. 2018;94(6):1010-1022. doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>","ieee":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, and J. Doonan, “The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A,” <i>The Plant Journal</i>, vol. 94, no. 6. Wiley, pp. 1010–1022, 2018.","mla":"Cavallari, Nicola, et al. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>, vol. 94, no. 6, Wiley, 2018, pp. 1010–22, doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>.","apa":"Cavallari, N., Nibau, C., Fuchs, A., Dadarou, D., Barta, A., &#38; Doonan, J. (2018). The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>","short":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, J. Doonan, The Plant Journal 94 (2018) 1010–1022.","ista":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. 2018. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 94(6), 1010–1022.","chicago":"Cavallari, Nicola, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea Barta, and John Doonan. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>."},"date_updated":"2023-09-19T10:07:08Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"scopus_import":"1","date_published":"2018-06-01T00:00:00Z","month":"06","ddc":["580"],"publisher":"Wiley","file":[{"file_id":"5934","relation":"main_file","creator":"dernst","date_updated":"2020-07-14T12:46:22Z","content_type":"application/pdf","file_size":1543354,"date_created":"2019-02-06T11:40:54Z","checksum":"d9d3ad3215ac0e581731443fca312266","access_level":"open_access","file_name":"2018_PlantJourn_Cavallari.pdf"}],"page":"1010 - 1022","status":"public","date_created":"2018-12-11T11:46:17Z","department":[{"_id":"EvBe"}],"isi":1,"day":"01","issue":"6","oa":1,"quality_controlled":"1","author":[{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"},{"last_name":"Nibau","first_name":"Candida","full_name":"Nibau, Candida"},{"full_name":"Fuchs, Armin","first_name":"Armin","last_name":"Fuchs"},{"full_name":"Dadarou, Despoina","last_name":"Dadarou","first_name":"Despoina"},{"full_name":"Barta, Andrea","first_name":"Andrea","last_name":"Barta"},{"full_name":"Doonan, John","first_name":"John","last_name":"Doonan"}],"external_id":{"isi":["000434365500008"]},"volume":94,"_id":"403","abstract":[{"text":"The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin-dependent Kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature-dependent manner. We found that this process is partly dependent on both the Cyclin-dependent Kinase G2 (CDKG2) and the interacting co-factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature-dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A. This article is protected by copyright. All rights reserved.","lang":"eng"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_status":"published"}]