---
_id: '8002'
abstract:
- lang: eng
text: Wound healing in plant tissues, consisting of rigid cell wall-encapsulated
cells, represents a considerable challenge and occurs through largely unknown
mechanisms distinct from those in animals. Owing to their inability to migrate,
plant cells rely on targeted cell division and expansion to regenerate wounds.
Strict coordination of these wound-induced responses is essential to ensure efficient,
spatially restricted wound healing. Single-cell tracking by live imaging allowed
us to gain mechanistic insight into the wound perception and coordination of wound
responses after laser-based wounding in Arabidopsis root. We revealed a crucial
contribution of the collapse of damaged cells in wound perception and detected
an auxin increase specific to cells immediately adjacent to the wound. This localized
auxin increase balances wound-induced cell expansion and restorative division
rates in a dose-dependent manner, leading to tumorous overproliferation when the
canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure
changes together also spatially define the activation of key components of regeneration,
such as the transcription regulator ERF115. Our observations suggest that the
wound signaling involves the sensing of collapse of damaged cells and a local
auxin signaling activation to coordinate the downstream transcriptional responses
in the immediate wound vicinity.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_number: '202003346'
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
Wounding-induced changes in cellular pressure and localized auxin signalling spatially
coordinate restorative divisions in roots. Proceedings of the National Academy
of Sciences. 2020;117(26). doi:10.1073/pnas.2003346117
apa: Hörmayer, L., Montesinos López, J. C., Marhavá, P., Benková, E., Yoshida, S.,
& Friml, J. (2020). Wounding-induced changes in cellular pressure and localized
auxin signalling spatially coordinate restorative divisions in roots. Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences. https://doi.org/10.1073/pnas.2003346117
chicago: Hörmayer, Lukas, Juan C Montesinos López, Petra Marhavá, Eva Benková, Saiko
Yoshida, and Jiří Friml. “Wounding-Induced Changes in Cellular Pressure and Localized
Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences, 2020. https://doi.org/10.1073/pnas.2003346117.
ieee: L. Hörmayer, J. C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, and
J. Friml, “Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots,” Proceedings of the National
Academy of Sciences, vol. 117, no. 26. Proceedings of the National Academy
of Sciences, 2020.
ista: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
2020. Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots. Proceedings of the National
Academy of Sciences. 117(26), 202003346.
mla: Hörmayer, Lukas, et al. “Wounding-Induced Changes in Cellular Pressure and
Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.”
Proceedings of the National Academy of Sciences, vol. 117, no. 26, 202003346,
Proceedings of the National Academy of Sciences, 2020, doi:10.1073/pnas.2003346117.
short: L. Hörmayer, J.C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, J.
Friml, Proceedings of the National Academy of Sciences 117 (2020).
date_created: 2020-06-22T13:33:52Z
date_published: 2020-06-30T00:00:00Z
date_updated: 2024-03-25T23:30:06Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1073/pnas.2003346117
ec_funded: 1
external_id:
isi:
- '000565729700033'
pmid:
- '32541049'
file:
- access_level: open_access
checksum: 908b09437680181de9990915f2113aca
content_type: application/pdf
creator: dernst
date_created: 2020-06-23T11:30:53Z
date_updated: 2020-07-14T12:48:07Z
file_id: '8009'
file_name: 2020_PNAS_Hoermayer.pdf
file_size: 2407102
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
has_accepted_license: '1'
intvolume: ' 117'
isi: 1
issue: '26'
language:
- iso: eng
month: '06'
oa: 1
oa_version: None
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/how-wounded-plants-coordinate-their-healing/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 117
year: '2020'
...
---
_id: '7619'
abstract:
- lang: eng
text: Cell polarity is a fundamental feature of all multicellular organisms. In
plants, prominent cell polarity markers are PIN auxin transporters crucial for
plant development. To identify novel components involved in cell polarity establishment
and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2
Arabidopsis plants, which ectopically express predominantly basally localized
PIN1 in the root epidermal cells leading to agravitropic root growth. From the
screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which
restored gravitropic root growth and caused PIN1-HA polarity switch from basal
to apical side of root epidermal cells. Complementation experiments established
the repp12 causative mutation as an amino acid substitution in Aminophospholipid
ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation.
ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric
auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple
mutants confirmed a crucial role of ALA proteins in regulating plant development
and in PIN trafficking and polarity. Genetic and physical interaction studies
revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together,
our results identified ALA3 flippase as an important interactor and regulator
of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development.
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
article_type: original
author:
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Vendula
full_name: Pukyšová, Vendula
last_name: Pukyšová
- first_name: Adrià Sans
full_name: Sánchez, Adrià Sans
last_name: Sánchez
- first_name: Vivek Kumar
full_name: Raxwal, Vivek Kumar
last_name: Raxwal
- first_name: Christian S.
full_name: Hardtke, Christian S.
last_name: Hardtke
- first_name: Tomasz
full_name: Nodzynski, Tomasz
last_name: Nodzynski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with
ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin
transporters. The Plant Cell. 2020;32(5):1644-1664. doi:10.1105/tpc.19.00869
apa: Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey,
L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange
factors to regulate the trafficking and polarity of PIN auxin transporters. The
Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.19.00869
chicago: Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang,
Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate
with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN
Auxin Transporters.” The Plant Cell. American Society of Plant Biologists,
2020. https://doi.org/10.1105/tpc.19.00869.
ieee: X. Zhang et al., “Arabidopsis flippases cooperate with ARF GTPase exchange
factors to regulate the trafficking and polarity of PIN auxin transporters,” The
Plant Cell, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664,
2020.
ista: Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka
M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020.
Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the
trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.
mla: Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange
Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” The
Plant Cell, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp.
1644–64, doi:10.1105/tpc.19.00869.
short: X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey,
M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski,
J. Friml, The Plant Cell 32 (2020) 1644–1664.
date_created: 2020-03-28T07:39:22Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-09-05T12:21:06Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.19.00869
ec_funded: 1
external_id:
isi:
- '000545741500030'
pmid:
- '32193204'
intvolume: ' 32'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1105/tpc.19.00869
month: '05'
oa: 1
oa_version: Published Version
page: 1644-1664
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate
the trafficking and polarity of PIN auxin transporters
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 32
year: '2020'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-25T23:30:06Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
- '000466843000015'
pmid:
- '31051107'
file:
- access_level: open_access
checksum: 4ceba04a96a74f5092ec3ce2c579a0c7
content_type: application/pdf
creator: dernst
date_created: 2019-05-13T06:12:45Z
date_updated: 2020-07-14T12:47:28Z
file_id: '6411'
file_name: 2019_Cell_Marhava.pdf
file_size: 10272032
relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 957-969.e13
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '1492'
abstract:
- lang: eng
text: To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated
cells with a preserved prolif eration capacity. The root pericycle represents
a unique tissue with conditional meristematic activity, and its tight control
determines initiation of lateral organs. Here we show that the meristematic activity
of the pericycle is constrained by the interaction with the adjacent endodermis.
Release of these restraints by elimination of endo dermal cells by single-cell
ablation triggers the pericycle to re-enter the cell cycle. We found that endodermis
removal substitutes for the phytohormone auxin-dependent initiation of the pericycle
meristematic activity. However, auxin is indispensable to steer the cell division
plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally
distinct role for auxin during lateral root initiation. In the endodermis, auxin
releases constraints arising from cell-to-cell interactions that compromise the
pericycle meristematic activity, whereas, in the pericycle, auxin defines the
orientation of the cell division plane to initiate lateral roots.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: 'This work was supported by a European Research Council Starting
Inde-pendent Research grant (ERC-2007-Stg-207362-HCPO to J.D.), Research Foundation-Flanders
(G033711N to A.A.), and the Austrian Science Fund (FWF01_I1774S to E.B.). P.M. is
indebted to the Federation of European Biochemical Sciences for a Long-Term Fellowship. '
author:
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Anas
full_name: Abuzeineh, Anas
last_name: Abuzeineh
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Joop
full_name: Vermeer, Joop
last_name: Vermeer
- first_name: Jérôme
full_name: Duclercq, Jérôme
last_name: Duclercq
- first_name: Hana
full_name: Rakusova, Hana
last_name: Rakusova
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Niko
full_name: Geldner, Niko
last_name: Geldner
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Marhavý P, Montesinos López JC, Abuzeineh A, et al. Targeted cell elimination
reveals an auxin-guided biphasic mode of lateral root initiation. Genes and
Development. 2016;30(4):471-483. doi:10.1101/gad.276964.115
apa: Marhavý, P., Montesinos López, J. C., Abuzeineh, A., Van Damme, D., Vermeer,
J., Duclercq, J., … Benková, E. (2016). Targeted cell elimination reveals an auxin-guided
biphasic mode of lateral root initiation. Genes and Development. Cold Spring
Harbor Laboratory Press. https://doi.org/10.1101/gad.276964.115
chicago: Marhavý, Peter, Juan C Montesinos López, Anas Abuzeineh, Daniël Van Damme,
Joop Vermeer, Jérôme Duclercq, Hana Rakusova, et al. “Targeted Cell Elimination
Reveals an Auxin-Guided Biphasic Mode of Lateral Root Initiation.” Genes and
Development. Cold Spring Harbor Laboratory Press, 2016. https://doi.org/10.1101/gad.276964.115.
ieee: P. Marhavý et al., “Targeted cell elimination reveals an auxin-guided
biphasic mode of lateral root initiation,” Genes and Development, vol.
30, no. 4. Cold Spring Harbor Laboratory Press, pp. 471–483, 2016.
ista: Marhavý P, Montesinos López JC, Abuzeineh A, Van Damme D, Vermeer J, Duclercq
J, Rakusova H, Marhavá P, Friml J, Geldner N, Benková E. 2016. Targeted cell elimination
reveals an auxin-guided biphasic mode of lateral root initiation. Genes and Development.
30(4), 471–483.
mla: Marhavý, Peter, et al. “Targeted Cell Elimination Reveals an Auxin-Guided Biphasic
Mode of Lateral Root Initiation.” Genes and Development, vol. 30, no. 4,
Cold Spring Harbor Laboratory Press, 2016, pp. 471–83, doi:10.1101/gad.276964.115.
short: P. Marhavý, J.C. Montesinos López, A. Abuzeineh, D. Van Damme, J. Vermeer,
J. Duclercq, H. Rakusova, P. Marhavá, J. Friml, N. Geldner, E. Benková, Genes
and Development 30 (2016) 471–483.
date_created: 2018-12-11T11:52:20Z
date_published: 2016-03-01T00:00:00Z
date_updated: 2021-01-12T06:51:08Z
day: '01'
ddc:
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department:
- _id: EvBe
doi: 10.1101/gad.276964.115
external_id:
pmid:
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creator: kschuh
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page: 471 - 483
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publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '5691'
quality_controlled: '1'
scopus_import: 1
status: public
title: Targeted cell elimination reveals an auxin-guided biphasic mode of lateral
root initiation
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
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volume: 30
year: '2016'
...
---
_id: '1893'
abstract:
- lang: eng
text: Phosphatidylinositol (PtdIns) is a structural phospholipid that can be phosphorylated
into various lipid signaling molecules, designated polyphosphoinositides (PPIs).
The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol
is performed by a set of organelle-specific kinases and phosphatases, and the
characteristic head groups make these molecules ideal for regulating biological
processes in time and space. In yeast and mammals, PtdIns3P and PtdIns(3,5)P2
play crucial roles in trafficking toward the lytic compartments, whereas the role
in plants is not yet fully understood. Here we identified the role of a land plant-specific
subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during
vacuolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize
to the tonoplast along with PtdIns3P, the presumable product of their activity.
In SAC gain- and loss-of-function mutants, the levels of PtdIns monophosphates
and bisphosphates were changed, with opposite effects on the morphology of storage
and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover,
multiple sac knockout mutants had an increased number of smaller storage and lytic
vacuoles, whereas extralarge vacuoles were observed in the overexpression lines,
correlating with various growth and developmental defects. The fragmented vacuolar
phenotype of sac mutants could be mimicked by treating wild-type seedlings with
PtdIns(3,5)P2, corroborating that this PPI is important for vacuole morphology.
Taken together, these results provide evidence that PPIs, together with their
metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar
morphology and function in plants.
acknowledgement: This work was supported by grants from the Research Foundation-Flanders
(Odysseus).
author:
- first_name: Petra
full_name: Nováková, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Nováková
- first_name: Sibylle
full_name: Hirsch, Sibylle
last_name: Hirsch
- first_name: Elena
full_name: Feraru, Elena
last_name: Feraru
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Ringo
full_name: Van Wijk, Ringo
last_name: Van Wijk
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Mareike
full_name: Heilmann, Mareike
last_name: Heilmann
- first_name: Jennifer
full_name: Lerche, Jennifer
last_name: Lerche
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Mugurel
full_name: Feraru, Mugurel
last_name: Feraru
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Marc
full_name: Van Montagu, Marc
last_name: Van Montagu
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Teun
full_name: Munnik, Teun
last_name: Munnik
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hirsch S, Feraru E, et al. SAC phosphoinositide phosphatases at
the tonoplast mediate vacuolar function in Arabidopsis. PNAS. 2014;111(7):2818-2823.
doi:10.1073/pnas.1324264111
apa: Marhavá, P., Hirsch, S., Feraru, E., Tejos, R., Van Wijk, R., Viaene, T., …
Friml, J. (2014). SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar
function in Arabidopsis. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1324264111
chicago: Marhavá, Petra, Sibylle Hirsch, Elena Feraru, Ricardo Tejos, Ringo Van
Wijk, Tom Viaene, Mareike Heilmann, et al. “SAC Phosphoinositide Phosphatases
at the Tonoplast Mediate Vacuolar Function in Arabidopsis.” PNAS. National
Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1324264111.
ieee: P. Marhavá et al., “SAC phosphoinositide phosphatases at the tonoplast
mediate vacuolar function in Arabidopsis,” PNAS, vol. 111, no. 7. National
Academy of Sciences, pp. 2818–2823, 2014.
ista: Marhavá P, Hirsch S, Feraru E, Tejos R, Van Wijk R, Viaene T, Heilmann M,
Lerche J, De Rycke R, Feraru M, Grones P, Van Montagu M, Heilmann I, Munnik T,
Friml J. 2014. SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar
function in Arabidopsis. PNAS. 111(7), 2818–2823.
mla: Marhavá, Petra, et al. “SAC Phosphoinositide Phosphatases at the Tonoplast
Mediate Vacuolar Function in Arabidopsis.” PNAS, vol. 111, no. 7, National
Academy of Sciences, 2014, pp. 2818–23, doi:10.1073/pnas.1324264111.
short: P. Marhavá, S. Hirsch, E. Feraru, R. Tejos, R. Van Wijk, T. Viaene, M. Heilmann,
J. Lerche, R. De Rycke, M. Feraru, P. Grones, M. Van Montagu, I. Heilmann, T.
Munnik, J. Friml, PNAS 111 (2014) 2818–2823.
date_created: 2018-12-11T11:54:34Z
date_published: 2014-02-18T00:00:00Z
date_updated: 2021-01-12T06:53:53Z
day: '18'
department:
- _id: JiFr
doi: 10.1073/pnas.1324264111
ec_funded: 1
intvolume: ' 111'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932866/
month: '02'
oa: 1
oa_version: Submitted Version
page: 2818 - 2823
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '5202'
scopus_import: 1
status: public
title: SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function
in Arabidopsis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 111
year: '2014'
...
---
_id: '1402'
abstract:
- lang: eng
text: Phosphatidylinositol (Ptdlns) is a structural phospholipid that can be phosphorylated
into various lipid signaling molecules, designated polyphosphoinositides (PPIs).
The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol
is performed by a set of organelle-specific kinases and phosphatases, and the
characteristic head groups make these molecules ideal for regulating biological
processes in time and space. In yeast and mammals, Ptdlns3P and Ptdlns(3,5)P2
play crucial roles in trafficking toward the lytic compartments, whereas the role
in plants is not yet fully understood. Here we identified the role of a land plant-specific
subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during
vauolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize
to the tonoplast along with Ptdlns3P, the presumable product of their activity.
in SAC gain- and loss-of-function mutants, the levels of Ptdlns monophosphates
and bisphosphates were changed, with opposite effects on the morphology of storage
and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover,
multiple sac knockout mutants had an increased number of smaller storage and lytic
vacuoles, whereas extralarge vacuoles were observed in the overexpression lines,
correlating with various growth and developmental defects. The fragmented vacuolar
phenotype of sac mutants could be mimicked by treating wild-type seedlings with
Ptdlns(3,5)P2, corroborating that this PPI is important for vacuole morphology.
Taken together, these results provide evidence that PPIs, together with their
metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar
morphology and function in plants.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
citation:
ama: Marhavá P. Molecular mechanisms of patterning and subcellular trafficking in
Arabidopsis thaliana. 2014.
apa: Marhavá, P. (2014). Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria.
chicago: Marhavá, Petra. “Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2014.
ieee: P. Marhavá, “Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2014.
ista: Marhavá P. 2014. Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria.
mla: Marhavá, Petra. Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana. Institute of Science and Technology Austria, 2014.
short: P. Marhavá, Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2014.
date_created: 2018-12-11T11:51:49Z
date_published: 2014-12-01T00:00:00Z
date_updated: 2023-09-07T11:39:38Z
day: '01'
degree_awarded: PhD
department:
- _id: JiFr
language:
- iso: eng
month: '12'
oa_version: None
page: '90'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '5805'
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis
thaliana
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2014'
...