---
_id: '14082'
abstract:
- lang: eng
  text: Epithelial barrier function is commonly analyzed using transepithelial electrical
    resistance, which measures ion flux across a monolayer, or by adding traceable
    macromolecules and monitoring their passage across the monolayer. Although these
    methods measure changes in global barrier function, they lack the sensitivity
    needed to detect local or transient barrier breaches, and they do not reveal the
    location of barrier leaks. Therefore, we previously developed a method that we
    named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which
    overcomes these limitations, allowing for detection of local tight junction leaks
    with high spatiotemporal resolution. Here, we present expanded applications for
    ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier
    restoration and actin accumulation following laser injury. ZnUMBA can also be
    effectively utilized in developing zebrafish embryos as well as cultured monolayers
    of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful
    and flexible method that, with minimal optimization, can be applied to multiple
    systems to measure dynamic changes in barrier function with spatiotemporal precision.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "The authors thank their respective lab members for feedback and
  helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria
  for their support.\r\nThis work was supported by the National Institutes of Health
  [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan
  Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for
  Biomedical Engineering Research from the Nakatani Foundation for Advancement of
  Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the
  European Research Council [advanced grant 742573 to C.-P.H.]. "
article_number: jcs260668
article_processing_charge: No
article_type: original
author:
- first_name: Tomohito
  full_name: Higashi, Tomohito
  last_name: Higashi
- first_name: Rachel E.
  full_name: Stephenson, Rachel E.
  last_name: Stephenson
- first_name: Cornelia
  full_name: Schwayer, Cornelia
  id: 3436488C-F248-11E8-B48F-1D18A9856A87
  last_name: Schwayer
  orcid: 0000-0001-5130-2226
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
- first_name: Atsuko Y.
  full_name: Higashi, Atsuko Y.
  last_name: Higashi
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Hideki
  full_name: Chiba, Hideki
  last_name: Chiba
- first_name: Ann L.
  full_name: Miller, Ann L.
  last_name: Miller
citation:
  ama: Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method
    to detect local barrier breaches. <i>Journal of Cell Science</i>. 2023;136(15).
    doi:<a href="https://doi.org/10.1242/jcs.260668">10.1242/jcs.260668</a>
  apa: Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg,
    C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local
    barrier breaches. <i>Journal of Cell Science</i>. The Company of Biologists. <a
    href="https://doi.org/10.1242/jcs.260668">https://doi.org/10.1242/jcs.260668</a>
  chicago: Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev,
    Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller.
    “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal
    of Cell Science</i>. The Company of Biologists, 2023. <a href="https://doi.org/10.1242/jcs.260668">https://doi.org/10.1242/jcs.260668</a>.
  ieee: T. Higashi <i>et al.</i>, “ZnUMBA - a live imaging method to detect local
    barrier breaches,” <i>Journal of Cell Science</i>, vol. 136, no. 15. The Company
    of Biologists, 2023.
  ista: Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ,
    Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier
    breaches. Journal of Cell Science. 136(15), jcs260668.
  mla: Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier
    Breaches.” <i>Journal of Cell Science</i>, vol. 136, no. 15, jcs260668, The Company
    of Biologists, 2023, doi:<a href="https://doi.org/10.1242/jcs.260668">10.1242/jcs.260668</a>.
  short: T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J.
    Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).
date_created: 2023-08-20T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2023-12-13T12:11:18Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: EvBe
doi: 10.1242/jcs.260668
ec_funded: 1
external_id:
  isi:
  - '001070149000001'
file:
- access_level: closed
  checksum: a399389b7e3d072f1788b63e612a10b3
  content_type: application/pdf
  creator: dernst
  date_created: 2023-08-21T07:37:54Z
  date_updated: 2023-08-21T07:37:54Z
  embargo: 2024-08-10
  embargo_to: open_access
  file_id: '14092'
  file_name: 2023_JourCellScience_Higashi.pdf
  file_size: 18665315
  relation: main_file
file_date_updated: 2023-08-21T07:37:54Z
has_accepted_license: '1'
intvolume: '       136'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa_version: None
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Journal of Cell Science
publication_identifier:
  eissn:
  - 1477-9137
  issn:
  - 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: ZnUMBA - a live imaging method to detect local barrier breaches
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 136
year: '2023'
...
---
_id: '12830'
abstract:
- lang: eng
  text: Interstitial fluid (IF) accumulation between embryonic cells is thought to
    be important for embryo patterning and morphogenesis. Here, we identify a positive
    mechanical feedback loop between cell migration and IF relocalization and find
    that it promotes embryonic axis formation during zebrafish gastrulation. We show
    that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between
    the yolk cell and deep cell tissue to extend the embryonic axis, compress the
    overlying deep cell layer, thereby causing IF to flow from the deep cell layer
    to the boundary between the yolk cell and the deep cell layer, directly ahead
    of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion
    formation and migration by opening up the space into which the ppl moves and,
    thereby, the ability of the ppl to trigger IF relocalization by pushing against
    the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic
    feedback loop between cell migration and IF relocalization.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful
  discussions and support with the SPIM experiments; the Heisenberg group, and especially
  Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović
  (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and
  zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia
  Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura
  (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work
  was supported by funding from the European Union (European Research Council Advanced
  grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and
  HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des
  deutschen Volkes to F.P.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Diana C
  full_name: Nunes Pinheiro, Diana C
  id: 2E839F16-F248-11E8-B48F-1D18A9856A87
  last_name: Nunes Pinheiro
  orcid: 0000-0003-4333-7503
- first_name: Friedrich
  full_name: Preusser, Friedrich
  last_name: Preusser
- first_name: Irene
  full_name: Steccari, Irene
  id: 2705C766-9FE2-11EA-B224-C6773DDC885E
  last_name: Steccari
- first_name: Christoph M
  full_name: Sommer, Christoph M
  id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
  last_name: Sommer
  orcid: 0000-0003-1216-9105
- first_name: Suyash
  full_name: Naik, Suyash
  id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
  last_name: Naik
  orcid: 0000-0001-8421-5508
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop
    between mesendoderm cell migration and interstitial fluid relocalization promotes
    embryonic axis formation in zebrafish. <i>Developmental Cell</i>. 2023;58(7):582-596.e7.
    doi:<a href="https://doi.org/10.1016/j.devcel.2023.02.016">10.1016/j.devcel.2023.02.016</a>
  apa: Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I.,
    Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between
    mesendoderm cell migration and interstitial fluid relocalization promotes embryonic
    axis formation in zebrafish. <i>Developmental Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.devcel.2023.02.016">https://doi.org/10.1016/j.devcel.2023.02.016</a>
  chicago: Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser,
    Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg.
    “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial
    Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental
    Cell</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.devcel.2023.02.016">https://doi.org/10.1016/j.devcel.2023.02.016</a>.
  ieee: K. Huljev <i>et al.</i>, “A hydraulic feedback loop between mesendoderm cell
    migration and interstitial fluid relocalization promotes embryonic axis formation
    in zebrafish,” <i>Developmental Cell</i>, vol. 58, no. 7. Elsevier, p. 582–596.e7,
    2023.
  ista: Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM,
    Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell
    migration and interstitial fluid relocalization promotes embryonic axis formation
    in zebrafish. Developmental Cell. 58(7), 582–596.e7.
  mla: Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration
    and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.”
    <i>Developmental Cell</i>, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:<a
    href="https://doi.org/10.1016/j.devcel.2023.02.016">10.1016/j.devcel.2023.02.016</a>.
  short: K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M.
    Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.
date_created: 2023-04-16T22:01:07Z
date_published: 2023-04-10T00:00:00Z
date_updated: 2023-08-01T14:10:38Z
day: '10'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1016/j.devcel.2023.02.016
ec_funded: 1
external_id:
  isi:
  - '000982111800001'
file:
- access_level: open_access
  checksum: c80ca2ebc241232aacdb5aa4b4c80957
  content_type: application/pdf
  creator: dernst
  date_created: 2023-04-17T07:41:25Z
  date_updated: 2023-04-17T07:41:25Z
  file_id: '12842'
  file_name: 2023_DevelopmentalCell_Huljev.pdf
  file_size: 7925886
  relation: main_file
  success: 1
file_date_updated: 2023-04-17T07:41:25Z
has_accepted_license: '1'
intvolume: '        58'
isi: 1
issue: '7'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 582-596.e7
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 850-2017
  name: Coordination of mesendoderm cell fate specification and internalization during
    zebrafish gastrulation
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
  grant_number: LT000429
  name: Coordination of mesendoderm fate specification and internalization during
    zebrafish gastrulation
publication: Developmental Cell
publication_identifier:
  eissn:
  - 1878-1551
  issn:
  - 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A hydraulic feedback loop between mesendoderm cell migration and interstitial
  fluid relocalization promotes embryonic axis formation in zebrafish
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 58
year: '2023'
...
---
_id: '9397'
abstract:
- lang: eng
  text: Accumulation of interstitial fluid (IF) between embryonic cells is a common
    phenomenon in vertebrate embryogenesis. Unlike other model systems, where these
    accumulations coalesce into a large central cavity – the blastocoel, in zebrafish,
    IF is more uniformly distributed between the deep cells (DC) before the onset
    of gastrulation. This is likely due to the presence of a large extraembryonic
    structure – the yolk cell (YC) at the position where the blastocoel typically
    forms in other model organisms. IF has long been speculated to play a role in
    tissue morphogenesis during embryogenesis, but direct evidence supporting such
    function is still sparse. Here we show that the relocalization of IF to the interface
    between the YC and DC/epiblast is critical for axial mesendoderm (ME) cell protrusion
    formation and migration along this interface, a key process in embryonic axis
    formation. We further demonstrate that axial ME cell migration and IF relocalization
    engage in a positive feedback loop, where axial ME migration triggers IF accumulation
    ahead of the advancing axial ME tissue by mechanically compressing the overlying
    epiblast cell layer. Upon compression, locally induced flow relocalizes the IF
    through the porous epiblast tissue resulting in an IF accumulation ahead of the
    leading axial ME. This IF accumulation, in turn, promotes cell protrusion formation
    and migration of the leading axial ME cells, thereby facilitating axial ME extension.
    Our findings reveal a central role of dynamic IF relocalization in orchestrating
    germ layer morphogenesis during gastrulation.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
citation:
  ama: Huljev K. Coordinated spatiotemporal reorganization of interstitial fluid is
    required for axial mesendoderm migration in zebrafish gastrulation. 2021. doi:<a
    href="https://doi.org/10.15479/at:ista:9397">10.15479/at:ista:9397</a>
  apa: Huljev, K. (2021). <i>Coordinated spatiotemporal reorganization of interstitial
    fluid is required for axial mesendoderm migration in zebrafish gastrulation</i>.
    Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/at:ista:9397">https://doi.org/10.15479/at:ista:9397</a>
  chicago: Huljev, Karla. “Coordinated Spatiotemporal Reorganization of Interstitial
    Fluid Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation.”
    Institute of Science and Technology Austria, 2021. <a href="https://doi.org/10.15479/at:ista:9397">https://doi.org/10.15479/at:ista:9397</a>.
  ieee: K. Huljev, “Coordinated spatiotemporal reorganization of interstitial fluid
    is required for axial mesendoderm migration in zebrafish gastrulation,” Institute
    of Science and Technology Austria, 2021.
  ista: Huljev K. 2021. Coordinated spatiotemporal reorganization of interstitial
    fluid is required for axial mesendoderm migration in zebrafish gastrulation. Institute
    of Science and Technology Austria.
  mla: Huljev, Karla. <i>Coordinated Spatiotemporal Reorganization of Interstitial
    Fluid Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation</i>.
    Institute of Science and Technology Austria, 2021, doi:<a href="https://doi.org/10.15479/at:ista:9397">10.15479/at:ista:9397</a>.
  short: K. Huljev, Coordinated Spatiotemporal Reorganization of Interstitial Fluid
    Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation, Institute
    of Science and Technology Austria, 2021.
date_created: 2021-05-17T12:31:30Z
date_published: 2021-05-18T00:00:00Z
date_updated: 2023-09-07T13:32:32Z
day: '18'
ddc:
- '571'
degree_awarded: PhD
department:
- _id: CaHe
- _id: GradSch
doi: 10.15479/at:ista:9397
file:
- access_level: closed
  checksum: 7f98532f5324a0b2f3fa8de2967baa19
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: khuljev
  date_created: 2021-05-17T12:29:12Z
  date_updated: 2022-05-21T22:30:04Z
  embargo_to: open_access
  file_id: '9398'
  file_name: KHuljev_Thesis_corrections.docx
  file_size: 47799741
  relation: source_file
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  checksum: bf512f8a1e572a543778fc4b227c01ba
  content_type: application/pdf
  creator: khuljev
  date_created: 2021-05-18T14:50:28Z
  date_updated: 2022-05-21T22:30:04Z
  embargo: 2022-05-20
  file_id: '9401'
  file_name: new_KHuljev_Thesis_corrections.pdf
  file_size: 16542131
  relation: main_file
file_date_updated: 2022-05-21T22:30:04Z
has_accepted_license: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: '101'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: Coordinated spatiotemporal reorganization of interstitial fluid is required
  for axial mesendoderm migration in zebrafish gastrulation
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2021'
...
---
_id: '9750'
abstract:
- lang: eng
  text: Tension of the actomyosin cell cortex plays a key role in determining cell-cell
    contact growth and size. The level of cortical tension outside of the cell-cell
    contact, when pulling at the contact edge, scales with the total size to which
    a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer
    progenitor cells that this monotonic relationship only applies to a narrow range
    of cortical tension increase, and that above a critical threshold, contact size
    inversely scales with cortical tension. This switch from cortical tension increasing
    to decreasing progenitor cell-cell contact size is caused by cortical tension
    promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing
    clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin
    stabilization at the contact exceeds a critical threshold level, the rate by which
    the contact expands in response to pulling forces from the cortex sharply drops,
    leading to smaller contacts at physiologically relevant timescales of contact
    formation. Thus, the activity of cortical tension in expanding cell-cell contact
    size is limited by tension stabilizing E-cadherin-actin complexes at the contact.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: SSU
acknowledgement: We would like to thank Edouard Hannezo for discussions, Shayan Shami
  Pour and Daniel Capek for help with data analysis, Vanessa Barone and other members
  of the Heisenberg laboratory for thoughtful discussions and comments on the manuscript.
  We also thank Jack Merrin for preparing the microwells, and the Scientific Service
  Units at IST Austria, specifically Bioimaging and Electron Microscopy, and the Zebrafish
  Facility for continuous support. We acknowledge Hitoshi Morita for the kind gift
  of VinculinB-GFP plasmid. This research was supported by an ERC Advanced Grant (MECSPEC)
  to C.-P.H, EMBO Long Term grant (ALTF 187-2013) to M.S and IST Fellow Marie-Curie
  COFUND No. P_IST_EU01 to J.S.
article_processing_charge: No
author:
- first_name: Jana
  full_name: Slovakova, Jana
  id: 30F3F2F0-F248-11E8-B48F-1D18A9856A87
  last_name: Slovakova
- first_name: Mateusz K
  full_name: Sikora, Mateusz K
  id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
  last_name: Sikora
- first_name: Silvia
  full_name: Caballero Mancebo, Silvia
  id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
  last_name: Caballero Mancebo
  orcid: 0000-0002-5223-3346
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization
    of E-cadherin limits cell-cell contact expansion. <i>bioRxiv</i>. 2020. doi:<a
    href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a>
  apa: Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W.,
    Huljev, K., &#38; Heisenberg, C.-P. J. (2020). Tension-dependent stabilization
    of E-cadherin limits cell-cell contact expansion. <i>bioRxiv</i>. Cold Spring
    Harbor Laboratory. <a href="https://doi.org/10.1101/2020.11.20.391284">https://doi.org/10.1101/2020.11.20.391284</a>
  chicago: Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens,
    Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent
    Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” <i>BioRxiv</i>.
    Cold Spring Harbor Laboratory, 2020. <a href="https://doi.org/10.1101/2020.11.20.391284">https://doi.org/10.1101/2020.11.20.391284</a>.
  ieee: J. Slovakova <i>et al.</i>, “Tension-dependent stabilization of E-cadherin
    limits cell-cell contact expansion,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory,
    2020.
  ista: Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K,
    Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell
    contact expansion. bioRxiv, <a href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a>.
  mla: Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits
    Cell-Cell Contact Expansion.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, 2020,
    doi:<a href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a>.
  short: J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K.
    Huljev, C.-P.J. Heisenberg, BioRxiv (2020).
date_created: 2021-07-29T11:29:50Z
date_published: 2020-11-20T00:00:00Z
date_updated: 2024-03-25T23:30:10Z
day: '20'
department:
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- _id: EM-Fac
- _id: Bio
doi: 10.1101/2020.11.20.391284
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language:
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month: '11'
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oa_version: Preprint
page: '41'
project:
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publication: bioRxiv
publication_status: published
publisher: Cold Spring Harbor Laboratory
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status: public
title: Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2020'
...