---
_id: '15048'
abstract:
- lang: eng
  text: Embryogenesis results from the coordinated activities of different signaling
    pathways controlling cell fate specification and morphogenesis. In vertebrate
    gastrulation, both Nodal and BMP signaling play key roles in germ layer specification
    and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis
    is still insufficiently understood. Here, we took a reductionist approach using
    zebrafish embryonic explants to study the coordination of Nodal and BMP signaling
    for embryo patterning and morphogenesis. We show that Nodal signaling triggers
    explant elongation by inducing mesendodermal progenitors but also suppressing
    BMP signaling activity at the site of mesendoderm induction. Consistent with this,
    ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm
    intercalations, key processes during explant elongation. Translating these ex
    vivo observations to the intact embryo showed that, similar to explants, Nodal
    signaling suppresses the effect of BMP signaling on cell intercalations in the
    dorsal domain, thus allowing robust embryonic axis elongation. These findings
    suggest a dual function of Nodal signaling in embryonic axis elongation by both
    inducing mesendoderm and suppressing BMP effects in the dorsal portion of the
    mesendoderm.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Patrick Müller for sharing the chordintt250 mutant zebrafish
  line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b
  plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated
  the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro
  and Katherine Rogers and members of the Heisenberg lab for discussions, technical
  advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo
  for discussions. We thank the Imaging and Optics Facility as well as the Life Science
  facility at IST Austria for support with microscopy and fish maintenance.\r\nThis
  work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573
  to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy
  of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience
  and Technology Austria. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation
    by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18.
    doi:<a href="https://doi.org/10.1242/dev.202316">10.1242/dev.202316</a>
  apa: Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J.
    (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling.
    <i>Development</i>. The Company of Biologists. <a href="https://doi.org/10.1242/dev.202316">https://doi.org/10.1242/dev.202316</a>
  chicago: Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp
    J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.”
    <i>Development</i>. The Company of Biologists, 2024. <a href="https://doi.org/10.1242/dev.202316">https://doi.org/10.1242/dev.202316</a>.
  ieee: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust
    axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>,
    vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.
  ista: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis
    elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4),
    1–18.
  mla: Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction
    of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, The Company of Biologists,
    2024, pp. 1–18, doi:<a href="https://doi.org/10.1242/dev.202316">10.1242/dev.202316</a>.
  short: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development
    151 (2024) 1–18.
date_created: 2024-03-03T23:00:50Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2024-03-04T07:28:25Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1242/dev.202316
ec_funded: 1
file:
- access_level: open_access
  checksum: 6961ea10012bf0d266681f9628bb8f13
  content_type: application/pdf
  creator: dernst
  date_created: 2024-03-04T07:24:43Z
  date_updated: 2024-03-04T07:24:43Z
  file_id: '15050'
  file_name: 2024_Development_Schauer.pdf
  file_size: 14839986
  relation: main_file
  success: 1
file_date_updated: 2024-03-04T07:24:43Z
has_accepted_license: '1'
intvolume: '       151'
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: 1-18
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Development
publication_identifier:
  eissn:
  - 1477-9129
  issn:
  - 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
  record:
  - id: '14926'
    relation: research_data
    status: public
scopus_import: '1'
status: public
title: Robust axis elongation by Nodal-dependent restriction of BMP signaling
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 151
year: '2024'
...
---
_id: '7001'
acknowledged_ssus:
- _id: PreCl
- _id: Bio
article_processing_charge: No
article_type: original
author:
- first_name: Cornelia
  full_name: Schwayer, Cornelia
  id: 3436488C-F248-11E8-B48F-1D18A9856A87
  last_name: Schwayer
  orcid: 0000-0001-5130-2226
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: M
  full_name: Balda, M
  last_name: Balda
- first_name: M
  full_name: Tada, M
  last_name: Tada
- first_name: K
  full_name: Matter, K
  last_name: Matter
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schwayer C, Shamipour S, Pranjic-Ferscha K, et al. Mechanosensation of tight
    junctions depends on ZO-1 phase separation and flow. <i>Cell</i>. 2019;179(4):937-952.e18.
    doi:<a href="https://doi.org/10.1016/j.cell.2019.10.006">10.1016/j.cell.2019.10.006</a>
  apa: Schwayer, C., Shamipour, S., Pranjic-Ferscha, K., Schauer, A., Balda, M., Tada,
    M., … Heisenberg, C.-P. J. (2019). Mechanosensation of tight junctions depends
    on ZO-1 phase separation and flow. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2019.10.006">https://doi.org/10.1016/j.cell.2019.10.006</a>
  chicago: Schwayer, Cornelia, Shayan Shamipour, Kornelija Pranjic-Ferscha, Alexandra
    Schauer, M Balda, M Tada, K Matter, and Carl-Philipp J Heisenberg. “Mechanosensation
    of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” <i>Cell</i>. Cell
    Press, 2019. <a href="https://doi.org/10.1016/j.cell.2019.10.006">https://doi.org/10.1016/j.cell.2019.10.006</a>.
  ieee: C. Schwayer <i>et al.</i>, “Mechanosensation of tight junctions depends on
    ZO-1 phase separation and flow,” <i>Cell</i>, vol. 179, no. 4. Cell Press, p.
    937–952.e18, 2019.
  ista: Schwayer C, Shamipour S, Pranjic-Ferscha K, Schauer A, Balda M, Tada M, Matter
    K, Heisenberg C-PJ. 2019. Mechanosensation of tight junctions depends on ZO-1
    phase separation and flow. Cell. 179(4), 937–952.e18.
  mla: Schwayer, Cornelia, et al. “Mechanosensation of Tight Junctions Depends on
    ZO-1 Phase Separation and Flow.” <i>Cell</i>, vol. 179, no. 4, Cell Press, 2019,
    p. 937–952.e18, doi:<a href="https://doi.org/10.1016/j.cell.2019.10.006">10.1016/j.cell.2019.10.006</a>.
  short: C. Schwayer, S. Shamipour, K. Pranjic-Ferscha, A. Schauer, M. Balda, M. Tada,
    K. Matter, C.-P.J. Heisenberg, Cell 179 (2019) 937–952.e18.
date_created: 2019-11-12T12:51:06Z
date_published: 2019-10-31T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '31'
ddc:
- '570'
department:
- _id: CaHe
- _id: BjHo
doi: 10.1016/j.cell.2019.10.006
ec_funded: 1
external_id:
  isi:
  - '000493898000012'
  pmid:
  - '31675500'
file:
- access_level: open_access
  checksum: 33dac4bb77ee630e2666e936b4d57980
  content_type: application/pdf
  creator: dernst
  date_created: 2020-10-21T07:09:45Z
  date_updated: 2020-10-21T07:09:45Z
  file_id: '8684'
  file_name: 2019_Cell_Schwayer_accepted.pdf
  file_size: 8805878
  relation: main_file
  success: 1
file_date_updated: 2020-10-21T07:09:45Z
has_accepted_license: '1'
intvolume: '       179'
isi: 1
issue: '4'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 937-952.e18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Cell
publication_identifier:
  eissn:
  - 1097-4172
  issn:
  - 0092-8674
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
  link:
  - description: News auf IST Website
    relation: press_release
    url: https://ist.ac.at/en/news/biochemistry-meets-mechanics-the-sensitive-nature-of-cell-cell-contact-formation-in-embryo-development/
  record:
  - id: '7186'
    relation: dissertation_contains
    status: public
  - id: '8350'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Mechanosensation of tight junctions depends on ZO-1 phase separation and flow
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 179
year: '2019'
...
---
_id: '1912'
abstract:
- lang: eng
  text: Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the
    embryo along its left-right (LR) axis. Regional differences in cell shape within
    the lumen-lining KV epithelium are essential for its LR patterning function. However,
    the processes by which KV cells acquire their characteristic shapes are largely
    unknown. Here, we show that the notochord induces regional differences in cell
    shape within KV by triggering extracellular matrix (ECM) accumulation adjacent
    to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts
    apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen
    growth. Our study provides mechanistic insight into the processes by which KV
    translates global embryonic patterning into regional cell shape differences required
    for its LR symmetry-breaking function.
acknowledgement: We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus,
  and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing
  reagents. This work was supported by the Institute of Science and Technology Austria
  and an Alexander von Humboldt Foundation fellowship to J.C.
article_processing_charge: No
author:
- first_name: Julien
  full_name: Compagnon, Julien
  id: 2E3E0988-F248-11E8-B48F-1D18A9856A87
  last_name: Compagnon
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Srivarsha
  full_name: Rajshekar, Srivarsha
  last_name: Rajshekar
- first_name: Rita
  full_name: Kottmeier, Rita
  last_name: Kottmeier
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry
    by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental
    Cell</i>. 2014;31(6):774-783. doi:<a href="https://doi.org/10.1016/j.devcel.2014.11.003">10.1016/j.devcel.2014.11.003</a>
  apa: Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K.,
    Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). The notochord breaks bilateral
    symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental
    Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.devcel.2014.11.003">https://doi.org/10.1016/j.devcel.2014.11.003</a>
  chicago: Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier,
    Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The
    Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish
    Laterality Organ.” <i>Developmental Cell</i>. Cell Press, 2014. <a href="https://doi.org/10.1016/j.devcel.2014.11.003">https://doi.org/10.1016/j.devcel.2014.11.003</a>.
  ieee: J. Compagnon <i>et al.</i>, “The notochord breaks bilateral symmetry by controlling
    cell shapes in the Zebrafish laterality organ,” <i>Developmental Cell</i>, vol.
    31, no. 6. Cell Press, pp. 774–783, 2014.
  ista: Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt
    M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling
    cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783.
  mla: Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling
    Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>, vol.
    31, no. 6, Cell Press, 2014, pp. 774–83, doi:<a href="https://doi.org/10.1016/j.devcel.2014.11.003">10.1016/j.devcel.2014.11.003</a>.
  short: J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha,
    M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.
date_created: 2018-12-11T11:54:41Z
date_published: 2014-12-22T00:00:00Z
date_updated: 2023-09-07T12:05:08Z
day: '22'
department:
- _id: CaHe
doi: 10.1016/j.devcel.2014.11.003
external_id:
  pmid:
  - '25535919'
intvolume: '        31'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pubmed/25535919
month: '12'
oa: 1
oa_version: Published Version
page: 774 - 783
pmid: 1
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '5182'
quality_controlled: '1'
related_material:
  record:
  - id: '961'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish
  laterality organ
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 31
year: '2014'
...