---
_id: '308'
abstract:
- lang: eng
  text: Migrating cells penetrate tissue barriers during development, inflammatory
    responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally
    confined environments requires changes in the mechanical properties of the surrounding
    cells using embryonic Drosophila melanogaster hemocytes, also called macrophages,
    as a model. We find that macrophage invasion into the germband through transient
    separation of the apposing ectoderm and mesoderm requires cell deformations and
    reductions in apical tension in the ectoderm. Interestingly, the genetic pathway
    governing these mechanical shifts acts downstream of the only known tumor necrosis
    factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald.
    Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal
    cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated
    tight junction protein). We therefore elucidate a distinct molecular pathway that
    controls tissue tension and demonstrate the importance of such regulation for
    invasive migration in vivo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: original
author:
- first_name: Aparna
  full_name: Ratheesh, Aparna
  id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
  last_name: Ratheesh
  orcid: 0000-0001-7190-0776
- first_name: Julia
  full_name: Biebl, Julia
  id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
  last_name: Biebl
- first_name: Michael
  full_name: Smutny, Michael
  last_name: Smutny
- first_name: Jana
  full_name: Veselá, Jana
  id: 433253EE-F248-11E8-B48F-1D18A9856A87
  last_name: Veselá
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Attila
  full_name: György, Attila
  id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
  last_name: György
  orcid: 0000-0002-1819-198X
- first_name: Alessandra M
  full_name: Casano, Alessandra M
  id: 3DBA3F4E-F248-11E8-B48F-1D18A9856A87
  last_name: Casano
  orcid: 0000-0002-6009-6804
- first_name: Daria E
  full_name: Siekhaus, Daria E
  id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
  last_name: Siekhaus
  orcid: 0000-0001-8323-8353
citation:
  ama: Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension
    in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>.
    2018;45(3):331-346. doi:<a href="https://doi.org/10.1016/j.devcel.2018.04.002">10.1016/j.devcel.2018.04.002</a>
  apa: Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G.,
    … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo
    to facilitate macrophage invasive migration. <i>Developmental Cell</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.devcel.2018.04.002">https://doi.org/10.1016/j.devcel.2018.04.002</a>
  chicago: Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina
    Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano,
    and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to
    Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>. Elsevier,
    2018. <a href="https://doi.org/10.1016/j.devcel.2018.04.002">https://doi.org/10.1016/j.devcel.2018.04.002</a>.
  ieee: A. Ratheesh <i>et al.</i>, “Drosophila TNF modulates tissue tension in the
    embryo to facilitate macrophage invasive migration,” <i>Developmental Cell</i>,
    vol. 45, no. 3. Elsevier, pp. 331–346, 2018.
  ista: Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W,
    György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension
    in the embryo to facilitate macrophage invasive migration. Developmental Cell.
    45(3), 331–346.
  mla: Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo
    to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>, vol.
    45, no. 3, Elsevier, 2018, pp. 331–46, doi:<a href="https://doi.org/10.1016/j.devcel.2018.04.002">10.1016/j.devcel.2018.04.002</a>.
  short: A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W.
    Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018)
    331–346.
date_created: 2018-12-11T11:45:44Z
date_published: 2018-05-07T00:00:00Z
date_updated: 2023-09-11T13:22:13Z
day: '07'
department:
- _id: DaSi
- _id: CaHe
- _id: Bio
- _id: EM-Fac
- _id: MiSi
doi: 10.1016/j.devcel.2018.04.002
ec_funded: 1
external_id:
  isi:
  - '000432461400009'
  pmid:
  - '29738712'
intvolume: '        45'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.devcel.2018.04.002
month: '05'
oa: 1
oa_version: Published Version
page: 331 - 346
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29638
  name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '334077'
  name: Investigating the role of transporters in invasive migration through junctions
publication: Developmental Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/
scopus_import: '1'
status: public
title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage
  invasive migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2018'
...