---
_id: '308'
abstract:
- lang: eng
  text: Migrating cells penetrate tissue barriers during development, inflammatory
    responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally
    confined environments requires changes in the mechanical properties of the surrounding
    cells using embryonic Drosophila melanogaster hemocytes, also called macrophages,
    as a model. We find that macrophage invasion into the germband through transient
    separation of the apposing ectoderm and mesoderm requires cell deformations and
    reductions in apical tension in the ectoderm. Interestingly, the genetic pathway
    governing these mechanical shifts acts downstream of the only known tumor necrosis
    factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald.
    Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal
    cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated
    tight junction protein). We therefore elucidate a distinct molecular pathway that
    controls tissue tension and demonstrate the importance of such regulation for
    invasive migration in vivo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: original
author:
- first_name: Aparna
  full_name: Ratheesh, Aparna
  id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
  last_name: Ratheesh
  orcid: 0000-0001-7190-0776
- first_name: Julia
  full_name: Biebl, Julia
  id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
  last_name: Biebl
- first_name: Michael
  full_name: Smutny, Michael
  last_name: Smutny
- first_name: Jana
  full_name: Veselá, Jana
  id: 433253EE-F248-11E8-B48F-1D18A9856A87
  last_name: Veselá
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Attila
  full_name: György, Attila
  id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
  last_name: György
  orcid: 0000-0002-1819-198X
- first_name: Alessandra M
  full_name: Casano, Alessandra M
  id: 3DBA3F4E-F248-11E8-B48F-1D18A9856A87
  last_name: Casano
  orcid: 0000-0002-6009-6804
- first_name: Daria E
  full_name: Siekhaus, Daria E
  id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
  last_name: Siekhaus
  orcid: 0000-0001-8323-8353
citation:
  ama: Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension
    in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>.
    2018;45(3):331-346. doi:<a href="https://doi.org/10.1016/j.devcel.2018.04.002">10.1016/j.devcel.2018.04.002</a>
  apa: Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G.,
    … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo
    to facilitate macrophage invasive migration. <i>Developmental Cell</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.devcel.2018.04.002">https://doi.org/10.1016/j.devcel.2018.04.002</a>
  chicago: Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina
    Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano,
    and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to
    Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>. Elsevier,
    2018. <a href="https://doi.org/10.1016/j.devcel.2018.04.002">https://doi.org/10.1016/j.devcel.2018.04.002</a>.
  ieee: A. Ratheesh <i>et al.</i>, “Drosophila TNF modulates tissue tension in the
    embryo to facilitate macrophage invasive migration,” <i>Developmental Cell</i>,
    vol. 45, no. 3. Elsevier, pp. 331–346, 2018.
  ista: Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W,
    György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension
    in the embryo to facilitate macrophage invasive migration. Developmental Cell.
    45(3), 331–346.
  mla: Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo
    to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>, vol.
    45, no. 3, Elsevier, 2018, pp. 331–46, doi:<a href="https://doi.org/10.1016/j.devcel.2018.04.002">10.1016/j.devcel.2018.04.002</a>.
  short: A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W.
    Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018)
    331–346.
date_created: 2018-12-11T11:45:44Z
date_published: 2018-05-07T00:00:00Z
date_updated: 2023-09-11T13:22:13Z
day: '07'
department:
- _id: DaSi
- _id: CaHe
- _id: Bio
- _id: EM-Fac
- _id: MiSi
doi: 10.1016/j.devcel.2018.04.002
ec_funded: 1
external_id:
  isi:
  - '000432461400009'
  pmid:
  - '29738712'
intvolume: '        45'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.devcel.2018.04.002
month: '05'
oa: 1
oa_version: Published Version
page: 331 - 346
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29638
  name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '334077'
  name: Investigating the role of transporters in invasive migration through junctions
publication: Developmental Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/
scopus_import: '1'
status: public
title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage
  invasive migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2018'
...
---
_id: '661'
abstract:
- lang: eng
  text: During embryonic development, mechanical forces are essential for cellular
    rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish
    embryo, friction forces are generated at the interface between anterior axial
    mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole
    and neurectoderm progenitors moving in the opposite direction towards the vegetal
    pole of the embryo. These friction forces lead to global rearrangement of cells
    within the neurectoderm and determine the position of the neural anlage. Using
    a combination of experiments and simulations, we show that this process depends
    on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated
    adhesion between those tissues. Our data thus establish the emergence of friction
    forces at the interface between moving tissues as a critical force-generating
    process shaping the embryo.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Zsuzsa
  full_name: Ákos, Zsuzsa
  last_name: Ákos
- first_name: Silvia
  full_name: Grigolon, Silvia
  last_name: Grigolon
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Verena
  full_name: Ruprecht, Verena
  last_name: Ruprecht
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Masazumi
  full_name: Tada, Masazumi
  last_name: Tada
- first_name: Björn
  full_name: Hof, Björn
  id: 3A374330-F248-11E8-B48F-1D18A9856A87
  last_name: Hof
  orcid: 0000-0003-2057-2754
- first_name: Tamás
  full_name: Vicsek, Tamás
  last_name: Vicsek
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage.
    <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>
  apa: Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D.,
    … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature
    Cell Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>
  chicago: Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena
    Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural
    Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>.
  ieee: M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature
    Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.
  ista: Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M,
    Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction
    forces position the neural anlage. Nature Cell Biology. 19, 306–317.
  mla: Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature
    Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>.
  short: M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M.
    Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg,
    Nature Cell Biology 19 (2017) 306–317.
date_created: 2018-12-11T11:47:46Z
date_published: 2017-03-27T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '27'
department:
- _id: CaHe
- _id: BjHo
- _id: Bio
doi: 10.1038/ncb3492
ec_funded: 1
external_id:
  pmid:
  - '28346437'
intvolume: '        19'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://europepmc.org/articles/pmc5635970
month: '03'
oa: 1
oa_version: Submitted Version
page: 306 - 317
pmid: 1
project:
- _id: 25152F3A-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '306589'
  name: Decoding the complexity of turbulence at its origin
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_identifier:
  issn:
  - '14657392'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7074'
quality_controlled: '1'
related_material:
  record:
  - id: '50'
    relation: dissertation_contains
    status: public
  - id: '8350'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Friction forces position the neural anlage
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2017'
...
---
_id: '2022'
abstract:
- lang: eng
  text: Radial glial progenitors (RGPs) are responsible for producing nearly all neocortical
    neurons. To gain insight into the patterns of RGP division and neuron production,
    we quantitatively analyzed excitatory neuron genesis in the mouse neocortex using
    Mosaic Analysis with Double Markers, which provides single-cell resolution of
    progenitor division patterns and potential in vivo. We found that RGPs progress
    through a coherent program in which their proliferative potential diminishes in
    a predictable manner. Upon entry into the neurogenic phase, individual RGPs produce
    ∼8–9 neurons distributed in both deep and superficial layers, indicating a unitary
    output in neuronal production. Removal of OTX1, a transcription factor transiently
    expressed in RGPs, results in both deep- and superficial-layer neuron loss and
    a reduction in neuronal unit size. Moreover, ∼1/6 of neurogenic RGPs proceed to
    produce glia. These results suggest that progenitor behavior and histogenesis
    in the mammalian neocortex conform to a remarkably orderly and deterministic program.
author:
- first_name: Peng
  full_name: Gao, Peng
  last_name: Gao
- first_name: Maria P
  full_name: Postiglione, Maria P
  id: 2C67902A-F248-11E8-B48F-1D18A9856A87
  last_name: Postiglione
- first_name: Teresa
  full_name: Krieger, Teresa
  last_name: Krieger
- first_name: Luisirene
  full_name: Hernandez, Luisirene
  last_name: Hernandez
- first_name: Chao
  full_name: Wang, Chao
  last_name: Wang
- first_name: Zhi
  full_name: Han, Zhi
  last_name: Han
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Ryan
  full_name: Insolera, Ryan
  last_name: Insolera
- first_name: Kritika
  full_name: Chugh, Kritika
  last_name: Chugh
- first_name: Oren
  full_name: Kodish, Oren
  last_name: Kodish
- first_name: Kun
  full_name: Huang, Kun
  last_name: Huang
- first_name: Benjamin
  full_name: Simons, Benjamin
  last_name: Simons
- first_name: Liqun
  full_name: Luo, Liqun
  last_name: Luo
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Song
  full_name: Shi, Song
  last_name: Shi
citation:
  ama: Gao P, Postiglione MP, Krieger T, et al. Deterministic progenitor behavior
    and unitary production of neurons in the neocortex. <i>Cell</i>. 2014;159(4):775-788.
    doi:<a href="https://doi.org/10.1016/j.cell.2014.10.027">10.1016/j.cell.2014.10.027</a>
  apa: Gao, P., Postiglione, M. P., Krieger, T., Hernandez, L., Wang, C., Han, Z.,
    … Shi, S. (2014). Deterministic progenitor behavior and unitary production of
    neurons in the neocortex. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2014.10.027">https://doi.org/10.1016/j.cell.2014.10.027</a>
  chicago: Gao, Peng, Maria P Postiglione, Teresa Krieger, Luisirene Hernandez, Chao
    Wang, Zhi Han, Carmen Streicher, et al. “Deterministic Progenitor Behavior and
    Unitary Production of Neurons in the Neocortex.” <i>Cell</i>. Cell Press, 2014.
    <a href="https://doi.org/10.1016/j.cell.2014.10.027">https://doi.org/10.1016/j.cell.2014.10.027</a>.
  ieee: P. Gao <i>et al.</i>, “Deterministic progenitor behavior and unitary production
    of neurons in the neocortex,” <i>Cell</i>, vol. 159, no. 4. Cell Press, pp. 775–788,
    2014.
  ista: Gao P, Postiglione MP, Krieger T, Hernandez L, Wang C, Han Z, Streicher C,
    Papusheva E, Insolera R, Chugh K, Kodish O, Huang K, Simons B, Luo L, Hippenmeyer
    S, Shi S. 2014. Deterministic progenitor behavior and unitary production of neurons
    in the neocortex. Cell. 159(4), 775–788.
  mla: Gao, Peng, et al. “Deterministic Progenitor Behavior and Unitary Production
    of Neurons in the Neocortex.” <i>Cell</i>, vol. 159, no. 4, Cell Press, 2014,
    pp. 775–88, doi:<a href="https://doi.org/10.1016/j.cell.2014.10.027">10.1016/j.cell.2014.10.027</a>.
  short: P. Gao, M.P. Postiglione, T. Krieger, L. Hernandez, C. Wang, Z. Han, C. Streicher,
    E. Papusheva, R. Insolera, K. Chugh, O. Kodish, K. Huang, B. Simons, L. Luo, S.
    Hippenmeyer, S. Shi, Cell 159 (2014) 775–788.
date_created: 2018-12-11T11:55:16Z
date_published: 2014-11-06T00:00:00Z
date_updated: 2021-01-12T06:54:47Z
day: '06'
ddc:
- '570'
department:
- _id: SiHi
- _id: Bio
doi: 10.1016/j.cell.2014.10.027
ec_funded: 1
file:
- access_level: open_access
  checksum: 6c5de8329bb2ffa71cba9fda750f14ce
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:08:47Z
  date_updated: 2020-07-14T12:45:25Z
  file_id: '4709'
  file_name: IST-2016-423-v1+1_1-s2.0-S0092867414013154-main.pdf
  file_size: 4435787
  relation: main_file
file_date_updated: 2020-07-14T12:45:25Z
has_accepted_license: '1'
intvolume: '       159'
issue: '4'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 775 - 788
project:
- _id: 25D61E48-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '618444'
  name: Molecular Mechanisms of Cerebral Cortex Development
- _id: 25D7962E-B435-11E9-9278-68D0E5697425
  grant_number: RGP0053/2014
  name: Quantitative Structure-Function Analysis of Cerebral Cortex Assembly at Clonal
    Level
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5050'
pubrep_id: '423'
quality_controlled: '1'
scopus_import: 1
status: public
title: Deterministic progenitor behavior and unitary production of neurons in the
  neocortex
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 159
year: '2014'
...
---
_id: '4157'
abstract:
- lang: eng
  text: Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis,
    allowing cells and tissues to change shape without loosing integrity. Studies
    predominantly in cell culture showed that mechanosensation through adhesion structures
    is achieved by force-mediated modulation of their molecular composition. The specific
    molecular composition of adhesion sites in turn determines their signalling activity
    and dynamic reorganization. Here, we will review how adhesion sites respond to
    mecanical stimuli, and how spatially and temporally regulated signalling from
    different adhesion sites controls cell migration and tissue morphogenesis.
acknowledged_ssus:
- _id: Bio
author:
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent
    regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. 2010;29(16):2753-2768.
    doi:<a href="https://doi.org/10.1038/emboj.2010.182">10.1038/emboj.2010.182</a>'
  apa: 'Papusheva, E., &#38; Heisenberg, C.-P. J. (2010). Spatial organization of
    adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO
    Journal</i>. Wiley-Blackwell. <a href="https://doi.org/10.1038/emboj.2010.182">https://doi.org/10.1038/emboj.2010.182</a>'
  chicago: 'Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization
    of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
    <i>EMBO Journal</i>. Wiley-Blackwell, 2010. <a href="https://doi.org/10.1038/emboj.2010.182">https://doi.org/10.1038/emboj.2010.182</a>.'
  ieee: 'E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion:
    force-dependent regulation and function in tissue morphogenesis,” <i>EMBO Journal</i>,
    vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010.'
  ista: 'Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent
    regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.'
  mla: 'Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization
    of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
    <i>EMBO Journal</i>, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:<a
    href="https://doi.org/10.1038/emboj.2010.182">10.1038/emboj.2010.182</a>.'
  short: E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.
date_created: 2018-12-11T12:07:17Z
date_published: 2010-08-18T00:00:00Z
date_updated: 2021-01-12T07:54:55Z
day: '18'
department:
- _id: Bio
- _id: CaHe
doi: 10.1038/emboj.2010.182
external_id:
  pmid:
  - '20717145'
intvolume: '        29'
issue: '16'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/
month: '08'
oa: 1
oa_version: Submitted Version
page: 2753 - 2768
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1962'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Spatial organization of adhesion: force-dependent regulation and function
  in tissue morphogenesis'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2010'
...
---
_id: '4187'
abstract:
- lang: eng
  text: Cell migration is central to embryonic development, homeostasis and disease(1),
    processes in which cells move as part of a group or individually. Whereas the
    mechanisms controlling single-cell migration in vitro are relatively well understood(2-4),
    less is known about the mechanisms promoting the motility of individual cells
    in vivo. In particular, it is not clear how cells that form blebs in their migration
    use those protrusions to bring about movement in the context of the three-dimensional
    cellular environment(5,6). Here we show that the motility of chemokine-guided
    germ cells within the zebrafish embryo requires the function of the small Rho
    GTPases Rac1 and RhoA, as well as E-cadherin-mediated cell-cell adhesion. Using
    fluorescence resonance energy transfer we demonstrate that Rac1 and RhoA are activated
    in the cell front. At this location, Rac1 is responsible for the formation of
    actin-rich structures, and RhoA promotes retrograde actin flow. We propose that
    these actin-rich structures undergoing retrograde flow are essential for the generation
    of E-cadherin-mediated traction forces between the germ cells and the surrounding
    tissue and are therefore crucial for cell motility in vivo.
author:
- first_name: Elena
  full_name: Kardash, Elena
  last_name: Kardash
- first_name: Michal
  full_name: Reichman-Fried, Michal
  last_name: Reichman Fried
- first_name: Jean
  full_name: Maître, Jean-Léon
  last_name: Maître
- first_name: Bijan
  full_name: Boldajipour, Bijan
  last_name: Boldajipour
- first_name: Ekaterina
  full_name: Ekaterina Papusheva
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Esther
  full_name: Messerschmidt, Esther-Maria
  last_name: Messerschmidt
- first_name: Carl
  full_name: Heisenberg, Carl-Philipp
  last_name: Heisenberg
- first_name: Erez
  full_name: Raz, Erez
  last_name: Raz
citation:
  ama: Kardash E, Reichman Fried M, Maître J, et al. A role for Rho GTPases and cell-cell
    adhesion in single-cell motility in vivo. <i>Nature Cell Biology</i>. 2010;12(1):47-53.
    doi:<a href="https://doi.org/10.1038/ncb2003">10.1038/ncb2003</a>
  apa: Kardash, E., Reichman Fried, M., Maître, J., Boldajipour, B., Papusheva, E.,
    Messerschmidt, E., … Raz, E. (2010). A role for Rho GTPases and cell-cell adhesion
    in single-cell motility in vivo. <i>Nature Cell Biology</i>. Nature Publishing
    Group. <a href="https://doi.org/10.1038/ncb2003">https://doi.org/10.1038/ncb2003</a>
  chicago: Kardash, Elena, Michal Reichman Fried, Jean Maître, Bijan Boldajipour,
    Ekaterina Papusheva, Esther Messerschmidt, Carl Heisenberg, and Erez Raz. “A Role
    for Rho GTPases and Cell-Cell Adhesion in Single-Cell Motility in Vivo.” <i>Nature
    Cell Biology</i>. Nature Publishing Group, 2010. <a href="https://doi.org/10.1038/ncb2003">https://doi.org/10.1038/ncb2003</a>.
  ieee: E. Kardash <i>et al.</i>, “A role for Rho GTPases and cell-cell adhesion in
    single-cell motility in vivo,” <i>Nature Cell Biology</i>, vol. 12, no. 1. Nature
    Publishing Group, pp. 47–53, 2010.
  ista: Kardash E, Reichman Fried M, Maître J, Boldajipour B, Papusheva E, Messerschmidt
    E, Heisenberg C, Raz E. 2010. A role for Rho GTPases and cell-cell adhesion in
    single-cell motility in vivo. Nature Cell Biology. 12(1), 47–53.
  mla: Kardash, Elena, et al. “A Role for Rho GTPases and Cell-Cell Adhesion in Single-Cell
    Motility in Vivo.” <i>Nature Cell Biology</i>, vol. 12, no. 1, Nature Publishing
    Group, 2010, pp. 47–53, doi:<a href="https://doi.org/10.1038/ncb2003">10.1038/ncb2003</a>.
  short: E. Kardash, M. Reichman Fried, J. Maître, B. Boldajipour, E. Papusheva, E.
    Messerschmidt, C. Heisenberg, E. Raz, Nature Cell Biology 12 (2010) 47–53.
date_created: 2018-12-11T12:07:28Z
date_published: 2010-01-01T00:00:00Z
date_updated: 2021-01-12T07:55:09Z
day: '01'
doi: 10.1038/ncb2003
extern: 1
intvolume: '        12'
issue: '1'
month: '01'
page: 47 - 53
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '1932'
quality_controlled: 0
status: public
title: A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo
type: journal_article
volume: 12
year: '2010'
...