[{"pmid":1,"ec_funded":1,"date_published":"2018-05-07T00:00:00Z","project":[{"_id":"253B6E48-B435-11E9-9278-68D0E5697425","grant_number":"P29638","name":"Drosophila TNFa´s Funktion in Immunzellen","call_identifier":"FWF"},{"_id":"2536F660-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"334077","name":"Investigating the role of transporters in invasive migration through junctions"}],"publication":"Developmental Cell","status":"public","year":"2018","isi":1,"external_id":{"isi":["000432461400009"],"pmid":["29738712"]},"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/"}]},"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2018.04.002","open_access":"1"}],"quality_controlled":"1","page":"331 - 346","date_updated":"2023-09-11T13:22:13Z","_id":"308","type":"journal_article","doi":"10.1016/j.devcel.2018.04.002","article_processing_charge":"No","publisher":"Elsevier","issue":"3","citation":{"chicago":"Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano, and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">https://doi.org/10.1016/j.devcel.2018.04.002</a>.","ista":"Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W, György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 45(3), 331–346.","mla":"Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>, vol. 45, no. 3, Elsevier, 2018, pp. 331–46, doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">10.1016/j.devcel.2018.04.002</a>.","apa":"Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G., … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">https://doi.org/10.1016/j.devcel.2018.04.002</a>","ama":"Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>. 2018;45(3):331-346. doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">10.1016/j.devcel.2018.04.002</a>","short":"A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W. Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018) 331–346.","ieee":"A. Ratheesh <i>et al.</i>, “Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration,” <i>Developmental Cell</i>, vol. 45, no. 3. Elsevier, pp. 331–346, 2018."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"language":[{"iso":"eng"}],"department":[{"_id":"DaSi"},{"_id":"CaHe"},{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"MiSi"}],"month":"05","publication_status":"published","abstract":[{"lang":"eng","text":"Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo."}],"intvolume":"        45","acknowledged_ssus":[{"_id":"SSU"}],"volume":45,"article_type":"original","date_created":"2018-12-11T11:45:44Z","author":[{"first_name":"Aparna","orcid":"0000-0001-7190-0776","full_name":"Ratheesh, Aparna","id":"2F064CFE-F248-11E8-B48F-1D18A9856A87","last_name":"Ratheesh"},{"last_name":"Biebl","full_name":"Biebl, Julia","id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","first_name":"Julia"},{"first_name":"Michael","full_name":"Smutny, Michael","last_name":"Smutny"},{"first_name":"Jana","full_name":"Veselá, Jana","id":"433253EE-F248-11E8-B48F-1D18A9856A87","last_name":"Veselá"},{"full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","last_name":"Papusheva","first_name":"Ekaterina"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kaufmann","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","orcid":"0000-0001-9735-5315"},{"last_name":"György","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87","full_name":"György, Attila","first_name":"Attila","orcid":"0000-0002-1819-198X"},{"last_name":"Casano","id":"3DBA3F4E-F248-11E8-B48F-1D18A9856A87","full_name":"Casano, Alessandra M","orcid":"0000-0002-6009-6804","first_name":"Alessandra M"},{"first_name":"Daria E","orcid":"0000-0001-8323-8353","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","full_name":"Siekhaus, Daria E","last_name":"Siekhaus"}],"day":"07","scopus_import":"1","title":"Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration","oa_version":"Published Version"},{"acknowledged_ssus":[{"_id":"SSU"}],"abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"intvolume":"        19","publication_identifier":{"issn":["14657392"]},"publication_status":"published","title":"Friction forces position the neural anlage","oa_version":"Submitted Version","scopus_import":1,"day":"27","author":[{"orcid":"0000-0002-5920-9090","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael","last_name":"Smutny"},{"full_name":"Ákos, Zsuzsa","last_name":"Ákos","first_name":"Zsuzsa"},{"first_name":"Silvia","last_name":"Grigolon","full_name":"Grigolon, Silvia"},{"last_name":"Shamipour","full_name":"Shamipour, Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan"},{"last_name":"Ruprecht","full_name":"Ruprecht, Verena","first_name":"Verena"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","last_name":"Capek","first_name":"Daniel","orcid":"0000-0001-5199-9940"},{"full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin"},{"last_name":"Papusheva","full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina"},{"first_name":"Masazumi","full_name":"Tada, Masazumi","last_name":"Tada"},{"last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","full_name":"Hof, Björn","first_name":"Björn","orcid":"0000-0003-2057-2754"},{"first_name":"Tamás","full_name":"Vicsek, Tamás","last_name":"Vicsek"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"date_created":"2018-12-11T11:47:46Z","volume":19,"language":[{"iso":"eng"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>.","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>","ieee":"M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317."},"month":"03","department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"page":"306 - 317","quality_controlled":"1","main_file_link":[{"url":"https://europepmc.org/articles/pmc5635970","open_access":"1"}],"publisher":"Nature Publishing Group","doi":"10.1038/ncb3492","type":"journal_article","_id":"661","date_updated":"2024-03-25T23:30:21Z","status":"public","publication":"Nature Cell Biology","project":[{"_id":"25152F3A-B435-11E9-9278-68D0E5697425","grant_number":"306589","name":"Decoding the complexity of turbulence at its origin","call_identifier":"FP7"},{"name":"Control of Epithelial Cell Layer Spreading in Zebrafish","grant_number":"I 930-B20","call_identifier":"FWF","_id":"252ABD0A-B435-11E9-9278-68D0E5697425"}],"date_published":"2017-03-27T00:00:00Z","ec_funded":1,"pmid":1,"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"50"},{"status":"public","relation":"dissertation_contains","id":"8350"}]},"external_id":{"pmid":["28346437"]},"year":"2017","publist_id":"7074"},{"quality_controlled":"1","ddc":["570"],"page":"775 - 788","type":"journal_article","_id":"2022","date_updated":"2021-01-12T06:54:47Z","publisher":"Cell Press","doi":"10.1016/j.cell.2014.10.027","date_published":"2014-11-06T00:00:00Z","ec_funded":1,"publication":"Cell","status":"public","project":[{"name":"Molecular Mechanisms of Cerebral Cortex Development","grant_number":"618444","call_identifier":"FP7","_id":"25D61E48-B435-11E9-9278-68D0E5697425"},{"grant_number":"RGP0053/2014","name":"Quantitative Structure-Function Analysis of Cerebral Cortex Assembly at Clonal Level","_id":"25D7962E-B435-11E9-9278-68D0E5697425"}],"publist_id":"5050","year":"2014","file_date_updated":"2020-07-14T12:45:25Z","publication_status":"published","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"Radial glial progenitors (RGPs) are responsible for producing nearly all neocortical neurons. To gain insight into the patterns of RGP division and neuron production, we quantitatively analyzed excitatory neuron genesis in the mouse neocortex using Mosaic Analysis with Double Markers, which provides single-cell resolution of progenitor division patterns and potential in vivo. We found that RGPs progress through a coherent program in which their proliferative potential diminishes in a predictable manner. Upon entry into the neurogenic phase, individual RGPs produce ∼8–9 neurons distributed in both deep and superficial layers, indicating a unitary output in neuronal production. Removal of OTX1, a transcription factor transiently expressed in RGPs, results in both deep- and superficial-layer neuron loss and a reduction in neuronal unit size. Moreover, ∼1/6 of neurogenic RGPs proceed to produce glia. These results suggest that progenitor behavior and histogenesis in the mammalian neocortex conform to a remarkably orderly and deterministic program.","lang":"eng"}],"intvolume":"       159","has_accepted_license":"1","date_created":"2018-12-11T11:55:16Z","volume":159,"oa_version":"Published Version","title":"Deterministic progenitor behavior and unitary production of neurons in the neocortex","day":"06","scopus_import":1,"author":[{"first_name":"Peng","full_name":"Gao, Peng","last_name":"Gao"},{"last_name":"Postiglione","full_name":"Postiglione, Maria P","id":"2C67902A-F248-11E8-B48F-1D18A9856A87","first_name":"Maria P"},{"last_name":"Krieger","full_name":"Krieger, Teresa","first_name":"Teresa"},{"last_name":"Hernandez","full_name":"Hernandez, Luisirene","first_name":"Luisirene"},{"last_name":"Wang","full_name":"Wang, Chao","first_name":"Chao"},{"first_name":"Zhi","last_name":"Han","full_name":"Han, Zhi"},{"last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","first_name":"Carmen"},{"id":"41DB591E-F248-11E8-B48F-1D18A9856A87","full_name":"Papusheva, Ekaterina","last_name":"Papusheva","first_name":"Ekaterina"},{"last_name":"Insolera","full_name":"Insolera, Ryan","first_name":"Ryan"},{"full_name":"Chugh, Kritika","last_name":"Chugh","first_name":"Kritika"},{"first_name":"Oren","last_name":"Kodish","full_name":"Kodish, Oren"},{"last_name":"Huang","full_name":"Huang, Kun","first_name":"Kun"},{"first_name":"Benjamin","last_name":"Simons","full_name":"Simons, Benjamin"},{"full_name":"Luo, Liqun","last_name":"Luo","first_name":"Liqun"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","first_name":"Simon"},{"first_name":"Song","last_name":"Shi","full_name":"Shi, Song"}],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Gao P, Postiglione MP, Krieger T, Hernandez L, Wang C, Han Z, Streicher C, Papusheva E, Insolera R, Chugh K, Kodish O, Huang K, Simons B, Luo L, Hippenmeyer S, Shi S. 2014. Deterministic progenitor behavior and unitary production of neurons in the neocortex. Cell. 159(4), 775–788.","chicago":"Gao, Peng, Maria P Postiglione, Teresa Krieger, Luisirene Hernandez, Chao Wang, Zhi Han, Carmen Streicher, et al. “Deterministic Progenitor Behavior and Unitary Production of Neurons in the Neocortex.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.10.027\">https://doi.org/10.1016/j.cell.2014.10.027</a>.","apa":"Gao, P., Postiglione, M. P., Krieger, T., Hernandez, L., Wang, C., Han, Z., … Shi, S. (2014). Deterministic progenitor behavior and unitary production of neurons in the neocortex. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.10.027\">https://doi.org/10.1016/j.cell.2014.10.027</a>","mla":"Gao, Peng, et al. “Deterministic Progenitor Behavior and Unitary Production of Neurons in the Neocortex.” <i>Cell</i>, vol. 159, no. 4, Cell Press, 2014, pp. 775–88, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.10.027\">10.1016/j.cell.2014.10.027</a>.","ama":"Gao P, Postiglione MP, Krieger T, et al. Deterministic progenitor behavior and unitary production of neurons in the neocortex. <i>Cell</i>. 2014;159(4):775-788. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.10.027\">10.1016/j.cell.2014.10.027</a>","ieee":"P. Gao <i>et al.</i>, “Deterministic progenitor behavior and unitary production of neurons in the neocortex,” <i>Cell</i>, vol. 159, no. 4. Cell Press, pp. 775–788, 2014.","short":"P. Gao, M.P. Postiglione, T. Krieger, L. Hernandez, C. Wang, Z. Han, C. Streicher, E. Papusheva, R. Insolera, K. Chugh, O. Kodish, K. Huang, B. Simons, L. Luo, S. Hippenmeyer, S. Shi, Cell 159 (2014) 775–788."},"issue":"4","language":[{"iso":"eng"}],"pubrep_id":"423","oa":1,"file":[{"file_id":"4709","date_updated":"2020-07-14T12:45:25Z","creator":"system","date_created":"2018-12-12T10:08:47Z","file_size":4435787,"checksum":"6c5de8329bb2ffa71cba9fda750f14ce","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2016-423-v1+1_1-s2.0-S0092867414013154-main.pdf"}],"department":[{"_id":"SiHi"},{"_id":"Bio"}],"month":"11"},{"publist_id":"1962","year":"2010","external_id":{"pmid":["20717145"]},"pmid":1,"date_published":"2010-08-18T00:00:00Z","status":"public","publication":"EMBO Journal","_id":"4157","date_updated":"2021-01-12T07:54:55Z","type":"journal_article","doi":"10.1038/emboj.2010.182","publisher":"Wiley-Blackwell","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/"}],"quality_controlled":"1","page":"2753 - 2768","department":[{"_id":"Bio"},{"_id":"CaHe"}],"month":"08","citation":{"short":"E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.","ieee":"E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis,” <i>EMBO Journal</i>, vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010.","ama":"Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. 2010;29(16):2753-2768. doi:<a href=\"https://doi.org/10.1038/emboj.2010.182\">10.1038/emboj.2010.182</a>","mla":"Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” <i>EMBO Journal</i>, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:<a href=\"https://doi.org/10.1038/emboj.2010.182\">10.1038/emboj.2010.182</a>.","apa":"Papusheva, E., &#38; Heisenberg, C.-P. J. (2010). Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2010.182\">https://doi.org/10.1038/emboj.2010.182</a>","ista":"Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.","chicago":"Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” <i>EMBO Journal</i>. Wiley-Blackwell, 2010. <a href=\"https://doi.org/10.1038/emboj.2010.182\">https://doi.org/10.1038/emboj.2010.182</a>."},"issue":"16","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"language":[{"iso":"eng"}],"volume":29,"date_created":"2018-12-11T12:07:17Z","scopus_import":1,"day":"18","author":[{"last_name":"Papusheva","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","full_name":"Papusheva, Ekaterina","first_name":"Ekaterina"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"Submitted Version","title":"Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis","publication_status":"published","acknowledged_ssus":[{"_id":"Bio"}],"intvolume":"        29","abstract":[{"lang":"eng","text":"Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis, allowing cells and tissues to change shape without loosing integrity. Studies predominantly in cell culture showed that mechanosensation through adhesion structures is achieved by force-mediated modulation of their molecular composition. The specific molecular composition of adhesion sites in turn determines their signalling activity and dynamic reorganization. Here, we will review how adhesion sites respond to mecanical stimuli, and how spatially and temporally regulated signalling from different adhesion sites controls cell migration and tissue morphogenesis."}]},{"type":"journal_article","date_created":"2018-12-11T12:07:28Z","date_updated":"2021-01-12T07:55:09Z","volume":12,"_id":"4187","title":"A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo","publisher":"Nature Publishing Group","author":[{"first_name":"Elena","full_name":"Kardash, Elena","last_name":"Kardash"},{"first_name":"Michal","full_name":"Reichman-Fried, Michal","last_name":"Reichman Fried"},{"first_name":"Jean","full_name":"Maître, Jean-Léon","last_name":"Maître"},{"first_name":"Bijan","full_name":"Boldajipour, Bijan","last_name":"Boldajipour"},{"first_name":"Ekaterina","last_name":"Papusheva","full_name":"Ekaterina Papusheva","id":"41DB591E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Esther","full_name":"Messerschmidt, Esther-Maria","last_name":"Messerschmidt"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp","first_name":"Carl"},{"last_name":"Raz","full_name":"Raz, Erez","first_name":"Erez"}],"doi":"10.1038/ncb2003","day":"01","quality_controlled":0,"publication_status":"published","intvolume":"        12","abstract":[{"text":"Cell migration is central to embryonic development, homeostasis and disease(1), processes in which cells move as part of a group or individually. Whereas the mechanisms controlling single-cell migration in vitro are relatively well understood(2-4), less is known about the mechanisms promoting the motility of individual cells in vivo. In particular, it is not clear how cells that form blebs in their migration use those protrusions to bring about movement in the context of the three-dimensional cellular environment(5,6). Here we show that the motility of chemokine-guided germ cells within the zebrafish embryo requires the function of the small Rho GTPases Rac1 and RhoA, as well as E-cadherin-mediated cell-cell adhesion. Using fluorescence resonance energy transfer we demonstrate that Rac1 and RhoA are activated in the cell front. At this location, Rac1 is responsible for the formation of actin-rich structures, and RhoA promotes retrograde actin flow. We propose that these actin-rich structures undergoing retrograde flow are essential for the generation of E-cadherin-mediated traction forces between the germ cells and the surrounding tissue and are therefore crucial for cell motility in vivo.","lang":"eng"}],"page":"47 - 53","publist_id":"1932","month":"01","year":"2010","date_published":"2010-01-01T00:00:00Z","issue":"1","citation":{"apa":"Kardash, E., Reichman Fried, M., Maître, J., Boldajipour, B., Papusheva, E., Messerschmidt, E., … Raz, E. (2010). A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2003\">https://doi.org/10.1038/ncb2003</a>","mla":"Kardash, Elena, et al. “A Role for Rho GTPases and Cell-Cell Adhesion in Single-Cell Motility in Vivo.” <i>Nature Cell Biology</i>, vol. 12, no. 1, Nature Publishing Group, 2010, pp. 47–53, doi:<a href=\"https://doi.org/10.1038/ncb2003\">10.1038/ncb2003</a>.","ista":"Kardash E, Reichman Fried M, Maître J, Boldajipour B, Papusheva E, Messerschmidt E, Heisenberg C, Raz E. 2010. A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo. Nature Cell Biology. 12(1), 47–53.","chicago":"Kardash, Elena, Michal Reichman Fried, Jean Maître, Bijan Boldajipour, Ekaterina Papusheva, Esther Messerschmidt, Carl Heisenberg, and Erez Raz. “A Role for Rho GTPases and Cell-Cell Adhesion in Single-Cell Motility in Vivo.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2010. <a href=\"https://doi.org/10.1038/ncb2003\">https://doi.org/10.1038/ncb2003</a>.","ieee":"E. Kardash <i>et al.</i>, “A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo,” <i>Nature Cell Biology</i>, vol. 12, no. 1. Nature Publishing Group, pp. 47–53, 2010.","short":"E. Kardash, M. Reichman Fried, J. Maître, B. Boldajipour, E. Papusheva, E. Messerschmidt, C. Heisenberg, E. Raz, Nature Cell Biology 12 (2010) 47–53.","ama":"Kardash E, Reichman Fried M, Maître J, et al. A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo. <i>Nature Cell Biology</i>. 2010;12(1):47-53. doi:<a href=\"https://doi.org/10.1038/ncb2003\">10.1038/ncb2003</a>"},"extern":1,"publication":"Nature Cell Biology","status":"public"}]