---
_id: '9363'
abstract:
- lang: eng
  text: Optogenetics has been harnessed to shed new mechanistic light on current and
    future therapeutic strategies. This has been to date achieved by the regulation
    of ion flow and electrical signals in neuronal cells and neural circuits that
    are known to be affected by disease. In contrast, the optogenetic delivery of
    trophic biochemical signals, which support cell survival and are implicated in
    degenerative disorders, has never been demonstrated in an animal model of disease.
    Here, we reengineered the human and Drosophila melanogaster REarranged during
    Transfection (hRET and dRET) receptors to be activated by light, creating one-component
    optogenetic tools termed Opto-hRET and Opto-dRET. Upon blue light stimulation,
    these receptors robustly induced the MAPK/ERK proliferative signaling pathway
    in cultured cells. In PINK1B9 flies that exhibit loss of PTEN-induced putative
    kinase 1 (PINK1), a kinase associated with familial Parkinson’s disease (PD),
    light activation of Opto-dRET suppressed mitochondrial defects, tissue degeneration
    and behavioral deficits. In human cells with PINK1 loss-of-function, mitochondrial
    fragmentation was rescued using Opto-dRET via the PI3K/NF-кB pathway. Our results
    demonstrate that a light-activated receptor can ameliorate disease hallmarks in
    a genetic model of PD. The optogenetic delivery of trophic signals is cell type-specific
    and reversible and thus has the potential to inspire novel strategies towards
    a spatio-temporal regulation of tissue repair.
acknowledgement: We thank R. Cagan, A. Whitworth and J. Nagpal for fly lines and advice,
  S. Herlitze for provision of a tissue culture illuminator, and Verian Bader for
  help with statistical analysis.
article_processing_charge: No
author:
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Nikolas
  full_name: Furthmann, Nikolas
  last_name: Furthmann
- first_name: Samuel H.
  full_name: Crossman, Samuel H.
  last_name: Crossman
- first_name: Alexandra Madelaine
  full_name: Tichy, Alexandra Madelaine
  last_name: Tichy
- first_name: Nina
  full_name: Hoyer, Nina
  last_name: Hoyer
- first_name: Meike
  full_name: Petersen, Meike
  last_name: Petersen
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
- first_name: Julia
  full_name: Bicher, Julia
  id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
  last_name: Bicher
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Attila
  full_name: György, Attila
  id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
  last_name: György
  orcid: 0000-0002-1819-198X
- first_name: Daria E
  full_name: Siekhaus, Daria E
  id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
  last_name: Siekhaus
  orcid: 0000-0001-8323-8353
- first_name: Peter
  full_name: Soba, Peter
  last_name: Soba
- first_name: Konstanze F.
  full_name: Winklhofer, Konstanze F.
  last_name: Winklhofer
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Inglés Prieto Á, Furthmann N, Crossman SH, et al. Optogenetic delivery of trophic
    signals in a genetic model of Parkinson’s disease. <i>PLoS genetics</i>. 2021;17(4):e1009479.
    doi:<a href="https://doi.org/10.1371/journal.pgen.1009479">10.1371/journal.pgen.1009479</a>
  apa: Inglés Prieto, Á., Furthmann, N., Crossman, S. H., Tichy, A. M., Hoyer, N.,
    Petersen, M., … Janovjak, H. L. (2021). Optogenetic delivery of trophic signals
    in a genetic model of Parkinson’s disease. <i>PLoS Genetics</i>. Public Library
    of Science. <a href="https://doi.org/10.1371/journal.pgen.1009479">https://doi.org/10.1371/journal.pgen.1009479</a>
  chicago: Inglés Prieto, Álvaro, Nikolas Furthmann, Samuel H. Crossman, Alexandra
    Madelaine Tichy, Nina Hoyer, Meike Petersen, Vanessa Zheden, et al. “Optogenetic
    Delivery of Trophic Signals in a Genetic Model of Parkinson’s Disease.” <i>PLoS
    Genetics</i>. Public Library of Science, 2021. <a href="https://doi.org/10.1371/journal.pgen.1009479">https://doi.org/10.1371/journal.pgen.1009479</a>.
  ieee: Á. Inglés Prieto <i>et al.</i>, “Optogenetic delivery of trophic signals in
    a genetic model of Parkinson’s disease,” <i>PLoS genetics</i>, vol. 17, no. 4.
    Public Library of Science, p. e1009479, 2021.
  ista: Inglés Prieto Á, Furthmann N, Crossman SH, Tichy AM, Hoyer N, Petersen M,
    Zheden V, Bicher J, Gschaider-Reichhart E, György A, Siekhaus DE, Soba P, Winklhofer
    KF, Janovjak HL. 2021. Optogenetic delivery of trophic signals in a genetic model
    of Parkinson’s disease. PLoS genetics. 17(4), e1009479.
  mla: Inglés Prieto, Álvaro, et al. “Optogenetic Delivery of Trophic Signals in a
    Genetic Model of Parkinson’s Disease.” <i>PLoS Genetics</i>, vol. 17, no. 4, Public
    Library of Science, 2021, p. e1009479, doi:<a href="https://doi.org/10.1371/journal.pgen.1009479">10.1371/journal.pgen.1009479</a>.
  short: Á. Inglés Prieto, N. Furthmann, S.H. Crossman, A.M. Tichy, N. Hoyer, M. Petersen,
    V. Zheden, J. Bicher, E. Gschaider-Reichhart, A. György, D.E. Siekhaus, P. Soba,
    K.F. Winklhofer, H.L. Janovjak, PLoS Genetics 17 (2021) e1009479.
date_created: 2021-05-02T22:01:29Z
date_published: 2021-04-01T00:00:00Z
date_updated: 2023-08-08T13:17:47Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: LoSw
- _id: DaSi
doi: 10.1371/journal.pgen.1009479
external_id:
  isi:
  - '000640606700001'
file:
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issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: e1009479
publication: PLoS genetics
publication_identifier:
  eissn:
  - '15537404'
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 17
year: '2021'
...
---
_id: '418'
abstract:
- lang: eng
  text: "The aim of this thesis was the development of new strategies for optical
    and optogenetic control of proliferative and pro-survival signaling, and characterizing
    them from the molecular mechanism up to cellular effects. These new light-based
    methods have unique features, such as red light as an activator, or the avoidance
    of gene delivery, which enable to overcome current limitations, such as light
    delivery to target tissues and feasibility as therapeutic approach. A special
    focus was placed on implementing these new light-based approaches in pancreatic
    β-cells, as β-cells are the key players in diabetes and especially their loss
    in number negatively affects disease progression. Currently no treatment options
    are available to compensate the lack of functional β-cells in diabetic patients.\r\nIn
    a first approach, red-light-activated growth factor receptors, in particular receptor
    tyrosine kinases were engineered and characterized. Receptor activation with light
    allows spatio-temporal control compared to ligand-based activation, and especially
    red light exhibits deeper tissue penetration than other wavelengths of the visible
    spectrum. Red-light-activated receptor tyrosine kinases robustly activated major
    growth factor related signaling pathways with a high temporal resolution. Moreover,
    the remote activation of the proliferative MAPK/Erk pathway by red-light-activated
    receptor tyrosine kinases in a pancreatic β-cell line was also achieved, through
    one centimeter thick mouse tissue. Although red-light-activated receptor tyrosine
    kinases are particularly attractive for applications in animal models due to the
    deep tissue penetration of red light, a drawback, especially with regard to translation
    into humans, is the requirement of gene therapy.\r\nIn a second approach an endogenous
    light-sensitive mechanism was identified and its potential to promote proliferative
    and pro-survival signals was explored, towards light-based tissue regeneration
    without the need for gene transfer. Blue-green light illumination was found to
    be sufficient for the activation of proliferation and survival promoting signaling
    pathways in primary pancreatic murine and human islets. Blue-green light also
    led to an increase in proliferation of primary islet cells, an effect which was
    shown to be mostly β-cell specific in human islets. Moreover, it was demonstrated
    that this approach of pancreatic β-cell expansion did not have any negative effect
    on the β-cell function, in particular on their insulin secretion capacity. In
    contrast, a trend for enhanced insulin secretion under high glucose conditions
    after illumination was detected. In order to unravel the detailed characteristics
    of this endogenous light-sensitive mechanism, the precise light requirements were
    determined. In addition, the expression of light sensing proteins, OPN3 and rhodopsin,
    was detected. The observed effects were found to be independent of handling effects
    such as temperature differences and cytochrome c oxidase dependent ATP increase,
    but they were found to be enhanced through the knockout of OPN3. The exact mechanism
    of how islets cells sense light and the identity of the photoreceptor remains
    unknown.\r\nSummarized two new light-based systems with unique features were established
    that enable the activation of proliferative and pro-survival signaling pathways.
    While red-light-activated receptor tyrosine kinases open a new avenue for optogenetics
    research, by allowing non-invasive control of signaling in vivo, the identified
    endogenous light-sensitive mechanism has the potential to be the basis of a gene
    therapy-free therapeutical approach for light-based β-cell expansion."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
citation:
  ama: Gschaider-Reichhart E. Optical and optogenetic control of proliferation and
    survival . 2018. doi:<a href="https://doi.org/10.15479/AT:ISTA:th_913">10.15479/AT:ISTA:th_913</a>
  apa: Gschaider-Reichhart, E. (2018). <i>Optical and optogenetic control of proliferation
    and survival </i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:th_913">https://doi.org/10.15479/AT:ISTA:th_913</a>
  chicago: Gschaider-Reichhart, Eva. “Optical and Optogenetic Control of Proliferation
    and Survival .” Institute of Science and Technology Austria, 2018. <a href="https://doi.org/10.15479/AT:ISTA:th_913">https://doi.org/10.15479/AT:ISTA:th_913</a>.
  ieee: E. Gschaider-Reichhart, “Optical and optogenetic control of proliferation
    and survival ,” Institute of Science and Technology Austria, 2018.
  ista: Gschaider-Reichhart E. 2018. Optical and optogenetic control of proliferation
    and survival . Institute of Science and Technology Austria.
  mla: Gschaider-Reichhart, Eva. <i>Optical and Optogenetic Control of Proliferation
    and Survival </i>. Institute of Science and Technology Austria, 2018, doi:<a href="https://doi.org/10.15479/AT:ISTA:th_913">10.15479/AT:ISTA:th_913</a>.
  short: E. Gschaider-Reichhart, Optical and Optogenetic Control of Proliferation
    and Survival , Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:46:22Z
date_published: 2018-01-08T00:00:00Z
date_updated: 2023-09-22T09:20:10Z
day: '08'
ddc:
- '571'
- '570'
degree_awarded: PhD
department:
- _id: HaJa
doi: 10.15479/AT:ISTA:th_913
file:
- access_level: closed
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file_date_updated: 2020-07-14T12:46:24Z
has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: '107'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7405'
pubrep_id: '913'
related_material:
  record:
  - id: '1441'
    relation: part_of_dissertation
    status: public
  - id: '1678'
    relation: part_of_dissertation
    status: public
  - id: '2084'
    relation: part_of_dissertation
    status: public
  - id: '1028'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
title: 'Optical and optogenetic control of proliferation and survival '
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '538'
abstract:
- lang: ger
  text: 'Optogenetik und Photopharmakologie ermöglichen präzise räumliche und zeitliche
    Kontrolle von Proteinwechselwirkung und -funktion in Zellen und Tieren. Optogenetische
    Methoden, die auf grünes Licht ansprechen und zum Trennen von Proteinkomplexen
    geeignet sind, sind nichtweitläufig verfügbar, würden jedoch mehrfarbige Experimente
    zur Beantwortung von biologischen Fragestellungen ermöglichen. Hier demonstrieren
    wir die Verwendung von Cobalamin(Vitamin B12)-bindenden Domänen von bakteriellen
    CarH-Transkriptionsfaktoren zur Grünlicht-induzierten Dissoziation von Rezeptoren.
    Fusioniert mit dem Fibroblasten-W achstumsfaktor-Rezeptor 1 führten diese im Dunkeln
    in kultivierten Zellen zu Signalaktivität durch Oligomerisierung, welche durch
    Beleuchten umgehend aufgehoben wurde. In Zebrafischembryonen, die einen derartigen
    Rezeptor exprimieren, ermöglichte grünes Licht die Kontrolle über abnormale Signalaktivität
    während der Embryonalentwicklung. '
author:
- first_name: Stephanie
  full_name: Kainrath, Stephanie
  id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
  last_name: Kainrath
- first_name: Manuela
  full_name: Stadler, Manuela
  last_name: Stadler
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Martin
  full_name: Distel, Martin
  last_name: Distel
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. Grünlicht-induzierte
    Rezeptorinaktivierung durch Cobalamin-bindende Domänen. <i>Angewandte Chemie</i>.
    2017;129(16):4679-4682. doi:<a href="https://doi.org/10.1002/ange.201611998">10.1002/ange.201611998</a>
  apa: Kainrath, S., Stadler, M., Gschaider-Reichhart, E., Distel, M., &#38; Janovjak,
    H. L. (2017). Grünlicht-induzierte Rezeptorinaktivierung durch Cobalamin-bindende
    Domänen. <i>Angewandte Chemie</i>. Wiley. <a href="https://doi.org/10.1002/ange.201611998">https://doi.org/10.1002/ange.201611998</a>
  chicago: Kainrath, Stephanie, Manuela Stadler, Eva Gschaider-Reichhart, Martin Distel,
    and Harald L Janovjak. “Grünlicht-Induzierte Rezeptorinaktivierung Durch Cobalamin-Bindende
    Domänen.” <i>Angewandte Chemie</i>. Wiley, 2017. <a href="https://doi.org/10.1002/ange.201611998">https://doi.org/10.1002/ange.201611998</a>.
  ieee: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, and H. L. Janovjak,
    “Grünlicht-induzierte Rezeptorinaktivierung durch Cobalamin-bindende Domänen,”
    <i>Angewandte Chemie</i>, vol. 129, no. 16. Wiley, pp. 4679–4682, 2017.
  ista: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. 2017.
    Grünlicht-induzierte Rezeptorinaktivierung durch Cobalamin-bindende Domänen. Angewandte
    Chemie. 129(16), 4679–4682.
  mla: Kainrath, Stephanie, et al. “Grünlicht-Induzierte Rezeptorinaktivierung Durch
    Cobalamin-Bindende Domänen.” <i>Angewandte Chemie</i>, vol. 129, no. 16, Wiley,
    2017, pp. 4679–82, doi:<a href="https://doi.org/10.1002/ange.201611998">10.1002/ange.201611998</a>.
  short: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, H.L. Janovjak,
    Angewandte Chemie 129 (2017) 4679–4682.
date_created: 2018-12-11T11:47:02Z
date_published: 2017-05-20T00:00:00Z
date_updated: 2021-01-12T08:01:33Z
day: '20'
ddc:
- '571'
department:
- _id: CaGu
- _id: HaJa
doi: 10.1002/ange.201611998
ec_funded: 1
file:
- access_level: open_access
  checksum: d66fee867e7cdbfa3fe276c2fb0778bb
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:24Z
  date_updated: 2020-07-14T12:46:39Z
  file_id: '5007'
  file_name: IST-2018-932-v1+1_Kainrath_et_al-2017-Angewandte_Chemie.pdf
  file_size: 1668557
  relation: main_file
file_date_updated: 2020-07-14T12:46:39Z
has_accepted_license: '1'
intvolume: '       129'
issue: '16'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 4679 - 4682
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 255A6082-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W1232-B24
  name: Molecular Drug Targets
publication: Angewandte Chemie
publication_status: published
publisher: Wiley
publist_id: '7279'
pubrep_id: '932'
quality_controlled: '1'
status: public
title: Grünlicht-induzierte Rezeptorinaktivierung durch Cobalamin-bindende Domänen
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 129
year: '2017'
...
---
_id: '1028'
abstract:
- lang: eng
  text: Optogenetics and photopharmacology provide spatiotemporally precise control
    over protein interactions and protein function in cells and animals. Optogenetic
    methods that are sensitive to green light and can be used to break protein complexes
    are not broadly available but would enable multichromatic experiments with previously
    inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12)
    binding domains of bacterial CarH transcription factors for green-light-induced
    receptor dissociation. In cultured cells, we observed oligomerization-induced
    cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin-binding
    domains in the dark that was rapidly eliminated upon illumination. In zebrafish
    embryos expressing fusion receptors, green light endowed control over aberrant
    fibroblast growth factor signaling during development. Green-light-induced domain
    dissociation and light-inactivated receptors will critically expand the optogenetic
    toolbox for control of biological processes.
acknowledgement: "This work was supported by a grant from the European Union\U0010FC1Ds
  Seventh Framework Programme (CIG-303564). E.R. was supported by the graduate program
  MolecularDrugTargets (Austrian Science Fund (FWF), W1232) and a FemTech fellowship
  (Austrian Research Promotion Agency, 3580812)"
article_processing_charge: No
author:
- first_name: Stephanie
  full_name: Kainrath, Stephanie
  id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
  last_name: Kainrath
- first_name: Manuela
  full_name: Stadler, Manuela
  last_name: Stadler
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Martin
  full_name: Distel, Martin
  last_name: Distel
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. Green-light-induced
    inactivation of receptor signaling using cobalamin-binding domains. <i>Angewandte
    Chemie - International Edition</i>. 2017;56(16):4608-4611. doi:<a href="https://doi.org/10.1002/anie.201611998">10.1002/anie.201611998</a>
  apa: Kainrath, S., Stadler, M., Gschaider-Reichhart, E., Distel, M., &#38; Janovjak,
    H. L. (2017). Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains. <i>Angewandte Chemie - International Edition</i>. Wiley-Blackwell. <a
    href="https://doi.org/10.1002/anie.201611998">https://doi.org/10.1002/anie.201611998</a>
  chicago: Kainrath, Stephanie, Manuela Stadler, Eva Gschaider-Reichhart, Martin Distel,
    and Harald L Janovjak. “Green-Light-Induced Inactivation of Receptor Signaling
    Using Cobalamin-Binding Domains.” <i>Angewandte Chemie - International Edition</i>.
    Wiley-Blackwell, 2017. <a href="https://doi.org/10.1002/anie.201611998">https://doi.org/10.1002/anie.201611998</a>.
  ieee: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, and H. L. Janovjak,
    “Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains,” <i>Angewandte Chemie - International Edition</i>, vol. 56, no. 16. Wiley-Blackwell,
    pp. 4608–4611, 2017.
  ista: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. 2017.
    Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains. Angewandte Chemie - International Edition. 56(16), 4608–4611.
  mla: Kainrath, Stephanie, et al. “Green-Light-Induced Inactivation of Receptor Signaling
    Using Cobalamin-Binding Domains.” <i>Angewandte Chemie - International Edition</i>,
    vol. 56, no. 16, Wiley-Blackwell, 2017, pp. 4608–11, doi:<a href="https://doi.org/10.1002/anie.201611998">10.1002/anie.201611998</a>.
  short: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, H.L. Janovjak,
    Angewandte Chemie - International Edition 56 (2017) 4608–4611.
date_created: 2018-12-11T11:49:46Z
date_published: 2017-03-20T00:00:00Z
date_updated: 2024-03-25T23:30:08Z
day: '20'
ddc:
- '540'
department:
- _id: CaGu
- _id: HaJa
doi: 10.1002/anie.201611998
ec_funded: 1
external_id:
  isi:
  - '000398154000038'
file:
- access_level: open_access
  content_type: application/pdf
  creator: dernst
  date_created: 2019-01-18T09:39:55Z
  date_updated: 2019-01-18T09:39:55Z
  file_id: '5845'
  file_name: 2017_communications_Kainrath.pdf
  file_size: 2614942
  relation: main_file
  success: 1
file_date_updated: 2019-01-18T09:39:55Z
has_accepted_license: '1'
intvolume: '        56'
isi: 1
issue: '16'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 4608-4611
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W1232-B24
  name: Molecular Drug Targets [do not use to be deleted]
publication: Angewandte Chemie - International Edition
publication_identifier:
  issn:
  - '14337851'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6362'
quality_controlled: '1'
related_material:
  record:
  - id: '418'
    relation: dissertation_contains
    status: public
  - id: '7680'
    relation: part_of_dissertation
    status: public
scopus_import: '1'
status: public
title: Green-light-induced inactivation of receptor signaling using cobalamin-binding
  domains
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 56
year: '2017'
...
---
_id: '1441'
abstract:
- lang: eng
  text: 'Optogenetics and photopharmacology enable the spatio-temporal control of
    cell and animal behavior by light. Although red light offers deep-tissue penetration
    and minimal phototoxicity, very few red-light-sensitive optogenetic methods are
    currently available. We have now developed a red-light-induced homodimerization
    domain. We first showed that an optimized sensory domain of the cyanobacterial
    phytochrome 1 can be expressed robustly and without cytotoxicity in human cells.
    We then applied this domain to induce the dimerization of two receptor tyrosine
    kinases—the fibroblast growth factor receptor 1 and the neurotrophin receptor
    trkB. This new optogenetic method was then used to activate the MAPK/ERK pathway
    non-invasively in mammalian tissue and in multicolor cell-signaling experiments.
    The light-controlled dimerizer and red-light-activated receptor tyrosine kinases
    will prove useful to regulate a variety of cellular processes with light. Go deep
    with red: The sensory domain (S) of the cyanobacterial phytochrome 1 (CPH1) was
    repurposed to induce the homodimerization of proteins in living cells by red light.
    By using this domain, light-activated protein kinases were engineered that can
    be activated orthogonally from many fluorescent proteins and through mammalian
    tissue. Pr/Pfr=red-/far-red-absorbing state of CPH1.'
acknowledgement: 'A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by
  the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W1232) and
  a FemTech fellowship (Austrian Research Promotion Agency: 3580812).'
author:
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Alexandra-Madelaine
  full_name: Tichy, Alexandra-Madelaine
  id: 29D8BB2C-F248-11E8-B48F-1D18A9856A87
  last_name: Tichy
- first_name: Catherine
  full_name: Mckenzie, Catherine
  id: 3EEDE19A-F248-11E8-B48F-1D18A9856A87
  last_name: Mckenzie
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Gschaider-Reichhart E, Inglés Prieto Á, Tichy A-M, Mckenzie C, Janovjak HL.
    A phytochrome sensory domain permits receptor activation by red light. <i>Angewandte
    Chemie - International Edition</i>. 2016;55(21):6339-6342. doi:<a href="https://doi.org/10.1002/anie.201601736">10.1002/anie.201601736</a>
  apa: Gschaider-Reichhart, E., Inglés Prieto, Á., Tichy, A.-M., Mckenzie, C., &#38;
    Janovjak, H. L. (2016). A phytochrome sensory domain permits receptor activation
    by red light. <i>Angewandte Chemie - International Edition</i>. Wiley. <a href="https://doi.org/10.1002/anie.201601736">https://doi.org/10.1002/anie.201601736</a>
  chicago: Gschaider-Reichhart, Eva, Álvaro Inglés Prieto, Alexandra-Madelaine Tichy,
    Catherine Mckenzie, and Harald L Janovjak. “A Phytochrome Sensory Domain Permits
    Receptor Activation by Red Light.” <i>Angewandte Chemie - International Edition</i>.
    Wiley, 2016. <a href="https://doi.org/10.1002/anie.201601736">https://doi.org/10.1002/anie.201601736</a>.
  ieee: E. Gschaider-Reichhart, Á. Inglés Prieto, A.-M. Tichy, C. Mckenzie, and H.
    L. Janovjak, “A phytochrome sensory domain permits receptor activation by red
    light,” <i>Angewandte Chemie - International Edition</i>, vol. 55, no. 21. Wiley,
    pp. 6339–6342, 2016.
  ista: Gschaider-Reichhart E, Inglés Prieto Á, Tichy A-M, Mckenzie C, Janovjak HL.
    2016. A phytochrome sensory domain permits receptor activation by red light. Angewandte
    Chemie - International Edition. 55(21), 6339–6342.
  mla: Gschaider-Reichhart, Eva, et al. “A Phytochrome Sensory Domain Permits Receptor
    Activation by Red Light.” <i>Angewandte Chemie - International Edition</i>, vol.
    55, no. 21, Wiley, 2016, pp. 6339–42, doi:<a href="https://doi.org/10.1002/anie.201601736">10.1002/anie.201601736</a>.
  short: E. Gschaider-Reichhart, Á. Inglés Prieto, A.-M. Tichy, C. Mckenzie, H.L.
    Janovjak, Angewandte Chemie - International Edition 55 (2016) 6339–6342.
date_created: 2018-12-11T11:52:02Z
date_published: 2016-05-17T00:00:00Z
date_updated: 2023-09-07T12:49:08Z
day: '17'
ddc:
- '571'
- '576'
department:
- _id: HaJa
doi: 10.1002/anie.201601736
ec_funded: 1
file:
- access_level: open_access
  checksum: 26da07960e57ac4750b54179197ce57f
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:17:03Z
  date_updated: 2020-07-14T12:44:55Z
  file_id: '5255'
  file_name: IST-2017-840-v1+1_reichhart.pdf
  file_size: 1268662
  relation: main_file
file_date_updated: 2020-07-14T12:44:55Z
has_accepted_license: '1'
intvolume: '        55'
issue: '21'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 6339 - 6342
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 255A6082-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W1232-B24
  name: Molecular Drug Targets
publication: Angewandte Chemie - International Edition
publication_status: published
publisher: Wiley
publist_id: '5755'
pubrep_id: '840'
quality_controlled: '1'
related_material:
  record:
  - id: '418'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: A phytochrome sensory domain permits receptor activation by red light
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2016'
...
---
_id: '1678'
abstract:
- lang: eng
  text: High-throughput live-cell screens are intricate elements of systems biology
    studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted
    method that avoids the need for chemical activators and reporters, reduces the
    number of operational steps and increases information content in a cell-based
    small-molecule screen against human protein kinases, including an orphan receptor
    tyrosine kinase. This blueprint for all-optical screening can be adapted to many
    drug targets and cellular processes.
acknowledgement: 'This work was supported by grants from the European Union Seventh
  Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human
  Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to
  M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate
  program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech
  fellowship (3580812 Austrian Research Promotion Agency).'
author:
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Markus
  full_name: Muellner, Markus
  last_name: Muellner
- first_name: Matthias
  full_name: Nowak, Matthias
  id: 30845DAA-F248-11E8-B48F-1D18A9856A87
  last_name: Nowak
- first_name: Sebastian
  full_name: Nijman, Sebastian
  last_name: Nijman
- first_name: Michael
  full_name: Grusch, Michael
  last_name: Grusch
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule
    screening against protein kinases. <i>Nature Chemical Biology</i>. 2015;11(12):952-954.
    doi:<a href="https://doi.org/10.1038/nchembio.1933">10.1038/nchembio.1933</a>
  apa: Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman,
    S., Grusch, M., &#38; Janovjak, H. L. (2015). Light-assisted small-molecule screening
    against protein kinases. <i>Nature Chemical Biology</i>. Nature Publishing Group.
    <a href="https://doi.org/10.1038/nchembio.1933">https://doi.org/10.1038/nchembio.1933</a>
  chicago: Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias
    Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted
    Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>.
    Nature Publishing Group, 2015. <a href="https://doi.org/10.1038/nchembio.1933">https://doi.org/10.1038/nchembio.1933</a>.
  ieee: Á. Inglés Prieto <i>et al.</i>, “Light-assisted small-molecule screening against
    protein kinases,” <i>Nature Chemical Biology</i>, vol. 11, no. 12. Nature Publishing
    Group, pp. 952–954, 2015.
  ista: Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch
    M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein
    kinases. Nature Chemical Biology. 11(12), 952–954.
  mla: Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against
    Protein Kinases.” <i>Nature Chemical Biology</i>, vol. 11, no. 12, Nature Publishing
    Group, 2015, pp. 952–54, doi:<a href="https://doi.org/10.1038/nchembio.1933">10.1038/nchembio.1933</a>.
  short: Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman,
    M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954.
date_created: 2018-12-11T11:53:25Z
date_published: 2015-10-12T00:00:00Z
date_updated: 2023-09-07T12:49:09Z
day: '12'
ddc:
- '571'
department:
- _id: HaJa
- _id: LifeSc
doi: 10.1038/nchembio.1933
ec_funded: 1
file:
- access_level: open_access
  checksum: e9fb251dfcb7cd209b83f17867e61321
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:10:51Z
  date_updated: 2020-07-14T12:45:12Z
  file_id: '4842'
  file_name: IST-2017-837-v1+1_ingles-prieto.pdf
  file_size: 1308364
  relation: main_file
file_date_updated: 2020-07-14T12:45:12Z
has_accepted_license: '1'
intvolume: '        11'
issue: '12'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 952 - 954
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 255BFFFA-B435-11E9-9278-68D0E5697425
  grant_number: RGY0084/2012
  name: In situ real-time imaging of neurotransmitter signaling using designer optical
    sensors (HFSP Young Investigator)
- _id: 255A6082-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W1232-B24
  name: Molecular Drug Targets
publication: Nature Chemical Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5471'
pubrep_id: '837'
quality_controlled: '1'
related_material:
  record:
  - id: '418'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Light-assisted small-molecule screening against protein kinases
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2015'
...
---
_id: '2032'
abstract:
- lang: eng
  text: As light-based control of fundamental signaling pathways is becoming a reality,
    the field of optogenetics is rapidly moving beyond neuroscience. We have recently
    developed receptor tyrosine kinases that are activated by light and control cell
    proliferation, epithelial–mesenchymal transition, and angiogenic sprouting—cell
    behaviors central to cancer progression.
article_number: e964045
author:
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Karin
  full_name: Schelch, Karin
  last_name: Schelch
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
- first_name: Michael
  full_name: Grusch, Michael
  last_name: Grusch
citation:
  ama: 'Inglés Prieto Á, Gschaider-Reichhart E, Schelch K, Janovjak HL, Grusch M.
    The optogenetic promise for oncology: Episode I. <i>Molecular and Cellular Oncology</i>.
    2014;1(4). doi:<a href="https://doi.org/10.4161/23723548.2014.964045">10.4161/23723548.2014.964045</a>'
  apa: 'Inglés Prieto, Á., Gschaider-Reichhart, E., Schelch, K., Janovjak, H. L.,
    &#38; Grusch, M. (2014). The optogenetic promise for oncology: Episode I. <i>Molecular
    and Cellular Oncology</i>. Taylor &#38; Francis. <a href="https://doi.org/10.4161/23723548.2014.964045">https://doi.org/10.4161/23723548.2014.964045</a>'
  chicago: 'Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Karin Schelch, Harald
    L Janovjak, and Michael Grusch. “The Optogenetic Promise for Oncology: Episode
    I.” <i>Molecular and Cellular Oncology</i>. Taylor &#38; Francis, 2014. <a href="https://doi.org/10.4161/23723548.2014.964045">https://doi.org/10.4161/23723548.2014.964045</a>.'
  ieee: 'Á. Inglés Prieto, E. Gschaider-Reichhart, K. Schelch, H. L. Janovjak, and
    M. Grusch, “The optogenetic promise for oncology: Episode I,” <i>Molecular and
    Cellular Oncology</i>, vol. 1, no. 4. Taylor &#38; Francis, 2014.'
  ista: 'Inglés Prieto Á, Gschaider-Reichhart E, Schelch K, Janovjak HL, Grusch M.
    2014. The optogenetic promise for oncology: Episode I. Molecular and Cellular
    Oncology. 1(4), e964045.'
  mla: 'Inglés Prieto, Álvaro, et al. “The Optogenetic Promise for Oncology: Episode
    I.” <i>Molecular and Cellular Oncology</i>, vol. 1, no. 4, e964045, Taylor &#38;
    Francis, 2014, doi:<a href="https://doi.org/10.4161/23723548.2014.964045">10.4161/23723548.2014.964045</a>.'
  short: Á. Inglés Prieto, E. Gschaider-Reichhart, K. Schelch, H.L. Janovjak, M. Grusch,
    Molecular and Cellular Oncology 1 (2014).
date_created: 2018-12-11T11:55:19Z
date_published: 2014-12-31T00:00:00Z
date_updated: 2021-01-12T06:54:51Z
day: '31'
ddc:
- '570'
department:
- _id: HaJa
doi: 10.4161/23723548.2014.964045
file:
- access_level: open_access
  checksum: 44e17ad40577ab46eb602e88a8b0b8fd
  content_type: application/pdf
  creator: kschuh
  date_created: 2019-05-16T13:39:11Z
  date_updated: 2020-07-14T12:45:26Z
  file_id: '6464'
  file_name: 2014_Taylor_Alvaro.pdf
  file_size: 1765933
  relation: main_file
file_date_updated: 2020-07-14T12:45:26Z
has_accepted_license: '1'
intvolume: '         1'
issue: '4'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: Molecular and Cellular Oncology
publication_status: published
publisher: Taylor & Francis
publist_id: '5040'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'The optogenetic promise for oncology: Episode I'
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2014'
...
---
_id: '2084'
abstract:
- lang: eng
  text: Receptor tyrosine kinases (RTKs) are a large family of cell surface receptors
    that sense growth factors and hormones and regulate a variety of cell behaviours
    in health and disease. Contactless activation of RTKs with spatial and temporal
    precision is currently not feasible. Here, we generated RTKs that are insensitive
    to endogenous ligands but can be selectively activated by low-intensity blue light.
    We screened light-oxygen-voltage (LOV)-sensing domains for their ability to activate
    RTKs by light-activated dimerization. Incorporation of LOV domains found in aureochrome
    photoreceptors of stramenopiles resulted in robust activation of the fibroblast
    growth factor receptor 1 (FGFR1), epidermal growth factor receptor (EGFR) and
    rearranged during transfection (RET). In human cancer and endothelial cells, light
    induced cellular signalling with spatial and temporal precision. Furthermore,
    light faithfully mimicked complex mitogenic and morphogenic cell behaviour induced
    by growth factors. RTKs under optical control (Opto-RTKs) provide a powerful optogenetic
    approach to actuate cellular signals and manipulate cell behaviour.
acknowledgement: European Union Seventh Framework Programme; Human Frontier Science
  Program; Oesterreichische Nationalbank Anniversary Fund 14211; Austrian Research
  Promotion Agency; FemTech
author:
- first_name: Michael
  full_name: Grusch, Michael
  last_name: Grusch
- first_name: Karin
  full_name: Schelch, Karin
  last_name: Schelch
- first_name: Robert
  full_name: Riedler, Robert
  last_name: Riedler
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Christopher
  full_name: Differ, Christopher
  last_name: Differ
- first_name: Walter
  full_name: Berger, Walter
  last_name: Berger
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Grusch M, Schelch K, Riedler R, et al. Spatio-temporally precise activation
    of engineered receptor tyrosine kinases by light. <i>EMBO Journal</i>. 2014;33(15):1713-1726.
    doi:<a href="https://doi.org/10.15252/embj.201387695">10.15252/embj.201387695</a>
  apa: Grusch, M., Schelch, K., Riedler, R., Gschaider-Reichhart, E., Differ, C.,
    Berger, W., … Janovjak, H. L. (2014). Spatio-temporally precise activation of
    engineered receptor tyrosine kinases by light. <i>EMBO Journal</i>. Wiley-Blackwell.
    <a href="https://doi.org/10.15252/embj.201387695">https://doi.org/10.15252/embj.201387695</a>
  chicago: Grusch, Michael, Karin Schelch, Robert Riedler, Eva Gschaider-Reichhart,
    Christopher Differ, Walter Berger, Álvaro Inglés Prieto, and Harald L Janovjak.
    “Spatio-Temporally Precise Activation of Engineered Receptor Tyrosine Kinases
    by Light.” <i>EMBO Journal</i>. Wiley-Blackwell, 2014. <a href="https://doi.org/10.15252/embj.201387695">https://doi.org/10.15252/embj.201387695</a>.
  ieee: M. Grusch <i>et al.</i>, “Spatio-temporally precise activation of engineered
    receptor tyrosine kinases by light,” <i>EMBO Journal</i>, vol. 33, no. 15. Wiley-Blackwell,
    pp. 1713–1726, 2014.
  ista: Grusch M, Schelch K, Riedler R, Gschaider-Reichhart E, Differ C, Berger W,
    Inglés Prieto Á, Janovjak HL. 2014. Spatio-temporally precise activation of engineered
    receptor tyrosine kinases by light. EMBO Journal. 33(15), 1713–1726.
  mla: Grusch, Michael, et al. “Spatio-Temporally Precise Activation of Engineered
    Receptor Tyrosine Kinases by Light.” <i>EMBO Journal</i>, vol. 33, no. 15, Wiley-Blackwell,
    2014, pp. 1713–26, doi:<a href="https://doi.org/10.15252/embj.201387695">10.15252/embj.201387695</a>.
  short: M. Grusch, K. Schelch, R. Riedler, E. Gschaider-Reichhart, C. Differ, W.
    Berger, Á. Inglés Prieto, H.L. Janovjak, EMBO Journal 33 (2014) 1713–1726.
date_created: 2018-12-11T11:55:37Z
date_published: 2014-07-01T00:00:00Z
date_updated: 2023-09-07T12:49:09Z
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doi: 10.15252/embj.201387695
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publication: EMBO Journal
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title: Spatio-temporally precise activation of engineered receptor tyrosine kinases
  by light
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2014'
...