---
_id: '14843'
abstract:
- lang: eng
  text: The coupling between Ca2+ channels and release sensors is a key factor defining
    the signaling properties of a synapse. However, the coupling nanotopography at
    many synapses remains unknown, and it is unclear how it changes during development.
    To address these questions, we examined coupling at the cerebellar inhibitory
    basket cell (BC)-Purkinje cell (PC) synapse. Biophysical analysis of transmission
    by paired recording and intracellular pipette perfusion revealed that the effects
    of exogenous Ca2+ chelators decreased during development, despite constant reliance
    of release on P/Q-type Ca2+ channels. Structural analysis by freeze-fracture replica
    labeling (FRL) and transmission electron microscopy (EM) indicated that presynaptic
    P/Q-type Ca2+ channels formed nanoclusters throughout development, whereas docked
    vesicles were only clustered at later developmental stages. Modeling suggested
    a developmental transformation from a more random to a more clustered coupling
    nanotopography. Thus, presynaptic signaling developmentally approaches a point-to-point
    configuration, optimizing speed, reliability, and energy efficiency of synaptic
    transmission.
acknowledged_ssus:
- _id: EM-Fac
- _id: PreCl
- _id: M-Shop
acknowledgement: We thank Drs. David DiGregorio and Erwin Neher for critically reading
  an earlier version of the manuscript, Ralf Schneggenburger for helpful discussions,
  Benjamin Suter and Katharina Lichter for support with image analysis, Chris Wojtan
  for advice on numerical solution of partial differential equations, Maria Reva for
  help with Ripley analysis, Alois Schlögl for programming, and Akari Hagiwara and
  Toshihisa Ohtsuka for anti-ELKS antibody. We are grateful to Florian Marr, Christina
  Altmutter, and Vanessa Zheden for excellent technical assistance and to Eleftheria
  Kralli-Beller for manuscript editing. This research was supported by the Scientific
  Services Units (SSUs) of ISTA (Electron Microscopy Facility, Preclinical Facility,
  and Machine Shop). The project received funding from the European Research Council
  (ERC) under the European Union’s Horizon 2020 research and innovation program (grant
  agreement no. 692692), the Fonds zur Förderung der Wissenschaftlichen Forschung
  (Z 312-B27, Wittgenstein award; P 36232-B), all to P.J., and a DOC fellowship of
  the Austrian Academy of Sciences to J.-J.C.
article_processing_charge: No
article_type: original
author:
- first_name: JingJing
  full_name: Chen, JingJing
  id: 2C4E65C8-F248-11E8-B48F-1D18A9856A87
  last_name: Chen
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Chong
  full_name: Chen, Chong
  id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
  last_name: Chen
- first_name: Itaru
  full_name: Arai, Itaru
  id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
  last_name: Arai
- first_name: Olena
  full_name: Kim, Olena
  id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
  last_name: Kim
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Chen J, Kaufmann W, Chen C, et al. Developmental transformation of Ca2+ channel-vesicle
    nanotopography at a central GABAergic synapse. <i>Neuron</i>. doi:<a href="https://doi.org/10.1016/j.neuron.2023.12.002">10.1016/j.neuron.2023.12.002</a>
  apa: Chen, J., Kaufmann, W., Chen, C., Arai,  itaru, Kim, O., Shigemoto, R., &#38;
    Jonas, P. M. (n.d.). Developmental transformation of Ca2+ channel-vesicle nanotopography
    at a central GABAergic synapse. <i>Neuron</i>. Elsevier. <a href="https://doi.org/10.1016/j.neuron.2023.12.002">https://doi.org/10.1016/j.neuron.2023.12.002</a>
  chicago: Chen, JingJing, Walter Kaufmann, Chong Chen, itaru Arai, Olena Kim, Ryuichi
    Shigemoto, and Peter M Jonas. “Developmental Transformation of Ca2+ Channel-Vesicle
    Nanotopography at a Central GABAergic Synapse.” <i>Neuron</i>. Elsevier, n.d.
    <a href="https://doi.org/10.1016/j.neuron.2023.12.002">https://doi.org/10.1016/j.neuron.2023.12.002</a>.
  ieee: J. Chen <i>et al.</i>, “Developmental transformation of Ca2+ channel-vesicle
    nanotopography at a central GABAergic synapse,” <i>Neuron</i>. Elsevier.
  ista: Chen J, Kaufmann W, Chen C, Arai  itaru, Kim O, Shigemoto R, Jonas PM. Developmental
    transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
    Neuron.
  mla: Chen, JingJing, et al. “Developmental Transformation of Ca2+ Channel-Vesicle
    Nanotopography at a Central GABAergic Synapse.” <i>Neuron</i>, Elsevier, doi:<a
    href="https://doi.org/10.1016/j.neuron.2023.12.002">10.1016/j.neuron.2023.12.002</a>.
  short: J. Chen, W. Kaufmann, C. Chen,  itaru Arai, O. Kim, R. Shigemoto, P.M. Jonas,
    Neuron (n.d.).
date_created: 2024-01-21T23:00:56Z
date_published: 2024-01-11T00:00:00Z
date_updated: 2024-03-05T09:31:24Z
day: '11'
department:
- _id: PeJo
- _id: EM-Fac
- _id: RySh
doi: 10.1016/j.neuron.2023.12.002
ec_funded: 1
external_id:
  pmid:
  - '38215739'
language:
- iso: eng
month: '01'
oa_version: None
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: The Wittgenstein Prize
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
  grant_number: P36232
  name: Mechanisms of GABA release in hippocampal circuits
- _id: 26B66A3E-B435-11E9-9278-68D0E5697425
  grant_number: '25383'
  name: Development of nanodomain coupling between Ca2+ channels and release sensors
    at a central inhibitory synapse
publication: Neuron
publication_identifier:
  eissn:
  - 1097-4199
  issn:
  - 0896-6273
publication_status: inpress
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/synapses-brought-to-the-point/
scopus_import: '1'
status: public
title: Developmental transformation of Ca2+ channel-vesicle nanotopography at a central
  GABAergic synapse
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '11196'
abstract:
- lang: eng
  text: "One of the fundamental questions in Neuroscience is how the structure of
    synapses and their physiological properties are related. While synaptic transmission
    remains a dynamic process, electron microscopy provides images with comparably
    low temporal resolution (Studer et al., 2014). The current work overcomes this
    challenge and describes an improved “Flash and Freeze” technique (Watanabe et
    al., 2013a; Watanabe et al., 2013b) to study synaptic transmission at the hippocampal
    mossy fiber-CA3 pyramidal neuron synapses, using mouse acute brain slices and
    organotypic slices culture. The improved method allowed for selective stimulation
    of presynaptic mossy fiber boutons and the observation of synaptic vesicle pool
    dynamics at the active zones. Our results uncovered several intriguing morphological
    features of mossy fiber boutons. First, the docked vesicle pool was largely depleted
    (more than 70%) after stimulation, implying that the docked synaptic vesicles
    pool and readily releasable pool are vastly overlapping in mossy fiber boutons.
    Second, the synaptic vesicles are skewed towards larger diameters, displaying
    a wide range of sizes. An increase in the mean diameter of synaptic vesicles,
    after single and repetitive stimulation, suggests that smaller vesicles have a
    higher release probability. Third, we observed putative endocytotic structures
    after moderate light stimulation, matching the timing of previously described
    ultrafast endocytosis (Watanabe et al., 2013a; Delvendahl et al., 2016). \r\n\tIn
    addition, synaptic transmission depends on a sophisticated system of protein machinery
    and calcium channels (Südhof, 2013b), which amplifies the challenge in studying
    synaptic communication as these interactions can be potentially modified during
    synaptic plasticity. And although recent study elucidated the potential correlation
    between physiological and morphological properties of synapses during synaptic
    plasticity (Vandael et al., 2020), the molecular underpinning of it remains unknown.
    Thus, the presented work tries to overcome this challenge and aims to pinpoint
    changes in the molecular architecture at hippocampal mossy fiber bouton synapses
    during short- and long-term potentiation (STP and LTP), we combined chemical potentiation,
    with the application of a cyclic adenosine monophosphate agonist (i.e. forskolin)
    and freeze-fracture replica immunolabelling. This method allowed the localization
    of membrane-bound proteins with nanometer precision within the active zone, in
    particular, P/Q-type calcium channels and synaptic vesicle priming proteins Munc13-1/2.
    First, we found that the number of clusters of Munc13-1 in the mossy fiber bouton
    active zone increased significantly during STP, but decreased to lower than the
    control value during LTP. Secondly, although the distance between the calcium
    channels and Munc13-1s did not change after induction of STP, it shortened during
    the LTP phase. Additionally, forskolin did not affect Munc13-2 distribution during
    STP and LTP. These results indicate the existence of two distinct mechanisms that
    govern STP and LTP at mossy fiber bouton synapses: an increase in the readily
    realizable pool in the case of STP and a potential increase in release probability
    during LTP. “Flash and freeze” and functional electron microscopy, are versatile
    methods that can be successfully applied to intact brain circuits to study synaptic
    transmission even at the molecular level.\r\n"
acknowledged_ssus:
- _id: EM-Fac
- _id: PreCl
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Olena
  full_name: Kim, Olena
  id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
  last_name: Kim
citation:
  ama: Kim O. Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron synapses.
    2022. doi:<a href="https://doi.org/10.15479/at:ista:11196">10.15479/at:ista:11196</a>
  apa: Kim, O. (2022). <i>Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal
    neuron synapses</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/at:ista:11196">https://doi.org/10.15479/at:ista:11196</a>
  chicago: Kim, Olena. “Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal
    Neuron Synapses.” Institute of Science and Technology Austria, 2022. <a href="https://doi.org/10.15479/at:ista:11196">https://doi.org/10.15479/at:ista:11196</a>.
  ieee: O. Kim, “Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron
    synapses,” Institute of Science and Technology Austria, 2022.
  ista: Kim O. 2022. Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron
    synapses. Institute of Science and Technology Austria.
  mla: Kim, Olena. <i>Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal Neuron
    Synapses</i>. Institute of Science and Technology Austria, 2022, doi:<a href="https://doi.org/10.15479/at:ista:11196">10.15479/at:ista:11196</a>.
  short: O. Kim, Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal Neuron
    Synapses, Institute of Science and Technology Austria, 2022.
date_created: 2022-04-20T09:47:12Z
date_published: 2022-04-20T00:00:00Z
date_updated: 2023-08-18T06:31:52Z
day: '20'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: PeJo
- _id: GradSch
doi: 10.15479/at:ista:11196
ec_funded: 1
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oa_version: Published Version
page: '132'
project:
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '708497'
  name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
    mossy fiber synapse
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W01205
  name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: The Wittgenstein Prize
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
title: Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron synapses
tmp:
  image: /images/cc_by_nc_nd.png
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type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2022'
...
---
_id: '7473'
abstract:
- lang: eng
  text: How structural and functional properties of synapses relate to each other
    is a fundamental question in neuroscience. Electrophysiology has elucidated mechanisms
    of synaptic transmission, and electron microscopy (EM) has provided insight into
    morphological properties of synapses. Here we describe an enhanced method for
    functional EM (“flash and freeze”), combining optogenetic stimulation with high-pressure
    freezing. We demonstrate that the improved method can be applied to intact networks
    in acute brain slices and organotypic slice cultures from mice. As a proof of
    concept, we probed vesicle pool changes during synaptic transmission at the hippocampal
    mossy fiber-CA3 pyramidal neuron synapse. Our findings show overlap of the docked
    vesicle pool and the functionally defined readily releasable pool and provide
    evidence of fast endocytosis at this synapse. Functional EM with acute slices
    and slice cultures has the potential to reveal the structural and functional mechanisms
    of transmission in intact, genetically perturbed, and disease-affected synapses.
acknowledgement: This project has received funding from the European Research Council
  (ERC) and European Commission (EC), under the European Union’s Horizon 2020 research
  and innovation programme (ERC grant agreement No. 692692 and Marie Sklodowska-Curie
  708497) and from Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27
  Wittgenstein award and DK W1205-B09). We thank Johann Danzl and Ryuichi Shigemoto
  for critically reading the manuscript; Walter Kaufmann, Daniel Gutl, and Vanessa
  Zheden for extensive EM training, advice, and experimental assistance; Benjamin
  Suter for substantial help with light stimulation, ImageJ plugins for analysis,
  and manuscript editing; Florian Marr and Christina Altmutter for technical support;
  Eleftheria Kralli-Beller for manuscript editing; Julia König and Paul Wurzinger
  (Leica Microsystems) for helpful technical discussions; and Taija Makinen for providing
  the Prox1-CreERT2 mouse line.
article_processing_charge: No
article_type: original
author:
- first_name: Carolina
  full_name: Borges Merjane, Carolina
  id: 4305C450-F248-11E8-B48F-1D18A9856A87
  last_name: Borges Merjane
  orcid: 0000-0003-0005-401X
- first_name: Olena
  full_name: Kim, Olena
  id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
  last_name: Kim
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Borges Merjane C, Kim O, Jonas PM. Functional electron microscopy (“Flash and
    Freeze”) of identified cortical synapses in acute brain slices. <i>Neuron</i>.
    2020;105:992-1006. doi:<a href="https://doi.org/10.1016/j.neuron.2019.12.022">10.1016/j.neuron.2019.12.022</a>
  apa: Borges Merjane, C., Kim, O., &#38; Jonas, P. M. (2020). Functional electron
    microscopy (“Flash and Freeze”) of identified cortical synapses in acute brain
    slices. <i>Neuron</i>. Elsevier. <a href="https://doi.org/10.1016/j.neuron.2019.12.022">https://doi.org/10.1016/j.neuron.2019.12.022</a>
  chicago: Borges Merjane, Carolina, Olena Kim, and Peter M Jonas. “Functional Electron
    Microscopy (‘Flash and Freeze’) of Identified Cortical Synapses in Acute Brain
    Slices.” <i>Neuron</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.neuron.2019.12.022">https://doi.org/10.1016/j.neuron.2019.12.022</a>.
  ieee: C. Borges Merjane, O. Kim, and P. M. Jonas, “Functional electron microscopy
    (‘Flash and Freeze’) of identified cortical synapses in acute brain slices,” <i>Neuron</i>,
    vol. 105. Elsevier, pp. 992–1006, 2020.
  ista: Borges Merjane C, Kim O, Jonas PM. 2020. Functional electron microscopy (“Flash
    and Freeze”) of identified cortical synapses in acute brain slices. Neuron. 105,
    992–1006.
  mla: Borges Merjane, Carolina, et al. “Functional Electron Microscopy (‘Flash and
    Freeze’) of Identified Cortical Synapses in Acute Brain Slices.” <i>Neuron</i>,
    vol. 105, Elsevier, 2020, pp. 992–1006, doi:<a href="https://doi.org/10.1016/j.neuron.2019.12.022">10.1016/j.neuron.2019.12.022</a>.
  short: C. Borges Merjane, O. Kim, P.M. Jonas, Neuron 105 (2020) 992–1006.
date_created: 2020-02-10T15:59:45Z
date_published: 2020-03-18T00:00:00Z
date_updated: 2024-03-25T23:30:04Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2019.12.022
ec_funded: 1
external_id:
  isi:
  - '000520854700008'
  pmid:
  - '31928842'
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  checksum: 3582664addf26859e86ac5bec3e01416
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  creator: dernst
  date_created: 2020-11-20T08:58:53Z
  date_updated: 2020-11-20T08:58:53Z
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  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '708497'
  name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
    mossy fiber synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: The Wittgenstein Prize
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W01205
  name: Zellkommunikation in Gesundheit und Krankheit
publication: Neuron
publication_identifier:
  issn:
  - 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/flash-and-freeze-reveals-dynamics-of-nerve-connections/
  record:
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scopus_import: '1'
status: public
title: Functional electron microscopy (“Flash and Freeze”) of identified cortical
  synapses in acute brain slices
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...
---
_id: '11222'
acknowledgement: This work was supported by the ERC and EU Horizon 2020 (ERC 692692;
  MSC-IF 708497) and FWF Z 312-B27 Wittgenstein award; W 1205-B09).
article_number: A3.27
article_processing_charge: No
author:
- first_name: Olena
  full_name: Kim, Olena
  id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
  last_name: Kim
- first_name: Carolina
  full_name: Borges Merjane, Carolina
  id: 4305C450-F248-11E8-B48F-1D18A9856A87
  last_name: Borges Merjane
  orcid: 0000-0003-0005-401X
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: 'Kim O, Borges Merjane C, Jonas PM. Functional analysis of the docked vesicle
    pool in hippocampal mossy fiber terminals by electron microscopy. In: <i>Intrinsic
    Activity</i>. Vol 7. Austrian Pharmacological Society; 2019. doi:<a href="https://doi.org/10.25006/ia.7.s1-a3.27">10.25006/ia.7.s1-a3.27</a>'
  apa: 'Kim, O., Borges Merjane, C., &#38; Jonas, P. M. (2019). Functional analysis
    of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy.
    In <i>Intrinsic Activity</i> (Vol. 7). Innsbruck, Austria: Austrian Pharmacological
    Society. <a href="https://doi.org/10.25006/ia.7.s1-a3.27">https://doi.org/10.25006/ia.7.s1-a3.27</a>'
  chicago: Kim, Olena, Carolina Borges Merjane, and Peter M Jonas. “Functional Analysis
    of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.”
    In <i>Intrinsic Activity</i>, Vol. 7. Austrian Pharmacological Society, 2019.
    <a href="https://doi.org/10.25006/ia.7.s1-a3.27">https://doi.org/10.25006/ia.7.s1-a3.27</a>.
  ieee: O. Kim, C. Borges Merjane, and P. M. Jonas, “Functional analysis of the docked
    vesicle pool in hippocampal mossy fiber terminals by electron microscopy,” in
    <i>Intrinsic Activity</i>, Innsbruck, Austria, 2019, vol. 7, no. Suppl. 1.
  ista: 'Kim O, Borges Merjane C, Jonas PM. 2019. Functional analysis of the docked
    vesicle pool in hippocampal mossy fiber terminals by electron microscopy. Intrinsic
    Activity. ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
    Society vol. 7, A3.27.'
  mla: Kim, Olena, et al. “Functional Analysis of the Docked Vesicle Pool in Hippocampal
    Mossy Fiber Terminals by Electron Microscopy.” <i>Intrinsic Activity</i>, vol.
    7, no. Suppl. 1, A3.27, Austrian Pharmacological Society, 2019, doi:<a href="https://doi.org/10.25006/ia.7.s1-a3.27">10.25006/ia.7.s1-a3.27</a>.
  short: O. Kim, C. Borges Merjane, P.M. Jonas, in:, Intrinsic Activity, Austrian
    Pharmacological Society, 2019.
conference:
  end_date: 2019-09-27
  location: Innsbruck, Austria
  name: 'ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
    Society'
  start_date: 2019-09-25
date_created: 2022-04-20T15:06:05Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2024-03-25T23:30:04Z
day: '11'
department:
- _id: PeJo
doi: 10.25006/ia.7.s1-a3.27
ec_funded: 1
intvolume: '         7'
issue: Suppl. 1
keyword:
- hippocampus
- mossy fibers
- readily releasable pool
- electron microscopy
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.intrinsicactivity.org/2019/7/S1/A3.27/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '708497'
  name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
    mossy fiber synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W01205
  name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: The Wittgenstein Prize
publication: Intrinsic Activity
publication_identifier:
  issn:
  - 2309-8503
publication_status: published
publisher: Austrian Pharmacological Society
quality_controlled: '1'
related_material:
  record:
  - id: '11196'
    relation: dissertation_contains
    status: public
status: public
title: Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals
  by electron microscopy
type: conference_abstract
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 7
year: '2019'
...