---
_id: '7427'
abstract:
- lang: eng
  text: Plants, like other multicellular organisms, survive through a delicate balance
    between growth and defense against pathogens. Salicylic acid (SA) is a major defense
    signal in plants, and the perception mechanism as well as downstream signaling
    activating the immune response are known. Here, we identify a parallel SA signaling
    that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase
    2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin
    transporter is hyperphosphorylated in response to SA, leading to changed activity
    of this important growth regulator. Accordingly, auxin transport and auxin-mediated
    root development, including growth, gravitropic response, and lateral root organogenesis,
    are inhibited. This study reveals how SA, besides activating immunity, concomitantly
    attenuates growth through crosstalk with the auxin distribution network. Further
    analysis of this dual role of SA and characterization of additional SA-regulated
    PP2A targets will provide further insights into mechanisms maintaining a balance
    between growth and defense.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong
  (Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University),
  Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing
  published materials; Jana Riederer for help with cantharidin physiological analysis;
  David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana
  Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´
  for technical support with tobacco cells; Lucia Strader (Washington University),
  Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas
  Mach (BOKU) for helpful discussions; and bioimaging and life science facilities
  of IST Austria for continuous support. We gratefully acknowledge the Nottingham
  Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC
  and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility
  for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research
  leading to these results has received funding from the European Union’s Horizon
  2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie
  Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
  under REA grant agreement no. 291734. S.T. was supported by a European Molecular
  Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N.
  was supported by the Ministry of Education, Youth and Sports of the Czech Republic
  (European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’
  no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional
  Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738).
  J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no.
  CZ.2.16/3.1.00/21519). "
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Melinda F
  full_name: Abas, Melinda F
  id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
  last_name: Abas
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Gergely
  full_name: Molnar, Gergely
  id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
  last_name: Molnar
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Pavel
  full_name: Lasák, Pavel
  last_name: Lasák
- first_name: Ivan
  full_name: Petřík, Ivan
  last_name: Petřík
- first_name: Eugenia
  full_name: Russinova, Eugenia
  last_name: Russinova
- first_name: Jan
  full_name: Petrášek, Jan
  last_name: Petrášek
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Jiří
  full_name: Pospíšil, Jiří
  last_name: Pospíšil
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase
    2A to attenuate growth in plants. <i>Current Biology</i>. 2020;30(3):381-395.e8.
    doi:<a href="https://doi.org/10.1016/j.cub.2019.11.058">10.1016/j.cub.2019.11.058</a>
  apa: Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., …
    Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth
    in plants. <i>Current Biology</i>. Cell Press. <a href="https://doi.org/10.1016/j.cub.2019.11.058">https://doi.org/10.1016/j.cub.2019.11.058</a>
  chicago: Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar,
    Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A
    to Attenuate Growth in Plants.” <i>Current Biology</i>. Cell Press, 2020. <a href="https://doi.org/10.1016/j.cub.2019.11.058">https://doi.org/10.1016/j.cub.2019.11.058</a>.
  ieee: S. Tan <i>et al.</i>, “Salicylic acid targets protein phosphatase 2A to attenuate
    growth in plants,” <i>Current Biology</i>, vol. 30, no. 3. Cell Press, p. 381–395.e8,
    2020.
  ista: Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík
    I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid
    targets protein phosphatase 2A to attenuate growth in plants. Current Biology.
    30(3), 381–395.e8.
  mla: Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate
    Growth in Plants.” <i>Current Biology</i>, vol. 30, no. 3, Cell Press, 2020, p.
    381–395.e8, doi:<a href="https://doi.org/10.1016/j.cub.2019.11.058">10.1016/j.cub.2019.11.058</a>.
  short: S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák,
    I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current
    Biology 30 (2020) 381–395.e8.
date_created: 2020-02-02T23:01:00Z
date_published: 2020-02-03T00:00:00Z
date_updated: 2024-03-25T23:30:20Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2019.11.058
ec_funded: 1
external_id:
  isi:
  - '000511287900018'
  pmid:
  - '31956021'
file:
- access_level: open_access
  checksum: 16f7d51fe28f91c21e4896a2028df40b
  content_type: application/pdf
  creator: dernst
  date_created: 2020-09-22T09:51:28Z
  date_updated: 2020-09-22T09:51:28Z
  file_id: '8555'
  file_name: 2020_CurrentBiology_Tan.pdf
  file_size: 5360135
  relation: main_file
  success: 1
file_date_updated: 2020-09-22T09:51:28Z
has_accepted_license: '1'
intvolume: '        30'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 381-395.e8
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
  grant_number: 723-2015
  name: Long Term Fellowship
publication: Current Biology
publication_identifier:
  issn:
  - '09609822'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
  record:
  - id: '8822'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Salicylic acid targets protein phosphatase 2A to attenuate growth in plants
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 30
year: '2020'
...
---
_id: '191'
abstract:
- lang: eng
  text: Intercellular distribution of the plant hormone auxin largely depends on the
    polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters.
    PIN polarity switches in response to different developmental and environmental
    signals have been shown to redirect auxin fluxes mediating certain developmental
    responses. PIN phosphorylation at different sites and by different kinases is
    crucial for PIN function. Here we investigate the role of PIN phosphorylation
    during gravitropic response. Loss- and gain-of-function mutants in PINOID and
    related kinases but not in D6PK kinase as well as mutations mimicking constitutive
    dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation
    sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic
    bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements
    in response to gravity and during feed-back regulation by auxin itself. Thus PIN
    phosphorylation, besides regulating transport activity and apical-basal targeting,
    is also important for the rapid polarity switches in response to environmental
    and endogenous signals.
article_number: '10279'
article_processing_charge: No
author:
- first_name: Peter
  full_name: Grones, Peter
  id: 399876EC-F248-11E8-B48F-1D18A9856A87
  last_name: Grones
- first_name: Melinda F
  full_name: Abas, Melinda F
  id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
  last_name: Abas
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Angharad
  full_name: Jones, Angharad
  last_name: Jones
- first_name: Sascha
  full_name: Waidmann, Sascha
  last_name: Waidmann
- first_name: Jürgen
  full_name: Kleine Vehn, Jürgen
  last_name: Kleine Vehn
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the
    Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
    <i>Scientific Reports</i>. 2018;8(1). doi:<a href="https://doi.org/10.1038/s41598-018-28188-1">10.1038/s41598-018-28188-1</a>
  apa: Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J.,
    &#38; Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3
    auxin transporter mediates polarity switches during gravitropism. <i>Scientific
    Reports</i>. Springer. <a href="https://doi.org/10.1038/s41598-018-28188-1">https://doi.org/10.1038/s41598-018-28188-1</a>
  chicago: Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann,
    Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
    PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific
    Reports</i>. Springer, 2018. <a href="https://doi.org/10.1038/s41598-018-28188-1">https://doi.org/10.1038/s41598-018-28188-1</a>.
  ieee: P. Grones <i>et al.</i>, “PID/WAG-mediated phosphorylation of the Arabidopsis
    PIN3 auxin transporter mediates polarity switches during gravitropism,” <i>Scientific
    Reports</i>, vol. 8, no. 1. Springer, 2018.
  ista: Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018.
    PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates
    polarity switches during gravitropism. Scientific Reports. 8(1), 10279.
  mla: Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
    PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific
    Reports</i>, vol. 8, no. 1, 10279, Springer, 2018, doi:<a href="https://doi.org/10.1038/s41598-018-28188-1">10.1038/s41598-018-28188-1</a>.
  short: P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J.
    Friml, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:06Z
date_published: 2018-07-06T00:00:00Z
date_updated: 2025-05-07T11:12:31Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41598-018-28188-1
ec_funded: 1
external_id:
  isi:
  - '000437673200053'
file:
- access_level: open_access
  checksum: 266b03f4fb8198e83141617aaa99dcab
  content_type: application/pdf
  creator: dernst
  date_created: 2018-12-17T15:38:56Z
  date_updated: 2020-07-14T12:45:20Z
  file_id: '5714'
  file_name: 2018_ScientificReports_Grones.pdf
  file_size: 2413876
  relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: '         8'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Scientific Reports
publication_status: published
publisher: Springer
publist_id: '7729'
quality_controlled: '1'
related_material:
  record:
  - id: '8822'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter
  mediates polarity switches during gravitropism
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '3012'
abstract:
- lang: eng
  text: Intercellular flow of the phytohormone auxin underpins multiple developmental
    processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein
    (PGP) transporters are crucial factors in auxin transport-related development,
    yet the molecular function of PINs remains unknown. Here, we show that PINs mediate
    auxin efflux from mammalian and yeast cells without needing additional plant-specific
    factors. Conditional gain-of-function alleles and quantitative measurements of
    auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the
    action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to
    auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement
    of PINs in catalyzing cellular auxin efflux.
author:
- first_name: Jan
  full_name: Petrášek, Jan
  last_name: Petrášek
- first_name: Jozef
  full_name: Mravec, Jozef
  last_name: Mravec
- first_name: Rodolphe
  full_name: Bouchard, Rodolphe
  last_name: Bouchard
- first_name: Joshua
  full_name: Blakeslee, Joshua
  last_name: Blakeslee
- first_name: Melinda F
  full_name: Melinda Abas
  id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
  last_name: Abas
- first_name: Daniela
  full_name: Seifertová, Daniela
  last_name: Seifertová
- first_name: Justyna
  full_name: Wiśniewska, Justyna
  last_name: Wiśniewska
- first_name: Zerihun
  full_name: Tadele, Zerihun
  last_name: Tadele
- first_name: Martin
  full_name: Kubeš, Martin
  last_name: Kubeš
- first_name: Milada
  full_name: Čovanová, Milada
  last_name: Čovanová
- first_name: Pankaj
  full_name: Dhonukshe, Pankaj
  last_name: Dhonukshe
- first_name: Petr
  full_name: Skůpa, Petr
  last_name: Skůpa
- first_name: Eva
  full_name: Eva Benková
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Lucie
  full_name: Perry, Lucie
  last_name: Perry
- first_name: Pavel
  full_name: Křeček, Pavel
  last_name: Křeček
- first_name: Ok
  full_name: Lee, Ok Ran
  last_name: Lee
- first_name: Gerald
  full_name: Fink, Gerald R
  last_name: Fink
- first_name: Markus
  full_name: Geisler, Markus
  last_name: Geisler
- first_name: Angus
  full_name: Murphy, Angus S
  last_name: Murphy
- first_name: Christian
  full_name: Luschnig, Christian
  last_name: Luschnig
- first_name: Eva
  full_name: Zažímalová, Eva
  last_name: Zažímalová
- first_name: Jirí
  full_name: Jirí Friml
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting
    function in cellular auxin efflux. <i>Science</i>. 2006;312(5775):914-918. doi:<a
    href="https://doi.org/10.1126/science.1123542">10.1126/science.1123542</a>
  apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová,
    D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular
    auxin efflux. <i>Science</i>. American Association for the Advancement of Science.
    <a href="https://doi.org/10.1126/science.1123542">https://doi.org/10.1126/science.1123542</a>
  chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda
    F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a
    Rate-Limiting Function in Cellular Auxin Efflux.” <i>Science</i>. American Association
    for the Advancement of Science, 2006. <a href="https://doi.org/10.1126/science.1123542">https://doi.org/10.1126/science.1123542</a>.
  ieee: J. Petrášek <i>et al.</i>, “PIN proteins perform a rate-limiting function
    in cellular auxin efflux,” <i>Science</i>, vol. 312, no. 5775. American Association
    for the Advancement of Science, pp. 914–918, 2006.
  ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska
    J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček
    P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006.
    PIN proteins perform a rate-limiting function in cellular auxin efflux. Science.
    312(5775), 914–918.
  mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular
    Auxin Efflux.” <i>Science</i>, vol. 312, no. 5775, American Association for the
    Advancement of Science, 2006, pp. 914–18, doi:<a href="https://doi.org/10.1126/science.1123542">10.1126/science.1123542</a>.
  short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová,
    J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková,
    L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová,
    J. Friml, Science 312 (2006) 914–918.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1123542
extern: 1
intvolume: '       312'
issue: '5775'
month: '05'
page: 914 - 918
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3690'
quality_controlled: 0
status: public
title: PIN proteins perform a rate-limiting function in cellular auxin efflux
type: journal_article
volume: 312
year: '2006'
...