---
_id: '13342'
abstract:
- lang: eng
  text: Motile cells moving in multicellular organisms encounter microenvironments
    of locally heterogeneous mechanochemical composition. Individual compositional
    parameters like chemotactic signals, adhesiveness, and pore sizes are well known
    to be sensed by motile cells, providing individual guidance cues for cellular
    pathfinding. However, motile cells encounter diverse mechanochemical signals at
    the same time, raising the question of how cells respond to locally diverse and
    potentially competing signals on their migration routes. Here, we reveal that
    motile amoeboid cells require nuclear repositioning, termed nucleokinesis, for
    adaptive pathfinding in heterogeneous mechanochemical microenvironments. Using
    mammalian immune cells and the amoeba<jats:italic>Dictyostelium discoideum</jats:italic>,
    we discover that frequent, rapid and long-distance nucleokinesis is a basic component
    of amoeboid pathfinding, enabling cells to reorientate quickly between locally
    competing cues. Amoeboid nucleokinesis comprises a two-step cell polarity switch
    and is driven by myosin II-forces, sliding the nucleus from a ‘losing’ to the
    ‘winning’ leading edge to re-adjust the nuclear to the cellular path. Impaired
    nucleokinesis distorts fast path adaptions and causes cellular arrest in the microenvironment.
    Our findings establish that nucleokinesis is required for amoeboid cell navigation.
    Given that motile single-cell amoebae, many immune cells, and some cancer cells
    utilize an amoeboid migration strategy, these results suggest that amoeboid nucleokinesis
    underlies cellular navigation during unicellular biology, immunity, and disease.
acknowledgement: We thank Christoph Mayr and Bingzhi Wang for initial experiments
  on amoeboid nucleokinesis, Ana-Maria Lennon-Duménil and Aline Yatim for bone marrow
  from MyoIIA-Flox*CD11c-Cre mice, Michael Sixt and Aglaja Kopf for EMTB-mCherry,
  EB3-mCherry, Lifeact-GFP, Lfc knockout, and Myh9-GFP expressing HoxB8 cells, Malte
  Benjamin Braun, Mauricio Ruiz, and Madeleine T. Schmitt for critical reading of
  the manuscript, and the Core Facility Bioimaging, the Core Facility Flow Cytometry,
  and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.
  This study was supported by the Peter Hans Hofschneider Professorship of the foundation
  “Stiftung Experimentelle Biomedizin” (to JR), the LMU Institutional Strategy LMU-Excellent
  within the framework of the German Excellence Initiative (to JR), and the Deutsche
  Forschungsgemeinschaft (DFG; German Research Foundation; SFB914 project A12, to
  JR), and the CZI grant DAF2020-225401 (https://doi.org/10.37921/120055ratwvi) from
  the Chan Zuckerberg Initiative DAF (to RH; an advised fund of Silicon Valley Community
  Foundation (funder https://doi.org/10.13039/100014989)). Open Access funding enabled
  and organized by Projekt DEAL.
article_number: e114557
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Janina
  full_name: Kroll, Janina
  last_name: Kroll
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Arthur
  full_name: Kuznetcov, Arthur
  last_name: Kuznetcov
- first_name: Kasia
  full_name: Stefanowski, Kasia
  last_name: Stefanowski
- first_name: Monika D.
  full_name: Hermann, Monika D.
  last_name: Hermann
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Lubuna B
  full_name: Shafeek, Lubuna B
  id: 3CD37A82-F248-11E8-B48F-1D18A9856A87
  last_name: Shafeek
  orcid: 0000-0001-7180-6050
- first_name: Annette
  full_name: Müller-Taubenberger, Annette
  last_name: Müller-Taubenberger
- first_name: Jörg
  full_name: Renkawitz, Jörg
  id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
  last_name: Renkawitz
  orcid: 0000-0003-2856-3369
citation:
  ama: Kroll J, Hauschild R, Kuznetcov A, et al. Adaptive pathfinding by nucleokinesis
    during amoeboid migration. <i>EMBO Journal</i>. 2023. doi:<a href="https://doi.org/10.15252/embj.2023114557">10.15252/embj.2023114557</a>
  apa: Kroll, J., Hauschild, R., Kuznetcov, A., Stefanowski, K., Hermann, M. D., Merrin,
    J., … Renkawitz, J. (2023). Adaptive pathfinding by nucleokinesis during amoeboid
    migration. <i>EMBO Journal</i>. Embo Press. <a href="https://doi.org/10.15252/embj.2023114557">https://doi.org/10.15252/embj.2023114557</a>
  chicago: Kroll, Janina, Robert Hauschild, Arthur Kuznetcov, Kasia Stefanowski, Monika
    D. Hermann, Jack Merrin, Lubuna B Shafeek, Annette Müller-Taubenberger, and Jörg
    Renkawitz. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.”
    <i>EMBO Journal</i>. Embo Press, 2023. <a href="https://doi.org/10.15252/embj.2023114557">https://doi.org/10.15252/embj.2023114557</a>.
  ieee: J. Kroll <i>et al.</i>, “Adaptive pathfinding by nucleokinesis during amoeboid
    migration,” <i>EMBO Journal</i>. Embo Press, 2023.
  ista: Kroll J, Hauschild R, Kuznetcov A, Stefanowski K, Hermann MD, Merrin J, Shafeek
    LB, Müller-Taubenberger A, Renkawitz J. 2023. Adaptive pathfinding by nucleokinesis
    during amoeboid migration. EMBO Journal., e114557.
  mla: Kroll, Janina, et al. “Adaptive Pathfinding by Nucleokinesis during Amoeboid
    Migration.” <i>EMBO Journal</i>, e114557, Embo Press, 2023, doi:<a href="https://doi.org/10.15252/embj.2023114557">10.15252/embj.2023114557</a>.
  short: J. Kroll, R. Hauschild, A. Kuznetcov, K. Stefanowski, M.D. Hermann, J. Merrin,
    L.B. Shafeek, A. Müller-Taubenberger, J. Renkawitz, EMBO Journal (2023).
date_created: 2023-08-01T08:59:06Z
date_published: 2023-11-21T00:00:00Z
date_updated: 2023-11-27T08:47:45Z
day: '21'
ddc:
- '570'
department:
- _id: NanoFab
- _id: Bio
doi: 10.15252/embj.2023114557
external_id:
  pmid:
  - '37987147'
file:
- access_level: open_access
  checksum: 6261d0041c7e8d284c39712c40079730
  content_type: application/pdf
  creator: dernst
  date_created: 2023-11-27T08:45:56Z
  date_updated: 2023-11-27T08:45:56Z
  file_id: '14611'
  file_name: 2023_EmboJournal_Kroll.pdf
  file_size: 4862497
  relation: main_file
  success: 1
file_date_updated: 2023-11-27T08:45:56Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Journal
publication_identifier:
  eissn:
  - 1460-2075
  issn:
  - 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adaptive pathfinding by nucleokinesis during amoeboid migration
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '12109'
abstract:
- lang: eng
  text: Kelvin probe force microscopy (KPFM) is a powerful tool for studying contact
    electrification (CE) at the nanoscale, but converting KPFM voltage maps to charge
    density maps is nontrivial due to long-range forces and complex system geometry.
    Here we present a strategy using finite-element method (FEM) simulations to determine
    the Green's function of the KPFM probe/insulator/ground system, which allows us
    to quantitatively extract surface charge. Testing our approach with synthetic
    data, we find that accounting for the atomic force microscope (AFM) tip, cone,
    and cantilever is necessary to recover a known input and that existing methods
    lead to gross miscalculation or even the incorrect sign of the underlying charge.
    Applying it to experimental data, we demonstrate its capacity to extract realistic
    surface charge densities and fine details from contact-charged surfaces. Our method
    gives a straightforward recipe to convert qualitative KPFM voltage data into quantitative
    charge data over a range of experimental conditions, enabling quantitative CE
    at the nanoscale.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
acknowledgement: "This project has received funding from the European Research Council
  (ERC) under the European Union’s Horizon 2020 research and innovation programme
  (Grant Agreement\r\nNo. 949120). This research was supported by the Scientific Service
  Units of the Institute of Science and Technology Austria (ISTA) through resources
  provided by the Miba Machine\r\nShop, the Nanofabrication Facility, and the Scientific
  Computing Facility. We thank F. Stumpf from Park Systems for useful discussions
  and support with scanning probe microscopy.\r\nF.P. and J.C.S. contributed equally
  to this work."
article_number: '125605'
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Felix
  full_name: Pertl, Felix
  id: 6313aec0-15b2-11ec-abd3-ed67d16139af
  last_name: Pertl
- first_name: Juan Carlos A
  full_name: Sobarzo Ponce, Juan Carlos A
  id: 4B807D68-AE37-11E9-AC72-31CAE5697425
  last_name: Sobarzo Ponce
- first_name: Lubuna B
  full_name: Shafeek, Lubuna B
  id: 3CD37A82-F248-11E8-B48F-1D18A9856A87
  last_name: Shafeek
  orcid: 0000-0001-7180-6050
- first_name: Tobias
  full_name: Cramer, Tobias
  last_name: Cramer
- first_name: Scott R
  full_name: Waitukaitis, Scott R
  id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
  last_name: Waitukaitis
  orcid: 0000-0002-2299-3176
citation:
  ama: Pertl F, Sobarzo Ponce JCA, Shafeek LB, Cramer T, Waitukaitis SR. Quantifying
    nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid
    approach. <i>Physical Review Materials</i>. 2022;6(12). doi:<a href="https://doi.org/10.1103/PhysRevMaterials.6.125605">10.1103/PhysRevMaterials.6.125605</a>
  apa: Pertl, F., Sobarzo Ponce, J. C. A., Shafeek, L. B., Cramer, T., &#38; Waitukaitis,
    S. R. (2022). Quantifying nanoscale charge density features of contact-charged
    surfaces with an FEM/KPFM-hybrid approach. <i>Physical Review Materials</i>. American
    Physical Society. <a href="https://doi.org/10.1103/PhysRevMaterials.6.125605">https://doi.org/10.1103/PhysRevMaterials.6.125605</a>
  chicago: Pertl, Felix, Juan Carlos A Sobarzo Ponce, Lubuna B Shafeek, Tobias Cramer,
    and Scott R Waitukaitis. “Quantifying Nanoscale Charge Density Features of Contact-Charged
    Surfaces with an FEM/KPFM-Hybrid Approach.” <i>Physical Review Materials</i>.
    American Physical Society, 2022. <a href="https://doi.org/10.1103/PhysRevMaterials.6.125605">https://doi.org/10.1103/PhysRevMaterials.6.125605</a>.
  ieee: F. Pertl, J. C. A. Sobarzo Ponce, L. B. Shafeek, T. Cramer, and S. R. Waitukaitis,
    “Quantifying nanoscale charge density features of contact-charged surfaces with
    an FEM/KPFM-hybrid approach,” <i>Physical Review Materials</i>, vol. 6, no. 12.
    American Physical Society, 2022.
  ista: Pertl F, Sobarzo Ponce JCA, Shafeek LB, Cramer T, Waitukaitis SR. 2022. Quantifying
    nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid
    approach. Physical Review Materials. 6(12), 125605.
  mla: Pertl, Felix, et al. “Quantifying Nanoscale Charge Density Features of Contact-Charged
    Surfaces with an FEM/KPFM-Hybrid Approach.” <i>Physical Review Materials</i>,
    vol. 6, no. 12, 125605, American Physical Society, 2022, doi:<a href="https://doi.org/10.1103/PhysRevMaterials.6.125605">10.1103/PhysRevMaterials.6.125605</a>.
  short: F. Pertl, J.C.A. Sobarzo Ponce, L.B. Shafeek, T. Cramer, S.R. Waitukaitis,
    Physical Review Materials 6 (2022).
date_created: 2023-01-08T23:00:53Z
date_published: 2022-12-29T00:00:00Z
date_updated: 2023-08-03T14:11:29Z
day: '29'
department:
- _id: ScWa
- _id: NanoFab
doi: 10.1103/PhysRevMaterials.6.125605
ec_funded: 1
external_id:
  arxiv:
  - '2209.01889'
  isi:
  - '000908384800001'
intvolume: '         6'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: ' https://doi.org/10.48550/arXiv.2209.01889'
month: '12'
oa: 1
oa_version: Preprint
project:
- _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa
  call_identifier: H2020
  grant_number: '949120'
  name: 'Tribocharge: a multi-scale approach to an enduring problem in physics'
publication: Physical Review Materials
publication_identifier:
  eissn:
  - 2475-9953
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantifying nanoscale charge density features of contact-charged surfaces with
  an FEM/KPFM-hybrid approach
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2022'
...