---
_id: '6178'
abstract:
- lang: eng
  text: Mechanically coupled cells can generate forces driving cell and tissue morphogenesis
    during development. Visualization and measuring of these forces is of major importance
    to better understand the complexity of the biomechanic processes that shape cells
    and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively
    assess mechanical tension in different tissues of the developing zebrafish and
    in cultures of primary germ layer progenitor cells ex vivo.
article_processing_charge: No
author:
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Verena
  full_name: Ruprecht, Verena
  id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
  last_name: Ruprecht
  orcid: 0000-0003-4088-8633
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation
    to measure cell and tissue-generated forces in the zebrafish embryo in vivo and
    ex vivo. In: Nelson C, ed. <i>Tissue Morphogenesis</i>. Vol 1189. Methods in Molecular
    Biology. New York, NY: Springer; 2014:219-235. doi:<a href="https://doi.org/10.1007/978-1-4939-1164-6_15">10.1007/978-1-4939-1164-6_15</a>'
  apa: 'Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., &#38; Heisenberg, C.-P.
    J. (2014). UV laser ablation to measure cell and tissue-generated forces in the
    zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), <i>Tissue Morphogenesis</i>
    (Vol. 1189, pp. 219–235). New York, NY: Springer. <a href="https://doi.org/10.1007/978-1-4939-1164-6_15">https://doi.org/10.1007/978-1-4939-1164-6_15</a>'
  chicago: 'Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and
    Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated
    Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In <i>Tissue Morphogenesis</i>,
    edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York,
    NY: Springer, 2014. <a href="https://doi.org/10.1007/978-1-4939-1164-6_15">https://doi.org/10.1007/978-1-4939-1164-6_15</a>.'
  ieee: 'M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg,
    “UV laser ablation to measure cell and tissue-generated forces in the zebrafish
    embryo in vivo and ex vivo,” in <i>Tissue Morphogenesis</i>, vol. 1189, C. Nelson,
    Ed. New York, NY: Springer, 2014, pp. 219–235.'
  ista: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser
    ablation to measure cell and tissue-generated forces in the zebrafish embryo in
    vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235.'
  mla: Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated
    Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” <i>Tissue Morphogenesis</i>,
    edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:<a href="https://doi.org/10.1007/978-1-4939-1164-6_15">10.1007/978-1-4939-1164-6_15</a>.
  short: M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:,
    C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.
date_created: 2019-03-26T08:55:59Z
date_published: 2014-08-22T00:00:00Z
date_updated: 2023-09-05T14:12:00Z
day: '22'
department:
- _id: CaHe
doi: 10.1007/978-1-4939-1164-6_15
editor:
- first_name: Celeste
  full_name: Nelson, Celeste
  last_name: Nelson
external_id:
  pmid:
  - '25245697'
intvolume: '      1189'
language:
- iso: eng
month: '08'
oa_version: None
page: 219-235
place: New York, NY
pmid: 1
publication: Tissue Morphogenesis
publication_identifier:
  eissn:
  - 1940-6029
  isbn:
  - '9781493911639'
  - '9781493911646'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: Methods in Molecular Biology
status: public
title: UV laser ablation to measure cell and tissue-generated forces in the zebrafish
  embryo in vivo and ex vivo
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 1189
year: '2014'
...
---
_id: '2282'
abstract:
- lang: eng
  text: Epithelial spreading is a common and fundamental aspect of various developmental
    and disease-related processes such as epithelial closure and wound healing. A
    key challenge for epithelial tissues undergoing spreading is to increase their
    surface area without disrupting epithelial integrity. Here we show that orienting
    cell divisions by tension constitutes an efficient mechanism by which the enveloping
    cell layer (EVL) releases anisotropic tension while undergoing spreading during
    zebrafish epiboly. The control of EVL cell-division orientation by tension involves
    cell elongation and requires myosin II activity to align the mitotic spindle with
    the main tension axis. We also found that in the absence of tension-oriented cell
    divisions and in the presence of increased tissue tension, EVL cells undergo ectopic
    fusions, suggesting that the reduction of tension anisotropy by oriented cell
    divisions is required to prevent EVL cells from fusing. We conclude that cell-division
    orientation by tension constitutes a key mechanism for limiting tension anisotropy
    and thus promoting tissue spreading during EVL epiboly.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: 'This work was supported by the IST Austria and MPI-CBG '
author:
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Jonas
  full_name: Ranft, Jonas
  last_name: Ranft
- first_name: Thomas
  full_name: Risler, Thomas
  last_name: Risler
- first_name: Nicolas
  full_name: Minc, Nicolas
  last_name: Minc
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented
    cell divisions limit anisotropic tissue tension in epithelial spreading during
    zebrafish epiboly. <i>Nature Cell Biology</i>. 2013;15:1405-1414. doi:<a href="https://doi.org/10.1038/ncb2869">10.1038/ncb2869</a>
  apa: Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., &#38; Heisenberg,
    C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension
    in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>.
    Nature Publishing Group. <a href="https://doi.org/10.1038/ncb2869">https://doi.org/10.1038/ncb2869</a>
  chicago: Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc,
    and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic
    Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell
    Biology</i>. Nature Publishing Group, 2013. <a href="https://doi.org/10.1038/ncb2869">https://doi.org/10.1038/ncb2869</a>.
  ieee: P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg,
    “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial
    spreading during zebrafish epiboly,” <i>Nature Cell Biology</i>, vol. 15. Nature
    Publishing Group, pp. 1405–1414, 2013.
  ista: Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented
    cell divisions limit anisotropic tissue tension in epithelial spreading during
    zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.
  mla: Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic
    Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell
    Biology</i>, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:<a href="https://doi.org/10.1038/ncb2869">10.1038/ncb2869</a>.
  short: P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg,
    Nature Cell Biology 15 (2013) 1405–1414.
date_created: 2018-12-11T11:56:45Z
date_published: 2013-11-10T00:00:00Z
date_updated: 2023-02-21T17:02:44Z
day: '10'
department:
- _id: CaHe
doi: 10.1038/ncb2869
intvolume: '        15'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://hal.upmc.fr/hal-00983313/
month: '11'
oa: 1
oa_version: Submitted Version
page: 1405 - 1414
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '4652'
quality_controlled: '1'
related_material:
  record:
  - id: '1403'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial
  spreading during zebrafish epiboly
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2013'
...
---
_id: '2286'
abstract:
- lang: eng
  text: The spatiotemporal control of cell divisions is a key factor in epithelial
    morphogenesis and patterning. Mao et al (2013) now describe how differential rates
    of proliferation within the Drosophila wing disc epithelium give rise to anisotropic
    tissue tension in peripheral/proximal regions of the disc. Such global tissue
    tension anisotropy in turn determines the orientation of cell divisions by controlling
    epithelial cell elongation.
author:
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Campinho P, Heisenberg C-PJ. The force and effect of cell proliferation. <i>EMBO
    Journal</i>. 2013;32(21):2783-2784. doi:<a href="https://doi.org/10.1038/emboj.2013.225">10.1038/emboj.2013.225</a>
  apa: Campinho, P., &#38; Heisenberg, C.-P. J. (2013). The force and effect of cell
    proliferation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href="https://doi.org/10.1038/emboj.2013.225">https://doi.org/10.1038/emboj.2013.225</a>
  chicago: Campinho, Pedro, and Carl-Philipp J Heisenberg. “The Force and Effect of
    Cell Proliferation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href="https://doi.org/10.1038/emboj.2013.225">https://doi.org/10.1038/emboj.2013.225</a>.
  ieee: P. Campinho and C.-P. J. Heisenberg, “The force and effect of cell proliferation,”
    <i>EMBO Journal</i>, vol. 32, no. 21. Wiley-Blackwell, pp. 2783–2784, 2013.
  ista: Campinho P, Heisenberg C-PJ. 2013. The force and effect of cell proliferation.
    EMBO Journal. 32(21), 2783–2784.
  mla: Campinho, Pedro, and Carl-Philipp J. Heisenberg. “The Force and Effect of Cell
    Proliferation.” <i>EMBO Journal</i>, vol. 32, no. 21, Wiley-Blackwell, 2013, pp.
    2783–84, doi:<a href="https://doi.org/10.1038/emboj.2013.225">10.1038/emboj.2013.225</a>.
  short: P. Campinho, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 2783–2784.
date_created: 2018-12-11T11:56:46Z
date_published: 2013-10-04T00:00:00Z
date_updated: 2021-01-12T06:56:32Z
day: '04'
department:
- _id: CaHe
doi: 10.1038/emboj.2013.225
external_id:
  pmid:
  - '24097062'
intvolume: '        32'
issue: '21'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817470/
month: '10'
oa: 1
oa_version: Submitted Version
page: 2783 - 2784
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4645'
quality_controlled: '1'
scopus_import: 1
status: public
title: The force and effect of cell proliferation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2013'
...
---
_id: '1406'
abstract:
- lang: eng
  text: Epithelial spreading is a critical part of various developmental and wound
    repair processes. Here we use zebrafish epiboly as a model system to study the
    cellular and molecular mechanisms underlying the spreading of epithelial sheets.
    During zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium,
    spreads over the embryo to eventually cover the entire yolk cell by the end of
    gastrulation. The EVL leading edge is anchored through tight junctions to the
    yolk syncytial layer (YSL), where directly adjacent to the EVL margin a contractile
    actomyosin ring is formed that is thought to drive EVL epiboly. The prevalent
    view in the field was that the contractile ring exerts a pulling force on the
    EVL margin, which pulls the EVL towards the vegetal pole. However, how this force
    is generated and how it affects EVL morphology still remains elusive. Moreover,
    the cellular mechanisms mediating the increase in EVL surface area, while maintaining
    tissue integrity and function are still unclear. Here we show that the YSL actomyosin
    ring pulls on the EVL margin by two distinct force-generating mechanisms. One
    mechanism is based on contraction of the ring around its circumference, as previously
    proposed. The second mechanism is based on actomyosin retrogade flows, generating
    force through resistance against the substrate. The latter can function at any
    epiboly stage even in situations where the contraction-based mechanism is unproductive.
    Additionally, we demonstrate that during epiboly the EVL is subjected to anisotropic
    tension, which guides the orientation of EVL cell division along the main axis
    (animal-vegetal) of tension. The influence of tension in cell division orientation
    involves cell elongation and requires myosin-2 activity for proper spindle alignment.
    Strikingly, we reveal that tension-oriented cell divisions release anisotropic
    tension within the EVL and that in the absence of such divisions, EVL cells undergo
    ectopic fusions. We conclude that forces applied to the EVL by the action of the
    YSL actomyosin ring generate a tension anisotropy in the EVL that orients cell
    divisions, which in turn limit tissue tension increase thereby facilitating tissue
    spreading.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
citation:
  ama: 'Campinho P. Mechanics of zebrafish epiboly: Tension-oriented cell divisions
    limit anisotropic tissue tension in epithelial spreading. 2013.'
  apa: 'Campinho, P. (2013). <i>Mechanics of zebrafish epiboly: Tension-oriented cell
    divisions limit anisotropic tissue tension in epithelial spreading</i>. Institute
    of Science and Technology Austria.'
  chicago: 'Campinho, Pedro. “Mechanics of Zebrafish Epiboly: Tension-Oriented Cell
    Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading.” Institute
    of Science and Technology Austria, 2013.'
  ieee: 'P. Campinho, “Mechanics of zebrafish epiboly: Tension-oriented cell divisions
    limit anisotropic tissue tension in epithelial spreading,” Institute of Science
    and Technology Austria, 2013.'
  ista: 'Campinho P. 2013. Mechanics of zebrafish epiboly: Tension-oriented cell divisions
    limit anisotropic tissue tension in epithelial spreading. Institute of Science
    and Technology Austria.'
  mla: 'Campinho, Pedro. <i>Mechanics of Zebrafish Epiboly: Tension-Oriented Cell
    Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading</i>. Institute
    of Science and Technology Austria, 2013.'
  short: 'P. Campinho, Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions
    Limit Anisotropic Tissue Tension in Epithelial Spreading, Institute of Science
    and Technology Austria, 2013.'
date_created: 2018-12-11T11:51:50Z
date_published: 2013-10-01T00:00:00Z
date_updated: 2023-09-07T11:36:07Z
day: '01'
degree_awarded: PhD
department:
- _id: CaHe
language:
- iso: eng
month: '10'
oa_version: None
page: '123'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '5801'
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: 'Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic
  tissue tension in epithelial spreading'
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2013'
...
---
_id: '2950'
abstract:
- lang: eng
  text: Contractile actomyosin rings drive various fundamental morphogenetic processes
    ranging from cytokinesis to wound healing. Actomyosin rings are generally thought
    to function by circumferential contraction. Here, we show that the spreading of
    the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation
    is driven by a contractile actomyosin ring. In contrast to previous suggestions,
    we find that this ring functions not only by circumferential contraction but also
    by a flow-friction mechanism. This generates a pulling force through resistance
    against retrograde actomyosin flow. EVL spreading proceeds normally in situations
    where circumferential contraction is unproductive, indicating that the flow-friction
    mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis
    through a combination of cable-constriction and flow-friction mechanisms.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Felix
  full_name: Oswald, Felix
  last_name: Oswald
- first_name: Julia
  full_name: Roensch, Julia
  id: 4220E59C-F248-11E8-B48F-1D18A9856A87
  last_name: Roensch
- first_name: Stephan
  full_name: Grill, Stephan
  last_name: Grill
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading
    in zebrafish gastrulation. <i>Science</i>. 2012;338(6104):257-260. doi:<a href="https://doi.org/10.1126/science.1224143">10.1126/science.1224143</a>
  apa: Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch,
    J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish
    gastrulation. <i>Science</i>. American Association for the Advancement of Science.
    <a href="https://doi.org/10.1126/science.1224143">https://doi.org/10.1126/science.1224143</a>
  chicago: Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild,
    Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces
    Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>. American
    Association for the Advancement of Science, 2012. <a href="https://doi.org/10.1126/science.1224143">https://doi.org/10.1126/science.1224143</a>.
  ieee: M. Behrndt <i>et al.</i>, “Forces driving epithelial spreading in zebrafish
    gastrulation,” <i>Science</i>, vol. 338, no. 6104. American Association for the
    Advancement of Science, pp. 257–260, 2012.
  ista: Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill
    S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation.
    Science. 338(6104), 257–260.
  mla: Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.”
    <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of
    Science, 2012, pp. 257–60, doi:<a href="https://doi.org/10.1126/science.1224143">10.1126/science.1224143</a>.
  short: M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch,
    S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.
date_created: 2018-12-11T12:00:30Z
date_published: 2012-10-12T00:00:00Z
date_updated: 2023-02-21T17:02:44Z
day: '12'
department:
- _id: CaHe
- _id: Bio
doi: 10.1126/science.1224143
intvolume: '       338'
issue: '6104'
language:
- iso: eng
month: '10'
oa_version: None
page: 257 - 260
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3778'
quality_controlled: '1'
related_material:
  record:
  - id: '1403'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Forces driving epithelial spreading in zebrafish gastrulation
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 338
year: '2012'
...