[{"date_created":"2018-12-11T11:54:40Z","department":[{"_id":"MiSi"}],"publisher":"Wiley-Blackwell","title":"Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2","scopus_import":1,"language":[{"iso":"eng"}],"month":"02","doi":"10.1002/eji.201343681","year":"2014","date_published":"2014-02-01T00:00:00Z","_id":"1910","acknowledgement":"FWF. Grant Number: P22058-B20","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","oa_version":"None","issue":"2","publication":"European Journal of Immunology","date_updated":"2021-01-12T06:54:01Z","volume":44,"publist_id":"5185","page":"553 - 560","intvolume":"        44","abstract":[{"text":"angerhans cells (LCs) are a unique subset of dendritic cells (DCs) that express epithelial adhesion molecules, allowing them to form contacts with epithelial cells and reside in epidermal/epithelial tissues. The dynamic regulation of epithelial adhesion plays a decisive role in the life cycle of LCs. It controls whether LCs remain immature and sessile within the epidermis or mature and egress to initiate immune responses. So far, the molecular machinery regulating epithelial adhesion molecules during LC maturation remains elusive. Here, we generated pure populations of immature human LCs in vitro to systematically probe for gene-expression changes during LC maturation. LCs down-regulate a set of epithelial genes including E-cadherin, while they upregulate the mesenchymal marker N-cadherin known to facilitate cell migration. In addition, N-cadherin is constitutively expressed by monocyte-derived DCs known to exhibit characteristics of both inflammatory-type and interstitial/dermal DCs. Moreover, the transcription factors ZEB1 and ZEB2 (ZEB is zinc-finger E-box-binding homeobox) are upregulated in migratory LCs. ZEB1 and ZEB2 have been shown to induce epithelial-to-mesenchymal transition (EMT) and invasive behavior in cancer cells undergoing metastasis. Our results provide the first hint that the molecular EMT machinery might facilitate LC mobilization. Moreover, our study suggests that N-cadherin plays a role during DC migration.","lang":"eng"}],"author":[{"full_name":"Konradi, Sabine","last_name":"Konradi","first_name":"Sabine"},{"full_name":"Yasmin, Nighat","last_name":"Yasmin","first_name":"Nighat"},{"full_name":"Haslwanter, Denise","last_name":"Haslwanter","first_name":"Denise"},{"last_name":"Weber","full_name":"Weber, Michele","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gesslbauer","full_name":"Gesslbauer, Bernd","first_name":"Bernd"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Strobl","full_name":"Strobl, Herbert","first_name":"Herbert"}],"status":"public","publication_status":"published","day":"01","citation":{"ista":"Konradi S, Yasmin N, Haslwanter D, Weber M, Gesslbauer B, Sixt MK, Strobl H. 2014. Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. European Journal of Immunology. 44(2), 553–560.","short":"S. Konradi, N. Yasmin, D. Haslwanter, M. Weber, B. Gesslbauer, M.K. Sixt, H. Strobl, European Journal of Immunology 44 (2014) 553–560.","ama":"Konradi S, Yasmin N, Haslwanter D, et al. Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. <i>European Journal of Immunology</i>. 2014;44(2):553-560. doi:<a href=\"https://doi.org/10.1002/eji.201343681\">10.1002/eji.201343681</a>","mla":"Konradi, Sabine, et al. “Langerhans Cell Maturation Is Accompanied by Induction of N-Cadherin and the Transcriptional Regulators of Epithelial-Mesenchymal Transition ZEB1/2.” <i>European Journal of Immunology</i>, vol. 44, no. 2, Wiley-Blackwell, 2014, pp. 553–60, doi:<a href=\"https://doi.org/10.1002/eji.201343681\">10.1002/eji.201343681</a>.","chicago":"Konradi, Sabine, Nighat Yasmin, Denise Haslwanter, Michele Weber, Bernd Gesslbauer, Michael K Sixt, and Herbert Strobl. “Langerhans Cell Maturation Is Accompanied by Induction of N-Cadherin and the Transcriptional Regulators of Epithelial-Mesenchymal Transition ZEB1/2.” <i>European Journal of Immunology</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/eji.201343681\">https://doi.org/10.1002/eji.201343681</a>.","ieee":"S. Konradi <i>et al.</i>, “Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2,” <i>European Journal of Immunology</i>, vol. 44, no. 2. Wiley-Blackwell, pp. 553–560, 2014.","apa":"Konradi, S., Yasmin, N., Haslwanter, D., Weber, M., Gesslbauer, B., Sixt, M. K., &#38; Strobl, H. (2014). Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. <i>European Journal of Immunology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/eji.201343681\">https://doi.org/10.1002/eji.201343681</a>"},"type":"journal_article"},{"year":"2013","doi":"10.1126/science.1228456","ec_funded":1,"title":"Interstitial dendritic cell guidance by haptotactic chemokine gradients","main_file_link":[{"open_access":"1","url":"https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf"}],"citation":{"ista":"Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. 339(6117), 328–332.","short":"M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler, S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.","ama":"Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. 2013;339(6117):328-332. doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>","mla":"Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>, vol. 339, no. 6117, American Association for the Advancement of Science, 2013, pp. 328–32, doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>.","chicago":"Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>. American Association for the Advancement of Science, 2013. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>.","ieee":"M. Weber <i>et al.</i>, “Interstitial dendritic cell guidance by haptotactic chemokine gradients,” <i>Science</i>, vol. 339, no. 6117. American Association for the Advancement of Science, pp. 328–332, 2013.","apa":"Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler, D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>"},"publication_status":"published","abstract":[{"text":"Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues.","lang":"eng"}],"author":[{"id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","first_name":"Jan","full_name":"Schwarz, Jan","last_name":"Schwarz"},{"first_name":"Christine","full_name":"Moussion, Christine","last_name":"Moussion","id":"3356F664-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries","full_name":"De Vries, Ingrid","first_name":"Ingrid"},{"last_name":"Legler","full_name":"Legler, Daniel","first_name":"Daniel"},{"first_name":"Sanjiv","full_name":"Luther, Sanjiv","last_name":"Luther"},{"last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X","first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"article_processing_charge":"No","oa":1,"volume":339,"date_updated":"2022-06-10T10:21:40Z","publist_id":"3959","_id":"2839","oa_version":"Published Version","quality_controlled":"1","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7","grant_number":"281556"},{"name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425","grant_number":"RGP0058/2011"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst, and E. Kiermaier for critical reading of the manuscript. This work was supported by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011 to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).","month":"01","date_published":"2013-01-18T00:00:00Z","article_type":"original","scopus_import":"1","publisher":"American Association for the Advancement of Science","language":[{"iso":"eng"}],"department":[{"_id":"MiSi"},{"_id":"Bio"}],"date_created":"2018-12-11T11:59:52Z","day":"18","type":"journal_article","intvolume":"       339","status":"public","publication":"Science","issue":"6117","page":"328 - 332"},{"pmid":1,"_id":"10900","publication_identifier":{"isbn":["9781627034258"],"issn":["1064-3745"],"eisbn":["9781627034265"],"eissn":["1940-6029"]},"acknowledgement":"We would like to thank Alexander Eichner and Ingrid de Vries for discussion and critical reading of the manuscript, and Mary Frank for assistance with the recording of videos and images in Fig. 1. M.S. is supported through funding from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt Foundation for funding.","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"None","quality_controlled":"1","editor":[{"first_name":"Astrid","full_name":"Cardona, Astrid","last_name":"Cardona"},{"full_name":"Ubogu, Eroboghene","last_name":"Ubogu","first_name":"Eroboghene"}],"date_updated":"2023-09-05T13:15:33Z","volume":1013,"article_processing_charge":"No","abstract":[{"text":"Leukocyte migration through the interstitial space is crucial for the maintenance of tolerance and immunity. The main cues for leukocyte trafficking are chemokines thought to directionally guide these cells towards their targets. However, model systems that facilitate quantification of chemokine-guided leukocyte migration in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted mouse ears that allows the visualization of chemokine-dependent dendritic cell (DC) motility in the dermal interstitium in real time. We present methods for the preparation of mouse ear sheets and their use in multidimensional confocal imaging experiments to monitor and analyze the directional migration of fluorescently labelled DCs through the dermis and into afferent lymphatic vessels. The assay provides a more physiological approach to study leukocyte migration than in vitro three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen gels and transwell assays.","lang":"eng"}],"author":[{"last_name":"Weber","full_name":"Weber, Michele","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K"}],"publication_status":"published","citation":{"mla":"Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26, doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>.","ama":"Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. <i>Chemokines</i>. Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>","ista":"Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology, vol. 1013, 215–226.","short":"M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana Press, Totowa, NJ, 2013, pp. 215–226.","ieee":"M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations,” in <i>Chemokines</i>, vol. 1013, A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.","apa":"Weber, M., &#38; Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona &#38; E. Ubogu (Eds.), <i>Chemokines</i> (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>","chicago":"Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ: Humana Press, 2013. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>."},"alternative_title":["Methods in Molecular Biology"],"title":"Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations","external_id":{"pmid":["23625502"]},"year":"2013","place":"Totowa, NJ","doi":"10.1007/978-1-62703-426-5_14","publication":"Chemokines","page":"215-226","intvolume":"      1013","status":"public","day":"03","type":"book_chapter","series_title":"MIMB","date_created":"2022-03-21T07:47:41Z","department":[{"_id":"MiSi"}],"publisher":"Humana Press","scopus_import":"1","language":[{"iso":"eng"}],"month":"04","date_published":"2013-04-03T00:00:00Z"},{"department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:46:57Z","doi":"10.1016/j.imlet.2013.07.007","month":"07","year":"2013","date_published":"2013-07-01T00:00:00Z","publisher":"Elsevier","scopus_import":1,"title":"Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells","language":[{"iso":"eng"}],"issue":"1-2","publication":"Immunology Letters","volume":154,"publist_id":"7300","date_updated":"2021-01-12T08:01:22Z","page":"31 - 41","_id":"522","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","oa_version":"None","publication_status":"published","citation":{"chicago":"Fuertbauer, Elke, Jan Zaujec, Pavel Uhrin, Ingrid Raab, Michele Weber, Helga Schachner, Miroslav Bauer, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>.","ieee":"E. Fuertbauer <i>et al.</i>, “Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells,” <i>Immunology Letters</i>, vol. 154, no. 1–2. Elsevier, pp. 31–41, 2013.","apa":"Fuertbauer, E., Zaujec, J., Uhrin, P., Raab, I., Weber, M., Schachner, H., … Stockinger, H. (2013). Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>","ista":"Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, Bauer M, Schütz G, Binder B, Sixt MK, Kerjaschki D, Stockinger H. 2013. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. Immunology Letters. 154(1–2), 31–41.","short":"E. Fuertbauer, J. Zaujec, P. Uhrin, I. Raab, M. Weber, H. Schachner, M. Bauer, G. Schütz, B. Binder, M.K. Sixt, D. Kerjaschki, H. Stockinger, Immunology Letters 154 (2013) 31–41.","ama":"Fuertbauer E, Zaujec J, Uhrin P, et al. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. 2013;154(1-2):31-41. doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>","mla":"Fuertbauer, Elke, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>, vol. 154, no. 1–2, Elsevier, 2013, pp. 31–41, doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>."},"day":"01","type":"journal_article","intvolume":"       154","abstract":[{"lang":"eng","text":"Podoplanin, a mucin-like plasma membrane protein, is expressed by lymphatic endothelial cells and responsible for separation of blood and lymphatic circulation through activation of platelets. Here we show that podoplanin is also expressed by thymic fibroblastic reticular cells (tFRC), a novel thymic medulla stroma cell type associated with thymic conduits, and involved in development of natural regulatory T cells (nTreg). Young mice deficient in podoplanin lack nTreg owing to retardation of CD4+CD25+ thymocytes in the cortex and missing differentiation of Foxp3+ thymocytes in the medulla. This might be due to CCL21 that delocalizes upon deletion of the CCL21-binding podoplanin from medullar tFRC to cortex areas. The animals do not remain devoid of nTreg but generate them delayed within the first month resulting in Th2-biased hypergammaglobulinemia but not in the death-causing autoimmune phenotype of Foxp3-deficient Scurfy mice."}],"status":"public","author":[{"full_name":"Fuertbauer, Elke","last_name":"Fuertbauer","first_name":"Elke"},{"last_name":"Zaujec","full_name":"Zaujec, Jan","first_name":"Jan"},{"last_name":"Uhrin","full_name":"Uhrin, Pavel","first_name":"Pavel"},{"first_name":"Ingrid","last_name":"Raab","full_name":"Raab, Ingrid"},{"id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele"},{"last_name":"Schachner","full_name":"Schachner, Helga","first_name":"Helga"},{"first_name":"Miroslav","full_name":"Bauer, Miroslav","last_name":"Bauer"},{"first_name":"Gerhard","full_name":"Schütz, Gerhard","last_name":"Schütz"},{"last_name":"Binder","full_name":"Binder, Bernd","first_name":"Bernd"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K"},{"first_name":"Dontscho","full_name":"Kerjaschki, Dontscho","last_name":"Kerjaschki"},{"first_name":"Hannes","full_name":"Stockinger, Hannes","last_name":"Stockinger"}]},{"date_created":"2018-12-11T12:01:47Z","department":[{"_id":"MiSi"}],"language":[{"iso":"eng"}],"external_id":{"pmid":["22491839"]},"title":"NextGen speaks 13 ","publisher":"American Association for the Advancement of Science","article_type":"letter_note","date_published":"2012-04-06T00:00:00Z","year":"2012","month":"04","doi":"10.1126/science.336.6077.32","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"_id":"3167","page":"32-34","popular_science":"1","volume":336,"publist_id":"3516","date_updated":"2021-01-12T07:41:32Z","publication":"Science","issue":"6077","author":[{"full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"}],"status":"public","intvolume":"       336","type":"journal_article","day":"06","citation":{"chicago":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>.","apa":"Weber, M. (2012). NextGen speaks 13 . <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>","ieee":"M. Weber, “NextGen speaks 13 ,” <i>Science</i>, vol. 336, no. 6077. American Association for the Advancement of Science, pp. 32–34, 2012.","ista":"Weber M. 2012. NextGen speaks 13 . Science. 336(6077), 32–34.","short":"M. Weber, Science 336 (2012) 32–34.","mla":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>, vol. 336, no. 6077, American Association for the Advancement of Science, 2012, pp. 32–34, doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>.","ama":"Weber M. NextGen speaks 13 . <i>Science</i>. 2012;336(6077):32-34. doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>"},"publication_status":"published"},{"quality_controlled":0,"_id":"3960","extern":1,"date_updated":"2021-01-12T07:53:29Z","volume":29,"publist_id":"2167","oa":1,"page":"2861 - 2863","issue":"17","publication":"EMBO Journal","status":"public","author":[{"last_name":"Weber","full_name":"Michele Weber","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Michael Sixt","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"intvolume":"        29","abstract":[{"text":"When lymphocytes follow chemotactic cues, they can adopt different migratory modes depending on the geometry and molecular composition of their extracellular environment. In this issue of The EMBO Journal, Klemke et al (2010) describe a novel Ras-dependent chemokine receptor signalling pathway that leads to activation of cofilin, which in turn amplifies actin turnover. This signalling module is exclusively required for lymphocyte migration in three-dimensional (3D) environments, but not for locomotion on two-dimensional (2D) surfaces.","lang":"eng"}],"type":"journal_article","publication_status":"published","day":"01","citation":{"short":"M. Weber, M.K. Sixt, EMBO Journal 29 (2010) 2861–2863.","ista":"Weber M, Sixt MK. 2010. MEK signalling tunes actin treadmilling for interstitial lymphocyte migration. EMBO Journal. 29(17), 2861–2863.","mla":"Weber, Michele, and Michael K. Sixt. “MEK Signalling Tunes Actin Treadmilling for Interstitial Lymphocyte Migration.” <i>EMBO Journal</i>, vol. 29, no. 17, Wiley-Blackwell, 2010, pp. 2861–63, doi:<a href=\"https://doi.org/10.1038/emboj.2010.183\">10.1038/emboj.2010.183</a>.","ama":"Weber M, Sixt MK. MEK signalling tunes actin treadmilling for interstitial lymphocyte migration. <i>EMBO Journal</i>. 2010;29(17):2861-2863. doi:<a href=\"https://doi.org/10.1038/emboj.2010.183\">10.1038/emboj.2010.183</a>","chicago":"Weber, Michele, and Michael K Sixt. “MEK Signalling Tunes Actin Treadmilling for Interstitial Lymphocyte Migration.” <i>EMBO Journal</i>. Wiley-Blackwell, 2010. <a href=\"https://doi.org/10.1038/emboj.2010.183\">https://doi.org/10.1038/emboj.2010.183</a>.","apa":"Weber, M., &#38; Sixt, M. K. (2010). MEK signalling tunes actin treadmilling for interstitial lymphocyte migration. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2010.183\">https://doi.org/10.1038/emboj.2010.183</a>","ieee":"M. Weber and M. K. Sixt, “MEK signalling tunes actin treadmilling for interstitial lymphocyte migration,” <i>EMBO Journal</i>, vol. 29, no. 17. Wiley-Blackwell, pp. 2861–2863, 2010."},"date_created":"2018-12-11T12:06:07Z","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/issues/190105/"}],"publisher":"Wiley-Blackwell","title":"MEK signalling tunes actin treadmilling for interstitial lymphocyte migration","date_published":"2010-09-01T00:00:00Z","year":"2010","month":"09","doi":"10.1038/emboj.2010.183"},{"acknowledgement":"We thank S. Cremer for statistical analysis, K. Hirsch for technical assistance, D. Critchley for talin1-deficient mice and R. Fässler for integrindeficient mice, discussions and critical reading of the manuscript. This work was supported by the German Research Foundation, the Peter Hans Hofschneider Foundation for Experimental Biomedicine, the Max Planck Society, the Alexander von Humboldt Foundation and the allergology programme of the Landesstiftung Baden-Württemberg.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"None","_id":"3954","extern":"1","volume":11,"publist_id":"2173","date_updated":"2021-01-12T07:53:27Z","page":"1438 - 1443","issue":"12","publication":"Nature Cell Biology","status":"public","author":[{"orcid":"0000-0003-2856-3369","last_name":"Renkawitz","full_name":"Renkawitz, Jörg","first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87"},{"id":"F44D762E-4F9D-11E9-B64C-9EB26CEFFB5F","full_name":"Schumann, Kathrin","last_name":"Schumann","first_name":"Kathrin"},{"id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","last_name":"Weber","full_name":"Weber, Michele","first_name":"Michele"},{"full_name":"Lämmermann, Tim","last_name":"Lämmermann","first_name":"Tim"},{"full_name":"Pflicke, Holger","last_name":"Pflicke","first_name":"Holger"},{"first_name":"Matthieu","last_name":"Piel","full_name":"Piel, Matthieu"},{"first_name":"Julien","last_name":"Polleux","full_name":"Polleux, Julien"},{"full_name":"Spatz, Joachim","last_name":"Spatz","first_name":"Joachim"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K"}],"abstract":[{"lang":"eng","text":"The leading front of a cell can either protrude as an actin-free membrane bleb that is inflated by actomyosin-driven contractile forces, or as an actin-rich pseudopodium, a site where polymerizing actin filaments push out the membrane. Pushing filaments can only cause the membrane to protrude if the expanding actin network experiences a retrograde counter-force, which is usually provided by transmembrane receptors of the integrin family. Here we show that chemotactic dendritic cells mechanically adapt to the adhesive properties of their substrate by switching between integrin-mediated and integrin-independent locomotion. We found that on engaging the integrin-actin clutch, actin polymerization was entirely turned into protrusion, whereas on disengagement actin underwent slippage and retrograde flow. Remarkably, accelerated retrograde flow was balanced by an increased actin polymerization rate; therefore, cell shape and protrusion velocity remained constant on alternating substrates. Due to this adaptive response in polymerization dynamics, tracks of adhesive substrate did not dictate the path of the cells. Instead, directional guidance was exclusively provided by a soluble gradient of chemoattractant, which endowed these 'amoeboid' cells with extraordinary flexibility, enabling them to traverse almost every type of tissue."}],"intvolume":"        11","type":"journal_article","publication_status":"published","citation":{"ieee":"J. Renkawitz <i>et al.</i>, “Adaptive force transmission in amoeboid cell migration,” <i>Nature Cell Biology</i>, vol. 11, no. 12. Nature Publishing Group, pp. 1438–1443, 2009.","apa":"Renkawitz, J., Schumann, K., Weber, M., Lämmermann, T., Pflicke, H., Piel, M., … Sixt, M. K. (2009). Adaptive force transmission in amoeboid cell migration. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb1992\">https://doi.org/10.1038/ncb1992</a>","chicago":"Renkawitz, Jörg, Kathrin Schumann, Michele Weber, Tim Lämmermann, Holger Pflicke, Matthieu Piel, Julien Polleux, Joachim Spatz, and Michael K Sixt. “Adaptive Force Transmission in Amoeboid Cell Migration.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2009. <a href=\"https://doi.org/10.1038/ncb1992\">https://doi.org/10.1038/ncb1992</a>.","ama":"Renkawitz J, Schumann K, Weber M, et al. Adaptive force transmission in amoeboid cell migration. <i>Nature Cell Biology</i>. 2009;11(12):1438-1443. doi:<a href=\"https://doi.org/10.1038/ncb1992\">10.1038/ncb1992</a>","mla":"Renkawitz, Jörg, et al. “Adaptive Force Transmission in Amoeboid Cell Migration.” <i>Nature Cell Biology</i>, vol. 11, no. 12, Nature Publishing Group, 2009, pp. 1438–43, doi:<a href=\"https://doi.org/10.1038/ncb1992\">10.1038/ncb1992</a>.","short":"J. Renkawitz, K. Schumann, M. Weber, T. Lämmermann, H. Pflicke, M. Piel, J. Polleux, J. Spatz, M.K. Sixt, Nature Cell Biology 11 (2009) 1438–1443.","ista":"Renkawitz J, Schumann K, Weber M, Lämmermann T, Pflicke H, Piel M, Polleux J, Spatz J, Sixt MK. 2009. Adaptive force transmission in amoeboid cell migration. Nature Cell Biology. 11(12), 1438–1443."},"day":"15","date_created":"2018-12-11T12:06:05Z","language":[{"iso":"eng"}],"publisher":"Nature Publishing Group","title":"Adaptive force transmission in amoeboid cell migration","date_published":"2009-11-15T00:00:00Z","doi":"10.1038/ncb1992","year":"2009","month":"11"}]