---
_id: '12747'
abstract:
- lang: eng
  text: Muscle degeneration is the most prevalent cause for frailty and dependency
    in inherited diseases and ageing. Elucidation of pathophysiological mechanisms,
    as well as effective treatments for muscle diseases, represents an important goal
    in improving human health. Here, we show that the lipid synthesis enzyme phosphatidylethanolamine
    cytidyltransferase (PCYT2/ECT) is critical to muscle health. Human deficiency
    in PCYT2 causes a severe disease with failure to thrive and progressive weakness.
    pcyt2-mutant zebrafish and muscle-specific Pcyt2-knockout mice recapitulate the
    participant phenotypes, with failure to thrive, progressive muscle weakness and
    accelerated ageing. Mechanistically, muscle Pcyt2 deficiency affects cellular
    bioenergetics and membrane lipid bilayer structure and stability. PCYT2 activity
    declines in ageing muscles of mice and humans, and adeno-associated virus-based
    delivery of PCYT2 ameliorates muscle weakness in Pcyt2-knockout and old mice,
    offering a therapy for individuals with a rare disease and muscle ageing. Thus,
    PCYT2 plays a fundamental and conserved role in vertebrate muscle health, linking
    PCYT2 and PCYT2-synthesized lipids to severe muscle dystrophy and ageing.
acknowledgement: 'The authors thank the participants and their families for participating
  in the study. We thank all members of our laboratories for helpful discussions.
  We are grateful to Vienna BioCenter Core Facilities: Mouse Phenotyping Unit, Histopathology
  Unit, Bioinformatics Unit, BioOptics Unit, Electron Microscopy Unit and Comparative
  Medicine Unit. We are grateful to the Lipidomics Facility, and K. Klavins and T.
  Hannich at the CeMM Research Center for Molecular Medicine of the Austrian Academy
  of Sciences for assistance with lipidomics analysis. We also thank T. Huan and A.
  Hui (UBC Vancouver) for mouse tissue and mitochondria lipidomics analysis. We thank
  A. Klymchenko (Laboratoire de Bioimagerie et Pathologies Université de Strasbourg,
  Strasbourg, France) for providing the NR12S probe. We are thankful to the Sen. Paul
  D. Wellstone Muscular Dystrophy Cooperative Specialized Research Center Viral Vector
  Core Facility for AAV6 production. We also thank K. P. Campbell and M. E. Anderson
  (University of Iowa, Carver College of Medicine) for advice on muscle tissue handling.
  We thank A. Al-Qassabi from the Sultan Qaboos University for the clinical assessment
  of the participants. D.C. and J.M.P. are supported by the Austrian Federal Ministry
  of Education, Science and Research, the Austrian Academy of Sciences, and the City
  of Vienna, and grants from the Austrian Science Fund (FWF) Wittgenstein award (Z
  271-B19), the T. von Zastrow Foundation, and a Canada 150 Research Chairs Program
  (F18-01336). J.S.C. is supported by grants RO1AR44533 and P50AR065139 from the US
  National Institutes of Health. C.K. is supported by a grant from the Agence Nationale
  de la Recherche (ANR-18-CE14-0007-01). A.V.K. is supported by European Union’s Horizon
  2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement
  no. 67544, and an Austrian Science Fund (FWF; no P-33799). A.W. is supported by
  Austrian Research Promotion Agency (FFG) project no 867674. E.S. is supported by
  a SciLifeLab fellowship and Karolinska Institutet Foundation Grants. Work in the
  laboratory of G.S.-F. is supported by the Austrian Academy of Sciences, the European
  Research Council (ERC AdG 695214 GameofGates) and the Innovative Medicines Initiative
  2 Joint Undertaking (grant agreement no. 777372, ReSOLUTE). S.B., M.L. and R.Y.
  acknowledge the support of the Spastic Paraplegia Foundation.'
article_processing_charge: No
article_type: original
author:
- first_name: Domagoj
  full_name: Cikes, Domagoj
  last_name: Cikes
- first_name: Kareem
  full_name: Elsayad, Kareem
  last_name: Elsayad
- first_name: Erdinc
  full_name: Sezgin, Erdinc
  last_name: Sezgin
- first_name: Erika
  full_name: Koitai, Erika
  last_name: Koitai
- first_name: Torma
  full_name: Ferenc, Torma
  last_name: Ferenc
- first_name: Michael
  full_name: Orthofer, Michael
  last_name: Orthofer
- first_name: Rebecca
  full_name: Yarwood, Rebecca
  last_name: Yarwood
- first_name: Leonhard X.
  full_name: Heinz, Leonhard X.
  last_name: Heinz
- first_name: Vitaly
  full_name: Sedlyarov, Vitaly
  last_name: Sedlyarov
- first_name: Nasser
  full_name: Darwish-Miranda, Nasser
  id: 39CD9926-F248-11E8-B48F-1D18A9856A87
  last_name: Darwish-Miranda
  orcid: 0000-0002-8821-8236
- first_name: Adrian
  full_name: Taylor, Adrian
  last_name: Taylor
- first_name: Sophie
  full_name: Grapentine, Sophie
  last_name: Grapentine
- first_name: Fathiya
  full_name: al-Murshedi, Fathiya
  last_name: al-Murshedi
- first_name: Anne
  full_name: Abot, Anne
  last_name: Abot
- first_name: Adelheid
  full_name: Weidinger, Adelheid
  last_name: Weidinger
- first_name: Candice
  full_name: Kutchukian, Candice
  last_name: Kutchukian
- first_name: Colline
  full_name: Sanchez, Colline
  last_name: Sanchez
- first_name: Shane J. F.
  full_name: Cronin, Shane J. F.
  last_name: Cronin
- first_name: Maria
  full_name: Novatchkova, Maria
  last_name: Novatchkova
- first_name: Anoop
  full_name: Kavirayani, Anoop
  last_name: Kavirayani
- first_name: Thomas
  full_name: Schuetz, Thomas
  last_name: Schuetz
- first_name: Bernhard
  full_name: Haubner, Bernhard
  last_name: Haubner
- first_name: Lisa
  full_name: Haas, Lisa
  last_name: Haas
- first_name: Astrid
  full_name: Hagelkruys, Astrid
  last_name: Hagelkruys
- first_name: Suzanne
  full_name: Jackowski, Suzanne
  last_name: Jackowski
- first_name: Andrey
  full_name: Kozlov, Andrey
  last_name: Kozlov
- first_name: Vincent
  full_name: Jacquemond, Vincent
  last_name: Jacquemond
- first_name: Claude
  full_name: Knauf, Claude
  last_name: Knauf
- first_name: Giulio
  full_name: Superti-Furga, Giulio
  last_name: Superti-Furga
- first_name: Eric
  full_name: Rullman, Eric
  last_name: Rullman
- first_name: Thomas
  full_name: Gustafsson, Thomas
  last_name: Gustafsson
- first_name: John
  full_name: McDermot, John
  last_name: McDermot
- first_name: Martin
  full_name: Lowe, Martin
  last_name: Lowe
- first_name: Zsolt
  full_name: Radak, Zsolt
  last_name: Radak
- first_name: Jeffrey S.
  full_name: Chamberlain, Jeffrey S.
  last_name: Chamberlain
- first_name: Marica
  full_name: Bakovic, Marica
  last_name: Bakovic
- first_name: Siddharth
  full_name: Banka, Siddharth
  last_name: Banka
- first_name: Josef M.
  full_name: Penninger, Josef M.
  last_name: Penninger
citation:
  ama: Cikes D, Elsayad K, Sezgin E, et al. PCYT2-regulated lipid biosynthesis is
    critical to muscle health and ageing. <i>Nature Metabolism</i>. 2023;5:495-515.
    doi:<a href="https://doi.org/10.1038/s42255-023-00766-2">10.1038/s42255-023-00766-2</a>
  apa: Cikes, D., Elsayad, K., Sezgin, E., Koitai, E., Ferenc, T., Orthofer, M., …
    Penninger, J. M. (2023). PCYT2-regulated lipid biosynthesis is critical to muscle
    health and ageing. <i>Nature Metabolism</i>. Springer Nature. <a href="https://doi.org/10.1038/s42255-023-00766-2">https://doi.org/10.1038/s42255-023-00766-2</a>
  chicago: Cikes, Domagoj, Kareem Elsayad, Erdinc Sezgin, Erika Koitai, Torma Ferenc,
    Michael Orthofer, Rebecca Yarwood, et al. “PCYT2-Regulated Lipid Biosynthesis
    Is Critical to Muscle Health and Ageing.” <i>Nature Metabolism</i>. Springer Nature,
    2023. <a href="https://doi.org/10.1038/s42255-023-00766-2">https://doi.org/10.1038/s42255-023-00766-2</a>.
  ieee: D. Cikes <i>et al.</i>, “PCYT2-regulated lipid biosynthesis is critical to
    muscle health and ageing,” <i>Nature Metabolism</i>, vol. 5. Springer Nature,
    pp. 495–515, 2023.
  ista: Cikes D, Elsayad K, Sezgin E, Koitai E, Ferenc T, Orthofer M, Yarwood R, Heinz
    LX, Sedlyarov V, Darwish-Miranda N, Taylor A, Grapentine S, al-Murshedi F, Abot
    A, Weidinger A, Kutchukian C, Sanchez C, Cronin SJF, Novatchkova M, Kavirayani
    A, Schuetz T, Haubner B, Haas L, Hagelkruys A, Jackowski S, Kozlov A, Jacquemond
    V, Knauf C, Superti-Furga G, Rullman E, Gustafsson T, McDermot J, Lowe M, Radak
    Z, Chamberlain JS, Bakovic M, Banka S, Penninger JM. 2023. PCYT2-regulated lipid
    biosynthesis is critical to muscle health and ageing. Nature Metabolism. 5, 495–515.
  mla: Cikes, Domagoj, et al. “PCYT2-Regulated Lipid Biosynthesis Is Critical to Muscle
    Health and Ageing.” <i>Nature Metabolism</i>, vol. 5, Springer Nature, 2023, pp.
    495–515, doi:<a href="https://doi.org/10.1038/s42255-023-00766-2">10.1038/s42255-023-00766-2</a>.
  short: D. Cikes, K. Elsayad, E. Sezgin, E. Koitai, T. Ferenc, M. Orthofer, R. Yarwood,
    L.X. Heinz, V. Sedlyarov, N. Darwish-Miranda, A. Taylor, S. Grapentine, F. al-Murshedi,
    A. Abot, A. Weidinger, C. Kutchukian, C. Sanchez, S.J.F. Cronin, M. Novatchkova,
    A. Kavirayani, T. Schuetz, B. Haubner, L. Haas, A. Hagelkruys, S. Jackowski, A.
    Kozlov, V. Jacquemond, C. Knauf, G. Superti-Furga, E. Rullman, T. Gustafsson,
    J. McDermot, M. Lowe, Z. Radak, J.S. Chamberlain, M. Bakovic, S. Banka, J.M. Penninger,
    Nature Metabolism 5 (2023) 495–515.
date_created: 2023-03-23T12:58:43Z
date_published: 2023-03-20T00:00:00Z
date_updated: 2023-11-28T07:31:33Z
day: '20'
department:
- _id: Bio
doi: 10.1038/s42255-023-00766-2
external_id:
  isi:
  - '000992064000002'
  pmid:
  - '36941451'
intvolume: '         5'
isi: 1
keyword:
- Cell Biology
- Physiology (medical)
- Endocrinology
- Diabetes and Metabolism
- Internal Medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2022.03.02.482658
month: '03'
oa: 1
oa_version: Preprint
page: 495-515
pmid: 1
publication: Nature Metabolism
publication_identifier:
  issn:
  - 2522-5812
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.1038/s42255-023-00791-1
scopus_import: '1'
status: public
title: PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2023'
...
---
_id: '8582'
abstract:
- lang: eng
  text: "Cell and tissue polarization is fundamental for plant growth and morphogenesis.
    The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial
    for their function in directional auxin transport. The clustering of PIN polar
    cargoes within the plasma membrane has been proposed to be important for the maintenance
    of their polar distribution. However, the more detailed features of PIN clusters
    and the cellular requirements of cargo clustering remain unclear.\r\nHere, we
    characterized PIN clusters in detail by means of multiple advanced microscopy
    and quantification methods, such as 3D quantitative imaging or freeze‐fracture
    replica labeling. The size and aggregation types of PIN clusters were determined
    by electron microscopy at the nanometer level at different polar domains and at
    different developmental stages, revealing a strong preference for clustering at
    the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters
    depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall
    components as well as connections between the cell wall and the plasma membrane.\r\nThis
    study identifies the role of different cellular processes and structures in polar
    cargo clustering and provides initial mechanistic insight into the maintenance
    of polarity in plants and other systems."
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg)
  for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1
  mutant and the complementation lines, and Dr Patricia C. Zambryski (University of
  California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria)
  for assistance with imaging, group members for discussions, Martine De Cock for
  help in preparing the manuscript and Nataliia Gnyliukh for critical reading and
  revision of the manuscript. This project received funding from the European Research
  Council (ERC) under the European Union's Horizon 2020 research and innovation program
  (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y
  Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People
  Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme
  (FP7/2007‐2013) under REA grant agreement no. 291734.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hongjiang
  full_name: Li, Hongjiang
  id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0001-5039-9660
- first_name: Daniel
  full_name: von Wangenheim, Daniel
  id: 49E91952-F248-11E8-B48F-1D18A9856A87
  last_name: von Wangenheim
  orcid: 0000-0002-6862-1247
- first_name: Xixi
  full_name: Zhang, Xixi
  id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
  last_name: Zhang
  orcid: 0000-0001-7048-4627
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Nasser
  full_name: Darwish-Miranda, Nasser
  id: 39CD9926-F248-11E8-B48F-1D18A9856A87
  last_name: Darwish-Miranda
  orcid: 0000-0002-8821-8236
- first_name: Satoshi
  full_name: Naramoto, Satoshi
  last_name: Naramoto
- first_name: Krzysztof T
  full_name: Wabnik, Krzysztof T
  id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
  last_name: Wabnik
  orcid: 0000-0001-7263-0560
- first_name: Riet
  full_name: de Rycke, Riet
  last_name: de Rycke
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Daniel J
  full_name: Gütl, Daniel J
  id: 381929CE-F248-11E8-B48F-1D18A9856A87
  last_name: Gütl
- first_name: Ricardo
  full_name: Tejos, Ricardo
  last_name: Tejos
- first_name: Peter
  full_name: Grones, Peter
  id: 399876EC-F248-11E8-B48F-1D18A9856A87
  last_name: Grones
- first_name: Meiyu
  full_name: Ke, Meiyu
  last_name: Ke
- first_name: Xu
  full_name: Chen, Xu
  id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
  last_name: Chen
- first_name: Jan
  full_name: Dettmer, Jan
  last_name: Dettmer
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar
    cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369.
    doi:<a href="https://doi.org/10.1111/nph.16887">10.1111/nph.16887</a>
  apa: Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto,
    S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in
    Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href="https://doi.org/10.1111/nph.16887">https://doi.org/10.1111/nph.16887</a>
  chicago: Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda,
    Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar
    Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021.
    <a href="https://doi.org/10.1111/nph.16887">https://doi.org/10.1111/nph.16887</a>.
  ieee: H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering
    in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp.
    351–369, 2021.
  ista: Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik
    KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer
    J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis
    thaliana. New Phytologist. 229(1), 351–369.
  mla: Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering
    in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021,
    pp. 351–69, doi:<a href="https://doi.org/10.1111/nph.16887">10.1111/nph.16887</a>.
  short: H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto,
    K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke,
    X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.
date_created: 2020-09-28T08:59:28Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-08-04T11:01:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
- _id: EvBe
doi: 10.1111/nph.16887
ec_funded: 1
external_id:
  isi:
  - '000570187900001'
file:
- access_level: open_access
  checksum: b45621607b4cab97eeb1605ab58e896e
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-04T09:44:17Z
  date_updated: 2021-02-04T09:44:17Z
  file_id: '9084'
  file_name: 2021_NewPhytologist_Li.pdf
  file_size: 4061962
  relation: main_file
  success: 1
file_date_updated: 2021-02-04T09:44:17Z
has_accepted_license: '1'
intvolume: '       229'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 351-369
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
  eissn:
  - '14698137'
  issn:
  - 0028646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 229
year: '2021'
...
---
_id: '6867'
abstract:
- lang: eng
  text: A novel magnetic scratch method achieves repeatability, reproducibility and
    geometric control greater than pipette scratch assays and closely approximating
    the precision of cell exclusion assays while inducing the cell injury inherently
    necessary for wound healing assays. The magnetic scratch is affordable, easily
    implemented and standardisable and thus may contribute toward better comparability
    of data generated in different studies and laboratories.
article_number: '12625'
article_processing_charge: No
author:
- first_name: M.
  full_name: Fenu, M.
  last_name: Fenu
- first_name: T.
  full_name: Bettermann, T.
  last_name: Bettermann
- first_name: C.
  full_name: Vogl, C.
  last_name: Vogl
- first_name: Nasser
  full_name: Darwish-Miranda, Nasser
  id: 39CD9926-F248-11E8-B48F-1D18A9856A87
  last_name: Darwish-Miranda
  orcid: 0000-0002-8821-8236
- first_name: J.
  full_name: Schramel, J.
  last_name: Schramel
- first_name: F.
  full_name: Jenner, F.
  last_name: Jenner
- first_name: I.
  full_name: Ribitsch, I.
  last_name: Ribitsch
citation:
  ama: Fenu M, Bettermann T, Vogl C, et al. A novel magnet-based scratch method for
    standardisation of wound-healing assays. <i>Scientific Reports</i>. 2019;9(1).
    doi:<a href="https://doi.org/10.1038/s41598-019-48930-7">10.1038/s41598-019-48930-7</a>
  apa: Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner,
    F., &#38; Ribitsch, I. (2019). A novel magnet-based scratch method for standardisation
    of wound-healing assays. <i>Scientific Reports</i>. Springer Nature. <a href="https://doi.org/10.1038/s41598-019-48930-7">https://doi.org/10.1038/s41598-019-48930-7</a>
  chicago: Fenu, M., T. Bettermann, C. Vogl, Nasser Darwish-Miranda, J. Schramel,
    F. Jenner, and I. Ribitsch. “A Novel Magnet-Based Scratch Method for Standardisation
    of Wound-Healing Assays.” <i>Scientific Reports</i>. Springer Nature, 2019. <a
    href="https://doi.org/10.1038/s41598-019-48930-7">https://doi.org/10.1038/s41598-019-48930-7</a>.
  ieee: M. Fenu <i>et al.</i>, “A novel magnet-based scratch method for standardisation
    of wound-healing assays,” <i>Scientific Reports</i>, vol. 9, no. 1. Springer Nature,
    2019.
  ista: Fenu M, Bettermann T, Vogl C, Darwish-Miranda N, Schramel J, Jenner F, Ribitsch
    I. 2019. A novel magnet-based scratch method for standardisation of wound-healing
    assays. Scientific Reports. 9(1), 12625.
  mla: Fenu, M., et al. “A Novel Magnet-Based Scratch Method for Standardisation of
    Wound-Healing Assays.” <i>Scientific Reports</i>, vol. 9, no. 1, 12625, Springer
    Nature, 2019, doi:<a href="https://doi.org/10.1038/s41598-019-48930-7">10.1038/s41598-019-48930-7</a>.
  short: M. Fenu, T. Bettermann, C. Vogl, N. Darwish-Miranda, J. Schramel, F. Jenner,
    I. Ribitsch, Scientific Reports 9 (2019).
date_created: 2019-09-15T22:00:42Z
date_published: 2019-09-02T00:00:00Z
date_updated: 2023-08-29T07:55:15Z
day: '02'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1038/s41598-019-48930-7
external_id:
  isi:
  - '000483697800007'
  pmid:
  - '31477739'
file:
- access_level: open_access
  checksum: 9cfd986d4108e288cc72276ef047ab0c
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publication: Scientific Reports
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title: A novel magnet-based scratch method for standardisation of wound-healing assays
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volume: 9
year: '2019'
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