[{"external_id":{"pmid":["36941451"],"isi":["000992064000002"]},"title":"PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing","doi":"10.1038/s42255-023-00766-2","year":"2023","related_material":{"link":[{"url":"https://doi.org/10.1038/s42255-023-00791-1","relation":"erratum"}]},"isi":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2022.03.02.482658"}],"abstract":[{"lang":"eng","text":"Muscle degeneration is the most prevalent cause for frailty and dependency in inherited diseases and ageing. Elucidation of pathophysiological mechanisms, as well as effective treatments for muscle diseases, represents an important goal in improving human health. Here, we show that the lipid synthesis enzyme phosphatidylethanolamine cytidyltransferase (PCYT2/ECT) is critical to muscle health. Human deficiency in PCYT2 causes a severe disease with failure to thrive and progressive weakness. pcyt2-mutant zebrafish and muscle-specific Pcyt2-knockout mice recapitulate the participant phenotypes, with failure to thrive, progressive muscle weakness and accelerated ageing. Mechanistically, muscle Pcyt2 deficiency affects cellular bioenergetics and membrane lipid bilayer structure and stability. PCYT2 activity declines in ageing muscles of mice and humans, and adeno-associated virus-based delivery of PCYT2 ameliorates muscle weakness in Pcyt2-knockout and old mice, offering a therapy for individuals with a rare disease and muscle ageing. Thus, PCYT2 plays a fundamental and conserved role in vertebrate muscle health, linking PCYT2 and PCYT2-synthesized lipids to severe muscle dystrophy and ageing."}],"author":[{"last_name":"Cikes","full_name":"Cikes, Domagoj","first_name":"Domagoj"},{"full_name":"Elsayad, Kareem","last_name":"Elsayad","first_name":"Kareem"},{"last_name":"Sezgin","full_name":"Sezgin, Erdinc","first_name":"Erdinc"},{"last_name":"Koitai","full_name":"Koitai, Erika","first_name":"Erika"},{"full_name":"Ferenc, Torma","last_name":"Ferenc","first_name":"Torma"},{"last_name":"Orthofer","full_name":"Orthofer, Michael","first_name":"Michael"},{"last_name":"Yarwood","full_name":"Yarwood, Rebecca","first_name":"Rebecca"},{"last_name":"Heinz","full_name":"Heinz, Leonhard X.","first_name":"Leonhard X."},{"first_name":"Vitaly","full_name":"Sedlyarov, Vitaly","last_name":"Sedlyarov"},{"first_name":"Nasser","orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda","id":"39CD9926-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Adrian","last_name":"Taylor","full_name":"Taylor, Adrian"},{"first_name":"Sophie","full_name":"Grapentine, Sophie","last_name":"Grapentine"},{"last_name":"al-Murshedi","full_name":"al-Murshedi, Fathiya","first_name":"Fathiya"},{"first_name":"Anne","last_name":"Abot","full_name":"Abot, Anne"},{"full_name":"Weidinger, Adelheid","last_name":"Weidinger","first_name":"Adelheid"},{"last_name":"Kutchukian","full_name":"Kutchukian, Candice","first_name":"Candice"},{"last_name":"Sanchez","full_name":"Sanchez, Colline","first_name":"Colline"},{"first_name":"Shane J. F.","last_name":"Cronin","full_name":"Cronin, Shane J. F."},{"last_name":"Novatchkova","full_name":"Novatchkova, Maria","first_name":"Maria"},{"full_name":"Kavirayani, Anoop","last_name":"Kavirayani","first_name":"Anoop"},{"full_name":"Schuetz, Thomas","last_name":"Schuetz","first_name":"Thomas"},{"last_name":"Haubner","full_name":"Haubner, Bernhard","first_name":"Bernhard"},{"first_name":"Lisa","last_name":"Haas","full_name":"Haas, Lisa"},{"first_name":"Astrid","last_name":"Hagelkruys","full_name":"Hagelkruys, Astrid"},{"last_name":"Jackowski","full_name":"Jackowski, Suzanne","first_name":"Suzanne"},{"first_name":"Andrey","last_name":"Kozlov","full_name":"Kozlov, Andrey"},{"first_name":"Vincent","full_name":"Jacquemond, Vincent","last_name":"Jacquemond"},{"first_name":"Claude","full_name":"Knauf, Claude","last_name":"Knauf"},{"first_name":"Giulio","full_name":"Superti-Furga, Giulio","last_name":"Superti-Furga"},{"first_name":"Eric","full_name":"Rullman, Eric","last_name":"Rullman"},{"last_name":"Gustafsson","full_name":"Gustafsson, Thomas","first_name":"Thomas"},{"full_name":"McDermot, John","last_name":"McDermot","first_name":"John"},{"first_name":"Martin","full_name":"Lowe, Martin","last_name":"Lowe"},{"last_name":"Radak","full_name":"Radak, Zsolt","first_name":"Zsolt"},{"last_name":"Chamberlain","full_name":"Chamberlain, Jeffrey S.","first_name":"Jeffrey S."},{"first_name":"Marica","last_name":"Bakovic","full_name":"Bakovic, Marica"},{"last_name":"Banka","full_name":"Banka, Siddharth","first_name":"Siddharth"},{"first_name":"Josef M.","full_name":"Penninger, Josef M.","last_name":"Penninger"}],"keyword":["Cell Biology","Physiology (medical)","Endocrinology","Diabetes and Metabolism","Internal Medicine"],"publication_status":"published","citation":{"mla":"Cikes, Domagoj, et al. “PCYT2-Regulated Lipid Biosynthesis Is Critical to Muscle Health and Ageing.” <i>Nature Metabolism</i>, vol. 5, Springer Nature, 2023, pp. 495–515, doi:<a href=\"https://doi.org/10.1038/s42255-023-00766-2\">10.1038/s42255-023-00766-2</a>.","ama":"Cikes D, Elsayad K, Sezgin E, et al. PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. <i>Nature Metabolism</i>. 2023;5:495-515. doi:<a href=\"https://doi.org/10.1038/s42255-023-00766-2\">10.1038/s42255-023-00766-2</a>","short":"D. Cikes, K. Elsayad, E. Sezgin, E. Koitai, T. Ferenc, M. Orthofer, R. Yarwood, L.X. Heinz, V. Sedlyarov, N. Darwish-Miranda, A. Taylor, S. Grapentine, F. al-Murshedi, A. Abot, A. Weidinger, C. Kutchukian, C. Sanchez, S.J.F. Cronin, M. Novatchkova, A. Kavirayani, T. Schuetz, B. Haubner, L. Haas, A. Hagelkruys, S. Jackowski, A. Kozlov, V. Jacquemond, C. Knauf, G. Superti-Furga, E. Rullman, T. Gustafsson, J. McDermot, M. Lowe, Z. Radak, J.S. Chamberlain, M. Bakovic, S. Banka, J.M. Penninger, Nature Metabolism 5 (2023) 495–515.","ista":"Cikes D, Elsayad K, Sezgin E, Koitai E, Ferenc T, Orthofer M, Yarwood R, Heinz LX, Sedlyarov V, Darwish-Miranda N, Taylor A, Grapentine S, al-Murshedi F, Abot A, Weidinger A, Kutchukian C, Sanchez C, Cronin SJF, Novatchkova M, Kavirayani A, Schuetz T, Haubner B, Haas L, Hagelkruys A, Jackowski S, Kozlov A, Jacquemond V, Knauf C, Superti-Furga G, Rullman E, Gustafsson T, McDermot J, Lowe M, Radak Z, Chamberlain JS, Bakovic M, Banka S, Penninger JM. 2023. PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. Nature Metabolism. 5, 495–515.","apa":"Cikes, D., Elsayad, K., Sezgin, E., Koitai, E., Ferenc, T., Orthofer, M., … Penninger, J. M. (2023). PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. <i>Nature Metabolism</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42255-023-00766-2\">https://doi.org/10.1038/s42255-023-00766-2</a>","ieee":"D. Cikes <i>et al.</i>, “PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing,” <i>Nature Metabolism</i>, vol. 5. Springer Nature, pp. 495–515, 2023.","chicago":"Cikes, Domagoj, Kareem Elsayad, Erdinc Sezgin, Erika Koitai, Torma Ferenc, Michael Orthofer, Rebecca Yarwood, et al. “PCYT2-Regulated Lipid Biosynthesis Is Critical to Muscle Health and Ageing.” <i>Nature Metabolism</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s42255-023-00766-2\">https://doi.org/10.1038/s42255-023-00766-2</a>."},"pmid":1,"_id":"12747","publication_identifier":{"issn":["2522-5812"]},"acknowledgement":"The authors thank the participants and their families for participating in the study. We thank all members of our laboratories for helpful discussions. We are grateful to Vienna BioCenter Core Facilities: Mouse Phenotyping Unit, Histopathology Unit, Bioinformatics Unit, BioOptics Unit, Electron Microscopy Unit and Comparative Medicine Unit. We are grateful to the Lipidomics Facility, and K. Klavins and T. Hannich at the CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences for assistance with lipidomics analysis. We also thank T. Huan and A. Hui (UBC Vancouver) for mouse tissue and mitochondria lipidomics analysis. We thank A. Klymchenko (Laboratoire de Bioimagerie et Pathologies Université de Strasbourg, Strasbourg, France) for providing the NR12S probe. We are thankful to the Sen. Paul D. Wellstone Muscular Dystrophy Cooperative Specialized Research Center Viral Vector Core Facility for AAV6 production. We also thank K. P. Campbell and M. E. Anderson (University of Iowa, Carver College of Medicine) for advice on muscle tissue handling. We thank A. Al-Qassabi from the Sultan Qaboos University for the clinical assessment of the participants. D.C. and J.M.P. are supported by the Austrian Federal Ministry of Education, Science and Research, the Austrian Academy of Sciences, and the City of Vienna, and grants from the Austrian Science Fund (FWF) Wittgenstein award (Z 271-B19), the T. von Zastrow Foundation, and a Canada 150 Research Chairs Program (F18-01336). J.S.C. is supported by grants RO1AR44533 and P50AR065139 from the US National Institutes of Health. C.K. is supported by a grant from the Agence Nationale de la Recherche (ANR-18-CE14-0007-01). A.V.K. is supported by European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 67544, and an Austrian Science Fund (FWF; no P-33799). A.W. is supported by Austrian Research Promotion Agency (FFG) project no 867674. E.S. is supported by a SciLifeLab fellowship and Karolinska Institutet Foundation Grants. Work in the laboratory of G.S.-F. is supported by the Austrian Academy of Sciences, the European Research Council (ERC AdG 695214 GameofGates) and the Innovative Medicines Initiative 2 Joint Undertaking (grant agreement no. 777372, ReSOLUTE). S.B., M.L. and R.Y. acknowledge the support of the Spastic Paraplegia Foundation.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Preprint","quality_controlled":"1","volume":5,"oa":1,"date_updated":"2023-11-28T07:31:33Z","article_processing_charge":"No","publisher":"Springer Nature","scopus_import":"1","language":[{"iso":"eng"}],"month":"03","article_type":"original","date_published":"2023-03-20T00:00:00Z","date_created":"2023-03-23T12:58:43Z","department":[{"_id":"Bio"}],"intvolume":"         5","status":"public","day":"20","type":"journal_article","publication":"Nature Metabolism","page":"495-515"},{"article_type":"original","date_published":"2021-01-01T00:00:00Z","month":"01","language":[{"iso":"eng"}],"publisher":"Wiley","scopus_import":"1","department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"license":"https://creativecommons.org/licenses/by/4.0/","has_accepted_license":"1","date_created":"2020-09-28T08:59:28Z","file":[{"file_id":"9084","creator":"dernst","relation":"main_file","content_type":"application/pdf","success":1,"access_level":"open_access","date_updated":"2021-02-04T09:44:17Z","checksum":"b45621607b4cab97eeb1605ab58e896e","date_created":"2021-02-04T09:44:17Z","file_size":4061962,"file_name":"2021_NewPhytologist_Li.pdf"}],"type":"journal_article","day":"01","status":"public","intvolume":"       229","page":"351-369","file_date_updated":"2021-02-04T09:44:17Z","issue":"1","publication":"New Phytologist","ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"}],"year":"2021","doi":"10.1111/nph.16887","external_id":{"isi":["000570187900001"]},"title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["580"],"publication_status":"published","citation":{"apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>.","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>","short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369."},"author":[{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660","first_name":"Hongjiang"},{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"von Wangenheim","full_name":"von Wangenheim, Daniel","orcid":"0000-0002-6862-1247"},{"id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","first_name":"Xixi","orcid":"0000-0001-7048-4627","last_name":"Zhang","full_name":"Zhang, Xixi"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","full_name":"Tan, Shutang","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"id":"39CD9926-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda","first_name":"Nasser"},{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"full_name":"Wabnik, Krzysztof T","last_name":"Wabnik","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"de Rycke, Riet","last_name":"de Rycke","first_name":"Riet"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315"},{"first_name":"Daniel J","last_name":"Gütl","full_name":"Gütl, Daniel J","id":"381929CE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","full_name":"Grones, Peter","first_name":"Peter"},{"last_name":"Ke","full_name":"Ke, Meiyu","first_name":"Meiyu"},{"id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu","last_name":"Chen","full_name":"Chen, Xu"},{"first_name":"Jan","full_name":"Dettmer, Jan","last_name":"Dettmer"},{"last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.","lang":"eng"}],"date_updated":"2023-08-04T11:01:21Z","volume":229,"oa":1,"article_processing_charge":"Yes (via OA deal)","acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"oa_version":"Published Version","_id":"8582","publication_identifier":{"eissn":["14698137"],"issn":["0028646X"]}},{"title":"A novel magnet-based scratch method for standardisation of wound-healing assays","external_id":{"isi":["000483697800007"],"pmid":["31477739"]},"doi":"10.1038/s41598-019-48930-7","year":"2019","ddc":["570"],"article_number":"12625","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"author":[{"first_name":"M.","last_name":"Fenu","full_name":"Fenu, M."},{"first_name":"T.","full_name":"Bettermann, T.","last_name":"Bettermann"},{"first_name":"C.","last_name":"Vogl","full_name":"Vogl, C."},{"first_name":"Nasser","orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda","id":"39CD9926-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schramel, J.","last_name":"Schramel","first_name":"J."},{"full_name":"Jenner, F.","last_name":"Jenner","first_name":"F."},{"full_name":"Ribitsch, I.","last_name":"Ribitsch","first_name":"I."}],"abstract":[{"text":"A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.","lang":"eng"}],"citation":{"mla":"Fenu, M., et al. “A Novel Magnet-Based Scratch Method for Standardisation of Wound-Healing Assays.” <i>Scientific Reports</i>, vol. 9, no. 1, 12625, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41598-019-48930-7\">10.1038/s41598-019-48930-7</a>.","ama":"Fenu M, Bettermann T, Vogl C, et al. A novel magnet-based scratch method for standardisation of wound-healing assays. <i>Scientific Reports</i>. 2019;9(1). doi:<a href=\"https://doi.org/10.1038/s41598-019-48930-7\">10.1038/s41598-019-48930-7</a>","ista":"Fenu M, Bettermann T, Vogl C, Darwish-Miranda N, Schramel J, Jenner F, Ribitsch I. 2019. A novel magnet-based scratch method for standardisation of wound-healing assays. Scientific Reports. 9(1), 12625.","short":"M. Fenu, T. Bettermann, C. Vogl, N. Darwish-Miranda, J. Schramel, F. Jenner, I. Ribitsch, Scientific Reports 9 (2019).","ieee":"M. Fenu <i>et al.</i>, “A novel magnet-based scratch method for standardisation of wound-healing assays,” <i>Scientific Reports</i>, vol. 9, no. 1. Springer Nature, 2019.","apa":"Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner, F., &#38; Ribitsch, I. (2019). A novel magnet-based scratch method for standardisation of wound-healing assays. <i>Scientific Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41598-019-48930-7\">https://doi.org/10.1038/s41598-019-48930-7</a>","chicago":"Fenu, M., T. Bettermann, C. Vogl, Nasser Darwish-Miranda, J. Schramel, F. Jenner, and I. Ribitsch. “A Novel Magnet-Based Scratch Method for Standardisation of Wound-Healing Assays.” <i>Scientific Reports</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41598-019-48930-7\">https://doi.org/10.1038/s41598-019-48930-7</a>."},"publication_status":"published","oa_version":"Published Version","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"eissn":["20452322"]},"pmid":1,"_id":"6867","article_processing_charge":"No","date_updated":"2023-08-29T07:55:15Z","volume":9,"oa":1,"language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Springer Nature","date_published":"2019-09-02T00:00:00Z","month":"09","date_created":"2019-09-15T22:00:42Z","file":[{"file_size":3523795,"file_name":"2019_ScientificReports_Fenu.pdf","checksum":"9cfd986d4108e288cc72276ef047ab0c","date_created":"2019-09-16T12:42:40Z","access_level":"open_access","date_updated":"2020-07-14T12:47:42Z","relation":"main_file","content_type":"application/pdf","creator":"dernst","file_id":"6879"}],"department":[{"_id":"Bio"}],"has_accepted_license":"1","status":"public","intvolume":"         9","type":"journal_article","day":"02","file_date_updated":"2020-07-14T12:47:42Z","publication":"Scientific Reports","issue":"1"}]