[{"type":"journal_article","date_updated":"2023-08-03T12:01:01Z","_id":"11588","publisher":"Ferrata Storti Foundation","doi":"10.3324/haematol.2021.278896","article_processing_charge":"No","quality_controlled":"1","ddc":["570"],"page":"1669-1680","external_id":{"isi":["000823746100018"]},"year":"2022","isi":1,"date_published":"2022-07-01T00:00:00Z","acknowledgement":"This study was supported by the Deutsche Forschungsgemeinschaft (DFG) SFB 914 ( to SM [B02 and Z01]), the DFG SFB 1123 (to SM [B06]), the DFG FOR 2033 (to SM), the German\r\nCenter for Cardiovascular Research (DZHK) (Clinician Scientist Programme), MHA 1.4VD (to SM), Postdoc Start-up Grant, 81X3600213 (to FG), 81X3600222 (to LN), the FP7 program\r\n(project 260309, PRESTIGE [to SM]). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 83344, ERC-2018-ADG “IMMUNOTHROMBOSIS” [to SM] and the Marie Skłodowska Curie Individual Fellowship (EU project 747687, LamelliActin [to FG]). ","ec_funded":1,"project":[{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"status":"public","publication":"Haematologica","article_type":"original","date_created":"2022-07-17T22:01:54Z","volume":107,"oa_version":"Published Version","title":"Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo","author":[{"first_name":"Leo","full_name":"Nicolai, Leo","last_name":"Nicolai"},{"first_name":"Rainer","last_name":"Kaiser","full_name":"Kaiser, Rainer"},{"first_name":"Raphael","last_name":"Escaig","full_name":"Escaig, Raphael"},{"last_name":"Hoffknecht","full_name":"Hoffknecht, Marie Louise","first_name":"Marie Louise"},{"full_name":"Anjum, Afra","last_name":"Anjum","first_name":"Afra"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"full_name":"Pircher, Joachim","last_name":"Pircher","first_name":"Joachim"},{"last_name":"Ehrlich","full_name":"Ehrlich, Andreas","first_name":"Andreas"},{"full_name":"Lorenz, Michael","last_name":"Lorenz","first_name":"Michael"},{"last_name":"Ishikawa-Ankerhold","full_name":"Ishikawa-Ankerhold, Hellen","first_name":"Hellen"},{"first_name":"William C.","full_name":"Aird, William C.","last_name":"Aird"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","last_name":"Gärtner","orcid":"0000-0001-6120-3723","first_name":"Florian R"}],"day":"01","scopus_import":"1","publication_status":"published","publication_identifier":{"issn":["0390-6078"],"eissn":["1592-8721"]},"file_date_updated":"2022-07-18T07:51:55Z","intvolume":"       107","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"abstract":[{"text":"Visualizing cell behavior and effector function on a single cell level has been crucial for understanding key aspects of mammalian biology. Due to their small size, large number and rapid recruitment into thrombi, there is a lack of data on fate and behavior of individual platelets in thrombosis and hemostasis. Here we report the use of platelet lineage restricted multi-color reporter mouse strains to delineate platelet function on a single cell level. We show that genetic labeling allows for single platelet and megakaryocyte (MK) tracking and morphological analysis in vivo and in vitro, while not affecting lineage functions. Using Cre-driven Confetti expression, we provide insights into temporal gene expression patterns as well as spatial clustering of MK in the bone marrow. In the vasculature, shape analysis of activated platelets recruited to thrombi identifies ubiquitous filopodia formation with no evidence of lamellipodia formation. Single cell tracking in complex thrombi reveals prominent myosin-dependent motility of platelets and highlights thrombus formation as a highly dynamic process amenable to modification and intervention of the acto-myosin cytoskeleton. Platelet function assays combining flow cytrometry, as well as in vivo, ex vivo and in vitro imaging show unaltered platelet functions of multicolor reporter mice compared to wild-type controls. In conclusion, platelet lineage multicolor reporter mice prove useful in furthering our understanding of platelet and MK biology on a single cell level.","lang":"eng"}],"has_accepted_license":"1","file":[{"content_type":"application/pdf","access_level":"open_access","file_name":"2022_Haematologica_Nicolai.pdf","success":1,"checksum":"9b47830945f3c30428fe9cfee2dc4a8a","relation":"main_file","creator":"dernst","date_updated":"2022-07-18T07:51:55Z","date_created":"2022-07-18T07:51:55Z","file_size":1722094,"file_id":"11595"}],"department":[{"_id":"MiSi"}],"month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"7","citation":{"mla":"Nicolai, Leo, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>, vol. 107, no. 7, Ferrata Storti Foundation, 2022, pp. 1669–80, doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>.","apa":"Nicolai, L., Kaiser, R., Escaig, R., Hoffknecht, M. L., Anjum, A., Leunig, A., … Gärtner, F. R. (2022). Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. Ferrata Storti Foundation. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>","chicago":"Nicolai, Leo, Rainer Kaiser, Raphael Escaig, Marie Louise Hoffknecht, Afra Anjum, Alexander Leunig, Joachim Pircher, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>. Ferrata Storti Foundation, 2022. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>.","ista":"Nicolai L, Kaiser R, Escaig R, Hoffknecht ML, Anjum A, Leunig A, Pircher J, Ehrlich A, Lorenz M, Ishikawa-Ankerhold H, Aird WC, Massberg S, Gärtner FR. 2022. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. Haematologica. 107(7), 1669–1680.","short":"L. Nicolai, R. Kaiser, R. Escaig, M.L. Hoffknecht, A. Anjum, A. Leunig, J. Pircher, A. Ehrlich, M. Lorenz, H. Ishikawa-Ankerhold, W.C. Aird, S. Massberg, F.R. Gärtner, Haematologica 107 (2022) 1669–1680.","ieee":"L. Nicolai <i>et al.</i>, “Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo,” <i>Haematologica</i>, vol. 107, no. 7. Ferrata Storti Foundation, pp. 1669–1680, 2022.","ama":"Nicolai L, Kaiser R, Escaig R, et al. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. 2022;107(7):1669-1680. doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>"},"language":[{"iso":"eng"}],"oa":1},{"external_id":{"isi":["000922019600003"],"pmid":["36272416"]},"year":"2022","isi":1,"keyword":["Infectious Diseases","Immunology","Immunology and Allergy"],"status":"public","publication":"Immunity","project":[{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"acknowledgement":"We thank Coung Kieu and Dominik van den Heuvel for excellent technical assistance. This work was supported by the German Research Foundation (PE2704/2-1, PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR 332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12 to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon 2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G. was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’ by the German Federal\r\nMinistry of Education and Research to the German Center for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care through the research project DigiMed Bayern.","date_published":"2022-12-13T00:00:00Z","ec_funded":1,"pmid":1,"publisher":"Elsevier","article_processing_charge":"No","doi":"10.1016/j.immuni.2022.10.001","type":"journal_article","_id":"12119","date_updated":"2023-08-03T14:21:51Z","ddc":["570"],"page":"2285-2299.e7","quality_controlled":"1","month":"12","file":[{"checksum":"073267a9c0ad9f85a650053bc7b23777","relation":"main_file","access_level":"open_access","content_type":"application/pdf","success":1,"file_name":"2022_Immunity_Petzold.pdf","file_id":"12341","creator":"dernst","date_updated":"2023-01-23T10:18:48Z","file_size":5299475,"date_created":"2023-01-23T10:18:48Z"}],"department":[{"_id":"MiSi"}],"language":[{"iso":"eng"}],"oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"short":"T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L. Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S. Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach, E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian, F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity 55 (2022) 2285–2299.e7.","ieee":"T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol. 55, no. 12. Elsevier, p. 2285–2299.e7, 2022.","ama":"Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. 2022;55(12):2285-2299.e7. doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>","mla":"Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12, Elsevier, 2022, p. 2285–2299.e7, doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>.","apa":"Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M., … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>","chicago":"Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin, Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>.","ista":"Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K, Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. Immunity. 55(12), 2285–2299.e7."},"issue":"12","title":"Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease","oa_version":"Published Version","scopus_import":"1","day":"13","author":[{"full_name":"Petzold, Tobias","last_name":"Petzold","first_name":"Tobias"},{"full_name":"Zhang, Zhe","last_name":"Zhang","first_name":"Zhe"},{"first_name":"Iván","full_name":"Ballesteros, Iván","last_name":"Ballesteros"},{"last_name":"Saleh","full_name":"Saleh, Inas","first_name":"Inas"},{"first_name":"Amin","last_name":"Polzin","full_name":"Polzin, Amin"},{"last_name":"Thienel","full_name":"Thienel, Manuela","first_name":"Manuela"},{"first_name":"Lulu","full_name":"Liu, Lulu","last_name":"Liu"},{"first_name":"Qurrat","last_name":"Ul Ain","full_name":"Ul Ain, Qurrat"},{"full_name":"Ehreiser, Vincent","last_name":"Ehreiser","first_name":"Vincent"},{"first_name":"Christian","full_name":"Weber, Christian","last_name":"Weber"},{"last_name":"Kilani","full_name":"Kilani, Badr","first_name":"Badr"},{"full_name":"Mertsch, Pontus","last_name":"Mertsch","first_name":"Pontus"},{"first_name":"Jeremias","last_name":"Götschke","full_name":"Götschke, Jeremias"},{"full_name":"Cremer, Sophie","last_name":"Cremer","first_name":"Sophie"},{"full_name":"Fu, Wenwen","last_name":"Fu","first_name":"Wenwen"},{"full_name":"Lorenz, Michael","last_name":"Lorenz","first_name":"Michael"},{"first_name":"Hellen","full_name":"Ishikawa-Ankerhold, Hellen","last_name":"Ishikawa-Ankerhold"},{"first_name":"Elisabeth","full_name":"Raatz, Elisabeth","last_name":"Raatz"},{"full_name":"El-Nemr, Shaza","last_name":"El-Nemr","first_name":"Shaza"},{"last_name":"Görlach","full_name":"Görlach, Agnes","first_name":"Agnes"},{"first_name":"Esther","full_name":"Marhuenda, Esther","last_name":"Marhuenda"},{"first_name":"Konstantin","full_name":"Stark, Konstantin","last_name":"Stark"},{"last_name":"Pircher","full_name":"Pircher, Joachim","first_name":"Joachim"},{"last_name":"Stegner","full_name":"Stegner, David","first_name":"David"},{"first_name":"Christian","full_name":"Gieger, Christian","last_name":"Gieger"},{"first_name":"Marc","full_name":"Schmidt-Supprian, Marc","last_name":"Schmidt-Supprian"},{"full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","first_name":"Florian R","orcid":"0000-0001-6120-3723"},{"last_name":"Almendros","full_name":"Almendros, Isaac","first_name":"Isaac"},{"full_name":"Kelm, Malte","last_name":"Kelm","first_name":"Malte"},{"first_name":"Christian","last_name":"Schulz","full_name":"Schulz, Christian"},{"first_name":"Andrés","last_name":"Hidalgo","full_name":"Hidalgo, Andrés"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"date_created":"2023-01-12T11:56:54Z","article_type":"original","volume":55,"abstract":[{"lang":"eng","text":"Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species. By these mechanisms, neutrophils accelerate proplatelet growth and facilitate continuous release of platelets in steady state. Following myocardial infarction, plucking neutrophils drove excessive release of young, reticulated platelets and boosted the risk of recurrent ischemia. Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent thrombosis after myocardial infarction and thrombus burden in venous thrombosis. We establish neutrophil plucking as a target to reduce thromboischemic events."}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"intvolume":"        55","has_accepted_license":"1","file_date_updated":"2023-01-23T10:18:48Z","publication_status":"published","publication_identifier":{"issn":["1074-7613"]}},{"scopus_import":"1","day":"22","author":[{"last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","orcid":"0000-0001-6120-3723"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"}],"oa_version":"Published Version","title":"Engaging the front wheels to drive through fibrous terrain","volume":56,"date_created":"2021-03-28T22:01:41Z","article_type":"original","abstract":[{"text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments.","lang":"eng"}],"intvolume":"        56","publication_identifier":{"issn":["15345807"],"eissn":["18781551"]},"publication_status":"published","month":"03","department":[{"_id":"MiSi"}],"oa":1,"language":[{"iso":"eng"}],"citation":{"ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” <i>Developmental Cell</i>, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. 2021;56(6):723-725. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>","apa":"Gärtner, F. R., &#38; Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>","mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>.","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>.","ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725."},"issue":"6","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","doi":"10.1016/j.devcel.2021.03.002","publisher":"Elsevier","_id":"9294","date_updated":"2023-08-07T14:26:47Z","type":"journal_article","page":"723-725","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2021.03.002"}],"quality_controlled":"1","isi":1,"year":"2021","external_id":{"pmid":["33756118"],"isi":["000631681200004"]},"status":"public","publication":"Developmental Cell","pmid":1,"date_published":"2021-03-22T00:00:00Z"},{"department":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"MiSi"}],"month":"06","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ama":"Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental topography. <i>Nature</i>. 2020;582:582–585. doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>","ieee":"A. Reversat <i>et al.</i>, “Cellular locomotion using environmental topography,” <i>Nature</i>, vol. 582. Springer Nature, pp. 582–585, 2020.","short":"A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez, M.K. Sixt, Nature 582 (2020) 582–585.","ista":"Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL, de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK. 2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.","chicago":"Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan, Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>.","apa":"Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>","mla":"Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>, vol. 582, Springer Nature, 2020, pp. 582–585, doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>."},"language":[{"iso":"eng"}],"date_created":"2020-05-24T22:01:01Z","article_type":"original","volume":582,"oa_version":"None","title":"Cellular locomotion using environmental topography","day":"25","scopus_import":"1","author":[{"last_name":"Reversat","full_name":"Reversat, Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0666-8928","first_name":"Anne"},{"orcid":"0000-0001-6120-3723","first_name":"Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","last_name":"Gärtner"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin"},{"first_name":"Julian A","last_name":"Stopp","full_name":"Stopp, Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","first_name":"Saren"},{"first_name":"Juan L","orcid":"0000-0002-2862-8372","full_name":"Aguilera Servin, Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87","last_name":"Aguilera Servin"},{"first_name":"Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"last_name":"Hons","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","first_name":"Miroslav","orcid":"0000-0002-6625-3348"},{"last_name":"Piel","full_name":"Piel, Matthieu","first_name":"Matthieu"},{"first_name":"Andrew","last_name":"Callan-Jones","full_name":"Callan-Jones, Andrew"},{"first_name":"Raphael","last_name":"Voituriez","full_name":"Voituriez, Raphael"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"}],"publication_identifier":{"eissn":["14764687"],"issn":["00280836"]},"publication_status":"published","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"abstract":[{"lang":"eng","text":"Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force coupling is usually mediated by transmembrane adhesion receptors, especially those of the integrin family, amoeboid cells such as leukocytes can migrate extremely fast despite very low adhesive forces1. Here we show that leukocytes cannot only migrate under low adhesion but can also transmit forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographical features of the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating retrograde shear forces that are sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent migration are not mutually exclusive, but rather are variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate interchangeably and simultaneously. As adhesion-free migration is independent of the chemical composition of the environment, it renders cells completely autonomous in their locomotive behaviour."}],"intvolume":"       582","related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/"}],"record":[{"relation":"dissertation_contains","status":"public","id":"14697"},{"id":"12401","relation":"dissertation_contains","status":"public"}]},"external_id":{"isi":["000532688300008"]},"isi":1,"year":"2020","date_published":"2020-06-25T00:00:00Z","acknowledgement":"We thank A. Leithner and J. Renkawitz for discussion and critical reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic setups; the Bioimaging Facility of IST Austria for excellent support, as well as the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan, L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687.","ec_funded":1,"publication":"Nature","status":"public","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"281556","call_identifier":"FP7"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373","call_identifier":"H2020"},{"_id":"26018E70-B435-11E9-9278-68D0E5697425","grant_number":"P29911","name":"Mechanical adaptation of lamellipodial actin","call_identifier":"FWF"},{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687"}],"type":"journal_article","_id":"7885","date_updated":"2024-03-25T23:30:12Z","publisher":"Springer Nature","article_processing_charge":"No","doi":"10.1038/s41586-020-2283-z","quality_controlled":"1","page":"582–585"},{"department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"file":[{"creator":"dernst","date_updated":"2020-11-23T13:29:49Z","file_size":7035340,"date_created":"2020-11-23T13:29:49Z","file_id":"8798","access_level":"open_access","content_type":"application/pdf","success":1,"file_name":"2020_NatureComm_Nicolai.pdf","checksum":"485b7b6cf30198ba0ce126491a28f125","relation":"main_file"}],"article_number":"5778","month":"11","citation":{"mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>, vol. 11, 5778, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>.","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>","ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>.","short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","ieee":"L. Nicolai <i>et al.</i>, “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"language":[{"iso":"eng"}],"volume":11,"date_created":"2020-11-22T23:01:23Z","article_type":"original","scopus_import":"1","day":"13","author":[{"first_name":"Leo","last_name":"Nicolai","full_name":"Nicolai, Leo"},{"full_name":"Schiefelbein, Karin","last_name":"Schiefelbein","first_name":"Karin"},{"first_name":"Silvia","last_name":"Lipsky","full_name":"Lipsky, Silvia"},{"last_name":"Leunig","full_name":"Leunig, Alexander","first_name":"Alexander"},{"last_name":"Hoffknecht","full_name":"Hoffknecht, Marie","first_name":"Marie"},{"first_name":"Kami","full_name":"Pekayvaz, Kami","last_name":"Pekayvaz"},{"first_name":"Ben","full_name":"Raude, Ben","last_name":"Raude"},{"first_name":"Charlotte","full_name":"Marx, Charlotte","last_name":"Marx"},{"full_name":"Ehrlich, Andreas","last_name":"Ehrlich","first_name":"Andreas"},{"first_name":"Joachim","last_name":"Pircher","full_name":"Pircher, Joachim"},{"full_name":"Zhang, Zhe","last_name":"Zhang","first_name":"Zhe"},{"first_name":"Inas","full_name":"Saleh, Inas","last_name":"Saleh"},{"first_name":"Anna-Kristina","last_name":"Marel","full_name":"Marel, Anna-Kristina"},{"first_name":"Achim","last_name":"Löf","full_name":"Löf, Achim"},{"first_name":"Tobias","last_name":"Petzold","full_name":"Petzold, Tobias"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"last_name":"Stark","full_name":"Stark, Konstantin","first_name":"Konstantin"},{"first_name":"Robert","last_name":"Pick","full_name":"Pick, Robert"},{"first_name":"Gerhild","full_name":"Rosenberger, Gerhild","last_name":"Rosenberger"},{"last_name":"Weckbach","full_name":"Weckbach, Ludwig","first_name":"Ludwig"},{"first_name":"Bernd","full_name":"Uhl, Bernd","last_name":"Uhl"},{"first_name":"Sheng","full_name":"Xia, Sheng","last_name":"Xia"},{"full_name":"Reichel, Christoph Andreas","last_name":"Reichel","first_name":"Christoph Andreas"},{"first_name":"Barbara","last_name":"Walzog","full_name":"Walzog, Barbara"},{"first_name":"Christian","last_name":"Schulz","full_name":"Schulz, Christian"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","full_name":"Zheden, Vanessa","last_name":"Zheden","orcid":"0000-0002-9438-4783","first_name":"Vanessa"},{"last_name":"Bender","full_name":"Bender, Markus","first_name":"Markus"},{"first_name":"Rong","full_name":"Li, Rong","last_name":"Li"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"first_name":"Florian R","orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner"}],"oa_version":"Published Version","title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","file_date_updated":"2020-11-23T13:29:49Z","publication_identifier":{"eissn":["20411723"]},"publication_status":"published","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets.","lang":"eng"}],"intvolume":"        11","year":"2020","isi":1,"related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41467-022-31310-7"}]},"external_id":{"isi":["000594648000014"],"pmid":["33188196"]},"ec_funded":1,"pmid":1,"acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687.","date_published":"2020-11-13T00:00:00Z","status":"public","publication":"Nature Communications","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687","call_identifier":"H2020"}],"_id":"8787","date_updated":"2023-08-22T13:26:26Z","type":"journal_article","article_processing_charge":"No","doi":"10.1038/s41467-020-19515-0","publisher":"Springer Nature","quality_controlled":"1","ddc":["570"]},{"oa_version":"None","title":"Patrolling the vascular borders: Platelets in immunity to infection and cancer","day":"01","scopus_import":"1","author":[{"last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","first_name":"Florian R"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"}],"date_created":"2019-08-20T17:24:32Z","article_type":"original","volume":19,"intvolume":"        19","abstract":[{"text":"Platelets are small anucleate cellular fragments that are released by megakaryocytes and safeguard vascular integrity through a process termed ‘haemostasis’. However, platelets have important roles beyond haemostasis as they contribute to the initiation and coordination of intravascular immune responses. They continuously monitor blood vessel integrity and tightly coordinate vascular trafficking and functions of multiple cell types. In this way platelets act as ‘patrolling officers of the vascular highway’ that help to establish effective immune responses to infections and cancer. Here we discuss the distinct biological features of platelets that allow them to shape immune responses to pathogens and tumour cells, highlighting the parallels between these responses.","lang":"eng"}],"publication_status":"published","publication_identifier":{"issn":["1474-1733"],"eissn":["1474-1741"]},"month":"12","department":[{"_id":"MiSi"}],"language":[{"iso":"eng"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Gärtner, Florian R., and Steffen Massberg. “Patrolling the Vascular Borders: Platelets in Immunity to Infection and Cancer.” <i>Nature Reviews Immunology</i>, vol. 19, no. 12, Springer Nature, 2019, pp. 747–760, doi:<a href=\"https://doi.org/10.1038/s41577-019-0202-z\">10.1038/s41577-019-0202-z</a>.","apa":"Gärtner, F. R., &#38; Massberg, S. (2019). Patrolling the vascular borders: Platelets in immunity to infection and cancer. <i>Nature Reviews Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41577-019-0202-z\">https://doi.org/10.1038/s41577-019-0202-z</a>","ista":"Gärtner FR, Massberg S. 2019. Patrolling the vascular borders: Platelets in immunity to infection and cancer. Nature Reviews Immunology. 19(12), 747–760.","chicago":"Gärtner, Florian R, and Steffen Massberg. “Patrolling the Vascular Borders: Platelets in Immunity to Infection and Cancer.” <i>Nature Reviews Immunology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41577-019-0202-z\">https://doi.org/10.1038/s41577-019-0202-z</a>.","short":"F.R. Gärtner, S. Massberg, Nature Reviews Immunology 19 (2019) 747–760.","ieee":"F. R. Gärtner and S. Massberg, “Patrolling the vascular borders: Platelets in immunity to infection and cancer,” <i>Nature Reviews Immunology</i>, vol. 19, no. 12. Springer Nature, pp. 747–760, 2019.","ama":"Gärtner FR, Massberg S. Patrolling the vascular borders: Platelets in immunity to infection and cancer. <i>Nature Reviews Immunology</i>. 2019;19(12):747–760. doi:<a href=\"https://doi.org/10.1038/s41577-019-0202-z\">10.1038/s41577-019-0202-z</a>"},"issue":"12","publisher":"Springer Nature","article_processing_charge":"No","doi":"10.1038/s41577-019-0202-z","type":"journal_article","_id":"6824","date_updated":"2023-08-29T07:16:14Z","page":"747–760","quality_controlled":"1","external_id":{"pmid":["31409920"],"isi":["000499090600011"]},"isi":1,"year":"2019","status":"public","publication":"Nature Reviews Immunology","project":[{"call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"date_published":"2019-12-01T00:00:00Z","ec_funded":1,"pmid":1},{"project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687"}],"status":"public","publication":"Trends in Immunology","pmid":1,"ec_funded":1,"date_published":"2019-10-01T00:00:00Z","year":"2019","isi":1,"external_id":{"pmid":["31601520"],"isi":["000493292100005"]},"page":"922-938","quality_controlled":"1","doi":"10.1016/j.it.2019.08.004","article_processing_charge":"No","publisher":"Cell Press","date_updated":"2023-08-30T07:19:23Z","_id":"6988","type":"journal_article","language":[{"iso":"eng"}],"issue":"10","citation":{"short":"L. Nicolai, F.R. Gärtner, S. Massberg, Trends in Immunology 40 (2019) 922–938.","ieee":"L. Nicolai, F. R. Gärtner, and S. Massberg, “Platelets in host defense: Experimental and clinical insights,” <i>Trends in Immunology</i>, vol. 40, no. 10. Cell Press, pp. 922–938, 2019.","ama":"Nicolai L, Gärtner FR, Massberg S. Platelets in host defense: Experimental and clinical insights. <i>Trends in Immunology</i>. 2019;40(10):922-938. doi:<a href=\"https://doi.org/10.1016/j.it.2019.08.004\">10.1016/j.it.2019.08.004</a>","mla":"Nicolai, Leo, et al. “Platelets in Host Defense: Experimental and Clinical Insights.” <i>Trends in Immunology</i>, vol. 40, no. 10, Cell Press, 2019, pp. 922–38, doi:<a href=\"https://doi.org/10.1016/j.it.2019.08.004\">10.1016/j.it.2019.08.004</a>.","apa":"Nicolai, L., Gärtner, F. R., &#38; Massberg, S. (2019). Platelets in host defense: Experimental and clinical insights. <i>Trends in Immunology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.it.2019.08.004\">https://doi.org/10.1016/j.it.2019.08.004</a>","chicago":"Nicolai, Leo, Florian R Gärtner, and Steffen Massberg. “Platelets in Host Defense: Experimental and Clinical Insights.” <i>Trends in Immunology</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.it.2019.08.004\">https://doi.org/10.1016/j.it.2019.08.004</a>.","ista":"Nicolai L, Gärtner FR, Massberg S. 2019. Platelets in host defense: Experimental and clinical insights. Trends in Immunology. 40(10), 922–938."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"10","department":[{"_id":"MiSi"}],"intvolume":"        40","abstract":[{"lang":"eng","text":"Platelets are central players in thrombosis and hemostasis but are increasingly recognized as key components of the immune system. They shape ensuing immune responses by recruiting leukocytes, and support the development of adaptive immunity. Recent data shed new light on the complex role of platelets in immunity. Here, we summarize experimental and clinical data on the role of platelets in host defense against bacteria. Platelets bind, contain, and kill bacteria directly; however, platelet proinflammatory effector functions and cross-talk with the coagulation system, can also result in damage to the host (e.g., acute lung injury and sepsis). Novel clinical insights support this dichotomy: platelet inhibition/thrombocytopenia can be either harmful or protective, depending on pathophysiological context. Clinical studies are currently addressing this aspect in greater depth."}],"publication_identifier":{"issn":["1471-4906"]},"publication_status":"published","author":[{"last_name":"Nicolai","full_name":"Nicolai, Leo","first_name":"Leo"},{"first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","last_name":"Gärtner"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"scopus_import":"1","day":"01","oa_version":"None","title":"Platelets in host defense: Experimental and clinical insights","volume":40,"article_type":"review","date_created":"2019-11-04T16:27:36Z"},{"ddc":["570"],"quality_controlled":"1","publisher":"Bio-Protocol","doi":"10.21769/bioprotoc.3018","type":"journal_article","_id":"6354","date_updated":"2021-01-12T08:07:12Z","publication":"Bio-Protocol","status":"public","project":[{"call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"acknowledgement":" FöFoLe project 947 (F.G.), the Friedrich-Baur-Stiftung project 41/16 (F.G.)","date_published":"2018-09-20T00:00:00Z","ec_funded":1,"year":"2018","keyword":["Platelets","Cell migration","Bacteria","Shear flow","Fibrinogen","E. coli"],"intvolume":"         8","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing efficient intravascular bacterial trapping. Here, we describe a method that allows analyzing platelet migration in vitro, focusing on their ability to collect bacteria and trap bacteria under flow."}],"has_accepted_license":"1","file_date_updated":"2020-07-14T12:47:28Z","publication_identifier":{"issn":["2331-8325"]},"publication_status":"published","title":"Platelet migration and bacterial trapping assay under flow","oa_version":"Published Version","day":"20","author":[{"first_name":"Shuxia","last_name":"Fan","full_name":"Fan, Shuxia"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"first_name":"Steffen","last_name":"Massberg","full_name":"Massberg, Steffen"},{"orcid":"0000-0001-6120-3723","first_name":"Florian R","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner"}],"date_created":"2019-04-29T09:40:33Z","volume":8,"language":[{"iso":"eng"}],"oa":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Fan S, Lorenz M, Massberg S, Gärtner FR. 2018. Platelet migration and bacterial trapping assay under flow. Bio-Protocol. 8(18), e3018.","chicago":"Fan, Shuxia, Michael Lorenz, Steffen Massberg, and Florian R Gärtner. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>. Bio-Protocol, 2018. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>.","apa":"Fan, S., Lorenz, M., Massberg, S., &#38; Gärtner, F. R. (2018). Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>","mla":"Fan, Shuxia, et al. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>, vol. 8, no. 18, e3018, Bio-Protocol, 2018, doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>.","ama":"Fan S, Lorenz M, Massberg S, Gärtner FR. Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. 2018;8(18). doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>","ieee":"S. Fan, M. Lorenz, S. Massberg, and F. R. Gärtner, “Platelet migration and bacterial trapping assay under flow,” <i>Bio-Protocol</i>, vol. 8, no. 18. Bio-Protocol, 2018.","short":"S. Fan, M. Lorenz, S. Massberg, F.R. Gärtner, Bio-Protocol 8 (2018)."},"issue":"18","month":"09","article_number":"e3018","file":[{"file_name":"2018_BioProtocol_Fan.pdf","access_level":"open_access","content_type":"application/pdf","relation":"main_file","checksum":"d4588377e789da7f360b553ae02c5119","date_created":"2019-04-30T08:04:33Z","file_size":2928337,"creator":"dernst","date_updated":"2020-07-14T12:47:28Z","file_id":"6360"}],"department":[{"_id":"MiSi"}]},{"pmid":1,"ec_funded":1,"acknowledgement":"This work was funded by grants from the European Research Council (ERC StG 281556 and CoG 724373) and the Austrian Science Foundation (FWF) to M.S. and by Swiss National Foundation (SNF) project grants 31003A_135649, 31003A_153457 and CR23I3_156234 to J.V.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687, and J.R. was funded by an EMBO long-term fellowship (ALTF 1396-2014).","date_published":"2018-05-18T00:00:00Z","project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373","call_identifier":"H2020"},{"call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration","_id":"25A48D24-B435-11E9-9278-68D0E5697425"},{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"}],"status":"public","publication":"Nature Immunology","publist_id":"8040","isi":1,"year":"2018","external_id":{"pmid":["29777221"],"isi":["000433041500026"]},"related_material":{"record":[{"id":"6891","status":"public","relation":"dissertation_contains"}]},"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/29777221"}],"quality_controlled":"1","page":"606 - 616","date_updated":"2024-03-25T23:30:22Z","_id":"15","type":"journal_article","doi":"10.1038/s41590-018-0109-z","article_processing_charge":"No","publisher":"Nature Publishing Group","issue":"6","citation":{"ista":"Hons M, Kopf A, Hauschild R, Leithner AF, Gärtner FR, Abe J, Renkawitz J, Stein J, Sixt MK. 2018. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 19(6), 606–616.","chicago":"Hons, Miroslav, Aglaja Kopf, Robert Hauschild, Alexander F Leithner, Florian R Gärtner, Jun Abe, Jörg Renkawitz, Jens Stein, and Michael K Sixt. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” <i>Nature Immunology</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41590-018-0109-z\">https://doi.org/10.1038/s41590-018-0109-z</a>.","apa":"Hons, M., Kopf, A., Hauschild, R., Leithner, A. F., Gärtner, F. R., Abe, J., … Sixt, M. K. (2018). Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. <i>Nature Immunology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41590-018-0109-z\">https://doi.org/10.1038/s41590-018-0109-z</a>","mla":"Hons, Miroslav, et al. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” <i>Nature Immunology</i>, vol. 19, no. 6, Nature Publishing Group, 2018, pp. 606–16, doi:<a href=\"https://doi.org/10.1038/s41590-018-0109-z\">10.1038/s41590-018-0109-z</a>.","ama":"Hons M, Kopf A, Hauschild R, et al. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. <i>Nature Immunology</i>. 2018;19(6):606-616. doi:<a href=\"https://doi.org/10.1038/s41590-018-0109-z\">10.1038/s41590-018-0109-z</a>","ieee":"M. Hons <i>et al.</i>, “Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells,” <i>Nature Immunology</i>, vol. 19, no. 6. Nature Publishing Group, pp. 606–616, 2018.","short":"M. Hons, A. Kopf, R. Hauschild, A.F. Leithner, F.R. Gärtner, J. Abe, J. Renkawitz, J. Stein, M.K. Sixt, Nature Immunology 19 (2018) 606–616."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"language":[{"iso":"eng"}],"department":[{"_id":"MiSi"},{"_id":"Bio"}],"month":"05","publication_status":"published","abstract":[{"lang":"eng","text":"Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux."}],"intvolume":"        19","acknowledged_ssus":[{"_id":"SSU"}],"volume":19,"date_created":"2018-12-11T11:44:10Z","author":[{"first_name":"Miroslav","orcid":"0000-0002-6625-3348","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","last_name":"Hons"},{"full_name":"Kopf, Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","last_name":"Kopf","first_name":"Aglaja","orcid":"0000-0002-2187-6656"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"full_name":"Leithner, Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","last_name":"Leithner","first_name":"Alexander F","orcid":"0000-0002-1073-744X"},{"first_name":"Florian R","orcid":"0000-0001-6120-3723","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R"},{"first_name":"Jun","full_name":"Abe, Jun","last_name":"Abe"},{"last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","full_name":"Renkawitz, Jörg","first_name":"Jörg","orcid":"0000-0003-2856-3369"},{"last_name":"Stein","full_name":"Stein, Jens","first_name":"Jens"},{"first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt"}],"day":"18","scopus_import":"1","title":"Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells","oa_version":"Published Version"},{"department":[{"_id":"MiSi"}],"month":"11","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Gärtner, Florian R, Zerkah Ahmad, Gerhild Rosenberger, Shuxia Fan, Leo Nicolai, Benjamin Busch, Gökce Yavuz, et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>.","ista":"Gärtner FR, Ahmad Z, Rosenberger G, Fan S, Nicolai L, Busch B, Yavuz G, Luckner M, Ishikawa Ankerhold H, Hennel R, Benechet A, Lorenz M, Chandraratne S, Schubert I, Helmer S, Striednig B, Stark K, Janko M, Böttcher R, Verschoor A, Leon C, Gachet C, Gudermann T, Mederos Y Schnitzler M, Pincus Z, Iannacone M, Haas R, Wanner G, Lauber K, Sixt MK, Massberg S. 2017. Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. 171(6), 1368–1382.","mla":"Gärtner, Florian R., et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>, vol. 171, no. 6, Cell Press, 2017, pp. 1368–82, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>.","apa":"Gärtner, F. R., Ahmad, Z., Rosenberger, G., Fan, S., Nicolai, L., Busch, B., … Massberg, S. (2017). Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>","ama":"Gärtner FR, Ahmad Z, Rosenberger G, et al. Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. 2017;171(6):1368-1382. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>","short":"F.R. Gärtner, Z. Ahmad, G. Rosenberger, S. Fan, L. Nicolai, B. Busch, G. Yavuz, M. Luckner, H. Ishikawa Ankerhold, R. Hennel, A. Benechet, M. Lorenz, S. Chandraratne, I. Schubert, S. Helmer, B. Striednig, K. Stark, M. Janko, R. Böttcher, A. Verschoor, C. Leon, C. Gachet, T. Gudermann, M. Mederos Y Schnitzler, Z. Pincus, M. Iannacone, R. Haas, G. Wanner, K. Lauber, M.K. Sixt, S. Massberg, Cell Press 171 (2017) 1368–1382.","ieee":"F. R. Gärtner <i>et al.</i>, “Migrating platelets are mechano scavengers that collect and bundle bacteria,” <i>Cell Press</i>, vol. 171, no. 6. Cell Press, pp. 1368–1382, 2017."},"issue":"6","language":[{"iso":"eng"}],"date_created":"2018-12-11T11:47:15Z","volume":171,"oa_version":"None","title":"Migrating platelets are mechano scavengers that collect and bundle bacteria","scopus_import":1,"day":"30","author":[{"last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","orcid":"0000-0001-6120-3723"},{"full_name":"Ahmad, Zerkah","last_name":"Ahmad","first_name":"Zerkah"},{"full_name":"Rosenberger, Gerhild","last_name":"Rosenberger","first_name":"Gerhild"},{"first_name":"Shuxia","last_name":"Fan","full_name":"Fan, Shuxia"},{"first_name":"Leo","last_name":"Nicolai","full_name":"Nicolai, Leo"},{"full_name":"Busch, Benjamin","last_name":"Busch","first_name":"Benjamin"},{"first_name":"Gökce","last_name":"Yavuz","full_name":"Yavuz, Gökce"},{"full_name":"Luckner, Manja","last_name":"Luckner","first_name":"Manja"},{"full_name":"Ishikawa Ankerhold, Hellen","last_name":"Ishikawa Ankerhold","first_name":"Hellen"},{"first_name":"Roman","full_name":"Hennel, Roman","last_name":"Hennel"},{"full_name":"Benechet, Alexandre","last_name":"Benechet","first_name":"Alexandre"},{"first_name":"Michael","last_name":"Lorenz","full_name":"Lorenz, Michael"},{"last_name":"Chandraratne","full_name":"Chandraratne, Sue","first_name":"Sue"},{"full_name":"Schubert, Irene","last_name":"Schubert","first_name":"Irene"},{"full_name":"Helmer, Sebastian","last_name":"Helmer","first_name":"Sebastian"},{"full_name":"Striednig, Bianca","last_name":"Striednig","first_name":"Bianca"},{"full_name":"Stark, Konstantin","last_name":"Stark","first_name":"Konstantin"},{"first_name":"Marek","full_name":"Janko, Marek","last_name":"Janko"},{"last_name":"Böttcher","full_name":"Böttcher, Ralph","first_name":"Ralph"},{"full_name":"Verschoor, Admar","last_name":"Verschoor","first_name":"Admar"},{"last_name":"Leon","full_name":"Leon, Catherine","first_name":"Catherine"},{"full_name":"Gachet, Christian","last_name":"Gachet","first_name":"Christian"},{"last_name":"Gudermann","full_name":"Gudermann, Thomas","first_name":"Thomas"},{"last_name":"Mederos Y Schnitzler","full_name":"Mederos Y Schnitzler, Michael","first_name":"Michael"},{"full_name":"Pincus, Zachary","last_name":"Pincus","first_name":"Zachary"},{"first_name":"Matteo","full_name":"Iannacone, Matteo","last_name":"Iannacone"},{"first_name":"Rainer","last_name":"Haas","full_name":"Haas, Rainer"},{"last_name":"Wanner","full_name":"Wanner, Gerhard","first_name":"Gerhard"},{"full_name":"Lauber, Kirsten","last_name":"Lauber","first_name":"Kirsten"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"publication_identifier":{"issn":["00928674"]},"publication_status":"published","abstract":[{"text":"Blood platelets are critical for hemostasis and thrombosis and play diverse roles during immune responses. Despite these versatile tasks in mammalian biology, their skills on a cellular level are deemed limited, mainly consisting in rolling, adhesion, and aggregate formation. Here, we identify an unappreciated asset of platelets and show that adherent platelets use adhesion receptors to mechanically probe the adhesive substrate in their local microenvironment. When actomyosin-dependent traction forces overcome substrate resistance, platelets migrate and pile up the adhesive substrate together with any bound particulate material. They use this ability to act as cellular scavengers, scanning the vascular surface for potential invaders and collecting deposited bacteria. Microbe collection by migrating platelets boosts the activity of professional phagocytes, exacerbating inflammatory tissue injury in sepsis. This assigns platelets a central role in innate immune responses and identifies them as potential targets to dampen inflammatory tissue damage in clinical scenarios of severe systemic infection. In addition to their role in thrombosis and hemostasis, platelets can also migrate to sites of infection to help trap bacteria and clear the vascular surface.","lang":"eng"}],"intvolume":"       171","publist_id":"7243","year":"2017","date_published":"2017-11-30T00:00:00Z","ec_funded":1,"publication":"Cell Press","status":"public","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"type":"journal_article","_id":"571","date_updated":"2021-01-12T08:03:15Z","publisher":"Cell Press","doi":"10.1016/j.cell.2017.11.001","quality_controlled":"1","page":"1368 - 1382"},{"month":"03","year":"2010","publist_id":"2171","status":"public","extern":1,"publication":"Nature Methods","date_published":"2010-03-01T00:00:00Z","issue":"3","citation":{"chicago":"Riedl, Julia, Kevin Flynn, Aurelia Raducanu, Florian R Gärtner, Gisela Beck, Michael Bosl, Frank Bradke, et al. “Lifeact Mice for Studying F-Actin Dynamics.” <i>Nature Methods</i>. Nature Publishing Group, 2010. <a href=\"https://doi.org/10.1038/nmeth0310-168\">https://doi.org/10.1038/nmeth0310-168</a>.","ista":"Riedl J, Flynn K, Raducanu A, Gärtner FR, Beck G, Bosl M, Bradke F, Massberg S, Aszodi A, Sixt MK, Wedlich Söldner R. 2010. Lifeact mice for studying F-actin dynamics. Nature Methods. 7(3), 168–169.","mla":"Riedl, Julia, et al. “Lifeact Mice for Studying F-Actin Dynamics.” <i>Nature Methods</i>, vol. 7, no. 3, Nature Publishing Group, 2010, pp. 168–69, doi:<a href=\"https://doi.org/10.1038/nmeth0310-168\">10.1038/nmeth0310-168</a>.","apa":"Riedl, J., Flynn, K., Raducanu, A., Gärtner, F. R., Beck, G., Bosl, M., … Wedlich Söldner, R. (2010). Lifeact mice for studying F-actin dynamics. <i>Nature Methods</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nmeth0310-168\">https://doi.org/10.1038/nmeth0310-168</a>","ama":"Riedl J, Flynn K, Raducanu A, et al. Lifeact mice for studying F-actin dynamics. <i>Nature Methods</i>. 2010;7(3):168-169. doi:<a href=\"https://doi.org/10.1038/nmeth0310-168\">10.1038/nmeth0310-168</a>","short":"J. Riedl, K. Flynn, A. Raducanu, F.R. Gärtner, G. Beck, M. Bosl, F. Bradke, S. Massberg, A. Aszodi, M.K. Sixt, R. Wedlich Söldner, Nature Methods 7 (2010) 168–169.","ieee":"J. Riedl <i>et al.</i>, “Lifeact mice for studying F-actin dynamics,” <i>Nature Methods</i>, vol. 7, no. 3. Nature Publishing Group, pp. 168–169, 2010."},"publisher":"Nature Publishing Group","title":"Lifeact mice for studying F-actin dynamics","doi":"10.1038/nmeth0310-168","author":[{"first_name":"Julia","full_name":"Riedl, Julia","last_name":"Riedl"},{"first_name":"Kevin","last_name":"Flynn","full_name":"Flynn, Kevin C"},{"first_name":"Aurelia","last_name":"Raducanu","full_name":"Raducanu, Aurelia"},{"last_name":"Gärtner","full_name":"Florian Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723","first_name":"Florian R"},{"full_name":"Beck, Gisela","last_name":"Beck","first_name":"Gisela"},{"first_name":"Michael","last_name":"Bosl","full_name":"Bosl, Michael"},{"first_name":"Frank","full_name":"Bradke, Frank","last_name":"Bradke"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"full_name":"Aszodi, Attila","last_name":"Aszodi","first_name":"Attila"},{"first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Michael Sixt","last_name":"Sixt"},{"first_name":"Roland","full_name":"Wedlich-Söldner, Roland","last_name":"Wedlich Söldner"}],"day":"01","type":"journal_article","date_created":"2018-12-11T12:06:06Z","date_updated":"2021-01-12T07:53:28Z","_id":"3957","volume":7,"intvolume":"         7","page":"168 - 169","quality_controlled":0,"publication_status":"published"}]