---
_id: '1284'
abstract:
- lang: eng
  text: Brassinosteroids (BRs) are growth-promoting plant hormones that play a role
    in abiotic stress responses, but molecular modes that enable this activity remain
    largely unknown. Here we show that BRs participate in the regulation of freezing
    tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive
    to freezing before and after cold acclimation. The constitutive activation of
    BR signaling, in contrast, enhanced freezing resistance. Evidence is provided
    that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES)
    can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE
    ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive
    (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR
    genes are identified that are regulated in a BR- and CES-dependent manner during
    cold acclimation. A model is presented in which BRs govern different cold-responsive
    transcriptional cascades through the post-translational modification of CES and
    redundantly acting factors. This contributes to the basal resistance against freezing
    stress, but also to the further improvement of this resistance through cold acclimation.
acknowledgement: 'We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and
  bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata
  Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum
  Dürnast for plant care. This work was supported by funds from the Austrian Science
  Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4
  to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.).
  M.E. and S.J.U. were members of the TUM graduate school. '
article_processing_charge: No
author:
- first_name: Marina
  full_name: Eremina, Marina
  last_name: Eremina
- first_name: Simon
  full_name: Unterholzner, Simon
  last_name: Unterholzner
- first_name: Ajith
  full_name: Rathnayake, Ajith
  last_name: Rathnayake
- first_name: Marcos
  full_name: Castellanos, Marcos
  last_name: Castellanos
- first_name: Mamoona
  full_name: Khan-Djamei, Mamoona
  id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
  last_name: Khan-Djamei
- first_name: Karl
  full_name: Kügler, Karl
  last_name: Kügler
- first_name: Sean
  full_name: May, Sean
  last_name: May
- first_name: Klaus
  full_name: Mayer, Klaus
  last_name: Mayer
- first_name: Wilfried
  full_name: Rozhon, Wilfried
  last_name: Rozhon
- first_name: Brigitte
  full_name: Poppenberger, Brigitte
  last_name: Poppenberger
citation:
  ama: Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate
    in the control of basal and acquired freezing tolerance of plants. <i>PNAS</i>.
    2016;113(40):E5982-E5991. doi:<a href="https://doi.org/10.1073/pnas.1611477113">10.1073/pnas.1611477113</a>
  apa: Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei,
    M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the
    control of basal and acquired freezing tolerance of plants. <i>PNAS</i>. National
    Academy of Sciences. <a href="https://doi.org/10.1073/pnas.1611477113">https://doi.org/10.1073/pnas.1611477113</a>
  chicago: Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos,
    Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and
    Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and
    Acquired Freezing Tolerance of Plants.” <i>PNAS</i>. National Academy of Sciences,
    2016. <a href="https://doi.org/10.1073/pnas.1611477113">https://doi.org/10.1073/pnas.1611477113</a>.
  ieee: M. Eremina <i>et al.</i>, “Brassinosteroids participate in the control of
    basal and acquired freezing tolerance of plants,” <i>PNAS</i>, vol. 113, no. 40.
    National Academy of Sciences, pp. E5982–E5991, 2016.
  ista: Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler
    K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate
    in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40),
    E5982–E5991.
  mla: Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal
    and Acquired Freezing Tolerance of Plants.” <i>PNAS</i>, vol. 113, no. 40, National
    Academy of Sciences, 2016, pp. E5982–91, doi:<a href="https://doi.org/10.1073/pnas.1611477113">10.1073/pnas.1611477113</a>.
  short: M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei,
    K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991.
date_created: 2018-12-11T11:51:08Z
date_published: 2016-10-04T00:00:00Z
date_updated: 2022-02-18T13:41:37Z
day: '04'
doi: 10.1073/pnas.1611477113
extern: '1'
external_id:
  pmid:
  - '27489342'
intvolume: '       113'
issue: '40'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056081/
month: '10'
oa: 1
oa_version: Submitted Version
page: E5982 - E5991
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6032'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Brassinosteroids participate in the control of basal and acquired freezing
  tolerance of plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1640'
abstract:
- lang: eng
  text: Auxin and cytokinin are key endogenous regulators of plant development. Although
    cytokinin-mediated modulation of auxin distribution is a developmentally crucial
    hormonal interaction, its molecular basis is largely unknown. Here we show a direct
    regulatory link between cytokinin signalling and the auxin transport machinery
    uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that
    the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin
    perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters
    at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory
    element effectively uncouples PIN transcription from the CRF-mediated cytokinin
    regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent
    a missing cross-talk component that fine-tunes auxin transport capacity downstream
    of cytokinin signalling to control plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This work was supported by the European Research Council Starting
  Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the
  Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable
  Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.),
  by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity
  Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science
  Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders
  (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria
  through resources provided by the Bioimaging Facility (BIF), the Life Science Facility
  (LSF).
article_number: '8717'
author:
- first_name: Mária
  full_name: Šimášková, Mária
  last_name: Šimášková
- first_name: José
  full_name: O'Brien, José
  last_name: O'Brien
- first_name: Mamoona
  full_name: Khan-Djamei, Mamoona
  id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
  last_name: Khan-Djamei
- first_name: Giel
  full_name: Van Noorden, Giel
  last_name: Van Noorden
- first_name: Krisztina
  full_name: Ötvös, Krisztina
  id: 29B901B0-F248-11E8-B48F-1D18A9856A87
  last_name: Ötvös
  orcid: 0000-0002-5503-4983
- first_name: Anne
  full_name: Vieten, Anne
  last_name: Vieten
- first_name: Inge
  full_name: De Clercq, Inge
  last_name: De Clercq
- first_name: Johanna
  full_name: Van Haperen, Johanna
  last_name: Van Haperen
- first_name: Candela
  full_name: Cuesta, Candela
  id: 33A3C818-F248-11E8-B48F-1D18A9856A87
  last_name: Cuesta
  orcid: 0000-0003-1923-2410
- first_name: Klára
  full_name: Hoyerová, Klára
  last_name: Hoyerová
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
- first_name: Peter
  full_name: Marhavy, Peter
  id: 3F45B078-F248-11E8-B48F-1D18A9856A87
  last_name: Marhavy
  orcid: 0000-0001-5227-5741
- first_name: Krzysztof T
  full_name: Wabnik, Krzysztof T
  id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
  last_name: Wabnik
  orcid: 0000-0001-7263-0560
- first_name: Frank
  full_name: Van Breusegem, Frank
  last_name: Van Breusegem
- first_name: Moritz
  full_name: Nowack, Moritz
  last_name: Nowack
- first_name: Angus
  full_name: Murphy, Angus
  last_name: Murphy
- first_name: Jiřĺ
  full_name: Friml, Jiřĺ
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Dolf
  full_name: Weijers, Dolf
  last_name: Weijers
- first_name: Tom
  full_name: Beeckman, Tom
  last_name: Beeckman
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
citation:
  ama: Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate
    PIN-FORMED auxin transporters. <i>Nature Communications</i>. 2015;6. doi:<a href="https://doi.org/10.1038/ncomms9717">10.1038/ncomms9717</a>
  apa: Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten,
    A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin
    transporters. <i>Nature Communications</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncomms9717">https://doi.org/10.1038/ncomms9717</a>
  chicago: Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden,
    Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors
    Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>. Nature
    Publishing Group, 2015. <a href="https://doi.org/10.1038/ncomms9717">https://doi.org/10.1038/ncomms9717</a>.
  ieee: M. Šimášková <i>et al.</i>, “Cytokinin response factors regulate PIN-FORMED
    auxin transporters,” <i>Nature Communications</i>, vol. 6. Nature Publishing Group,
    2015.
  ista: Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De
    Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT,
    Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E.
    2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature
    Communications. 6, 8717.
  mla: Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin
    Transporters.” <i>Nature Communications</i>, vol. 6, 8717, Nature Publishing Group,
    2015, doi:<a href="https://doi.org/10.1038/ncomms9717">10.1038/ncomms9717</a>.
  short: M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten,
    I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý,
    K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T.
    Beeckman, E. Benková, Nature Communications 6 (2015).
date_created: 2018-12-11T11:53:12Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:52:11Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/ncomms9717
ec_funded: 1
file:
- access_level: open_access
  checksum: c2c84bca37401435fedf76bad0ba0579
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:18:36Z
  date_updated: 2020-07-14T12:45:08Z
  file_id: '5358'
  file_name: IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf
  file_size: 1471217
  relation: main_file
file_date_updated: 2020-07-14T12:45:08Z
has_accepted_license: '1'
intvolume: '         6'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '207362'
  name: Hormonal cross-talk in plant organogenesis
- _id: 2542D156-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 1774-B16
  name: Hormone cross-talk drives nutrient dependent plant development
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5513'
pubrep_id: '1020'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cytokinin response factors regulate PIN-FORMED auxin transporters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...