[{"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056081/","open_access":"1"}],"quality_controlled":"1","page":"E5982 - E5991","date_updated":"2022-02-18T13:41:37Z","_id":"1284","type":"journal_article","doi":"10.1073/pnas.1611477113","article_processing_charge":"No","publisher":"National Academy of Sciences","pmid":1,"acknowledgement":"We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum Dürnast for plant care. This work was supported by funds from the Austrian Science Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4 to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.). M.E. and S.J.U. were members of the TUM graduate school. ","date_published":"2016-10-04T00:00:00Z","status":"public","extern":"1","publication":"PNAS","publist_id":"6032","year":"2016","external_id":{"pmid":["27489342"]},"publication_status":"published","abstract":[{"lang":"eng","text":"Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the post-translational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation."}],"intvolume":"       113","volume":113,"date_created":"2018-12-11T11:51:08Z","author":[{"first_name":"Marina","full_name":"Eremina, Marina","last_name":"Eremina"},{"first_name":"Simon","full_name":"Unterholzner, Simon","last_name":"Unterholzner"},{"last_name":"Rathnayake","full_name":"Rathnayake, Ajith","first_name":"Ajith"},{"last_name":"Castellanos","full_name":"Castellanos, Marcos","first_name":"Marcos"},{"first_name":"Mamoona","full_name":"Khan-Djamei, Mamoona","id":"391B5BBC-F248-11E8-B48F-1D18A9856A87","last_name":"Khan-Djamei"},{"first_name":"Karl","last_name":"Kügler","full_name":"Kügler, Karl"},{"last_name":"May","full_name":"May, Sean","first_name":"Sean"},{"first_name":"Klaus","full_name":"Mayer, Klaus","last_name":"Mayer"},{"first_name":"Wilfried","last_name":"Rozhon","full_name":"Rozhon, Wilfried"},{"last_name":"Poppenberger","full_name":"Poppenberger, Brigitte","first_name":"Brigitte"}],"scopus_import":"1","day":"04","oa_version":"Submitted Version","title":"Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants","issue":"40","citation":{"ama":"Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. <i>PNAS</i>. 2016;113(40):E5982-E5991. doi:<a href=\"https://doi.org/10.1073/pnas.1611477113\">10.1073/pnas.1611477113</a>","ieee":"M. Eremina <i>et al.</i>, “Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants,” <i>PNAS</i>, vol. 113, no. 40. National Academy of Sciences, pp. E5982–E5991, 2016.","short":"M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei, K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991.","chicago":"Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos, Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1611477113\">https://doi.org/10.1073/pnas.1611477113</a>.","ista":"Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40), E5982–E5991.","apa":"Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei, M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1611477113\">https://doi.org/10.1073/pnas.1611477113</a>","mla":"Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” <i>PNAS</i>, vol. 113, no. 40, National Academy of Sciences, 2016, pp. E5982–91, doi:<a href=\"https://doi.org/10.1073/pnas.1611477113\">10.1073/pnas.1611477113</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"language":[{"iso":"eng"}],"month":"10"},{"citation":{"mla":"Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>, vol. 6, 8717, Nature Publishing Group, 2015, doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>.","apa":"Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten, A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>","chicago":"Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden, Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>.","ista":"Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT, Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E. 2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 6, 8717.","short":"M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten, I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý, K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T. Beeckman, E. Benková, Nature Communications 6 (2015).","ieee":"M. Šimášková <i>et al.</i>, “Cytokinin response factors regulate PIN-FORMED auxin transporters,” <i>Nature Communications</i>, vol. 6. Nature Publishing Group, 2015.","ama":"Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. 2015;6. doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"pubrep_id":"1020","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"file":[{"checksum":"c2c84bca37401435fedf76bad0ba0579","relation":"main_file","content_type":"application/pdf","access_level":"open_access","file_name":"IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf","file_id":"5358","date_updated":"2020-07-14T12:45:08Z","creator":"system","file_size":1471217,"date_created":"2018-12-12T10:18:36Z"}],"article_number":"8717","month":"01","publication_status":"published","file_date_updated":"2020-07-14T12:45:08Z","has_accepted_license":"1","intvolume":"         6","abstract":[{"text":"Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":6,"date_created":"2018-12-11T11:53:12Z","author":[{"first_name":"Mária","full_name":"Šimášková, Mária","last_name":"Šimášková"},{"first_name":"José","full_name":"O'Brien, José","last_name":"O'Brien"},{"first_name":"Mamoona","last_name":"Khan-Djamei","id":"391B5BBC-F248-11E8-B48F-1D18A9856A87","full_name":"Khan-Djamei, Mamoona"},{"last_name":"Van Noorden","full_name":"Van Noorden, Giel","first_name":"Giel"},{"first_name":"Krisztina","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","last_name":"Ötvös"},{"first_name":"Anne","last_name":"Vieten","full_name":"Vieten, Anne"},{"last_name":"De Clercq","full_name":"De Clercq, Inge","first_name":"Inge"},{"first_name":"Johanna","full_name":"Van Haperen, Johanna","last_name":"Van Haperen"},{"first_name":"Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","full_name":"Cuesta, Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Klára","full_name":"Hoyerová, Klára","last_name":"Hoyerová"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"orcid":"0000-0001-5227-5741","first_name":"Peter","last_name":"Marhavy","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavy, Peter"},{"first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","last_name":"Wabnik","full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Frank","last_name":"Van Breusegem","full_name":"Van Breusegem, Frank"},{"full_name":"Nowack, Moritz","last_name":"Nowack","first_name":"Moritz"},{"first_name":"Angus","last_name":"Murphy","full_name":"Murphy, Angus"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiřĺ","orcid":"0000-0002-8302-7596","first_name":"Jiřĺ"},{"full_name":"Weijers, Dolf","last_name":"Weijers","first_name":"Dolf"},{"first_name":"Tom","last_name":"Beeckman","full_name":"Beeckman, Tom"},{"last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"}],"day":"01","scopus_import":1,"oa_version":"Submitted Version","title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","ec_funded":1,"acknowledgement":"This work was supported by the European Research Council Starting Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.), by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF).","date_published":"2015-01-01T00:00:00Z","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16"}],"publication":"Nature Communications","status":"public","publist_id":"5513","year":"2015","quality_controlled":"1","ddc":["580"],"date_updated":"2021-01-12T06:52:11Z","_id":"1640","type":"journal_article","doi":"10.1038/ncomms9717","publisher":"Nature Publishing Group"}]