---
_id: '8582'
abstract:
- lang: eng
  text: "Cell and tissue polarization is fundamental for plant growth and morphogenesis.
    The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial
    for their function in directional auxin transport. The clustering of PIN polar
    cargoes within the plasma membrane has been proposed to be important for the maintenance
    of their polar distribution. However, the more detailed features of PIN clusters
    and the cellular requirements of cargo clustering remain unclear.\r\nHere, we
    characterized PIN clusters in detail by means of multiple advanced microscopy
    and quantification methods, such as 3D quantitative imaging or freeze‐fracture
    replica labeling. The size and aggregation types of PIN clusters were determined
    by electron microscopy at the nanometer level at different polar domains and at
    different developmental stages, revealing a strong preference for clustering at
    the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters
    depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall
    components as well as connections between the cell wall and the plasma membrane.\r\nThis
    study identifies the role of different cellular processes and structures in polar
    cargo clustering and provides initial mechanistic insight into the maintenance
    of polarity in plants and other systems."
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg)
  for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1
  mutant and the complementation lines, and Dr Patricia C. Zambryski (University of
  California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria)
  for assistance with imaging, group members for discussions, Martine De Cock for
  help in preparing the manuscript and Nataliia Gnyliukh for critical reading and
  revision of the manuscript. This project received funding from the European Research
  Council (ERC) under the European Union's Horizon 2020 research and innovation program
  (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y
  Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People
  Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme
  (FP7/2007‐2013) under REA grant agreement no. 291734.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hongjiang
  full_name: Li, Hongjiang
  id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0001-5039-9660
- first_name: Daniel
  full_name: von Wangenheim, Daniel
  id: 49E91952-F248-11E8-B48F-1D18A9856A87
  last_name: von Wangenheim
  orcid: 0000-0002-6862-1247
- first_name: Xixi
  full_name: Zhang, Xixi
  id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
  last_name: Zhang
  orcid: 0000-0001-7048-4627
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Nasser
  full_name: Darwish-Miranda, Nasser
  id: 39CD9926-F248-11E8-B48F-1D18A9856A87
  last_name: Darwish-Miranda
  orcid: 0000-0002-8821-8236
- first_name: Satoshi
  full_name: Naramoto, Satoshi
  last_name: Naramoto
- first_name: Krzysztof T
  full_name: Wabnik, Krzysztof T
  id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
  last_name: Wabnik
  orcid: 0000-0001-7263-0560
- first_name: Riet
  full_name: de Rycke, Riet
  last_name: de Rycke
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Daniel J
  full_name: Gütl, Daniel J
  id: 381929CE-F248-11E8-B48F-1D18A9856A87
  last_name: Gütl
- first_name: Ricardo
  full_name: Tejos, Ricardo
  last_name: Tejos
- first_name: Peter
  full_name: Grones, Peter
  id: 399876EC-F248-11E8-B48F-1D18A9856A87
  last_name: Grones
- first_name: Meiyu
  full_name: Ke, Meiyu
  last_name: Ke
- first_name: Xu
  full_name: Chen, Xu
  id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
  last_name: Chen
- first_name: Jan
  full_name: Dettmer, Jan
  last_name: Dettmer
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar
    cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369.
    doi:<a href="https://doi.org/10.1111/nph.16887">10.1111/nph.16887</a>
  apa: Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto,
    S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in
    Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href="https://doi.org/10.1111/nph.16887">https://doi.org/10.1111/nph.16887</a>
  chicago: Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda,
    Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar
    Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021.
    <a href="https://doi.org/10.1111/nph.16887">https://doi.org/10.1111/nph.16887</a>.
  ieee: H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering
    in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp.
    351–369, 2021.
  ista: Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik
    KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer
    J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis
    thaliana. New Phytologist. 229(1), 351–369.
  mla: Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering
    in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021,
    pp. 351–69, doi:<a href="https://doi.org/10.1111/nph.16887">10.1111/nph.16887</a>.
  short: H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto,
    K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke,
    X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.
date_created: 2020-09-28T08:59:28Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-08-04T11:01:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
- _id: EvBe
doi: 10.1111/nph.16887
ec_funded: 1
external_id:
  isi:
  - '000570187900001'
file:
- access_level: open_access
  checksum: b45621607b4cab97eeb1605ab58e896e
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-04T09:44:17Z
  date_updated: 2021-02-04T09:44:17Z
  file_id: '9084'
  file_name: 2021_NewPhytologist_Li.pdf
  file_size: 4061962
  relation: main_file
  success: 1
file_date_updated: 2021-02-04T09:44:17Z
has_accepted_license: '1'
intvolume: '       229'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 351-369
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
  eissn:
  - '14698137'
  issn:
  - 0028646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 229
year: '2021'
...
---
_id: '6087'
abstract:
- lang: eng
  text: Cell fate specification by lateral inhibition typically involves contact signaling
    through the Delta-Notch signaling pathway. However, whether this is the only signaling
    mode mediating lateral inhibition remains unclear. Here we show that in zebrafish
    oogenesis, a group of cells within the granulosa cell layer at the oocyte animal
    pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei.
    One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly
    high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically
    compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly,
    relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear
    TAZ accumulation in neighboring cells, eventually leading to MPC re-specification
    from these cells. Conversely, MPC specification is defective in taz−/− follicles.
    These findings uncover a novel mode of lateral inhibition in cell fate specification
    based on mechanical signals controlling TAZ activity.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins,
  and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra
  Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance
  and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish
  facilities of IST Austria for continuous support. This work was supported by an
  ERC advanced grant ( MECSPEC to C.-P.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Daniel J
  full_name: Gütl, Daniel J
  id: 381929CE-F248-11E8-B48F-1D18A9856A87
  last_name: Gütl
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification
    mediated by mechanical signals modulating TAZ activity. <i>Cell</i>. 2019;176(6):1379-1392.e14.
    doi:<a href="https://doi.org/10.1016/j.cell.2019.01.019">10.1016/j.cell.2019.01.019</a>
  apa: Xia, P., Gütl, D. J., Zheden, V., &#38; Heisenberg, C.-P. J. (2019). Lateral
    inhibition in cell specification mediated by mechanical signals modulating TAZ
    activity. <i>Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.cell.2019.01.019">https://doi.org/10.1016/j.cell.2019.01.019</a>
  chicago: Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg.
    “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating
    TAZ Activity.” <i>Cell</i>. Elsevier, 2019. <a href="https://doi.org/10.1016/j.cell.2019.01.019">https://doi.org/10.1016/j.cell.2019.01.019</a>.
  ieee: P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition
    in cell specification mediated by mechanical signals modulating TAZ activity,”
    <i>Cell</i>, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019.
  ista: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell
    specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6),
    1379–1392.e14.
  mla: Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical
    Signals Modulating TAZ Activity.” <i>Cell</i>, vol. 176, no. 6, Elsevier, 2019,
    p. 1379–1392.e14, doi:<a href="https://doi.org/10.1016/j.cell.2019.01.019">10.1016/j.cell.2019.01.019</a>.
  short: P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.
date_created: 2019-03-10T22:59:19Z
date_published: 2019-03-07T00:00:00Z
date_updated: 2023-08-25T08:02:23Z
day: '07'
department:
- _id: CaHe
- _id: EM-Fac
doi: 10.1016/j.cell.2019.01.019
ec_funded: 1
external_id:
  isi:
  - '000460509600013'
  pmid:
  - '30773315'
intvolume: '       176'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.cell.2019.01.019
month: '03'
oa: 1
oa_version: Published Version
page: 1379-1392.e14
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/
scopus_import: '1'
status: public
title: Lateral inhibition in cell specification mediated by mechanical signals modulating
  TAZ activity
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 176
year: '2019'
...