---
_id: '3289'
abstract:
- lang: eng
  text: "Viral manipulation of transduction pathways associated with key cellular
    functions such as survival, response to microbial infection, and cytoskeleton
    reorganization can provide the supportive milieu for a productive infection. Here,
    we demonstrate that vaccinia virus (VACV) infection leads to activation of the
    stress-activated protein kinase (SAPK)/extracellular signal-regulated kinase (ERK)
    4/7 (MKK4/7)-c-Jun N-terminal protein kinase 1/2 (JNK1/2) pathway; further, the
    stimulation of this pathway requires postpenetration, prereplicative events in
    the viral replication cycle. Although the formation of intracellular mature virus
    (IMV) was not affected in MKK4/7- or JNK1/2-knockout (KO) cells, we did note an
    accentuated deregulation of microtubule and actin network organization in infected
    JNK1/2-KO cells. This was followed by deregulated viral trafficking to the periphery
    and enhanced enveloped particle release. Furthermore, VACV infection induced alterations
    in the cell contractility and morphology, and cell migration was reduced in the
    JNK-KO cells. In addition, phosphorylation of proteins implicated with early cell
    contractility and cell migration, such as microtubule-associated protein 1B and
    paxillin, respectively, was not detected in the VACV-infected KO cells. In sum,
    our findings uncover a regulatory role played by the MKK4/7-JNK1/2 pathway in
    cytoskeleton reorganization during VACV infection.\r\n"
acknowledgement: "This work was supported by grants from Fundação de Amparo a Pesquisa
  do Estado de Minas Gerais (FAPEMIG), the Brazilian Federal Agency for Support and
  Evaluation of Graduate Education (CAPES), and the National Council for Scientific
  and Technological Development (CNPq). A.C.T.C.P., B.S.A.F.B., F.G.G.L., and J.A.P.S.-M.
  were recipients of predoctoral fellowships from CNPq. C.A.B., E.G.K., T.S.-P., P.F.P.P.,
  and P.C.P.F. are recipients of research fellowships from CNPq. \r\n\r\n\r\nWe are
  grateful to Angela S. Lopes, Ilda M. V. Gama, João R. dos Santos, and Andreza A.
  Carvalho for their secretarial/technical assistance and to Fernanda Gambogi for
  help with immunofluorescence microscopy. We also thank M. C. Sogayar (Department
  of Biochemistry, University of São Paulo, São Paulo, Brazil), who kindly provided
  us with the A31 cell line, and R. Davis (Howard Hughes Medical Institute, University
  of Massachusetts Medical School, Worcester, MA) for the WT and JNK1/2-, MKK4-, MKK7-,
  and MKK4/7-KO cells. VACV WR was from C. Jungwirth (Universität Würzburg, Würzburg,
  Germany). The recombinant VACV vF13L-GFP and the rabbit polyclonal antibodies against
  viral proteins, B5R, D8L, L1R, and A36R, were from B. Moss (NIAID, Bethesda, MD).
  The pcDNA3-Myc-JNK2-MKK7 WT plasmid was from Eugen Kerkhoff (Universität Würzburg,
  Würzburg, Germany). We also thank Flávio G. da Fonseca (UFMG, Belo Horizonte, MG,
  Brazil) and Kathleen A. Boyle (Medical College of Wisconsin, Milwaukee, WI) for
  critically reading the manuscript."
author:
- first_name: Anna
  full_name: Pereira, Anna
  last_name: Pereira
- first_name: Flávia
  full_name: Leite, Flávia
  id: 36705F98-F248-11E8-B48F-1D18A9856A87
  last_name: Leite
- first_name: Bruno
  full_name: Brasil, Bruno
  last_name: Brasil
- first_name: Jamaria
  full_name: Soares Martins, Jamaria
  last_name: Soares Martins
- first_name: Alice
  full_name: Torres, Alice
  last_name: Torres
- first_name: Paulo
  full_name: Pimenta, Paulo
  last_name: Pimenta
- first_name: Thais
  full_name: Souto Padrón, Thais
  last_name: Souto Padrón
- first_name: Paula
  full_name: Tranktman, Paula
  last_name: Tranktman
- first_name: Paulo
  full_name: Ferreira, Paulo
  last_name: Ferreira
- first_name: Erna
  full_name: Kroon, Erna
  last_name: Kroon
- first_name: Cláudio
  full_name: Bonjardim, Cláudio
  last_name: Bonjardim
citation:
  ama: Pereira A, Leite F, Brasil B, et al. A vaccinia virus-driven interplay between
    the MKK4/7-JNK1/2 pathway and cytoskeleton reorganization. <i>Journal of Virology</i>.
    2012;86(1):172-184. doi:<a href="https://doi.org/10.1128/JVI.05638-11">10.1128/JVI.05638-11</a>
  apa: Pereira, A., Leite, F., Brasil, B., Soares Martins, J., Torres, A., Pimenta,
    P., … Bonjardim, C. (2012). A vaccinia virus-driven interplay between the MKK4/7-JNK1/2
    pathway and cytoskeleton reorganization. <i>Journal of Virology</i>. ASM. <a href="https://doi.org/10.1128/JVI.05638-11">https://doi.org/10.1128/JVI.05638-11</a>
  chicago: Pereira, Anna, Flávia Leite, Bruno Brasil, Jamaria Soares Martins, Alice
    Torres, Paulo Pimenta, Thais Souto Padrón, et al. “A Vaccinia Virus-Driven Interplay
    between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization.” <i>Journal
    of Virology</i>. ASM, 2012. <a href="https://doi.org/10.1128/JVI.05638-11">https://doi.org/10.1128/JVI.05638-11</a>.
  ieee: A. Pereira <i>et al.</i>, “A vaccinia virus-driven interplay between the MKK4/7-JNK1/2
    pathway and cytoskeleton reorganization,” <i>Journal of Virology</i>, vol. 86,
    no. 1. ASM, pp. 172–184, 2012.
  ista: Pereira A, Leite F, Brasil B, Soares Martins J, Torres A, Pimenta P, Souto
    Padrón T, Tranktman P, Ferreira P, Kroon E, Bonjardim C. 2012. A vaccinia virus-driven
    interplay between the MKK4/7-JNK1/2 pathway and cytoskeleton reorganization. Journal
    of Virology. 86(1), 172–184.
  mla: Pereira, Anna, et al. “A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2
    Pathway and Cytoskeleton Reorganization.” <i>Journal of Virology</i>, vol. 86,
    no. 1, ASM, 2012, pp. 172–84, doi:<a href="https://doi.org/10.1128/JVI.05638-11">10.1128/JVI.05638-11</a>.
  short: A. Pereira, F. Leite, B. Brasil, J. Soares Martins, A. Torres, P. Pimenta,
    T. Souto Padrón, P. Tranktman, P. Ferreira, E. Kroon, C. Bonjardim, Journal of
    Virology 86 (2012) 172–184.
date_created: 2018-12-11T12:02:29Z
date_published: 2012-01-01T00:00:00Z
date_updated: 2021-01-12T07:42:25Z
day: '01'
department:
- _id: JoBo
doi: 10.1128/JVI.05638-11
external_id:
  pmid:
  - '22031940'
intvolume: '        86'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255887/
month: '01'
oa: 1
oa_version: Submitted Version
page: 172 - 184
pmid: 1
publication: Journal of Virology
publication_status: published
publisher: ASM
publist_id: '3356'
quality_controlled: '1'
scopus_import: 1
status: public
title: A vaccinia virus-driven interplay between the MKK4/7-JNK1/2 pathway and cytoskeleton
  reorganization
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 86
year: '2012'
...