---
_id: '14591'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth
    and development by controlling plasma membrane protein composition and cargo uptake.
    CME relies on the precise recruitment of regulators for vesicle maturation and
    release. Homologues of components of mammalian vesicle scission are strong candidates
    to be part of the scissin machinery in plants, but the precise roles of these
    proteins in this process is not fully understood. Here, we characterised the roles
    of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein
    2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin,
    in the CME by combining high-resolution imaging of endocytic events in vivo and
    characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive
    similarly late during CME and physically interact, genetic analysis of the Dsh3p1,2,3
    triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants
    suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis.
    These observations imply that despite the presence of many well-conserved endocytic
    components, plants have acquired a distinct mechanism for CME. One Sentence Summary
    In contrast to predictions based on mammalian systems, plant Dynamin-related proteins
    2 are recruited to the site of Clathrin-mediated endocytosis independently of
    BAR-SH3 proteins.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
article_processing_charge: No
author:
- first_name: Nataliia
  full_name: Gnyliukh, Nataliia
  id: 390C1120-F248-11E8-B48F-1D18A9856A87
  last_name: Gnyliukh
  orcid: 0000-0002-2198-0509
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Marie-Kristin
  full_name: Nagel, Marie-Kristin
  last_name: Nagel
- first_name: Aline
  full_name: Monzer, Aline
  id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
  last_name: Monzer
- first_name: Annamaria
  full_name: Hlavata, Annamaria
  id: 36062FEC-F248-11E8-B48F-1D18A9856A87
  last_name: Hlavata
- first_name: Erika
  full_name: Isono, Erika
  last_name: Isono
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Gnyliukh N, Johnson AJ, Nagel M-K, et al. Role of dynamin-related proteins
    2 and SH3P2 in clathrin-mediated endocytosis in plants. <i>bioRxiv</i>. doi:<a
    href="https://doi.org/10.1101/2023.10.09.561523">10.1101/2023.10.09.561523</a>
  apa: Gnyliukh, N., Johnson, A. J., Nagel, M.-K., Monzer, A., Hlavata, A., Isono,
    E., … Friml, J. (n.d.). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated
    endocytosis in plants. <i>bioRxiv</i>. <a href="https://doi.org/10.1101/2023.10.09.561523">https://doi.org/10.1101/2023.10.09.561523</a>
  chicago: Gnyliukh, Nataliia, Alexander J Johnson, Marie-Kristin Nagel, Aline Monzer,
    Annamaria Hlavata, Erika Isono, Martin Loose, and Jiří Friml. “Role of Dynamin-Related
    Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Plants.” <i>BioRxiv</i>,
    n.d. <a href="https://doi.org/10.1101/2023.10.09.561523">https://doi.org/10.1101/2023.10.09.561523</a>.
  ieee: N. Gnyliukh <i>et al.</i>, “Role of dynamin-related proteins 2 and SH3P2 in
    clathrin-mediated endocytosis in plants,” <i>bioRxiv</i>. .
  ista: Gnyliukh N, Johnson AJ, Nagel M-K, Monzer A, Hlavata A, Isono E, Loose M,
    Friml J. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis
    in plants. bioRxiv, <a href="https://doi.org/10.1101/2023.10.09.561523">10.1101/2023.10.09.561523</a>.
  mla: Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in
    Clathrin-Mediated Endocytosis in Plants.” <i>BioRxiv</i>, doi:<a href="https://doi.org/10.1101/2023.10.09.561523">10.1101/2023.10.09.561523</a>.
  short: N. Gnyliukh, A.J. Johnson, M.-K. Nagel, A. Monzer, A. Hlavata, E. Isono,
    M. Loose, J. Friml, BioRxiv (n.d.).
date_created: 2023-11-22T10:17:49Z
date_published: 2023-10-10T00:00:00Z
date_updated: 2023-12-01T13:51:06Z
day: '10'
department:
- _id: JiFr
- _id: MaLo
- _id: CaBe
doi: 10.1101/2023.10.09.561523
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/2023.10.09.561523v2
month: '10'
oa: 1
oa_version: Preprint
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
publication: bioRxiv
publication_status: submitted
related_material:
  record:
  - id: '14510'
    relation: dissertation_contains
    status: public
status: public
title: Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis
  in plants
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...