---
_id: '12291'
abstract:
- lang: eng
text: The phytohormone auxin triggers transcriptional reprogramming through a well-characterized
perception machinery in the nucleus. By contrast, mechanisms that underlie fast
effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation
of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding
protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4.
Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds
auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its
plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required
for the auxin-induced ultrafast global phospho-response and for downstream processes
that include the activation of H+-ATPase and accelerated cytoplasmic streaming.
abp1 and tmk mutants cannot establish auxin-transporting channels and show defective
auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that
lacks the capacity to bind auxin is unable to complement these defects in abp1
mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface
signalling, which mediates the global phospho-response and auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We acknowledge K. Kubiasová for excellent technical assistance, J.
Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production
and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI;
and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the
Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM
(MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F.
is grateful to R. Napier for many insightful suggestions and support. We thank all
past and present members of the Friml group for their support and for other contributions
to this effort to clarify the controversial role of ABP1 over the past seven years.
The project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.);
the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001
to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science
and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053
to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and
20H05910).
article_processing_charge: No
article_type: original
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Branka D.
full_name: Živanović, Branka D.
last_name: Živanović
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Peter
full_name: Grones, Peter
last_name: Grones
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Nikola
full_name: Rýdza, Nikola
last_name: Rýdza
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
citation:
ama: Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation
and auxin canalization. Nature. 2022;609(7927):575-581. doi:10.1038/s41586-022-05187-x
apa: Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A.,
… Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and
auxin canalization. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05187-x
chicago: Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa
Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception
for Global Phosphorylation and Auxin Canalization.” Nature. Springer Nature,
2022. https://doi.org/10.1038/s41586-022-05187-x.
ieee: J. Friml et al., “ABP1–TMK auxin perception for global phosphorylation
and auxin canalization,” Nature, vol. 609, no. 7927. Springer Nature, pp.
575–581, 2022.
ista: Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey
L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones
P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K,
Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception
for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.
mla: Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and
Auxin Canalization.” Nature, vol. 609, no. 7927, Springer Nature, 2022,
pp. 575–81, doi:10.1038/s41586-022-05187-x.
short: J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez
Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini,
P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza,
K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature
609 (2022) 575–581.
date_created: 2023-01-16T10:04:48Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-11-07T08:16:09Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: EvBe
- _id: EM-Fac
doi: 10.1038/s41586-022-05187-x
ec_funded: 1
external_id:
isi:
- '000851357500002'
pmid:
- '36071161'
file:
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checksum: a6055c606aefb900bf62ae3e7d15f921
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creator: amally
date_created: 2023-11-02T17:12:37Z
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month: '09'
oa: 1
oa_version: Submitted Version
page: 575-581
pmid: 1
project:
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: ABP1–TMK auxin perception for global phosphorylation and auxin canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 609
year: '2022'
...
---
_id: '10411'
abstract:
- lang: eng
text: The phytohormone auxin is the major growth regulator governing tropic responses
including gravitropism. Auxin build-up at the lower side of stimulated shoots
promotes cell expansion, whereas in roots it inhibits growth, leading to upward
shoot bending and downward root bending, respectively. Yet it remains an enigma
how the same signal can trigger such opposite cellular responses. In this review,
we discuss several recent unexpected insights into the mechanisms underlying auxin
regulation of growth, challenging several existing models. We focus on the divergent
mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical
Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling
pathways. From this emerges a more comprehensive, updated picture how auxin regulates
growth.
acknowledgement: The authors thank Alexandra Mally for editing the text. This work
was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the
DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were
created with BioRender.com.
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 2022;27(5):440-449. doi:10.1016/j.tplants.2021.11.006'
apa: 'Li, L., Gallei, M. C., & Friml, J. (2022). Bending to auxin: Fast acid
growth for tropisms. Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2021.11.006'
chicago: 'Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast
Acid Growth for Tropisms.” Trends in Plant Science. Cell Press, 2022. https://doi.org/10.1016/j.tplants.2021.11.006.'
ieee: 'L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for
tropisms,” Trends in Plant Science, vol. 27, no. 5. Cell Press, pp. 440–449,
2022.'
ista: 'Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 27(5), 440–449.'
mla: 'Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” Trends
in Plant Science, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:10.1016/j.tplants.2021.11.006.'
short: L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449.
date_created: 2021-12-05T23:01:43Z
date_published: 2022-05-01T00:00:00Z
date_updated: 2024-10-29T10:12:33Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2021.11.006
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call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Trends in Plant Science
publication_identifier:
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- 1360-1385
publication_status: published
publisher: Cell Press
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record:
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scopus_import: '1'
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title: 'Bending to auxin: Fast acid growth for tropisms'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2022'
...
---
_id: '11626'
abstract:
- lang: eng
text: Plant growth and development is well known to be both, flexible and dynamic.
The high capacity for post-embryonic organ formation and tissue regeneration requires
tightly regulated intercellular communication and coordinated tissue polarization.
One of the most important drivers for patterning and polarity in plant development
is the phytohormone auxin. Auxin has the unique characteristic to establish polarized
channels for its own active directional cell to cell transport. This fascinating
phenomenon is called auxin canalization. Those auxin transport channels are characterized
by the expression and polar, subcellular localization of PIN auxin efflux carriers.
PIN proteins have the ability to dynamically change their localization and auxin
itself can affect this by interfering with trafficking. Most of the underlying
molecular mechanisms of canalization still remain enigmatic. What is known so
far is that canonical auxin signaling is indispensable but also other non-canonical
signaling components are thought to play a role. In order to shed light into the
mysteries auf auxin canalization this study revisits the branches of auxin signaling
in detail. Further a new auxin analogue, PISA, is developed which triggers auxin-like
responses but does not directly activate canonical transcriptional auxin signaling.
We revisit the direct auxin effect on PIN trafficking where we found that, contradictory
to previous observations, auxin is very specifically promoting endocytosis of
PIN2 but has no overall effect on endocytosis. Further, we evaluate which cellular
processes related to PIN subcellular dynamics are involved in the establishment
of auxin conducting channels and the formation of vascular tissue. We are re-evaluating
the function of AUXIN BINDING PROTEIN 1 (ABP1) and provide a comprehensive picture
about its developmental phneotypes and involvement in auxin signaling and canalization.
Lastly, we are focusing on the crosstalk between the hormone strigolactone (SL)
and auxin and found that SL is interfering with essentially all processes involved
in auxin canalization in a non-transcriptional manner. Lastly we identify a new
way of SL perception and signaling which is emanating from mitochondria, is independent
of canonical SL signaling and is modulating primary root growth.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
citation:
ama: Gallei MC. Auxin and strigolactone non-canonical signaling regulating development
in Arabidopsis thaliana. 2022. doi:10.15479/at:ista:11626
apa: Gallei, M. C. (2022). Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana. Institute of Science and Technology Austria.
https://doi.org/10.15479/at:ista:11626
chicago: Gallei, Michelle C. “Auxin and Strigolactone Non-Canonical Signaling Regulating
Development in Arabidopsis Thaliana.” Institute of Science and Technology Austria,
2022. https://doi.org/10.15479/at:ista:11626.
ieee: M. C. Gallei, “Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana,” Institute of Science and Technology Austria,
2022.
ista: Gallei MC. 2022. Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana. Institute of Science and Technology Austria.
mla: Gallei, Michelle C. Auxin and Strigolactone Non-Canonical Signaling Regulating
Development in Arabidopsis Thaliana. Institute of Science and Technology Austria,
2022, doi:10.15479/at:ista:11626.
short: M.C. Gallei, Auxin and Strigolactone Non-Canonical Signaling Regulating Development
in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2022.
date_created: 2022-07-20T11:21:53Z
date_published: 2022-07-20T00:00:00Z
date_updated: 2024-10-29T10:22:45Z
day: '20'
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degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
doi: 10.15479/at:ista:11626
ec_funded: 1
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month: '07'
oa: 1
oa_version: Published Version
page: '248'
project:
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication_identifier:
isbn:
- 978-3-99078-019-0
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '9287'
relation: part_of_dissertation
status: public
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relation: part_of_dissertation
status: public
- id: '7465'
relation: part_of_dissertation
status: public
- id: '8138'
relation: part_of_dissertation
status: public
- id: '6260'
relation: part_of_dissertation
status: public
- id: '8931'
relation: part_of_dissertation
status: public
- id: '10411'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Eilon
full_name: Shani, Eilon
last_name: Shani
title: Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis
thaliana
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2022'
...
---
_id: '8931'
abstract:
- lang: eng
text: "Auxin is a major plant growth regulator, but current models on auxin perception
and signaling cannot explain the whole plethora of auxin effects, in particular
those associated with rapid responses. A possible candidate for a component of
additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1),
whose function in planta remains unclear.\r\nHere we combined expression analysis
with gain- and loss-of-function approaches to analyze the role of ABP1 in plant
development. ABP1 shows a broad expression largely overlapping with, but not regulated
by, transcriptional auxin response activity. Furthermore, ABP1 activity is not
essential for the transcriptional auxin signaling. Genetic in planta analysis
revealed that abp1 loss-of-function mutants show largely normal development with
minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show
a broad range of growth and developmental defects, including root and hypocotyl
growth and bending, lateral root and leaf development, bolting, as well as response
to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired
auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular
aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically
unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function
mutants by a functional redundancy."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We would like to acknowledge Bioimaging and Life Science Facilities
at IST Austria for continuous support and also the Plant Sciences Core Facility
of CEITEC Masaryk University for their support with obtaining a part of the scientific
data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct
design. This project has received funding from the European Research Council (ERC)
under the European Union’s Horizon 2020 research and innovation program [grant agreement
no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship
of the Austrian Academy of Sciences to L.L.; the European Structural and Investment
Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“
[CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by
the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738]
to M. Č.
article_number: '110750'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Géraldine
full_name: Brunoud, Géraldine
last_name: Brunoud
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Zlata
full_name: Pavlovicova, Zlata
last_name: Pavlovicova
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
- first_name: Teva
full_name: Vernoux, Teva
last_name: Vernoux
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding
protein 1 in Arabidopsis thaliana. Plant Science. 2021;303. doi:10.1016/j.plantsci.2020.110750
apa: Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M.,
… Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis
thaliana. Plant Science. Elsevier. https://doi.org/10.1016/j.plantsci.2020.110750
chicago: Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud,
Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding
Protein 1 in Arabidopsis Thaliana.” Plant Science. Elsevier, 2021. https://doi.org/10.1016/j.plantsci.2020.110750.
ieee: Z. Gelová et al., “Developmental roles of auxin binding protein 1 in
Arabidopsis thaliana,” Plant Science, vol. 303. Elsevier, 2021.
ista: Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko
J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka
M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles
of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.
mla: Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis
Thaliana.” Plant Science, vol. 303, 110750, Elsevier, 2021, doi:10.1016/j.plantsci.2020.110750.
short: Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L.
Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild,
M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml,
Plant Science 303 (2021).
date_created: 2020-12-09T14:48:28Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-10-29T10:22:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.1016/j.plantsci.2020.110750
ec_funded: 1
external_id:
isi:
- '000614154500001'
pmid:
- '33487339'
file:
- access_level: open_access
checksum: a7f2562bdca62d67dfa88e271b62a629
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T07:49:25Z
date_updated: 2021-02-04T07:49:25Z
file_id: '9083'
file_name: 2021_PlantScience_Gelova.pdf
file_size: 12563728
relation: main_file
success: 1
file_date_updated: 2021-02-04T07:49:25Z
has_accepted_license: '1'
intvolume: ' 303'
isi: 1
keyword:
- Agronomy and Crop Science
- Plant Science
- Genetics
- General Medicine
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Plant Science
publication_identifier:
issn:
- 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Developmental roles of auxin binding protein 1 in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 303
year: '2021'
...
---
_id: '9287'
abstract:
- lang: eng
text: "The phytohormone auxin and its directional transport through tissues are
intensively studied. However, a mechanistic understanding of auxin-mediated feedback
on endocytosis and polar distribution of PIN auxin transporters remains limited
due to contradictory observations and interpretations. Here, we used state-of-the-art
methods to reexamine the\r\nauxin effects on PIN endocytic trafficking. We used
high auxin concentrations or longer treatments versus lower concentrations and
shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish
between specific and nonspecific effects. Longer treatments of both auxins interfere
with Brefeldin A-mediated intracellular PIN2 accumulation and also with general
aggregation of endomembrane compartments. NAA treatment decreased the internalization
of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the
number, distribution, and compartment identity of the early endosome/trans-Golgi
network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations
unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the
endomembrane system, we opted for alternative approaches visualizing the endocytic
events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence
(TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the
incidence and dynamics of clathrin foci, implying that these treatments do not
affect the overall endocytosis rate. However, both NAA and IAA at low concentrations
rapidly and specifically promoted endocytosis of photo-converted PIN2 from the
PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis,
thus contributing to its\r\npolarity maintenance and furthermore illustrate that
high auxin levels have nonspecific effects on trafficking and endomembrane compartments. "
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: 'We thank Ivan Kulik for developing the Chip’n’Dale apparatus with
Lanxin Li; the IST machine shop and the Bioimaging facility for their excellent
support; Matouš Glanc and Matyáš Fendrych for their valuable discussions and help;
Barbara Casillas-Perez for her help with statistics. This project has received funding
from the European Research Council (ERC) under the European Union''s Horizon 2020
research and innovation program (grant agreement No 742985). A.J. is supported by
funding from the Austrian Science Fund (FWF): I3630B25 to J.F. '
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: E
full_name: Himschoot, E
last_name: Himschoot
- first_name: R
full_name: Wang, R
last_name: Wang
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: J
full_name: Sánchez-Simarro, J
last_name: Sánchez-Simarro
- first_name: F
full_name: Aniento, F
last_name: Aniento
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Gallei MC, Tan S, et al. Systematic analysis of specific and
nonspecific auxin effects on endocytosis and trafficking. Plant Physiology.
2021;186(2):1122–1142. doi:10.1093/plphys/kiab134
apa: Narasimhan, M., Gallei, M. C., Tan, S., Johnson, A. J., Verstraeten, I., Li,
L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin
effects on endocytosis and trafficking. Plant Physiology. Oxford University
Press. https://doi.org/10.1093/plphys/kiab134
chicago: Narasimhan, Madhumitha, Michelle C Gallei, Shutang Tan, Alexander J Johnson,
Inge Verstraeten, Lanxin Li, Lesia Rodriguez Solovey, et al. “Systematic Analysis
of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” Plant
Physiology. Oxford University Press, 2021. https://doi.org/10.1093/plphys/kiab134.
ieee: M. Narasimhan et al., “Systematic analysis of specific and nonspecific
auxin effects on endocytosis and trafficking,” Plant Physiology, vol. 186,
no. 2. Oxford University Press, pp. 1122–1142, 2021.
ista: Narasimhan M, Gallei MC, Tan S, Johnson AJ, Verstraeten I, Li L, Rodriguez
Solovey L, Han H, Himschoot E, Wang R, Vanneste S, Sánchez-Simarro J, Aniento
F, Adamowski M, Friml J. 2021. Systematic analysis of specific and nonspecific
auxin effects on endocytosis and trafficking. Plant Physiology. 186(2), 1122–1142.
mla: Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific
Auxin Effects on Endocytosis and Trafficking.” Plant Physiology, vol. 186,
no. 2, Oxford University Press, 2021, pp. 1122–1142, doi:10.1093/plphys/kiab134.
short: M. Narasimhan, M.C. Gallei, S. Tan, A.J. Johnson, I. Verstraeten, L. Li,
L. Rodriguez Solovey, H. Han, E. Himschoot, R. Wang, S. Vanneste, J. Sánchez-Simarro,
F. Aniento, M. Adamowski, J. Friml, Plant Physiology 186 (2021) 1122–1142.
date_created: 2021-03-26T12:08:38Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2024-10-29T10:22:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plphys/kiab134
ec_funded: 1
external_id:
isi:
- '000671555900031'
pmid:
- '33734402'
file:
- access_level: open_access
checksum: 532bb9469d3b665907f06df8c383eade
content_type: application/pdf
creator: cziletti
date_created: 2021-11-11T15:07:51Z
date_updated: 2021-11-11T15:07:51Z
file_id: '10273'
file_name: 2021_PlantPhysio_Narasimhan.pdf
file_size: 2289127
relation: main_file
success: 1
file_date_updated: 2021-11-11T15:07:51Z
has_accepted_license: '1'
intvolume: ' 186'
isi: 1
issue: '2'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1122–1142
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: 10.1093/plphys/kiab380
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Systematic analysis of specific and nonspecific auxin effects on endocytosis
and trafficking
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 186
year: '2021'
...
---
_id: '7142'
abstract:
- lang: eng
text: The phytohormone auxin acts as an amazingly versatile coordinator of plant
growth and development. With its morphogen-like properties, auxin controls sites
and timing of differentiation and/or growth responses both, in quantitative and
qualitative terms. Specificity in the auxin response depends largely on distinct
modes of signal transmission, by which individual cells perceive and convert auxin
signals into a remarkable diversity of responses. The best understood, or so-called
canonical mechanism of auxin perception ultimately results in variable adjustments
of the cellular transcriptome, via a short, nuclear signal transduction pathway.
Additional findings that accumulated over decades implied that an additional,
presumably, cell surface-based auxin perception mechanism mediates very rapid
cellular responses and decisively contributes to the cell's overall hormonal response.
Recent investigations into both, nuclear and cell surface auxin signalling challenged
this assumed partition of roles for different auxin signalling pathways and revealed
an unexpected complexity in transcriptional and non-transcriptional cellular responses
mediated by auxin.
acknowledgement: Research in J.F. laboratory is funded by the European Union's Horizon
2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian
Science Fund (FWF grant P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 2020;53(2):43-49.
doi:10.1016/j.pbi.2019.10.003'
apa: 'Gallei, M. C., Luschnig, C., & Friml, J. (2020). Auxin signalling in growth:
Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2019.10.003'
chicago: 'Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling
in Growth: Schrödinger’s Cat out of the Bag.” Current Opinion in Plant Biology.
Elsevier, 2020. https://doi.org/10.1016/j.pbi.2019.10.003.'
ieee: 'M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s
cat out of the bag,” Current Opinion in Plant Biology, vol. 53, no. 2.
Elsevier, pp. 43–49, 2020.'
ista: 'Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.'
mla: 'Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat
out of the Bag.” Current Opinion in Plant Biology, vol. 53, no. 2, Elsevier,
2020, pp. 43–49, doi:10.1016/j.pbi.2019.10.003.'
short: M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020)
43–49.
date_created: 2019-12-02T12:05:26Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:07:22Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.10.003
ec_funded: 1
external_id:
isi:
- '000521120600007'
pmid:
- '31760231'
intvolume: ' 53'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 43-49
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
eissn:
- 1879-0356
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Auxin signalling in growth: Schrödinger''s cat out of the bag'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 53
year: '2020'
...
---
_id: '7465'
abstract:
- lang: eng
text: The flexible development of plants is characterized by a high capacity for
post-embryonic organ formation and tissue regeneration, processes, which require
tightly regulated intercellular communication and coordinated tissue (re-)polarization.
The phytohormone auxin, the main driver for these processes, is able to establish
polarized auxin transport channels, which are characterized by the expression
and polar, subcellular localization of the PIN1 auxin transport proteins. These
channels are demarcating the position of future vascular strands necessary for
organ formation and tissue regeneration. Major progress has been made in the last
years to understand how PINs can change their polarity in different contexts and
thus guide auxin flow through the plant. However, it still remains elusive how
auxin mediates the establishment of auxin conducting channels and the formation
of vascular tissue and which cellular processes are involved. By the means of
sophisticated regeneration experiments combined with local auxin applications
in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics,
(ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact
actin cytoskeleton required for post-endocytic trafficking are indispensable for
auxin channel formation, de novo vascular formation and vascular regeneration
after wounding. These observations provide novel insights into cellular mechanism
of coordinated tissue polarization during auxin canalization.
article_number: '110414'
article_processing_charge: No
article_type: original
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hélène S.
full_name: Robert, Hélène S.
last_name: Robert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 2020;293(4). doi:10.1016/j.plantsci.2020.110414
apa: Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., & Friml,
J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for
auxin canalization and vascular tissue formation in Arabidopsis. Plant Science.
Elsevier. https://doi.org/10.1016/j.plantsci.2020.110414
chicago: Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S.
Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are
Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.”
Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.plantsci.2020.110414.
ieee: E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml,
“Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis,” Plant Science, vol. 293,
no. 4. Elsevier, 2020.
ista: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 293(4), 110414.
mla: Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required
for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” Plant
Science, vol. 293, no. 4, 110414, Elsevier, 2020, doi:10.1016/j.plantsci.2020.110414.
short: E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant
Science 293 (2020).
date_created: 2020-02-09T23:00:50Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:37:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2020.110414
ec_funded: 1
external_id:
isi:
- '000520609800009'
file:
- access_level: open_access
checksum: f7f27c6a8fea985ceb9279be2204461c
content_type: application/pdf
creator: dernst
date_created: 2020-02-10T08:59:36Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7471'
file_name: 2020_PlantScience_Mazur.pdf
file_size: 3499069
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 293'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Science
publication_identifier:
eissn:
- '18732259'
issn:
- '01689452'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 293
year: '2020'
...
---
_id: '8138'
abstract:
- lang: eng
text: Directional transport of the phytohormone auxin is a versatile, plant-specific
mechanism regulating many aspects of plant development. The recently identified
plant hormones, strigolactones (SLs), are implicated in many plant traits; among
others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters
for fine-tuning of growth and developmental responses. Here, we show in pea and
Arabidopsis that SLs target processes dependent on the canalization of auxin flow,
which involves auxin feedback on PIN subcellular distribution. D14 receptor- and
MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels
after wounding or from artificial auxin sources, during vasculature de novo formation
and regeneration. At the cellular level, SLs interfere with auxin effects on PIN
polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis.
Our results identify a non-transcriptional mechanism of SL action, uncoupling
auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue
formation and regeneration.
acknowledgement: We are grateful to David Nelson for providing published materials
and extremely helpful comments, and Elizabeth Dun and Christine Beveridge for helpful
discussions. The research leading to these results has received funding from the
European Research Council (ERC) under the European Union's Horizon 2020 research
and innovation programme (742985). This work was also supported by the Beijing Municipal
Natural Science Foundation (5192011), Beijing Outstanding University Discipline
Program, the National Natural Science Foundation of China (31370309), CEITEC 2020
(LQ1601) project with financial contribution made by the Ministry of Education,
Youth and Sports of the Czech Republic within special support paid from the National
Program of Sustainability II funds, Australian Research Council (FT180100081), and
China Postdoctoral Science Foundation (2019M660864).
article_processing_charge: No
article_type: original
author:
- first_name: J
full_name: Zhang, J
last_name: Zhang
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: J
full_name: Balla, J
last_name: Balla
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: P
full_name: Kalousek, P
last_name: Kalousek
- first_name: Z
full_name: Medveďová, Z
last_name: Medveďová
- first_name: Y
full_name: Li, Y
last_name: Li
- first_name: Y
full_name: Wang, Y
last_name: Wang
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: V
full_name: Reinöhl, V
last_name: Reinöhl
- first_name: S
full_name: Procházka, S
last_name: Procházka
- first_name: R
full_name: Halouzka, R
last_name: Halouzka
- first_name: P
full_name: Tarkowski, P
last_name: Tarkowski
- first_name: C
full_name: Luschnig, C
last_name: Luschnig
- first_name: PB
full_name: Brewer, PB
last_name: Brewer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang J, Mazur E, Balla J, et al. Strigolactones inhibit auxin feedback on
PIN-dependent auxin transport canalization. Nature Communications. 2020;11(1):3508.
doi:10.1038/s41467-020-17252-y
apa: Zhang, J., Mazur, E., Balla, J., Gallei, M. C., Kalousek, P., Medveďová, Z.,
… Friml, J. (2020). Strigolactones inhibit auxin feedback on PIN-dependent auxin
transport canalization. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-17252-y
chicago: Zhang, J, E Mazur, J Balla, Michelle C Gallei, P Kalousek, Z Medveďová,
Y Li, et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport
Canalization.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-17252-y.
ieee: J. Zhang et al., “Strigolactones inhibit auxin feedback on PIN-dependent
auxin transport canalization,” Nature Communications, vol. 11, no. 1. Springer
Nature, p. 3508, 2020.
ista: Zhang J, Mazur E, Balla J, Gallei MC, Kalousek P, Medveďová Z, Li Y, Wang
Y, Prat T, Vasileva MK, Reinöhl V, Procházka S, Halouzka R, Tarkowski P, Luschnig
C, Brewer P, Friml J. 2020. Strigolactones inhibit auxin feedback on PIN-dependent
auxin transport canalization. Nature Communications. 11(1), 3508.
mla: Zhang, J., et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin
Transport Canalization.” Nature Communications, vol. 11, no. 1, Springer
Nature, 2020, p. 3508, doi:10.1038/s41467-020-17252-y.
short: J. Zhang, E. Mazur, J. Balla, M.C. Gallei, P. Kalousek, Z. Medveďová, Y.
Li, Y. Wang, T. Prat, M.K. Vasileva, V. Reinöhl, S. Procházka, R. Halouzka, P.
Tarkowski, C. Luschnig, P. Brewer, J. Friml, Nature Communications 11 (2020) 3508.
date_created: 2020-07-21T08:58:07Z
date_published: 2020-07-14T00:00:00Z
date_updated: 2023-08-22T08:13:44Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-020-17252-y
ec_funded: 1
external_id:
isi:
- '000550062200004'
pmid:
- '32665554'
file:
- access_level: open_access
content_type: application/pdf
creator: dernst
date_created: 2020-07-22T08:32:55Z
date_updated: 2020-07-22T08:32:55Z
file_id: '8148'
file_name: 2020_NatureComm_Zhang.pdf
file_size: 1759490
relation: main_file
success: 1
file_date_updated: 2020-07-22T08:32:55Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '3508'
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '7182'
abstract:
- lang: eng
text: During infection pathogens secrete small molecules, termed effectors, to manipulate
and control the interaction with their specific hosts. Both the pathogen and the
plant are under high selective pressure to rapidly adapt and co-evolve in what
is usually referred to as molecular arms race. Components of the host’s immune
system form a network that processes information about molecules with a foreign
origin and damage-associated signals, integrating them with developmental and
abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding
leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction
networks have been extensively characterized. However, little is known on whether
pathogenic effectors form complexes to overcome plant immunity and promote disease.
Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces
effectors that target hubs in the immune network of the host cell. Here we assess
the capability of U. maydis effector candidates to interact with each other, which
may play a crucial role during the infection process. Using a systematic yeast-two-hybrid
approach and based on a preliminary pooled screen, we selected 63 putative effectors
for one-on-one matings with a library of nearly 300 effector candidates. We found
that 126 of these effector candidates interacted either with themselves or other
predicted effectors. Although the functional relevance of the observed interactions
remains elusive, we propose that the observed abundance in complex formation between
effectors adds an additional level of complexity to effector research and should
be taken into consideration when studying effector evolution and function. Based
on this fundamental finding, we suggest various scenarios which could evolutionarily
drive the formation and stabilization of an effector interactome.
article_number: '1437'
article_processing_charge: No
article_type: original
author:
- first_name: André
full_name: Alcântara, André
last_name: Alcântara
- first_name: Jason
full_name: Bosch, Jason
last_name: Bosch
- first_name: Fahimeh
full_name: Nazari, Fahimeh
last_name: Nazari
- first_name: Gesa
full_name: Hoffmann, Gesa
last_name: Hoffmann
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Simon
full_name: Uhse, Simon
last_name: Uhse
- first_name: Martin A.
full_name: Darino, Martin A.
last_name: Darino
- first_name: Toluwase
full_name: Olukayode, Toluwase
last_name: Olukayode
- first_name: Daniel
full_name: Reumann, Daniel
last_name: Reumann
- first_name: Laura
full_name: Baggaley, Laura
last_name: Baggaley
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive
effector-complex formation. Frontiers in Plant Science. 2019;10(11). doi:10.3389/fpls.2019.01437
apa: Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S.,
… Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex
formation. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2019.01437
chicago: Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle
C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals
Extensive Effector-Complex Formation.” Frontiers in Plant Science. Frontiers,
2019. https://doi.org/10.3389/fpls.2019.01437.
ieee: A. Alcântara et al., “Systematic Y2H screening reveals extensive effector-complex
formation,” Frontiers in Plant Science, vol. 10, no. 11. Frontiers, 2019.
ista: Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA,
Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals
extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.
mla: Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex
Formation.” Frontiers in Plant Science, vol. 10, no. 11, 1437, Frontiers,
2019, doi:10.3389/fpls.2019.01437.
short: A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A.
Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science
10 (2019).
date_created: 2019-12-15T23:00:43Z
date_published: 2019-11-14T00:00:00Z
date_updated: 2023-09-06T14:33:46Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2019.01437
external_id:
isi:
- '000499821700001'
pmid:
- '31803201'
file:
- access_level: open_access
checksum: 995aa838aec2064d93550de82b40bbd1
content_type: application/pdf
creator: dernst
date_created: 2019-12-16T07:58:43Z
date_updated: 2020-07-14T12:47:52Z
file_id: '7185'
file_name: 2019_FrontiersPlant_Alcantara.pdf
file_size: 1532505
relation: main_file
file_date_updated: 2020-07-14T12:47:52Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Systematic Y2H screening reveals extensive effector-complex formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2019'
...
---
_id: '6260'
abstract:
- lang: eng
text: Polar auxin transport plays a pivotal role in plant growth and development.
PIN auxin efflux carriers regulate directional auxin movement by establishing
local auxin maxima, minima, and gradients that drive multiple developmental processes
and responses to environmental signals. Auxin has been proposed to modulate its
own transport by regulating subcellular PIN trafficking via processes such as
clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further
investigated the mechanisms by which auxin affects PIN trafficking by screening
auxin analogs and identified pinstatic acid (PISA) as a positive modulator of
polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on
hypocotyl elongation and adventitious root formation via positive regulation of
auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN
accumulation at the cell surface by inhibiting PIN internalization from the plasma
membrane. This work demonstrates PISA to be a promising chemical tool to dissect
the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.
acknowledgement: "We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden
University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University
of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis
work was supported by the Ministry of Education, Culture, Sports, Science, and Technology
(Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology
and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.),
and the European Union’s Horizon2020 program (European Research Council grant agreement
no. 742985 to J.F.)."
article_processing_charge: No
article_type: original
author:
- first_name: A
full_name: Oochi, A
last_name: Oochi
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: K
full_name: Fukui, K
last_name: Fukui
- first_name: Y
full_name: Nakao, Y
last_name: Nakao
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: M
full_name: Quareshy, M
last_name: Quareshy
- first_name: K
full_name: Takahashi, K
last_name: Takahashi
- first_name: T
full_name: Kinoshita, T
last_name: Kinoshita
- first_name: SR
full_name: Harborough, SR
last_name: Harborough
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
- first_name: H
full_name: Kasahara, H
last_name: Kasahara
- first_name: RM
full_name: Napier, RM
last_name: Napier
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: KI
full_name: Hayashi, KI
last_name: Hayashi
citation:
ama: Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by
inhibiting PIN internalization. Plant Physiology. 2019;180(2):1152-1165.
doi:10.1104/pp.19.00201
apa: Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., …
Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN
internalization. Plant Physiology. ASPB. https://doi.org/10.1104/pp.19.00201
chicago: Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy,
K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology. ASPB, 2019. https://doi.org/10.1104/pp.19.00201.
ieee: A. Oochi et al., “Pinstatic acid promotes auxin transport by inhibiting
PIN internalization,” Plant Physiology, vol. 180, no. 2. ASPB, pp. 1152–1165,
2019.
ista: Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita
T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic
acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology.
180(2), 1152–1165.
mla: Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology, vol. 180, no. 2, ASPB, 2019, pp. 1152–65,
doi:10.1104/pp.19.00201.
short: A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi,
T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K.
Hayashi, Plant Physiology 180 (2019) 1152–1165.
date_created: 2019-04-09T08:38:20Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.19.00201
ec_funded: 1
external_id:
isi:
- '000470086100045'
pmid:
- '30936248'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.19.00201
month: '06'
oa: 1
oa_version: Published Version
page: 1152-1165
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Pinstatic acid promotes auxin transport by inhibiting PIN internalization
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '104'
abstract:
- lang: eng
text: The biotrophic pathogen Ustilago maydis, the causative agent of corn smut
disease, infects one of the most important crops worldwide – Zea mays. To successfully
colonize its host, U. maydis secretes proteins, known as effectors, that suppress
plant defense responses and facilitate the establishment of biotrophy. In this
work, we describe the U. maydis effector protein Cce1. Cce1 is essential for virulence
and is upregulated during infection. Through microscopic analysis and in vitro
assays, we show that Cce1 is secreted from hyphae during filamentous growth of
the fungus. Strikingly, Δcce1 mutants are blocked at early stages of infection
and induce callose deposition as a plant defense response. Cce1 is highly conserved
among smut fungi and the Ustilago bromivora ortholog complemented the virulence
defect of the SG200Δcce1 deletion strain. These data indicate that Cce1 is a core
effector with apoplastic localization that is essential for U. maydis to infect
its host.
acknowledgement: 'the Austrian Science Fund (FWF): [P27429‐B22, P27818‐B22, I 3033‐B22],
and the Austrian Academy of Science (OEAW).'
article_processing_charge: No
author:
- first_name: Denise
full_name: Seitner, Denise
last_name: Seitner
- first_name: Simon
full_name: Uhse, Simon
last_name: Uhse
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Seitner D, Uhse S, Gallei MC, Djamei A. The core effector Cce1 is required
for early infection of maize by Ustilago maydis. Molecular Plant Pathology.
2018;19(10):2277-2287. doi:10.1111/mpp.12698
apa: Seitner, D., Uhse, S., Gallei, M. C., & Djamei, A. (2018). The core effector
Cce1 is required for early infection of maize by Ustilago maydis. Molecular
Plant Pathology. Wiley. https://doi.org/10.1111/mpp.12698
chicago: Seitner, Denise, Simon Uhse, Michelle C Gallei, and Armin Djamei. “The
Core Effector Cce1 Is Required for Early Infection of Maize by Ustilago Maydis.”
Molecular Plant Pathology. Wiley, 2018. https://doi.org/10.1111/mpp.12698.
ieee: D. Seitner, S. Uhse, M. C. Gallei, and A. Djamei, “The core effector Cce1
is required for early infection of maize by Ustilago maydis,” Molecular Plant
Pathology, vol. 19, no. 10. Wiley, pp. 2277–2287, 2018.
ista: Seitner D, Uhse S, Gallei MC, Djamei A. 2018. The core effector Cce1 is required
for early infection of maize by Ustilago maydis. Molecular Plant Pathology. 19(10),
2277–2287.
mla: Seitner, Denise, et al. “The Core Effector Cce1 Is Required for Early Infection
of Maize by Ustilago Maydis.” Molecular Plant Pathology, vol. 19, no. 10,
Wiley, 2018, pp. 2277–87, doi:10.1111/mpp.12698.
short: D. Seitner, S. Uhse, M.C. Gallei, A. Djamei, Molecular Plant Pathology 19
(2018) 2277–2287.
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