[{"title":"ABP1–TMK auxin perception for global phosphorylation and auxin canalization","date_updated":"2023-11-07T08:16:09Z","oa":1,"issue":"7927","article_processing_charge":"No","has_accepted_license":"1","intvolume":"       609","scopus_import":"1","isi":1,"language":[{"iso":"eng"}],"publication":"Nature","author":[{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei"},{"orcid":"0000-0003-4783-1752","last_name":"Gelová","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","first_name":"Zuzana","full_name":"Gelová, Zuzana"},{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","full_name":"Johnson, Alexander J","first_name":"Alexander J"},{"first_name":"Ewa","full_name":"Mazur, Ewa","last_name":"Mazur"},{"first_name":"Aline","full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer"},{"full_name":"Rodriguez Solovey, Lesia","first_name":"Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237"},{"last_name":"Roosjen","full_name":"Roosjen, Mark","first_name":"Mark"},{"full_name":"Verstraeten, Inge","first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","orcid":"0000-0001-7241-2328"},{"first_name":"Branka D.","full_name":"Živanović, Branka D.","last_name":"Živanović"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou","first_name":"Minxia","full_name":"Zou, Minxia"},{"id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler","first_name":"Lukas","full_name":"Fiedler, Lukas"},{"id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini","first_name":"Caterina","full_name":"Giannini, Caterina"},{"last_name":"Grones","full_name":"Grones, Peter","first_name":"Peter"},{"last_name":"Hrtyan","id":"45A71A74-F248-11E8-B48F-1D18A9856A87","full_name":"Hrtyan, Mónika","first_name":"Mónika"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","orcid":"0000-0001-9735-5315","first_name":"Walter","full_name":"Kaufmann, Walter"},{"full_name":"Kuhn, Andre","first_name":"Andre","last_name":"Kuhn"},{"last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671","full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha"},{"last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","first_name":"Marek","full_name":"Randuch, Marek"},{"last_name":"Rýdza","first_name":"Nikola","full_name":"Rýdza, Nikola"},{"last_name":"Takahashi","full_name":"Takahashi, Koji","first_name":"Koji"},{"last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285","first_name":"Shutang","full_name":"Tan, Shutang"},{"id":"e3736151-106c-11ec-b916-c2558e2762c6","last_name":"Teplova","first_name":"Anastasiia","full_name":"Teplova, Anastasiia"},{"last_name":"Kinoshita","full_name":"Kinoshita, Toshinori","first_name":"Toshinori"},{"last_name":"Weijers","first_name":"Dolf","full_name":"Weijers, Dolf"},{"first_name":"Hana","full_name":"Rakusová, Hana","last_name":"Rakusová"}],"oa_version":"Submitted Version","day":"15","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"creator":"amally","access_level":"open_access","file_size":79774945,"content_type":"application/pdf","relation":"main_file","success":1,"file_name":"Friml Nature 2022_merged.pdf","file_id":"14483","checksum":"a6055c606aefb900bf62ae3e7d15f921","date_created":"2023-11-02T17:12:37Z","date_updated":"2023-11-02T17:12:37Z"}],"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"month":"09","pmid":1,"status":"public","type":"journal_article","citation":{"apa":"Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>","chicago":"Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>.","mla":"Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>.","ista":"Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.","short":"J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581.","ieee":"J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022.","ama":"Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>"},"date_created":"2023-01-16T10:04:48Z","acknowledgement":"We acknowledge K. Kubiasová for excellent technical assistance, J. Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI; and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F. is grateful to R. Napier for many insightful suggestions and support. We thank all past and present members of the Friml group for their support and for other contributions to this effort to clarify the controversial role of ABP1 over the past seven years. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.); the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001 to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053 to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910).","year":"2022","ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"file_date_updated":"2023-11-02T17:12:37Z","_id":"12291","article_type":"original","doi":"10.1038/s41586-022-05187-x","publication_status":"published","external_id":{"isi":["000851357500002"],"pmid":["36071161"]},"abstract":[{"text":"The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization.","lang":"eng"}],"ddc":["580"],"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"grant_number":"P29988","_id":"262EF96E-B435-11E9-9278-68D0E5697425","name":"RNA-directed DNA methylation in plant development","call_identifier":"FWF"}],"page":"575-581","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"EvBe"},{"_id":"EM-Fac"}],"volume":609,"quality_controlled":"1","publisher":"Springer Nature","date_published":"2022-09-15T00:00:00Z"},{"department":[{"_id":"JiFr"}],"page":"440-449","volume":27,"quality_controlled":"1","publisher":"Cell Press","date_published":"2022-05-01T00:00:00Z","project":[{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","_id":"26B4D67E-B435-11E9-9278-68D0E5697425","grant_number":"25351"}],"external_id":{"isi":["000793707900005"],"pmid":["34848141"]},"abstract":[{"text":"The phytohormone auxin is the major growth regulator governing tropic responses including gravitropism. Auxin build-up at the lower side of stimulated shoots promotes cell expansion, whereas in roots it inhibits growth, leading to upward shoot bending and downward root bending, respectively. Yet it remains an enigma how the same signal can trigger such opposite cellular responses. In this review, we discuss several recent unexpected insights into the mechanisms underlying auxin regulation of growth, challenging several existing models. We focus on the divergent mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling pathways. From this emerges a more comprehensive, updated picture how auxin regulates growth.","lang":"eng"}],"ddc":["580"],"publication_status":"published","doi":"10.1016/j.tplants.2021.11.006","file_date_updated":"2023-11-02T17:00:03Z","article_type":"original","_id":"10411","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11626"}]},"citation":{"apa":"Li, L., Gallei, M. C., &#38; Friml, J. (2022). Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>","mla":"Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>.","chicago":"Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>.","ista":"Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms. Trends in Plant Science. 27(5), 440–449.","ieee":"L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for tropisms,” <i>Trends in Plant Science</i>, vol. 27, no. 5. Cell Press, pp. 440–449, 2022.","ama":"Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. 2022;27(5):440-449. doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>","short":"L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449."},"date_created":"2021-12-05T23:01:43Z","acknowledgement":"The authors thank Alexandra Mally for editing the text. This work was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were created with BioRender.com.","year":"2022","pmid":1,"status":"public","type":"journal_article","oa_version":"Submitted Version","day":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"content_type":"application/pdf","access_level":"open_access","file_size":805779,"relation":"main_file","creator":"amally","date_updated":"2023-11-02T17:00:03Z","date_created":"2023-11-02T17:00:03Z","file_id":"14480","file_name":"Li Plants 2021_accepted.pdf","checksum":"3d94980ee1ff6bec100dd813f6a921a6","success":1}],"publication_identifier":{"issn":["1360-1385"]},"month":"05","author":[{"full_name":"Li, Lanxin","first_name":"Lanxin","orcid":"0000-0002-5607-272X","last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michelle C","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","orcid":"0000-0003-1286-7368"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"publication":"Trends in Plant Science","language":[{"iso":"eng"}],"has_accepted_license":"1","intvolume":"        27","scopus_import":"1","isi":1,"title":"Bending to auxin: Fast acid growth for tropisms","oa":1,"date_updated":"2024-10-29T10:12:33Z","issue":"5","article_processing_charge":"No"},{"year":"2022","citation":{"apa":"Gallei, M. C. (2022). <i>Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:11626\">https://doi.org/10.15479/at:ista:11626</a>","chicago":"Gallei, Michelle C. “Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2022. <a href=\"https://doi.org/10.15479/at:ista:11626\">https://doi.org/10.15479/at:ista:11626</a>.","ista":"Gallei MC. 2022. Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana. Institute of Science and Technology Austria.","mla":"Gallei, Michelle C. <i>Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana</i>. Institute of Science and Technology Austria, 2022, doi:<a href=\"https://doi.org/10.15479/at:ista:11626\">10.15479/at:ista:11626</a>.","short":"M.C. Gallei, Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2022.","ieee":"M. C. Gallei, “Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2022.","ama":"Gallei MC. Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana. 2022. doi:<a href=\"https://doi.org/10.15479/at:ista:11626\">10.15479/at:ista:11626</a>"},"date_created":"2022-07-20T11:21:53Z","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"9287"},{"status":"public","relation":"part_of_dissertation","id":"7142"},{"relation":"part_of_dissertation","id":"7465","status":"public"},{"relation":"part_of_dissertation","id":"8138","status":"public"},{"status":"public","id":"6260","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"8931"},{"status":"public","id":"10411","relation":"part_of_dissertation"}]},"degree_awarded":"PhD","ec_funded":1,"_id":"11626","file_date_updated":"2022-07-25T11:48:45Z","doi":"10.15479/at:ista:11626","publication_status":"published","ddc":["575"],"abstract":[{"text":"Plant growth and development is well known to be both, flexible and dynamic. The high capacity for post-embryonic organ formation and tissue regeneration requires tightly regulated intercellular communication and coordinated tissue polarization. One of the most important drivers for patterning and polarity in plant development is the phytohormone auxin. Auxin has the unique characteristic to establish polarized channels for its own active directional cell to cell transport. This fascinating phenomenon is called auxin canalization. Those auxin transport channels are characterized by the expression and polar, subcellular localization of PIN auxin efflux carriers. PIN proteins have the ability to dynamically change their localization and auxin itself can affect this by interfering with trafficking. Most of the underlying molecular mechanisms of canalization still remain enigmatic. What is known so far is that canonical auxin signaling is indispensable but also other non-canonical signaling components are thought to play a role. In order to shed light into the mysteries auf auxin canalization this study revisits the branches of auxin signaling in detail. Further a new auxin analogue, PISA, is developed which triggers auxin-like responses but does not directly activate canonical transcriptional auxin signaling. We revisit the direct auxin effect on PIN trafficking where we found that, contradictory to previous observations, auxin is very specifically promoting endocytosis of PIN2 but has no overall effect on endocytosis. Further, we evaluate which cellular processes related to PIN subcellular dynamics are involved in the establishment of auxin conducting channels and the formation of vascular tissue. We are re-evaluating the function of AUXIN BINDING PROTEIN 1 (ABP1) and provide a comprehensive picture about its developmental phneotypes and involvement in auxin signaling and canalization. Lastly, we are focusing on the crosstalk between the hormone strigolactone (SL) and auxin and found that SL is interfering with essentially all processes involved in auxin canalization in a non-transcriptional manner. Lastly we identify a new way of SL perception and signaling which is emanating from mitochondria, is independent of canonical SL signaling and is modulating primary root growth.","lang":"eng"}],"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"}],"supervisor":[{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"full_name":"Shani, Eilon","first_name":"Eilon","last_name":"Shani"}],"date_published":"2022-07-20T00:00:00Z","publisher":"Institute of Science and Technology Austria","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"page":"248","article_processing_charge":"No","date_updated":"2024-10-29T10:22:45Z","oa":1,"title":"Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana","has_accepted_license":"1","language":[{"iso":"eng"}],"author":[{"orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","full_name":"Gallei, Michelle C"}],"month":"07","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-019-0"]},"file":[{"date_created":"2022-07-25T09:08:47Z","date_updated":"2022-07-25T09:08:47Z","file_name":"Thesis_Gallei.pdf","checksum":"bd7ac35403cf5b4b2607287d2a104b3a","file_id":"11645","relation":"main_file","file_size":9730864,"content_type":"application/pdf","access_level":"open_access","creator":"mgallei"},{"date_updated":"2022-07-25T09:39:58Z","date_created":"2022-07-25T09:09:09Z","file_name":"Thesis_Gallei_source.docx","checksum":"a9e54fe5471ba25dc13c2150c1b8ccbb","file_id":"11646","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file","access_level":"closed","file_size":19560720,"creator":"mgallei"},{"file_size":24542837,"content_type":"application/pdf","access_level":"closed","relation":"source_file","creator":"mgallei","date_updated":"2022-07-25T09:39:58Z","date_created":"2022-07-25T09:09:32Z","file_name":"Thesis_Gallei_to_print.pdf","checksum":"3994f7f20058941b5bb8a16886b21e71","file_id":"11647","description":"This is the print version of the thesis including the full appendix"},{"date_updated":"2022-07-25T11:48:45Z","date_created":"2022-07-25T11:48:45Z","checksum":"f24acd3c0d864f4c6676e8b0d7bfa76b","file_name":"Thesis_Gallei_Appendix.pdf","file_id":"11650","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_size":15435966,"creator":"mgallei"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","oa_version":"Published Version","day":"20","status":"public","type":"dissertation","alternative_title":["ISTA Thesis"]},{"keyword":["Agronomy and Crop Science","Plant Science","Genetics","General Medicine"],"date_created":"2020-12-09T14:48:28Z","citation":{"ama":"Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. 2021;303. doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>","ieee":"Z. Gelová <i>et al.</i>, “Developmental roles of auxin binding protein 1 in Arabidopsis thaliana,” <i>Plant Science</i>, vol. 303. Elsevier, 2021.","short":"Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L. Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild, M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml, Plant Science 303 (2021).","ista":"Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.","mla":"Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>, vol. 303, 110750, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>.","chicago":"Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud, Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>.","apa":"Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M., … Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>"},"year":"2021","acknowledgement":"We would like to acknowledge Bioimaging and Life Science Facilities at IST Austria for continuous support and also the Plant Sciences Core Facility of CEITEC Masaryk University for their support with obtaining a part of the scientific data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct design. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program [grant agreement no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship of the Austrian Academy of Sciences to L.L.; the European Structural and Investment Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“ [CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738] to M. Č.","related_material":{"record":[{"relation":"dissertation_contains","id":"11626","status":"public"},{"relation":"dissertation_contains","id":"10083","status":"public"}]},"article_number":"110750","ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_type":"original","_id":"8931","file_date_updated":"2021-02-04T07:49:25Z","doi":"10.1016/j.plantsci.2020.110750","publication_status":"published","abstract":[{"lang":"eng","text":"Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear.\r\nHere we combined expression analysis with gain- and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy."}],"external_id":{"pmid":["33487339"],"isi":["000614154500001"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ddc":["580"],"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","_id":"26B4D67E-B435-11E9-9278-68D0E5697425","grant_number":"25351"}],"volume":303,"quality_controlled":"1","department":[{"_id":"JiFr"},{"_id":"Bio"}],"date_published":"2021-02-01T00:00:00Z","publisher":"Elsevier","title":"Developmental roles of auxin binding protein 1 in Arabidopsis thaliana","article_processing_charge":"Yes (via OA deal)","date_updated":"2024-10-29T10:22:43Z","oa":1,"has_accepted_license":"1","intvolume":"       303","scopus_import":"1","isi":1,"language":[{"iso":"eng"}],"publication":"Plant Science","author":[{"id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","last_name":"Gelová","orcid":"0000-0003-4783-1752","first_name":"Zuzana","full_name":"Gelová, Zuzana"},{"first_name":"Michelle C","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markéta","full_name":"Pernisová, Markéta","last_name":"Pernisová"},{"last_name":"Brunoud","full_name":"Brunoud, Géraldine","first_name":"Géraldine"},{"orcid":"0000-0001-7048-4627","last_name":"Zhang","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","first_name":"Xixi","full_name":"Zhang, Xixi"},{"first_name":"Matous","full_name":"Glanc, Matous","orcid":"0000-0003-0619-7783","last_name":"Glanc","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2"},{"id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li","orcid":"0000-0002-5607-272X","full_name":"Li, Lanxin","first_name":"Lanxin"},{"id":"483727CA-F248-11E8-B48F-1D18A9856A87","last_name":"Michalko","first_name":"Jaroslav","full_name":"Michalko, Jaroslav"},{"last_name":"Pavlovicova","full_name":"Pavlovicova, Zlata","first_name":"Zlata"},{"first_name":"Inge","full_name":"Verstraeten, Inge","last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328"},{"last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","full_name":"Han, Huibin"},{"first_name":"Jakub","full_name":"Hajny, Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","last_name":"Hajny","orcid":"0000-0003-2140-7195"},{"first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Čovanová, Milada","first_name":"Milada","last_name":"Čovanová"},{"full_name":"Zwiewka, Marta","first_name":"Marta","last_name":"Zwiewka"},{"first_name":"Lukas","full_name":"Hörmayer, Lukas","last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926"},{"id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych","orcid":"0000-0002-9767-8699","first_name":"Matyas","full_name":"Fendrych, Matyas"},{"last_name":"Xu","full_name":"Xu, Tongda","first_name":"Tongda"},{"first_name":"Teva","full_name":"Vernoux, Teva","last_name":"Vernoux"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"creator":"dernst","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_size":12563728,"file_name":"2021_PlantScience_Gelova.pdf","checksum":"a7f2562bdca62d67dfa88e271b62a629","file_id":"9083","success":1,"date_updated":"2021-02-04T07:49:25Z","date_created":"2021-02-04T07:49:25Z"}],"oa_version":"Published Version","day":"01","month":"02","publication_identifier":{"issn":["0168-9452"]},"status":"public","type":"journal_article","pmid":1},{"status":"public","type":"journal_article","pmid":1,"author":[{"full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha","last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei"},{"orcid":"0000-0002-0471-8285","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","full_name":"Tan, Shutang"},{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","first_name":"Alexander J","full_name":"Johnson, Alexander J"},{"full_name":"Verstraeten, Inge","first_name":"Inge","orcid":"0000-0001-7241-2328","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten"},{"id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li","orcid":"0000-0002-5607-272X","full_name":"Li, Lanxin","first_name":"Lanxin"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia"},{"first_name":"Huibin","full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","last_name":"Han"},{"full_name":"Himschoot, E","first_name":"E","last_name":"Himschoot"},{"last_name":"Wang","full_name":"Wang, R","first_name":"R"},{"last_name":"Vanneste","first_name":"S","full_name":"Vanneste, S"},{"first_name":"J","full_name":"Sánchez-Simarro, J","last_name":"Sánchez-Simarro"},{"full_name":"Aniento, F","first_name":"F","last_name":"Aniento"},{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek","first_name":"Maciek"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"file":[{"content_type":"application/pdf","file_size":2289127,"relation":"main_file","access_level":"open_access","creator":"cziletti","date_updated":"2021-11-11T15:07:51Z","date_created":"2021-11-11T15:07:51Z","file_name":"2021_PlantPhysio_Narasimhan.pdf","checksum":"532bb9469d3b665907f06df8c383eade","file_id":"10273","success":1}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","day":"01","month":"06","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"language":[{"iso":"eng"}],"publication":"Plant Physiology","title":"Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking","article_processing_charge":"Yes (in subscription journal)","issue":"2","date_updated":"2024-10-29T10:22:43Z","oa":1,"intvolume":"       186","has_accepted_license":"1","isi":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","volume":186,"page":"1122–1142","department":[{"_id":"JiFr"}],"date_published":"2021-06-01T00:00:00Z","publisher":"Oxford University Press","publication_status":"published","abstract":[{"text":"The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the\r\nauxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere with Brefeldin A-mediated intracellular PIN2 accumulation and also with general aggregation of endomembrane compartments. NAA treatment decreased the internalization of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the number, distribution, and compartment identity of the early endosome/trans-Golgi network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the endomembrane system, we opted for alternative approaches visualizing the endocytic events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the incidence and dynamics of clathrin foci, implying that these treatments do not affect the overall endocytosis rate. However, both NAA and IAA at low concentrations rapidly and specifically promoted endocytosis of photo-converted PIN2 from the PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis, thus contributing to its\r\npolarity maintenance and furthermore illustrate that high auxin levels have nonspecific effects on trafficking and endomembrane compartments. ","lang":"eng"}],"external_id":{"pmid":["33734402"],"isi":["000671555900031"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ddc":["580"],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"_id":"9287","article_type":"original","file_date_updated":"2021-11-11T15:07:51Z","doi":"10.1093/plphys/kiab134","date_created":"2021-03-26T12:08:38Z","citation":{"apa":"Narasimhan, M., Gallei, M. C., Tan, S., Johnson, A. J., Verstraeten, I., Li, L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. <i>Plant Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plphys/kiab134\">https://doi.org/10.1093/plphys/kiab134</a>","ieee":"M. Narasimhan <i>et al.</i>, “Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking,” <i>Plant Physiology</i>, vol. 186, no. 2. Oxford University Press, pp. 1122–1142, 2021.","ama":"Narasimhan M, Gallei MC, Tan S, et al. Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. <i>Plant Physiology</i>. 2021;186(2):1122–1142. doi:<a href=\"https://doi.org/10.1093/plphys/kiab134\">10.1093/plphys/kiab134</a>","short":"M. Narasimhan, M.C. Gallei, S. Tan, A.J. Johnson, I. Verstraeten, L. Li, L. Rodriguez Solovey, H. Han, E. Himschoot, R. Wang, S. Vanneste, J. Sánchez-Simarro, F. Aniento, M. Adamowski, J. Friml, Plant Physiology 186 (2021) 1122–1142.","mla":"Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” <i>Plant Physiology</i>, vol. 186, no. 2, Oxford University Press, 2021, pp. 1122–1142, doi:<a href=\"https://doi.org/10.1093/plphys/kiab134\">10.1093/plphys/kiab134</a>.","chicago":"Narasimhan, Madhumitha, Michelle C Gallei, Shutang Tan, Alexander J Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez Solovey, et al. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” <i>Plant Physiology</i>. Oxford University Press, 2021. <a href=\"https://doi.org/10.1093/plphys/kiab134\">https://doi.org/10.1093/plphys/kiab134</a>.","ista":"Narasimhan M, Gallei MC, Tan S, Johnson AJ, Verstraeten I, Li L, Rodriguez Solovey L, Han H, Himschoot E, Wang R, Vanneste S, Sánchez-Simarro J, Aniento F, Adamowski M, Friml J. 2021. Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiology. 186(2), 1122–1142."},"year":"2021","acknowledgement":"We thank Ivan Kulik for developing the Chip’n’Dale apparatus with Lanxin Li; the IST machine shop and the Bioimaging facility for their excellent support; Matouš Glanc and Matyáš Fendrych for their valuable discussions and help; Barbara Casillas-Perez for her help with statistics. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No 742985). A.J. is supported by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. ","related_material":{"link":[{"relation":"erratum","url":"10.1093/plphys/kiab380"}],"record":[{"status":"public","relation":"dissertation_contains","id":"11626"},{"id":"10083","relation":"dissertation_contains","status":"public"}]},"ec_funded":1},{"author":[{"orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","first_name":"Michelle C"},{"first_name":"Christian","full_name":"Luschnig, Christian","last_name":"Luschnig"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"oa_version":"None","day":"01","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"02","publication_identifier":{"issn":["1369-5266"],"eissn":["1879-0356"]},"pmid":1,"type":"journal_article","status":"public","title":"Auxin signalling in growth: Schrödinger's cat out of the bag","date_updated":"2023-08-17T14:07:22Z","issue":"2","article_processing_charge":"No","scopus_import":"1","intvolume":"        53","isi":1,"language":[{"iso":"eng"}],"publication":"Current Opinion in Plant Biology","publication_status":"published","external_id":{"isi":["000521120600007"],"pmid":["31760231"]},"abstract":[{"text":"The phytohormone auxin acts as an amazingly versatile coordinator of plant growth and development. With its morphogen-like properties, auxin controls sites and timing of differentiation and/or growth responses both, in quantitative and qualitative terms. Specificity in the auxin response depends largely on distinct modes of signal transmission, by which individual cells perceive and convert auxin signals into a remarkable diversity of responses. The best understood, or so-called canonical mechanism of auxin perception ultimately results in variable adjustments of the cellular transcriptome, via a short, nuclear signal transduction pathway. Additional findings that accumulated over decades implied that an additional, presumably, cell surface-based auxin perception mechanism mediates very rapid cellular responses and decisively contributes to the cell's overall hormonal response. Recent investigations into both, nuclear and cell surface auxin signalling challenged this assumed partition of roles for different auxin signalling pathways and revealed an unexpected complexity in transcriptional and non-transcriptional cellular responses mediated by auxin.","lang":"eng"}],"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"department":[{"_id":"JiFr"}],"page":"43-49","volume":53,"quality_controlled":"1","publisher":"Elsevier","date_published":"2020-02-01T00:00:00Z","date_created":"2019-12-02T12:05:26Z","citation":{"chicago":"Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>.","ista":"Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.","mla":"Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2, Elsevier, 2020, pp. 43–49, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>.","short":"M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020) 43–49.","ieee":"M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s cat out of the bag,” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2. Elsevier, pp. 43–49, 2020.","ama":"Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. 2020;53(2):43-49. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>","apa":"Gallei, M. C., Luschnig, C., &#38; Friml, J. (2020). Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>"},"acknowledgement":"Research in J.F. laboratory is funded by the European Union's Horizon 2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian Science Fund (FWF grant P 31493).","year":"2020","ec_funded":1,"related_material":{"record":[{"id":"11626","relation":"dissertation_contains","status":"public"}]},"article_type":"original","_id":"7142","doi":"10.1016/j.pbi.2019.10.003"},{"status":"public","type":"journal_article","month":"04","publication_identifier":{"issn":["01689452"],"eissn":["18732259"]},"oa_version":"Published Version","day":"01","file":[{"content_type":"application/pdf","access_level":"open_access","file_size":3499069,"relation":"main_file","creator":"dernst","date_created":"2020-02-10T08:59:36Z","date_updated":"2020-07-14T12:47:59Z","checksum":"f7f27c6a8fea985ceb9279be2204461c","file_name":"2020_PlantScience_Mazur.pdf","file_id":"7471"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"last_name":"Mazur","full_name":"Mazur, Ewa","first_name":"Ewa"},{"orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","first_name":"Michelle C","full_name":"Gallei, Michelle C"},{"first_name":"Maciek","full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski","orcid":"0000-0001-6463-5257"},{"last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","first_name":"Huibin"},{"first_name":"Hélène S.","full_name":"Robert, Hélène S.","last_name":"Robert"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publication":"Plant Science","language":[{"iso":"eng"}],"isi":1,"has_accepted_license":"1","intvolume":"       293","scopus_import":"1","date_updated":"2023-08-17T14:37:32Z","oa":1,"article_processing_charge":"No","issue":"4","title":"Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis","publisher":"Elsevier","date_published":"2020-04-01T00:00:00Z","department":[{"_id":"JiFr"}],"quality_controlled":"1","volume":293,"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"ddc":["580"],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"external_id":{"isi":["000520609800009"]},"abstract":[{"text":"The flexible development of plants is characterized by a high capacity for post-embryonic organ formation and tissue regeneration, processes, which require tightly regulated intercellular communication and coordinated tissue (re-)polarization. The phytohormone auxin, the main driver for these processes, is able to establish polarized auxin transport channels, which are characterized by the expression and polar, subcellular localization of the PIN1 auxin transport proteins. These channels are demarcating the position of future vascular strands necessary for organ formation and tissue regeneration. Major progress has been made in the last years to understand how PINs can change their polarity in different contexts and thus guide auxin flow through the plant. However, it still remains elusive how auxin mediates the establishment of auxin conducting channels and the formation of vascular tissue and which cellular processes are involved. By the means of sophisticated regeneration experiments combined with local auxin applications in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics, (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact actin cytoskeleton required for post-endocytic trafficking are indispensable for auxin channel formation, de novo vascular formation and vascular regeneration after wounding. These observations provide novel insights into cellular mechanism of coordinated tissue polarization during auxin canalization.","lang":"eng"}],"publication_status":"published","doi":"10.1016/j.plantsci.2020.110414","file_date_updated":"2020-07-14T12:47:59Z","article_type":"original","_id":"7465","ec_funded":1,"article_number":"110414","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11626"}]},"year":"2020","date_created":"2020-02-09T23:00:50Z","citation":{"apa":"Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., &#38; Friml, J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">https://doi.org/10.1016/j.plantsci.2020.110414</a>","short":"E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant Science 293 (2020).","ama":"Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. 2020;293(4). doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">10.1016/j.plantsci.2020.110414</a>","ieee":"E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml, “Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis,” <i>Plant Science</i>, vol. 293, no. 4. Elsevier, 2020.","mla":"Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>, vol. 293, no. 4, 110414, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">10.1016/j.plantsci.2020.110414</a>.","ista":"Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. Plant Science. 293(4), 110414.","chicago":"Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S. Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">https://doi.org/10.1016/j.plantsci.2020.110414</a>."}},{"title":"Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization","article_processing_charge":"No","issue":"1","oa":1,"date_updated":"2023-08-22T08:13:44Z","intvolume":"        11","scopus_import":"1","has_accepted_license":"1","isi":1,"language":[{"iso":"eng"}],"publication":"Nature Communications","author":[{"first_name":"J","full_name":"Zhang, J","last_name":"Zhang"},{"last_name":"Mazur","first_name":"E","full_name":"Mazur, E"},{"first_name":"J","full_name":"Balla, J","last_name":"Balla"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","orcid":"0000-0003-1286-7368","first_name":"Michelle C","full_name":"Gallei, Michelle C"},{"full_name":"Kalousek, P","first_name":"P","last_name":"Kalousek"},{"last_name":"Medveďová","full_name":"Medveďová, Z","first_name":"Z"},{"last_name":"Li","first_name":"Y","full_name":"Li, Y"},{"full_name":"Wang, Y","first_name":"Y","last_name":"Wang"},{"last_name":"Prat","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","first_name":"Tomas","full_name":"Prat, Tomas"},{"id":"3407EB18-F248-11E8-B48F-1D18A9856A87","last_name":"Vasileva","first_name":"Mina K","full_name":"Vasileva, Mina K"},{"first_name":"V","full_name":"Reinöhl, V","last_name":"Reinöhl"},{"last_name":"Procházka","full_name":"Procházka, S","first_name":"S"},{"last_name":"Halouzka","first_name":"R","full_name":"Halouzka, R"},{"last_name":"Tarkowski","first_name":"P","full_name":"Tarkowski, P"},{"last_name":"Luschnig","first_name":"C","full_name":"Luschnig, C"},{"first_name":"PB","full_name":"Brewer, PB","last_name":"Brewer"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"date_created":"2020-07-22T08:32:55Z","date_updated":"2020-07-22T08:32:55Z","success":1,"file_name":"2020_NatureComm_Zhang.pdf","file_id":"8148","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_size":1759490,"creator":"dernst"}],"day":"14","oa_version":"Published Version","publication_identifier":{"issn":["2041-1723"]},"month":"07","status":"public","type":"journal_article","pmid":1,"date_created":"2020-07-21T08:58:07Z","citation":{"mla":"Zhang, J., et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>, vol. 11, no. 1, Springer Nature, 2020, p. 3508, doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>.","ista":"Zhang J, Mazur E, Balla J, Gallei MC, Kalousek P, Medveďová Z, Li Y, Wang Y, Prat T, Vasileva MK, Reinöhl V, Procházka S, Halouzka R, Tarkowski P, Luschnig C, Brewer P, Friml J. 2020. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. Nature Communications. 11(1), 3508.","chicago":"Zhang, J, E Mazur, J Balla, Michelle C Gallei, P Kalousek, Z Medveďová, Y Li, et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>.","ama":"Zhang J, Mazur E, Balla J, et al. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. 2020;11(1):3508. doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>","ieee":"J. Zhang <i>et al.</i>, “Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization,” <i>Nature Communications</i>, vol. 11, no. 1. Springer Nature, p. 3508, 2020.","short":"J. Zhang, E. Mazur, J. Balla, M.C. Gallei, P. Kalousek, Z. Medveďová, Y. Li, Y. Wang, T. Prat, M.K. Vasileva, V. Reinöhl, S. Procházka, R. Halouzka, P. Tarkowski, C. Luschnig, P. Brewer, J. Friml, Nature Communications 11 (2020) 3508.","apa":"Zhang, J., Mazur, E., Balla, J., Gallei, M. C., Kalousek, P., Medveďová, Z., … Friml, J. (2020). Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>"},"year":"2020","acknowledgement":"We are grateful to David Nelson for providing published materials and extremely helpful comments, and Elizabeth Dun and Christine Beveridge for helpful discussions. The research leading to these results has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (742985). This work was also supported by the Beijing Municipal Natural Science Foundation (5192011), Beijing Outstanding University Discipline Program, the National Natural Science Foundation of China (31370309), CEITEC 2020 (LQ1601) project with financial contribution made by the Ministry of Education, Youth and Sports of the Czech Republic within special support paid from the National Program of Sustainability II funds, Australian Research Council (FT180100081), and China Postdoctoral Science Foundation (2019M660864).","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11626"}]},"ec_funded":1,"_id":"8138","article_type":"original","file_date_updated":"2020-07-22T08:32:55Z","doi":"10.1038/s41467-020-17252-y","publication_status":"published","abstract":[{"text":"Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxin effects on PIN polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis. Our results identify a non-transcriptional mechanism of SL action, uncoupling auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue formation and regeneration.","lang":"eng"}],"external_id":{"pmid":["32665554"],"isi":["000550062200004"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ddc":["580"],"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","volume":11,"department":[{"_id":"JiFr"}],"page":"3508","date_published":"2020-07-14T00:00:00Z","publisher":"Springer Nature"},{"doi":"10.3389/fpls.2019.01437","file_date_updated":"2020-07-14T12:47:52Z","_id":"7182","article_type":"original","article_number":"1437","year":"2019","date_created":"2019-12-15T23:00:43Z","citation":{"short":"A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A. Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science 10 (2019).","ieee":"A. Alcântara <i>et al.</i>, “Systematic Y2H screening reveals extensive effector-complex formation,” <i>Frontiers in Plant Science</i>, vol. 10, no. 11. Frontiers, 2019.","ama":"Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. 2019;10(11). doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>","mla":"Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>, vol. 10, no. 11, 1437, Frontiers, 2019, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>.","ista":"Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA, Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.","chicago":"Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>. Frontiers, 2019. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>.","apa":"Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S., … Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. Frontiers. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>"},"publisher":"Frontiers","date_published":"2019-11-14T00:00:00Z","department":[{"_id":"JiFr"}],"volume":10,"quality_controlled":"1","ddc":["580"],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"external_id":{"pmid":["31803201"],"isi":["000499821700001"]},"abstract":[{"lang":"eng","text":"During infection pathogens secrete small molecules, termed effectors, to manipulate and control the interaction with their specific hosts. Both the pathogen and the plant are under high selective pressure to rapidly adapt and co-evolve in what is usually referred to as molecular arms race. Components of the host’s immune system form a network that processes information about molecules with a foreign origin and damage-associated signals, integrating them with developmental and abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction networks have been extensively characterized. However, little is known on whether pathogenic effectors form complexes to overcome plant immunity and promote disease. Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces effectors that target hubs in the immune network of the host cell. Here we assess the capability of U. maydis effector candidates to interact with each other, which may play a crucial role during the infection process. Using a systematic yeast-two-hybrid approach and based on a preliminary pooled screen, we selected 63 putative effectors for one-on-one matings with a library of nearly 300 effector candidates. We found that 126 of these effector candidates interacted either with themselves or other predicted effectors. Although the functional relevance of the observed interactions remains elusive, we propose that the observed abundance in complex formation between effectors adds an additional level of complexity to effector research and should be taken into consideration when studying effector evolution and function. Based on this fundamental finding, we suggest various scenarios which could evolutionarily drive the formation and stabilization of an effector interactome."}],"publication_status":"published","publication":"Frontiers in Plant Science","language":[{"iso":"eng"}],"isi":1,"intvolume":"        10","has_accepted_license":"1","scopus_import":"1","oa":1,"date_updated":"2023-09-06T14:33:46Z","article_processing_charge":"No","issue":"11","title":"Systematic Y2H screening reveals extensive effector-complex formation","pmid":1,"status":"public","type":"journal_article","publication_identifier":{"eissn":["1664462X"]},"month":"11","day":"14","oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"date_created":"2019-12-16T07:58:43Z","date_updated":"2020-07-14T12:47:52Z","file_id":"7185","checksum":"995aa838aec2064d93550de82b40bbd1","file_name":"2019_FrontiersPlant_Alcantara.pdf","relation":"main_file","content_type":"application/pdf","file_size":1532505,"access_level":"open_access","creator":"dernst"}],"author":[{"last_name":"Alcântara","full_name":"Alcântara, André","first_name":"André"},{"full_name":"Bosch, Jason","first_name":"Jason","last_name":"Bosch"},{"first_name":"Fahimeh","full_name":"Nazari, Fahimeh","last_name":"Nazari"},{"last_name":"Hoffmann","first_name":"Gesa","full_name":"Hoffmann, Gesa"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368"},{"last_name":"Uhse","first_name":"Simon","full_name":"Uhse, Simon"},{"first_name":"Martin A.","full_name":"Darino, Martin A.","last_name":"Darino"},{"first_name":"Toluwase","full_name":"Olukayode, Toluwase","last_name":"Olukayode"},{"last_name":"Reumann","first_name":"Daniel","full_name":"Reumann, Daniel"},{"full_name":"Baggaley, Laura","first_name":"Laura","last_name":"Baggaley"},{"first_name":"Armin","full_name":"Djamei, Armin","last_name":"Djamei"}]},{"publication_status":"published","external_id":{"pmid":["30936248"],"isi":["000470086100045"]},"abstract":[{"text":"Polar auxin transport plays a pivotal role in plant growth and development. PIN auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.","lang":"eng"}],"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"publisher":"ASPB","date_published":"2019-06-01T00:00:00Z","page":"1152-1165","department":[{"_id":"JiFr"}],"quality_controlled":"1","volume":180,"acknowledgement":"We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.), and the European Union’s Horizon2020 program (European Research Council grant agreement no. 742985 to J.F.).","year":"2019","citation":{"apa":"Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., … Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. ASPB. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>","short":"A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi, T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K. Hayashi, Plant Physiology 180 (2019) 1152–1165.","ama":"Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. 2019;180(2):1152-1165. doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>","ieee":"A. Oochi <i>et al.</i>, “Pinstatic acid promotes auxin transport by inhibiting PIN internalization,” <i>Plant Physiology</i>, vol. 180, no. 2. ASPB, pp. 1152–1165, 2019.","ista":"Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology. 180(2), 1152–1165.","chicago":"Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy, K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>. ASPB, 2019. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>.","mla":"Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>, vol. 180, no. 2, ASPB, 2019, pp. 1152–65, doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>."},"date_created":"2019-04-09T08:38:20Z","ec_funded":1,"related_material":{"record":[{"status":"public","id":"11626","relation":"dissertation_contains"},{"relation":"dissertation_contains","id":"8822","status":"public"}]},"article_type":"original","_id":"6260","doi":"10.1104/pp.19.00201","author":[{"first_name":"A","full_name":"Oochi, A","last_name":"Oochi"},{"orcid":"0000-0003-2140-7195","last_name":"Hajny","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","first_name":"Jakub","full_name":"Hajny, Jakub"},{"full_name":"Fukui, K","first_name":"K","last_name":"Fukui"},{"last_name":"Nakao","first_name":"Y","full_name":"Nakao, Y"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei"},{"first_name":"M","full_name":"Quareshy, M","last_name":"Quareshy"},{"last_name":"Takahashi","full_name":"Takahashi, K","first_name":"K"},{"first_name":"T","full_name":"Kinoshita, T","last_name":"Kinoshita"},{"last_name":"Harborough","first_name":"SR","full_name":"Harborough, SR"},{"last_name":"Kepinski","first_name":"S","full_name":"Kepinski, S"},{"first_name":"H","full_name":"Kasahara, H","last_name":"Kasahara"},{"last_name":"Napier","full_name":"Napier, RM","first_name":"RM"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Hayashi","full_name":"Hayashi, KI","first_name":"KI"}],"month":"06","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1104/pp.19.00201"}],"oa_version":"Published Version","day":"01","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","pmid":1,"status":"public","type":"journal_article","oa":1,"date_updated":"2024-03-25T23:30:21Z","article_processing_charge":"No","issue":"2","title":"Pinstatic acid promotes auxin transport by inhibiting PIN internalization","isi":1,"scopus_import":"1","intvolume":"       180","language":[{"iso":"eng"}],"publication":"Plant Physiology"},{"has_accepted_license":"1","intvolume":"        19","scopus_import":"1","isi":1,"title":"The core effector Cce1 is required for early infection of maize by Ustilago maydis","oa":1,"date_updated":"2023-09-19T10:06:42Z","issue":"10","article_processing_charge":"No","publication":"Molecular Plant Pathology","language":[{"iso":"eng"}],"day":"01","oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"content_type":"application/pdf","file_size":682335,"relation":"main_file","access_level":"open_access","creator":"dernst","date_updated":"2018-12-18T09:46:00Z","date_created":"2018-12-18T09:46:00Z","file_id":"5740","file_name":"2018_MolecPlantPath_Seitner.pdf","success":1}],"month":"10","author":[{"first_name":"Denise","full_name":"Seitner, Denise","last_name":"Seitner"},{"last_name":"Uhse","first_name":"Simon","full_name":"Uhse, Simon"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Djamei","full_name":"Djamei, Armin","first_name":"Armin"}],"status":"public","type":"journal_article","citation":{"ieee":"D. Seitner, S. Uhse, M. C. Gallei, and A. Djamei, “The core effector Cce1 is required for early infection of maize by Ustilago maydis,” <i>Molecular Plant Pathology</i>, vol. 19, no. 10. Wiley, pp. 2277–2287, 2018.","ama":"Seitner D, Uhse S, Gallei MC, Djamei A. The core effector Cce1 is required for early infection of maize by Ustilago maydis. <i>Molecular Plant Pathology</i>. 2018;19(10):2277-2287. doi:<a href=\"https://doi.org/10.1111/mpp.12698\">10.1111/mpp.12698</a>","short":"D. Seitner, S. Uhse, M.C. Gallei, A. Djamei, Molecular Plant Pathology 19 (2018) 2277–2287.","mla":"Seitner, Denise, et al. “The Core Effector Cce1 Is Required for Early Infection of Maize by Ustilago Maydis.” <i>Molecular Plant Pathology</i>, vol. 19, no. 10, Wiley, 2018, pp. 2277–87, doi:<a href=\"https://doi.org/10.1111/mpp.12698\">10.1111/mpp.12698</a>.","ista":"Seitner D, Uhse S, Gallei MC, Djamei A. 2018. The core effector Cce1 is required for early infection of maize by Ustilago maydis. Molecular Plant Pathology. 19(10), 2277–2287.","chicago":"Seitner, Denise, Simon Uhse, Michelle C Gallei, and Armin Djamei. “The Core Effector Cce1 Is Required for Early Infection of Maize by Ustilago Maydis.” <i>Molecular Plant Pathology</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/mpp.12698\">https://doi.org/10.1111/mpp.12698</a>.","apa":"Seitner, D., Uhse, S., Gallei, M. C., &#38; Djamei, A. (2018). The core effector Cce1 is required for early infection of maize by Ustilago maydis. <i>Molecular Plant Pathology</i>. Wiley. <a href=\"https://doi.org/10.1111/mpp.12698\">https://doi.org/10.1111/mpp.12698</a>"},"publist_id":"7950","date_created":"2018-12-11T11:44:39Z","acknowledgement":"the Austrian Science Fund (FWF): [P27429‐B22, P27818‐B22, I 3033‐B22], and the Austrian Academy of Science (OEAW).","year":"2018","doi":"10.1111/mpp.12698","file_date_updated":"2018-12-18T09:46:00Z","_id":"104","external_id":{"isi":["000445624100006"]},"abstract":[{"lang":"eng","text":"The biotrophic pathogen Ustilago maydis, the causative agent of corn smut disease, infects one of the most important crops worldwide – Zea mays. To successfully colonize its host, U. maydis secretes proteins, known as effectors, that suppress plant defense responses and facilitate the establishment of biotrophy. In this work, we describe the U. maydis effector protein Cce1. Cce1 is essential for virulence and is upregulated during infection. Through microscopic analysis and in vitro assays, we show that Cce1 is secreted from hyphae during filamentous growth of the fungus. Strikingly, Δcce1 mutants are blocked at early stages of infection and induce callose deposition as a plant defense response. Cce1 is highly conserved among smut fungi and the Ustilago bromivora ortholog complemented the virulence defect of the SG200Δcce1 deletion strain. These data indicate that Cce1 is a core effector with apoplastic localization that is essential for U. maydis to infect its host."}],"ddc":["580"],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_status":"published","department":[{"_id":"GradSch"}],"page":"2277 - 2287","quality_controlled":"1","volume":19,"publisher":"Wiley","date_published":"2018-10-01T00:00:00Z"}]