---
_id: '1321'
abstract:
- lang: eng
text: Most migrating cells extrude their front by the force of actin polymerization.
Polymerization requires an initial nucleation step, which is mediated by factors
establishing either parallel filaments in the case of filopodia or branched filaments
that form the branched lamellipodial network. Branches are considered essential
for regular cell motility and are initiated by the Arp2/3 complex, which in turn
is activated by nucleation-promoting factors of the WASP and WAVE families. Here
we employed rapid amoeboid crawling leukocytes and found that deletion of the
WAVE complex eliminated actin branching and thus lamellipodia formation. The cells
were left with parallel filaments at the leading edge, which translated, depending
on the differentiation status of the cell, into a unipolar pointed cell shape
or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased
speed and enormous directional persistence, while they were unable to turn towards
chemotactic gradients. Cells with multiple filopodia retained chemotactic activity
but their migration was progressively impaired with increasing geometrical complexity
of the extracellular environment. These findings establish that diversified leading
edge protrusions serve as explorative structures while they slow down actual locomotion.
acknowledged_ssus:
- _id: SSU
acknowledgement: "This work was supported by the German Research Foundation (DFG)
Priority Program SP 1464 to T.E.B.S. and M.S., and European Research Council (ERC
GA 281556) and Human Frontiers Program grants to M.S.\r\nService Units of IST Austria
for excellent technical support."
article_processing_charge: No
article_type: original
author:
- first_name: Alexander F
full_name: Leithner, Alexander F
id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
last_name: Leithner
orcid: 0000-0002-1073-744X
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Jan
full_name: Müller, Jan
id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
last_name: Müller
- first_name: Anne
full_name: Reversat, Anne
id: 35B76592-F248-11E8-B48F-1D18A9856A87
last_name: Reversat
orcid: 0000-0003-0666-8928
- first_name: Markus
full_name: Brown, Markus
id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
last_name: Brown
- first_name: Jan
full_name: Schwarz, Jan
id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: David
full_name: De Gorter, David
last_name: De Gorter
- first_name: Florian
full_name: Schur, Florian
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
- first_name: Jonathan
full_name: Bayerl, Jonathan
last_name: Bayerl
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Frank
full_name: Lai, Frank
last_name: Lai
- first_name: Markus
full_name: Moser, Markus
last_name: Moser
- first_name: Dontscho
full_name: Kerjaschki, Dontscho
last_name: Kerjaschki
- first_name: Klemens
full_name: Rottner, Klemens
last_name: Rottner
- first_name: Victor
full_name: Small, Victor
last_name: Small
- first_name: Theresia
full_name: Stradal, Theresia
last_name: Stradal
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Leithner AF, Eichner A, Müller J, et al. Diversified actin protrusions promote
environmental exploration but are dispensable for locomotion of leukocytes. Nature
Cell Biology. 2016;18:1253-1259. doi:10.1038/ncb3426
apa: Leithner, A. F., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz,
J., … Sixt, M. K. (2016). Diversified actin protrusions promote environmental
exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology.
Nature Publishing Group. https://doi.org/10.1038/ncb3426
chicago: Leithner, Alexander F, Alexander Eichner, Jan Müller, Anne Reversat, Markus
Brown, Jan Schwarz, Jack Merrin, et al. “Diversified Actin Protrusions Promote
Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature
Cell Biology. Nature Publishing Group, 2016. https://doi.org/10.1038/ncb3426.
ieee: A. F. Leithner et al., “Diversified actin protrusions promote environmental
exploration but are dispensable for locomotion of leukocytes,” Nature Cell
Biology, vol. 18. Nature Publishing Group, pp. 1253–1259, 2016.
ista: Leithner AF, Eichner A, Müller J, Reversat A, Brown M, Schwarz J, Merrin J,
De Gorter D, Schur FK, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai F, Moser
M, Kerjaschki D, Rottner K, Small V, Stradal T, Sixt MK. 2016. Diversified actin
protrusions promote environmental exploration but are dispensable for locomotion
of leukocytes. Nature Cell Biology. 18, 1253–1259.
mla: Leithner, Alexander F., et al. “Diversified Actin Protrusions Promote Environmental
Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature Cell
Biology, vol. 18, Nature Publishing Group, 2016, pp. 1253–59, doi:10.1038/ncb3426.
short: A.F. Leithner, A. Eichner, J. Müller, A. Reversat, M. Brown, J. Schwarz,
J. Merrin, D. De Gorter, F.K. Schur, J. Bayerl, I. de Vries, S. Wieser, R. Hauschild,
F. Lai, M. Moser, D. Kerjaschki, K. Rottner, V. Small, T. Stradal, M.K. Sixt,
Nature Cell Biology 18 (2016) 1253–1259.
date_created: 2018-12-11T11:51:21Z
date_published: 2016-10-24T00:00:00Z
date_updated: 2024-03-25T23:30:09Z
day: '24'
ddc:
- '570'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/ncb3426
ec_funded: 1
file:
- access_level: open_access
checksum: e1411cb7c99a2d9089c178a6abef25e7
content_type: application/pdf
creator: dernst
date_created: 2020-05-14T16:33:46Z
date_updated: 2020-07-14T12:44:43Z
file_id: '7844'
file_name: 2018_NatureCell_Leithner.pdf
file_size: 4433280
relation: main_file
file_date_updated: 2020-07-14T12:44:43Z
has_accepted_license: '1'
intvolume: ' 18'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '10'
oa: 1
oa_version: Submitted Version
page: 1253 - 1259
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5949'
quality_controlled: '1'
related_material:
record:
- id: '323'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Diversified actin protrusions promote environmental exploration but are dispensable
for locomotion of leukocytes
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2016'
...
---
_id: '1239'
abstract:
- lang: eng
text: Nonadherent polarized cells have been observed to have a pearlike, elongated
shape. Using a minimal model that describes the cell cortex as a thin layer of
contractile active gel, we show that the anisotropy of active stresses, controlled
by cortical viscosity and filament ordering, can account for this morphology.
The predicted shapes can be determined from the flow pattern only; they prove
to be independent of the mechanism at the origin of the cortical flow, and are
only weakly sensitive to the cytoplasmic rheology. In the case of actin flows
resulting from a contractile instability, we propose a phase diagram of three-dimensional
cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate
shapes, all of which have been observed in experiment.
acknowledgement: 'V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant
No. T560-B17).'
article_number: '028102'
author:
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical
flow-driven shapes of nonadherent cells. Physical Review Letters. 2016;116(2).
doi:10.1103/PhysRevLett.116.028102
apa: Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez,
R. (2016). Cortical flow-driven shapes of nonadherent cells. Physical Review
Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.116.028102
chicago: Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg,
and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical
Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.116.028102.
ieee: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez,
“Cortical flow-driven shapes of nonadherent cells,” Physical Review Letters,
vol. 116, no. 2. American Physical Society, 2016.
ista: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016.
Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2),
028102.
mla: Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.”
Physical Review Letters, vol. 116, no. 2, 028102, American Physical Society,
2016, doi:10.1103/PhysRevLett.116.028102.
short: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez,
Physical Review Letters 116 (2016).
date_created: 2018-12-11T11:50:53Z
date_published: 2016-01-15T00:00:00Z
date_updated: 2021-01-12T06:49:19Z
day: '15'
department:
- _id: CaHe
doi: 10.1103/PhysRevLett.116.028102
intvolume: ' 116'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
publication: Physical Review Letters
publication_status: published
publisher: American Physical Society
publist_id: '6095'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cortical flow-driven shapes of nonadherent cells
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 116
year: '2016'
...
---
_id: '1275'
article_number: '139802'
author:
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones
et al. Reply. Physical Review Letters. 2016;117(13). doi:10.1103/PhysRevLett.117.139802
apa: Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez,
R. (2016). Callan-Jones et al. Reply. Physical Review Letters. American
Physical Society. https://doi.org/10.1103/PhysRevLett.117.139802
chicago: Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg,
and Raphaël Voituriez. “Callan-Jones et Al. Reply.” Physical Review Letters.
American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.117.139802.
ieee: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez,
“Callan-Jones et al. Reply,” Physical Review Letters, vol. 117, no. 13.
American Physical Society, 2016.
ista: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016.
Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.
mla: Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” Physical Review
Letters, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:10.1103/PhysRevLett.117.139802.
short: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez,
Physical Review Letters 117 (2016).
date_created: 2018-12-11T11:51:05Z
date_published: 2016-09-22T00:00:00Z
date_updated: 2021-01-12T06:49:33Z
day: '22'
department:
- _id: CaHe
doi: 10.1103/PhysRevLett.117.139802
intvolume: ' 117'
issue: '13'
language:
- iso: eng
month: '09'
oa_version: None
publication: Physical Review Letters
publication_status: published
publisher: American Physical Society
publist_id: '6041'
quality_controlled: '1'
scopus_import: 1
status: public
title: Callan-Jones et al. Reply
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 117
year: '2016'
...
---
_id: '1537'
abstract:
- lang: eng
text: 3D amoeboid cell migration is central to many developmental and disease-related
processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid
cell migration mode in early zebrafish embryos, termed stable-bleb migration.
Stable-bleb cells display an invariant polarized balloon-like shape with exceptional
migration speed and persistence. Progenitor cells can be reversibly transformed
into stable-bleb cells irrespective of their primary fate and motile characteristics
by increasing myosin II activity through biochemical or mechanical stimuli. Using
a combination of theory and experiments, we show that, in stable-bleb cells, cortical
contractility fluctuations trigger a stochastic switch into amoeboid motility,
and a positive feedback between cortical flows and gradients in contractility
maintains stable-bleb cell polarization. We further show that rearward cortical
flows drive stable-bleb cell migration in various adhesive and non-adhesive environments,
unraveling a highly versatile amoeboid migration phenotype.
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We would like to thank R. Hausschild and E. Papusheva for technical
assistance and the service facilities at the IST Austria for continuous support.
The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel
and E. Paluch for exchanging unpublished data. '
author:
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Michael
full_name: Smutny, Michael
id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
last_name: Smutny
orcid: 0000-0002-5920-9090
- first_name: Hitoshi
full_name: Morita, Hitoshi
id: 4C6E54C6-F248-11E8-B48F-1D18A9856A87
last_name: Morita
- first_name: Keisuke
full_name: Sako, Keisuke
id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
last_name: Sako
orcid: 0000-0002-6453-8075
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Monika
full_name: Ritsch Marte, Monika
last_name: Ritsch Marte
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers
a stochastic switch to fast amoeboid cell motility. Cell. 2015;160(4):673-685.
doi:10.1016/j.cell.2015.01.008
apa: Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K.,
… Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch
to fast amoeboid cell motility. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.008
chicago: Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi
Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers
a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell. Cell Press,
2015. https://doi.org/10.1016/j.cell.2015.01.008.
ieee: V. Ruprecht et al., “Cortical contractility triggers a stochastic switch
to fast amoeboid cell motility,” Cell, vol. 160, no. 4. Cell Press, pp.
673–685, 2015.
ista: Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V,
Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility
triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.
mla: Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch
to Fast Amoeboid Cell Motility.” Cell, vol. 160, no. 4, Cell Press, 2015,
pp. 673–85, doi:10.1016/j.cell.2015.01.008.
short: V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V.
Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160
(2015) 673–685.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-02-12T00:00:00Z
date_updated: 2023-09-07T12:05:08Z
day: '12'
ddc:
- '570'
department:
- _id: CaHe
- _id: MiSi
doi: 10.1016/j.cell.2015.01.008
file:
- access_level: open_access
checksum: 228d3edf40627d897b3875088a0ac51f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:21Z
date_updated: 2020-07-14T12:45:01Z
file_id: '5003'
file_name: IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf
file_size: 4362653
relation: main_file
file_date_updated: 2020-07-14T12:45:01Z
has_accepted_license: '1'
intvolume: ' 160'
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 673 - 685
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 812-B12
name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5634'
pubrep_id: '484'
quality_controlled: '1'
related_material:
record:
- id: '961'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Cortical contractility triggers a stochastic switch to fast amoeboid cell motility
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 160
year: '2015'
...
---
_id: '1553'
abstract:
- lang: eng
text: Cell movement has essential functions in development, immunity, and cancer.
Various cell migration patterns have been reported, but no general rule has emerged
so far. Here, we show on the basis of experimental data in vitro and in vivo that
cell persistence, which quantifies the straightness of trajectories, is robustly
coupled to cell migration speed. We suggest that this universal coupling constitutes
a generic law of cell migration, which originates in the advection of polarity
cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis
relies on a theoretical model that we validate by measuring the persistence of
cells upon modulation of actin flow speeds and upon optogenetic manipulation of
the binding of an actin regulator to actin filaments. Beyond the quantitative
prediction of the coupling, the model yields a generic phase diagram of cellular
trajectories, which recapitulates the full range of observed migration patterns.
author:
- first_name: Paolo
full_name: Maiuri, Paolo
last_name: Maiuri
- first_name: Jean
full_name: Rupprecht, Jean
last_name: Rupprecht
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Olivier
full_name: Bénichou, Olivier
last_name: Bénichou
- first_name: Nicolas
full_name: Carpi, Nicolas
last_name: Carpi
- first_name: Mathieu
full_name: Coppey, Mathieu
last_name: Coppey
- first_name: Simon
full_name: De Beco, Simon
last_name: De Beco
- first_name: Nir
full_name: Gov, Nir
last_name: Gov
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Carolina
full_name: Lage Crespo, Carolina
last_name: Lage Crespo
- first_name: Franziska
full_name: Lautenschlaeger, Franziska
last_name: Lautenschlaeger
- first_name: Maël
full_name: Le Berre, Maël
last_name: Le Berre
- first_name: Ana
full_name: Lennon Duménil, Ana
last_name: Lennon Duménil
- first_name: Matthew
full_name: Raab, Matthew
last_name: Raab
- first_name: Hawa
full_name: Thiam, Hawa
last_name: Thiam
- first_name: Matthieu
full_name: Piel, Matthieu
last_name: Piel
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling
between cell speed and cell persistence. Cell. 2015;161(2):374-386. doi:10.1016/j.cell.2015.01.056
apa: Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N.,
… Voituriez, R. (2015). Actin flows mediate a universal coupling between cell
speed and cell persistence. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.056
chicago: Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier
Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal
Coupling between Cell Speed and Cell Persistence.” Cell. Cell Press, 2015.
https://doi.org/10.1016/j.cell.2015.01.056.
ieee: P. Maiuri et al., “Actin flows mediate a universal coupling between
cell speed and cell persistence,” Cell, vol. 161, no. 2. Cell Press, pp.
374–386, 2015.
ista: Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M,
De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre
M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin
flows mediate a universal coupling between cell speed and cell persistence. Cell.
161(2), 374–386.
mla: Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell
Speed and Cell Persistence.” Cell, vol. 161, no. 2, Cell Press, 2015, pp.
374–86, doi:10.1016/j.cell.2015.01.056.
short: P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M.
Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger,
M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez,
Cell 161 (2015) 374–386.
date_created: 2018-12-11T11:52:41Z
date_published: 2015-04-09T00:00:00Z
date_updated: 2021-01-12T06:51:33Z
day: '09'
department:
- _id: MiSi
- _id: CaHe
doi: 10.1016/j.cell.2015.01.056
ec_funded: 1
intvolume: ' 161'
issue: '2'
language:
- iso: eng
month: '04'
oa_version: None
page: 374 - 386
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
- _id: 25ABD200-B435-11E9-9278-68D0E5697425
grant_number: RGP0058/2011
name: 'Cell migration in complex environments: from in vivo experiments to theoretical
models'
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5618'
quality_controlled: '1'
scopus_import: 1
status: public
title: Actin flows mediate a universal coupling between cell speed and cell persistence
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 161
year: '2015'
...
---
_id: '1925'
abstract:
- lang: eng
text: In the past decade carbon nanotubes (CNTs) have been widely studied as a potential
drug-delivery system, especially with functionality for cellular targeting. Yet,
little is known about the actual process of docking to cell receptors and transport
dynamics after internalization. Here we performed single-particle studies of folic
acid (FA) mediated CNT binding to human carcinoma cells and their transport inside
the cytosol. In particular, we employed molecular recognition force spectroscopy,
an atomic force microscopy based method, to visualize and quantify docking of
FA functionalized CNTs to FA binding receptors in terms of binding probability
and binding force. We then traced individual fluorescently labeled, FA functionalized
CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed
trajectories of directed diffusion and areas of nanotube confinement in the cytosol.
Our results demonstrate the potential of a single-molecule approach for investigation
of drug-delivery vehicles and their targeting capacity.
acknowledgement: "This work was supported by EC grant Marie Curie RTN-CT-2006-035616,
CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net
823980, 'IntelliTip'.\r\n"
article_number: '125704'
article_processing_charge: No
article_type: original
author:
- first_name: Constanze
full_name: Lamprecht, Constanze
last_name: Lamprecht
- first_name: Birgit
full_name: Plochberger, Birgit
last_name: Plochberger
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Christian
full_name: Rankl, Christian
last_name: Rankl
- first_name: Elena
full_name: Heister, Elena
last_name: Heister
- first_name: Barbara
full_name: Unterauer, Barbara
last_name: Unterauer
- first_name: Mario
full_name: Brameshuber, Mario
last_name: Brameshuber
- first_name: Jürgen
full_name: Danzberger, Jürgen
last_name: Danzberger
- first_name: Petar
full_name: Lukanov, Petar
last_name: Lukanov
- first_name: Emmanuel
full_name: Flahaut, Emmanuel
last_name: Flahaut
- first_name: Gerhard
full_name: Schütz, Gerhard
last_name: Schütz
- first_name: Peter
full_name: Hinterdorfer, Peter
last_name: Hinterdorfer
- first_name: Andreas
full_name: Ebner, Andreas
last_name: Ebner
citation:
ama: Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to
explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology.
2014;25(12). doi:10.1088/0957-4484/25/12/125704
apa: Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister,
E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and
transport of cancer cell targeting nanotubes. Nanotechnology. IOP Publishing.
https://doi.org/10.1088/0957-4484/25/12/125704
chicago: Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser,
Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach
to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” Nanotechnology.
IOP Publishing, 2014. https://doi.org/10.1088/0957-4484/25/12/125704.
ieee: C. Lamprecht et al., “A single-molecule approach to explore binding
uptake and transport of cancer cell targeting nanotubes,” Nanotechnology,
vol. 25, no. 12. IOP Publishing, 2014.
ista: Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer
B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P,
Ebner A. 2014. A single-molecule approach to explore binding uptake and transport
of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.
mla: Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding
Uptake and Transport of Cancer Cell Targeting Nanotubes.” Nanotechnology,
vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:10.1088/0957-4484/25/12/125704.
short: C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister,
B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz,
P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).
date_created: 2018-12-11T11:54:45Z
date_published: 2014-03-28T00:00:00Z
date_updated: 2021-01-12T06:54:07Z
day: '28'
ddc:
- '570'
department:
- _id: CaHe
- _id: MiSi
doi: 10.1088/0957-4484/25/12/125704
file:
- access_level: open_access
checksum: df4e03d225a19179e7790f6d87a12332
content_type: application/pdf
creator: dernst
date_created: 2020-05-15T09:21:19Z
date_updated: 2020-07-14T12:45:21Z
file_id: '7856'
file_name: 2014_Nanotechnology_Lamprecht.pdf
file_size: 3804152
relation: main_file
file_date_updated: 2020-07-14T12:45:21Z
has_accepted_license: '1'
intvolume: ' 25'
issue: '12'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
publication: Nanotechnology
publication_status: published
publisher: IOP Publishing
publist_id: '5169'
scopus_import: 1
status: public
title: A single-molecule approach to explore binding uptake and transport of cancer
cell targeting nanotubes
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2014'
...
---
_id: '3285'
abstract:
- lang: eng
text: Resolving the dynamical interplay of proteins and lipids in the live-cell
plasma membrane represents a central goal in current cell biology. Superresolution
concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic
length scale. Similar concepts for detecting dynamical transitions (superresolution
chronoscopy) are still lacking. Here, we show that recently introduced spot-variation
fluorescence correlation spectroscopy allows for sensing transient confinement
times of membrane constituents at dramatically improved resolution. Using standard
diffraction-limited optics, spot-variation fluorescence correlation spectroscopy
captures signatures of single retardation events far below the transit time of
the tracer through the focal spot. We provide an analytical description of special
cases of transient binding of a tracer to pointlike traps, or association of a
tracer with nanodomains. The influence of trap mobility and the underlying binding
kinetics are quantified. Experimental approaches are suggested that allow for
gaining quantitative mechanistic insights into the interaction processes of membrane
constituents.
acknowledgement: Y 250-B03/Austrian Science Fund FWF/Austria
author:
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Didier
full_name: Marguet, Didier
last_name: Marguet
- first_name: Gerhard
full_name: Schuetz, Gerhard
last_name: Schuetz
citation:
ama: Ruprecht V, Wieser S, Marguet D, Schuetz G. Spot variation fluorescence correlation
spectroscopy allows for superresolution chronoscopy of confinement times in membranes.
Biophysical Journal. 2011;100(11):2839-2845. doi:10.1016/j.bpj.2011.04.035
apa: Ruprecht, V., Wieser, S., Marguet, D., & Schuetz, G. (2011). Spot variation
fluorescence correlation spectroscopy allows for superresolution chronoscopy of
confinement times in membranes. Biophysical Journal. Biophysical Society.
https://doi.org/10.1016/j.bpj.2011.04.035
chicago: Ruprecht, Verena, Stefan Wieser, Didier Marguet, and Gerhard Schuetz. “Spot
Variation Fluorescence Correlation Spectroscopy Allows for Superresolution Chronoscopy
of Confinement Times in Membranes.” Biophysical Journal. Biophysical Society,
2011. https://doi.org/10.1016/j.bpj.2011.04.035.
ieee: V. Ruprecht, S. Wieser, D. Marguet, and G. Schuetz, “Spot variation fluorescence
correlation spectroscopy allows for superresolution chronoscopy of confinement
times in membranes,” Biophysical Journal, vol. 100, no. 11. Biophysical
Society, pp. 2839–2845, 2011.
ista: Ruprecht V, Wieser S, Marguet D, Schuetz G. 2011. Spot variation fluorescence
correlation spectroscopy allows for superresolution chronoscopy of confinement
times in membranes. Biophysical Journal. 100(11), 2839–2845.
mla: Ruprecht, Verena, et al. “Spot Variation Fluorescence Correlation Spectroscopy
Allows for Superresolution Chronoscopy of Confinement Times in Membranes.” Biophysical
Journal, vol. 100, no. 11, Biophysical Society, 2011, pp. 2839–45, doi:10.1016/j.bpj.2011.04.035.
short: V. Ruprecht, S. Wieser, D. Marguet, G. Schuetz, Biophysical Journal 100 (2011)
2839–2845.
date_created: 2018-12-11T12:02:27Z
date_published: 2011-06-08T00:00:00Z
date_updated: 2021-01-12T07:42:23Z
day: '08'
doi: 10.1016/j.bpj.2011.04.035
extern: '1'
intvolume: ' 100'
issue: '11'
language:
- iso: eng
month: '06'
oa_version: None
page: 2839 - 2845
publication: Biophysical Journal
publication_status: published
publisher: Biophysical Society
publist_id: '3360'
status: public
title: Spot variation fluorescence correlation spectroscopy allows for superresolution
chronoscopy of confinement times in membranes
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 100
year: '2011'
...
---
_id: '3286'
abstract:
- lang: eng
text: Cationic antimicrobial peptides (CAMPs) selectively target bacterial membranes
by electrostatic interactions with negatively charged lipids. It turned out that
for inhibition of microbial growth a high CAMP membrane concentration is required,
which can be realized by the incorporation of hydrophobic groups within the peptide.
Increasing hydrophobicity, however, reduces the CAMP selectivity for bacterial
over eukaryotic host membranes, thereby causing the risk of detrimental side-effects.
In this study we addressed how cationic amphipathic peptides—in particular a CAMP
with Lysine–Leucine–Lysine repeats (termed KLK)—affect the localization and dynamics
of molecules in eukaryotic membranes. We found KLK to selectively inhibit the
endocytosis of a subgroup of membrane proteins and lipids by electrostatically
interacting with negatively charged sialic acid moieties. Ultrastructural characterization
revealed the formation of membrane invaginations representing fission or fusion
intermediates, in which the sialylated proteins and lipids were immobilized. Experiments
on structurally different cationic amphipathic peptides (KLK, 6-MO-LF11-322 and
NK14-2) indicated a cooperation of electrostatic and hydrophobic forces that selectively
arrest sialylated membrane constituents.
acknowledgement: "This work was funded by the GEN-AU project of the Austrian Research
Promotion Agency, the Austrian Science Fund (FWF; project Y250-B03) and Intercell
AG.\nWe thank the following colleagues for providing plasmids and cells: Daniel
Legler (University of Konstanz, Switzerland), Jennifer Lippincott-Schwartz (NIH,
Bethesda, USA), Hannes Stockinger (Medical University Vienna, Austria), Katharina
Strub (University of Geneva, Switzerland), Lawrence Rajendran (ETH Zurich, Switzerland),
Eileen M. Lafer (UTHSC San Antonio, Texas, USA), Mark McNiven (Mayo Clinic, Minnesota,
USA), John Silvius (McGill University, Montreal, Canada), Christoph Romanin (JKU
Linz, Austria), Herbert Stangl (Medical University Vienna, Austria) and Anton van
der Merwe (Oxford University, Oxford, UK). We thank Harald Kotisch (MFPL, Vienna)
for excellent technical assistance in the processing of samples for electron microscopy
and Sergio Grinstein (Hospital for Sick Children Research Institute, Toronto) for
fruitful discussions. "
author:
- first_name: Julian
full_name: Weghuber, Julian
last_name: Weghuber
- first_name: Michael
full_name: Aichinger, Michael C.
last_name: Aichinger
- first_name: Mario
full_name: Brameshuber, Mario
last_name: Brameshuber
- first_name: Stefan
full_name: Stefan Wieser
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Verena
full_name: Verena Ruprecht
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Birgit
full_name: Plochberger, Birgit
last_name: Plochberger
- first_name: Josef
full_name: Madl, Josef
last_name: Madl
- first_name: Andreas
full_name: Horner, Andreas
last_name: Horner
- first_name: Siegfried
full_name: Reipert, Siegfried
last_name: Reipert
- first_name: Karl
full_name: Lohner, Karl
last_name: Lohner
- first_name: Tamas
full_name: Henics, Tamas
last_name: Henics
- first_name: Gerhard
full_name: Schuetz, Gerhard J
last_name: Schuetz
citation:
ama: Weghuber J, Aichinger M, Brameshuber M, et al. Cationic amphipathic peptides
accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic
host cells. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2011;1808(10):2581-2590.
doi:10.1016/j.bbamem.2011.06.007
apa: Weghuber, J., Aichinger, M., Brameshuber, M., Wieser, S., Ruprecht, V., Plochberger,
B., … Schuetz, G. (2011). Cationic amphipathic peptides accumulate sialylated
proteins and lipids in the plasma membrane of eukaryotic host cells. Biochimica
et Biophysica Acta (BBA) - Biomembranes. Elsevier. https://doi.org/10.1016/j.bbamem.2011.06.007
chicago: Weghuber, Julian, Michael Aichinger, Mario Brameshuber, Stefan Wieser,
Verena Ruprecht, Birgit Plochberger, Josef Madl, et al. “Cationic Amphipathic
Peptides Accumulate Sialylated Proteins and Lipids in the Plasma Membrane of Eukaryotic
Host Cells.” Biochimica et Biophysica Acta (BBA) - Biomembranes. Elsevier,
2011. https://doi.org/10.1016/j.bbamem.2011.06.007.
ieee: J. Weghuber et al., “Cationic amphipathic peptides accumulate sialylated
proteins and lipids in the plasma membrane of eukaryotic host cells,” Biochimica
et Biophysica Acta (BBA) - Biomembranes, vol. 1808, no. 10. Elsevier, pp.
2581–2590, 2011.
ista: Weghuber J, Aichinger M, Brameshuber M, Wieser S, Ruprecht V, Plochberger
B, Madl J, Horner A, Reipert S, Lohner K, Henics T, Schuetz G. 2011. Cationic
amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane
of eukaryotic host cells. Biochimica et Biophysica Acta (BBA) - Biomembranes.
1808(10), 2581–2590.
mla: Weghuber, Julian, et al. “Cationic Amphipathic Peptides Accumulate Sialylated
Proteins and Lipids in the Plasma Membrane of Eukaryotic Host Cells.” Biochimica
et Biophysica Acta (BBA) - Biomembranes, vol. 1808, no. 10, Elsevier, 2011,
pp. 2581–90, doi:10.1016/j.bbamem.2011.06.007.
short: J. Weghuber, M. Aichinger, M. Brameshuber, S. Wieser, V. Ruprecht, B. Plochberger,
J. Madl, A. Horner, S. Reipert, K. Lohner, T. Henics, G. Schuetz, Biochimica et
Biophysica Acta (BBA) - Biomembranes 1808 (2011) 2581–2590.
date_created: 2018-12-11T12:02:28Z
date_published: 2011-10-01T00:00:00Z
date_updated: 2021-01-12T07:42:24Z
day: '01'
doi: 10.1016/j.bbamem.2011.06.007
extern: 1
intvolume: ' 1808'
issue: '10'
month: '10'
page: 2581 - 2590
publication: Biochimica et Biophysica Acta (BBA) - Biomembranes
publication_status: published
publisher: Elsevier
publist_id: '3359'
quality_controlled: 0
status: public
title: Cationic amphipathic peptides accumulate sialylated proteins and lipids in
the plasma membrane of eukaryotic host cells
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
volume: 1808
year: '2011'
...
---
_id: '3287'
abstract:
- lang: eng
text: 'Diffusing membrane constituents are constantly exposed to a variety of forces
that influence their stochastic path. Single molecule experiments allow for resolving
trajectories at extremely high spatial and temporal accuracy, thereby offering
insights into en route interactions of the tracer. In this review we discuss approaches
to derive information about the underlying processes, based on single molecule
tracking experiments. In particular, we focus on a new versatile way to analyze
single molecule diffusion in the absence of a full analytical treatment. The method
is based on comprehensive comparison of an experimental data set against the hypothetical
outcome of multiple experiments performed on the computer. Since Monte Carlo simulations
can be easily and rapidly performed even on state-of-the-art PCs, our method provides
a simple way for testing various - even complicated - diffusion models. We describe
the new method in detail, and show the applicability on two specific examples:
firstly, kinetic rate constants can be derived for the transient interaction of
mobile membrane proteins; secondly, residence time and corral size can be extracted
for confined diffusion.'
author:
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Markus
full_name: Axmann, Markus
last_name: Axmann
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Gerhard
full_name: Schuetz, Gerhard
last_name: Schuetz
citation:
ama: Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule
trajectories? Current Protein & Peptide Science. 2011;12(8):714-724.
doi:10.2174/138920311798841753
apa: Ruprecht, V., Axmann, M., Wieser, S., & Schuetz, G. (2011). What can we
learn from single molecule trajectories? Current Protein & Peptide Science.
Bentham Science Publishers. https://doi.org/10.2174/138920311798841753
chicago: Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What
Can We Learn from Single Molecule Trajectories?” Current Protein & Peptide
Science. Bentham Science Publishers, 2011. https://doi.org/10.2174/138920311798841753.
ieee: V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from
single molecule trajectories?,” Current Protein & Peptide Science,
vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.
ista: Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single
molecule trajectories? Current Protein & Peptide Science. 12(8), 714–724.
mla: Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?”
Current Protein & Peptide Science, vol. 12, no. 8, Bentham Science
Publishers, 2011, pp. 714–24, doi:10.2174/138920311798841753.
short: V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein & Peptide
Science 12 (2011) 714–724.
date_created: 2018-12-11T12:02:28Z
date_published: 2011-12-01T00:00:00Z
date_updated: 2021-01-12T07:42:24Z
day: '01'
department:
- _id: CaHe
- _id: MiSi
doi: 10.2174/138920311798841753
intvolume: ' 12'
issue: '8'
language:
- iso: eng
month: '12'
oa_version: None
page: 714 - 724
publication: Current Protein & Peptide Science
publication_status: published
publisher: Bentham Science Publishers
publist_id: '3358'
quality_controlled: '1'
scopus_import: 1
status: public
title: What can we learn from single molecule trajectories?
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2011'
...