---
_id: '14781'
abstract:
- lang: eng
  text: Germ granules, condensates of phase-separated RNA and protein, are organelles
    that are essential for germline development in different organisms. The patterning
    of the granules and their relevance for germ cell fate are not fully understood.
    Combining three-dimensional in vivo structural and functional analyses, we study
    the dynamic spatial organization of molecules within zebrafish germ granules.
    We find that the localization of RNA molecules to the periphery of the granules,
    where ribosomes are localized, depends on translational activity at this location.
    In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential
    for nanos3 RNA localization at the condensates’ periphery. Accordingly, in the
    absence of Dnd1, or when translation is inhibited, nanos3 RNA translocates into
    the granule interior, away from the ribosomes, a process that is correlated with
    the loss of germ cell fate. These findings highlight the relevance of sub-granule
    compartmentalization for post-transcriptional control and its importance for preserving
    germ cell totipotency.
acknowledgement: We thank Celeste Brennecka for editing and Michal Reichman-Fried
  for critical comments on the manuscript. We thank Ursula Jordan, Esther Messerschmidt,
  and Ines Sandbote for technical assistance. This work was supported by funding from
  the University of Münster (K.J.W., K.T., E.R., A.G., T.G.-T., J.S., and M.G.), the
  Max Planck Institute for Molecular Biomedicine (D.Z.), the German Research Foundation
  grant CRU 326 (P2) RA863/12-2 (E.R.), Baylor University (K.H. and D.R.), and the
  National Institutes of Health grant R35 GM 134910 (D.R.). We thank the referees
  for insightful comments that helped improve the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Kim Joana
  full_name: Westerich, Kim Joana
  last_name: Westerich
- first_name: Katsiaryna
  full_name: Tarbashevich, Katsiaryna
  last_name: Tarbashevich
- first_name: Jan
  full_name: Schick, Jan
  last_name: Schick
- first_name: Antra
  full_name: Gupta, Antra
  last_name: Gupta
- first_name: Mingzhao
  full_name: Zhu, Mingzhao
  last_name: Zhu
- first_name: Kenneth
  full_name: Hull, Kenneth
  last_name: Hull
- first_name: Daniel
  full_name: Romo, Daniel
  last_name: Romo
- first_name: Dagmar
  full_name: Zeuschner, Dagmar
  last_name: Zeuschner
- first_name: Mohammad
  full_name: Goudarzi, Mohammad
  id: 3384113A-F248-11E8-B48F-1D18A9856A87
  last_name: Goudarzi
- first_name: Theresa
  full_name: Gross-Thebing, Theresa
  last_name: Gross-Thebing
- first_name: Erez
  full_name: Raz, Erez
  last_name: Raz
citation:
  ama: Westerich KJ, Tarbashevich K, Schick J, et al. Spatial organization and function
    of RNA molecules within phase-separated condensates in zebrafish are controlled
    by Dnd1. <i>Developmental Cell</i>. 2023;58(17):1578-1592.e5. doi:<a href="https://doi.org/10.1016/j.devcel.2023.06.009">10.1016/j.devcel.2023.06.009</a>
  apa: Westerich, K. J., Tarbashevich, K., Schick, J., Gupta, A., Zhu, M., Hull, K.,
    … Raz, E. (2023). Spatial organization and function of RNA molecules within phase-separated
    condensates in zebrafish are controlled by Dnd1. <i>Developmental Cell</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.devcel.2023.06.009">https://doi.org/10.1016/j.devcel.2023.06.009</a>
  chicago: Westerich, Kim Joana, Katsiaryna Tarbashevich, Jan Schick, Antra Gupta,
    Mingzhao Zhu, Kenneth Hull, Daniel Romo, et al. “Spatial Organization and Function
    of RNA Molecules within Phase-Separated Condensates in Zebrafish Are Controlled
    by Dnd1.” <i>Developmental Cell</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.devcel.2023.06.009">https://doi.org/10.1016/j.devcel.2023.06.009</a>.
  ieee: K. J. Westerich <i>et al.</i>, “Spatial organization and function of RNA molecules
    within phase-separated condensates in zebrafish are controlled by Dnd1,” <i>Developmental
    Cell</i>, vol. 58, no. 17. Elsevier, p. 1578–1592.e5, 2023.
  ista: Westerich KJ, Tarbashevich K, Schick J, Gupta A, Zhu M, Hull K, Romo D, Zeuschner
    D, Goudarzi M, Gross-Thebing T, Raz E. 2023. Spatial organization and function
    of RNA molecules within phase-separated condensates in zebrafish are controlled
    by Dnd1. Developmental Cell. 58(17), 1578–1592.e5.
  mla: Westerich, Kim Joana, et al. “Spatial Organization and Function of RNA Molecules
    within Phase-Separated Condensates in Zebrafish Are Controlled by Dnd1.” <i>Developmental
    Cell</i>, vol. 58, no. 17, Elsevier, 2023, p. 1578–1592.e5, doi:<a href="https://doi.org/10.1016/j.devcel.2023.06.009">10.1016/j.devcel.2023.06.009</a>.
  short: K.J. Westerich, K. Tarbashevich, J. Schick, A. Gupta, M. Zhu, K. Hull, D.
    Romo, D. Zeuschner, M. Goudarzi, T. Gross-Thebing, E. Raz, Developmental Cell
    58 (2023) 1578–1592.e5.
date_created: 2024-01-10T09:41:21Z
date_published: 2023-09-11T00:00:00Z
date_updated: 2024-01-16T08:56:36Z
day: '11'
department:
- _id: Bio
doi: 10.1016/j.devcel.2023.06.009
external_id:
  pmid:
  - '37463577'
intvolume: '        58'
issue: '17'
keyword:
- Developmental Biology
- Cell Biology
- General Biochemistry
- Genetics and Molecular Biology
- Molecular Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/2023.07.09.548244
month: '09'
oa: 1
oa_version: Preprint
page: 1578-1592.e5
pmid: 1
publication: Developmental Cell
publication_identifier:
  issn:
  - 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Spatial organization and function of RNA molecules within phase-separated condensates
  in zebrafish are controlled by Dnd1
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 58
year: '2023'
...
---
_id: '6093'
abstract:
- lang: eng
  text: Blebs are cellular protrusions observed in migrating cells and in cells undergoing
    spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm
    during bleb formation and the concurrent changes in cell volume using zebrafish
    primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation
    occurs concomitantly with cytoplasmic inflow into it and that during this process
    the total cell volume does not change. We thus show that bleb formation in primordial
    germ cells results primarily from redistribution of material within the cell rather
    than being driven by flow of water from an external source.
article_number: e0212699
article_processing_charge: No
author:
- first_name: Mohammad
  full_name: Goudarzi, Mohammad
  id: 3384113A-F248-11E8-B48F-1D18A9856A87
  last_name: Goudarzi
- first_name: Aleix
  full_name: Boquet-Pujadas, Aleix
  last_name: Boquet-Pujadas
- first_name: Jean Christophe
  full_name: Olivo-Marin, Jean Christophe
  last_name: Olivo-Marin
- first_name: Erez
  full_name: Raz, Erez
  last_name: Raz
citation:
  ama: Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. Fluid dynamics during
    bleb formation in migrating cells in vivo. <i>PLOS ONE</i>. 2019;14(2). doi:<a
    href="https://doi.org/10.1371/journal.pone.0212699">10.1371/journal.pone.0212699</a>
  apa: Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J. C., &#38; Raz, E. (2019).
    Fluid dynamics during bleb formation in migrating cells in vivo. <i>PLOS ONE</i>.
    Public Library of Science. <a href="https://doi.org/10.1371/journal.pone.0212699">https://doi.org/10.1371/journal.pone.0212699</a>
  chicago: Goudarzi, Mohammad, Aleix Boquet-Pujadas, Jean Christophe Olivo-Marin,
    and Erez Raz. “Fluid Dynamics during Bleb Formation in Migrating Cells in Vivo.”
    <i>PLOS ONE</i>. Public Library of Science, 2019. <a href="https://doi.org/10.1371/journal.pone.0212699">https://doi.org/10.1371/journal.pone.0212699</a>.
  ieee: M. Goudarzi, A. Boquet-Pujadas, J. C. Olivo-Marin, and E. Raz, “Fluid dynamics
    during bleb formation in migrating cells in vivo,” <i>PLOS ONE</i>, vol. 14, no.
    2. Public Library of Science, 2019.
  ista: Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. 2019. Fluid dynamics
    during bleb formation in migrating cells in vivo. PLOS ONE. 14(2), e0212699.
  mla: Goudarzi, Mohammad, et al. “Fluid Dynamics during Bleb Formation in Migrating
    Cells in Vivo.” <i>PLOS ONE</i>, vol. 14, no. 2, e0212699, Public Library of Science,
    2019, doi:<a href="https://doi.org/10.1371/journal.pone.0212699">10.1371/journal.pone.0212699</a>.
  short: M. Goudarzi, A. Boquet-Pujadas, J.C. Olivo-Marin, E. Raz, PLOS ONE 14 (2019).
date_created: 2019-03-10T22:59:21Z
date_published: 2019-02-26T00:00:00Z
date_updated: 2023-09-19T14:46:47Z
day: '26'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1371/journal.pone.0212699
external_id:
  isi:
  - '000459712100022'
file:
- access_level: open_access
  checksum: b885de050ed4bb3c86f706487a47197f
  content_type: application/pdf
  creator: dernst
  date_created: 2019-03-11T16:09:23Z
  date_updated: 2020-07-14T12:47:19Z
  file_id: '6096'
  file_name: 2019_PLoSOne_Goudarzi.pdf
  file_size: 2967731
  relation: main_file
file_date_updated: 2020-07-14T12:47:19Z
has_accepted_license: '1'
intvolume: '        14'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: PLOS ONE
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fluid dynamics during bleb formation in migrating cells in vivo
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 14
year: '2019'
...