---
_id: '10888'
abstract:
- lang: eng
  text: Despite the growing interest in using chemical genetics in plant research,
    small molecule target identification remains a major challenge. The cellular thermal
    shift assay coupled with high-resolution mass spectrometry (CETSA MS) that monitors
    changes in the thermal stability of proteins caused by their interactions with
    small molecules, other proteins, or posttranslational modifications, allows the
    discovery of drug targets or the study of protein–metabolite and protein–protein
    interactions mainly in mammalian cells. To showcase the applicability of this
    method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and
    identified the thermal proteome of the plant-specific glycogen synthase kinase
    3 (GSK3) inhibitor, bikinin. A comparison between the thermal and the phosphoproteomes
    of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a substrate
    of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling.
    We established that PIN1 phosphorylation by the GSK3s is essential for maintaining
    its intracellular polarity that is required for auxin-mediated regulation of vascular
    patterning in the leaf, thus revealing cross-talk between brassinosteroid and
    auxin signaling.
acknowledgement: "We thank Yanhai Yin for providing the anti-BES1 antibody, Johan
  Winne and Brenda Callebaut for synthesizing bikinin, Yuki Kondo and Hiroo Fukuda
  for published materials, Tomasz Nodzy\x03nski for useful advice, and Martine De
  Cock for help in preparing the manuscript. This\r\nwork was supported by the China
  Scholarship Council for predoctoral (Q.L. and X.X.) and postdoctoral (Y.Z.) fellowships;
  the Agency for Innovation by Science and Technology for a predoctoral fellowship
  (W.D.); the Research Foundation-Flanders, Projects G009018N and G002121N (E.R.);
  and the VIB TechWatch Fund (E.R.)."
article_number: e2118220119
article_processing_charge: No
article_type: original
author:
- first_name: Qing
  full_name: Lu, Qing
  last_name: Lu
- first_name: Yonghong
  full_name: Zhang, Yonghong
  last_name: Zhang
- first_name: Joakim
  full_name: Hellner, Joakim
  last_name: Hellner
- first_name: Caterina
  full_name: Giannini, Caterina
  id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
  last_name: Giannini
- first_name: Xiangyu
  full_name: Xu, Xiangyu
  last_name: Xu
- first_name: Jarne
  full_name: Pauwels, Jarne
  last_name: Pauwels
- first_name: Qian
  full_name: Ma, Qian
  last_name: Ma
- first_name: Wim
  full_name: Dejonghe, Wim
  last_name: Dejonghe
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Brigitte
  full_name: Van De Cotte, Brigitte
  last_name: Van De Cotte
- first_name: Francis
  full_name: Impens, Francis
  last_name: Impens
- first_name: Kris
  full_name: Gevaert, Kris
  last_name: Gevaert
- first_name: Ive
  full_name: De Smet, Ive
  last_name: De Smet
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Daniel Martinez
  full_name: Molina, Daniel Martinez
  last_name: Molina
- first_name: Eugenia
  full_name: Russinova, Eugenia
  last_name: Russinova
citation:
  ama: Lu Q, Zhang Y, Hellner J, et al. Proteome-wide cellular thermal shift assay
    reveals unexpected cross-talk between brassinosteroid and auxin signaling. <i>Proceedings
    of the National Academy of Sciences of the United States of America</i>. 2022;119(11).
    doi:<a href="https://doi.org/10.1073/pnas.2118220119">10.1073/pnas.2118220119</a>
  apa: Lu, Q., Zhang, Y., Hellner, J., Giannini, C., Xu, X., Pauwels, J., … Russinova,
    E. (2022). Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
    between brassinosteroid and auxin signaling. <i>Proceedings of the National Academy
    of Sciences of the United States of America</i>. Proceedings of the National Academy
    of Sciences. <a href="https://doi.org/10.1073/pnas.2118220119">https://doi.org/10.1073/pnas.2118220119</a>
  chicago: Lu, Qing, Yonghong Zhang, Joakim Hellner, Caterina Giannini, Xiangyu Xu,
    Jarne Pauwels, Qian Ma, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
    Cross-Talk between Brassinosteroid and Auxin Signaling.” <i>Proceedings of the
    National Academy of Sciences of the United States of America</i>. Proceedings
    of the National Academy of Sciences, 2022. <a href="https://doi.org/10.1073/pnas.2118220119">https://doi.org/10.1073/pnas.2118220119</a>.
  ieee: Q. Lu <i>et al.</i>, “Proteome-wide cellular thermal shift assay reveals unexpected
    cross-talk between brassinosteroid and auxin signaling,” <i>Proceedings of the
    National Academy of Sciences of the United States of America</i>, vol. 119, no.
    11. Proceedings of the National Academy of Sciences, 2022.
  ista: Lu Q, Zhang Y, Hellner J, Giannini C, Xu X, Pauwels J, Ma Q, Dejonghe W, Han
    H, Van De Cotte B, Impens F, Gevaert K, De Smet I, Friml J, Molina DM, Russinova
    E. 2022. Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
    between brassinosteroid and auxin signaling. Proceedings of the National Academy
    of Sciences of the United States of America. 119(11), e2118220119.
  mla: Lu, Qing, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
    Cross-Talk between Brassinosteroid and Auxin Signaling.” <i>Proceedings of the
    National Academy of Sciences of the United States of America</i>, vol. 119, no.
    11, e2118220119, Proceedings of the National Academy of Sciences, 2022, doi:<a
    href="https://doi.org/10.1073/pnas.2118220119">10.1073/pnas.2118220119</a>.
  short: Q. Lu, Y. Zhang, J. Hellner, C. Giannini, X. Xu, J. Pauwels, Q. Ma, W. Dejonghe,
    H. Han, B. Van De Cotte, F. Impens, K. Gevaert, I. De Smet, J. Friml, D.M. Molina,
    E. Russinova, Proceedings of the National Academy of Sciences of the United States
    of America 119 (2022).
date_created: 2022-03-20T23:01:39Z
date_published: 2022-03-07T00:00:00Z
date_updated: 2023-08-03T06:06:27Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.2118220119
external_id:
  isi:
  - '000771756300008'
  pmid:
  - '35254915'
file:
- access_level: open_access
  checksum: 83e0fea7919570d0b519b41193342571
  content_type: application/pdf
  creator: dernst
  date_created: 2022-03-21T09:19:47Z
  date_updated: 2022-03-21T09:19:47Z
  file_id: '10910'
  file_name: 2022_PNAS_Lu.pdf
  file_size: 2169534
  relation: main_file
  success: 1
file_date_updated: 2022-03-21T09:19:47Z
has_accepted_license: '1'
intvolume: '       119'
isi: 1
issue: '11'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
  of America
publication_identifier:
  eissn:
  - 1091-6490
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between
  brassinosteroid and auxin signaling
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '8931'
abstract:
- lang: eng
  text: "Auxin is a major plant growth regulator, but current models on auxin perception
    and signaling cannot explain the whole plethora of auxin effects, in particular
    those associated with rapid responses. A possible candidate for a component of
    additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1),
    whose function in planta remains unclear.\r\nHere we combined expression analysis
    with gain- and loss-of-function approaches to analyze the role of ABP1 in plant
    development. ABP1 shows a broad expression largely overlapping with, but not regulated
    by, transcriptional auxin response activity. Furthermore, ABP1 activity is not
    essential for the transcriptional auxin signaling. Genetic in planta analysis
    revealed that abp1 loss-of-function mutants show largely normal development with
    minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show
    a broad range of growth and developmental defects, including root and hypocotyl
    growth and bending, lateral root and leaf development, bolting, as well as response
    to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired
    auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular
    aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically
    unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function
    mutants by a functional redundancy."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We would like to acknowledge Bioimaging and Life Science Facilities
  at IST Austria for continuous support and also the Plant Sciences Core Facility
  of CEITEC Masaryk University for their support with obtaining a part of the scientific
  data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct
  design. This project has received funding from the European Research Council (ERC)
  under the European Union’s Horizon 2020 research and innovation program [grant agreement
  no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship
  of the Austrian Academy of Sciences to L.L.; the European Structural and Investment
  Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“
  [CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by
  the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738]
  to M. Č.
article_number: '110750'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Michelle C
  full_name: Gallei, Michelle C
  id: 35A03822-F248-11E8-B48F-1D18A9856A87
  last_name: Gallei
  orcid: 0000-0003-1286-7368
- first_name: Markéta
  full_name: Pernisová, Markéta
  last_name: Pernisová
- first_name: Géraldine
  full_name: Brunoud, Géraldine
  last_name: Brunoud
- first_name: Xixi
  full_name: Zhang, Xixi
  id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
  last_name: Zhang
  orcid: 0000-0001-7048-4627
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Jaroslav
  full_name: Michalko, Jaroslav
  id: 483727CA-F248-11E8-B48F-1D18A9856A87
  last_name: Michalko
- first_name: Zlata
  full_name: Pavlovicova, Zlata
  last_name: Pavlovicova
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Milada
  full_name: Čovanová, Milada
  last_name: Čovanová
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Lukas
  full_name: Hörmayer, Lukas
  id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
  last_name: Hörmayer
  orcid: 0000-0001-8295-2926
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Tongda
  full_name: Xu, Tongda
  last_name: Xu
- first_name: Teva
  full_name: Vernoux, Teva
  last_name: Vernoux
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding
    protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. 2021;303. doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110750">10.1016/j.plantsci.2020.110750</a>
  apa: Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M.,
    … Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis
    thaliana. <i>Plant Science</i>. Elsevier. <a href="https://doi.org/10.1016/j.plantsci.2020.110750">https://doi.org/10.1016/j.plantsci.2020.110750</a>
  chicago: Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud,
    Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding
    Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>. Elsevier, 2021. <a href="https://doi.org/10.1016/j.plantsci.2020.110750">https://doi.org/10.1016/j.plantsci.2020.110750</a>.
  ieee: Z. Gelová <i>et al.</i>, “Developmental roles of auxin binding protein 1 in
    Arabidopsis thaliana,” <i>Plant Science</i>, vol. 303. Elsevier, 2021.
  ista: Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko
    J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka
    M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles
    of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.
  mla: Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis
    Thaliana.” <i>Plant Science</i>, vol. 303, 110750, Elsevier, 2021, doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110750">10.1016/j.plantsci.2020.110750</a>.
  short: Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L.
    Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild,
    M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml,
    Plant Science 303 (2021).
date_created: 2020-12-09T14:48:28Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-10-29T10:22:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.1016/j.plantsci.2020.110750
ec_funded: 1
external_id:
  isi:
  - '000614154500001'
  pmid:
  - '33487339'
file:
- access_level: open_access
  checksum: a7f2562bdca62d67dfa88e271b62a629
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-04T07:49:25Z
  date_updated: 2021-02-04T07:49:25Z
  file_id: '9083'
  file_name: 2021_PlantScience_Gelova.pdf
  file_size: 12563728
  relation: main_file
  success: 1
file_date_updated: 2021-02-04T07:49:25Z
has_accepted_license: '1'
intvolume: '       303'
isi: 1
keyword:
- Agronomy and Crop Science
- Plant Science
- Genetics
- General Medicine
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
  grant_number: '25351'
  name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
    Rapid Growth Inhibition in Arabidopsis Root'
publication: Plant Science
publication_identifier:
  issn:
  - 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '11626'
    relation: dissertation_contains
    status: public
  - id: '10083'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Developmental roles of auxin binding protein 1 in Arabidopsis thaliana
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 303
year: '2021'
...
---
_id: '9287'
abstract:
- lang: eng
  text: "The phytohormone auxin and its directional transport through tissues are
    intensively studied. However, a mechanistic understanding of auxin-mediated feedback
    on endocytosis and polar distribution of PIN auxin transporters remains limited
    due to contradictory observations and interpretations. Here, we used state-of-the-art
    methods to reexamine the\r\nauxin effects on PIN endocytic trafficking. We used
    high auxin concentrations or longer treatments versus lower concentrations and
    shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish
    between specific and nonspecific effects. Longer treatments of both auxins interfere
    with Brefeldin A-mediated intracellular PIN2 accumulation and also with general
    aggregation of endomembrane compartments. NAA treatment decreased the internalization
    of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the
    number, distribution, and compartment identity of the early endosome/trans-Golgi
    network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations
    unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the
    endomembrane system, we opted for alternative approaches visualizing the endocytic
    events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence
    (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the
    incidence and dynamics of clathrin foci, implying that these treatments do not
    affect the overall endocytosis rate. However, both NAA and IAA at low concentrations
    rapidly and specifically promoted endocytosis of photo-converted PIN2 from the
    PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis,
    thus contributing to its\r\npolarity maintenance and furthermore illustrate that
    high auxin levels have nonspecific effects on trafficking and endomembrane compartments. "
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: 'We thank Ivan Kulik for developing the Chip’n’Dale apparatus with
  Lanxin Li; the IST machine shop and the Bioimaging facility for their excellent
  support; Matouš Glanc and Matyáš Fendrych for their valuable discussions and help;
  Barbara Casillas-Perez for her help with statistics. This project has received funding
  from the European Research Council (ERC) under the European Union''s Horizon 2020
  research and innovation program (grant agreement No 742985). A.J. is supported by
  funding from the Austrian Science Fund (FWF): I3630B25 to J.F. '
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Michelle C
  full_name: Gallei, Michelle C
  id: 35A03822-F248-11E8-B48F-1D18A9856A87
  last_name: Gallei
  orcid: 0000-0003-1286-7368
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Lesia
  full_name: Rodriguez Solovey, Lesia
  id: 3922B506-F248-11E8-B48F-1D18A9856A87
  last_name: Rodriguez Solovey
  orcid: 0000-0002-7244-7237
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: E
  full_name: Himschoot, E
  last_name: Himschoot
- first_name: R
  full_name: Wang, R
  last_name: Wang
- first_name: S
  full_name: Vanneste, S
  last_name: Vanneste
- first_name: J
  full_name: Sánchez-Simarro, J
  last_name: Sánchez-Simarro
- first_name: F
  full_name: Aniento, F
  last_name: Aniento
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Narasimhan M, Gallei MC, Tan S, et al. Systematic analysis of specific and
    nonspecific auxin effects on endocytosis and trafficking. <i>Plant Physiology</i>.
    2021;186(2):1122–1142. doi:<a href="https://doi.org/10.1093/plphys/kiab134">10.1093/plphys/kiab134</a>
  apa: Narasimhan, M., Gallei, M. C., Tan, S., Johnson, A. J., Verstraeten, I., Li,
    L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin
    effects on endocytosis and trafficking. <i>Plant Physiology</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/plphys/kiab134">https://doi.org/10.1093/plphys/kiab134</a>
  chicago: Narasimhan, Madhumitha, Michelle C Gallei, Shutang Tan, Alexander J Johnson,
    Inge Verstraeten, Lanxin Li, Lesia Rodriguez Solovey, et al. “Systematic Analysis
    of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” <i>Plant
    Physiology</i>. Oxford University Press, 2021. <a href="https://doi.org/10.1093/plphys/kiab134">https://doi.org/10.1093/plphys/kiab134</a>.
  ieee: M. Narasimhan <i>et al.</i>, “Systematic analysis of specific and nonspecific
    auxin effects on endocytosis and trafficking,” <i>Plant Physiology</i>, vol. 186,
    no. 2. Oxford University Press, pp. 1122–1142, 2021.
  ista: Narasimhan M, Gallei MC, Tan S, Johnson AJ, Verstraeten I, Li L, Rodriguez
    Solovey L, Han H, Himschoot E, Wang R, Vanneste S, Sánchez-Simarro J, Aniento
    F, Adamowski M, Friml J. 2021. Systematic analysis of specific and nonspecific
    auxin effects on endocytosis and trafficking. Plant Physiology. 186(2), 1122–1142.
  mla: Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific
    Auxin Effects on Endocytosis and Trafficking.” <i>Plant Physiology</i>, vol. 186,
    no. 2, Oxford University Press, 2021, pp. 1122–1142, doi:<a href="https://doi.org/10.1093/plphys/kiab134">10.1093/plphys/kiab134</a>.
  short: M. Narasimhan, M.C. Gallei, S. Tan, A.J. Johnson, I. Verstraeten, L. Li,
    L. Rodriguez Solovey, H. Han, E. Himschoot, R. Wang, S. Vanneste, J. Sánchez-Simarro,
    F. Aniento, M. Adamowski, J. Friml, Plant Physiology 186 (2021) 1122–1142.
date_created: 2021-03-26T12:08:38Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2024-10-29T10:22:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plphys/kiab134
ec_funded: 1
external_id:
  isi:
  - '000671555900031'
  pmid:
  - '33734402'
file:
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isi: 1
issue: '2'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1122–1142
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
  eissn:
  - 1532-2548
  issn:
  - 0032-0889
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
  link:
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    url: 10.1093/plphys/kiab380
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status: public
title: Systematic analysis of specific and nonspecific auxin effects on endocytosis
  and trafficking
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 186
year: '2021'
...
---
_id: '9288'
abstract:
- lang: eng
  text: "• The phenylpropanoid pathway serves a central role in plant metabolism,
    providing numerous compounds involved in diverse physiological processes. Most
    carbon entering the pathway is incorporated into lignin. Although several phenylpropanoid
    pathway mutants show seedling growth arrest, the role for lignin in seedling growth
    and development is unexplored.\r\n• We use complementary pharmacological and genetic
    approaches to block CINNAMATE‐4‐HYDROXYLASE (C4H) functionality in Arabidopsis
    seedlings and a set of molecular and biochemical techniques to investigate the
    underlying phenotypes.\r\n• Blocking C4H resulted in reduced lateral rooting and
    increased adventitious rooting apically in the hypocotyl. These phenotypes coincided
    with an inhibition in auxin transport. The upstream accumulation in cis‐cinnamic
    acid was found to likely cause polar auxin transport inhibition. Conversely, a
    downstream depletion in lignin perturbed phloem‐mediated auxin transport. Restoring
    lignin deposition effectively reestablished phloem transport and, accordingly,
    auxin homeostasis.\r\n• Our results show that the accumulation of bioactive intermediates
    and depletion in lignin jointly cause the aberrant phenotypes upon blocking C4H,
    and demonstrate that proper deposition of lignin is essential for the establishment
    of auxin distribution in seedlings. Our data position the phenylpropanoid pathway
    and lignin in a new physiological framework, consolidating their importance in
    plant growth and development."
article_processing_charge: No
article_type: original
author:
- first_name: I
  full_name: El Houari, I
  last_name: El Houari
- first_name: C
  full_name: Van Beirs, C
  last_name: Van Beirs
- first_name: HE
  full_name: Arents, HE
  last_name: Arents
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: A
  full_name: Chanoca, A
  last_name: Chanoca
- first_name: D
  full_name: Opdenacker, D
  last_name: Opdenacker
- first_name: J
  full_name: Pollier, J
  last_name: Pollier
- first_name: V
  full_name: Storme, V
  last_name: Storme
- first_name: W
  full_name: Steenackers, W
  last_name: Steenackers
- first_name: M
  full_name: Quareshy, M
  last_name: Quareshy
- first_name: R
  full_name: Napier, R
  last_name: Napier
- first_name: T
  full_name: Beeckman, T
  last_name: Beeckman
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: B
  full_name: De Rybel, B
  last_name: De Rybel
- first_name: W
  full_name: Boerjan, W
  last_name: Boerjan
- first_name: B
  full_name: Vanholme, B
  last_name: Vanholme
citation:
  ama: El Houari I, Van Beirs C, Arents H, et al. Seedling developmental defects upon
    blocking CINNAMATE-4-HYDROXYLASE are caused by perturbations in auxin transport.
    <i>New Phytologist</i>. 2021;230(6):2275-2291. doi:<a href="https://doi.org/10.1111/nph.17349">10.1111/nph.17349</a>
  apa: El Houari, I., Van Beirs, C., Arents, H., Han, H., Chanoca, A., Opdenacker,
    D., … Vanholme, B. (2021). Seedling developmental defects upon blocking CINNAMATE-4-HYDROXYLASE
    are caused by perturbations in auxin transport. <i>New Phytologist</i>. Wiley.
    <a href="https://doi.org/10.1111/nph.17349">https://doi.org/10.1111/nph.17349</a>
  chicago: El Houari, I, C Van Beirs, HE Arents, Huibin Han, A Chanoca, D Opdenacker,
    J Pollier, et al. “Seedling Developmental Defects upon Blocking CINNAMATE-4-HYDROXYLASE
    Are Caused by Perturbations in Auxin Transport.” <i>New Phytologist</i>. Wiley,
    2021. <a href="https://doi.org/10.1111/nph.17349">https://doi.org/10.1111/nph.17349</a>.
  ieee: I. El Houari <i>et al.</i>, “Seedling developmental defects upon blocking
    CINNAMATE-4-HYDROXYLASE are caused by perturbations in auxin transport,” <i>New
    Phytologist</i>, vol. 230, no. 6. Wiley, pp. 2275–2291, 2021.
  ista: El Houari I, Van Beirs C, Arents H, Han H, Chanoca A, Opdenacker D, Pollier
    J, Storme V, Steenackers W, Quareshy M, Napier R, Beeckman T, Friml J, De Rybel
    B, Boerjan W, Vanholme B. 2021. Seedling developmental defects upon blocking CINNAMATE-4-HYDROXYLASE
    are caused by perturbations in auxin transport. New Phytologist. 230(6), 2275–2291.
  mla: El Houari, I., et al. “Seedling Developmental Defects upon Blocking CINNAMATE-4-HYDROXYLASE
    Are Caused by Perturbations in Auxin Transport.” <i>New Phytologist</i>, vol.
    230, no. 6, Wiley, 2021, pp. 2275–91, doi:<a href="https://doi.org/10.1111/nph.17349">10.1111/nph.17349</a>.
  short: I. El Houari, C. Van Beirs, H. Arents, H. Han, A. Chanoca, D. Opdenacker,
    J. Pollier, V. Storme, W. Steenackers, M. Quareshy, R. Napier, T. Beeckman, J.
    Friml, B. De Rybel, W. Boerjan, B. Vanholme, New Phytologist 230 (2021) 2275–2291.
date_created: 2021-03-26T12:09:01Z
date_published: 2021-03-17T00:00:00Z
date_updated: 2023-09-05T15:46:55Z
day: '17'
department:
- _id: JiFr
doi: 10.1111/nph.17349
external_id:
  isi:
  - '000639552400001'
  pmid:
  - '33728703'
intvolume: '       230'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://biblio.ugent.be/publication/8703799/file/8703800.pdf
month: '03'
oa: 1
oa_version: Published Version
page: 2275-2291
pmid: 1
publication: New Phytologist
publication_identifier:
  eissn:
  - 1469-8137
  issn:
  - 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Seedling developmental defects upon blocking CINNAMATE-4-HYDROXYLASE are caused
  by perturbations in auxin transport
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 230
year: '2021'
...
---
_id: '9656'
abstract:
- lang: eng
  text: Tropisms, growth responses to environmental stimuli such as light or gravity,
    are spectacular examples of adaptive plant development. The plant hormone auxin
    serves as a major coordinative signal. The PIN auxin exporters, through their
    dynamic polar subcellular localizations, redirect auxin fluxes in response to
    environmental stimuli and the resulting auxin gradients across organs underly
    differential cell elongation and bending. In this review, we discuss recent advances
    concerning regulations of PIN polarity during tropisms, focusing on PIN phosphorylation
    and trafficking. We also cover how environmental cues regulate PIN actions during
    tropisms, and a crucial role of auxin feedback on PIN polarity during bending
    termination. Finally, the interactions between different tropisms are reviewed
    to understand plant adaptive growth in the natural environment.
acknowledgement: We are grateful to Lukas Fiedler, Alexandra Mally (IST Austria) and
  Dr. Bartel Vanholme (VIB, Ghent) for their critical comments on the manuscript.
  We apologize to those researchers whose great work was not cited. This work is supported
  by the European Research Council under the European Union’s Horizon 2020 research
  and innovation Programme (ERC grant agreement number 742985), and the Austrian Science
  Fund (FWF, grant number I 3630-B25) to JF. HH is supported by the China Scholarship
  Council (CSC scholarship, 201506870018) and a starting grant from Jiangxi Agriculture
  University (9232308314).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Linlin
  full_name: Qi, Linlin
  id: 44B04502-A9ED-11E9-B6FC-583AE6697425
  last_name: Qi
  orcid: 0000-0001-5187-8401
- first_name: SS
  full_name: Alotaibi, SS
  last_name: Alotaibi
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. PIN-mediated polar auxin transport
    regulations in plant tropic responses. <i>New Phytologist</i>. 2021;232(2):510-522.
    doi:<a href="https://doi.org/10.1111/nph.17617">10.1111/nph.17617</a>
  apa: Han, H., Adamowski, M., Qi, L., Alotaibi, S., &#38; Friml, J. (2021). PIN-mediated
    polar auxin transport regulations in plant tropic responses. <i>New Phytologist</i>.
    Wiley. <a href="https://doi.org/10.1111/nph.17617">https://doi.org/10.1111/nph.17617</a>
  chicago: Han, Huibin, Maciek Adamowski, Linlin Qi, SS Alotaibi, and Jiří Friml.
    “PIN-Mediated Polar Auxin Transport Regulations in Plant Tropic Responses.” <i>New
    Phytologist</i>. Wiley, 2021. <a href="https://doi.org/10.1111/nph.17617">https://doi.org/10.1111/nph.17617</a>.
  ieee: H. Han, M. Adamowski, L. Qi, S. Alotaibi, and J. Friml, “PIN-mediated polar
    auxin transport regulations in plant tropic responses,” <i>New Phytologist</i>,
    vol. 232, no. 2. Wiley, pp. 510–522, 2021.
  ista: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. 2021. PIN-mediated polar auxin
    transport regulations in plant tropic responses. New Phytologist. 232(2), 510–522.
  mla: Han, Huibin, et al. “PIN-Mediated Polar Auxin Transport Regulations in Plant
    Tropic Responses.” <i>New Phytologist</i>, vol. 232, no. 2, Wiley, 2021, pp. 510–22,
    doi:<a href="https://doi.org/10.1111/nph.17617">10.1111/nph.17617</a>.
  short: H. Han, M. Adamowski, L. Qi, S. Alotaibi, J. Friml, New Phytologist 232 (2021)
    510–522.
date_created: 2021-07-14T15:29:14Z
date_published: 2021-10-01T00:00:00Z
date_updated: 2023-08-10T14:02:41Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.17617
ec_funded: 1
external_id:
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  - '000680587100001'
  pmid:
  - '34254313'
file:
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  checksum: 6422a6eb329b52d96279daaee0fcf189
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  creator: kschuh
  date_created: 2021-10-07T13:42:47Z
  date_updated: 2021-10-07T13:42:47Z
  file_id: '10105'
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month: '10'
oa: 1
oa_version: Published Version
page: 510-522
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: New Phytologist
publication_identifier:
  eissn:
  - 1469-8137
  issn:
  - 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-mediated polar auxin transport regulations in plant tropic responses
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 232
year: '2021'
...
---
_id: '8589'
abstract:
- lang: eng
  text: The plant hormone auxin plays indispensable roles in plant growth and development.
    An essential level of regulation in auxin action is the directional auxin transport
    within cells. The establishment of auxin gradient in plant tissue has been attributed
    to local auxin biosynthesis and directional intercellular auxin transport, which
    both are controlled by various environmental and developmental signals. It is
    well established that asymmetric auxin distribution in cells is achieved by polarly
    localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights
    into cellular mechanisms of PIN polarization obtained from the last decades, the
    molecular mechanism and specific regulators mediating PIN polarization remains
    elusive. In this thesis, we aim to find novel players in PIN subcellular polarity
    regulation during Arabidopsis development. We first characterize the physiological
    effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and
    PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling
    pathway in shoot gravitropism bending termination. In addition, we also explore
    the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation
    on PIN polarity. In Chapter 1, we give an overview of the current knowledge about
    PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we
    study the physiological effect of PA on shoot gravitropic bending. Our results
    show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering
    with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending
    hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear
    auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization
    and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics
    approach and identify the motor protein Myosin XI and its binding protein, the
    MadB2 family, as an essential regulator of PIN polarity for auxin-canalization
    related developmental processes. In Chapter 5, we demonstrate the vital role of
    actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular
    trafficking. Overall, the data presented in this PhD thesis brings novel insights
    into the PIN polar localization regulation that resulted in the (re)establishment
    of the polar auxin flow and gradient in response to environmental stimuli during
    plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: I also want to thank the China Scholarship Council for supporting
  my study during the year from 2015 to 2019. I also want to thank IST facilities
  – the Bioimaging facility, the media kitchen, the plant facility and all of the
  campus services, for their support.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
citation:
  ama: Han H. Novel insights into PIN polarity regulation during Arabidopsis development.
    2020. doi:<a href="https://doi.org/10.15479/AT:ISTA:8589">10.15479/AT:ISTA:8589</a>
  apa: Han, H. (2020). <i>Novel insights into PIN polarity regulation during Arabidopsis
    development</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:8589">https://doi.org/10.15479/AT:ISTA:8589</a>
  chicago: Han, Huibin. “Novel Insights into PIN Polarity Regulation during Arabidopsis
    Development.” Institute of Science and Technology Austria, 2020. <a href="https://doi.org/10.15479/AT:ISTA:8589">https://doi.org/10.15479/AT:ISTA:8589</a>.
  ieee: H. Han, “Novel insights into PIN polarity regulation during Arabidopsis development,”
    Institute of Science and Technology Austria, 2020.
  ista: Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis
    development. Institute of Science and Technology Austria.
  mla: Han, Huibin. <i>Novel Insights into PIN Polarity Regulation during Arabidopsis
    Development</i>. Institute of Science and Technology Austria, 2020, doi:<a href="https://doi.org/10.15479/AT:ISTA:8589">10.15479/AT:ISTA:8589</a>.
  short: H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development,
    Institute of Science and Technology Austria, 2020.
date_created: 2020-09-30T14:50:51Z
date_published: 2020-09-30T00:00:00Z
date_updated: 2023-09-07T13:13:05Z
day: '30'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:8589
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publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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  - id: '7643'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
title: Novel insights into PIN polarity regulation during Arabidopsis development
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...
---
_id: '7465'
abstract:
- lang: eng
  text: The flexible development of plants is characterized by a high capacity for
    post-embryonic organ formation and tissue regeneration, processes, which require
    tightly regulated intercellular communication and coordinated tissue (re-)polarization.
    The phytohormone auxin, the main driver for these processes, is able to establish
    polarized auxin transport channels, which are characterized by the expression
    and polar, subcellular localization of the PIN1 auxin transport proteins. These
    channels are demarcating the position of future vascular strands necessary for
    organ formation and tissue regeneration. Major progress has been made in the last
    years to understand how PINs can change their polarity in different contexts and
    thus guide auxin flow through the plant. However, it still remains elusive how
    auxin mediates the establishment of auxin conducting channels and the formation
    of vascular tissue and which cellular processes are involved. By the means of
    sophisticated regeneration experiments combined with local auxin applications
    in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics,
    (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact
    actin cytoskeleton required for post-endocytic trafficking are indispensable for
    auxin channel formation, de novo vascular formation and vascular regeneration
    after wounding. These observations provide novel insights into cellular mechanism
    of coordinated tissue polarization during auxin canalization.
article_number: '110414'
article_processing_charge: No
article_type: original
author:
- first_name: Ewa
  full_name: Mazur, Ewa
  last_name: Mazur
- first_name: Michelle C
  full_name: Gallei, Michelle C
  id: 35A03822-F248-11E8-B48F-1D18A9856A87
  last_name: Gallei
  orcid: 0000-0003-1286-7368
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Hélène S.
  full_name: Robert, Hélène S.
  last_name: Robert
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated
    trafficking and PIN trafficking are required for auxin canalization and vascular
    tissue formation in Arabidopsis. <i>Plant Science</i>. 2020;293(4). doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110414">10.1016/j.plantsci.2020.110414</a>
  apa: Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., &#38; Friml,
    J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for
    auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.plantsci.2020.110414">https://doi.org/10.1016/j.plantsci.2020.110414</a>
  chicago: Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S.
    Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are
    Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.”
    <i>Plant Science</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.plantsci.2020.110414">https://doi.org/10.1016/j.plantsci.2020.110414</a>.
  ieee: E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml,
    “Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
    and vascular tissue formation in Arabidopsis,” <i>Plant Science</i>, vol. 293,
    no. 4. Elsevier, 2020.
  ista: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated
    trafficking and PIN trafficking are required for auxin canalization and vascular
    tissue formation in Arabidopsis. Plant Science. 293(4), 110414.
  mla: Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required
    for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant
    Science</i>, vol. 293, no. 4, 110414, Elsevier, 2020, doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110414">10.1016/j.plantsci.2020.110414</a>.
  short: E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant
    Science 293 (2020).
date_created: 2020-02-09T23:00:50Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:37:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2020.110414
ec_funded: 1
external_id:
  isi:
  - '000520609800009'
file:
- access_level: open_access
  checksum: f7f27c6a8fea985ceb9279be2204461c
  content_type: application/pdf
  creator: dernst
  date_created: 2020-02-10T08:59:36Z
  date_updated: 2020-07-14T12:47:59Z
  file_id: '7471'
  file_name: 2020_PlantScience_Mazur.pdf
  file_size: 3499069
  relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: '       293'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Science
publication_identifier:
  eissn:
  - '18732259'
  issn:
  - '01689452'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '11626'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
  and vascular tissue formation in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 293
year: '2020'
...
---
_id: '7643'
acknowledgement: 'This work was supported by the European Research Council under the
  European Union’s Horizon 2020 research and innovation Programme (ERC grant agreement
  number 742985), and the Austrian Science Fund (FWF, grant number I 3630-B25) to
  JF. HH is supported by the China Scholarship Council (CSC scholarship). '
article_processing_charge: No
article_type: letter_note
author:
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Hana
  full_name: Rakusova, Hana
  id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
  last_name: Rakusova
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Yuzhou
  full_name: Zhang, Yuzhou
  id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
  last_name: Zhang
  orcid: 0000-0003-2627-6956
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. SCF TIR1/AFB auxin signaling
    for bending termination during shoot gravitropism. <i>Plant Physiology</i>. 2020;183(5):37-40.
    doi:<a href="https://doi.org/10.1104/pp.20.00212">10.1104/pp.20.00212</a>
  apa: Han, H., Rakusova, H., Verstraeten, I., Zhang, Y., &#38; Friml, J. (2020).
    SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism.
    <i>Plant Physiology</i>. American Society of Plant Biologists. <a href="https://doi.org/10.1104/pp.20.00212">https://doi.org/10.1104/pp.20.00212</a>
  chicago: Han, Huibin, Hana Rakusova, Inge Verstraeten, Yuzhou Zhang, and Jiří Friml.
    “SCF TIR1/AFB Auxin Signaling for Bending Termination during Shoot Gravitropism.”
    <i>Plant Physiology</i>. American Society of Plant Biologists, 2020. <a href="https://doi.org/10.1104/pp.20.00212">https://doi.org/10.1104/pp.20.00212</a>.
  ieee: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, and J. Friml, “SCF TIR1/AFB
    auxin signaling for bending termination during shoot gravitropism,” <i>Plant Physiology</i>,
    vol. 183, no. 5. American Society of Plant Biologists, pp. 37–40, 2020.
  ista: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. 2020. SCF TIR1/AFB auxin
    signaling for bending termination during shoot gravitropism. Plant Physiology.
    183(5), 37–40.
  mla: Han, Huibin, et al. “SCF TIR1/AFB Auxin Signaling for Bending Termination during
    Shoot Gravitropism.” <i>Plant Physiology</i>, vol. 183, no. 5, American Society
    of Plant Biologists, 2020, pp. 37–40, doi:<a href="https://doi.org/10.1104/pp.20.00212">10.1104/pp.20.00212</a>.
  short: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, J. Friml, Plant Physiology
    183 (2020) 37–40.
date_created: 2020-04-06T10:06:40Z
date_published: 2020-05-08T00:00:00Z
date_updated: 2023-09-07T13:13:04Z
day: '08'
department:
- _id: JiFr
doi: 10.1104/pp.20.00212
ec_funded: 1
external_id:
  isi:
  - '000536641800018'
  pmid:
  - '32107280'
intvolume: '       183'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1104/pp.20.00212
month: '05'
oa: 1
oa_version: Published Version
page: 37-40
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
  eissn:
  - 1532-2548
  issn:
  - 0032-0889
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
related_material:
  record:
  - id: '8589'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 183
year: '2020'
...
---
_id: '6262'
abstract:
- lang: eng
  text: "Gravitropism is an adaptive response that orients plant growth parallel to
    the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a
    necessary prerequisite to the tropic bending both in roots and\r\nshoots. During
    hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells
    to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization
    to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower
    side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization
    restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed
    a forward genetic screen to identify regulators of both PIN3 polarization events
    during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations
    based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced
    hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb)
    and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated
    typically with defective gravity-induced PIN3 relocation whereas all analyzed
    hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation
    sequencing-aided mutation map-\r\nping identified several candidate genes, including
    SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin
    cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe
    hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms
    underlying cell\r\npolarity and plant adaptive development."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hana
  full_name: Rakusová, Hana
  last_name: Rakusová
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Petr
  full_name: Valošek, Petr
  id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
  last_name: Valošek
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating
    PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant
    Journal</i>. 2019;98(6):1048-1059. doi:<a href="https://doi.org/10.1111/tpj.14301">10.1111/tpj.14301</a>
  apa: Rakusová, H., Han, H., Valošek, P., &#38; Friml, J. (2019). Genetic screen
    for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana
    hypocotyls. <i>The Plant Journal</i>. Wiley. <a href="https://doi.org/10.1111/tpj.14301">https://doi.org/10.1111/tpj.14301</a>
  chicago: Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen
    for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana
    Hypocotyls.” <i>The Plant Journal</i>. Wiley, 2019. <a href="https://doi.org/10.1111/tpj.14301">https://doi.org/10.1111/tpj.14301</a>.
  ieee: H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors
    mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,”
    <i>The Plant Journal</i>, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.
  ista: Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating
    PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
    Journal. 98(6), 1048–1059.
  mla: Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization
    in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>,
    vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:<a href="https://doi.org/10.1111/tpj.14301">10.1111/tpj.14301</a>.
  short: H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059.
date_created: 2019-04-09T08:46:44Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2025-05-07T11:12:30Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/tpj.14301
ec_funded: 1
external_id:
  isi:
  - '000473644100008'
  pmid:
  - '30821050'
file:
- access_level: open_access
  checksum: ad3b5e270b67ba2a45f894ce3be27920
  content_type: application/pdf
  creator: dernst
  date_created: 2019-04-15T09:38:43Z
  date_updated: 2020-07-14T12:47:25Z
  file_id: '6304'
  file_name: 2019_PlantJournal_Rakusov.pdf
  file_size: 1383100
  relation: main_file
file_date_updated: 2020-07-14T12:47:25Z
has_accepted_license: '1'
intvolume: '        98'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1048-1059
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: The Plant Journal
publication_identifier:
  eissn:
  - 1365-313x
  issn:
  - 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis
  thaliana hypocotyls
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 98
year: '2019'
...
---
_id: '5830'
abstract:
- lang: eng
  text: CLE peptides have been implicated in various developmental processes of plants
    and mediate their responses to environmental stimuli. However, the biological
    relevance of most CLE genes remains to be functionally characterized. Here, we
    report that CLE9, which is expressed in stomata, acts as an essential regulator
    in the induction of stomatal closure. Exogenous application of CLE9 peptides or
    overexpression of CLE9 effectively led to stomatal closure and enhanced drought
    tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress.
    CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants,
    indicating that ABA is required for CLE9-medaited guard cell signalling. We further
    deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were
    responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by
    the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants.
    In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2)
    and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished
    in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively.
    Collectively, our results reveal a novel ABA-dependent function of CLE9 in the
    regulation of stomatal apertures, thereby suggesting a potential role of CLE9
    in the stress acclimatization of plants.
article_processing_charge: No
author:
- first_name: Luosha
  full_name: Zhang, Luosha
  last_name: Zhang
- first_name: Xiong
  full_name: Shi, Xiong
  last_name: Shi
- first_name: Yutao
  full_name: Zhang, Yutao
  last_name: Zhang
- first_name: Jiajing
  full_name: Wang, Jiajing
  last_name: Wang
- first_name: Jingwei
  full_name: Yang, Jingwei
  last_name: Yang
- first_name: Takashi
  full_name: Ishida, Takashi
  last_name: Ishida
- first_name: Wenqian
  full_name: Jiang, Wenqian
  last_name: Jiang
- first_name: Xiangyu
  full_name: Han, Xiangyu
  last_name: Han
- first_name: Jingke
  full_name: Kang, Jingke
  last_name: Kang
- first_name: Xuening
  full_name: Wang, Xuening
  last_name: Wang
- first_name: Lixia
  full_name: Pan, Lixia
  last_name: Pan
- first_name: Shuo
  full_name: Lv, Shuo
  last_name: Lv
- first_name: Bing
  full_name: Cao, Bing
  last_name: Cao
- first_name: Yonghong
  full_name: Zhang, Yonghong
  last_name: Zhang
- first_name: Jinbin
  full_name: Wu, Jinbin
  last_name: Wu
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Zhubing
  full_name: Hu, Zhubing
  last_name: Hu
- first_name: Langjun
  full_name: Cui, Langjun
  last_name: Cui
- first_name: Shinichiro
  full_name: Sawa, Shinichiro
  last_name: Sawa
- first_name: Junmin
  full_name: He, Junmin
  last_name: He
- first_name: Guodong
  full_name: Wang, Guodong
  last_name: Wang
citation:
  ama: Zhang L, Shi X, Zhang Y, et al. CLE9 peptide-induced stomatal closure is mediated
    by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana.
    <i>Plant Cell and Environment</i>. 2018. doi:<a href="https://doi.org/10.1111/pce.13475">10.1111/pce.13475</a>
  apa: Zhang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., … Wang, G. (2018).
    CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide,
    and nitric oxide in arabidopsis thaliana. <i>Plant Cell and Environment</i>. Wiley.
    <a href="https://doi.org/10.1111/pce.13475">https://doi.org/10.1111/pce.13475</a>
  chicago: Zhang, Luosha, Xiong Shi, Yutao Zhang, Jiajing Wang, Jingwei Yang, Takashi
    Ishida, Wenqian Jiang, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated
    by Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.”
    <i>Plant Cell and Environment</i>. Wiley, 2018. <a href="https://doi.org/10.1111/pce.13475">https://doi.org/10.1111/pce.13475</a>.
  ieee: L. Zhang <i>et al.</i>, “CLE9 peptide-induced stomatal closure is mediated
    by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana,”
    <i>Plant Cell and Environment</i>. Wiley, 2018.
  ista: Zhang L, Shi X, Zhang Y, Wang J, Yang J, Ishida T, Jiang W, Han X, Kang J,
    Wang X, Pan L, Lv S, Cao B, Zhang Y, Wu J, Han H, Hu Z, Cui L, Sawa S, He J, Wang
    G. 2018. CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
    peroxide, and nitric oxide in arabidopsis thaliana. Plant Cell and Environment.
  mla: Zhang, Luosha, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated by
    Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.” <i>Plant
    Cell and Environment</i>, Wiley, 2018, doi:<a href="https://doi.org/10.1111/pce.13475">10.1111/pce.13475</a>.
  short: L. Zhang, X. Shi, Y. Zhang, J. Wang, J. Yang, T. Ishida, W. Jiang, X. Han,
    J. Kang, X. Wang, L. Pan, S. Lv, B. Cao, Y. Zhang, J. Wu, H. Han, Z. Hu, L. Cui,
    S. Sawa, J. He, G. Wang, Plant Cell and Environment (2018).
date_created: 2019-01-13T22:59:11Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-11T12:43:31Z
day: '31'
department:
- _id: JiFr
doi: 10.1111/pce.13475
external_id:
  isi:
  - '000459014800021'
  pmid:
  - '30378140'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pubmed/30378140
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Cell and Environment
publication_identifier:
  issn:
  - '01407791'
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
  peroxide, and nitric oxide in arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '510'
abstract:
- lang: eng
  text: 'The CLE (CLAVATA3/Embryo Surrounding Region-related) peptides are small secreted
    signaling peptides that are primarily involved in the regulation of stem cell
    homeostasis in different plant meristems. Particularly, the characterization of
    the CLE41-PXY/TDR signaling pathway has greatly advanced our understanding on
    the potential roles of CLE peptides in vascular development and wood formation.
    Nevertheless, our knowledge on this gene family in a tree species is limited.
    In a recent study, we reported on a systematically investigation of the CLE gene
    family in Populus trichocarpa . The potential roles of PtCLE genes were studied
    by comparative analysis and transcriptional pro fi ling. Among fi fty PtCLE members,
    many PtCLE proteins share identical CLE motifs or contain the same CLE motif as
    that of AtCLEs, while PtCLE genes exhibited either comparable or distinct expression
    patterns comparing to their Arabidopsis counterparts. These fi ndings indicate
    the existence of both functional conservation and functional divergence between
    PtCLEs and their AtCLE orthologues. Our results provide valuable resources for
    future functional investigations of these critical signaling molecules in woody
    plants. '
acknowledgement: 'We are grateful to Dr. Long (Laboratoire de Reproduction et Developpement
  des Plantes,CNRS,INRA,ENSLyon,UCBL,Universite de Lyon,France)for critical reading
  of the article. Work in our group is supported by the National Natural Science Foundation
  of China (31271575; 31200902), the Fundamental Research Funds for the Central Univ
  ersities (GK201103005), the Specialized Research Fund for the Doctoral Program of
  Higher Education from the Ministry of Education of China (20120202120009), the Scientific
  Research Foundation for the Returned Overseas Chinese Scholars, State Education
  Ministry, and the Natural Science Basic Research Plan in Shaanxi Province of China
  (2014JM3064). '
article_number: e1191734
article_processing_charge: No
author:
- first_name: Zhijun
  full_name: Liu, Zhijun
  last_name: Liu
- first_name: 'Nan'
  full_name: Yang, Nan
  last_name: Yang
- first_name: Yanting
  full_name: Lv, Yanting
  last_name: Lv
- first_name: Lixia
  full_name: Pan, Lixia
  last_name: Pan
- first_name: Shuo
  full_name: Lv, Shuo
  last_name: Lv
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Guodong
  full_name: Wang, Guodong
  last_name: Wang
citation:
  ama: Liu Z, Yang N, Lv Y, et al. The CLE gene family in Populus trichocarpa. <i>Plant
    Signaling &#38; Behavior</i>. 2016;11(6). doi:<a href="https://doi.org/10.1080/15592324.2016.1191734">10.1080/15592324.2016.1191734</a>
  apa: Liu, Z., Yang, N., Lv, Y., Pan, L., Lv, S., Han, H., &#38; Wang, G. (2016).
    The CLE gene family in Populus trichocarpa. <i>Plant Signaling &#38; Behavior</i>.
    Taylor &#38; Francis. <a href="https://doi.org/10.1080/15592324.2016.1191734">https://doi.org/10.1080/15592324.2016.1191734</a>
  chicago: Liu, Zhijun, Nan Yang, Yanting Lv, Lixia Pan, Shuo Lv, Huibin Han, and
    Guodong Wang. “The CLE Gene Family in Populus Trichocarpa.” <i>Plant Signaling
    &#38; Behavior</i>. Taylor &#38; Francis, 2016. <a href="https://doi.org/10.1080/15592324.2016.1191734">https://doi.org/10.1080/15592324.2016.1191734</a>.
  ieee: Z. Liu <i>et al.</i>, “The CLE gene family in Populus trichocarpa,” <i>Plant
    Signaling &#38; Behavior</i>, vol. 11, no. 6. Taylor &#38; Francis, 2016.
  ista: Liu Z, Yang N, Lv Y, Pan L, Lv S, Han H, Wang G. 2016. The CLE gene family
    in Populus trichocarpa. Plant Signaling &#38; Behavior. 11(6), e1191734.
  mla: Liu, Zhijun, et al. “The CLE Gene Family in Populus Trichocarpa.” <i>Plant
    Signaling &#38; Behavior</i>, vol. 11, no. 6, e1191734, Taylor &#38; Francis,
    2016, doi:<a href="https://doi.org/10.1080/15592324.2016.1191734">10.1080/15592324.2016.1191734</a>.
  short: Z. Liu, N. Yang, Y. Lv, L. Pan, S. Lv, H. Han, G. Wang, Plant Signaling &#38;
    Behavior 11 (2016).
date_created: 2018-12-11T11:46:53Z
date_published: 2016-06-02T00:00:00Z
date_updated: 2023-10-17T11:13:40Z
day: '02'
department:
- _id: JiFr
doi: 10.1080/15592324.2016.1191734
intvolume: '        11'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973754/
month: '06'
oa: 1
oa_version: Submitted Version
publication: Plant Signaling & Behavior
publication_status: published
publisher: Taylor & Francis
publist_id: '7308'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The CLE gene family in Populus trichocarpa
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2016'
...
---
_id: '1212'
abstract:
- lang: eng
  text: 'Plants adjust their growth according to gravity. Gravitropism involves gravity
    perception, signal transduction, and asymmetric growth response, with organ bending
    as a consequence [1]. Asymmetric growth results from the asymmetric distribution
    of the plant-specific signaling molecule auxin [2] that is generated by lateral
    transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3
    (PIN3) [3–5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal
    cells, correlating with increased auxin accumulation in adjacent tissues at the
    lower side of the stimulated organ, where auxin induces cell elongation and, hence,
    organ bending. A curvature response allows the hypocotyl to resume straight growth
    at a defined angle [6], implying that at some point auxin symmetry is restored
    to prevent overbending. Here, we present initial insights into cellular and molecular
    mechanisms that lead to the termination of the tropic response. We identified
    an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry
    of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization
    events redirect auxin fluxes at different time points of the gravity response:
    first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower
    hypocotyl side, where auxin gradually accumulates and promotes growth, and later
    PIN3 polarization to the opposite cell side, depleting this auxin maximum to end
    the bending. Accordingly, genetic or pharmacological interference with the late
    PIN3 polarization prevents termination of the response and leads to hypocotyl
    overbending. This observation reveals a role of auxin feedback on PIN polarity
    in the termination of the tropic response. © 2016 Elsevier Ltd'
acknowledgement: "We thank Dr. Jie Li (Key Laboratory of Plant Molecular Physiology,
  Chinese Academy of Science, China) for the pPIN3::PIN3-GFP/DII::VENUS line and Martine
  De Cock for help in preparing the manuscript. This work was supported by the European
  Research Council (project ERC-2011-StG-20101109-PSDP), by the Czech Science Foundation
  GAČR (GA13-40637S) to J.F., and by the Ministry of Education, Youth and Sports of
  the Czech Republic under the project CEITEC 2020 (LQ1601) to H.S.R. H.R. is indebted
  to the Agency for Innovation by Science and Technology (IWT) for a predoctoral fellowship.\r\n"
author:
- first_name: Hana
  full_name: Rakusová, Hana
  last_name: Rakusová
- first_name: Mohamad
  full_name: Abbas, Mohamad
  id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
  last_name: Abbas
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Siyuan
  full_name: Song, Siyuan
  last_name: Song
- first_name: Hélène
  full_name: Robert, Hélène
  last_name: Robert
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. Termination of shoot
    gravitropic responses by auxin feedback on PIN3 polarity. <i>Current Biology</i>.
    2016;26(22):3026-3032. doi:<a href="https://doi.org/10.1016/j.cub.2016.08.067">10.1016/j.cub.2016.08.067</a>
  apa: Rakusová, H., Abbas, M., Han, H., Song, S., Robert, H., &#38; Friml, J. (2016).
    Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity.
    <i>Current Biology</i>. Cell Press. <a href="https://doi.org/10.1016/j.cub.2016.08.067">https://doi.org/10.1016/j.cub.2016.08.067</a>
  chicago: Rakusová, Hana, Mohamad Abbas, Huibin Han, Siyuan Song, Hélène Robert,
    and Jiří Friml. “Termination of Shoot Gravitropic Responses by Auxin Feedback
    on PIN3 Polarity.” <i>Current Biology</i>. Cell Press, 2016. <a href="https://doi.org/10.1016/j.cub.2016.08.067">https://doi.org/10.1016/j.cub.2016.08.067</a>.
  ieee: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, and J. Friml, “Termination
    of shoot gravitropic responses by auxin feedback on PIN3 polarity,” <i>Current
    Biology</i>, vol. 26, no. 22. Cell Press, pp. 3026–3032, 2016.
  ista: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. 2016. Termination of
    shoot gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
    26(22), 3026–3032.
  mla: Rakusová, Hana, et al. “Termination of Shoot Gravitropic Responses by Auxin
    Feedback on PIN3 Polarity.” <i>Current Biology</i>, vol. 26, no. 22, Cell Press,
    2016, pp. 3026–32, doi:<a href="https://doi.org/10.1016/j.cub.2016.08.067">10.1016/j.cub.2016.08.067</a>.
  short: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology
    26 (2016) 3026–3032.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-21T00:00:00Z
date_updated: 2021-01-12T06:49:08Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2016.08.067
ec_funded: 1
file:
- access_level: open_access
  checksum: 79ed2498185a027cf51a8f88100379e6
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:09:33Z
  date_updated: 2020-07-14T12:44:39Z
  file_id: '4757'
  file_name: IST-2018-1008-v1+1_Rakusova_CurrBiol_2016_proof.pdf
  file_size: 5391923
  relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: '        26'
issue: '22'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 3026 - 3032
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '6138'
pubrep_id: '1008'
quality_controlled: '1'
scopus_import: 1
status: public
title: Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...