[{"author":[{"last_name":"Inglés Prieto","full_name":"Inglés Prieto, Álvaro","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5409-8571","first_name":"Álvaro"},{"first_name":"Eva","orcid":"0000-0002-7218-7738","full_name":"Gschaider-Reichhart, Eva","id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","last_name":"Gschaider-Reichhart"},{"last_name":"Muellner","full_name":"Muellner, Markus","first_name":"Markus"},{"first_name":"Matthias","last_name":"Nowak","id":"30845DAA-F248-11E8-B48F-1D18A9856A87","full_name":"Nowak, Matthias"},{"first_name":"Sebastian","full_name":"Nijman, Sebastian","last_name":"Nijman"},{"first_name":"Michael","last_name":"Grusch","full_name":"Grusch, Michael"},{"last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","full_name":"Janovjak, Harald L","first_name":"Harald L","orcid":"0000-0002-8023-9315"}],"day":"12","scopus_import":1,"oa_version":"Submitted Version","title":"Light-assisted small-molecule screening against protein kinases","volume":11,"date_created":"2018-12-11T11:53:25Z","has_accepted_license":"1","intvolume":"        11","abstract":[{"lang":"eng","text":"High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes."}],"publication_status":"published","file_date_updated":"2020-07-14T12:45:12Z","month":"10","department":[{"_id":"HaJa"},{"_id":"LifeSc"}],"file":[{"file_id":"4842","creator":"system","date_updated":"2020-07-14T12:45:12Z","date_created":"2018-12-12T10:10:51Z","file_size":1308364,"checksum":"e9fb251dfcb7cd209b83f17867e61321","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2017-837-v1+1_ingles-prieto.pdf"}],"oa":1,"language":[{"iso":"eng"}],"pubrep_id":"837","issue":"12","citation":{"ama":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. 2015;11(12):952-954. doi:<a href=\"https://doi.org/10.1038/nchembio.1933\">10.1038/nchembio.1933</a>","ieee":"Á. Inglés Prieto <i>et al.</i>, “Light-assisted small-molecule screening against protein kinases,” <i>Nature Chemical Biology</i>, vol. 11, no. 12. Nature Publishing Group, pp. 952–954, 2015.","short":"Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman, M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954.","chicago":"Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nchembio.1933\">https://doi.org/10.1038/nchembio.1933</a>.","ista":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 11(12), 952–954.","apa":"Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman, S., Grusch, M., &#38; Janovjak, H. L. (2015). Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nchembio.1933\">https://doi.org/10.1038/nchembio.1933</a>","mla":"Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>, vol. 11, no. 12, Nature Publishing Group, 2015, pp. 952–54, doi:<a href=\"https://doi.org/10.1038/nchembio.1933\">10.1038/nchembio.1933</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1038/nchembio.1933","publisher":"Nature Publishing Group","date_updated":"2023-09-07T12:49:09Z","_id":"1678","type":"journal_article","page":"952 - 954","ddc":["571"],"quality_controlled":"1","year":"2015","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"418"}]},"publist_id":"5471","project":[{"call_identifier":"FP7","grant_number":"303564","name":"Microbial Ion Channels for Synthetic Neurobiology","_id":"25548C20-B435-11E9-9278-68D0E5697425"},{"grant_number":"RGY0084/2012","name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)","_id":"255BFFFA-B435-11E9-9278-68D0E5697425"},{"_id":"255A6082-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"W1232-B24","name":"Molecular Drug Targets"}],"publication":"Nature Chemical Biology","status":"public","ec_funded":1,"date_published":"2015-10-12T00:00:00Z","acknowledgement":"This work was supported by grants from the European Union Seventh Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech fellowship (3580812 Austrian Research Promotion Agency)."},{"date_created":"2018-12-11T11:54:20Z","article_type":"original","volume":137,"oa_version":"Submitted Version","title":"FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors","scopus_import":1,"day":"01","author":[{"last_name":"Schwamb","full_name":"Schwamb, Bettina","first_name":"Bettina"},{"last_name":"Pick","full_name":"Pick, Robert","first_name":"Robert"},{"first_name":"Sara","last_name":"Fernández","full_name":"Fernández, Sara"},{"first_name":"Kirsten","last_name":"Völp","full_name":"Völp, Kirsten"},{"last_name":"Heering","full_name":"Heering, Jan","first_name":"Jan"},{"last_name":"Dötsch","full_name":"Dötsch, Volker","first_name":"Volker"},{"first_name":"Susanne","last_name":"Bösser","full_name":"Bösser, Susanne"},{"last_name":"Jung","full_name":"Jung, Jennifer","first_name":"Jennifer"},{"first_name":"Rasa","last_name":"Beinoravičiute Kellner","full_name":"Beinoravičiute Kellner, Rasa"},{"first_name":"Josephine","full_name":"Wesely, Josephine","last_name":"Wesely"},{"full_name":"Zörnig, Inka","last_name":"Zörnig","first_name":"Inka"},{"full_name":"Hammerschmidt, Matthias","last_name":"Hammerschmidt","first_name":"Matthias"},{"last_name":"Nowak","id":"30845DAA-F248-11E8-B48F-1D18A9856A87","full_name":"Nowak, Matthias","first_name":"Matthias"},{"last_name":"Penzel","full_name":"Penzel, Roland","first_name":"Roland"},{"last_name":"Zatloukal","full_name":"Zatloukal, Kurt","first_name":"Kurt"},{"first_name":"Stefan","last_name":"Joos","full_name":"Joos, Stefan"},{"first_name":"Ralf","last_name":"Rieker","full_name":"Rieker, Ralf"},{"first_name":"Abbas","last_name":"Agaimy","full_name":"Agaimy, Abbas"},{"full_name":"Söder, Stephan","last_name":"Söder","first_name":"Stephan"},{"first_name":"Kmarie","last_name":"Reid Lombardo","full_name":"Reid Lombardo, Kmarie"},{"first_name":"Michael","last_name":"Kendrick","full_name":"Kendrick, Michael"},{"last_name":"Bardsley","full_name":"Bardsley, Michael","first_name":"Michael"},{"last_name":"Hayashi","full_name":"Hayashi, Yujiro","first_name":"Yujiro"},{"first_name":"David","last_name":"Asuzu","full_name":"Asuzu, David"},{"last_name":"Syed","full_name":"Syed, Sabriya","first_name":"Sabriya"},{"last_name":"Ördög","full_name":"Ördög, Tamás","first_name":"Tamás"},{"full_name":"Zörnig, Martin","last_name":"Zörnig","first_name":"Martin"}],"publication_status":"published","intvolume":"       137","abstract":[{"text":"The ability to escape apoptosis is a hallmark of cancer-initiating cells and a key factor of resistance to oncolytic therapy. Here, we identify FAM96A as a ubiquitous, evolutionarily conserved apoptosome-activating protein and investigate its potential pro-apoptotic tumor suppressor function in gastrointestinal stromal tumors (GISTs). Interaction between FAM96A and apoptotic peptidase activating factor 1 (APAF1) was identified in yeast two-hybrid screen and further studied by deletion mutants, glutathione-S-transferase pull-down, co-immunoprecipitation and immunofluorescence. Effects of FAM96A overexpression and knock-down on apoptosis sensitivity were examined in cancer cells and zebrafish embryos. Expression of FAM96A in GISTs and histogenetically related cells including interstitial cells of Cajal (ICCs), “fibroblast-like cells” (FLCs) and ICC stem cells (ICC-SCs) was investigated by Northern blotting, reverse transcription—polymerase chain reaction, immunohistochemistry and Western immunoblotting. Tumorigenicity of GIST cells and transformed murine ICC-SCs stably transduced to re-express FAM96A was studied by xeno- and allografting into immunocompromised mice. FAM96A was found to bind APAF1 and to enhance the induction of mitochondrial apoptosis. FAM96A protein or mRNA was dramatically reduced or lost in 106 of 108 GIST samples representing three independent patient cohorts. Whereas ICCs, ICC-SCs and FLCs, the presumed normal counterparts of GIST, were found to robustly express FAM96A protein and mRNA, FAM96A expression was much reduced in tumorigenic ICC-SCs. Re-expression of FAM96A in GIST cells and transformed ICC-SCs increased apoptosis sensitivity and diminished tumorigenicity. Our data suggest FAM96A is a novel pro-apoptotic tumor suppressor that is lost during GIST tumorigenesis.","lang":"eng"}],"department":[{"_id":"LifeSc"}],"month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"B. Schwamb, R. Pick, S. Fernández, K. Völp, J. Heering, V. Dötsch, S. Bösser, J. Jung, R. Beinoravičiute Kellner, J. Wesely, I. Zörnig, M. Hammerschmidt, M. Nowak, R. Penzel, K. Zatloukal, S. Joos, R. Rieker, A. Agaimy, S. Söder, K. Reid Lombardo, M. Kendrick, M. Bardsley, Y. Hayashi, D. Asuzu, S. Syed, T. Ördög, M. Zörnig, International Journal of Cancer 137 (2015) 1318–1329.","ieee":"B. Schwamb <i>et al.</i>, “FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors,” <i>International Journal of Cancer</i>, vol. 137, no. 6. Wiley, pp. 1318–1329, 2015.","ama":"Schwamb B, Pick R, Fernández S, et al. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. <i>International Journal of Cancer</i>. 2015;137(6):1318-1329. doi:<a href=\"https://doi.org/10.1002/ijc.29498\">10.1002/ijc.29498</a>","mla":"Schwamb, Bettina, et al. “FAM96A Is a Novel Pro-Apoptotic Tumor Suppressor in Gastrointestinal Stromal Tumors.” <i>International Journal of Cancer</i>, vol. 137, no. 6, Wiley, 2015, pp. 1318–29, doi:<a href=\"https://doi.org/10.1002/ijc.29498\">10.1002/ijc.29498</a>.","apa":"Schwamb, B., Pick, R., Fernández, S., Völp, K., Heering, J., Dötsch, V., … Zörnig, M. (2015). FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. <i>International Journal of Cancer</i>. Wiley. <a href=\"https://doi.org/10.1002/ijc.29498\">https://doi.org/10.1002/ijc.29498</a>","chicago":"Schwamb, Bettina, Robert Pick, Sara Fernández, Kirsten Völp, Jan Heering, Volker Dötsch, Susanne Bösser, et al. “FAM96A Is a Novel Pro-Apoptotic Tumor Suppressor in Gastrointestinal Stromal Tumors.” <i>International Journal of Cancer</i>. Wiley, 2015. <a href=\"https://doi.org/10.1002/ijc.29498\">https://doi.org/10.1002/ijc.29498</a>.","ista":"Schwamb B, Pick R, Fernández S, Völp K, Heering J, Dötsch V, Bösser S, Jung J, Beinoravičiute Kellner R, Wesely J, Zörnig I, Hammerschmidt M, Nowak M, Penzel R, Zatloukal K, Joos S, Rieker R, Agaimy A, Söder S, Reid Lombardo K, Kendrick M, Bardsley M, Hayashi Y, Asuzu D, Syed S, Ördög T, Zörnig M. 2015. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer. 137(6), 1318–1329."},"issue":"6","language":[{"iso":"eng"}],"oa":1,"type":"journal_article","_id":"1848","date_updated":"2021-01-12T06:53:36Z","publisher":"Wiley","article_processing_charge":"No","doi":"10.1002/ijc.29498","quality_controlled":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497860/","open_access":"1"}],"page":"1318 - 1329","publist_id":"5253","external_id":{"pmid":["25716227"]},"year":"2015","date_published":"2015-09-01T00:00:00Z","pmid":1,"publication":"International Journal of Cancer","status":"public"}]