---
_id: '6506'
abstract:
- lang: eng
  text: How does environmental complexity affect the evolution of single genes? Here,
    we measured the effects of a set of Bacillus subtilis glutamate dehydrogenase
    mutants across 19 different environments—from phenotypically homogeneous single-cell
    populations in liquid media to heterogeneous biofilms, plant roots and soil populations.
    The effects of individual gene mutations on organismal fitness were highly reproducible
    in liquid cultures. However, 84% of the tested alleles showed opposing fitness
    effects under different growth conditions (sign environmental pleiotropy). In
    colony biofilms and soil samples, different alleles dominated in parallel replica
    experiments. Accordingly, we found that in these heterogeneous cell populations
    the fate of mutations was dictated by a combination of selection and drift. The
    latter relates to programmed prophage excisions that occurred during biofilm development.
    Overall, for each condition, a wide range of glutamate dehydrogenase mutations
    persisted and sometimes fixated as a result of the combined action of selection,
    pleiotropy and chance. However, over longer periods and in multiple environments,
    nearly all of this diversity would be lost—across all the environments and conditions
    that we tested, the wild type was the fittest allele.
article_processing_charge: No
article_type: original
author:
- first_name: Lianet
  full_name: Noda-García, Lianet
  last_name: Noda-García
- first_name: Dan
  full_name: Davidi, Dan
  last_name: Davidi
- first_name: Elisa
  full_name: Korenblum, Elisa
  last_name: Korenblum
- first_name: Assaf
  full_name: Elazar, Assaf
  last_name: Elazar
- first_name: Ekaterina
  full_name: Putintseva, Ekaterina
  id: 2EF67C84-F248-11E8-B48F-1D18A9856A87
  last_name: Putintseva
- first_name: Asaph
  full_name: Aharoni, Asaph
  last_name: Aharoni
- first_name: Dan S.
  full_name: Tawfik, Dan S.
  last_name: Tawfik
citation:
  ama: Noda-García L, Davidi D, Korenblum E, et al. Chance and pleiotropy dominate
    genetic diversity in complex bacterial environments. <i>Nature Microbiology</i>.
    2019;4(7):1221–1230. doi:<a href="https://doi.org/10.1038/s41564-019-0412-y">10.1038/s41564-019-0412-y</a>
  apa: Noda-García, L., Davidi, D., Korenblum, E., Elazar, A., Putintseva, E., Aharoni,
    A., &#38; Tawfik, D. S. (2019). Chance and pleiotropy dominate genetic diversity
    in complex bacterial environments. <i>Nature Microbiology</i>. Springer Nature.
    <a href="https://doi.org/10.1038/s41564-019-0412-y">https://doi.org/10.1038/s41564-019-0412-y</a>
  chicago: Noda-García, Lianet, Dan Davidi, Elisa Korenblum, Assaf Elazar, Ekaterina
    Putintseva, Asaph Aharoni, and Dan S. Tawfik. “Chance and Pleiotropy Dominate
    Genetic Diversity in Complex Bacterial Environments.” <i>Nature Microbiology</i>.
    Springer Nature, 2019. <a href="https://doi.org/10.1038/s41564-019-0412-y">https://doi.org/10.1038/s41564-019-0412-y</a>.
  ieee: L. Noda-García <i>et al.</i>, “Chance and pleiotropy dominate genetic diversity
    in complex bacterial environments,” <i>Nature Microbiology</i>, vol. 4, no. 7.
    Springer Nature, pp. 1221–1230, 2019.
  ista: Noda-García L, Davidi D, Korenblum E, Elazar A, Putintseva E, Aharoni A, Tawfik
    DS. 2019. Chance and pleiotropy dominate genetic diversity in complex bacterial
    environments. Nature Microbiology. 4(7), 1221–1230.
  mla: Noda-García, Lianet, et al. “Chance and Pleiotropy Dominate Genetic Diversity
    in Complex Bacterial Environments.” <i>Nature Microbiology</i>, vol. 4, no. 7,
    Springer Nature, 2019, pp. 1221–1230, doi:<a href="https://doi.org/10.1038/s41564-019-0412-y">10.1038/s41564-019-0412-y</a>.
  short: L. Noda-García, D. Davidi, E. Korenblum, A. Elazar, E. Putintseva, A. Aharoni,
    D.S. Tawfik, Nature Microbiology 4 (2019) 1221–1230.
date_created: 2019-05-29T13:03:30Z
date_published: 2019-07-01T00:00:00Z
date_updated: 2023-08-28T08:39:47Z
day: '01'
department:
- _id: FyKo
doi: 10.1038/s41564-019-0412-y
external_id:
  isi:
  - '000480348200017'
intvolume: '         4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/340828v2
month: '07'
oa: 1
oa_version: Preprint
page: 1221–1230
publication: Nature Microbiology
publication_identifier:
  issn:
  - 2058-5276
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chance and pleiotropy dominate genetic diversity in complex bacterial environments
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 4
year: '2019'
...