---
_id: '1912'
abstract:
- lang: eng
  text: Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the
    embryo along its left-right (LR) axis. Regional differences in cell shape within
    the lumen-lining KV epithelium are essential for its LR patterning function. However,
    the processes by which KV cells acquire their characteristic shapes are largely
    unknown. Here, we show that the notochord induces regional differences in cell
    shape within KV by triggering extracellular matrix (ECM) accumulation adjacent
    to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts
    apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen
    growth. Our study provides mechanistic insight into the processes by which KV
    translates global embryonic patterning into regional cell shape differences required
    for its LR symmetry-breaking function.
acknowledgement: We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus,
  and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing
  reagents. This work was supported by the Institute of Science and Technology Austria
  and an Alexander von Humboldt Foundation fellowship to J.C.
article_processing_charge: No
author:
- first_name: Julien
  full_name: Compagnon, Julien
  id: 2E3E0988-F248-11E8-B48F-1D18A9856A87
  last_name: Compagnon
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Srivarsha
  full_name: Rajshekar, Srivarsha
  last_name: Rajshekar
- first_name: Rita
  full_name: Kottmeier, Rita
  last_name: Kottmeier
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry
    by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental
    Cell</i>. 2014;31(6):774-783. doi:<a href="https://doi.org/10.1016/j.devcel.2014.11.003">10.1016/j.devcel.2014.11.003</a>
  apa: Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K.,
    Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). The notochord breaks bilateral
    symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental
    Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.devcel.2014.11.003">https://doi.org/10.1016/j.devcel.2014.11.003</a>
  chicago: Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier,
    Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The
    Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish
    Laterality Organ.” <i>Developmental Cell</i>. Cell Press, 2014. <a href="https://doi.org/10.1016/j.devcel.2014.11.003">https://doi.org/10.1016/j.devcel.2014.11.003</a>.
  ieee: J. Compagnon <i>et al.</i>, “The notochord breaks bilateral symmetry by controlling
    cell shapes in the Zebrafish laterality organ,” <i>Developmental Cell</i>, vol.
    31, no. 6. Cell Press, pp. 774–783, 2014.
  ista: Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt
    M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling
    cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783.
  mla: Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling
    Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>, vol.
    31, no. 6, Cell Press, 2014, pp. 774–83, doi:<a href="https://doi.org/10.1016/j.devcel.2014.11.003">10.1016/j.devcel.2014.11.003</a>.
  short: J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha,
    M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.
date_created: 2018-12-11T11:54:41Z
date_published: 2014-12-22T00:00:00Z
date_updated: 2023-09-07T12:05:08Z
day: '22'
department:
- _id: CaHe
doi: 10.1016/j.devcel.2014.11.003
external_id:
  pmid:
  - '25535919'
intvolume: '        31'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pubmed/25535919
month: '12'
oa: 1
oa_version: Published Version
page: 774 - 783
pmid: 1
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '5182'
quality_controlled: '1'
related_material:
  record:
  - id: '961'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish
  laterality organ
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 31
year: '2014'
...
---
_id: '2862'
abstract:
- lang: eng
  text: Motile cilia perform crucial functions during embryonic development and throughout
    adult life. Development of organs containing motile cilia involves regulation
    of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis)
    in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis
    is not yet fully understood, and it remains unclear whether these processes are
    coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently
    in ciliated organs. Lgl proteins are involved in establishing cell polarity and
    have been implicated in vesicle trafficking. Here, we identified a role for Lgl2
    in development of ciliated epithelia in Kupffer's vesicle, which directs left-right
    asymmetry of the embryo; the otic vesicles, which give rise to the inner ear;
    and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated
    organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia
    number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle
    morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed
    loss of the adherens junction component E-cadherin at lateral membranes. Genetic
    interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin
    and mediate lumen formation that is uncoupled from cilia formation. These results
    uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis
    and ciliogenesis and indicate that these processes are genetically separable in
    zebrafish.
acknowledgement: Deposited in PMC for release after 12 months. We thank members of
  the Amack lab for helpful discussions and Mahendra Sonawane for donating reagents.
author:
- first_name: Hwee
  full_name: Tay, Hwee
  last_name: Tay
- first_name: Sabrina
  full_name: Schulze, Sabrina
  last_name: Schulze
- first_name: Julien
  full_name: Compagnon, Julien
  id: 2E3E0988-F248-11E8-B48F-1D18A9856A87
  last_name: Compagnon
- first_name: Fiona
  full_name: Foley, Fiona
  last_name: Foley
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: H Joseph
  full_name: Yost, H Joseph
  last_name: Yost
- first_name: Salim
  full_name: Abdelilah Seyfried, Salim
  last_name: Abdelilah Seyfried
- first_name: Jeffrey
  full_name: Amack, Jeffrey
  last_name: Amack
citation:
  ama: Tay H, Schulze S, Compagnon J, et al. Lethal giant larvae 2 regulates development
    of the ciliated organ Kupffer’s vesicle. <i>Development</i>. 2013;140(7):1550-1559.
    doi:<a href="https://doi.org/10.1242/dev.087130">10.1242/dev.087130</a>
  apa: Tay, H., Schulze, S., Compagnon, J., Foley, F., Heisenberg, C.-P. J., Yost,
    H. J., … Amack, J. (2013). Lethal giant larvae 2 regulates development of the
    ciliated organ Kupffer’s vesicle. <i>Development</i>. Company of Biologists. <a
    href="https://doi.org/10.1242/dev.087130">https://doi.org/10.1242/dev.087130</a>
  chicago: Tay, Hwee, Sabrina Schulze, Julien Compagnon, Fiona Foley, Carl-Philipp
    J Heisenberg, H Joseph Yost, Salim Abdelilah Seyfried, and Jeffrey Amack. “Lethal
    Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.”
    <i>Development</i>. Company of Biologists, 2013. <a href="https://doi.org/10.1242/dev.087130">https://doi.org/10.1242/dev.087130</a>.
  ieee: H. Tay <i>et al.</i>, “Lethal giant larvae 2 regulates development of the
    ciliated organ Kupffer’s vesicle,” <i>Development</i>, vol. 140, no. 7. Company
    of Biologists, pp. 1550–1559, 2013.
  ista: Tay H, Schulze S, Compagnon J, Foley F, Heisenberg C-PJ, Yost HJ, Abdelilah
    Seyfried S, Amack J. 2013. Lethal giant larvae 2 regulates development of the
    ciliated organ Kupffer’s vesicle. Development. 140(7), 1550–1559.
  mla: Tay, Hwee, et al. “Lethal Giant Larvae 2 Regulates Development of the Ciliated
    Organ Kupffer’s Vesicle.” <i>Development</i>, vol. 140, no. 7, Company of Biologists,
    2013, pp. 1550–59, doi:<a href="https://doi.org/10.1242/dev.087130">10.1242/dev.087130</a>.
  short: H. Tay, S. Schulze, J. Compagnon, F. Foley, C.-P.J. Heisenberg, H.J. Yost,
    S. Abdelilah Seyfried, J. Amack, Development 140 (2013) 1550–1559.
date_created: 2018-12-11T11:59:59Z
date_published: 2013-04-01T00:00:00Z
date_updated: 2021-01-12T07:00:20Z
day: '01'
department:
- _id: CaHe
doi: 10.1242/dev.087130
external_id:
  pmid:
  - '23482490'
intvolume: '       140'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596994/
month: '04'
oa: 1
oa_version: Submitted Version
page: 1550 - 1559
pmid: 1
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '3927'
quality_controlled: '1'
scopus_import: 1
status: public
title: Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s
  vesicle
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 140
year: '2013'
...
---
_id: '2920'
abstract:
- lang: eng
  text: Cell polarisation in development is a common and fundamental process underlying
    embryo patterning and morphogenesis, and has been extensively studied over the
    past years. Our current knowledge of cell polarisation in development is predominantly
    based on studies that have analysed polarisation of single cells, such as eggs,
    or cellular aggregates with a stable polarising interface, such as cultured epithelial
    cells (St Johnston and Ahringer, 2010). However, in embryonic development, particularly
    of vertebrates, cell polarisation processes often encompass large numbers of cells
    that are placed within moving and proliferating tissues, and undergo mesenchymal-to-epithelial
    transitions with a highly complex spatiotemporal choreography. How such intricate
    cell polarisation processes in embryonic development are achieved has only started
    to be analysed. By using live imaging of neurulation in the transparent zebrafish
    embryo, Buckley et al (2012) now describe a novel polarisation strategy by which
    cells assemble an apical domain in the part of their cell body that intersects
    with the midline of the forming neural rod. This mechanism, along with the previously
    described mirror-symmetric divisions (Tawk et al, 2007), is thought to trigger
    formation of both neural rod midline and lumen.
author:
- first_name: Julien
  full_name: Compagnon, Julien
  id: 2E3E0988-F248-11E8-B48F-1D18A9856A87
  last_name: Compagnon
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Compagnon J, Heisenberg C-PJ. Neurulation coordinating cell polarisation and
    lumen formation. <i>EMBO Journal</i>. 2013;32(1):1-3. doi:<a href="https://doi.org/10.1038/emboj.2012.325">10.1038/emboj.2012.325</a>
  apa: Compagnon, J., &#38; Heisenberg, C.-P. J. (2013). Neurulation coordinating
    cell polarisation and lumen formation. <i>EMBO Journal</i>. Wiley-Blackwell. <a
    href="https://doi.org/10.1038/emboj.2012.325">https://doi.org/10.1038/emboj.2012.325</a>
  chicago: Compagnon, Julien, and Carl-Philipp J Heisenberg. “Neurulation Coordinating
    Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>. Wiley-Blackwell,
    2013. <a href="https://doi.org/10.1038/emboj.2012.325">https://doi.org/10.1038/emboj.2012.325</a>.
  ieee: J. Compagnon and C.-P. J. Heisenberg, “Neurulation coordinating cell polarisation
    and lumen formation,” <i>EMBO Journal</i>, vol. 32, no. 1. Wiley-Blackwell, pp.
    1–3, 2013.
  ista: Compagnon J, Heisenberg C-PJ. 2013. Neurulation coordinating cell polarisation
    and lumen formation. EMBO Journal. 32(1), 1–3.
  mla: Compagnon, Julien, and Carl-Philipp J. Heisenberg. “Neurulation Coordinating
    Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>, vol. 32, no. 1, Wiley-Blackwell,
    2013, pp. 1–3, doi:<a href="https://doi.org/10.1038/emboj.2012.325">10.1038/emboj.2012.325</a>.
  short: J. Compagnon, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 1–3.
date_created: 2018-12-11T12:00:20Z
date_published: 2013-01-09T00:00:00Z
date_updated: 2021-01-12T07:00:42Z
day: '09'
department:
- _id: CaHe
doi: 10.1038/emboj.2012.325
external_id:
  pmid:
  - '23211745'
intvolume: '        32'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545307/
month: '01'
oa: 1
oa_version: Submitted Version
page: 1 - 3
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3817'
quality_controlled: '1'
scopus_import: 1
status: public
title: Neurulation coordinating cell polarisation and lumen formation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2013'
...