---
_id: '12830'
abstract:
- lang: eng
  text: Interstitial fluid (IF) accumulation between embryonic cells is thought to
    be important for embryo patterning and morphogenesis. Here, we identify a positive
    mechanical feedback loop between cell migration and IF relocalization and find
    that it promotes embryonic axis formation during zebrafish gastrulation. We show
    that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between
    the yolk cell and deep cell tissue to extend the embryonic axis, compress the
    overlying deep cell layer, thereby causing IF to flow from the deep cell layer
    to the boundary between the yolk cell and the deep cell layer, directly ahead
    of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion
    formation and migration by opening up the space into which the ppl moves and,
    thereby, the ability of the ppl to trigger IF relocalization by pushing against
    the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic
    feedback loop between cell migration and IF relocalization.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful
  discussions and support with the SPIM experiments; the Heisenberg group, and especially
  Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović
  (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and
  zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia
  Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura
  (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work
  was supported by funding from the European Union (European Research Council Advanced
  grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and
  HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des
  deutschen Volkes to F.P.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Diana C
  full_name: Nunes Pinheiro, Diana C
  id: 2E839F16-F248-11E8-B48F-1D18A9856A87
  last_name: Nunes Pinheiro
  orcid: 0000-0003-4333-7503
- first_name: Friedrich
  full_name: Preusser, Friedrich
  last_name: Preusser
- first_name: Irene
  full_name: Steccari, Irene
  id: 2705C766-9FE2-11EA-B224-C6773DDC885E
  last_name: Steccari
- first_name: Christoph M
  full_name: Sommer, Christoph M
  id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
  last_name: Sommer
  orcid: 0000-0003-1216-9105
- first_name: Suyash
  full_name: Naik, Suyash
  id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
  last_name: Naik
  orcid: 0000-0001-8421-5508
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop
    between mesendoderm cell migration and interstitial fluid relocalization promotes
    embryonic axis formation in zebrafish. <i>Developmental Cell</i>. 2023;58(7):582-596.e7.
    doi:<a href="https://doi.org/10.1016/j.devcel.2023.02.016">10.1016/j.devcel.2023.02.016</a>
  apa: Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I.,
    Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between
    mesendoderm cell migration and interstitial fluid relocalization promotes embryonic
    axis formation in zebrafish. <i>Developmental Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.devcel.2023.02.016">https://doi.org/10.1016/j.devcel.2023.02.016</a>
  chicago: Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser,
    Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg.
    “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial
    Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental
    Cell</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.devcel.2023.02.016">https://doi.org/10.1016/j.devcel.2023.02.016</a>.
  ieee: K. Huljev <i>et al.</i>, “A hydraulic feedback loop between mesendoderm cell
    migration and interstitial fluid relocalization promotes embryonic axis formation
    in zebrafish,” <i>Developmental Cell</i>, vol. 58, no. 7. Elsevier, p. 582–596.e7,
    2023.
  ista: Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM,
    Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell
    migration and interstitial fluid relocalization promotes embryonic axis formation
    in zebrafish. Developmental Cell. 58(7), 582–596.e7.
  mla: Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration
    and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.”
    <i>Developmental Cell</i>, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:<a
    href="https://doi.org/10.1016/j.devcel.2023.02.016">10.1016/j.devcel.2023.02.016</a>.
  short: K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M.
    Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.
date_created: 2023-04-16T22:01:07Z
date_published: 2023-04-10T00:00:00Z
date_updated: 2023-08-01T14:10:38Z
day: '10'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1016/j.devcel.2023.02.016
ec_funded: 1
external_id:
  isi:
  - '000982111800001'
file:
- access_level: open_access
  checksum: c80ca2ebc241232aacdb5aa4b4c80957
  content_type: application/pdf
  creator: dernst
  date_created: 2023-04-17T07:41:25Z
  date_updated: 2023-04-17T07:41:25Z
  file_id: '12842'
  file_name: 2023_DevelopmentalCell_Huljev.pdf
  file_size: 7925886
  relation: main_file
  success: 1
file_date_updated: 2023-04-17T07:41:25Z
has_accepted_license: '1'
intvolume: '        58'
isi: 1
issue: '7'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 582-596.e7
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 850-2017
  name: Coordination of mesendoderm cell fate specification and internalization during
    zebrafish gastrulation
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
  grant_number: LT000429
  name: Coordination of mesendoderm fate specification and internalization during
    zebrafish gastrulation
publication: Developmental Cell
publication_identifier:
  eissn:
  - 1878-1551
  issn:
  - 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A hydraulic feedback loop between mesendoderm cell migration and interstitial
  fluid relocalization promotes embryonic axis formation in zebrafish
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 58
year: '2023'
...