---
_id: '665'
abstract:
- lang: eng
  text: The molecular mechanisms underlying phenotypic variation in isogenic bacterial
    populations remain poorly understood.We report that AcrAB-TolC, the main multidrug
    efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell
    poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex
    formation. Mother cells inheriting old poles are phenotypically distinct and display
    increased drug efflux activity relative to daughters. Consequently, we find systematic
    and long-lived growth differences between mother and daughter cells in the presence
    of subinhibitory drug concentrations. A simple model for biased partitioning predicts
    a population structure of long-lived and highly heterogeneous phenotypes. This
    straightforward mechanism of generating sustained growth rate differences at subinhibitory
    antibiotic concentrations has implications for understanding the emergence of
    multidrug resistance in bacteria.
article_processing_charge: No
article_type: original
author:
- first_name: Tobias
  full_name: Bergmiller, Tobias
  id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
  last_name: Bergmiller
  orcid: 0000-0001-5396-4346
- first_name: Anna M
  full_name: Andersson, Anna M
  id: 2B8A40DA-F248-11E8-B48F-1D18A9856A87
  last_name: Andersson
  orcid: 0000-0003-2912-6769
- first_name: Kathrin
  full_name: Tomasek, Kathrin
  id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
  last_name: Tomasek
  orcid: 0000-0003-3768-877X
- first_name: Enrique
  full_name: Balleza, Enrique
  last_name: Balleza
- first_name: Daniel
  full_name: Kiviet, Daniel
  last_name: Kiviet
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Gasper
  full_name: Tkacik, Gasper
  id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
  last_name: Tkacik
  orcid: 0000-0002-6699-1455
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug
    efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. <i>Science</i>.
    2017;356(6335):311-315. doi:<a href="https://doi.org/10.1126/science.aaf4762">10.1126/science.aaf4762</a>
  apa: Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild,
    R., … Guet, C. C. (2017). Biased partitioning of the multidrug efflux pump AcrAB
    TolC underlies long lived phenotypic heterogeneity. <i>Science</i>. American Association
    for the Advancement of Science. <a href="https://doi.org/10.1126/science.aaf4762">https://doi.org/10.1126/science.aaf4762</a>
  chicago: Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza,
    Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning
    of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.”
    <i>Science</i>. American Association for the Advancement of Science, 2017. <a
    href="https://doi.org/10.1126/science.aaf4762">https://doi.org/10.1126/science.aaf4762</a>.
  ieee: T. Bergmiller <i>et al.</i>, “Biased partitioning of the multidrug efflux
    pump AcrAB TolC underlies long lived phenotypic heterogeneity,” <i>Science</i>,
    vol. 356, no. 6335. American Association for the Advancement of Science, pp. 311–315,
    2017.
  ista: Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik
    G, Guet CC. 2017. Biased partitioning of the multidrug efflux pump AcrAB TolC
    underlies long lived phenotypic heterogeneity. Science. 356(6335), 311–315.
  mla: Bergmiller, Tobias, et al. “Biased Partitioning of the Multidrug Efflux Pump
    AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” <i>Science</i>, vol.
    356, no. 6335, American Association for the Advancement of Science, 2017, pp.
    311–15, doi:<a href="https://doi.org/10.1126/science.aaf4762">10.1126/science.aaf4762</a>.
  short: T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild,
    G. Tkačik, C.C. Guet, Science 356 (2017) 311–315.
date_created: 2018-12-11T11:47:48Z
date_published: 2017-04-21T00:00:00Z
date_updated: 2024-02-21T13:49:00Z
day: '21'
department:
- _id: CaGu
- _id: GaTk
- _id: Bio
doi: 10.1126/science.aaf4762
intvolume: '       356'
issue: '6335'
language:
- iso: eng
month: '04'
oa_version: None
page: 311 - 315
project:
- _id: 254E9036-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P28844-B27
  name: Biophysics of information processing in gene regulation
publication: Science
publication_identifier:
  issn:
  - '00368075'
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '7064'
quality_controlled: '1'
related_material:
  record:
  - id: '5560'
    relation: popular_science
    status: public
scopus_import: 1
status: public
title: Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long
  lived phenotypic heterogeneity
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 356
year: '2017'
...
---
_id: '5560'
abstract:
- lang: eng
  text: "This repository contains the data collected for the manuscript \"Biased partitioning
    of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity\".\r\nThe
    data is compressed into a single archive. Within the archive, different folders
    correspond to figures of the main text and the SI of the related publication.\r\nData
    is saved as plain text, with each folder containing a separate readme file describing
    the format. Typically, the data is from fluorescence microscopy measurements of
    single cells growing in a microfluidic \"mother machine\" device, and consists
    of relevant values (primarily arbitrary unit or normalized fluorescence measurements,
    and division times / growth rates) after raw microscopy images have been processed,
    segmented, and their features extracted, as described in the methods section of
    the related publication."
article_processing_charge: No
author:
- first_name: Tobias
  full_name: Bergmiller, Tobias
  id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
  last_name: Bergmiller
  orcid: 0000-0001-5396-4346
- first_name: Anna M
  full_name: Andersson, Anna M
  id: 2B8A40DA-F248-11E8-B48F-1D18A9856A87
  last_name: Andersson
  orcid: 0000-0003-2912-6769
- first_name: Kathrin
  full_name: Tomasek, Kathrin
  id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
  last_name: Tomasek
  orcid: 0000-0003-3768-877X
- first_name: Enrique
  full_name: Balleza, Enrique
  last_name: Balleza
- first_name: Daniel
  full_name: Kiviet, Daniel
  last_name: Kiviet
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Gasper
  full_name: Tkacik, Gasper
  id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
  last_name: Tkacik
  orcid: 0000-0002-6699-1455
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multi-drug
    efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. 2017. doi:<a
    href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>
  apa: Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild,
    R., … Guet, C. C. (2017). Biased partitioning of the multi-drug efflux pump AcrAB-TolC
    underlies long-lived phenotypic heterogeneity. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/AT:ISTA:53">https://doi.org/10.15479/AT:ISTA:53</a>
  chicago: Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza,
    Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning
    of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity.”
    Institute of Science and Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:53">https://doi.org/10.15479/AT:ISTA:53</a>.
  ieee: T. Bergmiller <i>et al.</i>, “Biased partitioning of the multi-drug efflux
    pump AcrAB-TolC underlies long-lived phenotypic heterogeneity.” Institute of Science
    and Technology Austria, 2017.
  ista: Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik
    G, Guet CC. 2017. Biased partitioning of the multi-drug efflux pump AcrAB-TolC
    underlies long-lived phenotypic heterogeneity, Institute of Science and Technology
    Austria, <a href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>.
  mla: Bergmiller, Tobias, et al. <i>Biased Partitioning of the Multi-Drug Efflux
    Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity</i>. Institute of
    Science and Technology Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>.
  short: T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild,
    G. Tkačik, C.C. Guet, (2017).
datarep_id: '53'
date_created: 2018-12-12T12:31:32Z
date_published: 2017-03-10T00:00:00Z
date_updated: 2024-02-21T13:49:00Z
day: '10'
ddc:
- '571'
department:
- _id: CaGu
- _id: GaTk
- _id: Bio
doi: 10.15479/AT:ISTA:53
file:
- access_level: open_access
  checksum: d77859af757ac8025c50c7b12b52eaf3
  content_type: application/zip
  creator: system
  date_created: 2018-12-12T13:02:38Z
  date_updated: 2020-07-14T12:47:03Z
  file_id: '5603'
  file_name: IST-2017-53-v1+1_Data_MDE.zip
  file_size: 6773204
  relation: main_file
file_date_updated: 2020-07-14T12:47:03Z
has_accepted_license: '1'
keyword:
- single cell microscopy
- mother machine microfluidic device
- AcrAB-TolC pump
- multi-drug efflux
- Escherichia coli
month: '03'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '665'
    relation: research_paper
    status: public
status: public
title: Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived
  phenotypic heterogeneity
tmp:
  image: /images/cc_0.png
  legal_code_url: https://creativecommons.org/publicdomain/zero/1.0/legalcode
  name: Creative Commons Public Domain Dedication (CC0 1.0)
  short: CC0 (1.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...