[{"isi":1,"year":"2023","external_id":{"pmid":["37542157"],"isi":["001042544100001"]},"pmid":1,"date_published":"2023-08-04T00:00:00Z","acknowledgement":"We thank Marton Gulyas (ELTE Eötvös University) for development of videomicroscopy experiment manager and image analysis software. Authors are grateful to Gabor Forgacs (University of Missouri) for critical reading of earlier versions of this manuscript as well as to Zsuzsa Akos and Andras Czirok (ELTE Eötvös University) for fruitful discussions. This work was supported by EU FP7, ERC COLLMOT Project No 227878 to TV, the National Research Development and Innovation Fund of Hungary, K119359 and also Project No 2018-1.2.1-NKP-2018-00005 to LN. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 955576. MV was supported by the Ja´nos Bolyai Fellowship of the Hungarian Academy of Sciences.\r\nOpen access funding provided by Eötvös Loránd University.","status":"public","publication":"Communications Biology","_id":"14041","date_updated":"2023-12-13T12:07:33Z","type":"journal_article","article_processing_charge":"Yes","doi":"10.1038/s42003-023-05181-7","publisher":"Springer Nature","quality_controlled":"1","ddc":["570"],"department":[{"_id":"CaHe"},{"_id":"Bio"}],"article_number":"817","file":[{"date_updated":"2023-08-14T07:17:36Z","creator":"dernst","file_size":10181997,"date_created":"2023-08-14T07:17:36Z","file_id":"14045","content_type":"application/pdf","access_level":"open_access","file_name":"2023_CommBiology_Mehes.pdf","success":1,"checksum":"1f9324f736bdbb76426b07736651c4cd","relation":"main_file"}],"month":"08","citation":{"chicago":"Méhes, Elod, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács, László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp J Heisenberg, and Tamás Vicsek. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>.","ista":"Méhes E, Mones E, Varga M, Zsigmond Á, Biri-Kovács B, Nyitray L, Barone V, Krens G, Heisenberg C-PJ, Vicsek T. 2023. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. 6, 817.","mla":"Méhes, Elod, et al. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>, vol. 6, 817, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>.","apa":"Méhes, E., Mones, E., Varga, M., Zsigmond, Á., Biri-Kovács, B., Nyitray, L., … Vicsek, T. (2023). 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>","ama":"Méhes E, Mones E, Varga M, et al. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. 2023;6. doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>","short":"E. Méhes, E. Mones, M. Varga, Á. Zsigmond, B. Biri-Kovács, L. Nyitray, V. Barone, G. Krens, C.-P.J. Heisenberg, T. Vicsek, Communications Biology 6 (2023).","ieee":"E. Méhes <i>et al.</i>, “3D cell segregation geometry and dynamics are governed by tissue surface tension regulation,” <i>Communications Biology</i>, vol. 6. Springer Nature, 2023."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"language":[{"iso":"eng"}],"volume":6,"date_created":"2023-08-13T22:01:13Z","article_type":"original","scopus_import":"1","day":"04","author":[{"first_name":"Elod","last_name":"Méhes","full_name":"Méhes, Elod"},{"first_name":"Enys","full_name":"Mones, Enys","last_name":"Mones"},{"full_name":"Varga, Máté","last_name":"Varga","first_name":"Máté"},{"first_name":"Áron","last_name":"Zsigmond","full_name":"Zsigmond, Áron"},{"first_name":"Beáta","last_name":"Biri-Kovács","full_name":"Biri-Kovács, Beáta"},{"first_name":"László","last_name":"Nyitray","full_name":"Nyitray, László"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","orcid":"0000-0003-2676-3367"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens","first_name":"Gabriel","orcid":"0000-0003-4761-5996"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"},{"first_name":"Tamás","full_name":"Vicsek, Tamás","last_name":"Vicsek"}],"title":"3D cell segregation geometry and dynamics are governed by tissue surface tension regulation","oa_version":"Published Version","file_date_updated":"2023-08-14T07:17:36Z","publication_identifier":{"eissn":["2399-3642"]},"publication_status":"published","has_accepted_license":"1","abstract":[{"lang":"eng","text":"Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated by cell types through controlling cell-cell contact formation by regulating adhesion and actomyosin contractility-based cellular cortical tensions. We use vertebrate tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional heterotypic segregation and developed a quantitative analysis of their dynamics based on 3D time-lapse microscopy. We show that general inhibition of actomyosin contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific inhibition of non-muscle myosin2 activity by overexpression of myosin assembly inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction during aggregation and inverted geometry observed during segregation. The same is observed when we express a constitutively active Rho kinase isoform to ubiquitously keep actomyosin contractility high at cell-cell and cell-medium interfaces and thus overriding the interface-specific regulation of cortical tensions. Tissue surface tension regulation can become an effective tool in tissue engineering."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"intvolume":"         6"},{"day":"14","scopus_import":"1","author":[{"id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","full_name":"Slovakova, Jana","last_name":"Slovakova","first_name":"Jana"},{"last_name":"Sikora","full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K"},{"orcid":"0000-0001-5809-9566","first_name":"Feyza N","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","full_name":"Arslan, Feyza N","last_name":"Arslan"},{"orcid":"0000-0002-5223-3346","first_name":"Silvia","full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","last_name":"Caballero Mancebo"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens","first_name":"Gabriel","orcid":"0000-0003-4761-5996"},{"first_name":"Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter","last_name":"Kaufmann"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"title":"Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells","oa_version":"Published Version","volume":119,"date_created":"2022-02-20T23:01:31Z","article_type":"original","has_accepted_license":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"}],"abstract":[{"text":"Tension of the actomyosin cell cortex plays a key role in determining cell–cell contact growth and size. The level of cortical tension outside of the cell–cell contact, when pulling at the contact edge, scales with the total size to which a cell–cell contact can grow [J.-L. Maître et al., Science 338, 253–256 (2012)]. Here, we show in zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell–cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. After tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell–cell contact size is limited by tension-stabilizing E-cadherin–actin complexes at the contact.","lang":"eng"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"intvolume":"       119","file_date_updated":"2022-02-21T08:45:11Z","publication_status":"published","publication_identifier":{"eissn":["10916490"]},"month":"02","department":[{"_id":"CaHe"},{"_id":"EM-Fac"},{"_id":"Bio"}],"article_number":"e2122030119","file":[{"success":1,"file_name":"2022_PNAS_Slovakova.pdf","access_level":"open_access","content_type":"application/pdf","relation":"main_file","checksum":"d49f83c3580613966f71768ddb9a55a5","file_size":1609678,"date_created":"2022-02-21T08:45:11Z","creator":"dernst","date_updated":"2022-02-21T08:45:11Z","file_id":"10780"}],"oa":1,"language":[{"iso":"eng"}],"citation":{"ieee":"J. Slovakova <i>et al.</i>, “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 8. Proceedings of the National Academy of Sciences, 2022.","short":"J. Slovakova, M.K. Sikora, F.N. Arslan, S. Caballero Mancebo, G. Krens, W. Kaufmann, J. Merrin, C.-P.J. Heisenberg, Proceedings of the National Academy of Sciences of the United States of America 119 (2022).","ama":"Slovakova J, Sikora MK, Arslan FN, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2022;119(8). doi:<a href=\"https://doi.org/10.1073/pnas.2122030119\">10.1073/pnas.2122030119</a>","apa":"Slovakova, J., Sikora, M. K., Arslan, F. N., Caballero Mancebo, S., Krens, G., Kaufmann, W., … Heisenberg, C.-P. J. (2022). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2122030119\">https://doi.org/10.1073/pnas.2122030119</a>","mla":"Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion in Zebrafish Germ-Layer Progenitor Cells.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 8, e2122030119, Proceedings of the National Academy of Sciences, 2022, doi:<a href=\"https://doi.org/10.1073/pnas.2122030119\">10.1073/pnas.2122030119</a>.","chicago":"Slovakova, Jana, Mateusz K Sikora, Feyza N Arslan, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Jack Merrin, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion in Zebrafish Germ-Layer Progenitor Cells.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences, 2022. <a href=\"https://doi.org/10.1073/pnas.2122030119\">https://doi.org/10.1073/pnas.2122030119</a>.","ista":"Slovakova J, Sikora MK, Arslan FN, Caballero Mancebo S, Krens G, Kaufmann W, Merrin J, Heisenberg C-PJ. 2022. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. Proceedings of the National Academy of Sciences of the United States of America. 119(8), e2122030119."},"issue":"8","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","doi":"10.1073/pnas.2122030119","publisher":"Proceedings of the National Academy of Sciences","_id":"10766","date_updated":"2023-08-02T14:26:51Z","type":"journal_article","ddc":["570"],"quality_controlled":"1","isi":1,"year":"2022","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"9750"}]},"external_id":{"isi":["000766926900009"]},"publication":"Proceedings of the National Academy of Sciences of the United States of America","status":"public","project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"grant_number":"187-2013","name":"Modulation of adhesion function in cell-cell contact formation by cortical tension","_id":"2521E28E-B435-11E9-9278-68D0E5697425"}],"ec_funded":1,"acknowledgement":"We thank Guillaume Salbreaux, Silvia Grigolon, Edouard Hannezo, and Vanessa Barone for discussions and comments on the manuscript and Shayan Shamipour and Daniel Capek for help with data analysis. We also thank the Imaging & Optics, Electron Microscopy, and Zebrafish Facility Scientific Service Units at the Institute of Science and Technology Austria (ISTA)Nasser Darwish-Miranda  for continuous support. We acknowledge Hitoshi Morita for the gift of VinculinB-GFP plasmid. This research was supported by an ISTA Fellow Marie-Curie Co-funding of regional, national, and international programmes Grant P_IST_EU01 (to J.S.), European Molecular Biology Organization Long-Term Fellowship Grant, ALTF reference number: 187-2013 (to M.S.), Schroedinger Fellowship J4332-B28 (to M.S.), and European Research Council Advanced Grant (MECSPEC; to C.-P.H.).","date_published":"2022-02-14T00:00:00Z"},{"date_published":"2022-07-11T00:00:00Z","acknowledgement":"This research was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging and Optics, Electron Microscopy, Preclinical and Life Science Facilities. We thank C. Moussion for providing anti-PNAd antibody and D. Critchley for Talin1-floxed mice, and E. Papusheva for providing a custom 3D channel alignment script. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. M.H. was supported by Czech Sciencundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","ec_funded":1,"project":[{"call_identifier":"H2020","grant_number":"724373","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"}],"status":"public","publication":"Nature Immunology","external_id":{"isi":["000822975900002"]},"isi":1,"year":"2022","quality_controlled":"1","ddc":["570"],"page":"1246-1255","type":"journal_article","date_updated":"2023-08-02T06:53:07Z","_id":"9794","publisher":"Springer Nature","doi":"10.1038/s41590-022-01257-4","article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Assen, Frank P, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour, Walter Kaufmann, Tommaso Costanzo, et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” <i>Nature Immunology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41590-022-01257-4\">https://doi.org/10.1038/s41590-022-01257-4</a>.","ista":"Assen FP, Abe J, Hons M, Hauschild R, Shamipour S, Kaufmann W, Costanzo T, Krens G, Brown M, Ludewig B, Hippenmeyer S, Heisenberg C-PJ, Weninger W, Hannezo EB, Luther SA, Stein JV, Sixt MK. 2022. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 23, 1246–1255.","apa":"Assen, F. P., Abe, J., Hons, M., Hauschild, R., Shamipour, S., Kaufmann, W., … Sixt, M. K. (2022). Multitier mechanics control stromal adaptations in swelling lymph nodes. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-022-01257-4\">https://doi.org/10.1038/s41590-022-01257-4</a>","mla":"Assen, Frank P., et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” <i>Nature Immunology</i>, vol. 23, Springer Nature, 2022, pp. 1246–55, doi:<a href=\"https://doi.org/10.1038/s41590-022-01257-4\">10.1038/s41590-022-01257-4</a>.","ama":"Assen FP, Abe J, Hons M, et al. Multitier mechanics control stromal adaptations in swelling lymph nodes. <i>Nature Immunology</i>. 2022;23:1246-1255. doi:<a href=\"https://doi.org/10.1038/s41590-022-01257-4\">10.1038/s41590-022-01257-4</a>","ieee":"F. P. Assen <i>et al.</i>, “Multitier mechanics control stromal adaptations in swelling lymph nodes,” <i>Nature Immunology</i>, vol. 23. Springer Nature, pp. 1246–1255, 2022.","short":"F.P. Assen, J. Abe, M. Hons, R. Hauschild, S. Shamipour, W. Kaufmann, T. Costanzo, G. Krens, M. Brown, B. Ludewig, S. Hippenmeyer, C.-P.J. Heisenberg, W. Weninger, E.B. Hannezo, S.A. Luther, J.V. Stein, M.K. Sixt, Nature Immunology 23 (2022) 1246–1255."},"language":[{"iso":"eng"}],"oa":1,"file":[{"checksum":"628e7b49809f22c75b428842efe70c68","relation":"main_file","content_type":"application/pdf","access_level":"open_access","file_name":"2022_NatureImmunology_Assen.pdf","success":1,"file_id":"11642","creator":"dernst","date_updated":"2022-07-25T07:11:32Z","file_size":11475325,"date_created":"2022-07-25T07:11:32Z"}],"department":[{"_id":"SiHi"},{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"MiSi"}],"month":"07","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"publication_status":"published","file_date_updated":"2022-07-25T07:11:32Z","intvolume":"        23","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular cells that form dedicated niches for immune cell interaction and capsular fibroblasts that build a shell around the organ. Immunological challenge causes LNs to increase more than tenfold in size within a few days. Here, we characterized the biomechanics of LN swelling on the cellular and organ scale. We identified lymphocyte trapping by influx and proliferation as drivers of an outward pressure force, causing fibroblastic reticular cells of the T-zone (TRCs) and their associated conduits to stretch. After an initial phase of relaxation, TRCs sensed the resulting strain through cell matrix adhesions, which coordinated local growth and remodeling of the stromal network. While the expanded TRC network readopted its typical configuration, a massive fibrotic reaction of the organ capsule set in and countered further organ expansion. Thus, different fibroblast populations mechanically control LN swelling in a multitier fashion."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"},{"_id":"LifeSc"}],"has_accepted_license":"1","article_type":"original","date_created":"2021-08-06T09:09:11Z","volume":23,"oa_version":"Published Version","title":"Multitier mechanics control stromal adaptations in swelling lymph nodes","author":[{"last_name":"Assen","full_name":"Assen, Frank P","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3470-6119","first_name":"Frank P"},{"first_name":"Jun","full_name":"Abe, Jun","last_name":"Abe"},{"last_name":"Hons","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348","first_name":"Miroslav"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","last_name":"Shamipour"},{"last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter","first_name":"Walter","orcid":"0000-0001-9735-5315"},{"first_name":"Tommaso","orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","last_name":"Costanzo"},{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markus","last_name":"Brown","full_name":"Brown, Markus","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ludewig","full_name":"Ludewig, Burkhard","first_name":"Burkhard"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","first_name":"Simon"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Weninger, Wolfgang","last_name":"Weninger","first_name":"Wolfgang"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B"},{"first_name":"Sanjiv A.","last_name":"Luther","full_name":"Luther, Sanjiv A."},{"first_name":"Jens V.","full_name":"Stein, Jens V.","last_name":"Stein"},{"last_name":"Sixt","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-4561-241X"}],"day":"11","scopus_import":"1"},{"page":"56-73","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/jmi.13041"}],"publisher":"Wiley","article_processing_charge":"Yes","doi":"10.1111/jmi.13041","type":"journal_article","_id":"9911","date_updated":"2023-08-11T10:30:40Z","status":"public","publication":"Journal of Microscopy","acknowledgement":"We thank https://www.somersault1824.com/somersault18:24 BV (Leuven, Belgium) for help with Figure 1. E. C.-S. was supported by the project PPBI-POCI-01-0145-FEDER-022122, in the scope of Fundação para a Ciência e Tecnologia, Portugal (FCT) National Roadmap of Research Infrastructures. R.N. was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Grant number Ni 451/9-1 - MIAP-Freiburg.","date_published":"2021-08-11T00:00:00Z","external_id":{"isi":["000683702700001"]},"isi":1,"year":"2021","intvolume":"       284","abstract":[{"text":"A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist's experiments, while more than half have even failed to reproduce their own experiments. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g. DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common standards and guidelines for quality assessment and reproducibility. In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper (1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; (2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers and observers of such; (3) outlines the current actions of the QUAREP-LiMi initiative and (4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.","lang":"eng"}],"publication_status":"published","publication_identifier":{"issn":["0022-2720"],"eissn":["1365-2818"]},"oa_version":"Published Version","title":"QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy","scopus_import":"1","day":"11","author":[{"first_name":"Glyn","full_name":"Nelson, Glyn","last_name":"Nelson"},{"full_name":"Boehm, Ulrike","last_name":"Boehm","first_name":"Ulrike"},{"full_name":"Bagley, Steve","last_name":"Bagley","first_name":"Steve"},{"first_name":"Peter","last_name":"Bajcsy","full_name":"Bajcsy, Peter"},{"first_name":"Johanna","last_name":"Bischof","full_name":"Bischof, Johanna"},{"first_name":"Claire M.","last_name":"Brown","full_name":"Brown, Claire M."},{"first_name":"Aurélien","last_name":"Dauphin","full_name":"Dauphin, Aurélien"},{"last_name":"Dobbie","full_name":"Dobbie, Ian M.","first_name":"Ian M."},{"full_name":"Eriksson, John E.","last_name":"Eriksson","first_name":"John E."},{"last_name":"Faklaris","full_name":"Faklaris, Orestis","first_name":"Orestis"},{"full_name":"Fernandez-Rodriguez, Julia","last_name":"Fernandez-Rodriguez","first_name":"Julia"},{"first_name":"Alexia","full_name":"Ferrand, Alexia","last_name":"Ferrand"},{"first_name":"Laurent","last_name":"Gelman","full_name":"Gelman, Laurent"},{"last_name":"Gheisari","full_name":"Gheisari, Ali","first_name":"Ali"},{"full_name":"Hartmann, Hella","last_name":"Hartmann","first_name":"Hella"},{"first_name":"Christian","full_name":"Kukat, Christian","last_name":"Kukat"},{"first_name":"Alex","last_name":"Laude","full_name":"Laude, Alex"},{"first_name":"Miso","full_name":"Mitkovski, Miso","last_name":"Mitkovski"},{"last_name":"Munck","full_name":"Munck, Sebastian","first_name":"Sebastian"},{"last_name":"North","full_name":"North, Alison J.","first_name":"Alison J."},{"full_name":"Rasse, Tobias M.","last_name":"Rasse","first_name":"Tobias M."},{"last_name":"Resch-Genger","full_name":"Resch-Genger, Ute","first_name":"Ute"},{"first_name":"Lucas C.","last_name":"Schuetz","full_name":"Schuetz, Lucas C."},{"last_name":"Seitz","full_name":"Seitz, Arne","first_name":"Arne"},{"last_name":"Strambio-De-Castillia","full_name":"Strambio-De-Castillia, Caterina","first_name":"Caterina"},{"full_name":"Swedlow, Jason R.","last_name":"Swedlow","first_name":"Jason R."},{"first_name":"Ioannis","full_name":"Alexopoulos, Ioannis","last_name":"Alexopoulos"},{"first_name":"Karin","last_name":"Aumayr","full_name":"Aumayr, Karin"},{"first_name":"Sergiy","last_name":"Avilov","full_name":"Avilov, Sergiy"},{"last_name":"Bakker","full_name":"Bakker, Gert Jan","first_name":"Gert Jan"},{"first_name":"Rodrigo R.","last_name":"Bammann","full_name":"Bammann, Rodrigo R."},{"first_name":"Andrea","full_name":"Bassi, Andrea","last_name":"Bassi"},{"full_name":"Beckert, Hannes","last_name":"Beckert","first_name":"Hannes"},{"first_name":"Sebastian","last_name":"Beer","full_name":"Beer, Sebastian"},{"first_name":"Yury","last_name":"Belyaev","full_name":"Belyaev, Yury"},{"first_name":"Jakob","last_name":"Bierwagen","full_name":"Bierwagen, Jakob"},{"full_name":"Birngruber, Konstantin A.","last_name":"Birngruber","first_name":"Konstantin A."},{"last_name":"Bosch","full_name":"Bosch, Manel","first_name":"Manel"},{"last_name":"Breitlow","full_name":"Breitlow, Juergen","first_name":"Juergen"},{"first_name":"Lisa A.","last_name":"Cameron","full_name":"Cameron, Lisa A."},{"first_name":"Joe","last_name":"Chalfoun","full_name":"Chalfoun, Joe"},{"last_name":"Chambers","full_name":"Chambers, James J.","first_name":"James J."},{"first_name":"Chieh Li","full_name":"Chen, Chieh Li","last_name":"Chen"},{"first_name":"Eduardo","last_name":"Conde-Sousa","full_name":"Conde-Sousa, Eduardo"},{"first_name":"Alexander D.","last_name":"Corbett","full_name":"Corbett, Alexander D."},{"first_name":"Fabrice P.","last_name":"Cordelieres","full_name":"Cordelieres, Fabrice P."},{"full_name":"Nery, Elaine Del","last_name":"Nery","first_name":"Elaine Del"},{"full_name":"Dietzel, Ralf","last_name":"Dietzel","first_name":"Ralf"},{"full_name":"Eismann, Frank","last_name":"Eismann","first_name":"Frank"},{"full_name":"Fazeli, Elnaz","last_name":"Fazeli","first_name":"Elnaz"},{"last_name":"Felscher","full_name":"Felscher, Andreas","first_name":"Andreas"},{"first_name":"Hans","full_name":"Fried, Hans","last_name":"Fried"},{"full_name":"Gaudreault, Nathalie","last_name":"Gaudreault","first_name":"Nathalie"},{"full_name":"Goh, Wah Ing","last_name":"Goh","first_name":"Wah Ing"},{"first_name":"Thomas","full_name":"Guilbert, Thomas","last_name":"Guilbert"},{"full_name":"Hadleigh, Roland","last_name":"Hadleigh","first_name":"Roland"},{"first_name":"Peter","last_name":"Hemmerich","full_name":"Hemmerich, Peter"},{"first_name":"Gerhard A.","last_name":"Holst","full_name":"Holst, Gerhard A."},{"last_name":"Itano","full_name":"Itano, Michelle S.","first_name":"Michelle S."},{"first_name":"Claudia B.","last_name":"Jaffe","full_name":"Jaffe, Claudia B."},{"first_name":"Helena K.","full_name":"Jambor, Helena K.","last_name":"Jambor"},{"first_name":"Stuart C.","full_name":"Jarvis, Stuart C.","last_name":"Jarvis"},{"full_name":"Keppler, Antje","last_name":"Keppler","first_name":"Antje"},{"first_name":"David","last_name":"Kirchenbuechler","full_name":"Kirchenbuechler, David"},{"last_name":"Kirchner","full_name":"Kirchner, Marcel","first_name":"Marcel"},{"first_name":"Norio","last_name":"Kobayashi","full_name":"Kobayashi, Norio"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens"},{"first_name":"Susanne","last_name":"Kunis","full_name":"Kunis, Susanne"},{"full_name":"Lacoste, Judith","last_name":"Lacoste","first_name":"Judith"},{"last_name":"Marcello","full_name":"Marcello, Marco","first_name":"Marco"},{"first_name":"Gabriel G.","full_name":"Martins, Gabriel G.","last_name":"Martins"},{"first_name":"Daniel J.","full_name":"Metcalf, Daniel J.","last_name":"Metcalf"},{"full_name":"Mitchell, Claire A.","last_name":"Mitchell","first_name":"Claire A."},{"first_name":"Joshua","full_name":"Moore, Joshua","last_name":"Moore"},{"full_name":"Mueller, Tobias","last_name":"Mueller","first_name":"Tobias"},{"last_name":"Nelson","full_name":"Nelson, Michael S.","first_name":"Michael S."},{"first_name":"Stephen","full_name":"Ogg, Stephen","last_name":"Ogg"},{"first_name":"Shuichi","last_name":"Onami","full_name":"Onami, Shuichi"},{"first_name":"Alexandra L.","last_name":"Palmer","full_name":"Palmer, Alexandra L."},{"first_name":"Perrine","full_name":"Paul-Gilloteaux, Perrine","last_name":"Paul-Gilloteaux"},{"first_name":"Jaime A.","full_name":"Pimentel, Jaime A.","last_name":"Pimentel"},{"last_name":"Plantard","full_name":"Plantard, Laure","first_name":"Laure"},{"full_name":"Podder, Santosh","last_name":"Podder","first_name":"Santosh"},{"full_name":"Rexhepaj, Elton","last_name":"Rexhepaj","first_name":"Elton"},{"full_name":"Royon, Arnaud","last_name":"Royon","first_name":"Arnaud"},{"first_name":"Markku A.","full_name":"Saari, Markku A.","last_name":"Saari"},{"full_name":"Schapman, Damien","last_name":"Schapman","first_name":"Damien"},{"full_name":"Schoonderwoert, Vincent","last_name":"Schoonderwoert","first_name":"Vincent"},{"last_name":"Schroth-Diez","full_name":"Schroth-Diez, Britta","first_name":"Britta"},{"last_name":"Schwartz","full_name":"Schwartz, Stanley","first_name":"Stanley"},{"first_name":"Michael","full_name":"Shaw, Michael","last_name":"Shaw"},{"last_name":"Spitaler","full_name":"Spitaler, Martin","first_name":"Martin"},{"full_name":"Stoeckl, Martin T.","last_name":"Stoeckl","first_name":"Martin T."},{"full_name":"Sudar, Damir","last_name":"Sudar","first_name":"Damir"},{"full_name":"Teillon, Jeremie","last_name":"Teillon","first_name":"Jeremie"},{"first_name":"Stefan","last_name":"Terjung","full_name":"Terjung, Stefan"},{"full_name":"Thuenauer, Roland","last_name":"Thuenauer","first_name":"Roland"},{"full_name":"Wilms, Christian D.","last_name":"Wilms","first_name":"Christian D."},{"first_name":"Graham D.","last_name":"Wright","full_name":"Wright, Graham D."},{"first_name":"Roland","full_name":"Nitschke, Roland","last_name":"Nitschke"}],"date_created":"2021-08-15T22:01:29Z","article_type":"original","volume":284,"language":[{"iso":"eng"}],"oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Nelson G et al. 2021. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of Microscopy. 284(1), 56–73.","chicago":"Nelson, Glyn, Ulrike Boehm, Steve Bagley, Peter Bajcsy, Johanna Bischof, Claire M. Brown, Aurélien Dauphin, et al. “QUAREP-LiMi: A Community-Driven Initiative to Establish Guidelines for Quality Assessment and Reproducibility for Instruments and Images in Light Microscopy.” <i>Journal of Microscopy</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/jmi.13041\">https://doi.org/10.1111/jmi.13041</a>.","apa":"Nelson, G., Boehm, U., Bagley, S., Bajcsy, P., Bischof, J., Brown, C. M., … Nitschke, R. (2021). QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. <i>Journal of Microscopy</i>. Wiley. <a href=\"https://doi.org/10.1111/jmi.13041\">https://doi.org/10.1111/jmi.13041</a>","mla":"Nelson, Glyn, et al. “QUAREP-LiMi: A Community-Driven Initiative to Establish Guidelines for Quality Assessment and Reproducibility for Instruments and Images in Light Microscopy.” <i>Journal of Microscopy</i>, vol. 284, no. 1, Wiley, 2021, pp. 56–73, doi:<a href=\"https://doi.org/10.1111/jmi.13041\">10.1111/jmi.13041</a>.","ama":"Nelson G, Boehm U, Bagley S, et al. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. <i>Journal of Microscopy</i>. 2021;284(1):56-73. doi:<a href=\"https://doi.org/10.1111/jmi.13041\">10.1111/jmi.13041</a>","ieee":"G. Nelson <i>et al.</i>, “QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy,” <i>Journal of Microscopy</i>, vol. 284, no. 1. Wiley, pp. 56–73, 2021.","short":"G. Nelson, U. Boehm, S. Bagley, P. Bajcsy, J. Bischof, C.M. Brown, A. Dauphin, I.M. Dobbie, J.E. Eriksson, O. Faklaris, J. Fernandez-Rodriguez, A. Ferrand, L. Gelman, A. Gheisari, H. Hartmann, C. Kukat, A. Laude, M. Mitkovski, S. Munck, A.J. North, T.M. Rasse, U. Resch-Genger, L.C. Schuetz, A. Seitz, C. Strambio-De-Castillia, J.R. Swedlow, I. Alexopoulos, K. Aumayr, S. Avilov, G.J. Bakker, R.R. Bammann, A. Bassi, H. Beckert, S. Beer, Y. Belyaev, J. Bierwagen, K.A. Birngruber, M. Bosch, J. Breitlow, L.A. Cameron, J. Chalfoun, J.J. Chambers, C.L. Chen, E. Conde-Sousa, A.D. Corbett, F.P. Cordelieres, E.D. Nery, R. Dietzel, F. Eismann, E. Fazeli, A. Felscher, H. Fried, N. Gaudreault, W.I. Goh, T. Guilbert, R. Hadleigh, P. Hemmerich, G.A. Holst, M.S. Itano, C.B. Jaffe, H.K. Jambor, S.C. Jarvis, A. Keppler, D. Kirchenbuechler, M. Kirchner, N. Kobayashi, G. Krens, S. Kunis, J. Lacoste, M. Marcello, G.G. Martins, D.J. Metcalf, C.A. Mitchell, J. Moore, T. Mueller, M.S. Nelson, S. Ogg, S. Onami, A.L. Palmer, P. Paul-Gilloteaux, J.A. Pimentel, L. Plantard, S. Podder, E. Rexhepaj, A. Royon, M.A. Saari, D. Schapman, V. Schoonderwoert, B. Schroth-Diez, S. Schwartz, M. Shaw, M. Spitaler, M.T. Stoeckl, D. Sudar, J. Teillon, S. Terjung, R. Thuenauer, C.D. Wilms, G.D. Wright, R. Nitschke, Journal of Microscopy 284 (2021) 56–73."},"issue":"1","month":"08","department":[{"_id":"Bio"}]},{"department":[{"_id":"CaHe"},{"_id":"EM-Fac"},{"_id":"Bio"}],"year":"2020","month":"11","related_material":{"record":[{"relation":"later_version","status":"public","id":"10766"},{"relation":"dissertation_contains","status":"public","id":"9623"}]},"citation":{"mla":"Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, 2020, doi:<a href=\"https://doi.org/10.1101/2020.11.20.391284\">10.1101/2020.11.20.391284</a>.","apa":"Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W., Huljev, K., &#38; Heisenberg, C.-P. J. (2020). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. <i>bioRxiv</i>. Cold Spring Harbor Laboratory. <a href=\"https://doi.org/10.1101/2020.11.20.391284\">https://doi.org/10.1101/2020.11.20.391284</a>","chicago":"Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, 2020. <a href=\"https://doi.org/10.1101/2020.11.20.391284\">https://doi.org/10.1101/2020.11.20.391284</a>.","ista":"Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K, Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv, <a href=\"https://doi.org/10.1101/2020.11.20.391284\">10.1101/2020.11.20.391284</a>.","short":"J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K. Huljev, C.-P.J. Heisenberg, BioRxiv (2020).","ieee":"J. Slovakova <i>et al.</i>, “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory, 2020.","ama":"Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. <i>bioRxiv</i>. 2020. doi:<a href=\"https://doi.org/10.1101/2020.11.20.391284\">10.1101/2020.11.20.391284</a>"},"ec_funded":1,"acknowledgement":"We would like to thank Edouard Hannezo for discussions, Shayan Shami Pour and Daniel Capek for help with data analysis, Vanessa Barone and other members of the Heisenberg laboratory for thoughtful discussions and comments on the manuscript. We also thank Jack Merrin for preparing the microwells, and the Scientific Service Units at IST Austria, specifically Bioimaging and Electron Microscopy, and the Zebrafish Facility for continuous support. We acknowledge Hitoshi Morita for the kind gift of VinculinB-GFP plasmid. This research was supported by an ERC Advanced Grant (MECSPEC) to C.-P.H, EMBO Long Term grant (ALTF 187-2013) to M.S and IST Fellow Marie-Curie COFUND No. P_IST_EU01 to J.S.","date_published":"2020-11-20T00:00:00Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","oa":1,"project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020"},{"_id":"2521E28E-B435-11E9-9278-68D0E5697425","grant_number":"187-2013","name":"Modulation of adhesion function in cell-cell contact formation by cortical tension"}],"language":[{"iso":"eng"}],"publication":"bioRxiv","status":"public","date_updated":"2024-03-25T23:30:10Z","_id":"9750","type":"preprint","date_created":"2021-07-29T11:29:50Z","author":[{"last_name":"Slovakova","full_name":"Slovakova, Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","first_name":"Jana"},{"first_name":"Mateusz K","last_name":"Sikora","full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-5223-3346","first_name":"Silvia","last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens"},{"full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","orcid":"0000-0001-9735-5315","first_name":"Walter"},{"first_name":"Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","full_name":"Huljev, Karla","last_name":"Huljev"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"doi":"10.1101/2020.11.20.391284","article_processing_charge":"No","day":"20","title":"Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion","oa_version":"Preprint","publisher":"Cold Spring Harbor Laboratory","main_file_link":[{"url":"https://doi.org/10.1101/2020.11.20.391284","open_access":"1"}],"publication_status":"published","page":"41","abstract":[{"text":"Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase, and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension stabilizing E-cadherin-actin complexes at the contact.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"SSU"}]},{"page":"331 - 346","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2018.04.002"}],"publisher":"Elsevier","article_processing_charge":"No","doi":"10.1016/j.devcel.2018.04.002","type":"journal_article","_id":"308","date_updated":"2023-09-11T13:22:13Z","publication":"Developmental Cell","status":"public","project":[{"_id":"253B6E48-B435-11E9-9278-68D0E5697425","name":"Drosophila TNFa´s Funktion in Immunzellen","grant_number":"P29638","call_identifier":"FWF"},{"call_identifier":"FP7","grant_number":"334077","name":"Investigating the role of transporters in invasive migration through junctions","_id":"2536F660-B435-11E9-9278-68D0E5697425"}],"date_published":"2018-05-07T00:00:00Z","ec_funded":1,"pmid":1,"related_material":{"link":[{"url":"https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/","description":"News on IST Homepage","relation":"press_release"}]},"external_id":{"pmid":["29738712"],"isi":["000432461400009"]},"year":"2018","isi":1,"acknowledged_ssus":[{"_id":"SSU"}],"intvolume":"        45","abstract":[{"lang":"eng","text":"Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo."}],"publication_status":"published","title":"Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration","oa_version":"Published Version","scopus_import":"1","day":"07","author":[{"full_name":"Ratheesh, Aparna","id":"2F064CFE-F248-11E8-B48F-1D18A9856A87","last_name":"Ratheesh","orcid":"0000-0001-7190-0776","first_name":"Aparna"},{"id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","full_name":"Biebl, Julia","last_name":"Biebl","first_name":"Julia"},{"first_name":"Michael","last_name":"Smutny","full_name":"Smutny, Michael"},{"full_name":"Veselá, Jana","id":"433253EE-F248-11E8-B48F-1D18A9856A87","last_name":"Veselá","first_name":"Jana"},{"last_name":"Papusheva","full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina"},{"full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","orcid":"0000-0003-4761-5996","first_name":"Gabriel"},{"orcid":"0000-0001-9735-5315","first_name":"Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter"},{"last_name":"György","full_name":"György, Attila","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1819-198X","first_name":"Attila"},{"last_name":"Casano","full_name":"Casano, Alessandra M","id":"3DBA3F4E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6009-6804","first_name":"Alessandra M"},{"full_name":"Siekhaus, Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus","first_name":"Daria E","orcid":"0000-0001-8323-8353"}],"date_created":"2018-12-11T11:45:44Z","article_type":"original","volume":45,"language":[{"iso":"eng"}],"oa":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ieee":"A. Ratheesh <i>et al.</i>, “Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration,” <i>Developmental Cell</i>, vol. 45, no. 3. Elsevier, pp. 331–346, 2018.","short":"A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W. Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018) 331–346.","ama":"Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>. 2018;45(3):331-346. doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">10.1016/j.devcel.2018.04.002</a>","apa":"Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G., … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">https://doi.org/10.1016/j.devcel.2018.04.002</a>","mla":"Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>, vol. 45, no. 3, Elsevier, 2018, pp. 331–46, doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">10.1016/j.devcel.2018.04.002</a>.","chicago":"Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano, and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” <i>Developmental Cell</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.devcel.2018.04.002\">https://doi.org/10.1016/j.devcel.2018.04.002</a>.","ista":"Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W, György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 45(3), 331–346."},"issue":"3","month":"05","department":[{"_id":"DaSi"},{"_id":"CaHe"},{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"MiSi"}]},{"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"10083"}]},"year":"2018","publist_id":"7381","project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","grant_number":"665385","call_identifier":"H2020"}],"publication":"Bio-protocol","status":"public","acknowledgement":"This protocol was adapted from Fendrych et al., 2016. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian Science Fund (FWF) [M 2128-B21]. ","date_published":"2018-01-05T00:00:00Z","ec_funded":1,"publisher":"Bio-protocol","doi":"10.21769/BioProtoc.2685","article_processing_charge":"No","type":"journal_article","date_updated":"2024-10-29T10:22:43Z","_id":"442","ddc":["576","581"],"quality_controlled":"1","month":"01","file":[{"checksum":"6644ba698206eda32b0abf09128e63e3","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf","file_id":"5299","date_updated":"2020-07-14T12:46:29Z","creator":"system","file_size":11352389,"date_created":"2018-12-12T10:17:43Z"}],"department":[{"_id":"JiFr"},{"_id":"Bio"}],"pubrep_id":"970","language":[{"iso":"eng"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"1","citation":{"ama":"Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-protocol</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>","short":"L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).","ieee":"L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” <i>Bio-protocol</i>, vol. 8, no. 1. Bio-protocol, 2018.","ista":"Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 8(1).","chicago":"Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>. Bio-protocol, 2018. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>.","mla":"Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>, vol. 8, no. 1, Bio-protocol, 2018, doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>.","apa":"Li, L., Krens, G., Fendrych, M., &#38; Friml, J. (2018). Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-Protocol</i>. Bio-protocol. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>"},"title":"Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls","oa_version":"Published Version","author":[{"last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin","orcid":"0000-0002-5607-272X","first_name":"Lanxin"},{"full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","orcid":"0000-0003-4761-5996","first_name":"Gabriel"},{"first_name":"Matyas","orcid":"0000-0002-9767-8699","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml"}],"day":"05","article_type":"original","date_created":"2018-12-11T11:46:30Z","volume":8,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.","lang":"eng"}],"intvolume":"         8","has_accepted_license":"1","publication_status":"published","publication_identifier":{"eissn":["2331-8325"]},"file_date_updated":"2020-07-14T12:46:29Z"},{"acknowledged_ssus":[{"_id":"PreCl"}],"intvolume":"        40","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during “doming,” when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.","lang":"eng"}],"has_accepted_license":"1","file_date_updated":"2018-12-12T10:10:57Z","publication_status":"published","publication_identifier":{"issn":["15345807"]},"title":"The physical basis of coordinated tissue spreading in zebrafish gastrulation","oa_version":"Published Version","scopus_import":"1","day":"27","author":[{"first_name":"Hitoshi","full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","last_name":"Morita"},{"last_name":"Grigolon","full_name":"Grigolon, Silvia","first_name":"Silvia"},{"first_name":"Martin","last_name":"Bock","full_name":"Bock, Martin"},{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens"},{"first_name":"Guillaume","last_name":"Salbreux","full_name":"Salbreux, Guillaume"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"date_created":"2018-12-11T11:49:58Z","volume":40,"pubrep_id":"869","language":[{"iso":"eng"}],"oa":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ama":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. The physical basis of coordinated tissue spreading in zebrafish gastrulation. <i>Developmental Cell</i>. 2017;40(4):354-366. doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">10.1016/j.devcel.2017.01.010</a>","ieee":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, and C.-P. J. Heisenberg, “The physical basis of coordinated tissue spreading in zebrafish gastrulation,” <i>Developmental Cell</i>, vol. 40, no. 4. Cell Press, pp. 354–366, 2017.","short":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, C.-P.J. Heisenberg, Developmental Cell 40 (2017) 354–366.","chicago":"Morita, Hitoshi, Silvia Grigolon, Martin Bock, Gabriel Krens, Guillaume Salbreux, and Carl-Philipp J Heisenberg. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” <i>Developmental Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">https://doi.org/10.1016/j.devcel.2017.01.010</a>.","ista":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. 2017. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 40(4), 354–366.","apa":"Morita, H., Grigolon, S., Bock, M., Krens, G., Salbreux, G., &#38; Heisenberg, C.-P. J. (2017). The physical basis of coordinated tissue spreading in zebrafish gastrulation. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">https://doi.org/10.1016/j.devcel.2017.01.010</a>","mla":"Morita, Hitoshi, et al. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” <i>Developmental Cell</i>, vol. 40, no. 4, Cell Press, 2017, pp. 354–66, doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">10.1016/j.devcel.2017.01.010</a>."},"issue":"4","month":"02","file":[{"content_type":"application/pdf","access_level":"open_access","file_name":"IST-2017-869-v1+1_1-s2.0-S1534580717300370-main.pdf","relation":"main_file","date_updated":"2018-12-12T10:10:57Z","creator":"system","file_size":6866187,"date_created":"2018-12-12T10:10:57Z","file_id":"4849"}],"department":[{"_id":"CaHe"}],"ddc":["572","597"],"page":"354 - 366","quality_controlled":"1","publisher":"Cell Press","article_processing_charge":"No","doi":"10.1016/j.devcel.2017.01.010","type":"journal_article","_id":"1067","date_updated":"2023-09-20T12:06:27Z","status":"public","publication":"Developmental Cell","project":[{"_id":"2524F500-B435-11E9-9278-68D0E5697425","grant_number":"201439","name":"Developing High-Throughput Bioassays for Human Cancers in Zebrafish","call_identifier":"FP7"}],"date_published":"2017-02-27T00:00:00Z","ec_funded":1,"external_id":{"isi":["000395368300007"]},"isi":1,"year":"2017","publist_id":"6320"},{"month":"05","department":[{"_id":"Bio"},{"_id":"CaHe"}],"file":[{"file_id":"6905","file_size":8194516,"date_created":"2019-09-24T06:56:22Z","date_updated":"2020-07-14T12:47:39Z","creator":"dernst","relation":"main_file","checksum":"bc25125fb664706cdf180e061429f91d","file_name":"2017_Development_Krens.pdf","content_type":"application/pdf","access_level":"open_access"}],"oa":1,"language":[{"iso":"eng"}],"issue":"10","citation":{"apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., &#38; Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>.","chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>. Company of Biologists, 2017. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>.","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806.","ieee":"G. Krens <i>et al.</i>, “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” <i>Development</i>, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.","short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806.","ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. 2017;144(10):1798-1806. doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens"},{"first_name":"Jim","full_name":"Veldhuis, Jim","last_name":"Veldhuis"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","orcid":"0000-0003-2676-3367"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","last_name":"Capek","first_name":"Daniel","orcid":"0000-0001-5199-9940"},{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","last_name":"Maître","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474"},{"full_name":"Brodland, Wayne","last_name":"Brodland","first_name":"Wayne"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":1,"day":"15","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","oa_version":"Published Version","volume":144,"article_type":"original","date_created":"2018-12-11T11:47:52Z","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"intvolume":"       144","abstract":[{"lang":"eng","text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation."}],"publication_status":"published","publication_identifier":{"issn":["09501991"]},"file_date_updated":"2020-07-14T12:47:39Z","year":"2017","external_id":{"pmid":["28512197"]},"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"961"},{"status":"public","relation":"dissertation_contains","id":"50"}]},"publist_id":"7047","status":"public","publication":"Development","pmid":1,"date_published":"2017-05-15T00:00:00Z","doi":"10.1242/dev.144964","article_processing_charge":"No","publisher":"Company of Biologists","date_updated":"2024-03-25T23:30:13Z","_id":"676","type":"journal_article","page":"1798 - 1806","ddc":["570"],"quality_controlled":"1"},{"language":[{"iso":"eng"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ista":"Barone V, Lang M, Krens G, Pradhan S, Shamipour S, Sako K, Sikora MK, Guet CC, Heisenberg C-PJ. 2017. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 43(2), 198–211.","chicago":"Barone, Vanessa, Moritz Lang, Gabriel Krens, Saurabh Pradhan, Shayan Shamipour, Keisuke Sako, Mateusz K Sikora, Calin C Guet, and Carl-Philipp J Heisenberg. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” <i>Developmental Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">https://doi.org/10.1016/j.devcel.2017.09.014</a>.","apa":"Barone, V., Lang, M., Krens, G., Pradhan, S., Shamipour, S., Sako, K., … Heisenberg, C.-P. J. (2017). An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">https://doi.org/10.1016/j.devcel.2017.09.014</a>","mla":"Barone, Vanessa, et al. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” <i>Developmental Cell</i>, vol. 43, no. 2, Cell Press, 2017, pp. 198–211, doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">10.1016/j.devcel.2017.09.014</a>.","ama":"Barone V, Lang M, Krens G, et al. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. <i>Developmental Cell</i>. 2017;43(2):198-211. doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">10.1016/j.devcel.2017.09.014</a>","ieee":"V. Barone <i>et al.</i>, “An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate,” <i>Developmental Cell</i>, vol. 43, no. 2. Cell Press, pp. 198–211, 2017.","short":"V. Barone, M. Lang, G. Krens, S. Pradhan, S. Shamipour, K. Sako, M.K. Sikora, C.C. Guet, C.-P.J. Heisenberg, Developmental Cell 43 (2017) 198–211."},"issue":"2","month":"10","department":[{"_id":"CaHe"},{"_id":"CaGu"},{"_id":"GaTk"}],"intvolume":"        43","abstract":[{"lang":"eng","text":"Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo."}],"publication_identifier":{"issn":["15345807"]},"publication_status":"published","title":"An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate","oa_version":"None","scopus_import":"1","day":"23","author":[{"first_name":"Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","last_name":"Barone"},{"last_name":"Lang","id":"29E0800A-F248-11E8-B48F-1D18A9856A87","full_name":"Lang, Moritz","first_name":"Moritz"},{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Pradhan, Saurabh","last_name":"Pradhan","first_name":"Saurabh"},{"first_name":"Shayan","full_name":"Shamipour, Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","last_name":"Shamipour"},{"orcid":"0000-0002-6453-8075","first_name":"Keisuke","full_name":"Sako, Keisuke","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","last_name":"Sako"},{"first_name":"Mateusz K","full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora"},{"full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","orcid":"0000-0001-6220-2052","first_name":"Calin C"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"date_created":"2018-12-11T11:48:13Z","volume":43,"status":"public","publication":"Developmental Cell","project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","grant_number":"I2058","name":"Cell segregation in gastrulation: the role of cell fate specification","_id":"252DD2A6-B435-11E9-9278-68D0E5697425"}],"date_published":"2017-10-23T00:00:00Z","ec_funded":1,"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"961"},{"relation":"dissertation_contains","status":"public","id":"8350"}]},"external_id":{"isi":["000413443700011"]},"year":"2017","isi":1,"publist_id":"6934","page":"198 - 211","quality_controlled":"1","publisher":"Cell Press","article_processing_charge":"No","doi":"10.1016/j.devcel.2017.09.014","type":"journal_article","_id":"735","date_updated":"2024-03-25T23:30:21Z"},{"type":"journal_article","_id":"1817","date_updated":"2021-01-12T06:53:23Z","publisher":"Nature Publishing Group","doi":"10.1038/nature14215","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720436/"}],"page":"217 - 221","publist_id":"5289","external_id":{"pmid":["25778702"]},"year":"2015","date_published":"2015-03-16T00:00:00Z","pmid":1,"status":"public","publication":"Nature","date_created":"2018-12-11T11:54:10Z","volume":521,"title":"YAP is essential for tissue tension to ensure vertebrate 3D body shape","oa_version":"Submitted Version","day":"16","scopus_import":1,"author":[{"full_name":"Porazinski, Sean","last_name":"Porazinski","first_name":"Sean"},{"first_name":"Huijia","full_name":"Wang, Huijia","last_name":"Wang"},{"first_name":"Yoichi","full_name":"Asaoka, Yoichi","last_name":"Asaoka"},{"last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin","first_name":"Martin"},{"full_name":"Miyamoto, Tatsuo","last_name":"Miyamoto","first_name":"Tatsuo"},{"first_name":"Hitoshi","full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","last_name":"Morita"},{"full_name":"Hata, Shoji","last_name":"Hata","first_name":"Shoji"},{"first_name":"Takashi","last_name":"Sasaki","full_name":"Sasaki, Takashi"},{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Osada, Yumi","last_name":"Osada","first_name":"Yumi"},{"full_name":"Asaka, Satoshi","last_name":"Asaka","first_name":"Satoshi"},{"first_name":"Akihiro","last_name":"Momoi","full_name":"Momoi, Akihiro"},{"full_name":"Linton, Sarah","last_name":"Linton","first_name":"Sarah"},{"full_name":"Miesfeld, Joel","last_name":"Miesfeld","first_name":"Joel"},{"full_name":"Link, Brian","last_name":"Link","first_name":"Brian"},{"first_name":"Takeshi","last_name":"Senga","full_name":"Senga, Takeshi"},{"first_name":"Atahualpa","full_name":"Castillo Morales, Atahualpa","last_name":"Castillo Morales"},{"last_name":"Urrutia","full_name":"Urrutia, Araxi","first_name":"Araxi"},{"last_name":"Shimizu","full_name":"Shimizu, Nobuyoshi","first_name":"Nobuyoshi"},{"first_name":"Hideaki","full_name":"Nagase, Hideaki","last_name":"Nagase"},{"full_name":"Matsuura, Shinya","last_name":"Matsuura","first_name":"Shinya"},{"first_name":"Stefan","full_name":"Bagby, Stefan","last_name":"Bagby"},{"last_name":"Kondoh","full_name":"Kondoh, Hisato","first_name":"Hisato"},{"first_name":"Hiroshi","full_name":"Nishina, Hiroshi","last_name":"Nishina"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Furutani Seiki, Makoto","last_name":"Furutani Seiki","first_name":"Makoto"}],"publication_status":"published","abstract":[{"lang":"eng","text":"Vertebrates have a unique 3D body shape in which correct tissue and organ shape and alignment are essential for function. For example, vision requires the lens to be centred in the eye cup which must in turn be correctly positioned in the head. Tissue morphogenesis depends on force generation, force transmission through the tissue, and response of tissues and extracellular matrix to force. Although a century ago D'Arcy Thompson postulated that terrestrial animal body shapes are conditioned by gravity, there has been no animal model directly demonstrating how the aforementioned mechano-morphogenetic processes are coordinated to generate a body shape that withstands gravity. Here we report a unique medaka fish (Oryzias latipes) mutant, hirame (hir), which is sensitive to deformation by gravity. hir embryos display a markedly flattened body caused by mutation of YAP, a nuclear executor of Hippo signalling that regulates organ size. We show that actomyosin-mediated tissue tension is reduced in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to body flattening. By analysing YAP function in 3D spheroids of human cells, we identify the Rho GTPase activating protein ARHGAP18 as an effector of YAP in controlling tissue tension. Together, these findings reveal a previously unrecognised function of YAP in regulating tissue shape and alignment required for proper 3D body shape. Understanding this morphogenetic function of YAP could facilitate the use of embryonic stem cells to generate complex organs requiring correct alignment of multiple tissues. "}],"intvolume":"       521","department":[{"_id":"CaHe"}],"month":"03","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Porazinski, S., Wang, H., Asaoka, Y., Behrndt, M., Miyamoto, T., Morita, H., … Furutani Seiki, M. (2015). YAP is essential for tissue tension to ensure vertebrate 3D body shape. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature14215\">https://doi.org/10.1038/nature14215</a>","mla":"Porazinski, Sean, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” <i>Nature</i>, vol. 521, no. 7551, Nature Publishing Group, 2015, pp. 217–21, doi:<a href=\"https://doi.org/10.1038/nature14215\">10.1038/nature14215</a>.","chicago":"Porazinski, Sean, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” <i>Nature</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nature14215\">https://doi.org/10.1038/nature14215</a>.","ista":"Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki T, Krens G, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld J, Link B, Senga T, Castillo Morales A, Urrutia A, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh H, Nishina H, Heisenberg C-PJ, Furutani Seiki M. 2015. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 521(7551), 217–221.","ieee":"S. Porazinski <i>et al.</i>, “YAP is essential for tissue tension to ensure vertebrate 3D body shape,” <i>Nature</i>, vol. 521, no. 7551. Nature Publishing Group, pp. 217–221, 2015.","short":"S. Porazinski, H. Wang, Y. Asaoka, M. Behrndt, T. Miyamoto, H. Morita, S. Hata, T. Sasaki, G. Krens, Y. Osada, S. Asaka, A. Momoi, S. Linton, J. Miesfeld, B. Link, T. Senga, A. Castillo Morales, A. Urrutia, N. Shimizu, H. Nagase, S. Matsuura, S. Bagby, H. Kondoh, H. Nishina, C.-P.J. Heisenberg, M. Furutani Seiki, Nature 521 (2015) 217–221.","ama":"Porazinski S, Wang H, Asaoka Y, et al. YAP is essential for tissue tension to ensure vertebrate 3D body shape. <i>Nature</i>. 2015;521(7551):217-221. doi:<a href=\"https://doi.org/10.1038/nature14215\">10.1038/nature14215</a>"},"issue":"7551","language":[{"iso":"eng"}],"oa":1},{"intvolume":"        29","page":"147 - 150","publication_status":"published","quality_controlled":"1","publisher":"Éditions Médicales et Scientifiques","title":"Cell adhesion mechanics of zebrafish gastrulation","oa_version":"None","day":"01","scopus_import":1,"author":[{"orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","last_name":"Maître"},{"full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux","first_name":"Hélène"},{"full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","orcid":"0000-0003-4761-5996","first_name":"Gabriel"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"},{"full_name":"Julicher, Frank","last_name":"Julicher","first_name":"Frank"},{"last_name":"Paluch","full_name":"Paluch, Ewa","first_name":"Ewa"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"doi":"10.1051/medsci/2013292011","date_created":"2018-12-11T12:00:08Z","type":"journal_article","volume":29,"_id":"2884","date_updated":"2021-01-12T07:00:28Z","language":[{"iso":"eng"}],"publication":"Medecine Sciences","status":"public","project":[{"name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo","grant_number":"HE_3231/6-1","_id":"252064B8-B435-11E9-9278-68D0E5697425"},{"_id":"2527D5CC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","grant_number":"I 812-B12"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2013-02-01T00:00:00Z","citation":{"ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. 29(2), 147–150.","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques, 2013. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>","mla":"Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>, vol. 29, no. 2, Éditions Médicales et Scientifiques, 2013, pp. 147–50, doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. 2013;29(2):147-150. doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>","ieee":"J.-L. Maître <i>et al.</i>, “Cell adhesion mechanics of zebrafish gastrulation,” <i>Medecine Sciences</i>, vol. 29, no. 2. Éditions Médicales et Scientifiques, pp. 147–150, 2013.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150."},"issue":"2","month":"02","year":"2013","publist_id":"3877","department":[{"_id":"CaHe"}]},{"month":"10","year":"2012","publist_id":"3777","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"publication":"Science","status":"public","date_published":"2012-10-12T00:00:00Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","issue":"6104","citation":{"ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 338(6104), 253–256.","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>","mla":"Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 253–56, doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. 2012;338(6104):253-256. doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>","ieee":"J.-L. Maître <i>et al.</i>, “Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells,” <i>Science</i>, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 253–256, 2012.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Science 338 (2012) 253–256."},"oa_version":"None","publisher":"American Association for the Advancement of Science","title":"Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells","doi":"10.1126/science.1225399","author":[{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","last_name":"Maître","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474"},{"full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux","first_name":"Hélène"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"full_name":"Julicher, Frank","last_name":"Julicher","first_name":"Frank"},{"first_name":"Ewa","last_name":"Paluch","full_name":"Paluch, Ewa"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"scopus_import":1,"day":"12","type":"journal_article","date_created":"2018-12-11T12:00:31Z","date_updated":"2021-01-12T07:40:00Z","_id":"2951","volume":338,"intvolume":"       338","abstract":[{"text":"Differential cell adhesion and cortex tension are thought to drive cell sorting by controlling cell-cell contact formation. Here, we show that cell adhesion and cortex tension have different mechanical functions in controlling progenitor cell-cell contact formation and sorting during zebrafish gastrulation. Cortex tension controls cell-cell contact expansion by modulating interfacial tension at the contact. By contrast, adhesion has little direct function in contact expansion, but instead is needed to mechanically couple the cortices of adhering cells at their contacts, allowing cortex tension to control contact expansion. The coupling function of adhesion is mediated by E-cadherin and limited by the mechanical anchoring of E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation.","lang":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"page":"253 - 256","publication_status":"published","quality_controlled":"1"},{"type":"journal_article","date_updated":"2021-01-12T07:43:00Z","_id":"3368","publisher":"National Academy of Sciences","doi":"10.1073/pnas.1010767108","quality_controlled":"1","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024655","open_access":"1"}],"page":"E9 - E10","publist_id":"3244","external_id":{"pmid":["21212360"]},"year":"2011","date_published":"2011-01-18T00:00:00Z","pmid":1,"status":"public","publication":"PNAS","date_created":"2018-12-11T12:02:56Z","volume":108,"title":"Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants","oa_version":"Submitted Version","author":[{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens"},{"id":"260FD49C-E911-11E9-B5EA-D9538404589B","full_name":"Möllmert, Stephanie","last_name":"Möllmert","first_name":"Stephanie"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"scopus_import":1,"day":"18","publication_status":"published","intvolume":"       108","abstract":[{"text":"Tissue surface tension (TST) is an important mechanical property influencing cell sorting and tissue envelopment. The study by Manning et al. (1) reported on a mathematical model describing TST on the basis of the balance between adhesive and tensile properties of the constituent cells. The model predicts that, in high-adhesion cell aggregates, surface cells will be stretched to maintain the same area of cell–cell contact as interior bulk cells, resulting in an elongated and flattened cell shape. The authors (1) observed flat and elongated cells at the surface of high-adhesion zebrafish germ-layer explants, which they argue are undifferentiated stretched germ-layer progenitor cells, and they use this observation as a validation of their model.","lang":"eng"}],"department":[{"_id":"CaHe"}],"month":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"3","citation":{"short":"G. Krens, S. Möllmert, C.-P.J. Heisenberg, PNAS 108 (2011) E9–E10.","ieee":"G. Krens, S. Möllmert, and C.-P. J. Heisenberg, “Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants,” <i>PNAS</i>, vol. 108, no. 3. National Academy of Sciences, pp. E9–E10, 2011.","ama":"Krens G, Möllmert S, Heisenberg C-PJ. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. 2011;108(3):E9-E10. doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>","mla":"Krens, Gabriel, et al. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>, vol. 108, no. 3, National Academy of Sciences, 2011, pp. E9–10, doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>.","apa":"Krens, G., Möllmert, S., &#38; Heisenberg, C.-P. J. (2011). Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>","chicago":"Krens, Gabriel, Stephanie Möllmert, and Carl-Philipp J Heisenberg. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>. National Academy of Sciences, 2011. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>.","ista":"Krens G, Möllmert S, Heisenberg C-PJ. 2011. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. 108(3), E9–E10."},"language":[{"iso":"eng"}],"oa":1},{"publication":"Forces and Tension in Development","language":[{"iso":"eng"}],"status":"public","date_published":"2011-01-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ama":"Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed. <i>Forces and Tension in Development</i>. Vol 95. Elsevier; 2011:189-213. doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>","short":"G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension in Development, Elsevier, 2011, pp. 189–213.","ieee":"G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in <i>Forces and Tension in Development</i>, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp. 189–213.","ista":"Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.","chicago":"Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.” In <i>Forces and Tension in Development</i>, edited by Michel Labouesse, 95:189–213. Elsevier, 2011. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>.","mla":"Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.” <i>Forces and Tension in Development</i>, edited by Michel Labouesse, vol. 95, Elsevier, 2011, pp. 189–213, doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>.","apa":"Krens, G., &#38; Heisenberg, C.-P. J. (2011). Cell sorting in development. In M. Labouesse (Ed.), <i>Forces and Tension in Development</i> (Vol. 95, pp. 189–213). Elsevier. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>"},"month":"01","year":"2011","publist_id":"2436","department":[{"_id":"CaHe"}],"abstract":[{"lang":"eng","text":"During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active maintenance of their segregated state. Because of its biological importance and apparent resemblance to fluid segregation in physics, cell sorting was extensively studied by both biologists and physicists over the last decades. Different theories were developed that try to explain cell sorting on the basis of the physical properties of the constituent cells. However, only recently the molecular and cellular mechanisms that control the physical properties driving cell sorting, have begun to be unraveled. In this review, we will provide an overview of different cell-sorting processes in development and discuss how these processes can be explained by the different sorting theories, and how these theories in turn can be connected to the molecular and cellular mechanisms driving these processes."}],"intvolume":"        95","page":"189 - 213","publication_status":"published","quality_controlled":"1","editor":[{"first_name":"Michel","full_name":"Labouesse, Michel","last_name":"Labouesse"}],"oa_version":"None","title":"Cell sorting in development","publisher":"Elsevier","author":[{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"doi":"10.1016/B978-0-12-385065-2.00006-2","article_processing_charge":"No","day":"01","scopus_import":"1","alternative_title":["Current Topics in Developmental Biology"],"type":"book_chapter","date_created":"2018-12-11T12:05:11Z","date_updated":"2021-01-12T07:52:13Z","volume":95,"_id":"3791"},{"issue":"2","citation":{"ieee":"A. Klopper, G. Krens, S. Grill, and C.-P. J. Heisenberg, “Finite-size corrections to scaling behavior in sorted cell aggregates,” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>, vol. 33, no. 2. Springer, pp. 99–103, 2010.","short":"A. Klopper, G. Krens, S. Grill, C.-P.J. Heisenberg, The European Physical Journal E: Soft Matter and Biological Physics 33 (2010) 99–103.","ama":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. Finite-size corrections to scaling behavior in sorted cell aggregates. <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. 2010;33(2):99-103. doi:<a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">10.1140/epje/i2010-10642-y</a>","apa":"Klopper, A., Krens, G., Grill, S., &#38; Heisenberg, C.-P. J. (2010). Finite-size corrections to scaling behavior in sorted cell aggregates. <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. Springer. <a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">https://doi.org/10.1140/epje/i2010-10642-y</a>","mla":"Klopper, Abigail, et al. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>, vol. 33, no. 2, Springer, 2010, pp. 99–103, doi:<a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">10.1140/epje/i2010-10642-y</a>.","chicago":"Klopper, Abigail, Gabriel Krens, Stephan Grill, and Carl-Philipp J Heisenberg. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. Springer, 2010. <a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">https://doi.org/10.1140/epje/i2010-10642-y</a>.","ista":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. 2010. Finite-size corrections to scaling behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter and Biological Physics. 33(2), 99–103."},"date_published":"2010-09-18T00:00:00Z","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","status":"public","language":[{"iso":"eng"}],"publication":"The European Physical Journal E: Soft Matter and Biological Physics","department":[{"_id":"CaHe"}],"publist_id":"2439","year":"2010","month":"09","publication_status":"published","page":"99 - 103","intvolume":"        33","abstract":[{"lang":"eng","text":"Cell sorting is a widespread phenomenon pivotal to the early development of multicellular organisms. In vitro cell sorting studies have been instrumental in revealing the cellular properties driving this process. However, these studies have as yet been limited to two-dimensional analysis of three-dimensional cell sorting events. Here we describe a method to record the sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, and quantitatively analyze their sorting behavior using an order parameter related to heterotypic interface length. We investigate the cell population size dependence of sorted aggregates and find that the germ layer progenitor cells engulfed in the final configuration display a relationship between total interfacial length and system size according to a simple geometrical argument, subject to a finite-size effect."}],"date_updated":"2021-01-12T07:52:12Z","volume":33,"_id":"3788","type":"journal_article","date_created":"2018-12-11T12:05:10Z","doi":"10.1140/epje/i2010-10642-y","author":[{"last_name":"Klopper","full_name":"Klopper, Abigail","first_name":"Abigail"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens"},{"full_name":"Grill, Stephan","last_name":"Grill","first_name":"Stephan"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"scopus_import":1,"day":"18","oa_version":"None","title":"Finite-size corrections to scaling behavior in sorted cell aggregates","publisher":"Springer"}]