[{"file_date_updated":"2022-08-08T07:09:58Z","publication":"Proceedings of the National Academy of Sciences of the United States of America","issue":"31","status":"public","intvolume":"       119","type":"journal_article","day":"25","date_created":"2022-08-07T22:01:57Z","file":[{"file_name":"2022_PNAS_Abualia.pdf","file_size":3092330,"date_created":"2022-08-08T07:09:58Z","checksum":"6e97dedc281247fc3fe238a209f14af0","date_updated":"2022-08-08T07:09:58Z","access_level":"open_access","success":1,"content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"11744"}],"department":[{"_id":"EvBe"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Proceedings of the National Academy of Sciences","date_published":"2022-07-25T00:00:00Z","article_type":"original","month":"07","quality_controlled":"1","project":[{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF"}],"oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"We acknowledge Hana Semeradova, Juan Carlos Montesinos, Nicola Cavallari, Marc¸al Gallem\u0003ı, Kaori Tabata, Andrej Hurn\u0003y, and Sascha Waidmann for sharing materials; and Marina Borges Osorio for critical reading of the manuscript. Work in the E. Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to K.O., R.A., and E. Benkova. We acknowledge the Bioimaging Facility and Life Science Facilities of the Institute of Science\r\nand Technology Austria. We give sincere thanks to Hana Martınkova and Petra Amakorova for their help with cytokinin analyses. This work was funded by the Czech Science Foundation (Project No. 19-00973S).","publication_identifier":{"eissn":["1091-6490"]},"pmid":1,"_id":"11734","article_processing_charge":"No","oa":1,"volume":119,"date_updated":"2023-08-03T12:39:29Z","author":[{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","first_name":"Rashed","full_name":"Abualia, Rashed","last_name":"Abualia","orcid":"0000-0002-9357-9415"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","last_name":"Ötvös","full_name":"Ötvös, Krisztina","orcid":"0000-0002-5503-4983","first_name":"Krisztina"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"first_name":"Eleonore","full_name":"Bouguyon, Eleonore","last_name":"Bouguyon"},{"id":"a24c7829-16e8-11ed-8527-c4d36ffb7539","full_name":"Domanegg, Kevin","last_name":"Domanegg","orcid":"0000-0002-1215-4264","first_name":"Kevin"},{"first_name":"Anne","last_name":"Krapp","full_name":"Krapp, Anne"},{"first_name":"Philip","full_name":"Nacry, Philip","last_name":"Nacry"},{"first_name":"Alain","full_name":"Gojon, Alain","last_name":"Gojon"},{"first_name":"Benoit","last_name":"Lacombe","full_name":"Lacombe, Benoit"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739"}],"abstract":[{"lang":"eng","text":"Mineral nutrition is one of the key environmental factors determining plant development and growth. Nitrate is the major form of macronutrient nitrogen that plants take up from the soil. Fluctuating availability or deficiency of this element severely limits plant growth and negatively affects crop production in the agricultural system. To cope with the heterogeneity of nitrate distribution in soil, plants evolved a complex regulatory mechanism that allows rapid adjustment of physiological and developmental processes to the status of this nutrient. The root, as a major exploitation organ that controls the uptake of nitrate to the plant body, acts as a regulatory hub that, according to nitrate availability, coordinates the growth and development of other plant organs. Here, we identified a regulatory framework, where cytokinin response factors (CRFs) play a central role as a molecular readout of the nitrate status in roots to guide shoot adaptive developmental response. We show that nitrate-driven activation of NLP7, a master regulator of nitrate response in plants, fine tunes biosynthesis of cytokinin in roots and its translocation to shoots where it enhances expression of CRFs. CRFs, through direct transcriptional regulation of PIN auxin transporters, promote the flow of auxin and thereby stimulate the development of shoot organs."}],"citation":{"mla":"Abualia, Rashed, et al. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31, e2122460119, Proceedings of the National Academy of Sciences, 2022, doi:<a href=\"https://doi.org/10.1073/pnas.2122460119\">10.1073/pnas.2122460119</a>.","ama":"Abualia R, Ötvös K, Novák O, et al. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2022;119(31). doi:<a href=\"https://doi.org/10.1073/pnas.2122460119\">10.1073/pnas.2122460119</a>","ista":"Abualia R, Ötvös K, Novák O, Bouguyon E, Domanegg K, Krapp A, Nacry P, Gojon A, Lacombe B, Benková E. 2022. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. Proceedings of the National Academy of Sciences of the United States of America. 119(31), e2122460119.","short":"R. Abualia, K. Ötvös, O. Novák, E. Bouguyon, K. Domanegg, A. Krapp, P. Nacry, A. Gojon, B. Lacombe, E. Benková, Proceedings of the National Academy of Sciences of the United States of America 119 (2022).","ieee":"R. Abualia <i>et al.</i>, “Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31. Proceedings of the National Academy of Sciences, 2022.","apa":"Abualia, R., Ötvös, K., Novák, O., Bouguyon, E., Domanegg, K., Krapp, A., … Benková, E. (2022). Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2122460119\">https://doi.org/10.1073/pnas.2122460119</a>","chicago":"Abualia, Rashed, Krisztina Ötvös, Ondřej Novák, Eleonore Bouguyon, Kevin Domanegg, Anne Krapp, Philip Nacry, Alain Gojon, Benoit Lacombe, and Eva Benková. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences, 2022. <a href=\"https://doi.org/10.1073/pnas.2122460119\">https://doi.org/10.1073/pnas.2122460119</a>."},"publication_status":"published","ddc":["570"],"isi":1,"article_number":"e2122460119","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"title":"Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses","external_id":{"pmid":["35878040"],"isi":["000881496900007"]},"doi":"10.1073/pnas.2122460119","year":"2022","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}]},{"date_created":"2021-01-17T23:01:12Z","file":[{"file_id":"9110","creator":"dernst","content_type":"application/pdf","relation":"main_file","success":1,"date_updated":"2021-02-11T12:28:29Z","access_level":"open_access","date_created":"2021-02-11T12:28:29Z","checksum":"dc55c900f3b061d6c2790b8813d759a3","file_name":"2021_Embo_Otvos.pdf","file_size":2358617}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"publisher":"Embo Press","scopus_import":"1","date_published":"2021-02-01T00:00:00Z","article_type":"original","month":"02","file_date_updated":"2021-02-11T12:28:29Z","issue":"3","publication":"EMBO Journal","status":"public","intvolume":"        40","type":"journal_article","day":"01","ddc":["580"],"related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/"}],"record":[{"relation":"dissertation_contains","id":"10303","status":"public"}]},"isi":1,"article_number":"e106862","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"external_id":{"isi":["000604645600001"],"pmid":[" 33399250"]},"title":"Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport","acknowledged_ssus":[{"_id":"Bio"}],"doi":"10.15252/embj.2020106862","year":"2021","acknowledgement":"We acknowledge Gergely Molnar for critical reading of the manuscript, Alexander Johnson for language editing and Yulija Salanenka for technical assistance. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654 to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.). M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund (FWF): I03630 to J.F","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","project":[{"name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I 1774-B16"},{"_id":"2685A872-B435-11E9-9278-68D0E5697425","name":"Hormonal regulation of plant adaptive responses to environmental signals"},{"grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","_id":"9010","pmid":1,"publication_identifier":{"issn":["02614189"],"eissn":["14602075"]},"volume":40,"date_updated":"2024-03-25T23:30:22Z","oa":1,"article_processing_charge":"Yes (via OA deal)","author":[{"first_name":"Krisztina","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Marconi, Marco","last_name":"Marconi","first_name":"Marco"},{"full_name":"Vega, Andrea","last_name":"Vega","first_name":"Andrea"},{"first_name":"Jose","last_name":"O’Brien","full_name":"O’Brien, Jose"},{"first_name":"Alexander J","orcid":"0000-0002-2739-8843","last_name":"Johnson","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Abualia, Rashed","last_name":"Abualia","orcid":"0000-0002-9357-9415","first_name":"Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Livio","full_name":"Antonielli, Livio","last_name":"Antonielli"},{"full_name":"Montesinos López, Juan C","last_name":"Montesinos López","orcid":"0000-0001-9179-6099","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","first_name":"Yuzhou","orcid":"0000-0003-2627-6956","last_name":"Zhang","full_name":"Zhang, Yuzhou"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","last_name":"Tan","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","full_name":"Cuesta, Candela"},{"first_name":"Christina","last_name":"Artner","full_name":"Artner, Christina","id":"45DF286A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eleonore","last_name":"Bouguyon","full_name":"Bouguyon, Eleonore"},{"last_name":"Gojon","full_name":"Gojon, Alain","first_name":"Alain"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Rodrigo A.","last_name":"Gutiérrez","full_name":"Gutiérrez, Rodrigo A."},{"orcid":"0000-0001-7263-0560","full_name":"Wabnik, Krzysztof T","last_name":"Wabnik","first_name":"Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"lang":"eng","text":"Availability of the essential macronutrient nitrogen in soil plays a critical role in plant growth, development, and impacts agricultural productivity. Plants have evolved different strategies for sensing and responding to heterogeneous nitrogen distribution. Modulation of root system architecture, including primary root growth and branching, is among the most essential plant adaptions to ensure adequate nitrogen acquisition. However, the immediate molecular pathways coordinating the adjustment of root growth in response to distinct nitrogen sources, such as nitrate or ammonium, are poorly understood. Here, we show that growth as manifested by cell division and elongation is synchronized by coordinated auxin flux between two adjacent outer tissue layers of the root. This coordination is achieved by nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization and thereby regulating auxin flow between adjacent tissues. A dynamic computer model based on our experimental data successfully recapitulates experimental observations. Our study provides mechanistic insights broadening our understanding of root growth mechanisms in dynamic environments."}],"publication_status":"published","citation":{"chicago":"Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>.","ieee":"K. Ötvös <i>et al.</i>, “Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport,” <i>EMBO Journal</i>, vol. 40, no. 3. Embo Press, 2021.","apa":"Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R., … Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>","short":"K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli, J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A. Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).","ista":"Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L, Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A, Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. EMBO Journal. 40(3), e106862.","ama":"Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. 2021;40(3). doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>","mla":"Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>, vol. 40, no. 3, e106862, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>."}},{"article_processing_charge":"No","volume":22,"oa":1,"date_updated":"2023-08-08T13:09:58Z","quality_controlled":"1","oa_version":"Published Version","acknowledgement":"This research was supported by a postdoctoral fellowship of the Carl Tryggers Foundation (to K.Ö.) and by grants from Vetenskapsrådet (Nr.: 621-2004-2921 to L.B.) and VINNOVA (to L.B. and S.R.).\r\nWe thank Frederic Berger, Hidehiro Fukaki, Malcolm Bennett, Claudia Köhler, Jiri Friml for providing pRBR1::RBR1-RFP, ssl2-1, slr-1, pPKL::PKL-GFP seeds and the DR5 expressing vector, respectively. Authors are grateful to Hayashi Kenichiro for providing the auxinol compound and to Rishi Bhalerao for stimulating discussions. The technical help of Adeline Rigal and Thomas Vain with the auxinol experiments is much appreciated.","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"eissn":["1422-0067"],"issn":["1661-6596"]},"_id":"9332","citation":{"ieee":"K. Ötvös <i>et al.</i>, “Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 8. MDPI, 2021.","apa":"Ötvös, K., Miskolczi, P., Marhavý, P., Cruz-Ramírez, A., Benková, E., Robert, S., &#38; Bakó, L. (2021). Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22083862\">https://doi.org/10.3390/ijms22083862</a>","chicago":"Ötvös, Krisztina, Pál Miskolczi, Peter Marhavý, Alfredo Cruz-Ramírez, Eva Benková, Stéphanie Robert, and László Bakó. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22083862\">https://doi.org/10.3390/ijms22083862</a>.","ama":"Ötvös K, Miskolczi P, Marhavý P, et al. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. <i>International Journal of Molecular Sciences</i>. 2021;22(8). doi:<a href=\"https://doi.org/10.3390/ijms22083862\">10.3390/ijms22083862</a>","mla":"Ötvös, Krisztina, et al. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 8, 3862, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22083862\">10.3390/ijms22083862</a>.","short":"K. Ötvös, P. Miskolczi, P. Marhavý, A. Cruz-Ramírez, E. Benková, S. Robert, L. Bakó, International Journal of Molecular Sciences 22 (2021).","ista":"Ötvös K, Miskolczi P, Marhavý P, Cruz-Ramírez A, Benková E, Robert S, Bakó L. 2021. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. International Journal of Molecular Sciences. 22(8), 3862."},"publication_status":"published","author":[{"orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös","first_name":"Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Miskolczi","full_name":"Miskolczi, Pál","first_name":"Pál"},{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavý","full_name":"Marhavý, Peter","orcid":"0000-0001-5227-5741","first_name":"Peter"},{"first_name":"Alfredo","full_name":"Cruz-Ramírez, Alfredo","last_name":"Cruz-Ramírez"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva"},{"last_name":"Robert","full_name":"Robert, Stéphanie","first_name":"Stéphanie"},{"full_name":"Bakó, László","last_name":"Bakó","first_name":"László"}],"abstract":[{"text":"Lateral root (LR) formation is an example of a plant post-embryonic organogenesis event. LRs are issued from non-dividing cells entering consecutive steps of formative divisions, proliferation and elongation. The chromatin remodeling protein PICKLE (PKL) negatively regulates auxin-mediated LR formation through a mechanism that is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED 1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity. Since LBD16 function is required for the formative division of LR founder cells, repression mediated by the PKL–RBR1 complex negatively regulates formative division and LR formation. Inhibition of LR formation by PKL–RBR1 is counteracted by auxin, indicating that, in addition to auxin-mediated transcriptional responses, the fine-tuned process of LR formation is also controlled at the chromatin level in an auxin-signaling dependent manner.","lang":"eng"}],"article_number":"3862","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"doi":"10.3390/ijms22083862","year":"2021","title":"Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis","external_id":{"isi":["000644394800001"]},"file_date_updated":"2021-04-19T10:54:55Z","publication":"International Journal of Molecular Sciences","issue":"8","type":"journal_article","day":"08","status":"public","intvolume":"        22","department":[{"_id":"EvBe"}],"has_accepted_license":"1","file":[{"success":1,"creator":"dernst","file_id":"9342","content_type":"application/pdf","relation":"main_file","date_created":"2021-04-19T10:54:55Z","checksum":"26ada2531ad1f9c01a1664de0431f1fe","file_name":"2021_JourMolecularScience_Oetvoes.pdf","file_size":2769717,"date_updated":"2021-04-19T10:54:55Z","access_level":"open_access"}],"date_created":"2021-04-18T22:01:41Z","date_published":"2021-04-08T00:00:00Z","article_type":"original","month":"04","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"MDPI"},{"intvolume":"        22","status":"public","day":"06","type":"journal_article","issue":"9","publication":"EMBO Reports","file_date_updated":"2021-10-05T13:36:42Z","publisher":"Wiley","scopus_import":"1","language":[{"iso":"eng"}],"month":"09","date_published":"2021-09-06T00:00:00Z","article_type":"original","file":[{"date_created":"2021-10-05T13:36:42Z","checksum":"750de03dc3b715c37090126c1548ba13","file_name":"2021_EmboR_Vega.pdf","file_size":3144854,"date_updated":"2021-10-05T13:36:42Z","access_level":"open_access","success":1,"creator":"cchlebak","file_id":"10090","content_type":"application/pdf","relation":"main_file"}],"date_created":"2021-08-15T22:01:30Z","has_accepted_license":"1","department":[{"_id":"EvBe"},{"_id":"GradSch"}],"abstract":[{"lang":"eng","text":"Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate."}],"author":[{"first_name":"Andrea","last_name":"Vega","full_name":"Vega, Andrea"},{"first_name":"Isabel","last_name":"Fredes","full_name":"Fredes, Isabel"},{"first_name":"José","full_name":"O’Brien, José","last_name":"O’Brien"},{"first_name":"Zhouxin","full_name":"Shen, Zhouxin","last_name":"Shen"},{"full_name":"Ötvös, Krisztina","last_name":"Ötvös","orcid":"0000-0002-5503-4983","first_name":"Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","first_name":"Rashed","full_name":"Abualia, Rashed","last_name":"Abualia","orcid":"0000-0002-9357-9415"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Steven P.","full_name":"Briggs, Steven P.","last_name":"Briggs"},{"first_name":"Rodrigo A.","full_name":"Gutiérrez, Rodrigo A.","last_name":"Gutiérrez"}],"publication_status":"published","citation":{"short":"A. Vega, I. Fredes, J. O’Brien, Z. Shen, K. Ötvös, R. Abualia, E. Benková, S.P. Briggs, R.A. Gutiérrez, EMBO Reports 22 (2021).","ista":"Vega A, Fredes I, O’Brien J, Shen Z, Ötvös K, Abualia R, Benková E, Briggs SP, Gutiérrez RA. 2021. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. EMBO Reports. 22(9), e51813.","mla":"Vega, Andrea, et al. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>, vol. 22, no. 9, e51813, Wiley, 2021, doi:<a href=\"https://doi.org/10.15252/embr.202051813\">10.15252/embr.202051813</a>.","ama":"Vega A, Fredes I, O’Brien J, et al. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. 2021;22(9). doi:<a href=\"https://doi.org/10.15252/embr.202051813\">10.15252/embr.202051813</a>","chicago":"Vega, Andrea, Isabel Fredes, José O’Brien, Zhouxin Shen, Krisztina Ötvös, Rashed Abualia, Eva Benková, Steven P. Briggs, and Rodrigo A. Gutiérrez. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>. Wiley, 2021. <a href=\"https://doi.org/10.15252/embr.202051813\">https://doi.org/10.15252/embr.202051813</a>.","ieee":"A. Vega <i>et al.</i>, “Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture,” <i>EMBO Reports</i>, vol. 22, no. 9. Wiley, 2021.","apa":"Vega, A., Fredes, I., O’Brien, J., Shen, Z., Ötvös, K., Abualia, R., … Gutiérrez, R. A. (2021). Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. Wiley. <a href=\"https://doi.org/10.15252/embr.202051813\">https://doi.org/10.15252/embr.202051813</a>"},"pmid":1,"_id":"9913","publication_identifier":{"issn":["1469-221X"],"eissn":["1469-3178"]},"acknowledgement":"This work was supported by ANID—Millennium Science Initiative Program—ICN17_022, Fondo de Desarrollo de Areas Prioritarias (FONDAP) Center for Genome Regulation (15090007), ANID—Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1180759 (to RAG) and 1171631 (to AV). We would like to thank Unidad de Microscopía Avanzada UC (UMA UC).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","quality_controlled":"1","volume":22,"date_updated":"2024-03-25T23:30:22Z","oa":1,"article_processing_charge":"Yes","external_id":{"pmid":["34357701 "],"isi":["000681754200001"]},"title":"Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture","doi":"10.15252/embr.202051813","year":"2021","related_material":{"record":[{"status":"public","id":"10303","relation":"dissertation_contains"}]},"ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"e51813"},{"ddc":["570"],"article_number":"2170","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"title":"Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance","external_id":{"pmid":["32358503"],"isi":["000531425900012"]},"ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2020","doi":"10.1038/s41467-020-15895-5","acknowledgement":"We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing published material, Lesia Rodriguez Solovey for technical assistance. This work was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B., the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","project":[{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","grant_number":"I 1774-B16"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"}],"quality_controlled":"1","oa_version":"Published Version","pmid":1,"_id":"7805","publication_identifier":{"eissn":["20411723"]},"oa":1,"volume":11,"date_updated":"2023-08-21T06:21:56Z","article_processing_charge":"No","author":[{"id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Andrej","orcid":"0000-0003-3638-1426","last_name":"Hurny","full_name":"Hurny, Andrej"},{"last_name":"Cuesta","full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola","last_name":"Cavallari","first_name":"Nicola"},{"first_name":"Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jerome","full_name":"Duclercq, Jerome","last_name":"Duclercq"},{"first_name":"Ladislav","last_name":"Dokládal","full_name":"Dokládal, Ladislav"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C","last_name":"Montesinos López","first_name":"Juan C"},{"id":"460C6802-F248-11E8-B48F-1D18A9856A87","first_name":"Marçal","orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","last_name":"Gallemi"},{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","full_name":"Semeradova, Hana","last_name":"Semeradova","first_name":"Hana"},{"id":"A0385D1A-9376-11EA-A47D-9862C5E3AB22","last_name":"Rauter","full_name":"Rauter, Thomas","first_name":"Thomas"},{"full_name":"Stenzel, Irene","last_name":"Stenzel","first_name":"Irene"},{"first_name":"Geert","last_name":"Persiau","full_name":"Persiau, Geert"},{"first_name":"Freia","last_name":"Benade","full_name":"Benade, Freia"},{"first_name":"Rishikesh","last_name":"Bhalearo","full_name":"Bhalearo, Rishikesh"},{"last_name":"Sýkorová","full_name":"Sýkorová, Eva","first_name":"Eva"},{"last_name":"Gorzsás","full_name":"Gorzsás, András","first_name":"András"},{"last_name":"Sechet","full_name":"Sechet, Julien","first_name":"Julien"},{"last_name":"Mouille","full_name":"Mouille, Gregory","first_name":"Gregory"},{"last_name":"Heilmann","full_name":"Heilmann, Ingo","first_name":"Ingo"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"last_name":"Ludwig-Müller","full_name":"Ludwig-Müller, Jutta","first_name":"Jutta"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens.","lang":"eng"}],"publication_status":"published","citation":{"mla":"Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>, vol. 11, 2170, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>.","ama":"Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>","ista":"Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.","short":"A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C. Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau, F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann, G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).","apa":"Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L., … Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>","ieee":"A. Hurny <i>et al.</i>, “Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","chicago":"Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>."},"file":[{"success":1,"file_id":"8614","creator":"dernst","relation":"main_file","content_type":"application/pdf","checksum":"2cba327c9e9416d75cb96be54b0fb441","date_created":"2020-10-06T07:47:53Z","file_size":4743576,"file_name":"2020_NatureComm_Hurny.pdf","access_level":"open_access","date_updated":"2020-10-06T07:47:53Z"}],"date_created":"2020-05-10T22:00:48Z","department":[{"_id":"EvBe"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"publisher":"Springer Nature","scopus_import":"1","date_published":"2020-05-01T00:00:00Z","article_type":"original","month":"05","file_date_updated":"2020-10-06T07:47:53Z","publication":"Nature Communications","status":"public","intvolume":"        11","type":"journal_article","day":"01"},{"publication":"The Embo Journal","issue":"17","file_date_updated":"2020-12-02T09:13:23Z","intvolume":"        39","status":"public","day":"01","type":"journal_article","date_created":"2020-07-21T09:08:38Z","file":[{"date_updated":"2020-12-02T09:13:23Z","access_level":"open_access","file_name":"2020_EMBO_Montesinos.pdf","file_size":3497156,"date_created":"2020-12-02T09:13:23Z","checksum":"43d2b36598708e6ab05c69074e191d57","content_type":"application/pdf","relation":"main_file","file_id":"8827","creator":"dernst","success":1}],"has_accepted_license":"1","department":[{"_id":"MiSi"},{"_id":"EvBe"}],"scopus_import":"1","publisher":"Embo Press","language":[{"iso":"eng"}],"month":"09","date_published":"2020-09-01T00:00:00Z","article_type":"original","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"pmid":1,"_id":"8142","oa_version":"Published Version","project":[{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016"},{"grant_number":"I 1774-B16","call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development"}],"quality_controlled":"1","acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"Yes (via OA deal)","oa":1,"date_updated":"2023-09-05T13:05:47Z","volume":39,"abstract":[{"lang":"eng","text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells."}],"author":[{"orcid":"0000-0001-9179-6099","last_name":"Montesinos López","full_name":"Montesinos López, Juan C","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Abuzeineh, A","last_name":"Abuzeineh","first_name":"A"},{"first_name":"Aglaja","orcid":"0000-0002-2187-6656","last_name":"Kopf","full_name":"Kopf, Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87"},{"id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba","orcid":"0000-0002-1009-9652","last_name":"Juanes Garcia","full_name":"Juanes Garcia, Alba"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös","first_name":"Krisztina"},{"first_name":"J","last_name":"Petrášek","full_name":"Petrášek, J"},{"first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"}],"citation":{"ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238.","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).","mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>.","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>","ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020."},"publication_status":"published","ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"e104238","title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","external_id":{"pmid":["32667089"],"isi":["000548311800001"]},"doi":"10.15252/embj.2019104238","year":"2020","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}]},{"title":"Spatiotemporal mechanisms of root branching","external_id":{"isi":["000404880400013"],"pmid":["28391060"]},"pubrep_id":"1017","year":"2017","doi":"10.1016/j.gde.2017.03.010","ddc":["575"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"isi":1,"abstract":[{"text":"The fundamental tasks of the root system are, besides anchoring, mediating interactions between plant and soil and providing the plant with water and nutrients. The architecture of the root system is controlled by endogenous mechanisms that constantly integrate environmental signals, such as availability of nutrients and water. Extremely important for efficient soil exploitation and survival under less favorable conditions is the developmental flexibility of the root system that is largely determined by its postembryonic branching capacity. Modulation of initiation and outgrowth of lateral roots provides roots with an exceptional plasticity, allows optimal adjustment to underground heterogeneity, and enables effective soil exploitation and use of resources. Here we discuss recent advances in understanding the molecular mechanisms that shape the plant root system and integrate external cues to adapt to the changing environment.","lang":"eng"}],"author":[{"first_name":"Krisztina","last_name":"Ötvös","full_name":"Ötvös, Krisztina","orcid":"0000-0002-5503-4983","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"citation":{"mla":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45, Elsevier, 2017, pp. 82–89, doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>.","ama":"Ötvös K, Benková E. Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. 2017;45:82-89. doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>","short":"K. Ötvös, E. Benková, Current Opinion in Genetics &#38; Development 45 (2017) 82–89.","ista":"Ötvös K, Benková E. 2017. Spatiotemporal mechanisms of root branching. Current Opinion in Genetics &#38; Development. 45, 82–89.","apa":"Ötvös, K., &#38; Benková, E. (2017). Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>","ieee":"K. Ötvös and E. Benková, “Spatiotemporal mechanisms of root branching,” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45. Elsevier, pp. 82–89, 2017.","chicago":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>."},"publication_status":"published","publication_identifier":{"issn":["0959437X"]},"_id":"1004","pmid":1,"quality_controlled":"1","project":[{"name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I 1774-B16"}],"oa_version":"Submitted Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","volume":45,"publist_id":"6394","oa":1,"date_updated":"2023-09-22T09:48:15Z","scopus_import":"1","publisher":"Elsevier","language":[{"iso":"eng"}],"month":"08","date_published":"2017-08-01T00:00:00Z","date_created":"2018-12-11T11:49:38Z","file":[{"file_id":"6336","creator":"dernst","relation":"main_file","content_type":"application/pdf","success":1,"access_level":"open_access","date_updated":"2019-04-17T08:00:36Z","date_created":"2019-04-17T08:00:36Z","file_size":364133,"file_name":"Otvos_Benkova_CurOpDevBiol_2017.pdf"}],"has_accepted_license":"1","department":[{"_id":"EvBe"}],"intvolume":"        45","status":"public","day":"01","type":"journal_article","publication":"Current Opinion in Genetics & Development","file_date_updated":"2019-04-17T08:00:36Z","page":"82 - 89"},{"citation":{"apa":"Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten, A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>","ieee":"M. Šimášková <i>et al.</i>, “Cytokinin response factors regulate PIN-FORMED auxin transporters,” <i>Nature Communications</i>, vol. 6. Nature Publishing Group, 2015.","chicago":"Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden, Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>.","ama":"Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. 2015;6. doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>","mla":"Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>, vol. 6, 8717, Nature Publishing Group, 2015, doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>.","ista":"Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT, Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E. 2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 6, 8717.","short":"M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten, I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý, K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T. Beeckman, E. Benková, Nature Communications 6 (2015)."},"publication_status":"published","author":[{"first_name":"Mária","full_name":"Šimášková, Mária","last_name":"Šimášková"},{"first_name":"José","last_name":"O'Brien","full_name":"O'Brien, José"},{"id":"391B5BBC-F248-11E8-B48F-1D18A9856A87","full_name":"Khan-Djamei, Mamoona","last_name":"Khan-Djamei","first_name":"Mamoona"},{"first_name":"Giel","last_name":"Van Noorden","full_name":"Van Noorden, Giel"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös","first_name":"Krisztina"},{"first_name":"Anne","last_name":"Vieten","full_name":"Vieten, Anne"},{"first_name":"Inge","full_name":"De Clercq, Inge","last_name":"De Clercq"},{"first_name":"Johanna","full_name":"Van Haperen, Johanna","last_name":"Van Haperen"},{"last_name":"Cuesta","full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Klára","last_name":"Hoyerová","full_name":"Hoyerová, Klára"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"orcid":"0000-0001-5227-5741","last_name":"Marhavy","full_name":"Marhavy, Peter","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","full_name":"Wabnik, Krzysztof T","last_name":"Wabnik"},{"last_name":"Van Breusegem","full_name":"Van Breusegem, Frank","first_name":"Frank"},{"last_name":"Nowack","full_name":"Nowack, Moritz","first_name":"Moritz"},{"first_name":"Angus","last_name":"Murphy","full_name":"Murphy, Angus"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiřĺ","last_name":"Friml","full_name":"Friml, Jiřĺ","orcid":"0000-0002-8302-7596"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva"}],"abstract":[{"text":"Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.","lang":"eng"}],"oa":1,"publist_id":"5513","date_updated":"2021-01-12T06:52:11Z","volume":6,"project":[{"grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","grant_number":"I 1774-B16"}],"quality_controlled":"1","oa_version":"Submitted Version","acknowledgement":"This work was supported by the European Research Council Starting Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.), by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF).","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1640","ec_funded":1,"year":"2015","doi":"10.1038/ncomms9717","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","pubrep_id":"1020","article_number":"8717","ddc":["580"],"type":"journal_article","day":"01","status":"public","intvolume":"         6","file_date_updated":"2020-07-14T12:45:08Z","publication":"Nature Communications","date_published":"2015-01-01T00:00:00Z","month":"01","language":[{"iso":"eng"}],"scopus_import":1,"publisher":"Nature Publishing Group","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"has_accepted_license":"1","file":[{"creator":"system","file_id":"5358","content_type":"application/pdf","relation":"main_file","date_created":"2018-12-12T10:18:36Z","checksum":"c2c84bca37401435fedf76bad0ba0579","file_name":"IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf","file_size":1471217,"date_updated":"2020-07-14T12:45:08Z","access_level":"open_access"}],"date_created":"2018-12-11T11:53:12Z"}]